LOCUS       DACEJR010000001        99882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEJR010000001 DACEJR010000000
VERSION     DACEJR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.001"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1253)
                     /locus_tag="IW651_000001"
     rRNA            complement(<1..1253)
                     /locus_tag="IW651_000001"
                     /product="16S ribosomal RNA"
     gene            1173..1316
                     /locus_tag="IW651_000002"
     CDS             1173..1316
                     /locus_tag="IW651_000002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812769.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            1994..2683
                     /locus_tag="IW651_000003"
     CDS             1994..2683
                     /locus_tag="IW651_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0812770.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            2670..3878
                     /locus_tag="IW651_000004"
     CDS             2670..3878
                     /locus_tag="IW651_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0812771.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            4172..5443
                     /gene="serS"
                     /locus_tag="IW651_000005"
     CDS             4172..5443
                     /gene="serS"
                     /locus_tag="IW651_000005"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0812772.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(5992..7476)
                     /gene="guaB"
                     /locus_tag="IW651_000006"
     CDS             complement(5992..7476)
                     /gene="guaB"
                     /locus_tag="IW651_000006"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0812773.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(7981..8676)
                     /locus_tag="IW651_000007"
     CDS             complement(7981..8676)
                     /locus_tag="IW651_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0812774.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(8766..9866)
                     /gene="ychF"
                     /locus_tag="IW651_000008"
     CDS             complement(8766..9866)
                     /gene="ychF"
                     /locus_tag="IW651_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0812775.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(9875..10066)
                     /locus_tag="IW651_000009"
     CDS             complement(9875..10066)
                     /locus_tag="IW651_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0812776.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(10087..10977)
                     /locus_tag="IW651_000010"
     CDS             complement(10087..10977)
                     /locus_tag="IW651_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0812777.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(10964..11731)
                     /locus_tag="IW651_000011"
     CDS             complement(10964..11731)
                     /locus_tag="IW651_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0812778.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(11743..12456)
                     /gene="rsmG"
                     /locus_tag="IW651_000012"
     CDS             complement(11743..12456)
                     /gene="rsmG"
                     /locus_tag="IW651_000012"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0812779.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(12688..14589)
                     /gene="mnmG"
                     /locus_tag="IW651_000013"
     CDS             complement(12688..14589)
                     /gene="mnmG"
                     /locus_tag="IW651_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0812780.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(14606..16003)
                     /gene="mnmE"
                     /locus_tag="IW651_000014"
     CDS             complement(14606..16003)
                     /gene="mnmE"
                     /locus_tag="IW651_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0812781.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            16347..16685
                     /locus_tag="IW651_000015"
     CDS             16347..16685
                     /locus_tag="IW651_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812782.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(16853..17611)
                     /locus_tag="IW651_000016"
     CDS             complement(16853..17611)
                     /locus_tag="IW651_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0812783.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(17629..18447)
                     /locus_tag="IW651_000017"
     CDS             complement(17629..18447)
                     /locus_tag="IW651_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0812784.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(18444..18800)
                     /gene="rnpA"
                     /locus_tag="IW651_000018"
     CDS             complement(18444..18800)
                     /gene="rnpA"
                     /locus_tag="IW651_000018"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0812785.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(18918..19052)
                     /gene="rpmH"
                     /locus_tag="IW651_000019"
     CDS             complement(18918..19052)
                     /gene="rpmH"
                     /locus_tag="IW651_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0812786.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            19575..20909
                     /gene="dnaA"
                     /locus_tag="IW651_000020"
     CDS             19575..20909
                     /gene="dnaA"
                     /locus_tag="IW651_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0812787.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            21107..22237
                     /gene="dnaN"
                     /locus_tag="IW651_000021"
     CDS             21107..22237
                     /gene="dnaN"
                     /locus_tag="IW651_000021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0812788.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            22466..22708
                     /gene="yaaA"
                     /locus_tag="IW651_000022"
     CDS             22466..22708
                     /gene="yaaA"
                     /locus_tag="IW651_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0812789.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            22695..23819
                     /gene="recF"
                     /locus_tag="IW651_000023"
     CDS             22695..23819
                     /gene="recF"
                     /locus_tag="IW651_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0812790.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            23816..25762
                     /gene="gyrB"
                     /locus_tag="IW651_000024"
     CDS             23816..25762
                     /gene="gyrB"
                     /locus_tag="IW651_000024"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0812791.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            25782..28253
                     /gene="gyrA"
                     /locus_tag="IW651_000025"
     CDS             25782..28253
                     /gene="gyrA"
                     /locus_tag="IW651_000025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0812792.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            28489..28788
                     /locus_tag="IW651_000026"
     CDS             28489..28788
                     /locus_tag="IW651_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0812793.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            28838..29350
                     /gene="ssb"
                     /locus_tag="IW651_000027"
     CDS             28838..29350
                     /gene="ssb"
                     /locus_tag="IW651_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0812794.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            29376..29612
                     /gene="rpsR"
                     /locus_tag="IW651_000028"
     CDS             29376..29612
                     /gene="rpsR"
                     /locus_tag="IW651_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0812795.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            29755..31728
                     /locus_tag="IW651_000029"
     CDS             29755..31728
                     /locus_tag="IW651_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0812796.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            31734..32186
                     /gene="rplI"
                     /locus_tag="IW651_000030"
     CDS             31734..32186
                     /gene="rplI"
                     /locus_tag="IW651_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0812797.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            32460..33827
                     /gene="dnaB"
                     /locus_tag="IW651_000031"
     CDS             32460..33827
                     /gene="dnaB"
                     /locus_tag="IW651_000031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0812798.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            34607..35899
                     /locus_tag="IW651_000032"
     CDS             34607..35899
                     /locus_tag="IW651_000032"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0812799.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            36686..37549
                     /locus_tag="IW651_000033"
     CDS             36686..37549
                     /locus_tag="IW651_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0812800.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(37678..39300)
                     /locus_tag="IW651_000034"
     CDS             complement(37678..39300)
                     /locus_tag="IW651_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0812801.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            39549..40070
                     /locus_tag="IW651_000035"
     CDS             39549..40070
                     /locus_tag="IW651_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812802.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(40256..41986)
                     /gene="spxB"
                     /locus_tag="IW651_000036"
     CDS             complement(40256..41986)
                     /gene="spxB"
                     /locus_tag="IW651_000036"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0812803.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            42151..42969
                     /locus_tag="IW651_000037"
     CDS             42151..42969
                     /locus_tag="IW651_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0812804.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(43072..43269)
                     /locus_tag="IW651_000038"
     CDS             complement(43072..43269)
                     /locus_tag="IW651_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812805.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            43438..44016
                     /locus_tag="IW651_000039"
     CDS             43438..44016
                     /locus_tag="IW651_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0812806.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(45061..45564)
                     /locus_tag="IW651_000040"
     CDS             complement(45061..45564)
                     /locus_tag="IW651_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0812807.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            45922..46947
                     /locus_tag="IW651_000041"
     CDS             45922..46947
                     /locus_tag="IW651_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0812808.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            47270..48148
                     /locus_tag="IW651_000042"
     CDS             47270..48148
                     /locus_tag="IW651_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812809.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            48624..48740
                     /locus_tag="IW651_000043"
     CDS             48624..48740
                     /locus_tag="IW651_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0812810.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            49668..50006
                     /locus_tag="IW651_000044"
     CDS             49668..50006
                     /locus_tag="IW651_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812811.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            50100..50330
                     /locus_tag="IW651_000045"
     CDS             50100..50330
                     /locus_tag="IW651_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812812.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            50730..51101
                     /locus_tag="IW651_000046"
     CDS             50730..51101
                     /locus_tag="IW651_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0812813.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(51375..52067)
                     /locus_tag="IW651_000047"
     CDS             complement(51375..52067)
                     /locus_tag="IW651_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0812814.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(52070..52855)
                     /locus_tag="IW651_000048"
     CDS             complement(52070..52855)
                     /locus_tag="IW651_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0812815.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            53092..54492
                     /gene="lacG"
                     /locus_tag="IW651_000049"
     CDS             53092..54492
                     /gene="lacG"
                     /locus_tag="IW651_000049"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0812816.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            54517..54834
                     /locus_tag="IW651_000050"
     CDS             54517..54834
                     /locus_tag="IW651_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0812817.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            54847..56520
                     /locus_tag="IW651_000051"
     CDS             54847..56520
                     /locus_tag="IW651_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0812818.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            56534..58165
                     /locus_tag="IW651_000052"
     CDS             56534..58165
                     /locus_tag="IW651_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0812819.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(58656..60221)
                     /gene="guaA"
                     /locus_tag="IW651_000053"
     CDS             complement(58656..60221)
                     /gene="guaA"
                     /locus_tag="IW651_000053"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0812820.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(60280..61203)
                     /locus_tag="IW651_000054"
     CDS             complement(60280..61203)
                     /locus_tag="IW651_000054"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0812821.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            61399..62004
                     /locus_tag="IW651_000055"
     CDS             61399..62004
                     /locus_tag="IW651_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0812822.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            62312..63328
                     /locus_tag="IW651_000056"
     CDS             62312..63328
                     /locus_tag="IW651_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0812823.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            63469..64770
                     /locus_tag="IW651_000057"
     CDS             63469..64770
                     /locus_tag="IW651_000057"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0812824.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            64816..65478
                     /gene="deoC"
                     /locus_tag="IW651_000058"
     CDS             64816..65478
                     /gene="deoC"
                     /locus_tag="IW651_000058"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0812825.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            65499..65897
                     /locus_tag="IW651_000059"
     CDS             65499..65897
                     /locus_tag="IW651_000059"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0812826.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            65963..67045
                     /locus_tag="IW651_000060"
     CDS             65963..67045
                     /locus_tag="IW651_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0812827.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            67257..68819
                     /locus_tag="IW651_000061"
     CDS             67257..68819
                     /locus_tag="IW651_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0812828.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            68812..69945
                     /locus_tag="IW651_000062"
     CDS             68812..69945
                     /locus_tag="IW651_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0812829.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            69945..70901
                     /locus_tag="IW651_000063"
     CDS             69945..70901
                     /locus_tag="IW651_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0812830.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            71000..72166
                     /gene="deoB"
                     /locus_tag="IW651_000064"
     CDS             71000..72166
                     /gene="deoB"
                     /locus_tag="IW651_000064"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0812831.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            72190..73008
                     /locus_tag="IW651_000065"
     CDS             72190..73008
                     /locus_tag="IW651_000065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0812832.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            73025..73732
                     /gene="deoD"
                     /locus_tag="IW651_000066"
     CDS             73025..73732
                     /gene="deoD"
                     /locus_tag="IW651_000066"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0812833.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(74030..74716)
                     /gene="gpmA"
                     /locus_tag="IW651_000067"
     CDS             complement(74030..74716)
                     /gene="gpmA"
                     /locus_tag="IW651_000067"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0812834.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(74888..75469)
                     /locus_tag="IW651_000068"
     CDS             complement(74888..75469)
                     /locus_tag="IW651_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0812835.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(75602..76285)
                     /gene="rpiA"
                     /locus_tag="IW651_000069"
     CDS             complement(75602..76285)
                     /gene="rpiA"
                     /locus_tag="IW651_000069"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0812836.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            76604..77017
                     /gene="rpsL"
                     /locus_tag="IW651_000070"
     CDS             76604..77017
                     /gene="rpsL"
                     /locus_tag="IW651_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0812837.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            77101..77571
                     /gene="rpsG"
                     /locus_tag="IW651_000071"
     CDS             77101..77571
                     /gene="rpsG"
                     /locus_tag="IW651_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0812838.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            77665..79752
                     /gene="fusA"
                     /locus_tag="IW651_000072"
     CDS             77665..79752
                     /gene="fusA"
                     /locus_tag="IW651_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0812839.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            79924..81111
                     /gene="tuf"
                     /locus_tag="IW651_000073"
     CDS             79924..81111
                     /gene="tuf"
                     /locus_tag="IW651_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0812840.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            81518..81826
                     /gene="rpsJ"
                     /locus_tag="IW651_000074"
     CDS             81518..81826
                     /gene="rpsJ"
                     /locus_tag="IW651_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0812841.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            81857..82486
                     /gene="rplC"
                     /locus_tag="IW651_000075"
     CDS             81857..82486
                     /gene="rplC"
                     /locus_tag="IW651_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0812842.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            82514..83137
                     /gene="rplD"
                     /locus_tag="IW651_000076"
     CDS             82514..83137
                     /gene="rplD"
                     /locus_tag="IW651_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0812843.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            83137..83427
                     /gene="rplW"
                     /locus_tag="IW651_000077"
     CDS             83137..83427
                     /gene="rplW"
                     /locus_tag="IW651_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0812844.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            83466..84299
                     /gene="rplB"
                     /locus_tag="IW651_000078"
     CDS             83466..84299
                     /gene="rplB"
                     /locus_tag="IW651_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0812845.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            84342..84620
                     /gene="rpsS"
                     /locus_tag="IW651_000079"
     CDS             84342..84620
                     /gene="rpsS"
                     /locus_tag="IW651_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0812846.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            84643..84990
                     /gene="rplV"
                     /locus_tag="IW651_000080"
     CDS             84643..84990
                     /gene="rplV"
                     /locus_tag="IW651_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0812847.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            85004..85660
                     /gene="rpsC"
                     /locus_tag="IW651_000081"
     CDS             85004..85660
                     /gene="rpsC"
                     /locus_tag="IW651_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0812848.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            85663..86097
                     /gene="rplP"
                     /locus_tag="IW651_000082"
     CDS             85663..86097
                     /gene="rplP"
                     /locus_tag="IW651_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0812849.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            86087..86275
                     /gene="rpmC"
                     /locus_tag="IW651_000083"
     CDS             86087..86275
                     /gene="rpmC"
                     /locus_tag="IW651_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0812850.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            86300..86566
                     /gene="rpsQ"
                     /locus_tag="IW651_000084"
     CDS             86300..86566
                     /gene="rpsQ"
                     /locus_tag="IW651_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0812851.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            86624..86992
                     /gene="rplN"
                     /locus_tag="IW651_000085"
     CDS             86624..86992
                     /gene="rplN"
                     /locus_tag="IW651_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0812852.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            87029..87337
                     /gene="rplX"
                     /locus_tag="IW651_000086"
     CDS             87029..87337
                     /gene="rplX"
                     /locus_tag="IW651_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0812853.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            87365..87904
                     /gene="rplE"
                     /locus_tag="IW651_000087"
     CDS             87365..87904
                     /gene="rplE"
                     /locus_tag="IW651_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0812854.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            87923..88108
                     /locus_tag="IW651_000088"
     CDS             87923..88108
                     /locus_tag="IW651_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0812855.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            88144..88542
                     /gene="rpsH"
                     /locus_tag="IW651_000089"
     CDS             88144..88542
                     /gene="rpsH"
                     /locus_tag="IW651_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0812856.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            88574..89110
                     /gene="rplF"
                     /locus_tag="IW651_000090"
     CDS             88574..89110
                     /gene="rplF"
                     /locus_tag="IW651_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0812857.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            89271..89627
                     /gene="rplR"
                     /locus_tag="IW651_000091"
     CDS             89271..89627
                     /gene="rplR"
                     /locus_tag="IW651_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0812858.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            89648..90148
                     /gene="rpsE"
                     /locus_tag="IW651_000092"
     CDS             89648..90148
                     /gene="rpsE"
                     /locus_tag="IW651_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0812859.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            90163..90342
                     /gene="rpmD"
                     /locus_tag="IW651_000093"
     CDS             90163..90342
                     /gene="rpmD"
                     /locus_tag="IW651_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0812860.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            90386..90826
                     /gene="rplO"
                     /locus_tag="IW651_000094"
     CDS             90386..90826
                     /gene="rplO"
                     /locus_tag="IW651_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0812861.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            90826..92121
                     /gene="secY"
                     /locus_tag="IW651_000095"
     CDS             90826..92121
                     /gene="secY"
                     /locus_tag="IW651_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0812862.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            92183..92830
                     /locus_tag="IW651_000096"
     CDS             92183..92830
                     /locus_tag="IW651_000096"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0812863.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            93021..93239
                     /gene="infA"
                     /locus_tag="IW651_000097"
     CDS             93021..93239
                     /gene="infA"
                     /locus_tag="IW651_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0812864.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            93272..93388
                     /gene="rpmJ"
                     /locus_tag="IW651_000098"
     CDS             93272..93388
                     /gene="rpmJ"
                     /locus_tag="IW651_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0812865.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            93406..93771
                     /gene="rpsM"
                     /locus_tag="IW651_000099"
     CDS             93406..93771
                     /gene="rpsM"
                     /locus_tag="IW651_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0812866.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            93799..94188
                     /gene="rpsK"
                     /locus_tag="IW651_000100"
     CDS             93799..94188
                     /gene="rpsK"
                     /locus_tag="IW651_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0812867.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            94268..95206
                     /locus_tag="IW651_000101"
     CDS             94268..95206
                     /locus_tag="IW651_000101"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0812868.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            95234..95614
                     /gene="rplQ"
                     /locus_tag="IW651_000102"
     CDS             95234..95614
                     /gene="rplQ"
                     /locus_tag="IW651_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0812869.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            95886..96725
                     /locus_tag="IW651_000103"
     CDS             95886..96725
                     /locus_tag="IW651_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0812870.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            96701..97570
                     /locus_tag="IW651_000104"
     CDS             96701..97570
                     /locus_tag="IW651_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0812871.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            97560..98357
                     /locus_tag="IW651_000105"
     CDS             97560..98357
                     /locus_tag="IW651_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0812872.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            98560..99306
                     /gene="truA"
                     /locus_tag="IW651_000106"
     CDS             98560..99306
                     /gene="truA"
                     /locus_tag="IW651_000106"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0812873.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        31082 a        18567 c        20741 g        29492 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttttgat tgttatacgg tattagcacc
     1081 tgtttccaag tgttatcccc ttctgatggg caggttaccc acgtgttact cacccgttcg
     1141 ccactcctct ttttccggtg gagcaagctc cggtggaaaa agaagcgttc gacttgcatg
     1201 tattaggcac gccgccagcg ttcgtcctga gccaggatca aactctcata aaaagttcga
     1261 acaatcttgc gattgttaag ctcaatttgt ttgctagcat attgcttgct tgttaaaaat
     1321 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
     1381 gttttcaaag gtctacttca tcaggagtgt ttcccgattc gtttttggtt gccgtagcaa
     1441 cttttatatc atatcagaga gacaagtaat tgtcaactct tttttaaaag ttttttcgat
     1501 ttcgtttttc gaaatcttcc agcaactcat ttatcataac ttatcgtttg ttatttgtca
     1561 agaacttttt tgaagttttt ttcgatagaa tcaagcttcg caagtggttc ttttttaacg
     1621 actcagttat cttatcatgt catttccaac atgtcaacaa cttatttttt attatttaat
     1681 aagttattgg tgttcttttg aacaaataat aatatatcaa catcacatgt gaaagtcaat
     1741 aagaaaatgc ttatttatca acatttctag cttacatgat cattggttag ttctggtcct
     1801 ttttcgttcg tctttataac tccgagagac gtaaaatcct tttgttttca gtttaatgtt
     1861 catgttcttg tcttattcac accaactaga tgtctcacct tctaaaattt tccttaacaa
     1921 agtgctgctt ttatctattt ctgctttttt ttgctagaat aatagtaaca atatgactct
     1981 ggaggattta tttatgaaaa ttttgattgc tgacgatgat aaagaaatcg ttgaattatt
     2041 aagtatctat gttcacaatg aggggtatga agcagtaaag gcttatgatg ggaaagaagc
     2101 attatctaaa atcagaacaa tccctgatat tgaattactt attttagata tcatgatgcc
     2161 tgagatggat gggatgcaag tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat
     2221 tatgctgaca gcaaaaacta ctgatatgga taagatcaaa ggactggtcg ctggtgcaga
     2281 tgattatgtg accaagccat ttaatccgtt agaagtaatg gctagagtaa agtctttatt
     2341 acggcgtaca aaaatgcaga ttgccccaga tcagcctgat gtattagaag taaatgcatt
     2401 aatcatcaat aaagactctc atgaagttaa aacaatcgat gggaaagaaa tccagttaac
     2461 agctttggaa ttcggtatcc tctacttgct tgctagtcat cctaatcgag tatttagcgc
     2521 ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt gtctctgcta aaactgtcat
     2581 ggttcacgtt agtcatttaa gagataagat tgaagaagca actggtggtg aaaaagtcat
     2641 tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga tagtaaacga gcaactgaaa
     2701 aacgccgtcg gatcaatttg acttcaaaag aaatcagcga attgttagcg gaaggaatca
     2761 tcacgattat ccttttgctt ctattaaatg tttctattct ggttgttatc agttcggtga
     2821 tcaacagttc accttctttg acgaatgcga tttgggattc aaagaatatt tttgcagaac
     2881 gattgaacac tgatttgttc tggaacggcc ggaattttat tattcccttt ttctttctat
     2941 tagatattgg tgttttgtat tggcgcttga ttcgaagata tcgccaaatg caattgcggc
     3001 atatcatcag cgaacttcac tacatcgcaa acggaaatta tgatcatcgt atcccttttg
     3061 aactgagtgg tgacttaagc cgagtggtaa caagtatcaa tggattggtg gatagtaccg
     3121 tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg
     3181 tcagtcatga tatccgtacc ccactcacgt ccatcattgg atacctagga ctgatagaag
     3241 atggccagta tcactcagaa gaagatcttt taaagtatac gcatactgct tatatcaaag
     3301 caaagcagat gaaatcttta gtggacgact tatttgaata cactaaggta cgccagcctg
     3361 ctgtcccggt gaatttttct gcctttgaca tgatccagtt gatcgaacag ttagcagctg
     3421 attttgagtt ggaagcttct aaaaaaaata tccaaatact tgttcaatca aaagtagact
     3481 cccttatcat ggatggcgat accgaaaaac ttgtacgtgt cttcaataat cttttgacga
     3541 atgcgttgaa atacggaaaa ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt
     3601 cagaagttgt tgcaacagtc aaaaataacg gagcaatgat cccacaacag gcaatcgata
     3661 atctattcga ccgtttttat cgtgtagaag aatctcgttc acaagcaact ggaggaactg
     3721 gacttggttt agcaatcgct caaagcattg tggctttgca cggcggatac atttatgcaa
     3781 aatcagataa gcaatggact tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat
     3841 tacctattga tacacaggaa gttattgacg aaagctgaaa tattcgctat tcttattaca
     3901 attattagaa taaatgaaaa acacagaacc cagtacactt tattcttttt cagagagctg
     3961 atggcaggtg taaatcagca aaggataatt tggaagttcc agttcgttta cgtgcccttt
     4021 cgaaataaga aaggtcggtt caacagccgt tatgtgttca acaagtgcag ctgacgctga
     4081 acttgggtgg taccgcgaat ttcagactga ttcgtcccaa gaataatggg atggatcagt
     4141 ctttttttat tttcaaaaag gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa
     4201 tattgaagaa gtacaagaaa aattgatgac tcggggcgta aaaaaagaaa cattagctga
     4261 attcatggaa ctagatgaaa atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa
     4321 atatcgaaat gatgtttctg ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc
     4381 tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc aaagcattgg atactgaaat
     4441 tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact ttgccaaacc ttccccatga
     4501 ttctgtgcca gttggaacag atgaagagga caatgtagaa atccgtcgtt ggagcgaacc
     4561 acgtacattt gcttttgaac caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt
     4621 agattttgaa cgtggcgcaa aagtagccgg tagccgtttt gtctattaca aaggattagg
     4681 tgctcgatta gaacgtgctg tctacaactt catgcttgat cagcatattt acgaacacgg
     4741 ctatactgaa atgatcactc cttatatcgt caacagtaaa gcgatgtttg gtacgggaca
     4801 atttccaaaa ttcaaagaag atgttttcca attggagaat actgatctaa cattaatccc
     4861 aacagctgaa gtaccattaa ctaactatta taatgatgaa attttagatg gtgcagattt
     4921 acctatttat ttcactgcac taagccctgc ttttcgttct gaagccggaa gtgctggtcg
     4981 ggatacacga ggactgattc gtcttcacca attcaataaa gtagaaatgg tcaaattttc
     5041 ggatgtagag cattcttacg aagaattgga aaaaatgaca gctgatgcag aagatatctt
     5101 gaaaaaattg catttgcctt atcgtgtgat ggccttatca actggagaca tgggcttttc
     5161 tgcagctaaa acttatgact tagaagtatg gatacctgca caagatactt atcgtgaaat
     5221 cagttcttgt tctaactgtg aagatttcca agcacgccgc gcaatgatcc gttatcgcga
     5281 taatgatgga aaaatccact atactcatac tctgaatggt tcaggacttg cagttggccg
     5341 tacagtagct gccattttgg aaaactacca aaatgaagat ggttctgtca cagttccaga
     5401 agcgctcgtt ccttatatgg ggaatctgaa agttattaaa taaagaggct gtgaaaaagc
     5461 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
     5521 attggactta ataccgagaa actggctttt gaacaccatt tatcgagagg ttgtgaaatc
     5581 agcgaattaa cctgagtatt agagaaaaaa atggaaaaat agtccctcat attttaccgt
     5641 ttgttgaact aataccgagg gtcagctgat tgaacaccgt ttagtggaga ggtcgtaagc
     5701 ctacccccca gctcctagtt acaaggacaa attcaaaaaa cagctcttca tattttatga
     5761 atgttgtact tgtgattcgg aggtggcaag ggaacactgt ttattcaaaa agaggctggg
     5821 acagaagcgt ttaactccaa gaaataagaa gaaattcacg aaaattgctt ttcaaatttt
     5881 tgtgaatttc ttcttatttc cgaaggagtt gcttctactt ccgccgttta tacgtgttta
     5941 gagcgtgaga caaaagtgtt ctttactttt gtcccacgct ctttaatgtt tttaagattc
     6001 aactgaatag tttggtgctt cttttgtgat ttgtacatca tgagggtgtg attcttttaa
     6061 tccactgccg ctcatttgga taaattgcgc ttcatcacgt aattgctgta gattggctgc
     6121 cccaacataa cccattcctg aacgcaaacc gccgatcatt tggaaaataa tatctgcaac
     6181 acttccttta taagcaaccc gaccttcgat tccttctggt actaatttat ttgcttcatt
     6241 gacactacct tggaagtaac ggtcttttga acctttttcc atcgcgccta atgagcccat
     6301 acctcggtat gttttgaatc gacgtccttg gaagatttcg aattcacctg gtgattcatc
     6361 tgtccctgcc aacatacttc ctagcatcac tgcatgtccg ccggcagcta atgctttgac
     6421 gatgtcacca gaatatttga tccctccgtc tgcaataatc gctttgccat attgacgagc
     6481 aactgatgct gcatcataaa tagctgtgag ctgaggtaca cctacaccgg ctaccacacg
     6541 tgtcgtacaa atcgatcctg gtccgatacc aacttttacg acatctacac ctgcatcata
     6601 taaagctttt gtcgcttcag ccgtcgcaac gtttcctgca atcaatgttg catctgcaaa
     6661 agttgaacgg atttcttgaa tttttcggat cacacctgca ctatgtccat gagctgtatc
     6721 gatgatgatt gcatctgccc ctgcttctaa taaagcattc gcacgctcaa atgtatcgct
     6781 tgttacacca acggctgctg ctacaagcaa tcgaccatgt tcatcttttg ctgcatttgg
     6841 aaattcgatc actttctcga tgtctttgat cgtgatcaat ccgcttaaac gaccttcgtt
     6901 atctacgatt ggtaattttt cgattttatg tttttgcaaa atcttttctg catcttttag
     6961 agaggtacct actggggcag tcactaagtt gtcttttgtc atcacatcag caatcggcat
     7021 gtgataatct gttacaaaac gcatgtcgcg gtttgtaatt atccctacca attttctgtt
     7081 ttcaagtgtt tcaacgattg gcacaccact gatacgatat ttgctcatca attcttctgc
     7141 gtctgctaca agatgctgtg gcgttaagaa gaacggatca atgattacgc cactttcaga
     7201 acgtttgact tttcttactt catccgcttg ctggctgatc gtcatgtttt tatggatcac
     7261 acctagtccg ccttgtcttg ccatagcaat ggccatcttg ctgtcggtaa cagtatccat
     7321 acttgcactg atgattggaa tattcaactt aatattttta gctaattgta cgctcatatc
     7381 cacttcgttt ggtaacacgt gactttctgc tggtatcaat aagacatcat caaaagtaaa
     7441 tccttttttc gtgaactttg tttcccagtt ggacattttt agtaccactc ctctaattta
     7501 gtttatctat taaaataatt gattttttca tttaagtcaa tgatctactt ggaatcaagt
     7561 tagctttctc atagaatacc aatataaagc gtttacatct gtgtgtatta aaagagattg
     7621 taaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt
     7681 tttgaattgg ttgggtttat taccgcagat gcaaactttt gaacaccgtt tagtggagag
     7741 gctgtgaaaa agctgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
     7801 catttttgca taaattggac ttaataccga gaaactggct tttgaacacc gtttatcgta
     7861 aaaagggact gtgacaagac ttttgtcaca gtcccttact attatttaac tatggataaa
     7921 caaaatgagg ttctttacta cccctagata tctagcaaaa tagctgatct ttttgctagt
     7981 ttatcttctt gggacggata aacttccaat aatcccgtat tttttcttca agtagtaacg
     8041 gattgctaaa gcagttgccc cgataatgat aactactact ggatctaata ctggattgat
     8101 aactggtgtc aatagtgttg tcgcagaaaa cagtacgacc caaagaagtg tcgttccacc
     8161 taggataagc aatgtcttac ccattcctgg tttcttgctt cgatctgctc cgggatattc
     8221 atattgatag atgtatttgt acatcatata aaagacccag ccaccgattg cagaacctag
     8281 aacgagtgta gaaaagccat aagctggcgt tgttccacgt gaaaacaatc cggtaatcgc
     8341 aatcatgata cttaatagac caagcaacag caacgtatta tctaaccaca tcaataaagg
     8401 ggtggatgaa ttttttactt ctgctggctt attgatgatt gcttccgttc tttcagagac
     8461 tgtcccaaac aattgacggg ctgttcttcc gcctttttgt tctgcaacta aagtaggcag
     8521 aatgtcatga agggctttta ctttttcttc ttcagacaaa ttcgctgctt ctaaagattt
     8581 tttcaaatca aaaatgtact gttgatttct tttggttaat tttgtttcta aactattgtt
     8641 ttcttttacg attgctcgta attgttctgg ttccattatc taatagtcct ccttagccgt
     8701 tccttttctc aacggtcgtc acaaaagaca ttctaccata ttctacagct agtttgatag
     8761 cccttttaca cattaaagcg gaataacatt acgtcgccgt cttgtacgac ataatctttt
     8821 ccttctaatc gaacacgtcc agcttctttt gctgcatgca tgtttccgta atgatccaag
     8881 tcttcaaaag agacagtttc tgcacggata aatccgcgtt cgaaatctga atggatgatt
     8941 cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc aagcacgtac ttcttgttcc
     9001 ccagcagtaa agtaagtagc taagcctaac aaatcataag cagcacggat caactgatcc
     9061 aaacctgatt cttcgattcc taaagcttct aaaaattccg ctttgtcttc ttcatctaat
     9121 tctgcgattt cttcttctgc acgagcactg accacgatca cttctgcatt ttcttctgca
     9181 gcaaagttgc gcacttcttg tacataatga ttcgcgtctg catctgctac gtcatcttct
     9241 gagacattgg ctacataaag aattggtttt gtcgtcaata aaaatagtga tttaacgatt
     9301 ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt ttccttcttc cagtacaggt
     9361 ttgattttat ccagtacagc tagttctgcc actgcatctt tatctttggt tttagcaact
     9421 tttgctacac gagtatagcg tttgttgata gattctaagt ctgctaacac tagttccaag
     9481 ttgatcgtat caatatcttc tagtggatca acacgtcctt ctacgtgagt aatattatca
     9541 tcatcaaaac agcgaacaac gtgacaaata gcgtctactt ggcggatatg gcttaagaat
     9601 tgatttccca aaccttctcc tttacttgcg ccttttacga ttccagcaat atctgtaaat
     9661 tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt ctgttaagcg ttgcaaacgc
     9721 cagtcaggta cttctaccat ccctacgttg gggtcgatcg tcgcaaaggg atagtttgct
     9781 gcttctgctc ctgcttttgt gatcgcatta aacaaagttg attttcctac gttgggcaac
     9841 ccaacaattc cagcagtcaa tgccataaat ttattcactc tttctctgca tttttttcaa
     9901 ttactttttt taatttcttt tcaaaatctc gacgggtcat catgacgata tgtccgcaat
     9961 tcatacatct gattttgata tctgcgccca ttcgaatgac ttcccagcga ttcgtctggc
    10021 aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc gtacataaaa atagcctcct
    10081 ttttgtttat tcttcatcaa attgaatatt caagatatca agaattcgag tcaaatcttt
    10141 ttgagaaaga tattcaatct cgatcttacc tttcccattt ttttcttgga tagctacact
    10201 tgtaccaaat ttatccatca aacgatcttc gctttctcgg atataatatg gtttttcctt
    10261 tgctagcctt gggatttttt tcttttcttt tccatcattt tgatttaact cagaaacaag
    10321 ctgttccagt tggcggacag ttaaattttc agttactgca cgattggcta atttcaagat
    10381 caatgtttta ttttttaaac ctaacagcgt acgggcttgt cccatagata aacgctgatc
    10441 ttgtaccatt tccttgacaa gatccggtaa agaaagcaag cgcaaataat tggcgatata
    10501 aggtctgctc tttcctaaac gactagctac ttctgcttgt gttagtttca ggtttttcat
    10561 cagcatctca tatgcttctg cttcttctaa cggattcaga tcttctcgct gcaagttttc
    10621 cagcactgct acttgcatca tcgcttcttc gtcaaagtct ctaacgatag cagggatcgt
    10681 ttctttttca gctagtttgg acgctcgaaa acgtcgctcc cctgcaataa tctcatatcc
    10741 ttttactgcg gaacggcgta cgatgatggg ttgaaagaca cctgattgtt caatagaatt
    10801 agctagttcc tgcaaagata cttcttcaaa tgttttacgg ggctgatacg gatttggtcg
    10861 cagctcattc aatggtagct ggacaacggt atcatctttt acatccaccg tctctaagtt
    10921 ttctaaatct tgaaacagtg catcgattcc tcggcctaat cctttacttt tcttcacgac
    10981 ttaccacttc ctttgccaat gcttggtaga cttcagctcc tcgagaacgt ggatcgtagt
    11041 cgatgatcgg ttttccatga ctaggtgctt ctgataaacg aatatttctt ggaatgatcg
    11101 tttcgtaaac tttttcgcgg aagtatttac gtacttcttc tacgacttca tttcccagat
    11161 ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat ctctaattct ggattaaagt
    11221 gtttttggac taaacgaacc gtattcagta attggcttaa tccttccaat gcgtaatact
    11281 cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa tgaattgatt gtcagatggc
    11341 caagagaagg cgggcaatcg atcaaaatat aatcatactg actgcttact tctgctaaag
    11401 atccttttaa tcttgattct cttgccatca ttgacgtcaa ttcgatctct gctcctgcta
    11461 attggagagt cgcaggcaca atactcaaat tttcgtgttc agtgatcaaa gttgcctcat
    11521 caattggcaa ttcattgact agaacatcat agatgtctct tgtcacatca ggttttctga
    11581 tcccgacacc actggtagca tttccttgtg catccatatc cactagcaat acttttttgc
    11641 caaggctagc aagacaagca cctagattca ccgtcgtcgt tgttttgccg acgccacctt
    11701 tttggttcgc aacagatatt atttgtgcca tctcttctcc tcctatttga ttggctgctt
    11761 attaggcagt ccgggctttc ttgggtattt ctttggggtt ggtttctttt tttgaataac
    11821 gatgatatgc cgttgatcag agacattcgg caaggtaacc gcctcttctt tgaggaattt
    11881 accacctaat aaagcaatcg cttgttttgc ttctgtcaat tcctcttcac tttttgctgc
    11941 tttcaatgca taaaaatagc cttcttgttt agccaagggc aaacacaatt ccgctaacac
    12001 attcaaacga gcaaccgcac gagcagtgac aaggtcaaat gatgcacgaa agtgaggatt
    12061 ttgcccaaat gtttctgccc ggtcatgata taaggaagca tcgattccta ataatcgggt
    12121 taactcagat aagaaggtta ttcgtttatt cagagaatca acgatcgtta tctgcagttc
    12181 tggaaaggca atttttaaag gtatgctagg aaaccctgcc cccgaaccaa catcgcataa
    12241 agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta agtgaatcgt aaaaatgctt
    12301 cagatatacg tctttttcat ccgtaatagc tgtaaggttc atcttttcgt tccattcgac
    12361 taatgtccga taataaatag cgaattgatt catttgatga tcggtaagct ctatcccttt
    12421 ttctgccaag gattggcgaa attcttctgg tatcataaat attcctttca ttgtgaaaca
    12481 gcaattgttt cacagattct ctccctactt taacgcaaaa tctctagaca tgcaatcctt
    12541 ccatatggat tcttaaaaat ttgaaaagta agaaaagccg atagactttt tctttctagt
    12601 atgactcata aaatatacac agaagagaga actagctttg agcaatttct gcaaaaacta
    12661 gttctctctt ctatttatta ttacgtatta cgctggttca ctgactttcg caatcttccc
    12721 ttgttcgata tagaccatta ggatacttag gtctgcaggg tttacgccac tgattcggct
    12781 agcttgggca attgtttctg gttggatttt ctgcaatttt tgttttgctt ctgttgctaa
    12841 tccgttgatt gctgcgtagt cgatgttttc tggaatcttt ttcgcttcca tgcgttttaa
    12901 tttttctact ttttccatgg cttttttgat gtagccttca tatttgattt ggacttctac
    12961 ttgttcgatt tcttttcgtg tcaattcttc attttctgga ataaaccgta gaagttctgc
    13021 ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt acaccatctt ttaatggtgc
    13081 cgcattaatc gttgctaaaa agtcttggac ttcttctgtt ggtttgattc gtacattcat
    13141 caaacgtttg atttcttgtt caactgcttc ttttttcttc aaataattct tgtattgatc
    13201 ttcttttact aagcctacac ggtgacccat ttctgttaat cgcaaatctg cattatcatg
    13261 acgtaaaagc agacggtatt cagcacgtga agtcagcaaa cgataaggtt cgttcgttcc
    13321 ttttgttacc aaatcatcga tcatgacacc gatgtaaccg tcacttcgtt tgatcactaa
    13381 aggttctttt ccctgaactt tcaaagctgc attgataccg gcaatcagcc cttgtccagc
    13441 cgcttcttca taaccgcttg taccatttgt ttgtccagct gtataaagat tttcaacgac
    13501 tttagtttct aatgtcggac ggagctggtg tggctctact acgtcatatt cgatagcata
    13561 tcctgtacgc atcatttcta ctttttctaa tccagcaata gaatgaagca tgttgacttg
    13621 aacatcctct ggtaaagaag tagataaacc ttgaacatag acttcttccg tatttaagcc
    13681 ttctggttcc aaaaataatt gatgacgtgg tttgtctgca aaacgcacga tcttatcttc
    13741 gatggatgga caatagcgag cgcctacccc ttcaacgatc cctgtaaaca ttggggcacg
    13801 atgtaagttg tctcgaataa tttggtgagt cgtctcacct gtataggtca gccagcaaga
    13861 ttcctgatct agtttatagg cactatctgg tgtgctgaag ctaaaatgat tcggctcttt
    13921 gtcaccaggc tgctcttctg taacagaata gtcgatcgta cttgatttta ctcgaggcgg
    13981 tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct tttagatgat tcgctagtcc
    14041 aatagaaggt tgagagttat tcggacctga tgaatatttc aattcaccga taatgatttc
    14101 tccgcgaagt gctgttccgg ctgtaataac aactgctttg gcccggtagc tcgctcctgt
    14161 ggacgtcact actccatgac aaacaccatc ttcgacgatc agttcttcga cgattccttg
    14221 tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc atttctgttg aataagcatg
    14281 tttgtctgct tgtgctcgca atgctcggac agctgggcct tttcctgtgt tcaacatacg
    14341 catttggata taggttttat cgatattctt acccatttct ccgcctaacg catcgatttc
    14401 tcgaacgaca acgcctttag ctggtccgcc tacagaagga ttacatggca tgaatgctac
    14461 catgtccaaa tttattgtca gtaataatgt ttttgatccc atacgtgcag cagcaagtgc
    14521 agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg tcatacgcat ctgcttcata
    14581 atgttccaat cgtttgttcc tccttttatt ttcctaaaca aaattgactg aataattgag
    14641 tgattagctc atcttgtaca ctgtcgccga caacttctcc tagatagtcc cagcaacgcg
    14701 tcatatctat ttgaacgaga tccacaggca ttcctgaatc aatacctgca atgacttctt
    14761 caagtgagag ggaagctttt tctaacaacg cgatatggcg ggtattagaa acataagtag
    14821 cgtctctttc tcctgtttct ccgctgaaga ataaatcaga aatagctgat tccaaacgat
    14881 ctagtccgtc gtttttagct acggaaattg aaaaaactgg ttcattttcg atcaatttct
    14941 tcaatttttc ttgttccagc tgtgctggca aatctgtttt atttagaaga atgatccgtt
    15001 tcaatccact tgtcgcttcc aataattgtt catcttctgc tgtcagtggt tcgctttggt
    15061 ttagaacaag taagatcaag tctgcttctg ctaatgcttt tcggcttctt tcgaccccga
    15121 tccgttccac cacatcttct gtttcccgaa ttccagctgt atcgattagc ttgagcggta
    15181 ccccccgaac attgacatac tcttcgatta catctcgagt cgttcctgca atatccgtaa
    15241 cgattgcttt ttcttctctt aacaaatggt ttaacaaact tgatttgccg acatttggcc
    15301 ggccgatgat tgccgtactt aacccttccc gcagaacctt cccttgcttg gatgtagcaa
    15361 gtagcgcttg gattctttgt tggatcatct gcgctttttc aagcaataac ttcgtagtca
    15421 attcttcgac atcatcatat tctggataat caatatttac ttctacttgt gctaatgtct
    15481 ctaaaatctc ttgtctcaat gagcggatca aagcagacag attgccgtct aactgattaa
    15541 gagctagtcc cattgctttg tctgtttttg cccgaatcaa atccatcaca gcttctgcct
    15601 gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt gaattcgcct ggctcagcta
    15661 atcttgcacc ttctcttaac aatagctgca aaatttgatt gactacaacg atcccaccgt
    15721 gacaattgat ttctactaca tcttcccgcg taaatgtctt tggtgctctc atcacggaga
    15781 ccatgacttc atcgacaagc tgattgctct tgggatcaac gatatgtcca taatggatcg
    15841 tatgacttgg tacttctgat aatcgtttgt ttccagattg ataaacttta tctgctagtt
    15901 gcacagcttg atcgccactt aaacgaacga tactgattgc accttctcca ggtggggtag
    15961 aaattgccgc aatcgtatca aattctaaag taatttcagc cattttttca cttccttttt
    16021 cattttgcgc acaaaaaaag tgcccactcc accctttatg cacaataaac ggtgaacttc
    16081 gcactttgac tgcttgttct tgattaaatt ctggtcatag attatcacac ttgctataag
    16141 gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt tctgcttcca ttaccttata
    16201 tttaattgtc cttggatgtc tctttgcgtt ctttggttta ttcgaagaga agatactgta
    16261 taaacaaggc tttatctttc ttaaaaaaaa cggtacacta aatggtagcg ctatctaaac
    16321 tatacacttt tgaaaggaga aaatatgtga gcccgttcag atctgctgca ttggaagaag
    16381 aaagtgaccc tcggatcaac gatgtcgttt tatctacagg gaatatcaac cagaaatata
    16441 tcgtccgcga tatcgttttt gttgtggaat gcttagaagc tgatttgttt gacccatctt
    16501 ttcccaaaga tgaccttttc ttaacaacga agaggaaatt aaagacgaag gtacttgaat
    16561 atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca gcaaaaaaaa cgcgacggaa
    16621 agtattttct ggaacttttt gcttatggaa cagtgatcca atttatccaa acaacgattg
    16681 gttaaatgaa acagacaaaa aaagcggatc aaaactgaag acagtttcga tccgcttttt
    16741 tctatcaaag ttagtgacta cttcatcgta tactctgata agaatgattt ctagtaaaat
    16801 atacttggtt tgaaaaactt tgtcaacgtg atttattcaa aaagtaatgg aatcaataat
    16861 attttttagc aggttctact actaaataac gataaggttc ctcaccttcc gaatgtgttt
    16921 ttacatgttc atctttgctt aatgcagcat ggatttgttt acgttcaaat gcaggcattg
    16981 gttctaagaa aacaggctgc cctgtccgtt ttactttttc agccgtgcga tcagctaaac
    17041 gctgaatgat ctcttggcgc ttttcacggt aattgcctac attgactact acagacagct
    17101 tatttcttgc taaacgatgg atgaagactt gtgccagata ttgcaatgca ttcagtgttt
    17161 ttccatgttt cccaatcagt atgccttgct tttgtgtttc taaatggaaa acaaccatat
    17221 tatttgattc tctttccatt ttgacaagag ctggagcatc aagctgtttt gaaatttcag
    17281 taagataaac tgccaattcc gttaaagctg cttcatcacc tagctcactt acttcttctg
    17341 ttttttcttc aagaatcgtt tcgttgacta cctcgatctc agctgttggt tcttcttcca
    17401 aaatttcttc tacagtctgt tctactgttt cagaaacttt ttcgctgata cttggctgga
    17461 tcgaaagccg tgcattcttt cgacccattc ctaggaaacc tttttttcct tcttccagta
    17521 tttcgatttc gacttggtct ttggccaaac ccaaatcagc cagacctagt tggacagctt
    17581 cgtctattgt tgcagcttca taaatcggca ttagcattac ctcccctatt atttccgttt
    17641 tttcttagga ttcattgctt tcttaagagc acgttctctt tcgcggattc tccgtgcttc
    17701 ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc agcagcgttt gtacaacttg
    17761 gaaggcattg gaaactaccc agtataatga aagaccgctg gctaaattga cacccatcac
    17821 taaaatcatt acaggcatca cgaaattcat gatcttcatt gatgcattcg attcaatttg
    17881 actcatgcta gataaatacg tacttgcaaa agtaaagatt gctgctaaaa tcggcaaaat
    17941 aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa tgcccttgtt tcaactctgg
    18001 tacacgtgaa atagattgcc ataacgccat caagattggc atttgtacta ataaaggaag
    18061 acaaccagca tatggattga cgccattttc tgcatataaa cgctgctgct cttctcttaa
    18121 cttactttgt gtttccggat ctcttgaact atattgttgc tgcagtgctt ttaatttcgg
    18181 ttgtaattct tgcgttttac gcatgctctt tgtttggaaa tgcatcaacg gcaataggat
    18241 cacgcggatg atcagtgtaa ataaaatgat ccctatccct gcattaccaa atgacaacgc
    18301 cttgatcgcc tcagcaaagt aataaacaat ataacgatcc caaattcctg tactctgtgc
    18361 gctgacttcc ccagttccgc aaccggataa gacgatgacc aaactaacga taccggccat
    18421 caaaagtaaa cgtttgtatt tcttcactta ctcttttcct ctctgtcgat tatttttgca
    18481 agttttaaga cgtggactaa attggagcga acctcatcat aagtcaaccc cttcacactt
    18541 ggtctagcaa tcacaataaa atccaactcc ggatcaattt cttcttttat agaataaact
    18601 gctgctcgga tttttcgttt gacttcattg cggtttaccg cgttgccgac cttttttcct
    18661 acagataaac caactcgaaa atgaggctgt cccggttttt ccaaacggta aacgacgaat
    18721 ttgcggttag caaaagaatc tcccgtatgg aacacggctt gaaactctcg ttcttttttt
    18781 actcgatacg cttttctcat tagttttctt tccttttcta tctctcactt acgctcataa
    18841 tcataccata attacgattg tcctttcatc atttccctaa aaaagagtat aaaaaaaacc
    18901 actgaaaccc cagtggccta agctgaaaga acttttcttc ctttgcgacg acggctagct
    18961 aacacacgac gtccattttt agtactcata cgtttacgga atccgtgaac tttttgacgt
    19021 ttacgtttat ttggttggta tgttcttttc atatattcca cctccaaaat gttcttatct
    19081 attttataaa cacagacata ctttgatagt ataacgacaa agtagtgatt ttgtcaatat
    19141 aaaatagaag atcgattgtt aaaacttatc cacaagatta tttttttcta tgggaatcct
    19201 atgtggaaaa agttatgcac agggattatc cacagataat aaaaggctgg ttttacacag
    19261 aaaacacacg tgtaaaaaaa gtaatccaca atcaggcgat ttcctgtgta ttccgtctcc
    19321 taactattgg tatatctgta ttttttatcc acagacgcaa gaggaaatct attggttttt
    19381 acgagttttc cacctgtgca ttagtggtgt ggaaaacttt ttttgacaac tgtgtttttc
    19441 tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt ttcttcggtt
    19501 tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact ttttaatgaa
    19561 ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa gcaacatacc
    19621 aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac cctcgaaccc
    19681 ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa tattgggaaa
    19741 aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat gagattatcc
    19801 ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa aaaaatccaa
    19861 aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat acctttgata
    19921 cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt gtagcagaag
    19981 atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt ggtaagactc
    20041 acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc aaagtaaaat
    20101 atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac aaaacagcag
    20161 aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac attcaatttt
    20221 ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc ttgtacaatg
    20281 aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg aaactgcaag
    20341 aacgccttgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg ccggatcttg
    20401 aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag atcccagatg
    20461 acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta gaaggtgcgc
    20521 ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact agtctagctg
    20581 cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca atcctgcaga
    20641 tccaagaaga agtggcaaag tattatcaca tccaattgaa agatctcaaa ggaaagaaac
    20701 gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt gagctcacag
    20761 ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca acggttatcc
    20821 atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa aatgaagtat
    20881 cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga aaatagcatg
    20941 tggataaaag aaaaaaacgt tcaaaagtta tacacacttt ttccacagct gttttttcgt
    21001 ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac ttttattatt
    21061 ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga aagttacttt
    21121 aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt caagcaaaac
    21181 cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt tgactttgac
    21241 ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg aaaaagcaaa
    21301 tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta gcgaaatcat
    21361 tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac aagtagcgat
    21421 cacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact atcctcatct
    21481 tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga ctaaactaat
    21541 caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga caggtgtgca
    21601 tttcatttta tctgataatt ctttattagc tgtagctact gattctcacc gtctaagtca
    21661 acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc ctggaaaaag
    21721 tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa tcagcattat
    21781 ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt tgctagaagg
    21841 aaactatcca gataccaatc gtttgattcc ttcaagtttt aatacagagg ttgaattttc
    21901 tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg aaggccgtaa
    21961 caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa attcgccaga
    22021 aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc cattagatat
    22081 ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata tgagcatcaa
    22141 agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag atggcgttca
    22201 atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcaa tgttcttgaa
    22261 aatttaattg aaaagaaata gaaaaaataa aattttaaac agaaggccct aaaatctctt
    22321 ttaaggcttt ctgtttattt ttgtttaaat cacctttttt agattaaatc gtcttatatg
    22381 gaaaaatata tggataaatt tgttttattg cgaaaaaaaa ggtataatag agtatacttt
    22441 taataatgaa aatgagggat gggcattgaa acaacaagtc attctaaata cggaattcat
    22501 gacgttgggc caaatgttga aagaagtcac agttatcggt agtggcggac aagcaaaatg
    22561 gtatttggca gaaaacactg tgttagtcga tggcgaacca gaaaatcgtc gtggacgcaa
    22621 actttatccc ggcatgatgg ttgaagttcc agaagtagga acttttttta tggtaaaaaa
    22681 aggaaacgac gataatgagg ctgaataagc tgtatttaaa aaattatcga aattacgaag
    22741 agctgaacat cgaattttcc aagaacctcg tcatcttttt aggagaaaat gcgcaaggaa
    22801 aaacgaatct tttggaaagt atctacgtat tagctatgac gcgaagtcat cggacaagta
    22861 gtgagcagga attgattggc tggactgacg atcaggcgat gattcaagga gagatcacaa
    22921 aaggttcatc cactcttcca ttagaaatct tattatctaa aaaaggccga aaaaccaaga
    22981 tcaaccatat cgaacagaaa aagttaagta cttacgtggg acaaatgaat gtcattttat
    23041 ttgcaccaga agatctttct ttagtaaaag gcagcccgca gatcaggcgg aagtttttag
    23101 atatggaatt aggccagatc aatccaatct atctttatga tcttgtccaa taccaagcaa
    23161 ttttgaaaca aagaaaccag tacttgaagc aattgaatga aaaaaaacaa actgatttgc
    23221 tttatttgga tattttgtcc gaacaactag cagcgtttgg cagcaaagta ttgaaagcaa
    23281 gagcacagtt cgtcaagcga ttggagtttt gggctaattc tttgcatcag caaatcaccc
    23341 atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt cgattcttta gaaacacata
    23401 cgcaagagca aatccaagaa caatttctcg cacttttgaa ccaaaataaa aagaaagacc
    23461 tttttcgggg aacaacgaca gttggtcctc atcgtgacga tctttccttt tttattaatc
    23521 agaaaaatgt gcagacttat ggatcgcaag gacagcaaag aacaactgca ctaagtgtca
    23581 aattagcaga aatcgatttg attaaagaag agacagggga atatccaatc cttttgttag
    23641 atgatgtgat gagcgagctg gatgataatc gtcaactcca tctgctagaa acaatcgaag
    23701 gaaaagttca aacgttctta acaacaacga cacttgacca tgtaaaaaat aaaatgactg
    23761 ttgaacctga gattttttat gttcaacaag ggaaactaaa aggagaagaa actgaatgac
    23821 agaagaaaga agtttagtag aacgcgctaa agagtatgat gccagtcaga ttcaagtact
    23881 tgaaggactt gaagccgttc gtaaacgacc tggcatgtat atcggttcga ctagttctga
    23941 gggtctgcat caccttgttt gggaaattgt cgataattcg atcgatgaag tattagcagg
    24001 ttttgcaacg aaaatccatg tgattattga aaaagacaac agtatcaccg ttattgacga
    24061 cggtcggggg atacctgtag atatccaggc aaaaactgga cgtcctgcag ttgaaacggt
    24121 ctttactgtt ttacatgctg gaggaaaatt cggcggtggt gggtacaaag tttctggtgg
    24181 acttcacgga gtaggatctt ctgttgtaaa cgcgctttct acacagcttg atgtcaaagt
    24241 atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt ggagctgtag tggacgactt
    24301 aaagattatc gaacaaacgg atcgccatgg aacaacggtt cactttacgc cagaccctga
    24361 aatttttaca gagacaactg agtttgattt ttcaaaattg gctacccgga tccgtgaact
    24421 tgctttcttg aaccgcggga tgcgtatatc gattgaagac aaacgagaag aagaaccagt
    24481 catcaatgaa taccattatg acggcgggat caaaagttat gtcgaatact tgaatgcaaa
    24541 caaaaccgtg atttttccag aacctgtata tttggaaggt gaacagcaag atatcgcagt
    24601 tgaagtctct atgcaatata cagacggcta ccattccaat atcatgagtt ttgccaacaa
    24661 tatccatacg tatgaaggtg gaacacatga atcaggattc aagactgctt taactcgtgt
    24721 gataaatgat tatgcccgaa aacaaaagct gatgaaagaa aatgacgaca acttgaccgg
    24781 tgaagacgtg cgagaaggat tgacagctgt tatttctatc aagcatcctg accctcaatt
    24841 cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta cggacagtca ctgatcggtt
    24901 atttagcgaa catttcatga aattcttgct ggaaaaccca agtgtgggcc ggcaaattgt
    24961 tgaaaaagga ttactagctt ccagagctcg tctagcagcg aaacgtgcgc gtgaagtaac
    25021 gagacgtaaa ggtgctttag agattagtaa tctgccagga aaattagcgg actgctctag
    25081 caacgatcct gaaaaatgcg aattgttcat cgtcgaagga gattcggccg gcggatcagc
    25141 taagcaaggt cgtaatcgag agtttcaagc catcttgcct attcgtggga aaatcttgaa
    25201 tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa gagatccggt ctttatttac
    25261 tgctatgggt acagggtttg gagaagattt tgatgtatcg aaagctcgct accacaaatt
    25321 agttatcatg actgatgccg atgtcgacgg cgcgcatatc cgtacattgc tgctgactct
    25381 tttttatcgt tacatgcgtc ctattgttga agctggatac gtctatattg ctcagccacc
    25441 tttatacggg gtaaaacaag ggaagaatat tacgtatgta caaccaggaa aaaatgcaga
    25501 agaagagttg aaacaagtcg ttgcttcact tcctgcctca ccaaaaccaa gcgttcagcg
    25561 ttataaagga cttggagaaa tggatgatca tcaactatgg gaaacaacta tggacccaag
    25621 caatcggatg atggcccgcg tcagtgtgga cgatgctatt gcagcagatc agatatttga
    25681 aatgttgatg ggggatcgag tagaaccccg tcgtgcattc atcgaggaaa atgcacacta
    25741 tgtgaagaac ttagatattt aatcatgaag gaggaaaaag aatgagtgaa gaaatcagag
    25801 aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga ctccttcatc gattatgcga
    25861 tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga tggtctgaaa cccgttcatc
    25921 gccgtatttt atacggcatg aacgaattag gtgtaacacc ggataaagcc cataaaaaat
    25981 ctgccagaat cgtcggagat gtcatgggta aataccatcc tcatggagac cgtgcgatct
    26041 atgagtcaat ggtacggatg gcacagccat tcagctaccg ttatatgcta gtggatggtc
    26101 atggaaactt tggttctgtt gacggtgacg gtgccgccgc tatgcgttat accgaagcac
    26161 ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa taagaacact gttgatttcc
    26221 aaagcaacta tgatgataca gaaaaagaac cagttgtact acctgctcgc ttcccgaatc
    26281 ttttagtgaa cggaacaacg gggatcgccg taggtatggc cacgaatatc ccacctcata
    26341 acttgtctga ggtagtagcg gctatcgatt tgctgatgga taatcctgat gtgacgacca
    26401 atgaattgat ggaagtatta cctggaccag attttccaac aggtggtttg gttatgggga
    26461 aatcaggaat ccgaagagcg tatgaaacag gtaagggttc tattaccgta cgtgcaaaag
    26521 ttgagattac tgagatgcca aatggtaaag aacggatcat cgtgacagaa ttgccttata
    26581 tggtcaataa agcaaaattg atcgaacgga tttctgaact gcacagagac aagcgtatcg
    26641 aagggatcac agatttgcgg gacgaatctt cccgtgaagg tatgcggatc gtcattgaca
    26701 tccgtcgtga cgccagcgca tccgttatct tgaataactt atataaattg acatccttgc
    26761 aaaattcatt tggattcaat atgttggcga tcgaaaaagg tgtgccgaaa gttttgagct
    26821 tgaaacaaat ccttgaaaac tatatcgaac atcaacgcga agtcatcaca cgacggacgg
    26881 tctttgaaaa agaaaaagca gaagcaagag cacacatctt agaaggtttg cgtattgctt
    26941 tggatcatat cgatgaaatc attgcgatta tccgcgggtc taaatcagat gacgaggcaa
    27001 aagcaacaat gatcgaacgg ttcgatttat ccgatcgcca gtctcaagct attttagata
    27061 tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat cgaaaatgaa taccaagaac
    27121 tcttaaaact gattgaagat ttaactgaca ttctggcaag accagaacgt gttacagaaa
    27181 tcatcaagaa tgaattagca gacttgaatc aacgatttgg tgataaacga cgcacagaat
    27241 tactagtcgg tgaggtctta agtctggaag atgaagattt aatcgaagaa gaagaaatcg
    27301 ttattacatt gacaaacaat ggttacatca aacgtttggc aaataatgaa ttcagagctc
    27361 aacgaagggg tggacgtggt gtccaaggaa tgggtatcca caatgacgac ttcgtcaaaa
    27421 atctggtttc ttgttctacc catgatacat tgttattctt tacgaataac ggaaaagtgt
    27481 atcgggcaaa aggatacgaa attccagaat acgggcgtac agccaaagga atcccaatca
    27541 tcaatttact aggtatcgat tcgagtgaaa gcatccaggc aatcattgcg gtgacgggag
    27601 aagcagaaga aggccactat ctcttcttta cgacgcgtca aggtacagta aaacgtactt
    27661 ctgtcactgc ttttgctaac atccgaagta atggtctgat cgcaattggt ttgaaagaag
    27721 acgatgagct cgtcaatgtg ctactgacag atggtcaatc taatatcatt attggaacac
    27781 ataacggcta ctctgtcact ttcaaagaag aagcagtccg cgatatggga cgtacagctt
    27841 ccggggtcag agggatacgc ctgcgtgagg aagattatgt cgttggggca gccttaatga
    27901 aggaaaatca agaagtatta gtgatcacag aaaaaggtta cggaaaacgg acaaaagtat
    27961 cggaatatcc agtgaaaggc cgcggaggta aagggatcaa aacggcgaac atcactgaaa
    28021 aaaatggtcc actagctgga ttgaccactg tttctggaga tgaagacatc atgctgatta
    28081 cagataaagg tgtcattatc cgtttcaacg tatcgactgt ttctcaaact ggacgttcaa
    28141 cattaggcgt acgtttgatg aagatggaag ctgatacgaa agttgtcacg atggcagcag
    28201 tcgaaccaga agctgcagaa caaacagttg aagaacaagg atcagaagaa taaccatcaa
    28261 gactaagtgt gtaagctagc aagaataacc gattaaaaaa tctggttatt cctgctggct
    28321 tttttgatta gttgaatttc tcttaaaata ttcgaaactg ttcattctgt attgcttttg
    28381 accaaaatag agtgtataat tagagcttgt gagtagtcta attaattaga taactctcct
    28441 tgctcttagg aacctaagag ccgaaagtcc acagggaggt gaatatcaat ggaaaacacg
    28501 aaatatgaaa tcatgtacat tattcgtcca aacattgatg aagaagcaaa aactgcttta
    28561 atcgaacgtt tcgatgcaat cttgaaagat aacggagcag aagttatcga atcaaaagat
    28621 tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc gtgaaggtat ctatcacatc
    28681 gttaaagtat cttctccatc atcagcaaat gctgtcaatg aatttgaccg tcttgctaaa
    28741 atcaacgacg atatcattcg tcacatgatt gttaaagaag aagcttaatt gttgtttcat
    28801 gtgaaacaat agaaaacgat gagaggagat gactattttg attaataatg ttgtattagt
    28861 tggacgtctg acgaaagatc cagatttgcg ttacacagca agtggtacgg cagtcgcaac
    28921 atttactttg gctgtgaatc gtaattttac aaaccaaagc ggcaatcgcg aggctgattt
    28981 tatcaactgt gtgatttggc gcaaatcagc agaaacttta gcaaactatg ctcgtaaagg
    29041 aacactttta ggtgttactg gacgtatcca aactcgttct tatgataatc agcaaggaca
    29101 acgtgtatac gtgacagaag tagtagctga caacttccaa ttgctggaaa gtcgttctgc
    29161 atctgaaaat cgccaacaaa gcggcggatt ccaaagttct ggtcaatcag ctggcggatt
    29221 tggcggaaac aataactcta atcaaacatc tcaatcatcc aacggtatgc ctgatttcga
    29281 ccgtgatact tctgacccat tcggctcaag ttcaacgatc gacatttcag acgatgattt
    29341 accattctaa acgaatttga taggagggaa atagaatggc acaacaaaga agaggcggac
    29401 gtaaacgtcg taaagtagac tacattgctg ctaaccatat tgaatatatc gattataaag
    29461 atatcgaatt actaaaaaga tttatttcag aacgcggtaa aatcttgcca cgtcgtgtaa
    29521 ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc aatcaaacgc gctcgtatca
    29581 tgggcttgct acctttcgtt agcgaagact aataaatcct aggaattgaa tgactgatcc
    29641 ttttgatcag ctattcaatt cctttttttc tgtaaaaata acctctattc gtttagtcgc
    29701 tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat atgggaggaa aattatggaa
    29761 aataaaaaca atcatttatc cccgaaacct ctcttcctta tcctgtttat attaatcgaa
    29821 gtattggtag tcttaatttt accatttcgt tttgttagcg cttcggtcat tctattgatg
    29881 aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt ggcttatttt aaataaacgt
    29941 gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc tagcttatgt ctcagaagtc
    30001 atgcctgtag gagtgattgc ttatcagcca tcaagccaaa aaatcgaatg gctcaatcca
    30061 ttagcattga aaatcaaaga tcaaagtgga ttaacagagg aagaattgct agatactttt
    30121 tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag cagataaaac atttcagttt
    30181 gatatggata cagtaaaggg tgttgtttat tttaatgata ttacccaaaa ccagttgtta
    30241 aaactccatc agacgaacag tcagccggtg attggtattt tatctattga taactatgca
    30301 gatgccattg ataaattaga tgacaaagag atttcttatt tgaactcttt cgtcactaca
    30361 ctagtttctg actggatgaa tgaataccat gtctttttca aaagaatcaa cgcagaacgg
    30421 tttttcttcg tagcacagat gatcgatctg aaaaagatga tggataaaaa atttgatttg
    30481 ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc ctatcaccct tagtatgggg
    30541 attgcttatg gctcaagaac cctcgatgaa atcggtggag aagctcagaa taatttagat
    30601 gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag aggtaaaaga aggagcaaaa
    30661 cctgtttatt atggggggaa atctgctaag aatgtcaaac gcacgcgtgt tcgttctcga
    30721 gcgatgagta ccgctttgcg tcggattttt gatgaaacag gcgatgtgtt tatcatgggg
    30781 cacaagtttc cggatatgga tgctttaggc tctgcttttg gtgtcagttg tctggcacgc
    30841 ttcaaccaga aaaaagctta catcgttatc aatgagaacg agatcattcc agatgtagaa
    30901 cggtgtcttg aagaaatcca taagcatcct gaattagaaa tgcaactaat ctctccagaa
    30961 cgtgccatag agctgaaaaa agaagaggac ctcctcgtga tggtggatta ccataaacct
    31021 tctctatcga tctctcaggg cctatatgag caatttgata agattgtgat cattgatcac
    31081 catcgtcgtg gggaagagtt tccaagtaaa cctctgctga cttatattga atcttcagct
    31141 tcttcagctt cagagctagt ttctgagctg atccaatacc aaagcagcaa aacaaatcgc
    31201 ttagatcgtt ttgaggccac attattgctt gcagggatgg ttgtagatac aaatagtttc
    31261 aatgtccgta caactgcacg aacttttgac gttgccagct atttaaggtc ttgtggagca
    31321 gacgcatcct tagtacaata tttattgagc tcggatttag cgacatactt ggagatcagc
    31381 aatttgattg ctggaagcga ctatgtgact tctgatattg ttgtggcggc agggacagac
    31441 gaaaaagagt ataatagtgt tatagcagca aaaacagcag atacattgct ttcgatgatc
    31501 aatatcaatg cagctttcgt cattacaaag cggacagacg gcttgattgg gatcagtgcc
    31561 agaagtaccg gcacgatcaa cgtccaaatc atcatggaat ccttaggtgg cggaggacac
    31621 ttcacaaacg cagctactca actggaaaat acgacagttg aggaaacaaa acagaagcta
    31681 tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata aggagtaatc caaatgaaag
    31741 taattttttt acaagatgta aaaggaaaag gtaaaaaagg ggacatcaaa gaagttccag
    31801 caggttatgc acaaaattat ctaatcaaaa ataacttggc aaaagaagca acgaaaagca
    31861 gtatcagtca gttagcagga caaaagaaag cacaagaaaa acacgaagct gaaatcttgg
    31921 aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga tactgtagtc gaattgaagg
    31981 cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc ttcaaaacaa attgctgaag
    32041 ggttaaacaa acaatacaaa atcaaattag ataaacgaaa aatcgaatta gaaaatccga
    32101 tccgttcact aggctacaca aacgtacctg ttaagcttca tcatgaggtg acggctacga
    32161 tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc gatgttcagc tggtaagtat
    32221 aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa aacagctgca gcaatgaaag
    32281 caaaagctgc aaatcgattt cagattgatt tcggtttctt ttttgggaac agcaaaatag
    32341 cataaagaaa acgagtataa taaaagagaa ctattttttt attacacttt cggtacgaat
    32401 gatgactttt ctctcatttt cttgtaaaat gaagcaattg aagacaagga gcgatacgta
    32461 tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat tgaagctgaa caagcggttt
    32521 taggctctgt ttttcttgac gcagaaacga tcattgatgc catggagtac atagagccaa
    32581 aagactttta tcgtcgtggc catcaaatta tttttgagac aatgatcgag ttaaatgatc
    32641 gtaatgaagc gattgacgtg gttactgtca aagaccggct ggaacaagcc aatttgttag
    32701 aagatgccgg tggtttaagt tatctctcag acttggcatt agcagttccg acagcagcaa
    32761 atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt gctgcgtaca ttgatccaga
    32821 cagcaacagg aatcgttacg aaaggattcg agcaaggaga agaagttcaa acaattttag
    32881 atgatgccga acgcagtatc ttggaggttt cggaaaaaag aaatcgaagc ggctttttat
    32941 ctattgctga agtactgaac tccacgattg aaaatatcga tcgactagct caaaacaatg
    33001 aagaaatcac aggtctacca actggctatc aaggtctgga taaaatgaca gcaggtttcc
    33061 agcccgaaga actaatcatt cttgctgcac gtccagcggt aggtaaaaca gcgtttgcat
    33121 tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc tgttgcgata tttagtttgg
    33181 aaatgggggc agaatctctt gtaaatcgga tgctttgtgc agaaggttcg attgaggcca
    33241 gccatttacg tacaggtcag ctttcggaag aagaatggtc gaatctgatc gttgcgatgg
    33301 ggagtctttc tcgagcaaat atttttatcg atgatacacc agggatcaaa atctctgaaa
    33361 tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa tctaggcttg atattgatcg
    33421 attatttgca gttgattgaa ggtaacggta aagagaaccg tcaacaagaa gtatcagata
    33481 tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt acctgtcatt gctctttcac
    33541 agttatctcg tggagtagaa cagcgacaag acaaacgacc agtgctaagt gatattcgtg
    33601 aatccggatc aattgaacaa gatgcggaca tcgtagcctt cttgtatcga gatgattatt
    33661 atgaaagaga aggcggagat gacgatgatg gaaacgagcc aaaaaatgac aacatcatcg
    33721 aagtcatcat cgagaagaac cgaagcggtg cacgaggaac agttgaacta ctgtttatca
    33781 aagaatacaa taagttttcg tcgctttcgc caagagaaga attttaaaga agaggttgtg
    33841 agccttgagc aatcccgaga gctctcgtaa gaagatgcaa ggagtaaccg caggaacgac
    33901 tagagtactt aatttaaaca gatctccaaa gggattgtga caaaagtctt gtcacagtcc
    33961 ctttttccga ataaacggtg ttcaatcagc tgacccttgg tattagctca gcaaacgtca
    34021 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatctt
    34081 acgacctctt ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    34141 catcaaacat gagaagcagt tttttgaaat tgctccttgt gagtcagagt taaacggcag
    34201 gctcacaacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa tacgcccaat
    34261 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg atgcaggaca
    34321 gctttttcac aacctctgat tcacggtgtt ccaaaagtag cttcttcgaa aaataagtcg
    34381 aaatttcaca aaaatttgag aagcaatttt cgtacattcc ttcttatttc ttgaagctga
    34441 ccacttctgt tacaacctct tttttaataa ccattctaaa aagataaagt tcgtttttta
    34501 agcgttttta taaaataaaa ataaaaatca ttcgatttta tttgaaatat gtatctatta
    34561 ttgatacaat ggttcaggat taaataatat agaacgaggt gttcacatgt catctgtagt
    34621 agtagtaggt acgcaatggg gcgatgaagg gaaaggaaaa atcacagact ttctcagtga
    34681 aaatgctgaa gtgattgcac ggtaccaagg tggcgacaat gcagggcaca caattaagtt
    34741 taacggtgtg acttataaac tgcatttgat tccttctggg attttttata aagacaagat
    34801 cagtgtgatc ggaaacggcg ttgtagtcaa tcctaaatca ttggttaaag aattagctta
    34861 tttacatgaa aaaggtgtag aaacaagtaa tttgcgtatt tctgaccgcg cgcacgtaat
    34921 cttgccttat catatcaaat tggaccaatt acaagaagat gctaagggcg aaaataagat
    34981 tggtacaacg attaaaggaa tcggacctgc ctatatggat aaagcagctc gtgtaggtat
    35041 ccgtattgca gacttattag ataaagaaat ttttgaagaa cgtcttcgtt tgaatctaga
    35101 tgacaaaaac cgtcaatttg taaaaatgtt tgattcagaa ccacttgatt ttgacgatat
    35161 ttttgaagaa tattatgaat acggacaaca aatcaaacaa tacgtcacag atacttccgt
    35221 tatcttgaat gatgctttgg atgcaggcaa acgcgtgttg tttgaaggcg cacaaggtgt
    35281 aatgctggat atcgaccagg gaacttatcc ttttgttact tcttctaacc cagtagccgg
    35341 tggggtaaca atcggaagcg gtgtagggcc ttcaaaaatc aataaagttg tcggcgtctg
    35401 caaagcctat acgtcacgtg tcggtgatgg accatttcca actgaattat ttgatgaaac
    35461 aggagaacaa atccgcgaag taggacgaga atacggtacg acaactggtc gtccgcgtcg
    35521 tgtaggctgg tttgattctg tagttatgcg ccattctaaa cgagtttcag gtattaccaa
    35581 cctatcttta aactcgatcg atgtattaag tggattggaa acagtaaaaa tttgtacggc
    35641 ttatgaatta gatggcgaac taatctacca ctatccagca agcttgaaag agttgaaccg
    35701 ctgcaaacca atctatgaag aattgccagg ctggtcagaa gacattacag gatgcaaaac
    35761 acttgcagaa ctgccagaaa acgcacgtaa ctatgtacgt cgtatctcag agcttgttgg
    35821 tgtacgtatc tcaacatttt ctgtcggacc tgatagaaca caaacaaaca tcttagaaag
    35881 cgtctgggcg caaatctaag gataaaaacg acgactgaat caggacgttc ttaccagagc
    35941 gagtcgaagt gtttatctat acgataataa taacttgaag aggtagtgaa agagacgttt
    36001 agccttattg ccgataggct gtctcttgaa cactgtttat tcagaataag ggactgtgac
    36061 aaaaaccgtg tcacagtccc tttttccgaa caaacggtgt tcaaaagctt gcatctacgg
    36121 cactaagccc aatcagatga aaaatatgga gtgctatttt tatctgattg ttcttactgc
    36181 ttgatgcaga gcagcttttt cacaacctct ccactaaacg gtgttctcct gccaactcct
    36241 cggtcacaag tcacaatatt caacaaacat gagaagctgt tttttgaaat tgttccttgt
    36301 gactcggagc taaacggcag gctcacaacc tcttggtaaa cggtgttcaa tcagctgacc
    36361 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttttccgt ttttttctct
    36421 aatactcagg tcaaaacgct gatttcacaa cctctgattc acggtgttcc agaagtagct
    36481 tctacggaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc
    36541 ttatttcttg aagctgacca cttctgtcac aacctcttat tttgattaga acttgcgaaa
    36601 ataaaccggt taaagtgtgt ctagcgcaag agatttgcta taatagacga taagggtaaa
    36661 atgtaacgag aaggggttga aaaatgtgta tcaaattgta acagattctt gttgtgatct
    36721 gccataccat gtattagcaa aagaaaaagt tgatttttta tcaatgaatc tccaattgaa
    36781 tgatcaagag ttagtagatg acctcggaaa gacattcaat tatgaccgct acatgaatca
    36841 gctgaaaaat ggtgcgatgc ctacgacatc acaaatcaat gttggccgat atttagaatt
    36901 tttccgttct tatgcggaaa aacagattcc tgttttatat gtggcttttt ccagtggatt
    36961 aagcggctct tacagtagtg cgcttcaagc ggttgaattg ctgaaggaag aatatgaaga
    37021 ccctgagatc tatgtttttg atacaaaagc tgcaagtatt ggggaagggc ttttagtatt
    37081 ggaagctgta agattgaaaa aagaaggtaa aagcatcgaa gaaaccctag agtggctgga
    37141 aagtaattat ttgaaagtcc attcatgggt aacagtagac gatctgaaat atctagaacg
    37201 aggcggtagg atttccaaag caagtgctac tcttggcagt ctattgagtg taaagcctat
    37261 tattacggtg gataaagaag gaaggctgac gaatgttgac aaagttcgag gaagaaacaa
    37321 gtcgatccag acaatcgtca atgagacggt aaaaacaatc gttgcaccac ttgatcaaat
    37381 cgtttatgtg gcttacgctg gggatctaga gagcgcggag aaggcaaaac ggcttttgga
    37441 agagcagata aagatgaaag atgtgaagct ttatccctta ggaccaacga tcgccagtca
    37501 cacaggctat ggctgtatcg cgattttttc gatgggaatc agtcgataaa agcagtaact
    37561 cattctgtaa aaaagcccag aaattgattg taacaaaaac agcgactctt aaaagataga
    37621 tgagcatcct atctcttaga ggtcgctgtt tttttatttt ttaagctaaa taggccatta
    37681 tgcccataac tcgttcaatt gtttttgaat atctgcattt tccaaaaatt catcatatgt
    37741 cgtatcagct cgatcgatca cacctttatc cgaaacggca atgatgcggt ttgccaatgt
    37801 ttggataaat tgatggtcat gtgacgcaaa taagattgag ccagtaaatg ccatcaagcc
    37861 gtcattcaat gctgtgattg attctaagtc taaatggttc gttggatcat ctaatacaag
    37921 tacgttagct ttcgaaagca tcatttttga aagcatgcaa cggactttct ctccaccaga
    37981 taaaacattc actggtttca atacttcttc accagaaaac aacattcttc ctaagaagct
    38041 tcttaagaag gtgttatcat cttcttcttt acttgcatat tgacgcaacc agtctaaaat
    38101 cgttagatca gtatcgaatt ctttcgttgt atcctttggt aaataagaat tacttgttgt
    38161 cacaccccaa cgtactgttc cagtatctgg cgtaatttca cccatgataa ctttgaatag
    38221 cgttgttgtt gttatatcat tttctgcgac gaaagcgacc ttgtcttctt tgctcaaatt
    38281 aaaagatata tcatctaaaa ttttctttcc atcaatcgtt acacttacat tttctacctg
    38341 caataagtcg tttccaatct cacgttcagg tttgaatcca acgaatggat aacgtctaga
    38401 agaaggttgg atatcgtcta aagtaatttt ttcaagcatt tttttacgtg aagttgcttg
    38461 ttttgatttt gatgcattcg cactaaagcg ggcaataaat tcttggagtt ctttgatttg
    38521 ttcttctttt ttggcatttg cgtttgcttg aagtttagcc gctagttgac tagattccaa
    38581 ccaaaaatca taatttccta cataaagttt gattttacca aaatctagat ccgccatatg
    38641 tgtacatact ttgttcaaaa agtgacggtc atgggataca acgatcacag tattttcaaa
    38701 attgatcaaa aattcttcca gccaattgat cgattggata tccagaccgt ttgttggttc
    38761 gtctagcaaa agaacatcag gtttaccaaa tagtgtttgt gctaataaca cttttacctt
    38821 ttgtccacca gttaattcac tcatttgctg atggtgcaat tcttcaggga tgttcagacc
    38881 ttgcagtaaa acagctgctt ctggttcagc ttcccatcca ttaagctcag caaattctcc
    38941 ttctaattca gctgctcgaa taccatcttc gtcagtgaaa tcctctttca tgtaaatcgc
    39001 atctttttct ttcagcactt catacagacg cttgtgtccc atgatgactg tttcaagtac
    39061 agtatagtct tcatagtcgt aatggttctg tttcaatacg gccatccgtt catctggtcc
    39121 catagtaacg gaacctgtgg aaggctgcag atcccctgat aacactttaa ggaacgttga
    39181 tttacctgca ccatttgcac caatcaagcc atagcagttt ccgggggtaa atttaatatt
    39241 cacatcatca aatagtttgc ggtctgaaaa gtgcaaactc acatcattta cagtaatcaa
    39301 tttttttcct cacttttatc attattagtt ttgacaagtc ttatagaatt atatagggga
    39361 aaggtaaaag attcaaggca atcgaacatt ctttgtgttt cacgtgaaac agaatgaaaa
    39421 aaataaaaat aaaagcggag cgaaaatagc tactatagga tttattagtt tgcaacagca
    39481 taatttttga cgttatttat aagagggagt actataaaac tgtaaaagaa atgaaagaag
    39541 gattgaaaat ggatgaaaaa agaaccatcg actggggctc attgattttg ggaattttat
    39601 ttgtacttgt ctcattgtta tcatttcaag accctgtcgg aaatttagtt gcaattgtag
    39661 tagtctttgc gatttttgcc tttattaaag ggatatttga actgtttgta cggaacagat
    39721 taaaagagct aacaggatat aaaggaaaga tgcccttaat aatcgggatc atcgatattc
    39781 tagttggcgt ctttttctta ttcaatattg gtgcaggagt cgcagcattg ccttttgttt
    39841 ttgcagtatg gtttattgct gattcagttt tagcgttatt tactgctgac ttggcaagag
    39901 gagtaagtga aggatattat tggtttacga tcatcgtcaa tattttagga attttattag
    39961 gaattttctt attgtttaat ccaatttctt ctgcgttgac attaagcttt ttagttggat
    40021 tctattttat gttatttggt atcacacata tcgtctatgc atttagataa gtaaaatcaa
    40081 acaaataaaa aagccggctt ccgattacag ttttactgtt gtaattggaa gccggctttt
    40141 ttatcatgga caaatatgca cgaggtgatt tttaggtgtc gagacaaatt tatgcaaaaa
    40201 tgtgagaagc tatatacaca aattttatct taatactcga agcatagcgc agttgtcaca
    40261 acctcttttc atataattca ttcaatactg gcaaatcatg gacttcatat ttttctttga
    40321 atgcttggat ttcttcttca gaatattttc ttggatctaa atgcagatct tctactggca
    40381 atgggcgctt gttattgatc ttcacatcga ttactacagg acgatctgat tttgcagctg
    40441 catcaaaggc aaatcgaagt tgcgagtatt ctgtgactgt aaagctattt gccccgagcg
    40501 cttctccaac tttaccgaaa tctgcatctt ctaaaaatac accaaatttc ttttgttccg
    40561 tatcttcttg ttctgcttcg atgaaaccaa aagaatcatt cgaaaagaca acattaataa
    40621 ttggaagctg atatttgact tgtgtaatga tatcttgcat gaccatagca aacccgccat
    40681 caccacttaa ggtaaatact tgtttttctg ggtagcttag ttgtgcggca attcctcctg
    40741 gcacaccatt acccatagtt gcgaaccatc cagatgtcgt atgtttttgt ttaccgttca
    40801 tattcaataa acgaatagaa tggatagtcg tgttaccgac atctgtgacg aaaatcgcat
    40861 cgtcttcagc aatacgattg atttctttat agacagcctc tggacgaatc ggttcttcct
    40921 ctcgatcttc aaaagatttc aaccaattta cccatttttc tttattttct tgatttgcta
    40981 ggtaccagct atcagcaggg cgagcatccc ctaactcgac aagtctttgc aaagctgtgt
    41041 ttgcatcacc gaggatcgac aaagatgttt catggcgtcg tccaaatttt gacgcatcga
    41101 tatcgatttg gataaattca gcagttggat taaaaaatgc acgtccaaat gggaagtcgc
    41161 taccgacaaa taagatcaga tcggcttctg ccaatgcttc atttccgggt ttagtagcga
    41221 cacgtccagc aaaaccaaga aagttttcat aatcatcagg aataattcct tttgctagaa
    41281 cggaagcgac aacaggaact gaaaaatgtt ctgagaattc tttgatttgc ggaaatccgt
    41341 ttcgcgttcc ttgaccaaca taaagaactg gtttttttgc tttttcaaga taaggcaaag
    41401 cagctagcaa atctttttct tctggtaata tcacacttgt tttgtgagtg tgagcggtgg
    41461 agaaaggttg ttcgtcaatt tcttcaaaac caagatcaac agggatagtc acgatagcca
    41521 cgcctttatg ctcataggcg gctttaatgg cttcatctac tacatgcgga agactttgtg
    41581 gtgtcatgac tgtacgattg tatacgctaa catctgcgaa aataggattt tcatttagtt
    41641 cctggaaaga gttatagttc atggatgtag aagcaacttg tcctaagaac gctactagag
    41701 gcacatggtc catctgtgca tcatacaaac cattgatcaa atgtgtcgca ccaggtcctg
    41761 cagagccgaa gacagcgccg actttccccg ttaattttgc atctgcagcg gctgcgagtg
    41821 cgccgacttc ttcatgacga acttgaatgt agttgatttc ggttttttct tgatatagcg
    41881 catccataat tgaattgaaa gatcctccgg gaatgccgta aatatggtcg ataccccagc
    41941 tttcaagaac ttttaccatt gcgattcctg cattgatttt tgacatggtg tttcactcct
    42001 ttagcaatga tgatacataa attttttgta tctagagtat agaacttttt atcgaaaatc
    42061 ccaactaatt cgtcttttca caaaaatgta gtcatgtttt tgggaatatg ctagagtagt
    42121 acatagatag attcaagaga ggggaatggc atggttgaat ggctattgag acttgaacct
    42181 tggcagcaag cacttgctgg gacagctttt acttatttta tgacagctct tggtgccggg
    42241 ttggtttttt tctttaaaga aatcaaaaaa gaagtgctaa acttgatgtt aggatttgct
    42301 tctggtgtca tgattgctgc aagtttttgg tctttgttag atccggctat tacaaaagca
    42361 gaagaaaatg gagatattgc ttggctagtt gtaagtattg ggtttggttt aggcggtgtg
    42421 tttttatata tggcagataa aacactgccc catatgcatt ttggaccaca acatgaagca
    42481 gaaggattgc ctacccattt aaaacggacg atcttgcttg ttttttctat tacattgcat
    42541 aacattcctg aaggtttagc cgtgggggta gcattcggtg cagcagcaac tgcggataat
    42601 ccgacagccg ctatattagc cgctgtttcg gtggcgctag gtattggtat ccaaaacttc
    42661 ccagaaggag cagctgtatc aatcccgttg cgacaagaag gtctgagtcg taaaaaggcc
    42721 tttgtttacg gacaagcttc tggaatcgtt gaaccaattg ctggagtgat tggagcgcta
    42781 ctagtaacga aagtggagct gctgcttcct tatgcacttg cttttgccgc aggagcaatg
    42841 atttatgtcg tggtggaaga attgatccct gaagcacagc agacattgtc cagcaaacgt
    42901 cattttgcag tttttggcgt catgagtgga tttatcatta tgatgatttt ggatgtggca
    42961 ttaggataaa taaaaaaaat atatatgata tatcaaatag ggccgcgaac ttatatcgcg
    43021 accctatttt tttagtaagt tatatcataa tgaagtgaat ggtatcatac attaataata
    43081 gttttttcta tgtgtatttc ctttgatcca gcgaacaagc cagatgataa gtgcgattgg
    43141 gattagaact ttgaccgcaa accataaaag cccgcttagg acactgacaa aaagactgat
    43201 gacgaagcta gctgcaatta gcactaaaag aaccatgaag atatttaaga acatattttt
    43261 ttgacgcata caaaaccctc ctcttgttca ttctttacta tagaaaaagt ataacaggga
    43321 ttgtttaaaa acacctccaa ctgaagactg atttttactt catctactta aatgatcaaa
    43381 caattgcttt ttctctggtt tatcatctaa aatagagcgg aaaaggagga gaatatcatg
    43441 cctaaaaatc gtcttgaagc atttacagat gctgtaatcg cgattatcat gactatttta
    43501 gttttagaac ttcatgaacc taacggagac acgtgggagg cgctcgctgc attaggttac
    43561 cgatttttta tctatattgt cagttttatc attttagcga tttattggaa caatcatcat
    43621 catttatttc aagttgtaca taagataaat ggacgggtct tatgggcgaa cagtttcttt
    43681 gtttttgcgc tttcgctttt tccgtttgca acttcatgga tcagtgagta catcaatagc
    43741 ctggctccag aaatgactta tggttttgtt accttgaccg ctaatattgc gtattatatc
    43801 ttggcaaaag aacttgtcaa agcaaataaa ggccacgaga aagcattgaa ggcgatgttg
    43861 aataattaca agaaatcgta tctatcgatt ggtttgaatg tgttaggaat tgtcttaggg
    43921 tatttagtag cgccagtagc cgtgctgatc gcaaacatcg tgattctagc tgcttggatc
    43981 atcccaagtc gtaccatcga atcacaatat cggtaaaatg atccgtgttt aaaatttaag
    44041 agaaaaatat agaaaaaata aataaacgat caaatatccg agtgatacta gtattcaaag
    44101 agggatgcag atggatcaaa aaagaattca tgtaggactg attactagtt ttgtcacgat
    44161 gcttatttat ttggatcaag gtcatttgac ttggacagta gttggtgtcg taggattttt
    44221 gacattattc acagcaataa aaaaataaac atctttatgt aatctgatgt tctatctatt
    44281 taagaaaagc atgtttacag cagtatgatt ctgttgtaaa cgtgttttta tttgtggaaa
    44341 atttaatttt tagaaaagtg tatcgaaaaa tagatagtta gtttttccac agtggataac
    44401 ttcgtaagag ataaaaataa aaaattagtc aaagaagggc gtatagattt cttttgatag
    44461 gagatataca acagaaatga ataaatatta acgtgttttc aaacagtata aaacggtgtg
    44521 gtttttggaa taaaaaaata acatatctca ttttacactt ttgttatttt atacttaaaa
    44581 aataacgaca cagaaaatac cgtgtctcag atttctctac tacttctaaa aactactgtt
    44641 tttttccttt gaaatgcctt caaaaagtag gatcaggcag aatatactgg cagaatcaag
    44701 gagaagggag cctaaataag gagaaaaacc atagaaaata taaagtaatt acttataaaa
    44761 caagcttttt atagaaaaat aatatacctg agtagggtat aaattaagac ggaattcctt
    44821 tctagattga gatattgact aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa
    44881 aacccttttt gttaaataag gggaatatag cctgtcagtt tcaaactgca agtcgtcctg
    44941 ctgcaataat atagtagatt agagatgaaa atagattttt ttaaaccaat gactgaataa
    45001 aaagaccgtc atagatacac attggaatgt gtctattacg gtcttttttt acgaacgatt
    45061 ttattgaaga cggacagttc gggtcaaaaa taaaaaagct acgatcagaa ataagaataa
    45121 aatgtatccc aaaataatgg ctagacggtc taacttattt gtcgatagat gtttgtttag
    45181 aagttgatag gcccataaac ctatcagtcc tcctgttgta ttagttatca aatcagtgat
    45241 gtccgctacg ccaataccga ataggaattg aatcaattct gccaagaagc ttacaaggaa
    45301 aataatccct aattttttag aaaaagaaag tctcttgaaa ttgacattca aaagcacccc
    45361 aaaaggaaaa aagacaatca agttataagt ggtctcacgt aataccgtcg tattttcaaa
    45421 agaaaaagta gaaaaaggga cgagattgac attgctgtaa gaataagtga gaaattgtgg
    45481 aagattaaat gatagcttga ataaaatcaa ccataataga aagaataaat aaatccctaa
    45541 taaaaatttt gatacatacc gcatagacat ctcctccatt taaaaagtat accatcgaaa
    45601 accgctgaaa aatcataaaa aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa
    45661 attagaagct ttttttgttg ggaatccggt ttctttaaca aagctaagat agcgagttgc
    45721 ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta
    45781 atttctccaa gttgtttact ttttgatact aagtttatga aaaatcaaga aatagttgtt
    45841 atactaggta atgctgacaa acgggattgg catttcatct tggttcagct ttttttccaa
    45901 ttactaggaa ggagggatag attgttcaag acttattttc gtttcaataa aatacaagat
    45961 aatccttttc gtatcatgaa tctgatgcta cttatcttta ttgtattatg gttaggttac
    46021 ataaatcatg attttttgat ttcaagtttt tcatcattag ggatctttac ttttttctat
    46081 tatcaaaaca taccgatgaa atatttgctg aaacgtatgt ttctgatcgg ttgcggactg
    46141 ttggttgctt tcgttttggg gatcttatcg acttatgttt tatggcttga accatttgcc
    46201 gtagctttgg ttgctttttc cagcagattc gtgttgcgat tattccatat ttctaaacca
    46261 ggtggcttgt tttttgcgat gctttctgcc atgggaacga gcatgcagct gccaatcgct
    46321 caattaccga tcgtctcact ttattttttt atgggtgtcg tttttgccct gattgcggca
    46381 gtgattacaa aattactgga ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa
    46441 gaaagatttc acgaagagcc tttagtgatc attgattctg ttttctacag cgctgcctta
    46501 tttctttccg tttatgtcag tcacggcttg aatctgcata atccttattg gttgaccttg
    46561 tcatgtgcat cgattttgct agcagaaaac ttggatgcga tgaagcatcg acaagtccaa
    46621 tatctgattg gatcgatggg tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt
    46681 acgcagttgc agaccatttt tctgatcact tttctatatg gaatcgcaca atttctagtt
    46741 gctagaaatt atgcagttgc taatattttt cttaatccga tggcgttgat gctatctaca
    46801 cttattcgtg gagcatactt gatttctttg atcgaatatc gttttttagg gatcgtcata
    46861 ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg ttggtttgca acattattta
    46921 acggttgttc gtcaaaaatt agaatagaag gacaaatata tgtgccaaaa gaggttatga
    46981 caagattttt gtcacaacct ctaattcaag gtgttcaaaa gccagattct cagcattaag
    47041 tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa attttatatt tattctatca
    47101 aaaaattttg tttatgatgg taactgaata aactttcctg agatggtcaa tgctttcctt
    47161 gaaagtctta cagatattct ttcttcatag aaagtttaag ttttatagtt agtgtacaat
    47221 aaaagtatgt ttataccata tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa
    47281 agatatgaaa aggggaaatt tgatgaagaa aataagcaat gaaatccaaa ttctggcaga
    47341 aactttaact cttcccaaag tcgttataga gcaaatgatt gtacaagcaa aaaacatcct
    47401 tgatgaggtg tttgagcaat atagtcatga actaatggat cctaataaag caaaggcgac
    47461 tgcaaagaaa atagacaaag tctatgaaga ggaacctttt gcagcattag ctatttatct
    47521 atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc caagaactat
    47581 ctatttggca acaatgaatg cttttactag atttattcat gaacatttcc aagtaacagg
    47641 aagctatcgc tttgaccgtg gtttttggat ttggcattac actagtggct tgatctttcg
    47701 gattggtgaa ttggagtacg aaatgacata ttttccgaaa gggtacacgg atctaagatt
    47761 agaaggaaag cagtgccttt ctcttcatat tccttccgat gcagatttat ctcagaataa
    47821 gataagagat agttacgaaa gtgccattcg tttttttgaa ctttattttc ctgattacca
    47881 ttaccaagtg atgtacacag atacttggtt actatcaccc aatcttacta aatggttgaa
    47941 aaaagagtca aaaatctgtc tttttgcagc cgattatcga ttattatcag ttgatgaaca
    48001 ggacgatagt ggagtccctt ggatctttgg acgagtagat gcccaaatac atgattatcc
    48061 agaaagtacg agtttacagc gacaagcaaa agagcaattg cttgcaggcg agcacattgg
    48121 ctctggctta ggtattctta cttcataagt ttagtacttc gaaatagttt aaaagagcgt
    48181 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    48241 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
    48301 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt agtgttctat
    48361 cgaacacaca gtacgttcaa taggtaaact agctataaca atagatatta aagttaacgg
    48421 acaagagggc cttttttagg tagctaaaac gtaataatga aaatttattt ccagagatag
    48481 gagtcaatca gctagtctga tcgactgttt taacaagttg actttcattt taacttttga
    48541 taccatttaa aacacaaatc gtaattgttt cgatttgtgt tttttagcat gactacaatc
    48601 aaagaagaaa gtgggtcgtc catatggaaa aaacagatat ctcaagtgca taccgccgct
    48661 tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac
    48721 ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt
    48781 tttcggctat tttcaataat cggtttttgt gtaaggtttc cgaaagaact gctgttgatt
    48841 ttccattttt aacatatgat aaattggtag aatctttctt ttcaaattga taccattccc
    48901 taattccagt tatatagtta actacagaac cacaaagcac gtacgtttat aaaagtatta
    48961 tctgtagtta tttttcaatt ccctttcaac ctcttatttt gaccagtagt attgatcact
    49021 ttacctttta acacctttgt tcgtttttga aacattttta gtcaagaatg gaaacgtaac
    49081 tacataatca aaggttttgc gaaatatttc atgaaatatt tcatgtagaa gcgagcaata
    49141 cggttgcaaa taaccattat cgtttaataa gcagttacga aagctcccaa tatctctaac
    49201 gttcagctca ctccatttct tttcttcaaa aaaatcagtg attcgtatct ctttctattt
    49261 gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca
    49321 gttactattg ctagaataac atcaaggaat aataagtttt ggttttcaga taatagtcga
    49381 taagttgtgt attggaattt tagatacagt aataaagtat ttttcatgaa attttgtgag
    49441 aatcactatt acaagtatct ttaataaaat taatggttaa ggaggaactt aaagtagaat
    49501 gtaaatgaat agttgtagtc agtcgattgt gatactagac tcaaagttgc aggaataagt
    49561 ttttcaattt gtgattggaa gataaaaaat gaaaaagaag ggcttttttt tatactttgt
    49621 tgattgtagt gtttattttc cctctgttta gccaagtaac attttctatg actcaaccag
    49681 tttcaagtga ggaacatgat aacctttttc gtgctacaga agagtttaag aaatatcagg
    49741 aaaatattgt tccggagact ccattagcta gtgagccaat tactgatatt gatggaaacc
    49801 aaataggatg gtatatacag tacgaagtta tggtggtaaa taatgactca atagtaaaaa
    49861 atacagtagg cttagagcta acaccgattg aaaaggtgag ttcattgtta acgtatattt
    49921 atactgatga aagtgacgag actgagatca ttttaacgga ttactcaaaa attgctactg
    49981 ataaaaagta tatattgtgg atgtagacac tagtgaaata ctttatgata tagaaattga
    50041 tgaaaattta ctagatgtag cacaagaaat cgatcatcaa aagcaggaag ctattcgata
    50101 tgctggtaga aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa gttagagaag
    50161 atgaaaatga gtggaaagaa ttcaagagtc gctactcaat taactcgact ccgacattta
    50221 cagtttacag agaaggatcg atagaaaaaa ctgttttttg gactaaagaa agtggaatgt
    50281 ctttagctga agtagaagag tttttggatt atgttagtat gcaacagtaa gggagttctt
    50341 attatgaaaa attttgacac atattatgtt attgtttttt cgatatcttt gctgttaata
    50401 ttactaactt taatgcagta taaagagtat ccagagtatt ttattttaac ttttgtatat
    50461 ttagtaagta gctacacact tctatataaa ttcagaagtt cttctagagt ttctaaagtt
    50521 tgtttcttaa ttagcttatt gattctataa tatttttgat ttatatagga aattaaaaat
    50581 attatagaat caacactcag agaacgggtt ggtttttaat agatttaata agtactgata
    50641 atgaaatttc tcctggttgg gaaaacgaag ttggttaccc tgtagaagaa ttttttcaag
    50701 attactttgc ggagtatgcc gataggataa tgagagatta cgtaacagat aaaactcaag
    50761 aagtcatcaa tcaatcccag tcattaaatg aattatctga taaggcgaaa gaagaagcag
    50821 ttgccattat ttcttcaggg attagtgact atattgatat gagtggtgaa atcaattatg
    50881 gcgataaaga caaaattgta cgcatgataa aagcttttat caaaactttt gatgtgaagg
    50941 cagatcttaa gtttaatgat acttacctag taggaaaatt aattaatatc cttgggacag
    51001 agaaagatac ggaaacgttt atttcctctt tgtctatgtt gtttacagat aagcattttt
    51061 caactcagct tggtggagag gaactagtgg aaatctttta accattacgc ccagcattaa
    51121 taaaagtata tcaaaaaagt actcctgatg atttttaaga ttggtttgag aaataatgtg
    51181 aaaacactat ttttaatgtt aattatagat agagatgaac gcgtcatatg gttaaaagaa
    51241 tgaaaaagtc aaagatacac tgctatattt cactaaggat atagcagtgt atctttcatg
    51301 tcgtttcact ggcagttttt tataaaagga ggtgtgcatt tcagttgctt ttctttggta
    51361 gttagttgaa tcgtttaaga attaacggat gtggcagaca ggtgctctgt ccaaaaacca
    51421 gtaaaatctt tgatggttcc atctttattt agctgtgcct gttcaaatac tgttttgttt
    51481 ttaccgaatg gatcaattgc aataattgtt ccgatccccg cacgtttggc agcaagtaat
    51541 cctgagtaag catcttcgat aacaagacaa tcagccggag ctaagctcag tttgtctgcc
    51601 gctttaagaa aaatatccgg ttgcggtttc ccagggaaag agccgtcgtc atagacaatc
    51661 ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa aataaaaagc aacattctct
    51721 ttgacagtag ccgtggcaat cgttaatgga atctgttggt cagtcagctg gtcaagagtc
    51781 gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt gcaagcaaag catacgataa
    51841 tagctttctt tttcttctga caatacggcg atctcagagg cggtcaaatg ctctgaaatg
    51901 aaatgtttca aaatctccgt attcgtccga ccatgaatat tttttaaaat atcctcgtct
    51961 gataaaccat cttttgtatg atcatgtatc atatgcagcc aagcagcttc atgcagatga
    52021 gagtccaaaa acattgttcc gttgaaatca aatataatcc ctttcatagt tacgctccta
    52081 tagtggtatt ttcggttgaa tcagcaattt gcagatcgac atattgtgca tagtgctctt
    52141 gaacctcccg cgagatctgg cgatccgtaa tgagcagatc gaaatcatgc aaattataaa
    52201 acacataaaa atcatctcga ttgaattttt tattatcaac gagtagatat ttcttacggg
    52261 aattatttag agcgatctgc tgtgcttctc cttcttcggt actgtaagta gagatcgctt
    52321 cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa ctttagtctt cttaaattgt
    52381 cgttggtgat cggtccgaca aatgcgccgg tatttttccg gaaatcaccg ccaatcagta
    52441 gaatttctgg tccatctgct tgagcaagaa tatcaaaaac tggataatta tttgtaatga
    52501 tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc gattgttgta cctggtccta
    52561 agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt ggcagcaatt tctttttttt
    52621 cttgaatctg cagagtggct ttttcagtgt gagaaagttc ttgatctaaa gtgtaggaaa
    52681 gactttgagc ccctccatgg acacgcagca gcttgcccga tttttctaat tcatctaaat
    52741 ctctgcgaac ggtcatatca gaaacatcta attgattgat gatttcatta acggtaagga
    52801 ttcctttttt attgaccatt tctacgatag taagcaatct ttcttttttc aacattgtct
    52861 tcacctctaa tttaaggata atctatcata taaacggaca acagtcaatc taattcgaac
    52921 aaaagtaaac atttagggtg gtttttatgt ttgttacaat agattttatt gctgttttct
    52981 ggtttttatg attatttgtg tttgtttttg ttgacaaatg tttcggatgc gtttacaatg
    53041 caatcagtta gaaagagcga aaactgatta tagatttgag gagagatgta catgcttaaa
    53101 ttaccagaag attttatttt tggcggagcc acagcagcct atcaggttga aggagccact
    53161 aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt tagaggaaca agggcgattc
    53221 agtcccgatc cggcaagtga tttttatcat caatatgcaa aggacattga attgtgtgaa
    53281 cgttttggtg taaatggtct aagattatcc attgcttgga gccgcatttt tcctgatgga
    53341 gctgggaaac caaatccaga aggaatcgct ttttatcatc gtgtgttcga agaatgtaag
    53401 aaaagaaacg taacgccgtt tgtgacgttg catcattttg atacccctaa acgtttgttt
    53461 gaccatggag attttttgaa tcgagaaaca atagaagcat ttgtttccta tgccattttt
    53521 tgttttcatg aattcaaaga agtcaaggtg tggagtacct ttaatgaaat ctatccagtt
    53581 gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa tcaaatatga ttttacaaag
    53641 atcgtcgcct gtcttcataa tatgatggtt gctcatgccc gtgtagtgaa ctatttcaaa
    53701 gaaaacgaac ttccgggtga aattggtgta gttcactcat tagagacgaa atatgcggca
    53761 acagacgcgc cggaagataa gcatgctgcc tttttagacg atgcattgtc gatccgtttc
    53821 ttattagatg ctacctattt aggctattat tcaactgaaa ccttgactgc tcttgatgag
    53881 atatgtgaag caaatcaggc cagctatcat tttcctgaag aagatttcgt tgaattgaaa
    53941 aaagcatcca cgcgtaatga ctacttaggg atcaatcact accaatgcca ttttgtcaaa
    54001 gcttacgatg gagaaaatgc aatccatcat aacggtacgg gtgaaaaagg gacctctgtt
    54061 tataaagtga agggaatcgg agaacgtatt tataaagaag gaattcctcg aactgattgg
    54121 gactggctga tttatccaga aggtctatat gacttgttgc tgcgaattaa aagcgattac
    54181 ccacattaca acaaaatcta tatcactgag aacgggatgg ggtataaaga tcagtttgaa
    54241 gatggcatta ttatggatca gccgagaatc gactatttga gagtctattt ggaatcctta
    54301 agcaaggcga taacagccgg ggtaaatgtg aaggggtatt ttttgtggtc gcttatggat
    54361 cttttctcat ggacaaatgg gtataacaaa aggtatggct tgttttatgt tgattttgaa
    54421 acccagaagc gttacccaaa agaatcagct tactggtaca agttggtcag tgaaaccaaa
    54481 acgattattt aataagtgat aatggaggaa gaaaagatga ataaagagga attacaaatg
    54541 cttggttttg agatcgttgc atattcaggc gacgctcgca gtacattatt gaaattattg
    54601 aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg cgttgaaaga agccgacgaa
    54661 aatttaaccc ttgcacataa ttcgcaaaca aaaattttag cggaagaagc atctgggaaa
    54721 gagatggaga tgggattcat ttttatccat gggcaggatc atttaatgac cactttattg
    54781 ctgagagatc tgattcaaga ctttattgta ctgtacagac agaatcaagg ataggagaag
    54841 aacgctatgg atggaatcat taaacaaatc gaaaaaggga aaccattttt tgaaaaaatc
    54901 tcacgtaata gttatttagg agccgttcgt gacggatttt taacagcgat gccagctatt
    54961 ttattttcaa gcattttcat tttgattgct gcggttcctg agatttttgg ttaccaatgg
    55021 cctgaatctg tctccacatg gctatggaaa gtctataatt attcgatggg tgttgtaggg
    55081 ttactcgttt cagcaaccac tgctcgctgc ttggctgatt cgatgaatcg tcgcttaccg
    55141 aaaaacaaag taatcaacac cacctctgtg atgctggcct ctgtggtaag tttcctgttg
    55201 ttaagcgtct ctccgattga aggtggattt tcaacggaat atatggggac aaagggacta
    55261 ctggcttcct ttgtcgctgc atttttaaca gttaatttat ataagttttg tgtgctgaaa
    55321 gatattacca ttaaaatgcc taaagaagtt cctggaacga tttcgcaaac cttccgagat
    55381 atttttcctt tctctttttc agtatttgcg gccgttttaa ttgatttagg cattcgacaa
    55441 atcttccaaa tctcatttgc agaagcaatc attacggtgc tgcagccgct atttacagca
    55501 gctgacgggt acttaggaat cgctattatc tggggagcga tggcgctgtt ttggtttgtc
    55561 ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg caattatcta tgccaatgtg
    55621 gaaaccaatc tgcaattgtt caattccggt gaacaagctt ctaaagtact gacggtgggt
    55681 ttagggaatt ttgtaggaac tatgggagga actggggcaa cattggttgt tccttttatc
    55741 ttcctactct ttgctaagtc taagcagttg aaggcagttg ggaaagcttc ctttattcca
    55801 gtaagtttcg ctgtcaacga accattgcta tttgcggcac ccatgatttt gaacccttat
    55861 ttttttattc catttttgtt aacacctgtt gtaaacgttt gcctgttcaa gttttttgtc
    55921 gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc agcaccgatc
    55981 ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc ggttctaatc
    56041 gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt cttagtagag
    56101 aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc cgatgtacgt
    56161 gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga aatcaatgtg
    56221 ttggtacttt gtgctggcgc agggacaagt gcgatgttag ctaatgcttt aaaaaaaggt
    56281 gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc tcattacgaa
    56341 atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta ctatgatgat
    56401 atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg agcagaatat
    56461 atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca atttcaataa
    56521 aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat ttgcagctgt
    56581 gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg aatttattcg
    56641 cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat attatgaaga
    56701 cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact atgttcgact
    56761 gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag ggacaaatga
    56821 tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta aggtgctgct
    56881 ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc ctaaagcatg
    56941 gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa agaacgttct
    57001 gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca atgaactgaa
    57061 tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag aatttgatcg
    57121 attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac ctaaaacaac
    57181 tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt ggtggtttga
    57241 tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt atccgtattg
    57301 gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac ggtatggtaa
    57361 agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg atactatgcc
    57421 aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac cgacaggaca
    57481 agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata atcgcggggc
    57541 gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg caacacaagc
    57601 aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca accaagcgat
    57661 gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag caggggcgca
    57721 aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca gtccttccaa
    57781 ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg aagcgggcac
    57841 tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg catcagctta
    57901 tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg taatgtctgg
    57961 gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg aactaaatac
    58021 tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc caattacaac
    58081 aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga cgaacgttga
    58141 ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat cggaggttcc
    58201 cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc ctttgccaaa
    58261 aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc agacttcatc
    58321 tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat gaaaaaattg
    58381 caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta gaattccgtc
    58441 gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac agttcgtttt
    58501 tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata gtctcttttg
    58561 gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa gaatgttgat
    58621 ttatagggat tccccctcca tattatgttg gctctttatt cccactccac tgttgctggt
    58681 ggtttagaag tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt
    58741 acagagattt tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg
    58801 attgaagtta cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca
    58861 ccgacagagc ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca
    58921 gcagctgcga tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct
    58981 tcagtgattt cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa
    59041 acgattgaat caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat
    59101 gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg
    59161 tgagatttga tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt
    59221 ccttgtgcta ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa
    59281 acatatacga attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc
    59341 aacttgtcta agaagcgatc tcttgcattg actttgatga tgttcaagcc gaattttccg
    59401 cctagacttt ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag
    59461 atagatgtca attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca
    59521 acgccacctg aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct
    59581 acttgcatat cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag
    59641 gcaaagtggc gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg
    59701 ataccataaa aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt
    59761 ccgactgttt caaagccttc aggaacttta gttaccaagt caccatggct catccatact
    59821 gtttgttgtt ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag
    59881 gctttcccat attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc
    59941 aattgcatac catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg
    60001 atcccaaatg cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt
    60061 ggattcatcg cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact
    60121 ccaagatcac gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg
    60181 ataatctttt caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct
    60241 ttaaatttta tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat
    60301 cgaatcgatg atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg
    60361 caagatatat tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt
    60421 aaaggcttct tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa
    60481 agccgtatcc agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc
    60541 ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg
    60601 taattgaagc gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta
    60661 cttgtctgga atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg
    60721 accagatttt acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa
    60781 tcctttacgg tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc
    60841 agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag
    60901 tctggctgtt gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg
    60961 tacttctacg tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt
    61021 catataaaga taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga
    61081 aatgcggtca ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc
    61141 attacgataa aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt
    61201 catattagcc tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa
    61261 ccccaccact taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg
    61321 gagtcttatt cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa
    61381 atgagaagga tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac
    61441 gattttgaac aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct
    61501 ggtgtatttt cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat
    61561 tgggaagagt tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt
    61621 gggttagctt tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga
    61681 gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat
    61741 caatcgaata tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg
    61801 ccgatccgtg ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg
    61861 aagaagggcg gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa
    61921 aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat
    61981 atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa
    62041 ctgggcgtgc atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca
    62101 aagaactatt ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa
    62161 aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt
    62221 tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt ggtacactga
    62281 tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga
    62341 aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt
    62401 tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga
    62461 ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga
    62521 tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt
    62581 gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt
    62641 acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc
    62701 tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg
    62761 cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca
    62821 agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac
    62881 tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg
    62941 cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac
    63001 tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc
    63061 catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat
    63121 ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac
    63181 agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat
    63241 cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga
    63301 gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac
    63361 aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt
    63421 tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg
    63481 gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc
    63541 gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca
    63601 attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat
    63661 tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca
    63721 ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg
    63781 acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta
    63841 gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat
    63901 gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg
    63961 tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt
    64021 atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac
    64081 ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt
    64141 gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc
    64201 gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt
    64261 ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc
    64321 aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa
    64381 gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa
    64441 catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga
    64501 gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg
    64561 cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt
    64621 ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc
    64681 acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca
    64741 acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag
    64801 aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac
    64861 gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct
    64921 gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta
    64981 gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt
    65041 gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt
    65101 gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca
    65161 gcaaaagatc gtgcgcttgt caaagttatt gttgaaacat cgttattaga tagagaagaa
    65221 atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact
    65281 ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga
    65341 ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg
    65401 atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca
    65461 actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat
    65521 ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg
    65581 taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg
    65641 catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag
    65701 gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt
    65761 gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag
    65821 taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta
    65881 ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat
    65941 tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc
    66001 actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg
    66061 tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga
    66121 cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg
    66181 actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat
    66241 cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa
    66301 actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat
    66361 cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc
    66421 ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat
    66481 cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga
    66541 tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt
    66601 ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat
    66661 catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa
    66721 cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg
    66781 cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt
    66841 tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga
    66901 acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga
    66961 cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt
    67021 tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga
    67081 caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt
    67141 ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag
    67201 cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg
    67261 ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg
    67321 caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa
    67381 acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag
    67441 ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat
    67501 tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa
    67561 acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa
    67621 aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc
    67681 gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag
    67741 cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat
    67801 taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata
    67861 agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa
    67921 tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa
    67981 gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta
    68041 ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag
    68101 aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt
    68161 tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg
    68221 aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag
    68281 aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac
    68341 aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg
    68401 aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg
    68461 caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag
    68521 atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat
    68581 ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt
    68641 tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa
    68701 ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg
    68761 gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat
    68821 cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc
    68881 ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg
    68941 ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg
    69001 ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg
    69061 ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca
    69121 gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg
    69181 atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta
    69241 acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg
    69301 cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg
    69361 cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta
    69421 gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa
    69481 atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt
    69541 acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa
    69601 ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac
    69661 ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc
    69721 ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt
    69781 gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt
    69841 cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata
    69901 gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat
    69961 taattgcctt gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac
    70021 taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg
    70081 tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta
    70141 tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc
    70201 acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc
    70261 tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg
    70321 ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca
    70381 ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg
    70441 tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg
    70501 aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg
    70561 tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg
    70621 gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct
    70681 ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa
    70741 gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc
    70801 aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg
    70861 gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag
    70921 ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa
    70981 aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg
    71041 aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca
    71101 cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat
    71161 ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga
    71221 agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat
    71281 caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga
    71341 agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga
    71401 cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc
    71461 tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg
    71521 ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg
    71581 tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt
    71641 gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc
    71701 tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa
    71761 aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat
    71821 cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg
    71881 ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga
    71941 agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac
    72001 cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg
    72061 ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt
    72121 ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg
    72181 agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat
    72241 aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca
    72301 gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg
    72361 acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta
    72421 gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca
    72481 gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt
    72541 gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat caattttact
    72601 ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg
    72661 agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt
    72721 ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg
    72781 gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa
    72841 gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta
    72901 gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc
    72961 aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa
    73021 taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc
    73081 gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta
    73141 caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt
    73201 tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc
    73261 gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca
    73321 tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa
    73381 tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga
    73441 gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt
    73501 ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga
    73561 aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt
    73621 gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc
    73681 tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa
    73741 tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt
    73801 tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg
    73861 ttgtgaatct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat
    73921 gtttgatgaa tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa
    73981 aaaagggact gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt
    74041 attttttagc aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa
    74101 tttcaagatc catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta
    74161 atgagttacc atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt
    74221 catcttgcca gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa
    74281 tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt
    74341 cttccattaa tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc
    74401 cgtatttttc agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt
    74461 ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt
    74521 tacatgtttt gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag
    74581 cttctttgat tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta
    74641 catcagccca accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa
    74701 aaactaattt tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc
    74761 agttctgttt tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt
    74821 ctatgagaaa acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt
    74881 ttaggtatta gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac
    74941 atgttccaac tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat
    75001 aatagagacg tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt
    75061 ttcctttttt acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact
    75121 ccatgtttgc taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga
    75181 ttccttcatc aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg
    75241 ttaattgccg cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt
    75301 gttgcatgtc agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt
    75361 catatgtctc atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg
    75421 ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt
    75481 tctagcagtg attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa
    75541 agatagtttt ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc
    75601 tttatttacg acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt
    75661 tagaaaccat gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg
    75721 tagctaagct ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac
    75781 catctgtggt taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt
    75841 cttcaaaaat atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga
    75901 acttcccaag tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt
    75961 acgtagcgac tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat
    76021 cagcactgat ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac
    76081 tctttaacgg tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc
    76141 ctgtgatacg taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg
    76201 cggttgaacc ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa
    76261 tgccagctaa ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg
    76321 ctttcctcac tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat
    76381 aaagcttttt caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct
    76441 aacaaaggtg ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca
    76501 tatttgataa ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga
    76561 tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca
    76621 attagtacgt aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa
    76681 aggatataac agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt
    76741 agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc
    76801 acgtgttcgt ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa
    76861 cttacaagaa cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt
    76921 acgttaccat atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag
    76981 ccgttctaaa tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa
    77041 cttgcttcta ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg
    77101 atgccacgta aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca
    77161 aaattagtaa ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct
    77221 aacattatct ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa
    77281 gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt
    77341 gggggttcaa actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt
    77401 cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta
    77461 gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac
    77521 actcataaaa tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc
    77581 tgtttggtcg gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata
    77641 gtaaagagag gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat
    77701 atcggtatca tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac
    77761 tatactggta aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg
    77821 atggaacaag aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg
    77881 aagggtcacc gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt
    77941 gaacgttcat tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt
    78001 gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta
    78061 tttgcaaaca aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat
    78121 gaccgtttgc aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc
    78181 acaggaatta tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca
    78241 gagatcgaag aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa
    78301 aaattggttg aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt
    78361 gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa
    78421 ttctacccag tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac
    78481 gctgttcttg attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct
    78541 aaaactgatg aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca
    78601 tttaaagtta tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt
    78661 gttttacaat caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt
    78721 cgtatcctac agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat
    78781 atcgctgctg ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag
    78841 agtctagtta ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa
    78901 cctaaatcaa aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa
    78961 gatcctactt tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg
    79021 ggtgaacttc acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct
    79081 aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa
    79141 ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt
    79201 acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt
    79261 cctcgtgaat acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc
    79321 ttagctggct atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat
    79381 gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct
    79441 aagaaagcta atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa
    79501 gattatttag gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg
    79561 gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt
    79621 ggttatgcta caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt
    79681 gaccactacg aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga
    79741 aaagctgaat aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat
    79801 atttttaata tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg
    79861 tgtacatcat atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt
    79921 aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga
    79981 cacgttgacc atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa
    80041 aacggcggcc aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc
    80101 ggaatcacaa tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac
    80161 gttgactgcc caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg
    80221 gacggagcta tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac
    80281 atcctattgt ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac
    80341 atggttgatg acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca
    80401 gaatacgaat tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta
    80461 gaaggcgacg cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac
    80521 atcccaactc cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc
    80581 tcaattactg gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt
    80641 ggtgacgaag ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt
    80701 gttgaaatgt tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta
    80761 ctacgtggtg ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca
    80821 atcacacctc atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga
    80881 cgtcatactc cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta
    80941 acaggtgttg ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca
    81001 atggaagttg aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt
    81061 gaaggcggac gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa
    81121 gttttaaagt aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc
    81181 tttgttccga gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat
    81241 agaagaaaca cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat
    81301 gttttttaca agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc
    81361 gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga
    81421 ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct
    81481 atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat
    81541 ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac
    81601 tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct
    81661 ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg
    81721 tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat
    81781 gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa
    81841 taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat
    81901 cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt
    81961 actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga
    82021 tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc
    82081 tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa
    82141 agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa
    82201 aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca
    82261 cggttctcgt taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt
    82321 taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga
    82381 agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc
    82441 taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag
    82501 gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga
    82561 aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc
    82621 aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc
    82681 tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg
    82741 atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc
    82801 ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga
    82861 aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac
    82921 aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct
    82981 agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt
    83041 aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca
    83101 aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa
    83161 cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa
    83221 gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt
    83281 aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat
    83341 gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa
    83401 atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga
    83461 aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt
    83521 ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa
    83581 accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca
    83641 aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc
    83701 aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg
    83761 gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag
    83821 gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta
    83881 ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg
    83941 gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg
    84001 gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac
    84061 aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa
    84121 ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag
    84181 ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa
    84241 ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat
    84301 tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga
    84361 aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa
    84421 agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat
    84481 ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg
    84541 taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg
    84601 ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca
    84661 tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt
    84721 gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac
    84781 aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac
    84841 tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca
    84901 atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt
    84961 catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca
    85021 tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa
    85081 agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact
    85141 tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc
    85201 aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct
    85261 aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa
    85321 aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg
    85381 tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca
    85441 agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg
    85501 atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga
    85561 agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat
    85621 tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt
    85681 gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa
    85741 gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa
    85801 atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt
    85861 aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt
    85921 aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc
    85981 gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca
    86041 atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga
    86101 aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt
    86161 gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga
    86221 agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg
    86281 gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg
    86341 cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca
    86401 ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa
    86461 cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa
    86521 acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt
    86581 cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag
    86641 tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg
    86701 tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc
    86761 aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa
    86821 atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat
    86881 tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact
    86941 acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga
    87001 cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt
    87061 tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga
    87121 taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc
    87181 tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt
    87241 tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg
    87301 tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta
    87361 ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa
    87421 atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg
    87481 tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt
    87541 gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt
    87601 acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt
    87661 cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa
    87721 aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc
    87781 agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac
    87841 agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa
    87901 ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc
    87961 tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt
    88021 ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat
    88081 tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga
    88141 tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg
    88201 gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc
    88261 ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg
    88321 atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt
    88381 atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac
    88441 ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt
    88501 gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag
    88561 gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac
    88621 agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac
    88681 tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa
    88741 cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt
    88801 tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc
    88861 acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac
    88921 accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc
    88981 gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc
    89041 ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac
    89101 tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc
    89161 tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac
    89221 gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa
    89281 aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg
    89341 gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa
    89401 ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt
    89461 caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag
    89521 ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg
    89581 tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat
    89641 accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat
    89701 taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt
    89761 tgtcggtgac aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga
    89821 agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg
    89881 ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc
    89941 tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc
    90001 tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg
    90061 cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg
    90121 caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt
    90181 actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta
    90241 ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg
    90301 atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact
    90361 agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc
    90421 acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg
    90481 acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg
    90541 gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa
    90601 agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac
    90661 tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt
    90721 agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc
    90781 tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta
    90841 aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg
    90901 tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa
    90961 aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa
    91021 aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag
    91081 cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt
    91141 cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct
    91201 gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac
    91261 gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg
    91321 ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca
    91381 ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat
    91441 attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc
    91501 ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa
    91561 tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca
    91621 gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta
    91681 caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt
    91741 aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc
    91801 ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga
    91861 agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg
    91921 atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt
    91981 gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc
    92041 gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa
    92101 tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac
    92161 acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa
    92221 aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga
    92281 tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat
    92341 ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc
    92401 tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc
    92461 aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat
    92521 ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg
    92581 tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg
    92641 cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct
    92701 ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct
    92761 aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat
    92821 tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc
    92881 aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt
    92941 tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca
    93001 agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg
    93061 tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg
    93121 ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa
    93181 cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat
    93241 tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat
    93301 gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa
    93361 tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca
    93421 ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt
    93481 ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt
    93541 cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt
    93601 gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct
    93661 taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat
    93721 gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt
    93781 gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag
    93841 aatatagaag caggtattgc acatatccat tctacattca ataacactat catcatgatc
    93901 actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga
    93961 agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca
    94021 atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa
    94081 gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact
    94141 ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc
    94201 attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa
    94261 gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta
    94321 tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct
    94381 acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg
    94441 tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt
    94501 gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga
    94561 tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat
    94621 tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt
    94681 aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc
    94741 aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt
    94801 tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac
    94861 agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca
    94921 tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa
    94981 agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt
    95041 tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa
    95101 atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa
    95161 agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag
    95221 gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat
    95281 gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg
    95341 tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt
    95401 gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga
    95461 aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg
    95521 ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga
    95581 tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata
    95641 gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct
    95701 gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc
    95761 ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa
    95821 taattgaaaa ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag
    95881 taatcatgga gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc
    95941 ttcgccccgc attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta
    96001 ttggacacaa tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc
    96061 cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga
    96121 tccgacagat ggttggaatg gtttttcaaa atccagacaa tcagtttgtt ggttcgacag
    96181 tagaagatga cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag
    96241 tgcgtgtgaa agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg
    96301 ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac
    96361 ctgatatcat tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag
    96421 tgatctctac gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc
    96481 atgatattga tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg
    96541 tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc
    96601 tagatctacc atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc
    96661 ccagtgaata tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca
    96721 agtagacttc acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa
    96781 tatgacgatc aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc
    96841 aaccttactt cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg
    96901 agaacgagat attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt
    96961 tggcatcgtc tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat
    97021 tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga
    97081 aacattagaa caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc
    97141 aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact
    97201 tgtattggat gaaccaacgg caggacttga tccccagggt agaaaagaaa tgatggagat
    97261 gttttggcgt cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga
    97321 tgatgtagcc aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag
    97381 cggggagccg caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt
    97441 acctactgcc accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt
    97501 gcctttgaca gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca
    97561 tgatgaataa attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg
    97621 atccccgagc taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata
    97681 attgggaaac gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag
    97741 tagatttgaa attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg
    97801 tagctttgca ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc
    97861 atatcactga atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca
    97921 tttttatgtc gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg
    97981 agtacttgct tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc
    98041 tgtcgattgc tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg
    98101 ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag
    98161 tcattccttt gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga
    98221 ctgctatgga agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc
    98281 attggcataa tagagacacg atggttatca ttgcttttgt attattaact gtgctactga
    98341 tctttttgag aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt
    98401 tgcaagaaag gatgcgagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg
    98461 tcacaggcct ctttctttta tctgaaaaga aagaacgaaa aaattttata aaaaaatcat
    98521 tattccagat aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc
    98581 attgcatacg acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt
    98641 caagaagaag tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt
    98701 ggttcgggaa ggacagatgc cggagtacat gcgattgggc aagtaatcca ttttgattat
    98761 ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat
    98821 attgctgtaa gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa
    98881 gaaaagacgt atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat
    98941 tatgcaagtt attttcctta ccctcttgat ttggaaaaaa ttcagcgggc gcttcctgat
    99001 ttattaggga ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa
    99061 gtacgtacga tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt
    99121 acatttaggg gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg
    99181 aaaattggca atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat
    99241 cgtaatgcag caggacctac agcacatcct gaaggacttt atttgtatga agtgaaatat
    99301 gaataagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat
    99361 tacttctcaa aattttgcaa aatttcggct tattttcgaa ggagctactt ctggaacacc
    99421 gtgaatcaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaaacaaaa
    99481 atagctcgtc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    99541 ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg
    99601 aaaaatagtt cctcatattt tgccgtttgt tgagctaata ccgaaggtca gctgattgaa
    99661 caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa gtaacaattt
    99721 caaaaaacag cttctcatct ttgaggaata tcgtgacttg tggacgaggc gttgataagt
    99781 gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac gttgttttga
    99841 ttgcccttaa tgaggacagt cggaacaggt ttgtcatttt ta
//
LOCUS       DACEJR010000002        86748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEJR010000002 DACEJR010000000
VERSION     DACEJR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.002"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(379..735)
                     /locus_tag="IW651_000107"
     CDS             complement(379..735)
                     /locus_tag="IW651_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0812874.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(749..1159)
                     /locus_tag="IW651_000108"
     CDS             complement(749..1159)
                     /locus_tag="IW651_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0812875.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1505..3691)
                     /locus_tag="IW651_000109"
     CDS             complement(1505..3691)
                     /locus_tag="IW651_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0812876.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3909..4592
                     /locus_tag="IW651_000110"
     CDS             3909..4592
                     /locus_tag="IW651_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0812877.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4673..5392)
                     /locus_tag="IW651_000111"
     CDS             complement(4673..5392)
                     /locus_tag="IW651_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0812878.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5704..5910)
                     /locus_tag="IW651_000112"
     CDS             complement(5704..5910)
                     /locus_tag="IW651_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812879.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6155..6757
                     /locus_tag="IW651_000113"
     CDS             6155..6757
                     /locus_tag="IW651_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0812880.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6898..8179
                     /locus_tag="IW651_000114"
                     /pseudo
     CDS             6898..8179
                     /locus_tag="IW651_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            8192..8920
                     /locus_tag="IW651_000115"
     CDS             8192..8920
                     /locus_tag="IW651_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0812881.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(8989..10512)
                     /locus_tag="IW651_000116"
     CDS             complement(8989..10512)
                     /locus_tag="IW651_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0812882.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10764..11945
                     /locus_tag="IW651_000117"
     CDS             10764..11945
                     /locus_tag="IW651_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0812883.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12005..13633)
                     /gene="treC"
                     /locus_tag="IW651_000118"
     CDS             complement(12005..13633)
                     /gene="treC"
                     /locus_tag="IW651_000118"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0812884.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14084..16795)
                     /locus_tag="IW651_000119"
     CDS             complement(14084..16795)
                     /locus_tag="IW651_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0812885.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(16805..17506)
                     /locus_tag="IW651_000120"
     CDS             complement(16805..17506)
                     /locus_tag="IW651_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0812886.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17692..18291
                     /locus_tag="IW651_000121"
     CDS             17692..18291
                     /locus_tag="IW651_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0812887.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18372..19385
                     /locus_tag="IW651_000122"
     CDS             18372..19385
                     /locus_tag="IW651_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0812888.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19493..20410
                     /gene="rbsK"
                     /locus_tag="IW651_000123"
     CDS             19493..20410
                     /gene="rbsK"
                     /locus_tag="IW651_000123"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0812889.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20438..20833
                     /gene="rbsD"
                     /locus_tag="IW651_000124"
     CDS             20438..20833
                     /gene="rbsD"
                     /locus_tag="IW651_000124"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0812890.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            20833..22206
                     /locus_tag="IW651_000125"
     CDS             20833..22206
                     /locus_tag="IW651_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0812891.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22563..24707
                     /locus_tag="IW651_000126"
     CDS             22563..24707
                     /locus_tag="IW651_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0812892.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24759..25800)
                     /locus_tag="IW651_000127"
                     /pseudo
     CDS             complement(24759..25800)
                     /locus_tag="IW651_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(26077..27879)
                     /locus_tag="IW651_000128"
     CDS             complement(26077..27879)
                     /locus_tag="IW651_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0812893.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(27893..28645)
                     /locus_tag="IW651_000129"
     CDS             complement(27893..28645)
                     /locus_tag="IW651_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0812894.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28654..29016)
                     /locus_tag="IW651_000130"
     CDS             complement(28654..29016)
                     /locus_tag="IW651_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0812895.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29192..30064)
                     /locus_tag="IW651_000131"
     CDS             complement(29192..30064)
                     /locus_tag="IW651_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0812896.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30115..30603)
                     /locus_tag="IW651_000132"
     CDS             complement(30115..30603)
                     /locus_tag="IW651_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0812897.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30741..31532)
                     /locus_tag="IW651_000133"
     CDS             complement(30741..31532)
                     /locus_tag="IW651_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0812898.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31587..32195)
                     /locus_tag="IW651_000134"
     CDS             complement(31587..32195)
                     /locus_tag="IW651_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0812899.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32188..32505)
                     /locus_tag="IW651_000135"
     CDS             complement(32188..32505)
                     /locus_tag="IW651_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0812900.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32661..33389)
                     /locus_tag="IW651_000136"
     CDS             complement(32661..33389)
                     /locus_tag="IW651_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0812901.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33548..34429
                     /locus_tag="IW651_000137"
     CDS             33548..34429
                     /locus_tag="IW651_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0812902.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34666..35709)
                     /locus_tag="IW651_000138"
     CDS             complement(34666..35709)
                     /locus_tag="IW651_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0812903.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35712..36380)
                     /locus_tag="IW651_000139"
     CDS             complement(35712..36380)
                     /locus_tag="IW651_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0812904.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36522..38858
                     /locus_tag="IW651_000140"
     CDS             36522..38858
                     /locus_tag="IW651_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0812905.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(38973..39284)
                     /locus_tag="IW651_000141"
     CDS             complement(38973..39284)
                     /locus_tag="IW651_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812906.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39465..40940
                     /locus_tag="IW651_000142"
     CDS             39465..40940
                     /locus_tag="IW651_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0812907.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            40987..41571
                     /locus_tag="IW651_000143"
     CDS             40987..41571
                     /locus_tag="IW651_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0812908.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41573..41992
                     /locus_tag="IW651_000144"
     CDS             41573..41992
                     /locus_tag="IW651_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0812909.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42273..42557)
                     /locus_tag="IW651_000145"
     CDS             complement(42273..42557)
                     /locus_tag="IW651_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0812910.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(42789..43499)
                     /locus_tag="IW651_000146"
     CDS             complement(42789..43499)
                     /locus_tag="IW651_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0812911.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43496..44968)
                     /locus_tag="IW651_000147"
     CDS             complement(43496..44968)
                     /locus_tag="IW651_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0812912.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45106..45579
                     /locus_tag="IW651_000148"
     CDS             45106..45579
                     /locus_tag="IW651_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0812913.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45732..46283)
                     /locus_tag="IW651_000149"
     CDS             complement(45732..46283)
                     /locus_tag="IW651_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0812914.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46388..46918
                     /locus_tag="IW651_000150"
     CDS             46388..46918
                     /locus_tag="IW651_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0812915.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47401..47844)
                     /locus_tag="IW651_000151"
     CDS             complement(47401..47844)
                     /locus_tag="IW651_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0812916.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(47860..49020)
                     /locus_tag="IW651_000152"
     CDS             complement(47860..49020)
                     /locus_tag="IW651_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0812917.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49527..51227
                     /locus_tag="IW651_000153"
     CDS             49527..51227
                     /locus_tag="IW651_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812918.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51329..52162)
                     /locus_tag="IW651_000154"
     CDS             complement(51329..52162)
                     /locus_tag="IW651_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0812919.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52466..54631)
                     /locus_tag="IW651_000155"
     CDS             complement(52466..54631)
                     /locus_tag="IW651_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0812920.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            54891..57185
                     /locus_tag="IW651_000156"
     CDS             54891..57185
                     /locus_tag="IW651_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0812921.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57178..57843
                     /gene="pgmB"
                     /locus_tag="IW651_000157"
     CDS             57178..57843
                     /gene="pgmB"
                     /locus_tag="IW651_000157"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0812922.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            57836..58843
                     /locus_tag="IW651_000158"
     CDS             57836..58843
                     /locus_tag="IW651_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0812923.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            58865..59884
                     /locus_tag="IW651_000159"
     CDS             58865..59884
                     /locus_tag="IW651_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0812924.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60165..61532)
                     /gene="nhaC"
                     /locus_tag="IW651_000160"
     CDS             complement(60165..61532)
                     /gene="nhaC"
                     /locus_tag="IW651_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0812925.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61641..63062)
                     /locus_tag="IW651_000161"
     CDS             complement(61641..63062)
                     /locus_tag="IW651_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0812926.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63305..65182)
                     /gene="tdc"
                     /locus_tag="IW651_000162"
     CDS             complement(63305..65182)
                     /gene="tdc"
                     /locus_tag="IW651_000162"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0812927.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65473..66729)
                     /locus_tag="IW651_000163"
     CDS             complement(65473..66729)
                     /locus_tag="IW651_000163"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0812928.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67309..68610
                     /locus_tag="IW651_000164"
     CDS             67309..68610
                     /locus_tag="IW651_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0812929.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68703..70091)
                     /gene="ltrA"
                     /locus_tag="IW651_000165"
     CDS             complement(68703..70091)
                     /gene="ltrA"
                     /locus_tag="IW651_000165"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0812930.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(70578..71177)
                     /locus_tag="IW651_000166"
     CDS             complement(70578..71177)
                     /locus_tag="IW651_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0812931.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            71431..72312
                     /locus_tag="IW651_000167"
     CDS             71431..72312
                     /locus_tag="IW651_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0812932.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            72325..73935
                     /locus_tag="IW651_000168"
                     /pseudo
     CDS             72325..73935
                     /locus_tag="IW651_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(74012..74671)
                     /locus_tag="IW651_000169"
     CDS             complement(74012..74671)
                     /locus_tag="IW651_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0812933.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(74954..75619)
                     /locus_tag="IW651_000170"
     CDS             complement(74954..75619)
                     /locus_tag="IW651_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812934.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(75766..76371)
                     /gene="dhaL"
                     /locus_tag="IW651_000171"
     CDS             complement(75766..76371)
                     /gene="dhaL"
                     /locus_tag="IW651_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0812935.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(76399..77382)
                     /gene="dhaK"
                     /locus_tag="IW651_000172"
     CDS             complement(76399..77382)
                     /gene="dhaK"
                     /locus_tag="IW651_000172"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0812936.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(77384..77779)
                     /gene="dhaM"
                     /locus_tag="IW651_000173"
     CDS             complement(77384..77779)
                     /gene="dhaM"
                     /locus_tag="IW651_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0812937.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            77933..78253
                     /locus_tag="IW651_000174"
     CDS             77933..78253
                     /locus_tag="IW651_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0812938.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(78365..79234)
                     /locus_tag="IW651_000175"
     CDS             complement(78365..79234)
                     /locus_tag="IW651_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0812939.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(79330..80634)
                     /locus_tag="IW651_000176"
     CDS             complement(79330..80634)
                     /locus_tag="IW651_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0812940.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(80631..81140)
                     /locus_tag="IW651_000177"
     CDS             complement(80631..81140)
                     /locus_tag="IW651_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812941.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(81315..83450)
                     /locus_tag="IW651_000178"
     CDS             complement(81315..83450)
                     /locus_tag="IW651_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0812942.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(83533..85188)
                     /locus_tag="IW651_000179"
     CDS             complement(83533..85188)
                     /locus_tag="IW651_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0812943.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(85190..85504)
                     /locus_tag="IW651_000180"
     CDS             complement(85190..85504)
                     /locus_tag="IW651_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0812944.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            85674..85934
                     /locus_tag="IW651_000181"
     CDS             85674..85934
                     /locus_tag="IW651_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0812945.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            85948..86262
                     /locus_tag="IW651_000182"
     CDS             85948..86262
                     /locus_tag="IW651_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0812946.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        25531 a        17670 c        15473 g        28074 t
ORIGIN      
        1 tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc ttcacaagtg
       61 gttctttttt aacaactcag ttatcttatc atgtcatttc taacatgtca acaactattt
      121 tatatgtttt ttaaaatact attttattgc tttgctactt aaaacaacag aaatgaatat
      181 atcatgtaaa tttacacatg tcaatatatt tttaactcta tatccccacc tagatttgaa
      241 ttttgaccac ataaattgag catgtctcta tagtagaaaa ataaagtcaa aaaaagaaca
      301 cataacacgc ttgatgctaa gtgttcgact gttacaactg tatgaaatgc tcagtgactt
      361 gattaaaaca gttaaagtct atttatttaa gataagctgt tccatcaact gctcattgta
      421 tccaataaat agaacttcat cgataataat caaaggacgt ctgaggagat tggtatgttg
      481 tgtgcataaa aaaatcagtt gtttttttcc tacacaggaa aggtcaatcg caatgttgtg
      541 aaaagcctca gaccaagtcg caaatagctg ttctgcttgc acttcttcaa aactgcataa
      601 ttcttctgct tcttctgtac ttagatcgcg ttcttctaag tcaacaaatt gataaggcaa
      661 ttgatggtgt tctaaccatt gtttcgcctt cagacttgtt acacagtgcg ctgatccata
      721 gactttcact tccacgaagg ctccctccct atgaatttaa gatgttattg ttttgtaaat
      781 agatcagatt tctggcaaca catcgaattt cacgagggac aaatgaccgg atctcatctt
      841 cattaaatcc gattagtatt cttttttcat caatcaaaat aggtcgtctc aataatgaag
      901 gatattcttt gaataagacg atcatttgct ttaaagttaa ttcgttaaaa tctatgccca
      961 gcttttcgta aattttcgac cgagtggaaa taatatcttg aactcctcct tcagtcgctg
     1021 ccaagatctt tttgatctca ctttctgtta acggttcggc aaaaatattc ttctcttcaa
     1081 attcaagttg attcgcttgc aaccatgctc ttgcttttct acaggaagta cagctaggcg
     1141 aagtatatac ttttatcatt ttctcactcc tttttaatta taatccaatt ctacagtaat
     1201 ttcttttaaa ataccaatta ttagctaata aaatataaaa aaaatcaaaa taactgatta
     1261 tttacgtttg aataatgaga atttttctat atattctcta ttttgataat agtattctca
     1321 ttttcaaaat aaataaggga ggtgtgacaa aattcttgtc acacctccct tttccgaata
     1381 aactgtgttc cagaaatagc ttcttcgaaa ataagctgaa atttcacaaa aatttgagaa
     1441 gcaattttcg tacattcctt cttctttctt gaaactgacc acttctgtca caccctctct
     1501 tctatcactt tccttttttc acacgactcc cggtttcttt gataaatctc gatactttca
     1561 attttcttga atttttcatt tgaggtacac atagatacag acgatttctt gctctagtca
     1621 tcgcaacata aaatagtctt cgttcttctt ctatcgtcgt tactggttct gcttttattt
     1681 cattaaagtc gattactttc ttttctgtcg ctccgttata aggcaaactt ccttcgttac
     1741 aaccaatcaa aaagacattt cggtaactaa gacctttact tttgtgaatg ctctggatac
     1801 atagtgcatc aagttttcct tgtctgtaag caatcaagcg ggtttttaat tctttagctg
     1861 tcgcttgggt ttcttgcgat aaggcttgga gtcttctgaa agtagcatgc tgctttactt
     1921 cttctcgtat ccattcttga tcactctggt cgatttcctt agctatacgt ggcagatgaa
     1981 gatagctttg aacaagcgaa tagatttctt gatgctggtc tgttttacgc aacgcttcct
     2041 caaactctcg gatttctgca gatttcgatt ttaaattggg gcttatcgac aaaatataat
     2101 ctgcaacgga tatattttga tatttaggag aaaagtattg atcgcattca ctttttttca
     2161 ggtagcagtc tgccatcagc tgccggagca attgctggta ggcctgattt ttcttttgca
     2221 gattcagtgc tgtttgccaa tgaacaattt tttgatagaa atgttggaaa gacttgatac
     2281 caaagatacc ttcatatgga ctatctgctt ccagtacaaa aggaatattc gttttcagca
     2341 gagattggac aagctgctgc gtttgtgaag ctgatcggta taatatgatt gattccttat
     2401 atttcccatt tttttgaata aatgtctgaa tttgtttgat tagccactga ctttctgctt
     2461 tagtatcaga aaacaaatag gcttccataa cacctgcttc tttttgtgct ggaatcatcg
     2521 cctttggtat ccgatcattt ttattatagc tgatcaatct atttgctcct tggacaatag
     2581 ctgcccgaga acgataatta gttaataaga aaagagtttt ggtaggatat tctttttcaa
     2641 agttcttgat gtatgctggc tctgatcctc tgaatccata gatactctga tcatcatctc
     2701 caacaacaat caacttgatc ctgttttcct ttgtcatccg tttcacgatt tcccattgaa
     2761 gtggattagt atcttgaaat tcatcaatga atagataatc atatttttgg ccgatataat
     2821 gctggatctc ctcattctcc aaagcgcttt tcagattttg gagtacatca ttgaactcta
     2881 gcaattgctg gcttcttttt tgttcttgga gttccgtgag caatccatac agtcgttctg
     2941 atatcggctg tcgtgcattc ttatcaatgg aatcatattt ttctttaact aactgaacca
     3001 tttcttctac tgaaaggttc atattctgcc aatagtctaa ccgagaagca atcgtttttt
     3061 ctccatattg atcaaatata taaagattat atttacgtat cagctgttgc aaaacagcat
     3121 tcactttttg tgcagtatcc tttgaaaagt ctccttgtat tccttgcatt gctacttcag
     3181 gaaattttcg acaaatttca ttgtatatcc taaaaaagat actatggaat gttccgatat
     3241 ggacagcggt ttttctctgg ctgaaattat ctactcgttt ttttagttca tttgcagcgt
     3301 tccttgtaaa agtgatcatt agtatgcgtt cttcgtctac ttgtttttgc gtcatcagat
     3361 aaacagcttt tgcacagatt gtttgcgttt tgccgctccc agctcctgct atgattcgaa
     3421 gatgtttctt catatcatat gtgatagctg ctttttgttc ttgattgaag cgattatagg
     3481 aacgtttctc catcatttta aagaaagagc tgtttccttc actttctttt acttgtactt
     3541 cttcctcaat aagtccgggt gcttcgccat aagccgcttc ataggtagca atgatttctt
     3601 cttgtagccg aaagacttgt tgaagctttt tatacttgtt ctcccagcgt ttcaattctt
     3661 tttgtaatcg gagaatttct ttttcattca actgattgat ttccttccca tctttcttct
     3721 ttgaatttgt ttatttcaag ttggttattt aaagtttcgt tgtctatttt ttctttatac
     3781 taacagacct ttctaaaaat gaaaatggga agcgatatga ttttagtttt ctgaaaattt
     3841 ttcctttttt tcaatcgttt atgctaagat aaggaagaca aaaatattca taaaaggtga
     3901 tgaaaaaaat gcagacttcc tttcacgagg ggcgtacaat aggacttttg ctgacattta
     3961 tcggaggagc catggacagt tatacttata tccaatatgg tgcttttgcc tctgctcaaa
     4021 ctggcaatat cattctatcg atcatccaag catttgacgg agaatggcta agtgttggta
     4081 aaaaagtatt gtctaccctt ttctttttct tagggattct tctaacaaag tatctaattg
     4141 attattttag aaaaaaagaa aaacatttct ggcgcttgtt tgttctatat tatgaagcag
     4201 tgattttttt ctttgttagt ttgacacccg tccatgagca tcccgcatta gtaacgattt
     4261 tgattgcttt tactgcatcg atccagtggg tagcttttga taaaatcaac gggcgtgcct
     4321 acactaattt atttacaact ggaaatttaa aaggggtagc aacgaactta tatgattatt
     4381 gtatttctaa agaaaaagca gattttgatc gatttttcca ctatctaagt gtcgttcttg
     4441 cctttatcgc aggagctatt gtccttgttt tctgtcatca tctgtttggc gcccattcga
     4501 ttttgatcgt ttccatttta tttttgatca tggccctcac tcagacattt catttatggc
     4561 aattttatca tacgaatcgg ttcactattt gatctcaatg aatcaaaaaa actaagcaag
     4621 atgctgctca cagagaaata tctgcgagtg agcaatttgc ttagttttta tattatttag
     4681 ttgtaaagat tccctttgct ttcgggttat ttgtagtgta gcggatcgac tgggcatctg
     4741 cctcttctgt cattttcttt gcccgcatct tgatttcttg tacgatttgt tctccacatg
     4801 ccggatcggc tacgaaatca tatttgtccc catcgatcac tattttggga catatatcaa
     4861 actgttcata ccactcttcg tatcgtcgat tgagttcttt atagtaggaa tagagttcag
     4921 gatcttgttc tagctgttca tattcccggc ctcttttttt gattctcgct aacatagttt
     4981 caaaggatac acgaatataa accagtagat ctggtttctt ttgtggcgaa atttcttcta
     5041 actccttcag catcgtttct aaaagacctt catactgctt tacttctact tcagaaacac
     5101 gccctaaatc tgcattcaaa tgaaacagca tactatcttc gtagatggag cggtctagta
     5161 cgttgtcatc atggctgaaa gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa
     5221 tctgtaataa aaaagtatac ttttctggat tagaataaaa tagcggcaat acttcattat
     5281 cttccacgtt ctcataaaaa ggttttgttt ctaaatcttg ggcaagcatt tcagttagtg
     5341 tgcttttccc tgcaccgatc gttcctgcaa caatgatcac aattatcggc atccttcctg
     5401 taataaaatc tgttcttcat tttactactt catttataga aataaaagcc ttgactttat
     5461 ctgctcctta aaagctactt ctctaaaagt agccaaaagt aactcctatt catgaaaata
     5521 aaggctgtga caaaagtctt gtcgcagccc ttttttcaaa taaacagtat tctcctgcca
     5581 acacctcggt cacaaggcaa tattcataaa acatgaaaag ctgtttttta acattattcc
     5641 ttgtaactca gagctaaacg ggagactcac aacctcttta tgctctcaac attttatctg
     5701 tttttattct tctattgtac ttgatttgcg tacaactaac tgccagtgca gattatgagc
     5761 ggtatcattg tcttctactg cgcatttcat gacacgtatc atcacactat tttcatagat
     5821 tttttctatt tttcctttta ttggtgattc aaataaatca gatttaaatg catatgtttc
     5881 gccgacttca aacaattgtt cattttccat aagaatccct ccatcacctt ttataaggtc
     5941 agtaattttt taacataaat cactcgaaaa agcacaaact tgtcataaaa agtacttttc
     6001 gtatcaaaac tgtcactttt tttggtaagg ttcttggaaa acgaagattt ttttaggaga
     6061 tcatgacggt tttgatgtca aaaccacttt ttatgaaaca aaagacgttt tttatttttt
     6121 ttagcataaa atttgtggtg gaggtacgta tgagatgaaa gataaagttg tgaacaaatt
     6181 attagaagcc ttattcagcg gagacaaatc ggaagaagat atggtgtatg tccaagtaaa
     6241 atttgtctta gaagttttat tgaataatct tggaaaacta gcggtcgttc tagtcttttc
     6301 tctggtgaca ggctcatggg cggaaacagg aatcaccttt ctttcttata tctgcatccg
     6361 cagatatgct tatggattgc attccgactc tgaattcgtt tgtctgctgt ggacactact
     6421 ttatttatgg ggcgttccct tggtgatgaa acatctgcaa ttgactatct ccttcccaat
     6481 gatggtcttt ttgttaatca gctgcttttt gttactgctt cgctatggat caagaggtac
     6541 agcaatcaat cccattgaac ccgagaaacg tcccccatta ttaaagaagg cgatcagcat
     6601 gtttctaatt ttttctctca ttacgttatt tttctcagct tcttactttt ctacctattt
     6661 gctacttggg attgttttag aaatagctac gttgcttcct attacaaact acttgatgaa
     6721 ttttggagga atctttcatg aaaaaaacaa tcattaaaca attaactaac ttatctgact
     6781 tttttgtatc tggctgctgc tttggtatga gttctgaatt caaaatccct gaaaaattaa
     6841 aaaaataata cgccaattgt gctataattt ggtaaaaatc aatgggagct gtactttatg
     6901 tttttatggt ccatagcgtt gcaatatttg atttacacaa tttgtatctt attgattgac
     6961 catcacatcc gatacaaata tctgtttgcg ggagtcattc tgatttcttg tattttgatt
     7021 ggatgggtct attcgatttt agggattttt actgctctaa ttgctatagg aatgctgatg
     7081 cttacttttt tcttcttttc gacttcgcgt caacaattgg tttacgcgct ttctttttgg
     7141 gttattgacg tcgcttttag gagatcactt aacaagcatc ggcgattttt ttgttttgaa
     7201 atcttcggtt attgaaccag gagattcatt gcagtatttt cacattgtct tatccgcttt
     7261 tttatctgct ttattagctt tttgtttaaa aaaatcgtta gagacttggt ttcacactgt
     7321 ggaccgccgt atgatcggta cgatcgggac attgatatta tttacatact atatcattat
     7381 tttttatacc cgtttttctg gtgaaacacc tgatgtctta gctttgaata cgagtttttt
     7441 cattctatat ctgtgtgttg gcctgatcat cttggtttat tattggaaaa tctctaataa
     7501 gaaattagct gatcagttat tagagcaaca agtcaggatg caacaggatt atatcaaaga
     7561 tctagaaaaa aactatcaag acttgagaga attcaaacat gattaccaaa atctgctttt
     7621 ttctcttcac agctatattt cagaaggcga cttagaagga ttacggtctt attatgatca
     7681 aacgatatta cctacacgcc agatgatgaa cctttttccg gccaatctaa gtatgttgga
     7741 taatatgaaa gttcctgaga tccgtagttt actgtcatta aaactcatga tggcacagga
     7801 aaaagggttg atcgtccagc ttcattttcc tgagccaatc gaactggata cgaaacatac
     7861 ggtaaacctt gtacgtatgc ttggtattat cttggacaat gcgattgaag gagctagtgc
     7921 tgcaaagaag aaaaaaatcg aattgaccat actcaagaag aatcattccg tgatgattcg
     7981 tgtgatcaat acaacaacaa atacattacc gttaaaccag ttgaaacaaa aagggttctc
     8041 aacaaaaaca aatccaaaaa acgaaggcct tggcttgttt atcttggatg aattgacaaa
     8101 gaccaatcct tatcttttcc ttgaaacatc aattgaaaac cagcgttttt ctcaaacgat
     8161 ttatatacaa ataaaataaa ggagtaatga tatgattccc atttatattt gtgaagacga
     8221 tccaaaacaa ttagaagttt tatcaacggt gatcaaaaat agaatcatga ttgaaaactt
     8281 agacagttat gtccatattt ctacctctga tccggaagaa cttctcaatg cagccaagat
     8341 cagaacttct tcttttggta tttacttttt agatatcgaa ctagaagaca gtgaaatgga
     8401 tggtgtagcg atcggtaaat tgatccgtga attagatcct ttaggaaaaa taatctatgt
     8461 gacttcccat acggaaatgt cgatgaaaat cttaaaaagc aacatcgaac cgactgatta
     8521 tatcgtcaaa gaagatcttt atgatttaaa agaaaatgtc gagcatatcc tgagcaaaat
     8581 tttcgaagat tttcaaatgt cccaaatcca aaaagatatc ttcaaaatag agtttaatga
     8641 tgagatcaaa tttttaccga tcaaagacat ccaatatttt tctacttcgc taggagcgcc
     8701 acataaactg gaagtccatt tgactcatgc ccaattacaa ttctatgaga agatcaaaga
     8761 aatcgagaaa atgcatcgga attttatccg ttgtcataaa tcctatgtta tcaatacaca
     8821 aaatgtgcgg tcgattaata aaaaaaccag agaagtcacg ttggcaaacg gcgagaccat
     8881 cccagcctct atccgtggat taaagaaatt gatttcataa aaagaaccga atagtttgga
     8941 ccgactaaat gaccagccga tccaaaccct tcggttcttt tttgcctatc agtgcaaaag
     9001 catttcctgt gcaatttcac gtccatccat tgtatctgga taaaatgttg gccaattctc
     9061 catttctttc agcaatactt cttgactttc atttcctgca tataaatgaa aatgttcgga
     9121 gtcagccggt gcaatcgtgt gatcactgaa ttgaatatat ctaaaagcgc cactttcctt
     9181 atcttctgct tcgaatagat accttacgcc acggtttcct gcctcgtagg tcaagatttc
     9241 tttccctaca taacgataat ttgaacgttt cgttgttcca ttcttaaacg taaatgccat
     9301 ctctttctca ctgatcttga ttgaagcaat atctgtttga tagcccttcg tataatattc
     9361 tttatattct tgtgctgtct tatcctgatt caatttttct ttatattcaa aaacaggatc
     9421 aaaagtccca tcttctacat acgtatctac tgtttgccaa gttcctgccc aatctgaaag
     9481 agagcgatct tgtacatcct cgtcttcaaa ataaccgttg tagacgctct tttctttttt
     9541 gggggtcaaa gtatcttcta gcgggttttc tgtctctgtc gtttttttca acgcttcgag
     9601 gtttgcttcc atgatagaaa gatagttttc tccattttct atttgttcat tcgtcaatcc
     9661 ttccaaagga ttcaatactt ctaaagatac acctgcttcg tttgctaacg ttctggcaat
     9721 actatctttg gcgttttctt caaaataaat atagttgatt ccatgttctt taacaaagtg
     9781 tttcagttca gcaattctcg ccgcagacgg ttcttcacta gaagaaagac cagcaatcga
     9841 tacttgattc aatccatatt ctaacgctag atagttgaat gcagcatgtt gcgtgacaaa
     9901 agatctttgt tttgcttgct taaaggtttc ttgataaagc tgatggagtg cttgaagttt
     9961 ctcggtatag gctgctgcat tttttgtcca tacttcctgt ttttctgggt aagcttcaat
    10021 cagctgatca cgtatgtttg ctacctgctt gattgctaaa cttggagcta gccatacatg
    10081 tgggtctagc tcatgtgtat ggccttcttc cccgtgttca tggtcatgat catgtccttc
    10141 ttcacttccc ggaagtaaaa gcataccttc tgtcgctttt atcgtatgga cattgccttc
    10201 ttgtaatgtt ttttggattg cggggaccca tgtttccatg ttttcatcat tataaacaaa
    10261 gatatctgta tcttggatct ttgccatgtc tttagcagaa ggttcataat cgtgggaatc
    10321 agtccctgct ggaatcaata actctacttc tccttcatcc ccgacgattg cttttgtaaa
    10381 atcatacatg ggataaaagg tcgtcatcac gctcaatttt tctttactgt cctcggcttt
    10441 cccacttgcc ccgcaagcag ttaacatacc tgctaaaacc agcatggcca atatacctat
    10501 caatcgtttc atttatgaca ctcctcttct catcttcttt catttgaatg ttccgattcg
    10561 cttttcgcaa accgtaatag ttacgtttta gcaacaggat ttaatttatc atatggttaa
    10621 aagaactgtc aagcgttttc ttattttaat tgatgagaaa gtttatatga atgattgacg
    10681 atatttttgc aatcaacctc tatcattttc cttttatacc cgcttttgtc tataatgaag
    10741 ttatagataa aggaggaaaa agagtgaaaa aagaaacaga tacaacgaat cgtttgatcc
    10801 gctttttagg aggaaaaacc tcttactatg ttttaggaat agttatctta cttgcgctga
    10861 tcatttattt tttccatcag atctcgttta tattcaagcc tgtcggaatc attatcgcta
    10921 ctatcttgcc cccattattg tttgcactga tcttgtatta tcttttggaa ccagtcatca
    10981 aacgtttgag caagcacctt tcgaggaact tgtcagtcat tatcgtttat gtgattattt
    11041 tatttttatt ggggcttgga agcatatggc ttattccttt tcttgaagaa caggcaaaga
    11101 cattgatcag tagtttgcca gatttgtttc aagattttca agagagtctc cgacaatttt
    11161 tggagaaaac accttttgcc gattcattta atcaattttt cgcttctatt gacgacgtga
    11221 cgtctgatat agctggattt attggaaatt attgggaaag tggcgcacaa cggatcagca
    11281 acattttttc aactgttaca gctattttta ttactttatt tactggtcca attgtcgctt
    11341 ttttcctttt gagagatcct caaaaatttt atcagtcagt tttagcgatt gtcccaccag
    11401 catttcgtac agattttaaa aatttgacaa agattgctaa tcaacaattg ggttcatttc
    11461 taaaggggca aatcattgct tcatttatcc ttggagcagt ctactgggtt tgtttcttac
    11521 tgatcggatt agaatttgct agtgttttag ctatttctgc tgggttgtta tgtatcattc
    11581 catatattgg gccgtttatc gccttctttc caggtttgat cattgcattc caagactcta
    11641 cttttatggc tgtcaaattt gtcattgtct ggtttgccgt acaacttctc cacggtgact
    11701 tggtgattcc tcgagtaatg ggtgatcgtt tacagatcca tccgattacg atcttaatcg
    11761 ttttacttgt catgggtgat ctaatgggaa ttgtgggcgt tatttttggt attcctatct
    11821 atacgttagt caaattactc gttactttta ttttccgtaa attcaaacaa cgctacaata
    11881 aattttatgg cgataaagga gagtatgaaa actcttcatt ttctgaagaa gattatttta
    11941 aataaaacac aaaacgtcga tcttacgaga atagctcgta ggaacgacgt tttgtgtttt
    12001 tgttttaatt tttcagcaaa gcaattgcct ggtatggttt tagctccaga ctttgatgaa
    12061 gaacagtatc ggaataatta tcgataagaa tattggcttc tgtgaatcct tctgggatgg
    12121 cgatccttgt atctttatca tagaaatttg ttaatacaag cagctgctgt ccttcaaatt
    12181 ggcgaagaaa gccataaact tccggatgat ttgcttcata agcatgataa tctccttgtg
    12241 caacgattgg atatgttttt ctcagccgaa tcaatttctt gtaataggac aaaatagaat
    12301 cttcagaaga tagttctttt tctacattaa tctcttctag cttgcctcca acttttaacc
    12361 aaggtttccc tgtagtaaat cctgcttgtt ctgtgtctga ccattgcatg ggtgttcgag
    12421 agttatcgcg agattttgct ttgatccgac gaaacgcttc tttttctgtg aatccttgtt
    12481 caaggagtaa cttataagca ttcaaacttt ctacgtcaat atagtcggaa atcgatgaat
    12541 agtctgggtc gatcatgccg atttcttctc ccatataaat atagggcgtc ccacggttca
    12601 aatggatcat ggttgctagc atctctgctc cttgttttcg atactgatcg atagctacaa
    12661 atcgattgag cgcacgtggc tgatcatgat tattaaaaaa tagagcattc cagccgttac
    12721 cctcactcat cttttctccc catgtatgaa atagtcgctt taattctgca aaatcaaatg
    12781 gaggagtaga ccatttattc ccattttcat aatcgacttt caaatgatgg aaattaaaga
    12841 ccattgataa ttcttctcta ctaggttctg tatacaagat acaattttcg atcgtcgtag
    12901 agctcatttc accgactgtg atgatagtgg aatccgtccc aaacgttgct tggttcaatt
    12961 cttttagata cgtatgggtg attggtttgt ccgtatattc aggtttgcca tcattttctt
    13021 tattattttt caattgttcg tctttaccga taacattgat aacatcgaaa cggaatcctt
    13081 ttaccccttt ttccatccaa aaacgaacaa ctttgaatag ttcctctctt actgcttggt
    13141 tccgccagtt caagtcagct tgtgtccgat catataagtg caaataatat ttccccgtat
    13201 ctccaaatgg cgaccaagca cttccaccaa attttgatac ccaatccgtt ggctcttctc
    13261 ttaaaatgaa gaagtcctga taatgctggt cccctgctag tgcttttttg aaccattcat
    13321 gttctgtcga aacgtgattc agcaccatat cgagcatgat ttctatccgg tatttctttg
    13381 cttcggctat tagctgttcg aattcctcca tcgtaccaaa gataggatcg atggctgtgt
    13441 aatcagagat atcataaccg ttgtcttttt gagggcttgg ataaataggg tttagccaaa
    13501 tcatgtcgat gcctagatca ttcagatacg ggagtttttc tttgattcct tttaaatctc
    13561 caattccatc accattactg tctttgtaag atttgggata gatttggtaa atgactttgt
    13621 ctttaaatgt catggatatt tacctccatt caactgattt attacctacc acaacttata
    13681 tatacaagtt atcatttatt attataactg aaccgaaagc gtttgtaaaa tttatcttta
    13741 tacattctaa gaggtctgtt gcccaataaa aaacaagaga tcagacatgt atttgtctat
    13801 cctcttgttt aaaatagcaa aatattcttg tttcttctat aaatggtgtt caaaagcttt
    13861 tatctacggc aatcagtctt ctcaaatgta aaacatgagg agctaatttt atttgagtat
    13921 tctttttgct cgatgcagga cggctttttt acaacctctg attcatggtg ttcaatcagc
    13981 tgtccttcgg tattagctca aaaaacggca aaatatgaaa aactattttg ccgtttgttt
    14041 ctctaatact caggtcaaac cgctgatttc acaacctctg tttttattca ttcgatttca
    14101 acgcatcaat catatcgata tgctttaatt tgaaatgggt aacaatcatc acaataaccg
    14161 taaatacgat agttaatcct gctgataaga cataagccca ccatgtgatc actaatggaa
    14221 agatgacatt ctccatagac gcttgttgca agatgaagtc agtcaatata tatcctacac
    14281 caaatcctcc gatgattcct aaaagagtaa agatgatatt ttctcgaaca atgtacatcg
    14341 tgacttcttt atcaaaaaat ccaaggactt ttattgtaga taattcgcgt acacgctcgg
    14401 aaatattgat attagttaga ttatacaaga cgataaaagc cagcagtcct gataatacga
    14461 caaagattaa gacaaccgag tctaagttcg ccattgattc ttcttgcgtt tcgatttgtt
    14521 tggacatgaa agacgtattt gtcacagcgt ctgtggcaag cagtttctcg gaaagcattt
    14581 cttcttcttt tgttgtcatc ttatctgtac gcagcaaaaa tgcattatcc atcatcgatt
    14641 gatcagtaac tgaagcataa tattcagggg acatataaat aaagctacct agatagtttt
    14701 tcacgatttc ttttacttcg actttccatt ctttttgatc ttcatcataa agcgaaatat
    14761 gatctcctgt agataagtca aatagttctg ctactttttg cgtaatagcg attcctgttt
    14821 taggcaagga gcgtgggttc tcttcaagat cagttaaatg gataaaacga tccaaatcag
    14881 ctgttttact tggtacgatc actgtcagac tttgcgaagc atgtcctttt tttctcaatt
    14941 ctacggtttg agtatgaata gctaggatct catttacttt ttcttgcttc tgcagcatat
    15001 ccgctgcttt ttgacgacta tcttccgtgg atgaatccaa tgtaacgatt gcttgatagt
    15061 caatgatcgt tccaaattgt ttggctgaaa cagaacttag agaatctttc aatcccacgc
    15121 ctgcaaccat cagcccggca catccagcaa ttccgataat cgccataagc attcttgact
    15181 tatatcggaa gagatttcgg taactgattt tttgattaaa gctaaggcgt gaccacagcg
    15241 gagtgatgta ttcaagaaaa atgcgtttcc ctggcttcgg tgctttcggt tgaagcaatg
    15301 cagcaggccg ttctcttaaa tctttgaata aaacgatcat tgcagcgcca aatgcggcaa
    15361 tgaagaatgc ggcagctgct tgtaaaatcg gcggccagtc ataaaaaata agcgcactcc
    15421 caatatcata ccgctcgtta gacagctcaa aaatgatcct tggcaaaaga ttcgttccta
    15481 atatcgttcc taaaacaata cctatccctg aggctagact tgcatagatt gcatattttt
    15541 ttgcgatttc aactttcgta taacctaacg cctttaatgt cccgatttcc ctgcggtttt
    15601 cttctaccat acgtgtcatt gtcgtaaagg tgatcaatgc ggcaataaag aaaaagaaaa
    15661 caggaaaaac attggcaatc gcagcgatac gatcagacag atcaccaaat tcttgaaaac
    15721 cgacattttc tgaacgttca ttgtataggt atgatggttt agttaaattt tcaatttttt
    15781 ctgcatttcg attcaattct tcctctgcag aggccagttc acgttctttc tcaactaatt
    15841 gcctttcttg ttcagcaagt tgagcttctt gttgagataa tgtttgaaca gcgattgttc
    15901 gttgcgcttc aggaagctgc tggatagttg ctgactgttg cgctaactgt tttttagcat
    15961 cttctaaccg tgttcttgct tgcgttgttt gtgctttgcc atccgctatt ttttgctgtg
    16021 ccggttctaa ttcttcgttg gccgattttt tcagctcttc taagcgttct tcgggtcgat
    16081 cttgtaaata cttttctgcc ttttcttgat tttgctccat tttatctttg taagctttgc
    16141 tgtacgtatt caaattcttt acgtttgtaa aatcaagata taagacagaa taaatatctg
    16201 ttttaaaaac ggattcaggt aaatagacaa aataatcgac gctaccattt ccgacgttcg
    16261 cgtatcctct ctccaaattg ttgatgaaca atggagaacg gacaaagccc gtgatggtat
    16321 actctttttc tttcaattca tctgaatcaa tctgataaat atcaccgatt ttataccctt
    16381 catcttccgc tttttcatcc aatacaagct ggtttggttt ttccgggagt tttccatcag
    16441 ttaattgtag cttgttctgc ttgttatttt tgttataaga aaatatttga atcacttcgt
    16501 ttttgccagt attggaataa tagatctgtt ttcctgtttc tacttttgca tctgggatct
    16561 tttctgcttg agcaatatct ttttgtgtca agccaccagt agaaacgatt tgcaaatcag
    16621 aaagtcctgc tgtaccaaca tagtgatcca tcgttttttg catgacaggt ccagcagttt
    16681 tgacacctac aaataaaagt gttcctagca tgacaataag gatgattgca atgaatcgtc
    16741 ctttagattg ccgaatttcg cgaaaacctg ctttaagata tgttcttgtt ttcataaaat
    16801 ccccctacca tacaatctca tcaatggatg aaggatgatc atttaattca acagacctta
    16861 ctttagcatc attaatgtga atgactctat cagcgatggg cgctaaagca gaattatgtg
    16921 taatgatcaa aacggtattg ccgtgttgtc tgctagcgtt ttgcaataat ttcaatactt
    16981 gcttgcccgt ttcaaaatcc agcgcgcctg tcggttcatc acataaaaga agtttgggat
    17041 ttttagctag tgcacgagca attgaaaccc tctgctgttc gcctcctgat aattgggacg
    17101 gaaagttatt caatcgatga gctaggccca cttgcctcaa tacctctaca ggatcaagcg
    17161 catcaggaga aacttccgtt gccaattcca cattttcttt tgctgttaga ttaggaacga
    17221 gattgtagaa ttggaaaacg aaccctacat ctgttcgtcg gtaagcagta agctgtttat
    17281 ctgaaaattg cgcaatatct gtgtcatcaa taatgatctg accttcatca ggtgtatcca
    17341 tcccacctaa aatattaaga atcgttgact ttcctgctcc acttggacca agtataatca
    17401 ctaattcgcc tttttcaatt gaaaaagaaa tatcgtcatt tgctgtaatg gtcgtttctc
    17461 ccattttgta tcgtttgtat tcatttttta cttcgacata cttcactctg attcctccat
    17521 tttcacgata tttctcgcta tatctatgca tcgaatgcgt tttctcacac ttatttattt
    17581 taccactgaa tttctctaga ctaaagaaag gaatccttta atttagttga caaataaacg
    17641 atttatgatt tacttacata aaaaacattg ttatttgaaa gaaggaacat catgaatgat
    17701 ttaaaggaaa atatgatttt tgatttatca aaaactgctt ttgtcgcgat cgacatgcaa
    17761 aaagagatcg taaataacgg tacactctct ccacacactg cagcatctat tctgacaaac
    17821 aatgatttgt tagtaagaac attgaaacgt accgctgcct tgaaagtttt agtgaacgta
    17881 gacatctcca ctttccctta tttgagtcaa caaactgata tgggtggaat gactgcttct
    17941 gtacctccag aattcactga tttacttttg aaggattctt tgaaagatac agataatatg
    18001 ttgactatca caaaatacaa tccaagtgct ttttttggta cttctcttga tcttcaactt
    18061 cgccggcgtg gaatcgaaac tatcatttta agcggtgtag cgacaacaaa cggtgtatat
    18121 gctactgcat tagatgcctt tcaacacggg taccatatcg tgcttgctga agatgcttgc
    18181 agtgatcgtg acaaagaatc ccatcaattg ttcataaaaa agatatttcc gaaaactgca
    18241 cgagtacgct caacgaaaca aataatcgaa gctatccaac aaagcaaata agtactggca
    18301 catgtgattt tcatttactt ctttttgatt tcatgggaaa ataaccacag gatttagata
    18361 ggagtatgaa catggtgaaa aaaaagatca caatcaaaga agtcgctaaa ctttccggcg
    18421 tttcaatcac aactgtctcc ttgatattaa atggaaatac ggagaaattc agtccgaaaa
    18481 ctgttcagaa agttttagct gccaaagaag aattaggcta tcagcctgac tattttgcta
    18541 gacaaatgat taccaaggaa acgaaaacaa ttggtgtact cgttcctgat atcacgaatc
    18601 cattcttcag tacactgatg cgaggaattg aggatatctt gtataagcag aatttcgtca
    18661 cgatattatg taatgctgat tctgatcatc aaaaagaaat cgaatatcta gccgagctca
    18721 cacgtcgagg tgtggatgga ttcattatcg cgacttctgc tgtctctaca gatgcaatca
    18781 acgaaaattt gaaaaaacaa ggccggccct tcattgtttt ggatcagaaa aaatctgaag
    18841 gatttagtga tgccgtacga acagacgatt ttcgcggtgg ttacctagcg ggcatgcact
    18901 tgcttagtct aggacatcaa acgatcgccc ttgtctatcc tgaaaatcca ccggaaaatg
    18961 tccacgctcg gattgagggg tttaaatctg ctcttgatgt ttaccaaatc cctcacgacc
    19021 aactgatcct cttacctaca cagttctcaa aacaaggtgg ctaccagatc acagcagaac
    19081 ttcttgattc tgctgcaact ggtgtgtttg ctttaaatga tgaattagct tttggattat
    19141 atcgcggatt agaggaagct ggaaaaagca tccctgaaga ctacagcatt attggctatg
    19201 ataatataga tatgtgcgag tatatcaagc cgaaactgac aacgatcgca caaccaatct
    19261 tcgaattggg acaaacatct gccaagttat tgttagatcg gatccaattc ccagaaaaag
    19321 aatgggaaga aaaaagatta cctgtacgat tagaaaaaag attttcaact gcacccttga
    19381 aatagctttc aaggtgtgct atatttttat cgaactatta aaacgtttta ctaaaacgtt
    19441 ttaaaaaata attttcccat caaaataaat gagatagttg gaggtttgta aaatgaacac
    19501 ggttacagta attggaagta ttaatatgga cactaccctt cgtttaacca acatgcccaa
    19561 gccaggcgaa acgatgcatg cgcatgagat ttttcacgct ggcggaggta aaggtgccaa
    19621 ccaagcagtg gcagccaaac gttcaggcgc acggacatct tttattggag gcgttggggc
    19681 tgacagcgaa ggacaacagt tattagatct actgacaaaa gaaaacatcg atacatctgg
    19741 gatagctgaa atccaaggcg ccacaaccgg acaagcaatg attatggtag acgcagcagg
    19801 agaaaatagt atcttgatcc atgcaggggc aaataatgct ttccatgaac aagaagtcct
    19861 aaaaaacaag caactgatta caaacagtga ctttattatt gctcagtttg aaagtagctt
    19921 agatgcgacg atcctagctt tttctattgc aaaagacgca ggaaaaacaa cgatcctgaa
    19981 tcctgcacca gctcgtgaaa cgattcctac tgaactactt gaaaagacag atatcattat
    20041 accaaatgaa acagaaacag aaatcattac aggaatcaga gtgacagatc ataacagttt
    20101 ggtcgcagca gccgaaaaac tccatgaatt aggtattgga acagtaatca ttacactagg
    20161 cagtgccggc gctttctatc atacagaaaa agaacacggt atcgtccctg cttttaaagt
    20221 ggatgccgtt gacacaacag ccgcaggtga tacatttatt ggcgcattaa gcagcacact
    20281 tcagccagac cttagtaact tgaaagaagc gatcctttat ggaaatctag cctcttctgt
    20341 tgctgtgcag tcttatggtg cgcaaccttc tatcccttac cgcgaagctt tagaacaatc
    20401 agcagtctaa aattaacaat gagaggaaga aacaaacatg aaaaaaggaa aagtaatcaa
    20461 ttcagatatt tcacgagtaa tcgcacaaat ggggcacttt gataaattaa gtatcgggga
    20521 tgcaggcatg cctgtcccct ttggtactga aaaaatagat ttggctgtct caaacggtct
    20581 tccaagtttt ttatctgttt tagaaaacgt tttagaagaa cttgaggtac aatcgattga
    20641 attggcggaa gaaataaaga caatgaaccc ggaaatattg gaaaaaatca aattattgtt
    20701 accagataca ccgattagct ttatcccaca tgaagaaatg aaaaaagagt tgaataactg
    20761 tcatgcattt attcgtacag gcgaaatgac accttacgcc aatattatat tagtgagtgg
    20821 agtggtcttc taatggaaac gacaatgaat cctgaacaga tggtactgtc cgagcagatt
    20881 catatgctga ttgaacagtt gggaggaaaa gacaatattg attctgtcct tccatgtgcg
    20941 actcggcttc gtatcaaatt gaaaaaacag ccagtagcca atgcagaaaa attaaaacag
    21001 caaatccctt ccattaagta tttcatttta ctcgatagat atgcagaaat aatcatggat
    21061 cagggattgg ctcctgctta caaggaattg acgcgtcaaa tcgatcattc agctccgaaa
    21121 gaaaagctaa atccgatcga aaaactattt ggtttgatat cggaaatctt tacgccgatc
    21181 attccgctgt ttgccggttc tggtatatta aaaggtctgg ttgttttagc gactacgatt
    21241 ggctggctta gtgaaacaag cggaacttat catattttgt ctgctgcgtc gaatgccgtc
    21301 ttttattttc tgccaatctt tcttgctttt tctgctgcca aaacgtttaa agtgaatgga
    21361 tatattgcgg ctgtcattgc tgcagcactg attgaaccga atctgacctc tttactttca
    21421 gacagtggca aagcagctac tgattttctg gggattcctg tagtactgat gcagtatacg
    21481 tctactgtca tgccggcgat tttaggtatt ttcttttatt cttttgttga aaaattcctc
    21541 aaaaaatata ttcgcgaaaa tatgcaatta gtatttgtac ctttactttc attagttatc
    21601 acggttcctt tgacgttaat ggtattcggg cctgcgggtg tttaccttgg cgaaggttta
    21661 gctgccgcaa ttacttggat gatggatatt aacggaccga tcagtggcgc aatcatcgct
    21721 ggtggttgga atattttggt aatatttggt atccaatggg ctgtaaaccc tgtcatgatc
    21781 agtaatatct ctgcttatgg gttcgaccga atcgttccgt tgacaggagc agccaatttt
    21841 ggaatggctg gtgcagcttt aggtgtattt ctgcgaagta aacgttcaaa aacccgttcg
    21901 atctccggtt ctgcttttgc ttcgatttta cttgcgggag tgaccgaacc aactgtttac
    21961 ggaattgcga tcccattgaa aaaaccattt gttgctgctt gcattggtgc agctgctggt
    22021 ggagctgtaa tgggatttgc ccaagtgaaa gcgatcgctt ttgtatttgg tagtctgacc
    22081 acattgcctg cattcatttc cggtactttt ttctggtact tagcaggact ggctgttagt
    22141 ctagtcgtag caatgatcac tactctcgtt agcgggtttg atgaagacct aatgtcttat
    22201 gaatagttaa tgaaaaagac tgaagtatga gatgtttatc acatatttca gtctttttct
    22261 ttttaggatc attattttta taaatttttg tgatactttc caaactttgg tataatttac
    22321 atatgcataa ccatttttat ccccacttgt ttatccccaa acaaaggtgg tctatgcaat
    22381 aaaatgaacg tcaaagaaaa acgaatgaat gctcgttgct tatttacaaa ttgttttatt
    22441 tagaatacac agacagattt atgaaaagaa aaagcttttc gacacgatta tctgttccaa
    22501 aaaatgtcta atgaatgctg aataagacag aaaatcaatg aaaggaaaag gactagttta
    22561 ttatgaaaaa taaaaaaacg atacgtttgc tagctgctac ttggctgatt gctccgtctg
    22621 ttttgtcgac caataccgta tttgccgtag ataatcaaat agctgatcaa acagtcacaa
    22681 cagatacgac tacatccagc tcagctacta gtgaaacaac ggattctttt acagaagacc
    22741 caactggaac aactaactca acaacaggat cgaccgagcc aactgaaatt gatccggctg
    22801 atccatcaga aaatgaaaag ccagccgaga catattccct tacaattgct ccttctttga
    22861 ttggtaagat ccaactgact gcttcgacag cggattcccc tgaacagcaa cctttggata
    22921 ttacggaaag tggaacggta gaaggtttat tagcggaaac gaaggtaact tacactatta
    22981 cgccagctga aggtgaacaa attgcttcag tcgctgttaa tggtgccaaa gaagtaaatt
    23041 atgatgcaaa gacctttaca gtaggtacga acaatttatc tattactgca gaatttaaag
    23101 ctggaccgga tacttcaaca gagaaaccaa ctgatcctag tgaagaagtc cctagtacac
    23161 cagatccgga aacgccaaac aaaccagatg tgggtaataa caacaataat aatggttcaa
    23221 ataattcaaa caataataac aataattcga acagcgggaa ttcttcgaat caaacaaacc
    23281 catctggatc gaataacaat ggaaatactc agagagaacc aaaccatcca aacaatggtt
    23341 catcgaatgg cggaacgaac aatgcttcat ctcgtccaaa tacagcaaat caaagcggta
    23401 tagagaatcc atcttctgct tcttctgatt ttgttgtaaa atcaccaatt gatgctgtat
    23461 tgcctacaaa tacaacgaat gtccaacaag caatcgttcg agaagcattc aagcatcttg
    23521 gaaaaccata tgtatgggga gcaaaaggtc caaatacttt tgactgttct ggtttgactt
    23581 attacgtata tatgaaagca accggtcatt atatcggtgg ctggacaggc gaacagcaat
    23641 atgcaggtac gcagattcca gtaagtcagg cccaacctgg cgacctcgtc ttctggggac
    23701 cttcttccgg tgttactcac catgtaggta tctatattgg gaacggacaa tttatccatg
    23761 cgcctcaacc aggagataaa gttcgagtaa cttctatttc agatttcacg cctgattttg
    23821 ctgtccgagt aaacttagca ggtttacctg ctgcttcagg ttcgctgaca ggcggctcat
    23881 ctattttaga tggattagat ggctcgttcc atttcagtca aaaccaaaca actgatcagt
    23941 tcttgaaaaa gattgcagat gatgcacagg aaatcgggca aaaagaagga atctacgcat
    24001 cagtcatgat ggctcaagct atccttgaaa gtggatctgg aaacagctta ttatcaagcg
    24061 aaccaaatca taaccttttt ggtatcaaag gatcatataa aggaagcagc gtgaccttca
    24121 atacacttga acaagatagt tccggacaaa gctaccaaat ccgcgcacaa ttccgtaaat
    24181 atccttctta caaggaaagt ttggaagact atgccgactt gatcaaaaat ggactgactg
    24241 gcaatcctga tttctacaaa ccaacttgga agtcggaaac aaaagactac aaagaagcaa
    24301 ccaaatattt ggaaggacgt tatgctacag atcgccaata ttcacaaaaa ttgaacgcga
    24361 tcatcgaagc gtacgacctg acaaaatatg atgaaccgaa aaaagaagca gcaacaactg
    24421 aagaagcaaa cgctgaaatc ctcaatatgt ctaagcgatt cgatgtgcca atcaaatgga
    24481 caacagatgg cgtgccaatc acaggattag aattttcaac gataaaacgc gtcaacaacg
    24541 cttcttccat cttccgtgtc gcttcaatct gggatttgtg gaacaatttt acggcaagac
    24601 agataccaga aacaactact cgtacagtta aagctgcaac tgttcctgta ctaaccttgt
    24661 taagtttaga caaatcattc tggcttgatc tgcctttaaa gaaatagaat aaattaaaaa
    24721 ggggatgtga cactgtcaca ctcccttttt aatttattct atttctttat atttattttg
    24781 tattccgtta caaactcgcg tacttggtta ggttccagag tgacatctcc aaaactttca
    24841 agtcgctccg ctcctggcag ttcttgagtc tcaaatgtga taccgccatg atttactaat
    24901 ttattcccgc gcatttccgg tccatcttct ccaaaatttg ctgtaaaaat cacgattcct
    24961 gaagcatctg tttttacttg gatcgatact ttttcatctg ggcttgtcaa ttccgcagca
    25021 tttagtaaac cagattgttt tttcaacaaa aatggatgat cgatgccgcc cactatcatt
    25081 gattggcgaa aatcactggc aaagacctca cctaattttt taggacgttg gaagtcaaat
    25141 gctgtgccac ttactgaaac tacttctcct gtaggcgttg tatctttatt gagcgtagca
    25201 aataatcggc tattcaacca aagtatatgt tcgtcgattg actgtgctgg atcaccggtt
    25261 aaattgaaat agacatgatt tgtaggattg aatactgttt tgcggtcact gatcccttgg
    25321 accgtcactc gccatgtatc atcgttcgtc agcgtatacc gtacttctac atctaaagtt
    25381 ccaggaaatc cgttttccat atctgggcta gtctgataga aaatgaccga tgcttcgttt
    25441 tcaccattga tgactgtata atgccactgc ttggcttcaa agctaggtgc accaccatgt
    25501 aaaccaatga ccagtgtccg ggtctgtttt tacttcgaac gtttcatcat ctaatttgaa
    25561 tgtgccattc gtaatccgtc cagccatacg tccgattgaa gcaccgatat aagggtcttt
    25621 ttctagatat tcttttgctt catcaaaacc taagatcaac tctcgttttt cattatcgat
    25681 tattgtttga aaatgaacaa cccgtgcccc aagatcactg acagacaaag atgtcccatt
    25741 ttgattcgtg atcgtaatca agtgatagtt ttcgccaaaa ttttgttcat tgatttccat
    25801 gttccttgca ccctcttttc tttcatttgc actgattcca gcatacatgg aaaccgtttt
    25861 cttaacagct tattccgtga gtaacgagaa aaaatttact atattctaat aaaagaccgt
    25921 gaaaaaaagg ttgtgacaaa aatattgtca caacctcttt ttccgaacaa acggtgttca
    25981 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgaggag ctatttttgc
    26041 ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctttt tcaatacgat
    26101 tccagtatat aaccatgtcg tcagtacata gtagatgaaa tacaatacga agaatatcgt
    26161 aaacggaacc caaatatctt tgtatggatt ttgcatgaat agaccaaaca tttgtaatcc
    26221 gaataagaca tggactacac ctaagattcc tggtgctaag aataacacgc ctacttctct
    26281 acggatcgat tgtttaagca acccgcgtcg tgttcctatc ttttcaagca tttcataacg
    26341 agcaatgtca ctcttgcttc ctgacaagat cttgaacatc aaacagctag caagcatggt
    26401 caagaaagct aaacctagga agaatcccat aaattcaaaa ccagagaata gaccattata
    26461 cacttcatac atttcatatt tctggttgct gtactctact ttttgcaaag atggattgtt
    26521 cattttattt tgagcaacaa gtgtttcgat tggtttcagg tttgcttgga agtcttttac
    26581 ctgaactagc tgtaaagtgg attgtggcaa gttcagttga tcaaattctg attgactgac
    26641 taccttgatt tctttttctt gctgttccgg caattcatag cttcgaagtt cctcttgtac
    26701 agtcagttca ttcatttttt tagcaggaac tttttctttc gtgatcttcg gcggtgttcc
    26761 atcttcatgt tttatcatga tcaatggttg agccgcaaat tcacttgcat tatagtaaat
    26821 cgttcccgca tcttcttttt gagtataaga agcagaaagt gttggtgaaa gttctttgat
    26881 tttttgctga tcttctgccc gaggattatt catcactaga tcatatccag aattgttttc
    26941 tgtcaagtct gcaatttcat ttttaaaacc taatcctacc gtcaaagcgc ccagtgccaa
    27001 ggcaaacagc atagcgacca ttgagagcat ttgtgtatat tcacgaatac gaaagtttaa
    27061 ctgtgacaat gtgaaattgt tcaatttttt caatgaaatc tgttctgatt tctttagtaa
    27121 tgataaaaag aaaatgatca cagaatggaa aatgaaataa gttcctaata cgatcgtaac
    27181 taaggctacc cctataccaa agacttgata ggtccccacg tgtttcatca tatagtaacc
    27241 gcagcctaga aaagcaattc ccagtactac ttcaaggaaa aacaagaatt tatgctgctt
    27301 gattcttgtt ggtgtctgat ccgcacgaat caatgctaaa atcggttttt tgacaatcga
    27361 aaaagcattg acaacagcag ctaataaaaa caaaacagca aaaaacaaaa cagtgatcaa
    27421 caacccgtga atactaaaag gtgtaaaatg cgaaatattg agatctaact gatccactaa
    27481 taatcggtgg acgatacttg tcaatcctac accgacagct gaacccacta aagtagctgc
    27541 gatccctact aagaatgttt cagaaaaaat catctgtgcg atttttctgc cttttgctcc
    27601 cagcatcata aacattgcat agtctttttg ccgcatcgtc atcaaaaagg aatttgcata
    27661 taaaatataa acgaatgtga tgatccctag taaaacagaa ccgaatcgga aaatgataac
    27721 tgtagccgca acgatcgtat tacttttcaa aaaagcttcg ttgcttgcca ttgattcaaa
    27781 catgtaaaaa atagcagatg ccataactaa tcctgagaat aacaccaagt agtctcttaa
    27841 acggctcttg atcccagtaa gtgataattt ccaaagcata gtcaatctcc ccctactgtt
    27901 ccaagtttcc aagaatagat agtatttctc gatagaagga atcacggctt tgatctccac
    27961 gtttcacttc ttgatggata ccgccatcct tgatgaataa aatacgttgg caataacttg
    28021 cagaaaacgg atcatgcgtc acaagaagaa tcgatacatg atcttttgta ttcaattcgt
    28081 ccatcgtatc taaaagatcc cgggcacttt tggaatccag cgcacctgta ggctcatccc
    28141 ctaatagaat cgttggttgt gtgatcaatg cacgtgcggc ggcgacccgt tgtttttgtc
    28201 caccggaaat ctcagaagga tagctttcca aaatatgaga gatggataaa cgttctgcaa
    28261 ttttttggac acgttgcttg atttcttttg gtctgacgcc ttgtaaagaa agaggaacag
    28321 cgatattttc ttgtgctgtc aagctttcta ataaattgaa atcttggaaa ataaagccaa
    28381 tctcattcgc acgaaaatct gctagttgat ttccttttaa tgtggtcaca tctttttgat
    28441 tgattcggat atgcccatct gttggtttat ctaatgtgga taaaatattc aaaagtgtcg
    28501 acttaccaga accagaagcg cccatgatcc caataaattc gcctttttct acatcgaagc
    28561 tgatgccgct taatgcttgc gtctttttct cattgttttt tccgtatact tttgttacat
    28621 tctgtacttc gactattttg ttcatttctt tccctaccct ttaattagtt tttccagtgc
    28681 tgttttcggc acgtcttttt tttcgacttc ttcatacgaa gtgacacctt tatttttgtt
    28741 ccaggtgatt tttaaatatg ctcctttacg aagcggtcga gtcaattgtc ccttgaagtc
    28801 cagttctttt tccttaccct ctttcgtata gccaggcagg atatatctat agttgactgt
    28861 ttcttgtcca gagtctgatt ttgaaactgt ttttgtacca tctgtcgtaa tttgtacata
    28921 gtattcttct ccacccataa ctacgtaatc tgcgattttc acccctgcaa gtaccacaac
    28981 gatgaaaaca agtagaccga tcagaagttt tttcactttt tccccttctc tcttacttta
    29041 tatactgatt atagaaaaaa gcgaacaatt ctgccttcga atcaactatc attcttcttt
    29101 caattttgtc attttcttta aaacaactaa aaaaagagtt caagacaata atccgtccta
    29161 aactccttct tcctatctta caaaaagtat gtcacttacg gtctttcatg tcgcctgtat
    29221 tgataacagg ttgcgttcct ttgtctgtaa tgattgagac aagtaaatta ttgatcaatt
    29281 ggactttccg atcatcggtg aaattgattt cttgtccatc ttcgatttgc tctaaggcca
    29341 tttgagtgat tgtgacagct ccttcaacaa tggtttgtcg agcagacaaa atggcttttg
    29401 cttgttgacg ttggagcatg gcactagcga tttctgttgc ataggctaaa tggttcaagc
    29461 gtgtttcgat tacttcgaca cctgctactg ctaaacgctc ttgcagctct ttttgcaact
    29521 cgtcagacac tgccgtcgtg tttcctcgaa gcgtcaaatc attctcattg aatgtatcat
    29581 acgggtattg gctagcgata tgccgaatgg ctgtctcact ttggatttct acaaaatctt
    29641 gataatacgc tacatcgaat aatgcctttg ctgtatcaac gactttaaag actactactg
    29701 ctgagatttc gatggggttt ccatccaaat cattgacttt taaaacagaa ctattgaaat
    29761 tccgaacttt caacgaaaca gtcatttttt gtgccaaagg tatcgtaagg aaaaagccat
    29821 tttctcgaat cgtacctaaa tattgtccaa aaaacaaaat tacttttgct tgattaggac
    29881 tgactacagt tgccgaactt aacaacaata acgtgatcaa ccaaagaaaa atacttaaaa
    29941 aaagtgccca aactttcgct tgccacatac ctaaatagaa gaaaaacaat ccaactaaag
    30001 ctaatacgac caatccgaca atccctgcat aaccattgat ataaaatgct cttttttctc
    30061 tcatgctact tcctgcgctc ctttatcctt caataaatat ctaatgttac ttttttaatt
    30121 tttttttcga atacgcgcca ttacaccttc tagttctaac tctttgtctt cagtgatgta
    30181 ccaaccgctt gcctgtgtcc ctccggctgc ctgcggtaaa taatccaatt ttgttcgaac
    30241 ccagtgcttt tcttctgtaa aatagtcttc taccaagacc tctagatcca tccctgctga
    30301 aagaggcaaa tcttcccgaa cagatttaaa gataaatcgt tccttcttcc catcatactt
    30361 caatattcct gtatcgataa tcggtaaaga ataataatcc agctctttct tcaccgtcaa
    30421 taaatgtttg aaaacttcat ggtacacttg ctctaattgg cgaccttctg aattgttcgg
    30481 gtaattaacc atcacttttt ctttgtctgg atcatagaag gtttggctta gttgagtgat
    30541 ataagtatgt aatctttcca tttctgtttt taacttttct ctttgacctt cttctacctt
    30601 catatactct ttcaccctgt tttcttttta gatattttct ttcttatttt actatagtta
    30661 tgtgcgttaa gcgatgaaaa acagcaaaga ttggaaggat ctttgctgtt ttttcttaaa
    30721 atatccagtt gagtacagga tcaatctgtt tcattgtcct cttgatccgt cgtaatattg
    30781 atcgatgtgt ctttagcagt gatctttagc tttttctgtc cttgttctaa caaagtattt
    30841 gttattggct ggtagtcttt ttcaatataa gatttaccag acgtttggat agtatagcca
    30901 ctgttttcca tctcatgcaa agataaattg ccaccgttta ggatgacttt tccttctttg
    30961 ctgatcaaac tgtcttctag atggaagttg ctgttttcac ctgtgacgat caagttatcc
    31021 acattggcct catctaatcg aagtgagcct aattcatctt tcgcagttat tgttcctcct
    31081 gtaaattcat tgaacgatac ccagccatca tgtgtcttag tattcacttt tttgattgat
    31141 acttcgttga tatcaattgc actattattc ccttcgattg tcaattcaga taattcatga
    31201 tctgcaggga caacaatagt gatttgtgca cttgtgtctt ctaaactaat gattgcacct
    31261 gttgattttt tgactgcatt cacagtcaac acgccatttt tctcctttat ttctggcttt
    31321 tctgtcttat cataagtcgt ttttatctca aaatccaatc cgcgtatgac cgaaactgtt
    31381 tgattttctg catggatgac gattttgttt gcatccttga ttttttttgt ctcagtaacc
    31441 atatcgatca accgaggacc attatcccaa gtcagactct tttgagcgcc taatgtataa
    31501 gttgcacctg ctaggaggcc accagtaagc atcagaccaa taccggtaat caaaaaatgt
    31561 ttcaatctca atttatgtgt cctccttcaa acgcccgctg aatgcagatc tctttttgta
    31621 ataaaacgtc gtcccatcca tttaacaaag ttcatcatga cataaaaaag cccttttgtt
    31681 cctttcagca acagtggcga aataaatact gcgcttcctg tggcgaacag accgacaccg
    31741 ataaagaata atccactcag aatcgattgt ccaaacacac tgattcctga gatgatcatg
    31801 aataggccaa cacctaagat actgatacat aaaacataga tactaaaaat gagcgctagt
    31861 aaagcaacca atagtccccc aataaccaat ataaacccca gtgctagtgg aatgaggaca
    31921 ggagaagcaa ataaaccgat aatgattaac caaatcaggc gaatttgtct ttttggattt
    31981 ttcactaact cgttttctgc ttgttcttct tctgaaaagt aactgatttt taattttaag
    32041 gcaaactgct ttggtgtacc atattcatca atgatctgat ctaatgtttt tccagaatct
    32101 aaaaaatatt cctcataata ctggatcaaa tcaaaacgat cgtcttcagg aatttcttga
    32161 agatgatcca caacttcatt caaatattca ttcatttgtg ctctcctcca ataaccggtc
    32221 taaagaatct cgaaaaacaa tccattcatc aataatttct tctaaatgac gacttcccat
    32281 tggtgtcaac gagtaatatc gacggtttct gccttcaaaa ggttcatcat aggtctcaag
    32341 atatccttct tttttcaatc ttctcaaaac cggatatagt gtagattcac tgacagagag
    32401 actttcttgt acgatttttg ttaaggcata cccatacata tcttccttgc ttaatgctgc
    32461 tagcacagta ccatcaagaa gaacagttgg aatctggatg tgcattacgt caccttctat
    32521 tctcaagtgt aatattttat ttcaatctat attatacaac atataatata gtttcaaaat
    32581 cagtttgtca aatacaaaaa aactgactaa aagatttgat cctttttagt cagcaaaaaa
    32641 tggcttctct ttgattttta ttataacagt ggcataatcc aatttgtcca catccagctc
    32701 agtggcatga taacgatcat tgcaggtatc atgtcagcta ctgggaacat tttcacgttg
    32761 atcattctaa aaccggtagc gaccatcaag aatccgccgc aagctttaaa atctaagatc
    32821 atgtctggtg tagtcaatgg ataaataaat tttgccaata agaacaaaat ataaaaaata
    32881 atgaattggg gaattgctat cacagaaacg acatacccta aattacacgc aaaaatagct
    32941 gctgtgaaaa aatcaaggac agatttagaa atcaaaatcg tactgtctcc tgtcatccca
    33001 gaatctaaac ttccatagat ccccgtacca cttgcacaaa atagaacaat gaccgtcact
    33061 aatgtgctga taaattcatc gtgggtcata ccaagctttt cactagggaa gattttagaa
    33121 ataggtactt gcatcagcgc agccccctta tttatcccat tacctaaatg aacaatcaaa
    33181 ccaatcgctg ttcctatcac taaagcaaaa ataacagccg gcatattttt catcggcgca
    33241 atggaataga ttcccatccc catcgaacac acaccaaaaa ccatcgtcag ctgtgcttta
    33301 aaatgttcac ttaatttatt tcctactaat ccgccaatta gtccgccaaa caaaacggaa
    33361 cctacattga tcaaaactcc agttggcatc aaaaaactcc tctctttttc tacgaagcga
    33421 aaaaaatcag aaaaatttct gtaattattg ctttcgctgc aagaaagatt gtaacagaca
    33481 gagaagatta caccaattca ttttttgtgt taaagtattc tttattggaa taagtaggag
    33541 gaatatgatg gatatccata aattcgaagt ttttcttgat ttagccgaaa caatgaattt
    33601 caccaaaact gctgatcgac aattcaccac ccaaggaaat atttctaaac agattctttc
    33661 gttagaaaca gaattagacg tcaaattatt cgaacgcgcc aatagaaaaa tcgaattgac
    33721 cgaagctgga agcttattgc tcccatatgt aaaaaatgtc gtagaacaat accatgctat
    33781 gcaagaagtg cttgaaacct atacgaaaga caacaatctt tcattaaata tcttaacgat
    33841 accgacaatg atcaattata aaggtttttc caaaatcacc gagtttttga aacgccatcc
    33901 tgaatttaca gtccagttga aagaagtgga aagtgtcgaa ttaacttaca atgacgccaa
    33961 tgaacatagt gacacgatcc actttgctag aagtttccag gccccaccaa gttcgattga
    34021 gtggttgcct actgaaacag acgattttgt agcagttcta cctaaaaatc atcctttagc
    34081 tagtaaaaag aaattggatc taagcgaact gaaaaaggag aactttttgc tccttggccc
    34141 cactacgaac ttgtatcaac ctgtactaga tctgtgtcgc gaagctggat ttgaaccaaa
    34201 aatctcttat aaaggagcaa gaatcgactt gatcatcaat atgattgcaa atgacatggg
    34261 gattgctatt gttatggaaa aaacagtcaa gaatctatta aaagaaaata ctgtgattct
    34321 ccctatttct ccaacaacag agagttatct tgcatttact cgtaaagtcg gtgagcattc
    34381 tttggcttca gatacatttt gggagtattt aaatcaagaa gaaaactaag ccctacattt
    34441 tctgtccatc tatccaccca aaaaaagaga gcgacgcatg actttcgctc tctttcttgc
    34501 atcatgtcct tgtttcaatg acacctctaa catttctcgt agttcaataa gaaagcagcg
    34561 atttttatgc caaaagaacc gtaagaacat ttccctaatc acaaaaaaac gactaaaaac
    34621 ccctaaaaac cttcaataga atttttaggg gttttattct ttttcctatt gtttacttaa
    34681 ccgtatcatt gctgtcgttc ctactccttc gatactgcta taatcaatac gtccatcatg
    34741 cagttctaga atttttttaa ccagtgaaag tcctagccca ttcccagaaa tttgacgact
    34801 tgtatctcct tgataaaatt tttcaaacat tcgatctctg gtctcttcat tcattcctac
    34861 acctgaatcg gtcacttcca gtacaatctc tgtggctgtc tcaaatagtt tgatatgaat
    34921 ctgtccattg atttgattgt actttatggc gttatccatg atattcagcc acacttggta
    34981 caaaaactct tcattgcctg tgtaatttac tgcagcaagt tcgatatcca attctagctg
    35041 ttctttctcc catttaggct gaaaaaaaac gattacttct ctgatttgtt catctaatcg
    35101 atactctttc ttttccagtt ggatacgctg gttttccagt ttgttcaatt ttaagatgtt
    35161 ctctgttaat tgtgaaagct gcgtgatact ttcgataatc cgatataaaa atatttggcg
    35221 ttcttcgtca gagagatttg gggcttgtaa caattgaaca tatccttgga ttgttgctag
    35281 tggtgttttg aattcatggg atacactgga cacaaagtca ttcctcagtg tctcgatact
    35341 attgagttct tggaccataa cgttgaaatc tttatacagt tgctgtactt ctgctacttt
    35401 ctgctgttca tccattcgaa tcgaaaaatc tcccgttgct accagactca tatttgtccc
    35461 tagcgcactg attggatgca ggatacgccg cccaacaaaa atcgatattc ctgtacccac
    35521 gaccatactg aaccccgcaa agacgaataa cgggtataac gttggttttc cagccgattc
    35581 agcaggcgag atagaatgtc gagacaataa gaaaataaaa ccgagaaaac acagcagtgt
    35641 cactaagatc gaaatgaaga caatactagc aaaatagatc cataattgtg aaaatagctt
    35701 ttttttcatc tttaaataac cgccttatag cctagtcccc gtactgtgat gatttgaaaa
    35761 tcttccgtct tttcaaaacg ggtccttaaa cgtttgatat gtacatcgat cgttcgttct
    35821 tctgtattcg tatctaatcc ccaaatatca tccatcaact gttggcgagt gaagatcttg
    35881 ttgggataag acagtaattt atacagaagc agaaattctt tttgaggcag aatcaacgaa
    35941 tgctgatctt gtaacactgt ataagcatct agctctaatt ttgtttgtcc gattatcaag
    36001 ctactgctgt aattgatctt tgaacgtcga agcaatgctt ccacacgtag gatcatctca
    36061 ttgacatcga tgggttttgt catataatca tctgctccta atttgaaccc gcgtttctta
    36121 tcctcaaaat cggcctttgc cgtaatcatt agaacaggtg tgtcaaatct ggtgctacgt
    36181 aatgagtcta gcaagtcata gccatccatt ttcggcatca tgatatctgt aatgattaat
    36241 tctatacgtg tcgcttctag ttttttcagc gcatcctctc cattcaatgc ggtgatgact
    36301 gaaaagcccg ctcgttccaa cacagaacgg taaagcaact gtaattgcgg atcatcttct
    36361 actactaaaa tacgattcat tgatctccct ctgttttttg aaaaatttct agattttttc
    36421 ttgtagttca tcttcagtta acatttattt cgtatactac gatcattgtt ctgatatact
    36481 tattagtata ccgaatattt caatacggga gaagggtgat tatgcttcaa ttaaaagata
    36541 taaaaaaata ttataaagtc ggcgaaacca ccaccaaagc attggatggt gtgtctgtcg
    36601 ctttcagaca aaaagaattc gtagcgattt taggtcctag tggttctggt aaaacgacta
    36661 tgctgaatgt gattggtgga ctagataatt atgattccgg tgatatggta atcaacggaa
    36721 agtctactaa agactttaag gacagtgact gggatgccta ccgaaacaac tctatcggtt
    36781 tcatttttca aagttacaac ttgattggtc atctaggcat tattgaaaac gttgaacttg
    36841 ggatgacatt aagtggtgtt tctaaagacg aaaaacgaaa acgggcagaa gatgcattgc
    36901 accgagtcgg cttgacaaat catatgcata aaaagccaaa tcagttatct ggtggacaaa
    36961 tgcagcgagt ggccatcgct cgagcactag ccaatgaccc agatatcctt ttgtgcgatg
    37021 aaccaaccgg agcgttagat acagaaacaa gtatccagat catggaattg atccaagagt
    37081 tatcgaaaga aaaattagtg atcatggtca cacataatcc tgaactggcc catcaatacg
    37141 cagatcgaat cattgaattt tctgatggta agatcttaac agattcccat cctcacatcg
    37201 aacgaccaaa agatgatcaa tttaacttac gcagaacaaa gatgagtttt tggacagcgt
    37261 taaagctttc cttcaacaat atccgaacaa aaaaaggacg tactttcttg acctcctttg
    37321 cttccagtat tgggatcatc ggaatcgcga tcgttctgtc cctttcttct ggattccaaa
    37381 aacaaatcga taacacgcaa gctgaaacga tggcgaaatt tccgattact atttctaagg
    37441 taacgactaa ccagactcgt gacgatgccg gattgggtgc aagcaaagcg gattatccag
    37501 acagcaaaac gattactgca aaagtcagcg aagaagataa agcacagcac acgaataaga
    37561 ttgatcaaac atacgtcgat tacgtaacag acatcgatcc taacttaagt aataatatcg
    37621 gatttacccg tactacagga atcaatcttt tacgtgatgt aaatggcaaa gtacagcctg
    37681 tcagcttctc gaatcaaaat cctgatacgg aatctctttc tttatctagt acgatgtcag
    37741 caatgacagg tgtgggagtg tcaagtttcc ctacacagct tgacactagc aaagaaaact
    37801 ttttaaaaga caattattct ttacttgccg gaagttatcc agcctcagca acagatgtcg
    37861 tgctgatcgt agacggcaat aataatacta atatcaacgc attgaagaat cttggatttg
    37921 atgtgaaaga agacgaaaaa cttgattttg atgatatcgt tggtacaaca ttcaaattag
    37981 taaacaacaa tacttactat acaaaacttc caactgggaa ttttatccct aatacagatt
    38041 atgatgcgat gtatcagaat gcttctgatg aattgaagat ctcggggatc ctacgcgtga
    38101 aatcttcttc tacaatgaat ctcttatctc caggaatcgc ttatagcgat caattaacga
    38161 ctcaaatcgt caacgaaaac aaagaatcag aaatcgtcaa agctcaaaga gacagtggtg
    38221 taaatgtctt aactactgaa aaagttgacg aaaatgccaa acaaacattg cttagttatt
    38281 taggtggaga cagcttgcct tcaagtatca tgatttatcc gaacaatttt gaagacaaag
    38341 aaaagatctt ggattatcta gatgattaca ataaaggcaa atcagatgaa gataaaatca
    38401 tttatactga tttagctgga acgatgactg aactgactgg cggattgatg gatgctatca
    38461 cttatgtcct tattgctttt gcagggatct ctttagtaac aagtatgatc atgatcagta
    38521 tcattactta tacatctgtg attgaacgga caaaagaaat cggtgtacta aaagctttag
    38581 gtgcacgtaa aaaagacatc actcgcgtat ttgacgcaga aacgtgtatt ttaggtattt
    38641 cttctgggat attaggtgtg tttattgcat ggctagctac gttccctatc aatagcatct
    38701 tatacaacat gacggatcta aaaaatgttg ctcaactaaa tcctgtccat gcgattattc
    38761 tagtgatcgt ctctactata ttgacgatgc ttggcggtca tcttcctgca cgaatggcag
    38821 cgaaaaaaga cgccgctatc gctttgcgtg cagaataagg ttgtgacaac cacatttaga
    38881 tttgagtcat aagagcgctg aaatagtaga aaacagcaag aaacgaggaa gtaatatgtt
    38941 ccttgtttcg cgctgttttt tttaattatt ttttataatt ctggtatact tttctttcct
    39001 ttgtaccaca actcttctgg cgtatacctg tcttttccat ttatgaatac tgttccatct
    39061 gcttcaatcg ttacttcagt cccaatccac atattacgag aaagtgcgag aagatttttt
    39121 ctttttcctc ttcctttttt tccaaattgc aaggcatcgg ttatctcttc tgtccaatga
    39181 gatttcacgt attttacttc tccgttttca aatgtaactt tcaaaatatt cgctatattt
    39241 tccactttta ctgctttagg aaatgactcc tccatttttt tcactttcct tcctccaaca
    39301 ctaagtatgc gctttctttt agtgataagt atatcatagt ttattctatt atcaaaaaaa
    39361 cgcatccatt ccattgtaga atactttacc gaacaagttg aattgttttc ctctttttca
    39421 ccttttacta tagataggta aagacaacta aggaggaatc tctcatgaca gatcaaccgt
    39481 atgatttaag aaaagtcatc gaagaactaa aaacacttcc aggtcaatat cacgaaacag
    39541 atattgaaat cgatcctgaa gcagaattat ctggtgttta ccgttatatc ggtgctggcg
    39601 gtacagtaaa acgtccgact caagaaggac caactatgac tttcaacaat atcaaaggtt
    39661 tccccggcac acgtgtgaac atcggaacaa tggctagtcg taaacgtgta ggtgaaattt
    39721 tgcaccatga ttatcgaaca cttggtcgtt tcttgaaaga cgctgtagaa aatccaatca
    39781 aacctgtgaa agtagacaaa gcagacgctc cagcacaaga agtcgttcat ctagctaccg
    39841 atgcagactt cgatattcgc aacattttgc ctgcacctac aaatactcct gaagatgctg
    39901 gtccatacat cacaatgggt gtagtcttag gatcagatcc agaaaaaaca atgagcgatg
    39961 taacgatcca tcgtatggtt ctagaagata aagatacgat cggtatgtac atcatgcctg
    40021 gcggacgcca cattggtcac ttccaaaaac aattcgaagc cttgaataaa ccaatgccta
    40081 tcactatcaa tattggttta gatccagcaa ttgccattgg cacgactttc gaaccaccta
    40141 ccactccact tggctacaat gaactatggt gtgcaggagc tttgcgtaac gaacctattc
    40201 aaatcgttga tggtatagca gtcgatgaag tgggtattgc tcgtgctgaa ttcatcattg
    40261 aagcagaaat catgccaaat gaaacaatgc aagaagatat caacacgaac tcaggaaaag
    40321 caatgccaga atttcctggc tatactgggg atgctaatcc agctgtacaa gtagtgaaag
    40381 tcaaagcagt tactcaccga aaagacaatc caatcatgca aacaacgatt ggtcccagtg
    40441 aagaacacgt atctatggcc ggtattccta cagaagcaag tattcttgcc ttggtcgata
    40501 aagctattcc aggaaaagta gtaaatgtct acaatcctcc tgcaggtggt ggtaaattga
    40561 tgaccatcat gcaaatccgt aaagaaaacg aagcggatga aggtattcag cgtcaagcag
    40621 ctattcttgc tttatcagcc ttcaaagaat tgaagacagt cattttagtc gatgaagatg
    40681 ttgatatttt cgacatgaac gatgtagttt ggactttcaa cactcgtttc caaggagata
    40741 aagatatcat cgtgatccca ggaatgagga accatccatt ggacccatca gaattaccag
    40801 aatataatcc tggctctatt cgtttccgtg ggataagtgc aaaaactatc tttgacggta
    40861 cagttccttt cgatatgaaa gaacattttg tacgtgcaaa attccaagaa gtagaagatt
    40921 gggaaaaata tttgaaataa tgaatgatct gcaactgttt tgacatcata aaaaggagtg
    40981 cggaaaatgg aacaattggc acaacggaag aaaaaaatcg tgattggtgt aagtggcgct
    41041 tcaggcacca tttatgccat caacctagtg aagaaactaa aagaatatcc gatgatagaa
    41101 acacacggaa tcatcagtcc atgggcaaaa cagaacttaa agctggaatc cgacttatca
    41161 ctaactgaat tcgaaagcct gtttgattac cattattcaa acaaagattt aggtgctgct
    41221 gtcgccagcg gatcattttt gaccgatgga atgatcatcg taccggcaag tatgaaaaca
    41281 gtcgcaggta tctctgttgg tttaggcgac aacctgattg cacgtgctgc tgatgtaaca
    41341 atgaaggagc aacgaaagtt agtcattgtt cctcgtgaaa cgccattaaa tgccattcac
    41401 ttggaaaact tgactaaatt agcacgacta ggtgtgcaaa tcataccacc aattcctgct
    41461 ttttataatc agcctcaaac gatccaagat cttgttaatc accatacaat gaaacttttg
    41521 gatcaatttg aaattgtaaa tgagcattca aaacgttggc agggtgatta atatggaaaa
    41581 agaaacgata gttgaagtaa ccaaactagg tttcacgatc caagctgctg ttaaagtaat
    41641 cggtaaagac ttattgatca ctttaacagg cggagataca ccacatatcg gaacagtcac
    41701 tacattttct cgtgatacag agatgcaaac tgtacgtttc cctagccacg atggtcggtt
    41761 ccataaagac gatgtcttag ccgaaaaaat agctgtgatc atcaaaactg ccgttccggg
    41821 aagttgtacg atcacttccg gtatccatgt tgaccatatc tccaatgcac aaatcaacgc
    41881 ctcttttccg atgtcagaag agttaggtta caagatttta gattggttgg aaaaaataga
    41941 tttccatatc aaagaaccag tttattataa agatggtgaa aaaccattat gaaagaagag
    42001 gttgtgaaat cagcgttatg acctgagtat gagagaaaaa aatggaaaaa tagcttctca
    42061 tattttgccg tttttgagct aataccgagg gacagctgat tgaacgccgt ttatcaagag
    42121 gttgtgataa agccgcttcg tttcgagtag taagaaaaat tttgtaaaca tgggtttcca
    42181 catttttgca taaatttgac ttactaccga gaaactggct tttgaacacc gtttatcaag
    42241 aggttgtgac acgtcttttg tcacaacctc ttttattttt gcttcactgt aactgctaca
    42301 atatcaccga tttcttttcc gatgttttta cgaatatctt ttcgtagtcc aaggatgtag
    42361 cagactgaac catctggatt tttcacgccc atattcacga tactgccttc atatggcaca
    42421 ttgtcaaaag ttgcttggac tttaactctt ccagcaccga attcttttct tatatcatac
    42481 ggaaaaataa tatatgcccc gcccttgccg acttcggaag catagatttt tgattcaaaa
    42541 gaataaattt ccctcatttt atttctccta ttttttacat ctagtatagc atagcaaaaa
    42601 aaagaaggaa ctgtgacaaa agtcttgtcg cagttccttt tttcgaataa acggtgttct
    42661 cctgccaact cctcggtcac aagtcacaat agtcaacaaa catgagaagc tgttttttga
    42721 aattgctcct tgtgactcag agttaaacgg caggctcaca acctcttcct taaacggtgt
    42781 tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg ttgttggaat
    42841 tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt tggattttca
    42901 gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc catcgtactt
    42961 tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa ttctacccat
    43021 gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt atcagcaata
    43081 ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt cgtcactttt
    43141 tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc agttgctttt
    43201 ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc attccctaaa
    43261 gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat tttttctgtt
    43321 tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac gatcaaactt
    43381 agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca tgttttttta
    43441 gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc aataatcatg
    43501 cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag gcaataataa
    43561 agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct gacagtgtgg
    43621 cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat ttgcttggga
    43681 tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca atcgagcttg
    43741 cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta tttggcaatg
    43801 cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac atacggatcg
    43861 cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca aatggtgcag
    43921 ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg tatttatcga
    43981 aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc aacaacatca
    44041 agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt acgaccattt
    44101 ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc gtgaatgtag
    44161 atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga cgccatgaga
    44221 acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag cttccccagc
    44281 cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc gttgataaaa
    44341 tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc attaggaaga
    44401 atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag tcacgccaat
    44461 ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca agtccaataa
    44521 cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata gatagcatga
    44581 tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa cgaccgatta
    44641 gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa aagatcgtca
    44701 tggcagaaag atataaattt cttgaactga atttattgat cagccatgct gttattggga
    44761 tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca gacgctgaaa
    44821 tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga ttcaaaacgg
    44881 tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat ggttttccgt
    44941 aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt tttctgaaac
    45001 tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac ataagttaat
    45061 acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt tggggcttcc
    45121 cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt aagtgatatc
    45181 gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat tcgttcgatc
    45241 gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa agtagaactg
    45301 atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa ggcagaaatc
    45361 cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa agatatcgaa
    45421 ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga gaatgctaag
    45481 atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct tcctgtcgat
    45541 gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat ctcctaagta
    45601 ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg tttgaacata
    45661 agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc catcactgtt
    45721 cggatatttt tttatttttt tcggttactt tcttctaata catctgttag ccaatttgca
    45781 tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta attttcatgt
    45841 ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag tcgtttttct
    45901 tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt cacgtctttg
    45961 acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt ggtgggtgta
    46021 tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac tttcttttcc
    46081 aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc catttttgca
    46141 agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc atttttgaac
    46201 gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa caaaatatat
    46261 cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta aatccattct
    46321 atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta taaggagatg
    46381 cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact ttatctatac
    46441 gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg ttgattaccg
    46501 tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg atatcgttaa
    46561 attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag acgtggcatt
    46621 ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta aatgggttga
    46681 agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa tggaagcagc
    46741 tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta cgatcactta
    46801 tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat acgatggaat
    46861 gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag taaaataagg
    46921 ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa agaaacggaa
    46981 aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
    47041 ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa cgctagttag
    47101 aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca gccgtttgaa
    47161 caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaatg
    47221 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
    47281 acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta gtgacttcaa
    47341 aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt attctgttca
    47401 tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta ctgcttcatc
    47461 tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag ctgattgatt
    47521 tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt tcattagttg
    47581 cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga taaactgatc
    47641 aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa tcactgcatc
    47701 tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg gaatgaattc
    47761 atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt ctttggcaga
    47821 caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga ttttaaaata
    47881 aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt atatcttctc
    47941 ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag ctgaaacgat
    48001 tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt cgctttgtac
    48061 tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa tgaatgaact
    48121 accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa gcactttttt
    48181 acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga ctacagctat
    48241 tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc cttttccttc
    48301 ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca aacgatgcgt
    48361 gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt ctgaaatcgt
    48421 tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat aagccaattt
    48481 ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta attcggtaaa
    48541 taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg aatttccacc
    48601 aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc taccattgag
    48661 ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc cgatcagcca
    48721 ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga aaggatcagt
    48781 accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt tctttcgctg
    48841 cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt gatcaataaa
    48901 actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta cgacgataca
    48961 tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac tctgcttcac
    49021 atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt caggaaaaaa
    49081 atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt aataagtaca
    49141 gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg cagatgcaag
    49201 cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg agtaataaga
    49261 gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt accgagaagc
    49321 agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt cgagtattaa
    49381 gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc ctctctcctt
    49441 agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa ttttctaatt
    49501 tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc aaaggagttc
    49561 gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg gcatttctca
    49621 tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc cattacaatc
    49681 ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt ccttcccttt
    49741 atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt ttcgcttatt
    49801 tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt ctttcaaatt
    49861 tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat gtcaatgttc
    49921 gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct attcaatact
    49981 ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta tgtatgatcc
    50041 ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct gtctcagtgg
    50101 gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg tatatccctt
    50161 tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt caactgatta
    50221 agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta gctattgttt
    50281 acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa aaggcgatag
    50341 atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg atattatcag
    50401 gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa atcgtgttgt
    50461 acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg acattgatcg
    50521 aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc gtgccattta
    50581 cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa caaaagtggt
    50641 tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc cagaacttat
    50701 accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact tatctattcg
    50761 atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc agcttgttcg
    50821 ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa gacaataaat
    50881 acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa acacatccag
    50941 gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat atccctgtta
    51001 tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat tatgaagtaa
    51061 cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc acgcttcatt
    51121 atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc tggtttacct
    51181 tgctatgttt atttatttat catagataca agcttttacg tgcttaggca aaagggataa
    51241 atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt tattttaatc
    51301 agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg atttccaccc
    51361 cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga tattttttaa
    51421 cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta ttttgttccc
    51481 aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg acaacatacg
    51541 aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag tctactggat
    51601 tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag ttttccaagt
    51661 ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag tggcagctcg
    51721 agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg cgtgtctgat
    51781 aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat gtttttttcg
    51841 taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt tcccaactcc
    51901 aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca aaattatctt
    51961 tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca ggttctactt
    52021 cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa tcttctaaca
    52081 ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc cagctatgcg
    52141 tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa caaaagggtt
    52201 gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta actcctcagt
    52261 aataagctca atggaccagg agctattttt tgtttggctg tttttatcac ttggtgcagg
    52321 gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct cggtaataag
    52381 cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat gacttgatgc
    52441 aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact ttaccagctg
    52501 tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta aatacgatca
    52561 tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca gctaatttcg
    52621 ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc tgattcattt
    52681 ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt tccagcccaa
    52741 ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca acaacttctc
    52801 ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat accggatctt
    52861 ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct tgttctatac
    52921 ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct ggctctggaa
    52981 tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct gcttttggtc
    53041 cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct gctcgtttcc
    53101 aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac cgagtcatac
    53161 aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc tggaccactt
    53221 gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg tcattgtcgt
    53281 aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa tttgctaaga
    53341 aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga tctccaccta
    53401 gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca aatgaatgga
    53461 cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact gaataaatga
    53521 catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt acacctgtta
    53581 gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc ttatcaggat
    53641 cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa ccaatcattt
    53701 gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat tgcgacatat
    53761 cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac ggatcttgcc
    53821 caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg ccaagctgtg
    53881 tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt aaacgttcta
    53941 atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct gcaatccata
    54001 aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga atcaatgcca
    54061 cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg aaaaatgaca
    54121 aagcatccgg caattttcta taattatagt acttattata agccattgcc ccgacaaatc
    54181 cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat acacttgtaa
    54241 aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca tcttcattcg
    54301 caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt aaaataaatg
    54361 agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct cctattgcta
    54421 aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca attactccgc
    54481 ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc cctatactga
    54541 tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt ccaaattttt
    54601 gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg caatcggttt
    54661 cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt ttatttttct
    54721 gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt ttctcctaaa
    54781 aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg acttgtgcaa
    54841 tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa atgaaacaga
    54901 tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta gataaaaaaa
    54961 atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg atgcgcggga
    55021 actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg ggtgtctggt
    55081 atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac tttggaaaag
    55141 tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag ctgatcgact
    55201 tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat ggtgtattat
    55261 ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc gaacgttttc
    55321 tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg cttgaaggta
    55381 gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa gacagcaact
    55441 atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc ggctatttga
    55501 cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact gcagcaatgc
    55561 gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact ttagcattac
    55621 atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa aaagaaatta
    55681 tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct gtctcagaat
    55741 taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg gcttgggcag
    55801 aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa caaggtattc
    55861 gattcaattt gttccaacta ttctctactt actacggcga agatgaacga ttgaatatcg
    55921 gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat acagaagcgt
    55981 atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat ctgctgaaat
    56041 atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga ttaaaaggcg
    56101 cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg gaaatcacat
    56161 tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat acgaattaca
    56221 ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa atcgcacgct
    56281 tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc cacggtgtga
    56341 ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat acgatcgcag
    56401 cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa gctgctatca
    56461 ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg tacttcccag
    56521 ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa gatctcatgc
    56581 ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct tgggacaaaa
    56641 tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat ttcttcaatg
    56701 atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca atgactgtcc
    56761 atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta ggaatggaaa
    56821 gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat tacaataacg
    56881 acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg attgtccaag
    56941 gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt ctgccaaaaa
    57001 cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc atctcagtca
    57061 cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt tacgtctatg
    57121 aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag gaagcaaatg
    57181 tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga attccattat
    57241 cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac attcaacgaa
    57301 cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta tggcggaaaa
    57361 gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa cgattattat
    57421 ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt gcctttactg
    57481 gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa aaacgggcca
    57541 ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga cccggcagaa
    57601 gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc tgtcggacta
    57661 aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc catcctctca
    57721 agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga gatcccgctt
    57781 gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca tgaccatgga
    57841 taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa tcacattgaa
    57901 gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc aaatcttcgc
    57961 accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt tcgaagatgt
    58021 tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa ggatccggga
    58081 tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata tccacggtgg
    58141 cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc catcatcaat
    58201 aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa tcactgctac
    58261 gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta gtagccttgt
    58321 tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa atggaacaag
    58381 agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat tagatcaaga
    58441 aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact ttagaaaatc
    58501 cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg tatttctttt
    58561 gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt ctggtcgcca
    58621 aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg gattcgaagc
    58681 tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa tcgaaccgca
    58741 agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc atcctggtca
    58801 agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga aaggtgtttt
    58861 tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca cctcgaccgt
    58921 ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga aagtccgaaa
    58981 aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag gaaaacgcat
    59041 ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga tcggtaaccc
    59101 attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg atatgactac
    59161 agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc gtatgcattt
    59221 acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata ctgtcattga
    59281 ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc atcatgagaa
    59341 cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa cagaattttt
    59401 gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg aaagtttgta
    59461 tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc ctgcgttatc
    59521 cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt tgaaagaaaa
    59581 cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga tgcagttggc
    59641 gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca acaactcgat
    59701 cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct ccgaattagg
    59761 acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca gtggcgtacg
    59821 tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag aaaacccata
    59881 ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct ttttatgtaa
    59941 aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg acaaaagtaa
    60001 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    60061 cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt tcttcttatt
    60121 tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt ttttacacgt
    60181 cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat cgagaaaaag
    60241 gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa tgtgccgctg
    60301 actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt tcgtgtcaaa
    60361 taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc tggaagaatg
    60421 atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa ggcagtaaac
    60481 ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat ggtatcaacg
    60541 atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa actagcagaa
    60601 cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg atcaggtgta
    60661 tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa acttttctca
    60721 tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg gatagccgga
    60781 atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca aggagagatc
    60841 caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc ggctgaatga
    60901 ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg gaaatccgta
    60961 taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc tgccaagttg
    61021 gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac gactgcgcct
    61081 gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc aattccaatc
    61141 gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca aacaagaaat
    61201 gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat aatcgtaggg
    61261 atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa aatcaggatt
    61321 ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc ccatgtgaac
    61381 tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg gatatgtggc
    61441 gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat caatacagcg
    61501 atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca aatgaaaagg
    61561 ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt
    61621 cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa
    61681 gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct
    61741 gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg
    61801 atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt
    61861 cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc
    61921 aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca
    61981 cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt
    62041 agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga
    62101 aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat
    62161 aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt
    62221 aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt
    62281 ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg
    62341 atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg
    62401 taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat
    62461 gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg
    62521 tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact
    62581 gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc
    62641 aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga
    62701 ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg
    62761 accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat
    62821 ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg
    62881 gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa
    62941 taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac
    63001 agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc
    63061 accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag
    63121 caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg
    63181 gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct
    63241 tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc
    63301 ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt
    63361 gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg
    63421 gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg
    63481 agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt
    63541 cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat
    63601 catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga
    63661 atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt
    63721 ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg
    63781 atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc
    63841 aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg
    63901 ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca
    63961 tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg
    64021 ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta
    64081 agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt
    64141 caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag
    64201 cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa
    64261 cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac
    64321 ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt
    64381 ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga
    64441 tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt
    64501 tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga
    64561 cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt
    64621 ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc
    64681 gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc
    64741 catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg
    64801 ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg
    64861 ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat
    64921 gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt
    64981 tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt
    65041 cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag
    65101 ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat
    65161 ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga
    65221 gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca
    65281 tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg
    65341 agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta
    65401 ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa
    65461 acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat
    65521 aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac
    65581 gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga
    65641 tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc
    65701 aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt
    65761 tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac
    65821 aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc
    65881 tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta
    65941 tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt
    66001 tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc
    66061 cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg
    66121 gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg
    66181 cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa
    66241 ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt
    66301 gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa
    66361 ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg
    66421 attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt
    66481 tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg
    66541 atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat
    66601 cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc
    66661 gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat
    66721 aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcatg
    66781 tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag
    66841 ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc
    66901 tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc
    66961 tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac
    67021 tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag agcgcaactt
    67081 tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc ttttttgaat
    67141 ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac tttttctgta
    67201 ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt cttcgttaca
    67261 ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag
    67321 caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg
    67381 ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca
    67441 gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg
    67501 gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga
    67561 tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac
    67621 tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa
    67681 aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa
    67741 ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa tttgaatgat
    67801 ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga
    67861 aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc
    67921 ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag
    67981 aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag
    68041 aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac
    68101 ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa
    68161 gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct
    68221 tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac
    68281 aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat
    68341 ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct
    68401 tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg
    68461 ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt
    68521 caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc
    68581 tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaatc gagtagaggg
    68641 gaaagccaac tgaccttccg cccctctcac accaccgtac gtacggaccc gtatacggcg
    68701 gttcaataag ttgagtgtac gaactggtac ttcaaggaga tgtcctcata tccttggcgg
    68761 gccagttctt tgtctgttaa tgatcgatga agaatatgac ttttggagac tgaccaaata
    68821 ccttttcttg tatttgacca ttcatacgct ttcgctttat cgatccctaa acgaatcaga
    68881 ttttttcttt tggttcttgg attcttccat tgtttccaaa tatattgtct gattcttcgc
    68941 ttcaaccaac cgtttaactc cctcatgaat tctttcatct caccaattcc atagtagttg
    69001 atccaaccag tcattagttg cttgatttct tttaaaataa gttggatact tcttccacgg
    69061 tttcttttag tgagttgttt gagcttgctt ttcacacgct gttttgcttt tgcgtgaggg
    69121 cgaattttaa tacctttctt tgttggcaga atgcagaatc caagaaactt tcgtttcaat
    69181 ggacttccta ccgcactctt ttctcgattt accgtcaact ttagttgttc ttcgagaaat
    69241 tgaatggatt tcttcatcac acgctgtcct gctcgtttgc ttctaacata gatattgcag
    69301 tcatcggcgt atcgaacaaa tttatgtcct agattttcta gcacttgatc aaattcgttc
    69361 aggtagatgt tacttaacag gggagagaga ttgcctcctt ggggcgttcc ttcttcagtt
    69421 gttgtgatga gtccattttc cattacacca ctcattaagt attgacggat cagttttagg
    69481 acttgtttgt cctttacttt ccgctcaatg aaatacatca gcttatcgtg atttacggtg
    69541 tcaaagtagg ctttcatatc aatgtccacc acatatcggt aaccttgttc ataataagat
    69601 tttgctttct tcactgcgtc atgtgcgctg cgatttgggc gaaacccata actattatct
    69661 gagaattctg gttcaaatag cggttgtaac acttgattga ttgcctgttg aagcatacga
    69721 tcaataaccg tgggaattcc tagttttctt acaccgccgt ttggtttcgg tatttctact
    69781 cgcaaaacag gctgaggttt atagttccct tggcgtaaat tggatagtaa ttcctcgcgg
    69841 tgctctttca agtaaggaag cagttgatcg acggtcatcc catctgcacc agccgcccct
    69901 ttatttctga ctacttgaag atacgcttga ttgagattat tccgatctag aattctccca
    69961 aacagatttt ctacaccatc ttgtttcctc tgttcaccgc aagaagtgct gcgcacttca
    70021 tcataccctt cgtgttccac actagctctt tgatgatagc caagttttct tgttttctgc
    70081 ggtcgtcgca tcttctcaca cctcctaaat tattcaagat tcttattgtt cagtccttcg
    70141 taacggtcag tgtcgttact actatgacct cggctgactt ctcatgattc accatcatgt
    70201 cgccatgatc gttgtgctgg tttgttttca ttcaacgccc acaacaccct catgagatct
    70261 cccccggtaa gagtgaaaac tttcctccca tgtaactgct agatttactg tataggtttc
    70321 gggcagtatt ggactttgtc ttgtgatgaa gacttatccg acctaattca gccttgtatc
    70381 tagtttctgt tcgtcagttc aggagtttgc gtccgacttc cttcagccac aacctcacga
    70441 ttgccgcctt gtctttcgct aacagttcct actgccaagc ctgtagtgga ctttcaccac
    70501 caagttttca cccatgcagg gcgcacacca aaaaagccga actgacattt tatcgtcagt
    70561 ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc
    70621 cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc
    70681 attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct
    70741 ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc
    70801 atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa
    70861 aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt
    70921 tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata
    70981 aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc
    71041 gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg
    71101 tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc
    71161 ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag
    71221 ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt
    71281 ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata
    71341 aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta
    71401 tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg
    71461 aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt
    71521 gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt
    71581 ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa
    71641 gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga
    71701 aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct
    71761 caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac
    71821 tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat
    71881 ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa
    71941 gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc
    72001 agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac actcgatgaa
    72061 gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa
    72121 gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac
    72181 ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa
    72241 ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat
    72301 tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt
    72361 tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct
    72421 ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga
    72481 ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg
    72541 aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt
    72601 ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa
    72661 cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct
    72721 gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag ttgtacgatg
    72781 atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg gctatctgta
    72841 ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt
    72901 tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca
    72961 ataacagatg tagctgattc ctccctttcg atgtggaaac ctttaggctg gtcttatttg
    73021 acctatccat ttacaactaa caactgaatt cctttgcttt atttgttttt gttcagtcta
    73081 atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc
    73141 cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta
    73201 ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg
    73261 gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa
    73321 cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc
    73381 gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa
    73441 gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat
    73501 tggaccaata tgttactagc tattctattt gctatattgg caatcctgat tagttctggt
    73561 gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg
    73621 tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc
    73681 tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt agcttactcc
    73741 tttgcactca attactttgg cggaattttg gatttaccag aatggttttc caaaacagct
    73801 gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg
    73861 ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac
    73921 ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca
    73981 aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa
    74041 tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt
    74101 ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg
    74161 gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc
    74221 gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct
    74281 caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga
    74341 aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact
    74401 catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta
    74461 aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga
    74521 tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat
    74581 gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa
    74641 caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc
    74701 taataaagaa acataacttc ccttattcca actgataatt ttcttttatc atagcataaa
    74761 cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt
    74821 tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg
    74881 aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct
    74941 tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt
    75001 cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata
    75061 gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg
    75121 tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc
    75181 atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact
    75241 ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat
    75301 agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag
    75361 attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt
    75421 cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa
    75481 tctctttcca tttttgccag tgttctttat attctttctt gatttgttca atcttatctg
    75541 ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat
    75601 ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat
    75661 tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca
    75721 gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat
    75781 cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc
    75841 gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg
    75901 ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac
    75961 tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat
    76021 acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac
    76081 ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc
    76141 accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg
    76201 atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg
    76261 gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc
    76321 ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc
    76381 cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt
    76441 ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt
    76501 cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa
    76561 aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag
    76621 tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt
    76681 tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc
    76741 aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac
    76801 agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag
    76861 ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac
    76921 aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat
    76981 atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc
    77041 catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc
    77101 actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc
    77161 accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg
    77221 tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac
    77281 acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat
    77341 ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc catcctttct
    77401 ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga
    77461 tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct
    77521 gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg
    77581 caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg
    77641 ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt
    77701 tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa
    77761 gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt
    77821 tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg gaaaatatgt
    77881 gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac
    77941 aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg
    78001 tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt
    78061 tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc
    78121 cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga
    78181 ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt
    78241 ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt
    78301 ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc
    78361 ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg
    78421 ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg
    78481 atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt
    78541 tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt
    78601 tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt
    78661 gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa
    78721 cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt
    78781 ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat
    78841 ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca
    78901 atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt
    78961 gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg
    79021 aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac
    79081 cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca ggttcagaag
    79141 tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta
    79201 acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt
    79261 attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg
    79321 gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa
    79381 aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa
    79441 caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac
    79501 aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa
    79561 actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat
    79621 taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt
    79681 ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt
    79741 gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt gtcgatatag
    79801 catcttcaac gtcacaggaa cttccatgaa aaagataatc tcaggagcaa aaacagataa
    79861 ccagtcattt aactgatgat cccaagcaat actgatttct tctgtcgctc gatcttgtct
    79921 gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc
    79981 ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa
    80041 ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt
    80101 gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc
    80161 atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc
    80221 aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag
    80281 attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc
    80341 gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt
    80401 atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac
    80461 actcaaggga acatctaccc attctttttg cttatgtttg tatataaaca cataacacca
    80521 gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaaatacat
    80581 aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga tcatttcagt
    80641 tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt tttctcttcg
    80701 gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg ttccgtatac
    80761 acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc aaaagattta
    80821 tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga ggtaatcagg
    80881 aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt cttttctgat
    80941 aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg gaaaaagacc
    81001 catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt ttgccaaatc
    81061 tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga tgcaacttct
    81121 tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg taggagttta
    81181 ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag aaaaagaaca
    81241 aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg ttctttttat
    81301 tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat tcctctagat
    81361 atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc aaatggatat
    81421 aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat ttggcagttt
    81481 tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc ctcttctgtt
    81541 tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc tagtattttc
    81601 aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg cggtcggatc
    81661 gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc tgtgattgct
    81721 ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg gtagtcgtcg
    81781 aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg attctcgtca
    81841 cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa ttgtgccagg
    81901 gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt taccttttca
    81961 acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg tgtgggttgc
    82021 caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg atgctcatcc
    82081 acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg taaaagtttc
    82141 cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc
    82201 aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt
    82261 ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg
    82321 atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg
    82381 actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga
    82441 tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat
    82501 acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa
    82561 agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt
    82621 aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg
    82681 gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag
    82741 gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg
    82801 attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct
    82861 tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg
    82921 accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta
    82981 ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt
    83041 ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg
    83101 atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag
    83161 ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata
    83221 ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct
    83281 aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct
    83341 tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga
    83401 tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc
    83461 ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct
    83521 ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa
    83581 agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc
    83641 tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc
    83701 atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt
    83761 ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg
    83821 caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc
    83881 tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc
    83941 cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt
    84001 atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa
    84061 gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga
    84121 tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt
    84181 ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct
    84241 tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc
    84301 aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac
    84361 cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat
    84421 gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa
    84481 aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc
    84541 ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt
    84601 ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat
    84661 caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc
    84721 tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat
    84781 atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg
    84841 aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg
    84901 accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt
    84961 tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt
    85021 ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac
    85081 atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt
    85141 agcagcagac attccacctg ctacacctcc gacaataacg attttcatct tatttccctc
    85201 ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag
    85261 atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga
    85321 cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc
    85381 cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt
    85441 ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat
    85501 tcattttctg cacctcattt acatttttat aaatacctca tggggtatat taaacaggag
    85561 ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca
    85621 taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga
    85681 ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc
    85741 aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc
    85801 gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg
    85861 agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga
    85921 ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa
    85981 aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg
    86041 attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa
    86101 ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat
    86161 atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta
    86221 gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca
    86281 gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc
    86341 gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag ccaaacaaaa
    86401 atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    86461 ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag aaaaaaacgg
    86521 gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    86581 caccgtttac caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc
    86641 aaaaacaact tctcctgttt ttggtttggt tggcttatta tcgagaagta gctactggga
    86701 gtctatttat caaaaaaagg aatgcgacat aggtcacacc cctttttt
//
LOCUS       DACEJR010000003        86420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEJR010000003 DACEJR010000000
VERSION     DACEJR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.003"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(552..2165)
                     /locus_tag="IW651_000183"
     CDS             complement(552..2165)
                     /locus_tag="IW651_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0812947.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="IW651_000184"
     CDS             complement(2306..3214)
                     /locus_tag="IW651_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0812948.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="IW651_000185"
     CDS             3397..4521
                     /locus_tag="IW651_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0812949.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4546..4725
                     /locus_tag="IW651_000186"
     CDS             4546..4725
                     /locus_tag="IW651_000186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812950.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4748..5665
                     /locus_tag="IW651_000187"
     CDS             4748..5665
                     /locus_tag="IW651_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0812951.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="IW651_000188"
     CDS             5658..6491
                     /locus_tag="IW651_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0812952.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="IW651_000189"
     CDS             6574..7692
                     /locus_tag="IW651_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0812953.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="IW651_000190"
     CDS             7686..9584
                     /locus_tag="IW651_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0812954.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="IW651_000191"
     CDS             9578..10801
                     /locus_tag="IW651_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0812955.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="IW651_000192"
     CDS             complement(10887..12821)
                     /locus_tag="IW651_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0812956.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="IW651_000193"
     CDS             13047..13703
                     /locus_tag="IW651_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0812957.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="IW651_000194"
     CDS             complement(13777..14130)
                     /locus_tag="IW651_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0812958.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="IW651_000195"
     CDS             14322..15251
                     /locus_tag="IW651_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0812959.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="IW651_000196"
     CDS             15262..16098
                     /locus_tag="IW651_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0812960.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="IW651_000197"
     CDS             16346..17122
                     /locus_tag="IW651_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0812961.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="IW651_000198"
     CDS             17298..18041
                     /locus_tag="IW651_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0812962.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="IW651_000199"
     CDS             18034..19614
                     /locus_tag="IW651_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0812963.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="IW651_000200"
     CDS             complement(19865..20344)
                     /locus_tag="IW651_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0812964.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="IW651_000201"
     CDS             complement(20360..21112)
                     /locus_tag="IW651_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0812965.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="IW651_000202"
     tRNA            21229..21299
                     /locus_tag="IW651_000202"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="IW651_000203"
     CDS             21660..22385
                     /locus_tag="IW651_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0812966.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="IW651_000204"
     CDS             22640..22906
                     /locus_tag="IW651_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0812967.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="IW651_000205"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="IW651_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0812968.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="IW651_000206"
     CDS             23359..23664
                     /locus_tag="IW651_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0812969.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24233..25069
                     /locus_tag="IW651_000207"
     CDS             24233..25069
                     /locus_tag="IW651_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0812970.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            25134..25565
                     /locus_tag="IW651_000208"
     CDS             25134..25565
                     /locus_tag="IW651_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0812971.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25633..26955)
                     /locus_tag="IW651_000209"
     CDS             complement(25633..26955)
                     /locus_tag="IW651_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0812972.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27109..28749)
                     /locus_tag="IW651_000210"
     CDS             complement(27109..28749)
                     /locus_tag="IW651_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0812973.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29079..29549
                     /locus_tag="IW651_000211"
     CDS             29079..29549
                     /locus_tag="IW651_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0812974.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30178..31281
                     /locus_tag="IW651_000212"
     CDS             30178..31281
                     /locus_tag="IW651_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0812975.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31565..32245
                     /locus_tag="IW651_000213"
     CDS             31565..32245
                     /locus_tag="IW651_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0812976.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32335..33369
                     /locus_tag="IW651_000214"
     CDS             32335..33369
                     /locus_tag="IW651_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0812977.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33383..33718
                     /locus_tag="IW651_000215"
     CDS             33383..33718
                     /locus_tag="IW651_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812978.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33702..36182
                     /locus_tag="IW651_000216"
     CDS             33702..36182
                     /locus_tag="IW651_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812979.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36238..37797
                     /locus_tag="IW651_000217"
     CDS             36238..37797
                     /locus_tag="IW651_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0812980.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37794..39158
                     /locus_tag="IW651_000218"
     CDS             37794..39158
                     /locus_tag="IW651_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0812981.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39329..40399
                     /locus_tag="IW651_000219"
     CDS             39329..40399
                     /locus_tag="IW651_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0812982.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40414..41082
                     /locus_tag="IW651_000220"
     CDS             40414..41082
                     /locus_tag="IW651_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0812983.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41102..41680
                     /locus_tag="IW651_000221"
     CDS             41102..41680
                     /locus_tag="IW651_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0812984.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42083..42994
                     /locus_tag="IW651_000222"
     CDS             42083..42994
                     /locus_tag="IW651_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0812985.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43250..44167)
                     /locus_tag="IW651_000223"
     CDS             complement(43250..44167)
                     /locus_tag="IW651_000223"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0812986.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44369..44806
                     /gene="zapA"
                     /locus_tag="IW651_000224"
     CDS             44369..44806
                     /gene="zapA"
                     /locus_tag="IW651_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0812987.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44864..45412
                     /locus_tag="IW651_000225"
     CDS             44864..45412
                     /locus_tag="IW651_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0812988.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            45527..47887
                     /locus_tag="IW651_000226"
     CDS             45527..47887
                     /locus_tag="IW651_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0812989.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47974..48288
                     /gene="trxA"
                     /locus_tag="IW651_000227"
     CDS             47974..48288
                     /gene="trxA"
                     /locus_tag="IW651_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0812990.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48470..50248
                     /gene="uvrC"
                     /locus_tag="IW651_000228"
     CDS             48470..50248
                     /gene="uvrC"
                     /locus_tag="IW651_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0812991.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50393..51292
                     /locus_tag="IW651_000229"
     CDS             50393..51292
                     /locus_tag="IW651_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0812992.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51285..52547
                     /locus_tag="IW651_000230"
     CDS             51285..52547
                     /locus_tag="IW651_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0812993.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            52710..54608
                     /locus_tag="IW651_000231"
     CDS             52710..54608
                     /locus_tag="IW651_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0812994.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(54907..55626)
                     /locus_tag="IW651_000232"
     CDS             complement(54907..55626)
                     /locus_tag="IW651_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0812995.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            55963..56295
                     /locus_tag="IW651_000233"
     CDS             55963..56295
                     /locus_tag="IW651_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0812996.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            56282..58285
                     /locus_tag="IW651_000234"
     CDS             56282..58285
                     /locus_tag="IW651_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0812997.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            58304..58774
                     /locus_tag="IW651_000235"
     CDS             58304..58774
                     /locus_tag="IW651_000235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0812998.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            58795..59379
                     /locus_tag="IW651_000236"
     CDS             58795..59379
                     /locus_tag="IW651_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0812999.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            59394..60380
                     /locus_tag="IW651_000237"
     CDS             59394..60380
                     /locus_tag="IW651_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0813000.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            60370..60681
                     /locus_tag="IW651_000238"
     CDS             60370..60681
                     /locus_tag="IW651_000238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0813001.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            60703..62484
                     /locus_tag="IW651_000239"
     CDS             60703..62484
                     /locus_tag="IW651_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0813002.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            62477..63853
                     /locus_tag="IW651_000240"
     CDS             62477..63853
                     /locus_tag="IW651_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0813003.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            63860..64495
                     /locus_tag="IW651_000241"
     CDS             63860..64495
                     /locus_tag="IW651_000241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0813004.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            64525..65880
                     /locus_tag="IW651_000242"
     CDS             64525..65880
                     /locus_tag="IW651_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0813005.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(66072..67466)
                     /locus_tag="IW651_000243"
     CDS             complement(66072..67466)
                     /locus_tag="IW651_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0813006.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            67558..68529
                     /locus_tag="IW651_000244"
     CDS             67558..68529
                     /locus_tag="IW651_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0813007.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            68526..69308
                     /locus_tag="IW651_000245"
     CDS             68526..69308
                     /locus_tag="IW651_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0813008.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(69477..69848)
                     /locus_tag="IW651_000246"
     CDS             complement(69477..69848)
                     /locus_tag="IW651_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0813009.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            69982..70425
                     /locus_tag="IW651_000247"
     CDS             69982..70425
                     /locus_tag="IW651_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0813010.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(70468..71277)
                     /gene="yidA"
                     /locus_tag="IW651_000248"
     CDS             complement(70468..71277)
                     /gene="yidA"
                     /locus_tag="IW651_000248"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0813011.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(71296..72663)
                     /locus_tag="IW651_000249"
     CDS             complement(71296..72663)
                     /locus_tag="IW651_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0813012.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(72647..73477)
                     /locus_tag="IW651_000250"
     CDS             complement(72647..73477)
                     /locus_tag="IW651_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0813013.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            73640..74077
                     /locus_tag="IW651_000251"
     CDS             73640..74077
                     /locus_tag="IW651_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0813014.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            74153..74773
                     /locus_tag="IW651_000252"
     CDS             74153..74773
                     /locus_tag="IW651_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0813015.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            74962..75468
                     /locus_tag="IW651_000253"
     CDS             74962..75468
                     /locus_tag="IW651_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0813016.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            75485..76384
                     /locus_tag="IW651_000254"
     CDS             75485..76384
                     /locus_tag="IW651_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0813017.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            76471..77355
                     /locus_tag="IW651_000255"
     CDS             76471..77355
                     /locus_tag="IW651_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0813018.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(77547..77618)
                     /locus_tag="IW651_000256"
     tRNA            complement(77547..77618)
                     /locus_tag="IW651_000256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77583..77585),aa:Arg,seq:ccg)
     gene            77678..79012
                     /gene="rpoN"
                     /locus_tag="IW651_000257"
     CDS             77678..79012
                     /gene="rpoN"
                     /locus_tag="IW651_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0813019.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            79215..80252
                     /locus_tag="IW651_000258"
     CDS             79215..80252
                     /locus_tag="IW651_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0813020.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            80293..81294
                     /gene="gap"
                     /locus_tag="IW651_000259"
     CDS             80293..81294
                     /gene="gap"
                     /locus_tag="IW651_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0813021.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            81404..82594
                     /locus_tag="IW651_000260"
     CDS             81404..82594
                     /locus_tag="IW651_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0813022.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            82698..83453
                     /locus_tag="IW651_000261"
     CDS             82698..83453
                     /locus_tag="IW651_000261"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0813023.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            83569..84867
                     /gene="eno"
                     /locus_tag="IW651_000262"
     CDS             83569..84867
                     /gene="eno"
                     /locus_tag="IW651_000262"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0813024.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            85267..86406
                     /locus_tag="IW651_000263"
     CDS             85267..86406
                     /locus_tag="IW651_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0813025.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        28459 a        14949 c        17826 g        25186 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg
    23701 attgtgacaa acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc
    23761 ccctcggcat tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc
    23821 taataatcag gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca
    23881 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt
    23941 ccttgtgact cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
    24001 cttgcatctg cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg
    24061 ctgttcttat tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa
    24121 tttccttaaa aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa
    24181 agaaaagagt aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg
    24241 tctgatttat tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg
    24301 tggaggcgtg ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat
    24361 ttctttttac tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt
    24421 atcaagcggg aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt
    24481 tggaggagta gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa
    24541 gcatgtgcag ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta
    24601 caccaagcat catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg
    24661 gggggttctg ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc
    24721 tttgttgatg ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac
    24781 gattttcttt tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg
    24841 ctttgattta tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg
    24901 agcaagattt agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt
    24961 cctcgtggtt cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga
    25021 atctagacta acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta
    25081 tactatgata aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac
    25141 gggaaatgcg tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt
    25201 atgatatacc attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga
    25261 aaaatttttt cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag
    25321 cggagtccta tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt
    25381 ctaaatatgc tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagcga
    25441 aaaaacgagg aataacgaca ataaggctta attctgcaga atacagagta gaagcacacc
    25501 aattttatga acacatcggt tatcattccg ataaaatgca aaaaagattt ttgaaaatcc
    25561 tgtaaatctt tatcaaaaaa atacccgatc accaagccga tacttgatga tcaggtattt
    25621 ttgttttttt gactaagaaa gtaattgcat caagaaacca gagatgatca aaacgatggc
    25681 accaccaagt cgattgccca tttgagcaaa agcaataagt tccatccgat tagctgcaga
    25741 taagacggct acatttcctg ttccgcccat cgagttgttg cataagccag cagtaatagt
    25801 tgattcaatt ggatataaac caaacaaacg accgacaaat cctgaaacaa ctgaaatcgc
    25861 gataacactt gtcagacata agacaacaaa ttgccatgtc aaagcagaag ctaatacatt
    25921 cagattcagc aacgcaatac cgataccggc aagaacagct gctgtcaagt ttttgacaat
    25981 caagttattg aacatgatag ctgcatcttc ataatactct ggtaataggt tggcgatttt
    26041 acagatgaca acgataataa tcataaatgc gtaagcatgg actttaggaa caaaatagtt
    26101 accaatttgt cctaagatga agaaagtcaa tgataccatc aaaccaacac ctaactgtgg
    26161 aatggaaggt ttcacagttc gaggttttcc atcgccaaga tcgccttttt ccattaattt
    26221 accattgccg ttgaattttg gcattgattg tccagcacga gcaactaatg ctgcaccgat
    26281 gatagctaag acatttgtca ttgctgtcgc aggaatcagg cgggaaatga tagaagaagc
    26341 atccgtacct aaaacatttg cataaatcgt tgataaagga acggctccgg ctccaacacc
    26401 accagccatc gcaggtaatg agatgtataa aacggaattt gcaaaaccat ttccgataag
    26461 catgcccacg agtcctacgg caaaaaagga gacgaccatt gaaataagtg caacgggaat
    26521 aaaacgaaca gaagctttca tcaatagatt acggttcata ccaaggatgc taccagtaat
    26581 caaggcagca atataaaagt ccaaaaagcc catattgctg acaaagtttt ctgtggattt
    26641 cactacatat tcaggtaaga tcccaaatgt agcaatcgca gcagaagcga agatcgcaaa
    26701 aacggaacca ccacccaaat aacttttgac gatagggatt tttgtcccga taaaatgaaa
    26761 taaattaccg aaaataacca aaactgcgat tggtccaatc atattcgtcg gtaatttccc
    26821 taaagccatt gcaatgatca ggacaaggac catgatcaaa taaagtggca aacttacgcc
    26881 ggtgattttt gtggaccaaa aaccttcttt tttcttttcc tcaactgcag ttgcagttgt
    26941 tgttttttgt gccattttta cagatccttt cttattctgt atcatatgaa aaaagccaaa
    27001 gaaaagcgac aaaagagcag gagttggctg gccgctgttt tgccgcagtc gcttttttct
    27061 ttttagacgt catacaaagt ttctgtatgt tcttttacta taacgatctt aatcttctaa
    27121 agatttatat tcaggaaccc atttagcaga ttcaactgct tctttgatat ccgtgatttc
    27181 ttcacgattc aagttttgtg cgacaactga ttttgcgaca gtttcagcaa tcgtttgaga
    27241 aaattcagtg atttttgcta ctggaggcaa gatagctgct cctggttttg ttacatcaac
    27301 gattccgcct aatgctcgac tagcttgaga aatgatttca gcatttactc gtgtagctgt
    27361 tgaagcaatc aaaccaagtc ctaaacctgg gtacatcaaa gcattatttg cttgaccgat
    27421 ttggtaagtt acaccattat attcaacatc agcagcaggg atacctgttc caactaatgc
    27481 ttttccgtcc gtccattcta ttaaatcttt tgcttttgct tctgccaatt ttgttggatt
    27541 agacaaaggc atgatgatcg gacgtggtgt atgagcagcc atttctttca cgatttcttc
    27601 agtgaatgct ccaggttggg tagaagtacc aatcatgatt gtcggatgga tcgcttttac
    27661 aactgcttct aagtttgtca attcatcagc attagtgaaa tctgagcgtt tgcgagcgaa
    27721 tgggatttga ccaggagtca atccttctgt atcttcaaac aataaacctt gtttatctac
    27781 ttgatagaaa tgttggcgtg cttcttcttc aggtacacct tgacggatca tctcatctaa
    27841 aaggatgttt gcgataccca caccagcagt accagcacca aatgtcaaaa ttatttgatc
    27901 tttcaattgt tcaccggata tattcaatcc gcctaataca cctgctagga caacaattcc
    27961 tgtaccttgg atatcgtcgt tgaaagtagt gatttgatct tcgtattttt ctaagatcgc
    28021 tgcagcattt cctcgtccaa aatcttccca atgcaataac aattctggga ataattcggt
    28081 agctgattga acgaattgat cgatgaattc atagtaagtt tcgccttcta cacgagcatg
    28141 acgattgcct aagtataagg gattattcaa taattcttca ttattagtac cagcgtcgat
    28201 cgatacaggc aaaacttgtg ctggattaat accagcagca gcagtataga ccatcaattt
    28261 gccgatagcg atgtcaacac cgttcacacc ccagtcgccc atacctaaga ttccttctgc
    28321 atcagtcaca acaatcaaac gaatgtctcg tccatctgcc gcgttcttca aacttgcttt
    28381 gatgttttct ggttcgtcga ttgaaagaaa tgcagcatct tgcggtttca agaaaatttc
    28441 gttgtattgt tcgattgaat ccgcaacgac aggatcataa acgacaggca tgaattcaac
    28501 taaatgttcg cccatcaatt tataaaacaa tgtccgattc gtattgaata gattcattaa
    28561 gaagatacgt ttttccaaat ctgtttgttt gcttaagtat tgaccataag cttgaacaga
    28621 ttgttgttcc agtgtttgga cagtacttgg aagcatacca gtgattccat attttgcccg
    28681 ttcttctttt gtaaaagctg ttcctttatt tagaaaagga ttatttaata agtttaaccc
    28741 tgtaagcatg aatgattcct cctaagtttt aatacaaacg aagtatagaa gtcagatgat
    28801 gatatagtca aaaagagcag gtatgataaa ttttatttat atgtaatata gccaaaagaa
    28861 atcaaaatag cagtatttgt tctttttata tcgacaatcc ttgcttgaat actatgcgta
    28921 tctcttatat gctagagaaa gaaaagtaga agaataaggg atagatggaa tggataaata
    28981 ggatttctga ggaaaaaagc acatgatagc gttgatagga gtcacctatg tcaggtgata
    29041 aaaatacata agtagaaaat taagaggagg aagagaaaat gaaagaagtt caattgatta
    29101 gaaaaagcga actatcagaa ggcggttgca atgcatgtgg cgtagtagaa gcgacaagct
    29161 atacattaaa attagattcc aatcaagcaa taatctctga actgacagta ggcggtcttg
    29221 tggacagtct ggcgcttgct gaaggtttta ctggagaaga tatttatgag atgttttcag
    29281 aagtccgtca gttaaaaaaa ggagaaaaat gcatagaggt tcatcatgag agcccgaatg
    29341 tgaggttcaa acgtggtgat aatgagatga tcttcaagaa tcatgtgtct gatcacactg
    29401 aactatatga aatcgtgaat caaatactgt ctgggatttt tgaattagga ccttatgagt
    29461 tcaaggaaga aaacggtaat ccaaaattaa atgaagaatg gcaagaaacg attgaaacac
    29521 aaagaaacaa tccacactta tttcaatagt cttccacagt atttcgtaaa aatgttgatg
    29581 aaaaagaaac accgtgaatc agaggtcgtg aaaaacctgc cctgcatcaa gtaataagaa
    29641 cagccaaaca aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgcagatgc
    29701 aagtttttga acaccgttta aggaagagac tgtgaagtca acgttctgac ctgagtatta
    29761 gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    29821 tcagctgatt gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag tttcgagtag
    29881 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac ttaataccga
    29941 gaaaatggct tttgaacacc gtttattcaa aaaaagggac tttgacacta cttttgtcaa
    30001 agtcccatcg gttttttatc tatttttatt acaatcattt cttccagagt agtgacatct
    30061 ggaatggctg aaatgtaaac agattatttg cctgtatcaa tgtcgaagag taatataaaa
    30121 agtgtaaacg ctatcatatt gttcatcatt caaattttat agagagaagg cattgagatg
    30181 gaaaaaacat atcaagcagg tacaaatgaa ggaatagtag attttatcaa tatggaagat
    30241 ttagaaatcg cggcttcaca agtgattcca gctggtggtt atggttacat cagcagtggt
    30301 gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatcg tttgattatt
    30361 cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga cgaagaaatg
    30421 ctgactgcac caatcatcat ggcaccagtt gctgctcatg gattagctca tgtgaaggcg
    30481 gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc aagttcttac
    30541 gcttcttgta ctttagaaga gataagagaa gcaggcgggg agaaagcacc gcaatggttt
    30601 caattttata tgagcaaaga taatggaatc aatttggata ttcttgaagt ggccaagcgt
    30661 aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa ccgtgaaaca
    30721 gaccgcagaa acgggtttac cttcccgctg ccaatgccaa tcgttcaggc ttatcaatca
    30781 ggagtaggcc aaacgatgga tgctgtatat aaatcttcca aacaaaaact tagtccaaaa
    30841 gatgtagaat ttatcgctgc tcattctgat ttgccagtct acgtgaaagg tgttcaatca
    30901 gaagaagatg tctatcgatc attagaatct ggtgctggtg gaatttgggt atctaaccat
    30961 ggtggccgtc aattagatgg ggggcctgct gcgtttgatt cgttgcaata tgtggcagaa
    31021 gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg acaacatgtt
    31081 ttcaaagcaa tcgcatcagg tgccgatctt gttgctattg gcagacccgt tatttatgga
    31141 ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa aacagaattg
    31201 gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa aataaaattg
    31261 cgtgagaatc gttttattta agaaataaat gacgaaaaaa ataggagtgc ggcagatgat
    31321 gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg aaacgaaaaa
    31381 ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat gtaaacgatt
    31441 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
    31501 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
    31561 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
    31621 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
    31681 accaacaaat accgttgagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
    31741 agatccggaa aatccagcaa ctggccagac aggttcttta acattggatg tagttcctga
    31801 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
    31861 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
    31921 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
    31981 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
    32041 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
    32101 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
    32161 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
    32221 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
    32281 actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat
    32341 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
    32401 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
    32461 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
    32521 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
    32581 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
    32641 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
    32701 gtatcagcac atgcagaaac aactatacct atagaagtga aaatcccagc aaaaagcttt
    32761 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
    32821 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
    32881 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
    32941 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
    33001 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
    33061 gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
    33121 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
    33181 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
    33241 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
    33301 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
    33361 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
    33421 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
    33481 tacattttat gatctaagtc cagttccgca tccaagtggt tcagtcaatg gcgcatcaaa
    33541 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
    33601 gaatgaagaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
    33661 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
    33721 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
    33781 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
    33841 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
    33901 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
    33961 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
    34021 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
    34081 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
    34141 tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca gcaggaagcc
    34201 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca gatattattg
    34261 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
    34321 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
    34381 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
    34441 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
    34501 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
    34561 caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca acaggaaaat
    34621 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
    34681 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
    34741 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
    34801 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
    34861 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
    34921 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
    34981 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
    35041 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
    35101 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
    35161 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
    35221 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
    35281 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
    35341 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
    35401 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
    35461 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
    35521 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
    35581 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctgtacaat
    35641 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
    35701 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
    35761 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
    35821 tgaagacaag ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
    35881 ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt aagaatgatc
    35941 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aaacaattga
    36001 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
    36061 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
    36121 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
    36181 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
    36241 agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt ggaacttttc
    36301 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
    36361 aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
    36421 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
    36481 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
    36541 tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
    36601 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
    36661 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
    36721 cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt ttggtttgga
    36781 cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
    36841 ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa atttgaaata
    36901 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
    36961 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
    37021 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
    37081 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
    37141 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
    37201 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
    37261 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
    37321 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
    37381 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
    37441 gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat caagcagtta
    37501 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
    37561 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
    37621 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
    37681 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
    37741 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
    37801 tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta agaaaacttc
    37861 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
    37921 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
    37981 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
    38041 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
    38101 cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat aaacaaattg
    38161 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
    38221 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg gaattagcag
    38281 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    38341 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    38401 tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    38461 ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct gcccaaactc
    38521 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    38581 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    38641 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    38701 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    38761 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    38821 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    38881 aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    38941 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    39001 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    39061 tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    39121 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    39181 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    39241 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    39301 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    39361 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    39421 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    39481 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    39541 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc agacacctgg
    39601 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    39661 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    39721 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag gagatacagt
    39781 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    39841 caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc gcttcgaaaa
    39901 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    39961 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    40021 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    40081 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    40141 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    40201 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    40261 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    40321 gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    40381 gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt taaagaagta
    40441 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    40501 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    40561 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    40621 gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga aattggcttt
    40681 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    40741 gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga gcagcttggc
    40801 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    40861 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    40921 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    40981 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    41041 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    41101 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    41161 gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa aagttgggat
    41221 cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    41281 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    41341 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    41401 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    41461 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    41521 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    41581 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    41641 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    41701 aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt tttttatata
    41761 ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt atatttttat
    41821 ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt aattgtttgt
    41881 agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa aaggagattt
    41941 caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc tacggttata
    42001 tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc cttagtctgg
    42061 atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat ttgcaatcat
    42121 tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag ttggacaaac
    42181 ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg atacagaaca
    42241 agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg actcgctaac
    42301 taccccactt gcaactataa acggcggaga gctggcggag tttctttctg aaacaaaaaa
    42361 tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg aagatggaac
    42421 caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag tgattgtatt
    42481 agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac aaacagaaga
    42541 aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg atgctggagc
    42601 aacggtttat ttgacccata ctacagatga attagtacag cttggagata tatgcgacta
    42661 cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta cggaatatgc
    42721 gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa cattagcaca
    42781 gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct ccactgggaa
    42841 ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg gttatatgaa
    42901 taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga ttgctgcaag
    42961 tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca aacaaaaata
    43021 gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt ttgaacaccg
    43081 tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc aatttcaaaa
    43141 aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg catgagaaca
    43201 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    43261 gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    43321 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    43381 atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa aagctggcac
    43441 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    43501 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    43561 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    43621 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    43681 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    43741 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    43801 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccaa
    43861 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    43921 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    43981 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    44041 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    44101 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    44161 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    44221 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    44281 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    44341 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcagt
    44401 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    44461 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    44521 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    44581 aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    44641 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    44701 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    44761 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    44821 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    44881 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    44941 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    45001 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    45061 attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag gaactgcttt
    45121 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    45181 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    45241 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    45301 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    45361 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    45421 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    45481 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    45541 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    45601 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    45661 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    45721 acctaaactt gaaaatatcc gtccccatat gaaacggatc gagatcggag cagacttgaa
    45781 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    45841 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    45901 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    45961 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    46021 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    46081 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    46141 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt tatttattga
    46201 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    46261 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    46321 catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    46381 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    46441 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    46501 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    46561 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    46621 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    46681 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    46741 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    46801 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    46861 aacaacgcat tatcctgaat tgaaagttta tggatacaat cgggcaaata cgattaatgc
    46921 cagcatggag ttcgatgtgg atacactaag tccaacgtat cgtttgctga ttggcgttcc
    46981 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    47041 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    47101 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    47161 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    47221 aaaagaaatg gaaaaagcaa agaaaaaagc aaacgaagtc gtgtcagagg cggaagaaaa
    47281 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    47341 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    47401 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    47461 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    47521 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    47581 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    47641 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    47701 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    47761 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    47821 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    47881 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    47941 taatcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    48001 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    48061 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    48121 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    48181 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    48241 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    48301 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    48361 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    48421 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    48481 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    48541 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    48601 ttttactggc agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    48661 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    48721 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    48781 aacgaatgaa aaatatcccc gtttggtgat tactcgtaaa gtactaaaag acaaagcact
    48841 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    48901 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    48961 tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    49021 cgttgatgaa gtgaaaaagt ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    49081 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    49141 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    49201 ggatcgagat gtatttggtt atgcgattga caaaggatgg atgtgtgtcc aagtgttttt
    49261 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    49321 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    49381 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    49441 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgaataagaa
    49501 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    49561 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    49621 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    49681 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    49741 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcacgagtga ttcgcgaagg
    49801 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    49861 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg caaaaaacga
    49921 taaacataaa acaaatgagt tgctattcgg tatagatttg gctgtagtac cgcttgagcg
    49981 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    50041 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    50101 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    50161 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    50221 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    50281 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    50341 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    50401 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    50461 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    50521 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    50581 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    50641 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    50701 aaaagagatt gaaccaaaaa tcgatttttg gatggaaaaa tttcaggtaa aagggaaaaa
    50761 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    50821 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    50881 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    50941 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    51001 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    51061 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    51121 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    51181 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    51241 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    51301 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    51361 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    51421 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    51481 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    51541 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    51601 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    51661 aactgactgg cttgcaatcg atgcttcgag caagtcaatt aggcatttca ccagaagaag
    51721 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    51781 gaaaaatgac agttggagaa gataactcga cagtccaata tgtcgtaagt tatgtagcaa
    51841 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    51901 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    51961 tttacggaaa actaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    52021 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    52081 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    52141 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    52201 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatatatgc
    52261 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    52321 ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca gtcggtatgg
    52381 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    52441 ctgcaaacat gtacaaatcg aatgtactcg tgtatagcga aggcggatta tggagttcat
    52501 tgaaacaatc gatttcaatt atgagaaatg aacgaaaaaa agcataaaat atagaaaaat
    52561 atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac ctatttctat
    52621 ttttttatta tattggatat atatattcat tgaaaataga gcataacaac tttataatgt
    52681 tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta ctttcagaga
    52741 agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca attttgcagc
    52801 ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct gcttttgatc
    52861 atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt ttctctgaac
    52921 aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca gaatatgtgt
    52981 tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt ttgatcgatc
    53041 ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga ttgccgaaag
    53101 aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac gggaaaaaag
    53161 actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa aaatttttaa
    53221 caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc gtgggtgata
    53281 tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat ttttcctctg
    53341 tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc ggctctgagg
    53401 catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt gaattagaga
    53461 aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc tatcagaaga
    53521 atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa gaagaattag
    53581 aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag ttgttgaatc
    53641 gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac tatgaggaat
    53701 caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt caaacgatcc
    53761 agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat agtttgatgt
    53821 attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc gaaaaagggg
    53881 gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct gtagatgaaa
    53941 aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa ctgctgggat
    54001 tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa gatttggcga
    54061 ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc aagagtaaaa
    54121 tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa aaaagcaatc
    54181 cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca gaattcgatg
    54241 caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta aaaaaaggag
    54301 agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta tccaaagctt
    54361 atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac ggtttccaaa
    54421 tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa tctgatcaaa
    54481 cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg aatacgacaa
    54541 aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat tatcgaaagg
    54601 acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac tgcaacaaaa
    54661 ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta gaagctattt
    54721 ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc ttccttaaac
    54781 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca tgagaagctg
    54841 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctaatcagaa
    54901 aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg aatctacacg
    54961 atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat ctttactttg
    55021 gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag gagaaagatc
    55081 tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa ggtcacttaa
    55141 taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag tgggctgttg
    55201 ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg ttccctccaa
    55261 cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc tttgtttcaa
    55321 tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt ccgtcagttt
    55381 tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg caagaatgag
    55441 tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg gcagaataag
    55501 aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat agcttacttg
    55561 atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc cgatgattgg
    55621 tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa aaatttaaac
    55681 tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg taaagagaat
    55741 tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat aataaaaagt
    55801 aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa
    55861 ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac atactttcaa
    55921 tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca agtgttagaa
    55981 gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg aaccctacgg
    56041 cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat ccaaacgaac
    56101 agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa gcaagaatta
    56161 gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac tttgaaagac
    56221 caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt gaaagaaact
    56281 tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa atcaggagag
    56341 gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt atcaaaatag
    56401 aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg ataaagaagc
    56461 aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc aattcataca
    56521 acaccacggc gatagccgtc aaaagaaact ccatttgaaa catcaagaac tttccttaca
    56581 agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag aagtccttga
    56641 tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag aagaggaaga
    56701 atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa gtaagacgac
    56761 catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg aagaactaag
    56821 taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag tttatctttc
    56881 gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga aatacgggtt
    56941 caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag aaaccaaaca
    57001 gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag gtcagtgcag
    57061 cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg cagaacggga
    57121 aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag gttgggtaga
    57181 tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag aagaagatat
    57241 atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc ctactaaatt
    57301 gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca gtctgccaaa
    57361 atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct tcttcgggat
    57421 gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac tagctcaaaa
    57481 actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga ttctctcggt
    57541 accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat taccaaaaga
    57601 actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg tcaatacgat
    57661 tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat tcgtggcagc
    57721 aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt ttgcctggca
    57781 agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa aaaatacagc
    57841 atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct taatcgtgcc
    57901 gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata atctttacgg
    57961 attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac taggtatttc
    58021 aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc cagccgctcg
    58081 attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt ttttgacact
    58141 gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg gaaaatttta
    58201 tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg tcaacatcaa
    58261 tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg attacttaat
    58321 tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga cgattttttc
    58381 agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag cgttaacaac
    58441 aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag gtacgcaagg
    58501 gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg atatgtcagt
    58561 tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag gtttgttttc
    58621 aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga aaaaacctga
    58681 acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga ttttaggttt
    58741 cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa gcagatgaac
    58801 gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag agcggcgtta
    58861 gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac gcaaatcgaa
    58921 acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa ataccgtcag
    58981 cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa gcaggaattt
    59041 ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga atctgaacaa
    59101 atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt aacattcatt
    59161 gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg gaacaatgaa
    59221 cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg ttttctgata
    59281 aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga tatccaagga
    59341 acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag tgtatggaat
    59401 accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc aatgagacat
    59461 ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg aaatccacga
    59521 tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat cttttaaagg
    59581 agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa atcgtttgga
    59641 tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc gagatgacaa
    59701 atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa accttgaagg
    59761 atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac agtatacgag
    59821 aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg atttatgatc
    59881 gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa gaattgctga
    59941 cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga ggaatattgc
    60001 agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt atcgaaaaag
    60061 aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt ctattagaga
    60121 cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat ctcgtaaagc
    60181 ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa acccaggctt
    60241 tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc
    60301 gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga aagcgggtga
    60361 gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt tttgccattc
    60421 aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt
    60481 gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg tgcagcattg
    60541 agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt attgattccc
    60601 aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt agaaaaagca
    60661 gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc
    60721 ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag tatccaagac
    60781 atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg tggcgatgtg
    60841 gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc ggttcgctct
    60901 actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat gtttgacgga
    60961 atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt agggcgcgga
    61021 gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc
    61081 ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa agttataaaa
    61141 cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga aaaaggttca
    61201 ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg
    61261 atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt aaacccggat
    61321 gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac aaaaggagga
    61381 gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca tcaaattgca
    61441 aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg
    61501 acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga atcgttgatg
    61561 gaacgtacgg tattgattgc taatacatca aacatgccag tggcagctcg agaagcttct
    61621 atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga cgttgctatc
    61681 atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg acgtttggaa
    61741 gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc ggaatactat
    61801 gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag tataaccgca
    61861 atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca aaatacattg
    61921 cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag acattttcct
    61981 tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa gtatatggat
    62041 catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa
    62101 gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc agataatgac
    62161 cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca aaatgctttt
    62221 gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa gatgatcttg
    62281 acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg
    62341 gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc ggaaacagaa
    62401 ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca
    62461 gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt
    62521 gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca
    62581 aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag caatggtcca
    62641 aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat ttttaggcca
    62701 tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg gtttgggacg
    62761 accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta acggagaagt
    62821 aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta tctctgcaat
    62881 cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg gctctggtct
    62941 tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg attcatcaga
    63001 tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg aatttttcat
    63061 ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga atttagcaaa
    63121 cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg cagaatatct
    63181 tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca actatgcaga
    63241 ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag gatatcctgg
    63301 gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc gcggactaaa
    63361 aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa ctcatccaat
    63421 ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag agctttacaa
    63481 gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa aagataaggg
    63541 aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc tttttgcagc
    63601 ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat ctgctttatc
    63661 tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca
    63721 aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg aactgctatc
    63781 gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca aatatctgcc
    63841 agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca tggaattgac
    63901 tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa aagataagca
    63961 agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga
    64021 aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg cttcgatcac
    64081 ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt tgtctatcgt
    64141 agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg atgaacagct
    64201 gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg atcggtcaat
    64261 cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg aaaaaacttg
    64321 tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat tagaatatat
    64381 gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg aagaaaacga
    64441 gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg actaagtggt
    64501 atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa gaaacgtttg
    64561 ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg cggcggccta
    64621 cttaccctac cattttttag taatagtggg catgggacaa attttatcga tgcgcttttc
    64681 actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc agagcattgg
    64741 aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct tggttttatg
    64801 atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag cacacgtatt
    64861 atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct catgctttac
    64921 atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc ttttgtattt
    64981 gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc aatctctagt
    65041 ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca gcaaaatgtt
    65101 tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt catcgtatgg
    65161 cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa aatcgcatta
    65221 accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac agagcgcaat
    65281 ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt ttttatgagt
    65341 gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag ccatgcaggt
    65401 ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac ggccggcgga
    65461 ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt gaaaggacgt
    65521 acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact
    65581 ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc ggtgactgaa
    65641 acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc tgcgtttggt
    65701 acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa attgatcatt
    65761 atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc tctgatgaca
    65821 aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat gattggataa
    65881 tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa taatttttct
    65941 tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac tacccgttgt
    66001 tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg attcctttat
    66061 ttatcaatag gttataattc atattttttt attttttctt ttacgatact tagtccagaa
    66121 gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc tggttgcgca
    66181 cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa gtcattctca
    66241 attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg gacacgattc
    66301 gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt tgtaagactt
    66361 aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt gatgacttcc
    66421 acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat cttgctttgg
    66481 acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa ttttgtcatt
    66541 ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat gatcgtatgt
    66601 tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag accttctttt
    66661 tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc tataggatta
    66721 ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc ctgaatcaat
    66781 atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa atgatataac
    66841 tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct atcacctgga
    66901 gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac aatagataga
    66961 gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt agtcaattga
    67021 aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg gttattacta
    67081 gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact aaccagacca
    67141 aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc aaagacattg
    67201 ggagaattaa atgtgccact tccaatattc gtatacagca aacctttctc aattccatat
    67261 ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag ctctctttgg
    67321 gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc taattgttga
    67381 atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca agttaacggg
    67441 taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat accatacaat
    67501 tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga attgtctatg
    67561 aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc gattcaagta
    67621 atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg
    67681 gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc ttatttgggg
    67741 gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca tcagtatcat
    67801 ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact acccaaagca
    67861 ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc gctggcaaca
    67921 gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg tcaacaagca
    67981 ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt tgtttacgat
    68041 attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta tcctaaaata
    68101 acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat catttgtacg
    68161 ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga cgcacatatc
    68221 aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt gatggaaact
    68281 tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga cctgcttata
    68341 gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg cgaattagtt
    68401 tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga cgcagttggt
    68461 ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga gaaaggggct
    68521 ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa
    68581 atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg caaaaaattg
    68641 ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc
    68701 ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag
    68761 catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt tctactcaaa
    68821 gcggtaatct atttgtgaca agaattaaag attattatta catggatttt cctagtatta
    68881 tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct aagattaaag
    68941 aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg gtggctaaat
    69001 tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc atcgtgacag
    69061 cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta acaatcaatg
    69121 aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca gaaagaatga
    69181 ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta gaatgtgaat
    69241 taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa ggtacctcgt
    69301 ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca accttatagc
    69361 tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt ttgcgtctca
    69421 ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca tactaattat
    69481 cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta agaaggcgca
    69541 gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg ttgctcgtgt
    69601 aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc tagatcatac
    69661 gcaccatgat catagttgta agtcaactct aattcatagt catccccagg aagtgtcaaa
    69721 taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat
    69781 gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca tgtgtgagcc
    69841 attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa gaagtaagga
    69901 aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata gaaaataaaa
    69961 ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa gcaatttgct
    70021 gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca gattgcagta
    70081 atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg agaagagacg
    70141 aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc agaaatttct
    70201 tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac ttattattat
    70261 aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc aactgaaaag
    70321 accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc
    70381 caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa agcgagtaga
    70441 tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg cttcagctac
    70501 accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa gagaaacggc
    70561 atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt tctcgttatc
    70621 tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc
    70681 cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt
    70741 tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg
    70801 ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt caggtgttcg
    70861 atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg
    70921 attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga cacggcgtgc
    70981 catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga tgattttacc
    71041 tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat tatcttgaaa
    71101 aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag tgcataaaac
    71161 gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt ttgttacttc
    71221 tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga tagacataat
    71281 ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat ggctggcaaa
    71341 ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat caaccatttc
    71401 ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag caacaagctg
    71461 actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg
    71521 atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag aattcacagc
    71581 agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt cttggactaa
    71641 ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg
    71701 tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa agtaagtgcc
    71761 caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt caagaaatgg
    71821 cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca actcgtgtgc
    71881 gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat ggaagtacac
    71941 ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca tgccactcat
    72001 ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg taagatcaaa
    72061 tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca ttcgatctgc
    72121 atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt tttggatcac
    72181 actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat acacttctgt
    72241 ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga agatatgttc
    72301 aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac ataaagtcac
    72361 cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa aatttcgaga
    72421 aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag taaaacggct
    72481 atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc gtcgtagacg
    72541 ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat ggatatagtt
    72601 atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat aaggggttgt
    72661 cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg ttgttccatt
    72721 gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca cttgcagaat
    72781 acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa aggggttggt
    72841 caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag ccatttgtac
    72901 caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg cgtttttcct
    72961 gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc cgacgctgag
    73021 cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga caataagaat
    73081 gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc aataggagca
    73141 tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg aaaatcaaag
    73201 aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt ctgatcactg
    73261 gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga gttcgaacat
    73321 ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag atttgctggt
    73381 gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag tcgttttttt
    73441 gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct ttgtataaat
    73501 tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa aaaggttttc
    73561 tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg tagctatgag
    73621 aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa tcaaattgcg
    73681 tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga aagggcaaat
    73741 ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg agacgggcga
    73801 tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga tccgtgcagg
    73861 agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat cctatcgaac
    73921 accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga
    73981 ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat
    74041 cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa gtttgatttt
    74101 ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct gattggaaat
    74161 tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag cacacgcgat
    74221 cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga tccaaaacta
    74281 tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata ctgacttgaa
    74341 tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt catggtatgc
    74401 tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg aagatactcc
    74461 acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag acgcgatcga
    74521 ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg atgatctgtt
    74581 tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact tgcaagaaat
    74641 cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg aagaagattt
    74701 aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat tttctgactc
    74761 tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat cagcctatca
    74821 gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata aaaatcccaa
    74881 gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg tcatattaag
    74941 tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg gcagatcgaa
    75001 aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat gttccgtatc
    75061 acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga tagttttcat
    75121 aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc atggttttat
    75181 cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac gcctgtggac
    75241 ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt tgcagagctc
    75301 catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct ctactttttt
    75361 gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa cacagttaaa
    75421 acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag gagagaagag
    75481 tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata aagaacaagc
    75541 aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag agcaatcatt
    75601 tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt tggctatcgt
    75661 aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg cagaagattt
    75721 acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg atcgatctga
    75781 aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat
    75841 tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa aagctgaaaa
    75901 agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc agaaaatcgt
    75961 agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag cagatagcca
    76021 tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct cacttccact
    76081 tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac ggcagatggc
    76141 cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata ttcaagaagg
    76201 gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga acaaagagca
    76261 tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg gtacaccaga
    76321 atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt atttggatca
    76381 ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca agtaaataat
    76441 aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt tatcagtatt
    76501 attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt gatcaccgaa
    76561 gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa
    76621 tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat tcaagaatca
    76681 gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa aacgagccga
    76741 gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc gaccacaaca
    76801 ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga
    76861 gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt
    76921 ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc gatgacggag
    76981 gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc cacttttgtt
    77041 cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg accgatcatc
    77101 gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat tttgcagaag
    77161 atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta tcctgaaacg
    77221 attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg catcacgaca
    77281 gttggggatt tattagggca tccaacggct gtcgagaact attatggcag aaacgatcat
    77341 cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta ataagtagtt
    77401 gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta caacaaaaaa
    77461 ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag caactattcg
    77521 taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga
    77581 accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat aattgtaact
    77641 aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc aaaatttttc
    77701 acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac agtccatcca
    77761 agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag caatggaaaa
    77821 tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag cttcttcatc
    77881 ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa aagggtcttt
    77941 atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt tgaggaaaat
    78001 cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta gtttagaaga
    78061 agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga ctctgattca
    78121 gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac
    78181 agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat gttttgagga
    78241 actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc tacatgctgt
    78301 tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga ttttcgaccg
    78361 tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt
    78421 acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga gttacttcaa
    78481 acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac ggaaacagga
    78541 attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc gggttgctca
    78601 agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa tcaagcctct
    78661 tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt
    78721 caacgggaaa tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat ttttcactac
    78781 acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata cagcgaagaa
    78841 aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtctg accagaaatt
    78901 ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag caaaatatcg
    78961 tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact aatcagtcaa
    79021 aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg agagcaaaac
    79081 tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat tcgaatagtt
    79141 aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta aagtgtccaa
    79201 ctaaggagat cgctatgttg gatgaaataa aaatgattga atctgttgca ccagatatta
    79261 tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg cagccgattg
    79321 gacgtcgaag tttatcggaa agtatgggga tcacagaacg agtattgcga acagaaacag
    79381 atcttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta
    79441 agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta ggtacgcatc
    79501 aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa
    79561 acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc gaagccctag
    79621 accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact actatggctg
    79681 tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg tttgtccctg
    79741 caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg
    79801 ctgctaaagc aaacggtaac caccgtgcac tttatgttcc ggaacaatta agtctggcaa
    79861 cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg attagtgaag
    79921 cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt cgtaagatga
    79981 ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct tttggctact
    80041 ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata gaattgcaag
    80101 atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa gccaaagcaa
    80161 ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat gaagctgctg
    80221 caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat tttcataagg
    80281 aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat tggacgtcta
    80341 gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa cgacttgaca
    80401 gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga
    80461 acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa agtattagct
    80521 aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt tctagaatgt
    80581 actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg tgctaaacgc
    80641 gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa cacaaaccac
    80701 gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac taactgttta
    80761 gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt aatgacaact
    80821 atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa aggtgacttc
    80881 cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc tgctaaagct
    80941 atcggtttag taattcctga attgaacggt aaattagacg gagctgctca acgtgttcct
    81001 gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt aactgttgac
    81061 gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa cacagacgaa
    81121 atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc tacacaaact
    81181 aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta cgacaacgaa
    81241 atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt ataagataga
    81301 caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc aatcgcgctt
    81361 cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta agaaaacagt
    81421 gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca acgttccttt
    81481 gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa caatcaacta
    81541 cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg taaaaacaga
    81601 agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg aattattagg
    81661 taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag caatccgcaa
    81721 catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata ttgacggtaa
    81781 aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag gcgatgtatt
    81841 tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag gtatcgcttc
    81901 aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt tcatcggtga
    81961 agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa aagtttctga
    82021 taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga tcggtggcgg
    82081 aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc tagtagaagc
    82141 tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac ttgtcttgcc
    82201 aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag taatcgaagg
    82261 agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa aattatttgc
    82321 tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg tatttgaaat
    82381 gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact tagaaggcgc
    82441 aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag gatttgctga
    82501 caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg aaggtaaaga
    82561 attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc aattctgacg
    82621 agtaatcgat caaaaggtga agggagtttt catgactccc tagccatttt tcaataaatt
    82681 aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa atgaacaaaa
    82741 ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct tcaaatgaca
    82801 aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg acatcagctg
    82861 aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca ggtgcgttca
    82921 ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg atcatcggac
    82981 attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa aaagcgaaag
    83041 ctatctttgc aaatggtatg acaccaatcc tatgctgtgg cgaatcttta gaaacctatg
    83101 aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta gctgatcttt
    83161 cagctgaaca agtgtcaaac atggttattg cttatgaacc aatctgggca atcggtactg
    83221 gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt caaacagttg
    83281 aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc ggttctgtaa
    83341 aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct ttagtcggtg
    83401 gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa taattgttga
    83461 aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact aaaatcgtat
    83521 atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat gtcaattatt
    83581 actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat cgaagtagaa
    83641 gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc ttcaactggt
    83701 gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg taaaggtgta
    83761 actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg ctacgatgtt
    83821 cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc taacaaaggt
    83881 aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc agctgctgac
    83941 tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt attgccaaca
    84001 ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga cttccaagaa
    84061 ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat gggtgctgaa
    84121 gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc tgttggtgac
    84181 gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt aatcatcgaa
    84241 gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat ggatgctgct
    84301 tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgattc aggcgaaggc
    84361 gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa atatccaatc
    84421 atctcaatcg aagacggatt agacgaaaac gactgggacg gattcaagaa attaactgac
    84481 gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa cactcaaaaa
    84541 ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt gaaccaaatc
    84601 ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg ctacacagca
    84661 gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat cgctgttgca
    84721 acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat tgctaaatac
    84781 aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa aggtttgaaa
    84841 tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg atcgactatt
    84901 gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt gctgaaatat
    84961 cagcaggtag agagtttttt ttgcaactag cttctgtata tgatgtttac aaagaatggt
    85021 acaagaaaag atcatccttg aaaaaggaaa gctaacaata actagcatca tataagagct
    85081 tttaaggata gaacagtaga ataggaatct atgagcaagt cctctcgtat aaataaaata
    85141 ggagggaagt agcagcatat ttttcataaa gtggctctct ttcttcattt ttactttggt
    85201 tgctcctatt tgaaaattcg agcacactca aatacaataa aaagaaatgg caagggagag
    85261 gattagatga aaaaagaaga aaaaatcgcg attttacaag agatcatccg aattaaatca
    85321 gtgaatggaa acgaaggtga agtagcagcc tatttgaata agcttttggc aaagcatgac
    85381 ataacaggag agattgtttc ttacagagat ggtagagata atttgattgc tcgttaccaa
    85441 aagggccaat caggaaaagt gttaggccta tctgggcata tggatgtcgt ggcggctggt
    85501 gatgaatcgt cttggaccta tgcacccttt gcagcagaaa tccatgggaa ccgtttgtac
    85561 ggacgtggtg ctacagatat gaagagtggc ttggcagcaa tggtgattgc catgatcgaa
    85621 ctgaaagagt caggaaaacc atttaacgga acagtcaaat tattagccac tgtcggagaa
    85681 gaagtgggtg aactaggtgg ggaacagctc acgaaagcag gctatgtaga tgacttagat
    85741 gccttgatca tcggagagcc aactaattac agtttgatgt atacgcacat gggatcaatc
    85801 aactatacag tcacctcgca cggaaaagaa gcgcatagct ctatgccaga ccaaggttat
    85861 aatgcaatca atcacctaaa tgaattcatt acgaaagcaa atgctgaaat gaatcacctc
    85921 gctgaaacaa tcgagaatcc tgtcctagga aaaacaatcc ataatgttac attgatcagt
    85981 ggcgggaatc aagtcaacag catcccaagc catgcgcagc tccaagggaa cattcgatcg
    86041 attccagaat acccaaatga taaaatcatt gcattgctgc aatcaatcgt caatgaattg
    86101 aatcaagaga cagactatca tttggaattg atgatcgatt acaacaaaat acctgtcaaa
    86161 gctgatccag attctccttt gattcattgc attcagcaac aattcagcca gccattacct
    86221 ttagttggag cggcagcaac aacggatgca gccgaattta cgaaagctaa ccattccttc
    86281 gactttgtcg tctttgggcc tggtgtcgtt actttgcctc atcaagtaga tgagtatgtt
    86341 gaaatcgata attatttgga tatgattgaa aaatatcaag gaattatttt atcctactta
    86401 gcgtaagatt tttttaataa
//
LOCUS       DACEJR010000004        82428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEJR010000004 DACEJR010000000
VERSION     DACEJR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.004"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..192)
                     /locus_tag="IW651_000264"
     CDS             complement(<1..192)
                     /locus_tag="IW651_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813026.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFI"
     gene            complement(489..2870)
                     /locus_tag="IW651_000265"
     CDS             complement(489..2870)
                     /locus_tag="IW651_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0813027.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2860..3507)
                     /gene="recU"
                     /locus_tag="IW651_000266"
     CDS             complement(2860..3507)
                     /gene="recU"
                     /locus_tag="IW651_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0813028.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3571..4110
                     /locus_tag="IW651_000267"
     CDS             3571..4110
                     /locus_tag="IW651_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0813029.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4200..4619
                     /gene="gpsB"
                     /locus_tag="IW651_000268"
     CDS             4200..4619
                     /gene="gpsB"
                     /locus_tag="IW651_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0813030.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4641..5008
                     /gene="rnpB"
                     /locus_tag="IW651_000269"
     ncRNA           4641..5008
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW651_000269"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5217..6383
                     /locus_tag="IW651_000270"
     CDS             5217..6383
                     /locus_tag="IW651_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0813031.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6429..7925
                     /locus_tag="IW651_000271"
     CDS             6429..7925
                     /locus_tag="IW651_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0813032.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8006..8689
                     /locus_tag="IW651_000272"
     CDS             8006..8689
                     /locus_tag="IW651_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0813033.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9334..10524
                     /locus_tag="IW651_000273"
     CDS             9334..10524
                     /locus_tag="IW651_000273"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0813034.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10626..11579
                     /locus_tag="IW651_000274"
     CDS             10626..11579
                     /locus_tag="IW651_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0813035.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11648..12997
                     /locus_tag="IW651_000275"
     CDS             11648..12997
                     /locus_tag="IW651_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0813036.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13180..13503)
                     /locus_tag="IW651_000276"
     CDS             complement(13180..13503)
                     /locus_tag="IW651_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813037.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13632..14795
                     /locus_tag="IW651_000277"
     CDS             13632..14795
                     /locus_tag="IW651_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813038.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14865..16214
                     /locus_tag="IW651_000278"
     CDS             14865..16214
                     /locus_tag="IW651_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0813039.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16259..16690)
                     /locus_tag="IW651_000279"
     CDS             complement(16259..16690)
                     /locus_tag="IW651_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813040.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17078..19720
                     /gene="alaS"
                     /locus_tag="IW651_000280"
     CDS             17078..19720
                     /gene="alaS"
                     /locus_tag="IW651_000280"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0813041.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19920..20516
                     /locus_tag="IW651_000281"
     CDS             19920..20516
                     /locus_tag="IW651_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0813042.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20638..21786
                     /locus_tag="IW651_000282"
     CDS             20638..21786
                     /locus_tag="IW651_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0813043.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21824..22237)
                     /locus_tag="IW651_000283"
     CDS             complement(21824..22237)
                     /locus_tag="IW651_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0813044.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22316..22747
                     /locus_tag="IW651_000284"
     CDS             22316..22747
                     /locus_tag="IW651_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0813045.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22846..23079)
                     /locus_tag="IW651_000285"
     CDS             complement(22846..23079)
                     /locus_tag="IW651_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0813046.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23262..24932
                     /locus_tag="IW651_000286"
     CDS             23262..24932
                     /locus_tag="IW651_000286"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0813047.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25079..25465
                     /locus_tag="IW651_000287"
     CDS             25079..25465
                     /locus_tag="IW651_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813048.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25452..26150
                     /locus_tag="IW651_000288"
     CDS             25452..26150
                     /locus_tag="IW651_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0813049.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26155..26619
                     /locus_tag="IW651_000289"
     CDS             26155..26619
                     /locus_tag="IW651_000289"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0813050.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26616..27527
                     /locus_tag="IW651_000290"
     CDS             26616..27527
                     /locus_tag="IW651_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0813051.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27762..28301
                     /gene="pyrR"
                     /locus_tag="IW651_000291"
     CDS             27762..28301
                     /gene="pyrR"
                     /locus_tag="IW651_000291"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0813052.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28315..29598
                     /locus_tag="IW651_000292"
     CDS             28315..29598
                     /locus_tag="IW651_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0813053.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29611..30537
                     /locus_tag="IW651_000293"
     CDS             29611..30537
                     /locus_tag="IW651_000293"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0813054.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30556..31836
                     /locus_tag="IW651_000294"
     CDS             30556..31836
                     /locus_tag="IW651_000294"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0813055.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31841..32917
                     /gene="carA"
                     /locus_tag="IW651_000295"
     CDS             31841..32917
                     /gene="carA"
                     /locus_tag="IW651_000295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0813056.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32922..36107
                     /gene="carB"
                     /locus_tag="IW651_000296"
     CDS             32922..36107
                     /gene="carB"
                     /locus_tag="IW651_000296"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0813057.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36161..36937
                     /locus_tag="IW651_000297"
     CDS             36161..36937
                     /locus_tag="IW651_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0813058.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36934..37866
                     /locus_tag="IW651_000298"
     CDS             36934..37866
                     /locus_tag="IW651_000298"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0813059.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37863..38579
                     /gene="pyrF"
                     /locus_tag="IW651_000299"
     CDS             37863..38579
                     /gene="pyrF"
                     /locus_tag="IW651_000299"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0813060.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38586..39215
                     /locus_tag="IW651_000300"
     CDS             38586..39215
                     /locus_tag="IW651_000300"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0813061.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39203..39517
                     /locus_tag="IW651_000301"
     CDS             39203..39517
                     /locus_tag="IW651_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813062.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39555..40196
                     /locus_tag="IW651_000302"
     CDS             39555..40196
                     /locus_tag="IW651_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0813063.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40227..40931
                     /locus_tag="IW651_000303"
     CDS             40227..40931
                     /locus_tag="IW651_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0813064.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40928..41830
                     /locus_tag="IW651_000304"
     CDS             40928..41830
                     /locus_tag="IW651_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0813065.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41988..43694)
                     /locus_tag="IW651_000305"
     CDS             complement(41988..43694)
                     /locus_tag="IW651_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0813066.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43884..44507
                     /locus_tag="IW651_000306"
     CDS             43884..44507
                     /locus_tag="IW651_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813067.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            44957..46006
                     /locus_tag="IW651_000307"
     CDS             44957..46006
                     /locus_tag="IW651_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813068.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            46003..46884
                     /locus_tag="IW651_000308"
     CDS             46003..46884
                     /locus_tag="IW651_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813069.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            46887..47657
                     /locus_tag="IW651_000309"
     CDS             46887..47657
                     /locus_tag="IW651_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0813070.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            47936..48790
                     /gene="aroE"
                     /locus_tag="IW651_000310"
     CDS             47936..48790
                     /gene="aroE"
                     /locus_tag="IW651_000310"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0813071.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            48968..50131
                     /locus_tag="IW651_000311"
     CDS             48968..50131
                     /locus_tag="IW651_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0813072.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50248..50658
                     /locus_tag="IW651_000312"
     CDS             50248..50658
                     /locus_tag="IW651_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0813073.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            50832..53060
                     /gene="topB"
                     /locus_tag="IW651_000313"
     CDS             50832..53060
                     /gene="topB"
                     /locus_tag="IW651_000313"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0813074.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            53198..53515
                     /locus_tag="IW651_000314"
     CDS             53198..53515
                     /locus_tag="IW651_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813075.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            53675..53911
                     /locus_tag="IW651_000315"
     CDS             53675..53911
                     /locus_tag="IW651_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0813076.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            53908..54306
                     /locus_tag="IW651_000316"
     CDS             53908..54306
                     /locus_tag="IW651_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0813077.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(54973..55917)
                     /locus_tag="IW651_000317"
     CDS             complement(54973..55917)
                     /locus_tag="IW651_000317"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0813078.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(56213..57157)
                     /locus_tag="IW651_000318"
     CDS             complement(56213..57157)
                     /locus_tag="IW651_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813079.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            57378..58592
                     /locus_tag="IW651_000319"
     CDS             57378..58592
                     /locus_tag="IW651_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0813080.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            58614..60629
                     /locus_tag="IW651_000320"
     CDS             58614..60629
                     /locus_tag="IW651_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0813081.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(60712..62085)
                     /locus_tag="IW651_000321"
     CDS             complement(60712..62085)
                     /locus_tag="IW651_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0813082.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(62111..62395)
                     /locus_tag="IW651_000322"
     CDS             complement(62111..62395)
                     /locus_tag="IW651_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0813083.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(62397..64412)
                     /locus_tag="IW651_000323"
     CDS             complement(62397..64412)
                     /locus_tag="IW651_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0813084.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(64591..64896)
                     /locus_tag="IW651_000324"
     CDS             complement(64591..64896)
                     /locus_tag="IW651_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0813085.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(64985..66016)
                     /locus_tag="IW651_000325"
     CDS             complement(64985..66016)
                     /locus_tag="IW651_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0813086.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            66223..67100
                     /locus_tag="IW651_000326"
                     /pseudo
     CDS             66223..67100
                     /locus_tag="IW651_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            complement(67252..68094)
                     /locus_tag="IW651_000327"
     CDS             complement(67252..68094)
                     /locus_tag="IW651_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0813087.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(68186..70063)
                     /locus_tag="IW651_000328"
     CDS             complement(68186..70063)
                     /locus_tag="IW651_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0813088.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(70060..71481)
                     /locus_tag="IW651_000329"
     CDS             complement(70060..71481)
                     /locus_tag="IW651_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0813089.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(71484..74873)
                     /locus_tag="IW651_000330"
     CDS             complement(71484..74873)
                     /locus_tag="IW651_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0813090.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            75130..76521
                     /locus_tag="IW651_000331"
     CDS             75130..76521
                     /locus_tag="IW651_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0813091.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(76594..77241)
                     /locus_tag="IW651_000332"
     CDS             complement(76594..77241)
                     /locus_tag="IW651_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0813092.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(77249..77935)
                     /locus_tag="IW651_000333"
     CDS             complement(77249..77935)
                     /locus_tag="IW651_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813093.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(77941..79014)
                     /locus_tag="IW651_000334"
     CDS             complement(77941..79014)
                     /locus_tag="IW651_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0813094.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            79236..79910
                     /locus_tag="IW651_000335"
     CDS             79236..79910
                     /locus_tag="IW651_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0813095.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            79915..81255
                     /locus_tag="IW651_000336"
     CDS             79915..81255
                     /locus_tag="IW651_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0813096.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(81404..82390)
                     /locus_tag="IW651_000337"
     CDS             complement(81404..82390)
                     /locus_tag="IW651_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0813097.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        25944 a        14756 c        16819 g        24909 t
ORIGIN      
        1 aataaatgca aggataggag caattaaagt aaatttattt aataataaac aaaatttttc
       61 agctttttta ttcttggaat aacgaggtat tgttttcgat aaaaaaagat ggtgcagaca
      121 aattatgata attaagcata ctatcagata actcaaaatg aagccttctt tcaaaaaatt
      181 attcaaaacc atatattgta ttttttattt gataataaca atgggattat taagaagtgg
      241 cactaaataa aagactagtg tgtatttagc cgatttaatt ataaacaagt aaatggataa
      301 agtatatgta atgataaaag aaaaatggat tttctaattt ttaagctaaa tgttagatca
      361 cacatgtata tgctggatag tcttataaaa aatcatgtaa tacagcttgg tacatgattt
      421 ttatttgtag ctgtttttta catagacaca aaaaagaaac cacgcttgta gacgtggttt
      481 cttttttact atctgtttcc agaagctgag gtgctgctgg atggtgtatg gtcatttgca
      541 gcatccgctc cgctagttgc tggctcactg gaagaagcag gaggagcaga tgaagacggt
      601 gtcgagcttt cttcggatga agaagaagga accgttgttg aagacgctgg ctcgcttgtt
      661 tctgctgtcg tgctttcttt tgaggactca gcagttgatt cgctttgact agtagagcta
      721 gagctttgag tcgttgtctc tgttgttgag cttccaggag tctggacata gctggaagat
      781 ggaatagttg ttgatggtgt aattgcattc gatctagctg tatattgatc tttgtagtac
      841 agttcaccgc ccacacgaat taatccgctt ggcatctccc agtctgtatt tgtcacgtta
      901 gctgaaacat actgcataag ctcgcgataa acatcagagg ctacgtgtga cgattctgaa
      961 gtgacaggtg tcattttttt attgtaacct gtccacacgg aaatcgaata atttggcgta
     1021 tagcccgcaa ataggatatc tggatacacc ccagaagaaa taccaagttt tgcatactcg
     1081 tcatctgtgt aattggatgt tccggtctta ccggcttgat ataacccagc gatttgcgca
     1141 ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt ctgtgatcat ataagctgtt
     1201 tctggagaca tggctgtttt tccatctggc tcataagttt cttctgttcc gtcttggaag
     1261 acgattttat taacatattg aggtttgtaa taagtccctc cattggcgaa agccgcatag
     1321 gctgctgcca tttttaagga agaagctccg tattttgtcc catcttgttc ttccgtatta
     1381 ctagagatcg cattcgattg atggatcgta ctgtattcga tcccaagatt ttttaagaaa
     1441 gaagccactt tatctgaacc gacttcattg aataatttga ctgctggaac attacgggat
     1501 aagtaaagtg cttggcgtaa agtaatggtg cccatatatt gattatccca gtttcctaca
     1561 ggagtcgaag tcccttcata gttatacggt gcgtcagtaa tagtttttcc agtagaatat
     1621 ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt tcatcgtaga accaaaatca
     1681 cgggaagtat tcactgctaa gttgttgcct aaagttacgt cttctgcgat gtgacgtccg
     1741 ccaatctggg cttttacttt cccggtattg gtgtcgataa gggtggaggc aacttgcatc
     1801 tcgtcgtctg ggtaagagac atactgatcc gtgttgacga tatcatatag tttcttttgg
     1861 gcgtccagat ccaaattcgt ataaatctct aaaccgtctg tgtagacatt cttatctgtt
     1921 ttttcttgta cttcattgat gacttctttg acataattat cgacgatctt agtattgtca
     1981 tcggattgag ttagttcttg cagaccatcc gttaccggta cattaacagc ttgatcatat
     2041 tctgtttgag agattttttc gttttgaagc atcgtataca aaacagtatc tcgtcttttc
     2101 ttcgcctgat ctggataaac ataaggatca taggcggaag gggcttgagg catcccagca
     2161 agcaatgctg tttgcggtaa agaaagttct gataatttct taccaaagta catctcagaa
     2221 gctgtctcca tcccgtacaa gccgtttgac atatacactt tatttacgta gtaggtcaag
     2281 atctcttgtt tcgatttttt ttgttccaat cttacagcca tccacgcttc ttgggccttg
     2341 cgtttcagag tctgatcctc ggcactggtg gagaagaagg aaagtttgat caattgctgt
     2401 gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt tagagagggc agaaccgatg
     2461 atccgaatag ggtcaacccc gatgtgttta taaaaacggc gatcttctac cgacacgatc
     2521 gcatcttcta atgttttcgg taattcattc gcagaaattt tttcacgctt ttctgcgcct
     2581 aaatcttcga atagttcgcc atcttgtgta tatagtttgg aagatacggt agcgtctaat
     2641 tttttatccg tcaattcggg cgcatctttt gcgtaatacc agaaaagtcc tacacctgct
     2701 aaaaagagga tacagaaaaa tgagagtatt cctaaaagga tcttgataaa taatcctttt
     2761 ttatgtgttc cagaggactt tccagaacct ttgccagaat tcttcttcac actcttttta
     2821 ggagtaggct gtttctgtcg tctagagctt ctagtttgtt catttgccat tcttgctttc
     2881 tccttcaata gataatgttg ataaatagaa gtcaaccgcg tccaaataag gaatccgcgg
     2941 agaaatacca gttttgatct ctattcctag ttcttcgatc aaagataaag gtagtgattt
     3001 gcgtcctttt tccttttggg cgtcccagta agcaatcaaa tgaacactat tcaaataaaa
     3061 acagcgcttc aaagacgaaa accataacaa gacaaagcag accccatttt gttcgaggca
     3121 tgctttcata tgttcgattt gatgggcatg gaagttttta aagggaaaag atgttttgtt
     3181 cttggtttct tttgcttcaa agtccaagta aaagcctcga tagacgccat tatagtctgt
     3241 agttgaggct tgggtaaagt aggcttcctt gatcacagca gcacttctct taggatagtc
     3301 tactttcacg atttggatag gggtaggctt tttatggata accgccaacc cgcgggaaag
     3361 ataatattta ttactttcat tgattgcttc ttcaaagcgc atcccccgat tgccgaattc
     3421 agtgatctga tttctttttt cttgaatttt ttcgacggga gaggcattct tataataagg
     3481 ttggccattt ggatagcgca ataccatctt taatcacact cctaggccac attatagcaa
     3541 aaaagcggat agaaagaaaa ggaatctgag atggataagt caaaagtaat ctatgtgtct
     3601 gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg atcctaaaat ccaagttgtt
     3661 aaaaatgttt taaaaaaaga actccatcag ttagcagaag aagggctgga atgggtactt
     3721 gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa tagtagcgga attgaaagaa
     3781 gaatatccgg aactatctct tggtattttg tatccttttt ctgattttgg taataactgg
     3841 aatgaaggga accaagaaaa aaaacaatta gcagaacaat tagcagatta tgtcgaagct
     3901 gttagccatc aaccctacca atcacctagt cagctaaaga atcacactcg ttttatactt
     3961 gagcatacag tgggctgtct gctgatctat gatgaagaat tccctggaaa aacgaagtat
     4021 ttttttgaag atgcacagaa ttaccaagca gatcatccct acgaaattcg gctgatcagt
     4081 atggacgatc ttcaaaattt tagtgaataa tcgatattgt gtttgattta tcaacttttt
     4141 tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat gaaaactaga ggtgtaaata
     4201 tggcgaattt ggtttatagt cctaaagaca ttttacaaca agaattcaaa acaaaaatgc
     4261 gcggctatga tcccgttgaa gttgatgaat tcttagacaa tatcatcaaa gattatgaaa
     4321 cttacagcaa agaactcctt gctttacaag aagaaaacga tcgattgagt gcaaaagtag
     4381 ctcaattatc taaaactcaa ggagcagctc aaacacgcgt gcaacaaaca gaggcaccaa
     4441 agagcgcagc agtgaccaat tttgatatct taaaacgctt gtcaaatctt gaacgtgaag
     4501 tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa accagcacaa cctaatccaa
     4561 ataactacac aaatgcagac acaagcttag atgataatga aaaaacacgt caattttaat
     4621 ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc tgacttgatc aggctgagga
     4681 aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg tgcttagcga aaccataagc
     4741 taaggtactc tttggagtaa cggcaggaaa acaagctaag gcttcggcta tgcttaagta
     4801 tccttgaaag tgccacagtg acgaagcggc tggggaaacc catccggtgg aacgcggtaa
     4861 acccctcgag cgagcaaccc aaacaatggt aggggcgctt tttgtctgga agaagaacag
     4921 gacaaaaagg cagtataact gcagatagat ggttaccgct ttgttttttc tccctggaaa
     4981 agcaaagtac agaacatggc ttatagaaaa ccgcaaacat cagggaatta ggtcgtgaca
     5041 aaagtaatgc gttataagaa acaagaaaaa catttgaaag ctatttttca atacttcaga
     5101 tgaatcgttc ttattacaaa acaaactttt gtatcaccgg ttattcggaa tttgagtaaa
     5161 agtgtggggt aggactgatg tcccacctcc tttttttata aataaggaga ttgtgtatgg
     5221 aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag cgggctagaa gcactggttg
     5281 gtaaagaatt aagagatctt ggaatcgaat gccaagttga gaacggacgt gcacgttttg
     5341 aaggaacaat ggaaacgatc gctacagcta atttatggtt acgtacagca gatcgagtaa
     5401 aaatcgtggt tggggaattc gatgcgtata cgtttgatga attgtttgaa aacgtaaaag
     5461 cattgccttg ggaagatttt cttccactag atgcagcttt tcctgtagct ggaaaatcga
     5521 tcaagtcgaa actatatagc acacctgatt gccaagcgat cacaaaaaaa gcaatcgttg
     5581 aacgtctccg tacctattat caccgtccag cttctgtacc actgacggaa actggcgcac
     5641 attttcagtt ggaagtagct ttgctgaaag accatgtgat ggtaactctg gatacgacag
     5701 gaccaagttt attcaaacgt ggttatcgct tagaaaaagg tggagcacca ttgaaagaaa
     5761 acatggcagc agcacttatt atgttgacaa attggcgaaa agaccgtcca ttttatgatc
     5821 ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt aataggccat aatatcgcac
     5881 caggattcaa tcgtgagttt gcttgcgaat cttgggattg gttcagtcaa gaaatcatgg
     5941 aaaatgttcg atcagcagcc gaagaaaaag cagattatga tattgaatta gatattactg
     6001 gttcagatat caatggacgg atgattgaaa ttgccaaagc caatgcagaa gaaatcggat
     6061 taggctcttc gattaccttt aagcaacaag cagtcaaaga cttcaaaacg gataaagaat
     6121 acggggtcat cgtggctaat ccgccttacg gagaacgtct aggagaagaa gaaactgttc
     6181 ggaaattata taaagaaatg ggcgatgtat tccgtccatt aaaaacatgg agtaaatata
     6241 tcttgactag cgacttagcg tttgaggaat tttacggaca aaaagcaacc aaaaaaagaa
     6301 aattgtacaa tggtgcattg cgtacggatc tattccagta ttggggaact cgtccgccta
     6361 gaaaaaaaga gaaagacgaa taatcttact aattgattca ttcaattgga tgtttggagg
     6421 aatagggaat gaaagaaaaa gagtttttac aagaagtaaa agaaatcaat cttctacaac
     6481 aagctttagg aatcctcgat tgggatacac aaacaggtat gcctgaaaaa gctagtgcat
     6541 accgaagtga agtagacagc tatctttaca gcatttattt ttcaaaaaaa atcggtccaa
     6601 aaatcaaaga agccattcgc tatttttctg aacatccgga agaattgtcg gaagttggaa
     6661 ctgccgtttt tgccaaagta aaagaagagt atgatttgga acatcgcgta ccggaagatc
     6721 ttatgagaaa atatacagct gccgtttctt cagcacacgg acaatggcaa aaagctagag
     6781 aaacacaaca attttctgat tttcaacacg cattgaatca aaatattgaa ttaacaaaac
     6841 agttgatacc ttactggaga aaagatgaaa agacgaatta tgatgttttg ctgaatcaat
     6901 acgaacccgg gatgactgtc gaaatccttg ataaggtatt tgatcagctt aaagaaggaa
     6961 ttctttcgat ccgccgtacg ctccaagaaa aagggactga acctgaaaca gactttttaa
     7021 atcggagaat gacgaaaaaa caacagaaga gatttgtagt gaaagtaatc gaacaacttg
     7081 gttatgattt tagtcgtggt cgtttagatg acactgtcca cccatttatg actggaatta
     7141 atccgaatga tgcacgaatc acgacacgat ggaacgaaac agatttcaaa atggctgttt
     7201 ttggagtgat ccacgaggct ggtcatggta tgtatgaaca agacatcgat gataaatatg
     7261 cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca tgaatcacaa tcgttgttta
     7321 acgagatcat tattggaagt agtcgcagct tctggaaaaa gcaatatcct ttctttcaag
     7381 aatgtgcaga aggaacattc gatgatattc cttttgacaa attttatcgt tcattgaacc
     7441 atacaaaatc aagtttggtt cgtatcgagg cagacagttt gacttaccct ctccatatca
     7501 tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg ggtagatgtt gctgaactac
     7561 ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat acgaccaaca aatgatgtgg
     7621 aaggcatcct acaagacgtt cattggtcag gtgcaagctt tggttatttt ccctcttatg
     7681 cattagggta catgtatgcg gcacaattac ttcattcttt gcagcaagaa atagatgtgg
     7741 aaaatgttct agcgtcagac gattattctc caatcaaaaa ctggatgtca gagcatatcc
     7801 atcaatatgg tgcatctaga aaaccaaatc aattaatcat ggatgcaaca catgaaccgc
     7861 ttaatccgca atacctgatc gactatatga aagacctgta ttttaatgta tatcaggtaa
     7921 aataaaatac aatttacaaa gatagctgaa attcatctaa tctatagatg ttttcagcta
     7981 tttacttaag aaagaagtgg aacgaatgga taaattacaa gtgatcgctg attatagtat
     8041 ggaccaatta gcgcatgatc aaacaggaca tgggagcgat cataccaaaa gagttgtcaa
     8101 gttagctgaa aaaattttgg agacggaacc gcaggcagac cgctttgtga cgcttgcagc
     8161 agcttaccta catgatacga ttgatgataa agtcgttaaa aatgagaacg aggcaaagca
     8221 acagcttcgt gtattccttc gctctttgcc aatagtagag gaacagatct cgatgatttt
     8281 ttccattatc gaaaatatgt ccttttctaa aaatctctct gaagcggccg aactatctct
     8341 ggaaggtaag atcgttcaag acgctgatcg tatcgaagca ttaggagcaa tcggcatttt
     8401 gcgtactgct tatttcggcg gtggacatgg gcacccgatt tttgatcctg aactgtatcc
     8461 gcaaacattc aaggacaaag aaaattaccg aaaaggcacg acagtgatca atcattttta
     8521 tgaaaaactg ttcttgctgt ctgataaaat gaatactgat tacggatatg aagaagccaa
     8581 acgaagagaa accttcatgc gtgaattctt agaagaattt tttctggaat ggtctattga
     8641 cgatgaaaat tttactccag acaattggat caaaaataac aaaaaatgat ggaggttgtg
     8701 acagaagtgg tcagctccaa gatatttcgg atcattttcc gtaaaagtta cttctggaac
     8761 actgtttacc aagaggtcgt gagcctgccg ttcagctccg agtcacaagg aacaatttca
     8821 aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg attcggaatt ggcaggagaa
     8881 caccgtttat tcggaagaat aggactgtga caagactttt gtcacagtcc ttttttcgaa
     8941 ttcaacaagt aaaactttta agaaatattc agagagtaaa attaaaaatt gctgataaac
     9001 gaagatggaa attttatgta tttttattgg tctatgcaat aaataaatta aatattcaga
     9061 atacaattga ttttaacgaa agggcttcta tcaatggttg tgttgtattt atctgcccag
     9121 gtagagtaaa atagatcatt atctaaaagg taattgttca tactatataa gtcatttgaa
     9181 agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat tctcataatc agtgtaagcg
     9241 ttatcaaaaa tatagcatac ttgctttgta gatgaatgac acgatatatg tttggtatgt
     9301 tctgcaagac agatgaatat ggaggtggag acaatgtctt ccaaagaact aacaatgtat
     9361 ttagagaaag aattggatca actgaaagaa aaggggttat acaatacgat cgacatacta
     9421 gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa agatgatcaa tctagcatcc
     9481 aacaattatc ttggatttgc caatcgcgaa gaattgaaaa aagcatgtat tgaagcaacc
     9541 gaaacttatg gtgtaggagc aggagcagtc cgaacgatca atggttcgct gaaaatccat
     9601 cagcaattag aagaaaaaat cgcagaattc aaagggacag aagcagcaat cgcctttcaa
     9661 tctggattca actgcaatat gggcgctatc tctgcagtaa tgacaaaaga agatgctatc
     9721 ttatcggatg aattaaacca tgcatctatt attgacggtt gccgcttatc cggagcaaaa
     9781 atcatccgaa ttaaacatca agatatgaaa gaccttgaga aaaaagcaaa ggaagcgata
     9841 gaaagtaaga aatataaaaa aatcatgtat atcacagatg gtgttttctc catggatgga
     9901 gacattgcac gattgccgga aattattccg attgttgaaa aatatggact gatcacctac
     9961 gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg caggaaccgt caagcatttt
    10021 ggattaagcg acaaaatcga tatgcaaatg ggcacgttat cgaaagctat aggtgttgtt
    10081 gggggttatg tagctggatc gaaaacacta atcgactggt tgaaagctag aagtcggcca
    10141 ttcctatttt ctacttcgct aactccaggt gcagctgcag ctgcattggc ttcgattact
    10201 ttgatgcaag aacatccgga acttgtcgaa aaagtttggg aaaatgctaa ttattttaaa
    10261 gaagaattga aaaaagtagg ctataacatc ggtatgtctg aaacacctat tactccagtc
    10321 attttgggag acgaaaaagt gactcagact ttctctaaga agctgatcga acatgggatt
    10381 tatgctaaac caatcgttta tccaactgtg cctcttggga caggcaggat cagaaatatg
    10441 ccgactgcgg aacatacgag ggaaatgctt gatgaagcag tagcagttta tcaaaaaata
    10501 ggaaaagaaa tggaaattat ttaactaaat ttagtttttt cgtctattta aaattagaga
    10561 acatgttcac gcatatattg ttaatcatct gaagatagta gtaaaaaaga tcagggagga
    10621 tcatcatgaa aaaagtattg gtgacaggat gtttaggaca aattggttca gagttgatca
    10681 cacggctacg tcaagaacta ggcagtgaaa atgtcattgc gacagatata cgcatgcctg
    10741 aagtcattga gaaaaacgat atctttgaga tcctagatgt aatggattac gaaaaaatgc
    10801 gcagcatagc agaacaatat caagtggaca cattgattca tttggctgct cttttgtcag
    10861 ctgttgcaga agcaaaacca caattagcct ggaatttgaa cgtgactggg ctggtcaatg
    10921 cattggaagt cgctagagaa ttccagctga aattttttac gccaagttca atcggcgcgt
    10981 ttggtccaaa tactccaaaa gatcataccc cacaagatac tcttcagcgt cctacaacca
    11041 tgtatggggt aacgaaggta gctggagaac tactatgtga ttattattat gtgaaatatg
    11101 gcgtagatac tcgtggtgtc cgtttccctg gattgatttc ttatgcaacc ttgccaggag
    11161 gcggtacgac agactacgca gtcgatattt attattcagc aatcaaagaa ggaaagtaca
    11221 ctagtttcat tgatcaaggt acatcaatgg atatgatgta tatgccagat gcgatcgatg
    11281 ccattataaa actcatgaat gcagatcctg atcgattggt tcatcgaaat gccttcaata
    11341 tatcggcgat gtcttttgac ccggaagaga tcaaagcatc tattcaaaaa atcataccag
    11401 attttgaaat ggattatgat gtggatcctg tacgtcagtc aatagcagaa tcttggccaa
    11461 attcactaga tgtttcttgt gcgcaagagg aatggggctt ttcgccgaaa tacgacttag
    11521 atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa attggtagag acggtataaa
    11581 acaagaacca catccaaaaa gttttttata cttttgggat tggctatggt atgaaagggg
    11641 acaagggatg gaagaatatc aagccacgcc agtaaaagaa gtaaaaatag aaaatgaatt
    11701 aaaacgtacg atgggatttt ttactgcttt atcgactgtt atgggcacag tgatcggtgc
    11761 aggggtattt tttaaagccg caagcgtagc agaagttaca ggatccgcca gtttgcatat
    11821 gttttcttgg tttctcggtg gtatgatttc tgtctgtgca ggactaacag gagctgaact
    11881 ggcagctgct attccagaaa caggagggat gattaaatat atcgaacgga tttacggaaa
    11941 tacagcagct tttttattgg gctgggcgca agtcgtgatt tattttccag caaatgtagc
    12001 agccttgtcg atcattttcg gtacacagtt cgtcaatcta tttggtcttt cccagtcaat
    12061 gattgttccc gtagctgtta cagctgctgt atcgatcctg ttgatcaatt ttttaggttc
    12121 taaagctgga ggggcttttc agtcgattac gcttgtctgc aagcttattc ctttgtttgt
    12181 gatcgtcatt ttcggtttgt tccgacaaga aggagtagac ttccaactgt ttccgatcca
    12241 agcaggagaa aatctttcat ttttctcagc cttgggagcg ggcttgctag caacgatgtt
    12301 cgcatacgac ggttggatac acgtaggaaa tatttcggga gaattgaaaa agccggcgaa
    12361 agatttgcca aaagcaattt ctttaggaat catcggaatc atgatcgttt atttattagt
    12421 aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc gcaggaaaca gcaatgcggc
    12481 aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga agactggtca cagttggtat
    12541 cttgatttct gtttatggaa ccatcaatgg ctataccctt actggaatgc gtctccctta
    12601 tgttatggca aaagaaaaca acctgccttt tagcaagctc tttgctaaac ttcatgataa
    12661 aactaaagtt cctgtagccg caggtatctt agagctagtc atcgctattg gcatgatgat
    12721 gatcggtggt tttgatacac taacggatat gctgatcttt gtcatttgga ttttttatac
    12781 gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa cctgatttgt ttcgaccata
    12841 caaagttcca atgtatccgt ttattccact agtagcaatc attggaggga cttttatcgt
    12901 atcaagcacg cttatcactc agaccttttt agcttcgatg gggatagtat tgacacttgc
    12961 tggtatcccg atttatctct atttaaaacg tagatagcta aaacattcga attttattag
    13021 aactgcactt ctctgccaaa caaaatattt tatttaaatg aggagtacgt ggaagaaatg
    13081 tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt tgtctacgat gaaatagcaa
    13141 agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt tactttgatt tgcgtaactg
    13201 aactattcgg cgtacgccgc gataaatatt taataaaacc aagcaaccag aaatgaaaat
    13261 gggaagccaa atcagataga cagggtagcc aagcacttta gacaaagcat gtggagtcat
    13321 tttatccatt tcaagaagtg cttgctgcca agtctgaaga ttgaagacta gcatgaaacc
    13381 cgaaataatc aggagaataa atcctaaata atgaattgta gttctttttc ttacataagc
    13441 gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca aaaacaaagt acatattcag
    13501 catctcgaca ccccctttgt gtttatctta ctttactttg aaagaaatga gaaataatag
    13561 cctcgttact tgcttgaaac agcaaaggac tgctaagata agagagatac aaagaatcaa
    13621 ggaggagtca tatggcaagt tatatcgaac cgattcgttt tgcgctgatt gtttttccat
    13681 ttttagcctt agcgatctca tctgtttttt ttatttatga atatcgaaag tatggaacat
    13741 ttctagtaac aagggcagtt attttgtatt ctttcgtttt ttatttattg tgtgcgtatt
    13801 ttttggtgat cttaccactt ccggcaaagg agacggttgc ccaaatgaca gggccaaaga
    13861 tggagctgca tttatttgct tcatggcaac atttcagaga caacacggta ttggtcttgt
    13921 ctgaacctag tacctacctg ccggcaatga agcagagtgt atttttggaa ccagctttta
    13981 atgttttatt actcatacca tttggcattt atttaagata ttatttccgg ctttctttta
    14041 tgaaaacgat gattgctagt ttctgccttt ctctattttt tgaattgacg cagctaagtg
    14101 gactttattt tatctaccca agaccatacc gcttatttga tgtgaatgat ttagtgacaa
    14161 atacattggg tggaatcatt ggttttgtcg ttacgccgtt atttacattt atgcttccta
    14221 caagaagacg aatggatgaa ctttcttatg aaaaaggaaa gactgtctca ttgactcgac
    14281 gcttggtcgc ttatttgatc gactggacat ttttatctct tattaatttg gtagcggcga
    14341 ttttctttaa aattgcgttc gattcagggg cagttacttc tcatatctgg tggcttttat
    14401 tagaagtagg ggtatacttt atgattgtac cgtttataac caatggatgg acacttggca
    14461 aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga acgaatttct ttaaaagcat
    14521 tgatgatccg ctacgggtat ctgtactttc ttttttatgg tttaagctgg ttggggcagt
    14581 tgcttcagca aatcatgaat tcgacaacga atacggtgtt gcagatgagt gcagtattca
    14641 tttctctcgg tgtcttgtta ctacaaggta tttttatttt aaatatcttg ctatcatttt
    14701 tgagaaaaaa acggattctg ttttatgaaa aagctagtca cacatatact gttagcacga
    14761 ttggccaaac aaaggatgat gattctcgaa attagctgag caaaaacgct ttgtctgtgt
    14821 tataatgcca aagtcgtgat aatttaaaga gaagaggtgc acgaatgaat aattttgata
    14881 tattccgctt caaaccgttt atcaatgaag cgttaaccgc aaagggcttc aaacagccca
    14941 cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg aaaaaatatc attgggcaat
    15001 ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc tttgtttgaa aaaatcaatg
    15061 cagaaaaaga ggaagtacaa gttgttatta cagctccaag tagggaacta gctgcacaga
    15121 tctatgaagc agctacacaa atcgctaaat tttctacacc ggagatccgt gtctcgaatt
    15181 ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct gcatcaccaa cagccacagg
    15241 ttgtagtagg cacgcctggt agaatattag acatgatgaa tgaacaagcg ctgaagattc
    15301 acaccgcatt tgcatttgtt gtcgatgaag cggatatgac attggatatg ggttttttaa
    15361 atgaagtaga ccaaattgca agtcgattac cagaaaagct gcagttttta gttttttctg
    15421 ccactattcc agaaaaactt cgtccattct tacgaaaata catggaaaat ccaatcattg
    15481 aagaaatcaa accaaaagca gttatttctg aagacattga aaattggctg atttcaacaa
    15541 aaggtaaaga tgagaatcaa atgatttatc aactgctgac aattggtcat ccttatttag
    15601 caatcgtatt tgccaataca aaacagcatg tggatgaaat tgccgattac ttgaaaaacc
    15661 aaggcttgaa agtagcaaag attcatggag acatcacccc aagagagaga aaacgagtga
    15721 tgcgacaagt acagaatctg gagtatcaat atgttgtagc gactgattta gctgctagag
    15781 ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga aattccagaa gatctagact
    15841 tctttatcca tcgtgtcgga cgtactggac gtaatggctt aaaaggaacg gccattacgt
    15901 tatattcgcc aaatgatgag caagccattg atgcgataga aaaattgggc gtctcatttg
    15961 aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata cgatcgtaat agacgtgcca
    16021 aacgagaaaa atcaaaagat gtattagatc ccacgctgat tggtcttgtg aagaagaaaa
    16081 agaaaaagat caagcctggt tataagaaaa aaatcgattg ggcaattgca gaaaaaaata
    16141 aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac tgctcgtaaa aataagaaaa
    16201 attcacataa ataaaaaaca agaggttgtg acaaaaggcg tgtcacaatc tcttgttttt
    16261 aagctagact ttttttcatt ccgatacaaa gtacttcatc ttcaataaaa ggttctgtgc
    16321 aagtatgata cccttgtttt tcataaaaat ctacagccgt catttcagct actacataac
    16381 tagtctgaca accttctgaa accgcccgtt tttcagcagc ttcaagcaac tgaccgccta
    16441 acccttttcc tcggtattct tttgctacgc aaaatcgttc cggcttgaag caagtaggtg
    16501 ttttcttttc ataacgcaaa gtagctactg gtcttttttg atcaagaaat agataatagg
    16561 tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc aggaatccct tgttctaata
    16621 caaaaacttg atatctcaaa aagcagctag ctgcgcgtat ccattcatcc gctccaacta
    16681 tttttttcat catattcctc cttgtcatga atagcagaga tgtattgatt cctttattct
    16741 tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg aaaataaaaa ttgagatatt
    16801 gacagtgatc aatagaatgc gtatactaaa taagtacgaa aaggatatga tttgtttttt
    16861 aagactcaca gaaaattctt tcttggctga aaaaagaata gtcactaatc aaattgctcc
    16921 ctttttctat tttggtagaa ataccgacgc agctcagcgt tatcgagttt aagaggtaat
    16981 gattacacat cattgcaatc aaggtggtac cgcgagtttt cgtccttggc tgtgatggtg
    17041 tttttttgta tttaatttat agaaagcagg taatgacatg aaacaattaa gtagtgcaca
    17101 agttcgacaa atgtttcttg atttcttcca atctaaagga catacgattg aacctagtgc
    17161 gtctttgatc cccgtgaacg atccaacctt attgtggatc aactctggtg ttgcaacatt
    17221 gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca agaatcacaa atgcccaaaa
    17281 atctattcga acaaatgata tcgaaaatgt cggtaaaaca gcacggcacc atacgatgtt
    17341 tgagatgctg ggtaattttt cgattggtga ctatttcaaa aatgaagcca tccactgggc
    17401 atgggaattt ttgactgatc caaaatggat ggcattcgat ccagaaaaac tttatgtaac
    17461 agtttatcca aaagatacag aagcaaaacg catctggcat gaagaagttg gcctgccact
    17521 agatcacatt attgatgtag aagacaactt ctgggatatc ggtgcaggcc catgtggtcc
    17581 ggatacggaa attttctatg accgtggaga agcttataac gatttagcag aagacgatcc
    17641 tgaaaactat ccaggaggag aaaatgaacg ttatttagaa atctggaact tagtattttc
    17701 agaattcaac cataagcctg ataacacatt tgaaccactt cctcataaaa acattgatac
    17761 ggggatggga ttggaacgca tggtctcaat cgttcaagat gcgtcaacga actttgagac
    17821 agacttgttt atgcctatta tccacgccgt agaaaagcta agcaaacaag taaaatatgg
    17881 tgaaaaccca gtaacagata tttcttacaa agtaatcgca gatcatattc gcgccctttc
    17941 atttgctatt ggcgacggtg cattgccatc gaatgaaggg agagggtatg tcttacgccg
    18001 tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt attgaacaag cattcttata
    18061 tcgtttagtc cctgttgttg gggaaatcat ggtaagttat tatcctgaag tacttcaaca
    18121 aaaagaattt attgaaaaag ttatccgtac agaagaagaa cgtttcaatg aaacaatcaa
    18181 tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta aaaaacgaaa acgggacgac
    18241 tctagcagga aaagacatct tcaaactata cgacacatat ggcttcccgg tagaattaac
    18301 ggaagaggtg gcagaagatg aaggactgaa agttgatcat gctggttttg aaaaagaaat
    18361 ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact gaaaaatcaa tgggggtaca
    18421 atctgcttta ttgacagata tcaaagtaga aagtaaattc attggataca cagaacaaga
    18481 atcagatagt caattattgg tcatcgttaa aggtgagtct cttttgaatg aagtcgcaga
    18541 aggtgaagct caaatgatct ttgatcaaac acctttctat gcagaaatgg gtgggcaagt
    18601 agcggatcaa ggatggatca tgaatgaagt aggcgaaatc gtagcagttg ttacagatgt
    18661 tcaaaaagcg cctaatggtc aatttatgca caccgtagaa gtaaaagcac caatgagtga
    18721 aggacaaacc tatcatctat tgatcgatca atcgatgcgc aatcggatca tcaaaaacca
    18781 tacagcaacc catctactgc acaaggcatt gaaagaagta cttggcgagc atgcaaatca
    18841 agctggttct ttagtcgctc ctggacattt acgattcgac tttacccact ttggtcaaat
    18901 cacttcagat gaattagctg aaatggaaag aatcgtcaac gaaaaaatct gggaagctat
    18961 cccagttgaa acaattgaaa cagatatcga tacagccaaa aacatgggtg caatggcttt
    19021 gtttggtgaa aaatacggac atgacgttcg agtagtaaat attggcggct ggtcaatcga
    19081 actgtgcgga ggcacacatg tagcaaatac agaaaacatc ggaatcttca aaatcgtttc
    19141 tgaatcagga attggtgcag gcgtgcgacg aatcgaggct gtaacaagta aagaagcgta
    19201 cgaactactt gaagaagaag aaaaacaact aaaacaagta gcaacaattg ttaaatcacc
    19261 tcaattgaaa gaagttgttt caaaagcgga acaattgcaa cagcaagtaa gagagctaca
    19321 aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa caagctggtg atgtattcaa
    19381 agatccagaa acgatcaatg gaaaaacaat cattgcagca caagtaaaag tgaaagatat
    19441 gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa aatatttcag atattcttgt
    19501 tttagcaaac gaacaagaag gaaaagtcag tctgctagcg gcaatgacga aagaatcaaa
    19561 tgaagctgga ctaaaagctg gcgacttgat taaagcaatc gcaccgaaag taggcggtgg
    19621 cggtggtgga cgtccagaca tggcacaagc tggcgggaaa aatcctgctg gtattccaga
    19681 tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag ggcgtttacg tttgacagaa
    19741 aaacaaaagt tcaaacattg aacgaaacat gattatgggg gcgaagacaa acaagtcttg
    19801 ccctcatact tttttcaatt ggatggcgca tctaatcaaa tggtcggatt cggatggtct
    19861 ttagttgacg aaagtgacag aaatttttac gattagtata aataaaaagg ggtgaaaaaa
    19921 tgaatagtag aaataagacc tttcgactag tcttacgtgc gatcttgtta gcaatcatca
    19981 tcgtacaagc gatggttcct tggctggggt ttattccatt aggattcatt agtttaacaa
    20041 ttatccatat cacagtaatt attgctgcag tagtattagg cccaaaagat ggcatggtga
    20101 ttggcttgtt ttggggaatc gctacaattg ttcgtgccta tgccatgcca acaacgccat
    20161 ttgatacatt agtgttcacg aatccgatta tctcggtagt tcccagagta cttgttggct
    20221 tagtagcagg cttggttttc cactggattt atcaaaaaaa taaatcaata acgatcagct
    20281 cagcctttgc aggggtactg ggttcattgg tcaatacagt cttagtgtta ggattcatgg
    20341 gattatttta tacaggtgca acggcagatg cctatggtgt agactctgct cttttattta
    20401 aaaccttagc aggggttgca gcaatcaatg ggataccaga agcaataggt gcgggaatca
    20461 taacaccatt actagcaaaa gcgttatttg cagcaacacc gttgaaacca gaataatgac
    20521 catcttttaa tagtaatagg acgcacgata gatagaaaaa ggaagagagg agaggaaaac
    20581 aatcgtcctc tccatctttt tacctgagca acaagcaaac tagaaggggt aaaaaacttg
    20641 gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac agcggacaaa aaaaggagta
    20701 ctagcgggta tcttaggatt ggttacgaat ctgatcttgt ttgtctctaa gctaatgatc
    20761 gggctttttt caggaagtgt ctccattatg gctgatgcag tgaacagtct atcagatact
    20821 gcttcatcca tattgacttt gatcggattt aaaattgctg caaagccagc agatcaggaa
    20881 catccttatg gacacgaacg cttcgaatat atcagtgggt tatttgtttc gatcatcatt
    20941 acttatgtag gatttcaatt tttagagtct tctgctgaaa aaatcatcca tccagaaaat
    21001 gtctctttaa cacccatcgt cttttttgtc ctaatcattt ccatcttttt gaaatttgca
    21061 caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt ctgaaacatt gagagctaca
    21121 gcaacagata gttataatga tgtctttact acactggctg tattgctttc agcagcaatc
    21181 gaatgggtaa ctggatggcg tattgatgga tatataggtt ttgctttagc attatatatc
    21241 ctttatagtg gattgacgat gcttcgaaat tttgtatacg aactattagg aagtcgccca
    21301 acgccagcag agattgaaga aatggaagag catttaaatg cttgcgatga tatacttggc
    21361 tatcatgatt tattggtgca taactatgga cccaataaac gctttgcatc agtccacatc
    21421 gaagtagatg aatcaatgaa tttgaatgtg gctcataaga ttattgatct gatagaaaaa
    21481 gactttaaaa agtctttgga tgtggaactt gtctgtcact tggatccaat gcctgttgat
    21541 aacgaaaaat actataatat cttgcggcag ttaaagcaaa ttcttcttga aacaggtaca
    21601 gggcttaata ttcatgattt tagagtaatt catcaaggaa aaacgctgca atttgatgta
    21661 gtggtccctg agaaattcca gtttgttgat acagaactag aaagaatctt acgtagtcaa
    21721 atagaagaaa aaatcggaga ttaccaattg gaaattacgt ttgatcacaa ttatctttta
    21781 tattaaatga gaaactataa aaagactcct taaagttttt aatttaaaga gtcttttttt
    21841 cttttaattg tttatggaga gcctgacgtg caagattatc tgctccttta tttttgtttt
    21901 cgggcaacca ttgcaggatc aataaagaaa attcggatag aagttcttgg attctttgaa
    21961 ggtagggaag gaagagctcg ttcttcgttg cgtttttgtc gaccgtagaa actagtgttt
    22021 tgctgtctga aaaacagaaa atcgtttcat tctgtaagtc gtttttcttt agaaattcta
    22081 aagtttttaa aaagacttcg aactctgctt gatggtttgt ccctatagaa agcgggatag
    22141 atagctgttc ttgtttacct tgatagacaa tcaagattcc acctccacta ggaccagggt
    22201 tgccttttgt agaggcatca atgtatactt ttaacataaa aagagattct cctttttgtt
    22261 caaactaatg ttatggtaag attatatcat tactgaatcg gtgagaggtt agagaatgaa
    22321 aaaaaatatt cgatggcagc ctgaactggc acatacgatc atctattggt cattgacctt
    22381 gatcattctg ttttttagtc tgattctttc tttagaaaac acaggtcctt attggaaaag
    22441 caattttgtt ttaggtattt ttcttgtttt tctatttcta ggtttgcggc gttcttttat
    22501 tttaaaatca aactctgtga ttatacgtta tgctgctttt tggaaagacc gagaaatatc
    22561 tgtcggacag attcgatctt tgacgccaac taatactggg gtgcaaatga tgttgcaaga
    22621 tgagaaagcc ccatctgttt tcatgatgcg aaaaaaagat caagtagtcc tttttgatca
    22681 ttttgaaaaa aacggaccca aatcagaatc aattccttat ccttatacga aaaaaagaaa
    22741 aagctaattt taaaaatcag aacctaaaaa aacgaatttt tacaggaaaa gaaaagagtg
    22801 agacgtacgg ttcatcccgt ttagcgtctc actctatttg tttatctatt catgcatact
    22861 agtatcggta gtctgaacgt tgcctttctt gacatgggca gcttgtgttc ctcgattccc
    22921 ttcgatgatc tcaaattcga ccacttgatt ttcttccaaa gttttgaatc cctcttcttc
    22981 aatcgcagta aaatgtacaa aaatttcttc tgtgtcatca taactgataa agccgtatcc
    23041 ttttttgttg tcaaaccatt ttacaattcc tgtggtcaaa gcactcacga cctttctaaa
    23101 gtctgatcat agccttcatt ataaagagta tttataagta cgtcaaataa attaaaaacc
    23161 tgtaatttta tgtttttttt ctgaataaaa aaaggtataa ttggcatatc ttaacaaaga
    23221 agatattttg ttgaataaag ttcgtgaaaa ggggtaatca aatgaagaat gatctgcaaa
    23281 tttcacaaga aacagagcta gaaccgattg ttgaagtggc tagtaaatta gatcttaagg
    23341 aagacgattt ggaactgtat gggaaatata aggcgaaaat caatttttct ggaatcaatc
    23401 gatcaaagga taacgctgag gggcatttga tcttagttac ttccatcaat ccaacaccag
    23461 ctggcgaagg aaagtcaacg atcactgtag ggttaggaga cgcgctcaat aaaatcaata
    23521 aaaaatctgt tattgcattg cgagaaccct cacttggtcc tgtgatggga atcaaaggtg
    23581 gtgctgcagg cggaggatat gcacaggtat tgcctatgga agatatcaac ttgcatttta
    23641 ctggtgacat gcatgctatc acaacagcta ataatgcatt atctgctctt ttagataatc
    23701 atattcatca aggaaatgaa ttgaacatcg atgcccgccg agtgatttgg aaacgggtag
    23761 tcgatctcaa tgatcgtgaa ctgcgtaaag taattgttgg cttgggtggg ccaatccaag
    23821 gtgttccaag agaagatggg tttgatatca cagttgccag cgaaatcatg gctatcttgt
    23881 gtcttgcggc agaccttgaa gatttaaagg ctcgtcttgc aaggatcgtt gtcggctata
    23941 catttgaccg tcagcctgtg actgctggag acttgaaagc agaaggcgct ctggcattac
    24001 tgctgaaaga tgcaatcaag ccgaatcttg tacaaacaat ttacggaaca ccagcttttg
    24061 tccacggggg accgtttgcc aatatcgctc atggctgcaa tagtatttta gctaccaaga
    24121 cagcattgag attagcagat tacgttgtca cagaggcagg atttggtgca gatcttggcg
    24181 gagagaaatt tttagatatc aaagtaccta acttgaaaaa agcgccggat gctgtggtga
    24241 ttgtcgccac tgtccgtgca ttgaaaatgc atggcggaat gaaaaaagat gaacttaaaa
    24301 atgagaatct tgatgctttg aaagtaggtt ttgcaaattt aaagcgccat atacgtaata
    24361 tggaacaata tcagctacct gtgatcgtcg caatcaatga attcgtaaca gatacagaca
    24421 atgaactcac actgttggag cacttgtgtg aaaatcaggg gattcttgcc aagagagcga
    24481 gtgtatgggc agatggagca gaaggcggcg ttgatttagc aaaagctgta gtacgattga
    24541 ttgatagaaa agaagctaat tacaagccat tataccgcct agaagaaacg atacaagaaa
    24601 agacagaaac aatcgtcaag aaaatttacg gaggaaatgg tgttgttttc agcaaaaaag
    24661 cgcaaaaaca aatagaagag tttacgaaga atggatggga caaactgccg atttgtatgg
    24721 caaaaacaca gtattcattt tctgatgatc cttcactgct aggtgcaccg gaagatttca
    24781 ccatcacgat ccgcgaaatc atcccaaaat taggggcagg atttttagta gccttgactg
    24841 gagatgttat gacgatgcct ggtttgccta aaaaacctgc tgcattgaat atggacgtga
    24901 caaatgacgg agaagtgttg ggattgtttt aagcttgatt ataatctacc atcgaaggag
    24961 ctgtattttg actacagctt ctttttttag acgataggga attaattttg tttacactag
    25021 gatcattctt tttctttatt gtattttgag ctatactaaa gcataatagg agggtatgat
    25081 gaaaacgaca tttataagaa aaaccggttt ttacggtatg ggtagctcga ttcagctaag
    25141 aatcaacaat gaaaaacgca gtgttctcag tcatgaacaa aagataacgc ttgatctgga
    25201 agtgcccttt acgatgcagg tcacctttta ttggctaaaa agccccgttt atacagtgaa
    25261 agaagcaaag cctgcttatg tgatcacaat gaattacttg attcttcaaa tctatccgtt
    25321 tttatttcta gcaactgggt tgggggccgt tttcctacaa aatttatttt tcagtttttt
    25381 tgcagttatt gtgttgattg cgttcttttt ctatataaaa aaccgggcat atttgatcaa
    25441 ggaggaaaat tatggcaaac tctgatcttt ttttaatcag tttcaatcga atcgaaaaat
    25501 ggatgagaga cgagatggga aatgcacgaa atatgggatt taccgaactt gtcagacgcc
    25561 tagctcaaag aaaacaatta atgatcagaa aatacgaaga tgatctttta cagcttgctc
    25621 agttaaggaa tgcgatcgtc catgatcgga tcgcagtcga ttttgtcatt gcagagccaa
    25681 atgattggac agtcaagcga atccagctga tcgaacaaga actgattcag cctgaaaccg
    25741 tgctgcctcg atttgcaaaa cgtgtaacag gttttgaaca agatcttccg ttacttgaac
    25801 tgttaaagat agtagcagaa aaaaggtatt cacaatttcc gttatacaat aaaggaaaat
    25861 ttgtagcatt gattacttta cgtaatatcg ggttttggct agcaaaagaa agccaaaaag
    25921 gtccggttga tttaacaaat aaacgagcgt tggatttgat catccaaaac ggaaagtaca
    25981 caaattatca ttttgttcca gctaaaacac atatttacga ggttgaagcg atgtttcgtg
    26041 agcaagggac tttggaagcc gtacttatca ctaaagatgg gaatcccgat ggtaatctgc
    26101 tcgggatcgt acgacctcgt gatatttata aacaaataga aaaggattga ttttttgtta
    26161 attatttatt ggatattcag cgctttgcta gtaggagtgg atcagttggt caaatggtgg
    26221 atcactgata atctagcgct tggagagacg aaaaatctta taccaggtat tttgtcactg
    26281 aatcatattc gaaacactgg agccgcatgg agtatgctag aagggaagat gtggttcttt
    26341 actatagtga cactcattgc agtcgttgtg attctcacat tgatgattaa aaatcgtgaa
    26401 aaaggtaatc gctggttcat gattggattg agtttgattc tagctggtgc gattggcaat
    26461 tttatcgacc gtattcgtct tggctatgta gtagatatgt tccagacaga ttttatgagc
    26521 tttccgattt ttaacgtagc agatgtgaca cttgtatgtg gagtgatttg tatgttaatt
    26581 tatattattt tagatgaaaa ggatcaaaga aaaaaatgac agaacttaaa acgacgattc
    26641 accatgaatc tggtaggatc gataaagtat tgactaattt attcaccgat tacagtcgtt
    26701 cgcaaattca gctgtggctg aaaaatggag cggtttctgt caatggacag ccagtcaaag
    26761 caaattacaa agtaaaacaa ggagacgaga ttactgtctt gatccccgaa ccagaaacat
    26821 tagatgtggt cccagaaaat atcccgttga cgatcgttta cgaagatgaa gatgtagcgg
    26881 tgataaacaa acctcaagga atggtcgttc atccttcagc aggtcatacg catggcacaa
    26941 tggtcaatgc gctgatgtac catatcaagg atctttctac gatcaacggc gttattcgtc
    27001 caggtattgt tcaccgaatc gacaaagata cttccggact gctaatggtt gccaaaaatg
    27061 accaggcaca cgaatcatta gctcagcaat taaaagataa gacctctttg cgcaaatatg
    27121 ttgctttagt ccatggagag attcctcacg aaaaaggaac catcaatgcg ccaatcggcc
    27181 gttcgaagga aaaccggaaa atgcaggcga taagagaaga tgggaagtcg gctgtcacac
    27241 attttactgt attggaacgt ttcgaaggat ttactctggt tgaattaaaa ctagaaaccg
    27301 gaagaacaca ccagatccgt gtgcatatga agtatatcgg ttatccgtta gctggagatc
    27361 cagtatacgg accgaagaaa actttgaaag gcaatggtca gtttctccat gcgcaaatgt
    27421 taggttttac gcacccgact acaaacgaaa agatgatctt tgaagcacct cttccagaag
    27481 tttttgaaaa aactttagaa aaattaagaa aaaacattgc attttaattt agataacggt
    27541 atagtaatta acaacgaaag cataaaactt aatagaattc ctttaagttt agtcccgtga
    27601 ggctaagaag ggtgggagca aggatacgta tggtgcgcca taagtagatt gctcattgac
    27661 atagtatagc tatcagtgta tcagtatacg ctcagtctaa acattcctcc tgtcctctta
    27721 gggcaggagt ttttttatgc aaaaaagtag ggggaatgaa aatgcaagca aaagaagtag
    27781 tagatcaggt aaccatgaag cgagcattga ctcgaattac ttatgagatt atcgagagaa
    27841 accatagtat ccaagatatc gtattggtag gtatcaaaac acgaggaatc tacattgcat
    27901 caagaattgc cgaacgcctg aagcagctag aagatatcga tattcctgta ggagaactgg
    27961 atatcacttt ataccgagat gacaaaaaag aaaatccaga agaaccggaa cttcattctt
    28021 ctgacatccc agtttcctta gaaggaaaag aagttatttt gattgatgat gttttgtata
    28081 caggacgaac gattcgagca gcaatggacg cagtaatgga ttttggcaga ccaagaaaaa
    28141 tttcattagc agtcctggtc gatcgtgggc atcgagaatt gccaatccgt gcagattatg
    28201 ttgggaagaa cattccaaca tctcgggcgg aagagatttt agtagagatg caagagcttg
    28261 atggacaaga tcgcatcatg attttgaaag aggaagatta gaggagagag aaacatgtcg
    28321 gagaaaaaag gatttcataa tgatgacgtg gtgttagata tccacgatcg cccaacccca
    28381 ggacattggg taggattaag tttacaacac ttgttcacga tgttcggagc aaccgtgtta
    28441 gtccccatct tagtaggtat cgatccaggt attgcgctag tcagttcagg actaggtaca
    28501 ttggtgtact tatttactac taaaggaaag atacctgcgt acttaggaag cagcttcgct
    28561 ttcattgcag cgatgcagct tttgatgaaa acagatggtt atccagcgat tgcacaagga
    28621 gcagtcacaa ctggattagt ctatttgatt gttgcgctga tcatcaaaaa gatcggttca
    28681 gcatggctgg ataaaatctt gcctccaatc gtcgtcggtc cagtcatcat ggttatcggg
    28741 ttgggacttg cggcaaatgc agcaacaaat gctatgtata atcagggcac gtatgatttc
    28801 aagtatattg ctgtagcgct gatcacatta ggattaacaa ttttcttcaa catgtggcta
    28861 agaggttttc tcggattgat tccgatttta ctaggtatta ttagcggata catcgtagcc
    28921 ttgctgtttg gaatcgtgga tactaagcca atcgtagacg ctgcctggtt tgctgttcct
    28981 aactttgaga ttccctttgt acaatatgaa ccaaagctat atcttggagc aatcacaaca
    29041 atggcaccca tcgcttttgt gacgatgaca gagcatatcg gccatcttat ggtattgaat
    29101 aaactaacta aacgtaattt ctttgaagaa ccgggtcttc ataagacgtt gacaggtgat
    29161 ggattagcac aaatcgtggc agcctttgtc ggaggtcctc ctgtaacaag ttacggtgaa
    29221 aacattggtg ttctggctat cacacgtgtc catagcgtgt ttgtgatcgg tggagctgca
    29281 gtatttgcag tcattttagg attcgtagga aaattaagcg ctttgatttt aagtatccct
    29341 ggaccagtca tctcaggaat cagttttatc ttatttggag taattgccgc aagtggaatg
    29401 aagattttaa tcgagaacaa aatcgatttt gataaaaaga aaaatctgtt gattgcttca
    29461 gttatcttag tagtaggaat tggcggactc gtctttgaag taggcacatt tacattgtcg
    29521 gcaatggcat tagcaactgt acttggcatc attttaaatc ttatcttacc agaaacttca
    29581 cgaagcgaag aaaactaagg aggaaacatt atgatcatca aatcagaacg tatcagctta
    29641 aagcatttat tgaccgttga ggcacttact gatcaagaag ttatgggctt gatccgcaga
    29701 gggcaggaat tcaagcaagg tgctaattgg actccgcaaa agcgccagta ctttgctaca
    29761 aacctctttt tcgaaaatag tactcgcaca cataaaagct tcgatgtagc ggagaaaaaa
    29821 ctagggctag aagtgattga atttgaagca agcacaagct ctgtccaaaa aggggaaaca
    29881 ctatacgata cagtactgac gatgtcagca ttaggcgtag acgtagcggt tatccgtcat
    29941 ggtgatgaga actattatga cgaactgata caaagtaaaa caatccagtg ttcgattatc
    30001 aatggaggag acggaagcgg acagcatcca acccaatgct tgcttgattt gatgaccatt
    30061 tatgaagaat ttggtcattt tgaaggtctg aaagttgcca tcgtaggtga tatcactcac
    30121 tctcgcgtag ctaaatccaa tatgcagatg ttaaaacgtc taggcgcaca agtattcttc
    30181 tcaggtccta gagaatggta cgacgatgaa ttcgaagtgt acggacatta tatgccattg
    30241 gatgaactac tagatcaagt tgatgtcatg atgatgcttc gggtgcaaca cgaacgccat
    30301 gatggaaaag aaagtttctc aaaagaaggc taccatcagg aacatgggtt aacagttgag
    30361 cgtgcgaaga agttgcaaaa acatgcaatc attatgcacc cagcaccagt taaccgtgat
    30421 gtagaattag ctgattcatt agtggaagga ttacagtcgc ggatcgttca gcaaatgagc
    30481 aatggagtat tcgtcagaat ggcaatctta gaagcagtgc taaatggaaa agcgtaatta
    30541 taggaggatg aaaaaatgaa aacactcatt aaaaacggtc agatcaatac acgaaaaaat
    30601 atgacgacac cagcagaaat ttggatagaa gatgggcgaa tcaaagcaat cggtacagga
    30661 ttctcagaag ccgaatttga tgaagtgttt gatgcaaagg gacaattgat cacacctggt
    30721 ttagtggacg tccatgttca tttgagagag ccaggattta catacaagga gacgatcgaa
    30781 gctgggactc gctcagctgc tcgtggcggt ttcactactg tatgcgcgat gccgaattta
    30841 aatcctgtac ctgatacggc agaaaagctg agacaagtat atgacatcat tagaaaagat
    30901 gcagttgtga aggtattgca gtatgcgcca atcacagaaa atctgcgaag tgagaaatta
    30961 gttgatcaag aagcactgat tgaagaaggt gcttttgctt tcacaaatga cggtgtaggt
    31021 gtccaaacag ctgggacgat gtatctggct atgaaagaag ctgcaaaaaa caataaagcc
    31081 ctagtagccc atacagaaga tgaatcttta ttatttggcg gcgttatgca cgctggcaag
    31141 aaggcagaag aacttggtct tcccgggatc ttaagcgtaa ccgagtcttc tcaaattgct
    31201 agagatctgt tgctagctga agcaactggt gttcattacc atgtttgcca tgtttctact
    31261 aaagaaagtg tccgtgtaat cagagacgca aaaaaagcag gaatccacgt aacagcagaa
    31321 gtttcacccc atcatcttat tttgatcgac gaagatattc ccgaagattt cggcttttgg
    31381 aaaatgaacc cgccgctaag aggacgggag gacagagaag ctttgatcga aggcttgctt
    31441 gacggtacga tcgattgcat cgctacagac catgcccccc atggattaga agaaaaaagc
    31501 caaagtttca tgaaatcacc atttggtatc gtaggaagcg aaacagcgtt tcaactgatc
    31561 tatactcatt ttgtagaaac tggtcgattc acgttggaac aagtgatcaa ttggttggca
    31621 gttaaaccgg ctgagatttt tggtttgaat gcaggaacgc taacggttgg tgcccccgct
    31681 gatgtagcag tttttgatat tacacaaact tgcacgatcg ataaagaaga ctttttatca
    31741 aaaggggaga atacaccttt tatcggctgg aaagtaaaag gcgaaacaca aatgactttt
    31801 gtcaacggaa aacttgtgtg gcagaaggga gaataaaagt atggaacgtt tgcttatatt
    31861 agaagatgga acagttttcg aaggaaaggc ttttggtgca gatgctagtg tcttcggaga
    31921 aatcgttttt acaacaagta tgactggtta tcaagaaact atcactgatc aaagttttaa
    31981 cggtcaaatc attacattta cctatcctat ggtgggtaat tatggggtaa atagagatga
    32041 ttatgaatcg atttctccaa catgcaaagg agtagtagta aaagaacatg cacgtgtagc
    32101 ttcaaactgg cgaaaccaaa tgacgctaga ttcatttttg aaacaaaaag gaattcctgg
    32161 aatatcaggt atcgatacgc gcgcccttac tcgcaaaatc cgtgaagtcg gaacgatgaa
    32221 agctagtata gttgatgctg gagattcctt cgaacatgca tttgatcagc tgaaagcaac
    32281 cgttctagca accaatcaag tgcagcaagt ctcaactaac aaaccttatc caagtccagg
    32341 aacaggtcgg aaagtcattg tcattgactt cggcttgaaa catagtattt tacgagaatt
    32401 gagtaaaaga aactgtaacc tcacagtact tccctataac acagatgccg agacgatttt
    32461 agcgctatca ccagacggag tcatgctgac gaatggacct ggtgatccaa aagatgtgcc
    32521 agaagcaatc gaaatgatca aaaaaatcca aggaaaagta ccgatttttg gtatttgtct
    32581 cggtcaccag ttatttgctt tagccaacgg tgcagatacc tataaaatga aattcggaca
    32641 tcgaggatta aatcacccgg tacgagaagt agcgaccgga agaattgatt ttacttctca
    32701 aaatcatggc tatgcagtcg atgaagccac gattgatcca gaaaaactca tcgtaactca
    32761 tgtagaagta aatgacggaa cagtagaagg agtcaaacac agagattatc ccgctttcag
    32821 tgtgcagttt catccagatg ctgcccctgg tccgcatgat gcactacatt tatttgatga
    32881 atttatggaa atgatggacg caggaaagga gcagtaaaca gatgccgaaa cgtacagata
    32941 tcaagaaaat tatggtgatt ggatctggtc cgatcatcat tggtcaagcc gcagaatttg
    33001 actacgcagg aacacaagcc tgccttgcct taaaagaaga aggatatgaa gtggttttag
    33061 taaattctaa tcctgcgacg atcatgacgg ataaagaagt ggcagatcaa gtatatatcg
    33121 aaccgatcac acttgaattt gtatcaagga ttcttcgaaa agagcgtcca gatgctctcc
    33181 ttccaacttt aggcggacag acaggcctca atatggcaat ggagctggca gcatcaggta
    33241 ttttagaaga attgaacata gaacttctag gaacaaagct ttctgcgatc gatcaagcag
    33301 aagatcgtga tctattcaaa cagttgatga aagaattgaa tcagccaatc cccgagtctg
    33361 aaatcgtcaa tacagtagaa gaagctgtag attttgcaaa taaaatcggc tatcctgtca
    33421 tcgttcgtcc agcctttact ttaggcggaa ctggtggagg tatgtgtgaa aatgaagaag
    33481 aattacgtat cgtatcagaa aatggattaa gtctttcccc cgctacccaa tgtttgattg
    33541 aacgaagtat tgcaggtttt aaagaaattg aatatgaagt gatgagagat tcggctgata
    33601 atgcaatcgt tgtctgcaat atggaaaatt ttgacccggt gggtatccat acaggtgatt
    33661 ctatcgtatt tgcccctagt caaacattgt ctgaccatga atatcaaatg cttcgtgatg
    33721 cttctctttc gattattcga gcacttaaga tcgagggagg ctgtaatgtc caactcgctc
    33781 ttgatccaca tagttttaat tattatgtga ttgaagtcaa tccgcgtgtt tctcgttcat
    33841 ctgcgttagc aagtaaggcg actggctatc cgatcgctaa gctagctgct aaaatagcgc
    33901 ttggattaac attagacgaa atgaaaaatc ctgttacagg aacgactttt gcagaatttg
    33961 aacctgcatt agattatgtt gtagctaaaa ttcctcgctg gccgtttgat aagtttgaaa
    34021 acggagaacg gcttttaggc acgcagatga aagcaactgg tgaagtgatg gctatcggtc
    34081 ggaatatcga agagtcttta ttgaaagctg tgcgttcttt agaaatcggt acacaccata
    34141 tcgaacttcc tgagttggca gacatcacgg acgatgacct aatccaaaaa gtggttcgag
    34201 cacaagatga tcgcctgttt tatgtagctg aagctattag aagagggtac accatagaag
    34261 aattagcgga tttgacaaaa atcgatttat tctttttaga taaactgttg catatcgtag
    34321 aaatcgaaca gcagttgaaa cagcagccgt tggatgacaa gatattgaaa acagcgaaac
    34381 aaaacggttt tacagacaaa aaagtggcgg agctgtggga aataaacgaa gaagccgttc
    34441 gccagatgag aaaagaaaat gatttgattc ctgtatataa gatggttgac acttgtgcag
    34501 cggaattcga atcaaaaaca ccttattttt atagttccta tgaatatgaa aatgaaagtt
    34561 cacgatcaga aaagccctca attctagtac tgggatctgg tcctatccgt atcggacaag
    34621 gggttgaatt tgactatgcg actgtgcatt ctgtcaaagc aatccagcag gcaggatacg
    34681 aagcaatcat catgaacagc aatcctgaaa cagtttcgac tgacttttct atttcggata
    34741 aattatattt tgagccattg accttcgaag atgtgatgaa tgtgatcgag ctagaacagc
    34801 cgataggagt aatcgttcaa tttggtggtc agacagcaat caacttagcc gaacctttga
    34861 ctgctgctgg tgtaaaaatc ttagggacaa cgatcgaaga cttggatcgt gcggaaaatc
    34921 gtgatttatt cgagcaagcg ctgaaagagt tggatattcc tcaaccttta ggggatactg
    34981 ctacaagtaa agaagaagcc gtggttatcg cttcgagaat tggttatcca gttcttgttc
    35041 gtccaagcta tgtattaggc ggacgtgcga tggaaatcgt tgaaaaccag cgagatttag
    35101 aagattacat ggaacatgct gtgaaagcat ctccagaaca tccagtacta gtggaccgtt
    35161 acttgcttgg tggtgaatgc gaagtggatg cgatttgtga cggtgaaaca gtactcatcc
    35221 cagggatcat ggagcatatt gagcgtgctg gtgtccattc aggggattca atggctgtgt
    35281 atccacctca aacattatct gaatcgatca agaaaacgat cgaagattac acgatccgct
    35341 tagcattagg tttgaattgt gtcgggatga tgaatatcca gtttgtcatc catgaaaatg
    35401 aggtttatgt gatcgaagtc aatcctcgag caagtcggac tgttccattc ttaagtaagg
    35461 tgacaggaat tccaatggca caaattgcga cgaaagctat tttgggagag aaactaaaga
    35521 cacttggtta tgaaaacgga ctttatccag aaacaaaaga agttcatgtt aaagcacctg
    35581 tcttttcttt tacgaaattg cagaaagttg atacttactt agggcctgaa atgaaatcga
    35641 ctggagaagt aatgggatct gacagacaat tagacaaagc tttgtacaag gcattcgaag
    35701 cttctggatt acacttgcct gaatatggag ccgtattatt tacgattgcg gacgaaacaa
    35761 aagaagaagc actagaatta gctaaacgtt ttacagaaat tggctatagt ctgataggaa
    35821 cgaaacatac agctgcttat ttcgaaaaaa atggtctggt cgttgaaaca gtagcgaaaa
    35881 tttctgagga agcagcagaa aaaaatgtag tcgacttaat aagagatggt aaaacacaag
    35941 tggttgtcaa tacgattgat aaagatcggg gaaatgcttc taaagacggt ttcatcatcc
    36001 gaagagaagc agttgaacat ggtattccat tgtttacttc tttagacaca gcagatgcga
    36061 tcgttcgagt gatggaatcc cgtgcttttt caactaaccc tatttaacgg cgagcacatg
    36121 ttaagacaaa aagacgaaaa accgttgcag gaggggacag atgaaacaag aaatgatgga
    36181 aatcgttcag caaaaacagc tggcaccaaa gatttttgaa atgacattga aaggtgatct
    36241 agtaaaagaa atgcagcaac caggacagtt cttgcatatt ctcgtgccgc gtgcagactt
    36301 gctattacgc cgaccgatta gcttgaatca tatcgaccac gaaacaaata cttgccgtat
    36361 catttatcgg gtggaaggtg aggggactag agtcttctct actctttctg ccggagacca
    36421 tttagatgtc atgggtcctt taggcaatgg ttttgatttg tccaatcttc atacaggaga
    36481 cgaagtattt ataatcggtg gaggaatcgg tgtccctcct ctatatgagt tatcaaaaca
    36541 gttagtcgaa aaaggaatta agccaacgca ttttttaggt tttgccacac atgaagtcgt
    36601 ttattatgaa aaagaattta atcgtctagg agaaacaaga atcgctactg acgatggaac
    36661 atacggtatg cacggaaatg tcggtaattt attgttagga acgaagacgg aacctacagc
    36721 tgtttttgca tgtggttcaa acgggttatt gaagacagtg gaacagctgt tttcttcgca
    36781 tcaaaatgtc cagttatcat tagaatcccg catggcatgt gggatagggg cttgctatgc
    36841 ttgcgtttgt catacgccgg aagatgaaac aggaacaaaa agtgtgaaag tttgtgacga
    36901 aggaccgatt tttaaaatag gagaggtaat tatatgaatg agaatccttt agcggtcaaa
    36961 cttcctggcc tagaattaaa aaatcctatc atgccggcaa gcggctgttt cggttttggc
    37021 aaagaatacg gaaaatacta tgatttgaat cagcttggat cgatcatggt caaggcaacg
    37081 actccaaatg cccgctttgg caatcccacg ccaagagtgg cggaaacacc atctggcatg
    37141 ttgaatgcaa ttggtttgca aaatcctggt ctagacgttg ttatgcataa acttcttcca
    37201 gaattagagg agtacaatga gttacccatc atcgcaaatg ttgctggtgc ttgtgaagaa
    37261 gactatgtag aagtttgtag caaaattggc gaagcaccaa atgtgaaagc aatcgagctg
    37321 aacatttctt gcccaaatgt caaacatggc ggaattgctt ttggaacaga ttctgatgtt
    37381 gcttttcaat taacacaagc agtaaagaaa gtttcttctg ttcctgtcta tgtcaaattg
    37441 tccccaaatg taacagacat cgttccaatc gctcaggcaa tcgaagctgg aggggcagat
    37501 gggtttacga tgatcaatac tcttcttggt atgcggattg accttaagac aagaaaaccg
    37561 attttagcca atcaaacagg tggactctcc ggaccagcaa tcaagcctgt cgctattcgt
    37621 ttgattcacc aagtagcaag tatatccagc cttccaatca ttgggatggg cggcgtgcag
    37681 acagtggatg atgttcttga gatgtttatg gctggagcaa gtgctgtagc tatagggacc
    37741 gcgaatttca ccgatccgta tatctgtcct aaactaatta aagagctccc tgtgcgaatg
    37801 agcgaactag gcatcgaatc gctggaaaga ttaatcaaag aagtgagaga ggaacgtgaa
    37861 cgatgaatca actagaccaa cgaccaatta ttgcattaga tttttcaact aggcaagagg
    37921 tagaagactt tcttcgattt ttcccgaaag aagaaaaatt gtttgtgaaa atcggaatgg
    37981 aattgttcta ccaagaagga cctgaaatcg tacgttatct aaaagaggct ggtcataatg
    38041 tatttttaga tctgaaactt cacgatattc cgaatacggt cgaaaaagca atgcggggat
    38101 tggcaaagct tggtgttgat ctcacctgtg ttcatgccgc tggcggtatt cgtatgatgg
    38161 aggcggcaat gcgaggatta gaagagggga ctccagaagg tggaaaacgt ccgctacttc
    38221 tagctatcac tcagcttact tctacaagtg aagaagaaat gcatgccgat caactaatcg
    38281 aagtttcttt agaaaaaagt gtgatccatt atgctagctg tgccaaaaaa gcaggattag
    38341 atggtgtggt ttcttctgct tgggaagtag aagcgatcaa agaaatggca ggagacgaat
    38401 ttgtttgctt aactccgggt ataagaccag aaggaacagt cgcaggagat cagacaagag
    38461 tagtcacacc ttctcaggca aaaaagatcg gctcaacttt tatcgttata ggtcgaccaa
    38521 tcactcagtc aactaatcct tataaagcat atcaaacgat tcaaacagaa tggagctaac
    38581 ctaaaatgaa tgttgaacaa agtatcgcaa aagacttatt agaaattgaa gcagtatttt
    38641 taaatccaag taatcctttt acatgggcaa gtggaatcaa aagcccaatt tactgtgata
    38701 accgaatcac gatgagctat ccaaaagtcc gaaaagagat cgcaaaagga ttagcaagca
    38761 aaatcaaaga agcttaccct gaagtacaag tgattgctgg aactgcaaca gcaggaatcc
    38821 ctcatgccgc atgggtagcg gaaatactgg atttgccaat ggtctatatt cgaagtaagg
    38881 cgaaagatca tgggaaaggt aaccaaatcg aaggacgtat atttgaaggt cagaagatgg
    38941 ttgtgatcga ggatttgatt tctacaggcg gaagtgtctt ggaagcagct gaagcagcaa
    39001 aaagagaagg tgcggatatc cttggtgttg cggctatctt tacgtatgag ttgccaaaag
    39061 gaaaagcaaa tttcgaaaaa gccgaaatcc ctcttatgac gttgaccaat tattctgtat
    39121 tgattgaagc agctttagaa gatcgttata tcgatgagca agaattaact ctgctaaaag
    39181 aatggaaaaa agatcctgaa aattggcaga tttaagcgcg cttgatgaag atagagaaat
    39241 ccgatttgtg tctccagaat catcaattgg aatagcaaaa atccaaaaac tgattgcgag
    39301 aagcatcttt tcatcaggta tgaaaagaaa cttctatcgt gatcagtttt tgattttttt
    39361 aaaattcatt tttctaagac tggttagcag aaagaagaga ataatctctt ctcttttttc
    39421 tattatatat agattttctc tattttttgg taagggagct gtttcaaaaa tggtaaagag
    39481 cggaaatgtt gcgaatatca gttatttaat gatatgatat tcggaaaaag aaatctcaaa
    39541 gggaggtaac ttttatgggg ttgaatgaat ttattttagg attgaacaat ttagagacga
    39601 tcacaagggc acctggattt tttaaatata cagagcatac agttgcagca cattcgtatc
    39661 gtgtagcatc tattgcgcaa gttctaggag acattgaaga agcgaatgga caaccgatcg
    39721 attggaaact gctctatgaa aaatcattga atcatgacta cacagagcgt tttattggtg
    39781 atatcaaaac gccggtgaaa tacgcaagca aagagttaag agggatgctg caaacagttg
    39841 aagaaaagat gacggacgaa ttcatcaacc aagagattcc agctgagttc caagaagttt
    39901 atcgccgtcg gttgttcgaa ggaaaagatg aaaccgtaga aggagaacta ttagccattt
    39961 cagataaggt tgaccttctt tatgaatctt ttgatgaaat catcaaaaat aacccggaaa
    40021 aagtttataa ggagatgttc attgaggcag tgactacgat taaaagcttt gaacatcggc
    40081 cttctgttca gtatttcttt aaagagatat ttccggaact attgaaccag gaattttatg
    40141 gaaaagaggc gtttttagaa accgtctcaa cgattgtaaa gaaagaagaa aaataaaaat
    40201 gatatgagat gcagggggaa gtatagttga aaaagaatat ggatgtcgaa tggggctatt
    40261 ctggagacaa aggtccggaa aattgggcga gtttatgtga ttggtttgcg agaggtgcgg
    40321 aatttccatt gcagtcgcct attcatttga ccaaggggga agaaaccaaa atagaaggag
    40381 acacgctttc ctttcattat caaaagcaac ggtttactga caaggaattc aaaaacacga
    40441 ttcatttagt accttttgac caactcagtt atgtagagtt caagaaagaa cgctattatt
    40501 tgacagatat tcatttccac ttgccttctg aacatatcat caacggaaaa caggaagata
    40561 ttgaatttca ttttgttcat atgaacagca aaaaagagaa tctagttgtg ggtgtcatgt
    40621 accagctaat ggtacaggaa ggttggctgt ataatcaaga aaatggggaa agttggaact
    40681 tagaaaaaca tgagcattgg gtaaatccgg atgtgttctt tccggagcaa aaaagccatt
    40741 tccattatat tggctcattg acaactccgc ctaccagcgg gccaatccaa tggtttgtaa
    40801 tggatcatgt aggaaaactg aaccaagagt ttcttctccc cttcgatggt cagtatgctc
    40861 gaccgaacaa ccgaccaatc cagcccttga atggaagaga aatttattat ttcgaggagt
    40921 ttgagcaatg aatattcagc agatgaaata cgtggtcgct gtagcaaaca atggtagttt
    40981 cagagaagcg gcaaagaaaa tgtttgtctc ccagccaagt ctttcagctg gtatcaaaga
    41041 attagaaacc gagttaggga tcacgctgtt tactcgaacg aatcgagggg catatttaac
    41101 agaagaaggg caagacttct taaatcgtgc cgagaaaata ttggtccagc ttgatacatt
    41161 agaaactcac tatctaacga gtgaaaagag agacaatttt tcgatcgctg cccagcatta
    41221 tgatttctta ggtcctttga ccgcacagct actcgaggaa ttcaaagacg agatcaaaca
    41281 atttcggatc atggagacga caacagcgag agtgatcgaa gaagtcaaag agcagcacag
    41341 tgagatcggc attatttata tgaatgagca taatcaagct ggtatcagaa gatatttaca
    41401 acaaggagag ctaagtggcc gcagcattgg aaaattcaaa acgcatattt ttttaaggca
    41461 aggacatcca ttagctgaac acgcagagat caccaaagaa gaattgttat cttatcctca
    41521 agttcgtttt acacaggatg gaaataattt cccgtatttt tatgaagacc ttatcgagat
    41581 ccctgatcaa gaaacagtga tttatacgag tgatcgaggt actttgatga atatcgttct
    41641 tgaaactgat gcttacgctt caggatctgg tattgtgatt ggagggattc gcgagcactt
    41701 gcggttaatc cctttggcag aaggcgaact aaatgaattt tatatcattt attcggcgaa
    41761 acgtccgctc agtgaaatcg ctcaaagatt tatcaaagag cttactcggt tactggacca
    41821 gcagagctaa gaaaagcaga tgagaacgct gtttattgaa caccgttgat caaaaaagag
    41881 gggttgtgac agaattttgt cacaactcct ctttttgtag caaacaattt ataactaatt
    41941 gctgttgtgg gaaagtacgc aaatatgatt ctagaaacac tcattgatta cctgttttgt
    42001 ttcatagctg taattttttc ttcttctggt gtcacgataa tcgtcttttg gttttcataa
    42061 ataacataac caggcttggc gccatttggt tttcggatat gctttacctg tacaagatca
    42121 accggcactt gggcagagta tcggtatttt gaaaaatagg cagctaactc ggcagcttct
    42181 gtaattgttt catctgaggg cttgtcactt ttaatgatga cgtgcgaacc gggtatattt
    42241 tttgcatgaa gccaatagtc ggttttttta gctgtcttca ttgtcagctg gtcattttga
    42301 agattgtttt taccaacaag aatgagcgtt ccgtcagaag aaaagtattg atcaggctga
    42361 gatggtttct tacgtttttg tttttttaag ttcttctttt tcaggtatcc ttctgaagtc
    42421 aattcttctt taattgcttc gatatccatt gggccggcaa tttccagttg agataagaca
    42481 gattcaagat attggatttc atctttggct tcttggattt gttcgccaat cagcttaaca
    42541 gcgttcttca gcttttggta tcgatggaaa tatttctgtg cattttgatt tggtgtcaat
    42601 gccggatcta gtgctatttt gattggccga tcttcttcat aataattagg taaaacaact
    42661 tcttttgcgc cgcgtggtac ttgtgtcaga aaagtagtaa gtagttcgcc atttctacgg
    42721 taattctcag cgttttcgga ttctttgagt gtttgttcac gttttttcaa tttattttta
    42781 tttcgtttta gttcattttc gatttttcga atcagttctc cgccttgttg ttttgcccga
    42841 tctttctctg ctttttcgtg ataatagcta tctaataatt gactgagtgt tggataagct
    42901 gttacagcag atgcctgttc ggacaatacc tgataagcaa tcggtgtgaa aaaatcttta
    42961 ttttccgttt cgtaaaaagt aggaataggc tgatggctga tggctgagaa aaattcgtcc
    43021 catacaacca tcttttcatt aggacgttcc agtaatcttt tggtcagctc ttgagcgata
    43081 tcgaaaccga ttccctgaaa ctgacgttgg attgcttttg gatctagttc agtttgggaa
    43141 agccgctgaa agattttttc tttctcggag ctaaatgggt tcaagacctc ttgttttggc
    43201 ggagcaacat attctacacc aggaagcaaa gaacgataag tgttttgaga gcttccaatg
    43261 tgtttgatcg catccagtat ttttccagtt tctcgattaa ctaagacgat cgtactatga
    43321 cgtcccatta attcaacgat caatatgata ttttgtagat cacccaactc gtttcgttta
    43381 gcaaagtgga aatggatcac tcgatcattt tcgatttgtt cgatgtcttc taaaatagcg
    43441 ccatccaaat attttcgaag catcatcacg aagttaggcg gattatcagg attctgataa
    43501 tcgatttggg taatctgtac acgagcataa cttgggtgcg cagataataa taatcgatgg
    43561 tttttgccgc gtgaacgaat caccagcacg atttcatttt cataaggctg atggattttc
    43621 gagatccggc cagatagtaa cgtttcacgc aattcgttga ccatcgcgtg ggtaaataca
    43681 ccatcaaagg acatggttca ttcctcattt cttttcataa gtatattcat tataacgaaa
    43741 aaaagaagaa aaaccaattt ggccgaatag taaagcaaaa gtctagaaaa atcttggact
    43801 aattacataa aaaacagtgt ttctatcgtt gaaatccaaa tttttaggta taataaaaaa
    43861 taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa tcgagagtac
    43921 aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag tattttcgac
    43981 gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt ttattgccgg
    44041 aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca accagcaaat
    44101 tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg gcgatatcgg
    44161 tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt tttaatgatc
    44221 ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt tatgatgtac
    44281 atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt ttctttgatg
    44341 gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat tttgataagg
    44401 agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta gaagaagaat
    44461 tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac attggcgcga
    44521 tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat gtttgatctt
    44581 caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat tcgattagac
    44641 ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg tatttcagca
    44701 gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt gagactttcc
    44761 catagataca actttttaga gtgcatttct aaagcttgta tctatgctag acatgaacat
    44821 tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg accgctttgg
    44881 ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa aagattgact
    44941 ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa ttattttagt
    45001 ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa gtacttcgac
    45061 gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc atccggcatt
    45121 agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa aagaaggaga
    45181 aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag ccacaatgag
    45241 tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac ctgccgctca
    45301 atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa cggatcctgt
    45361 gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag gtgtttctga
    45421 tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa atgcaaaaaa
    45481 agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag cacaagcaga
    45541 agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt ccactaatga
    45601 gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg ttccattaga
    45661 taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag caaatatccc
    45721 cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa tcgggatcaa
    45781 tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga gtggaaaaga
    45841 aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga tcaatcaatc
    45901 tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag ccaaaatcat
    45961 tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt atcagctatt
    46021 tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta tcggatctta
    46081 gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt ctgtgtgaca
    46141 gctattactc atggggtagc gccgattgta gctacattat taggcgtagg tgctggcatg
    46201 atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt tattttagca
    46261 gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg aacaccaaat
    46321 ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg gttacctgat
    46381 tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc tgtactgcta
    46441 ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa cgaaacgatg
    46501 gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac tttgtcgaac
    46561 ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc agatgtaaat
    46621 aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga agtgatcttc
    46681 caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat catttatcag
    46741 ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa gttgttctcc
    46801 gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact gaacttaaaa
    46861 acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa aatggcagaa
    46921 aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt aaccttacaa
    46981 tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa agcacactgt
    47041 tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt aaagatcagc
    47101 tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt ttccaagacc
    47161 ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt gcacaaaaaa
    47221 gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa gattttttca
    47281 gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca gcgggtgaat
    47341 tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca gctcctgagt
    47401 tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa gcattaatgc
    47461 gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc acgcatcgaa
    47521 tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg aaaatcgttc
    47581 aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg cagttctttg
    47641 aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac ttgaaaactc
    47701 attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa acatatttgg
    47761 gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat tctttgctct
    47821 gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt cgtacttttt
    47881 cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga ataagatgat
    47941 ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca gtttatcccc
    48001 attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt tagctttcca
    48061 agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg atatgttagg
    48121 tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg aggtaagcca
    48181 ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc gtttgattgg
    48241 ctataatact gatggtgcgg gcttcatgca atccgtgcaa gaagctggga tttcgatcaa
    48301 aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca ctgttcaagc
    48361 agcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa cattcgctca
    48421 agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa aagttcgtga
    48481 ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc tagtcaatgc
    48541 aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata taagcatgat
    48601 gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac gtttattaaa
    48661 agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga tctaccaagg
    48721 agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga ttgaaaagct
    48781 gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac ggtctcaaga
    48841 aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa ttatcaaaaa
    48901 atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga aacgaggagg
    48961 aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga aacctctgaa
    49021 gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta tggaaaatga
    49081 ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt tgaaccaata
    49141 cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa ataaaaaatt
    49201 tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat cgtggaaaaa
    49261 aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat tttttattgg
    49321 taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa cctttcatgg
    49381 gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata cacgcggaga
    49441 agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt cccataagcc
    49501 gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag gacaaaataa
    49561 gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt tagtagaaaa
    49621 ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg aaaaagtgat
    49681 cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga atctggatgg
    49741 tgtagatatt aaaaaagggg gccagaagat ggatggacat gttcttttgc agtacgcacg
    49801 tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc aagtaatgaa
    49861 cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt atgcagctgg
    49921 taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga aaaacctctt
    49981 ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag acggttcgtg
    50041 gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc aaatgaatca
    50101 atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa gaagctgcgc
    50161 aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt cttatggtag
    50221 atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa aaatgagtcg
    50281 cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg tatcaccaca
    50341 tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa aaatctaatc
    50401 acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac gccagaagga
    50461 attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac aagaaatgaa
    50521 gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga gatgccactg
    50581 gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg catcaactgg
    50641 cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca agcagcaaac
    50701 ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg tatctttttt
    50761 ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa ttaagtaacg
    50821 gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca aaagcctatg
    50881 ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc gatccattgt
    50941 ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg gttgctccag
    51001 gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt ttcccagaaa
    51061 ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta aaacgagaat
    51121 tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt gaagccatcg
    51181 cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat ttacgtttat
    51241 ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc caacctggaa
    51301 agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat tggctgattg
    51361 gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt gatggcagtt
    51421 tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg caagaagaaa
    51481 tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg acaaacaaag
    51541 ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa gagttaacga
    51601 aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt cttgaagtta
    51661 cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt ttgcagacaa
    51721 agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct caaggattgt
    51781 acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca gaaaatgagc
    51841 atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata gaagaagtac
    51901 agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt caagaacatc
    51961 atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag ctttccaaat
    52021 tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt gctgcagatt
    52081 atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta aaaagcatcg
    52141 gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca aaaaagaaag
    52201 aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa ctgaaaagta
    52261 tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc acagcaatga
    52321 aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa gaagtagaag
    52381 gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag aagcattaca
    52441 tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt tgtaaggcag
    52501 tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa gggtacttga
    52561 gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc aaaaaattca
    52621 tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt tcagatgaga
    52681 ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta ggaagatgtc
    52741 caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc ttgaatccgg
    52801 aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa acgaatctca
    52861 gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact ggtaaaaaag
    52921 ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt tcttttccat
    52981 ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca agaaaaaata
    53041 atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat tccgaaaagt
    53101 gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa acgttggggt
    53161 aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc attcattttt
    53221 aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc taaagtatcc
    53281 tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt caccaacgtt
    53341 gattttatcg actttgactg caggagctaa aagcgctact tatttttttc ataaaacgac
    53401 aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag cggctatgat
    53461 cattatttat tttctaccag ttattacaca agtatttcat caatattttg gttaagaata
    53521 cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt ttcgtaagaa
    53581 aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata atgtacttac
    53641 tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa agtataaaat
    53701 tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag aattcatggg
    53761 tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg tacgaatgat
    53821 caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga atcaatatga
    53881 agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga aattatcaaa
    53941 atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt aaaagatgca
    54001 aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca agaactctat
    54061 cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa acatcctttt
    54121 cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca attgaataat
    54181 tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat taccactagt
    54241 tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca aaccgcaata
    54301 aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta aggaatatgc
    54361 tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg aaaatatgga
    54421 ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag gaaatagtgg
    54481 tttagaacta gtgggctgat tttttatata ttttagttta cataatatat attatacaaa
    54541 gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat cgtcattttt
    54601 ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct tacttttttt
    54661 gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat atgaacgaat
    54721 tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac
    54781 attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg
    54841 atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct attagatatc
    54901 aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat
    54961 attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct
    55021 gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc
    55081 gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata
    55141 agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt
    55201 actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt
    55261 acggacttta tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac
    55321 aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc
    55381 accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc
    55441 gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt
    55501 tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac
    55561 aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga
    55621 cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt
    55681 gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt
    55741 ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg
    55801 attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat
    55861 acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa
    55921 taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc
    55981 attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc
    56041 tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc
    56101 aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc
    56161 aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
    56221 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
    56281 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
    56341 tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa
    56401 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
    56461 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
    56521 tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga
    56581 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
    56641 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
    56701 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta
    56761 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
    56821 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
    56881 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata atttgtaaac
    56941 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
    57001 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga
    57061 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
    57121 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt
    57181 tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact
    57241 gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac
    57301 ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt
    57361 acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag
    57421 tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta
    57481 cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg
    57541 tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg
    57601 tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt
    57661 acagtattgt cttagcttca agagctggac taggtattgg ctttggattg atcaattctt
    57721 tggcagtcag catgattgct gccttttatc acggagatga acgagcctct atgattggtt
    57781 ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag
    57841 tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct
    57901 tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc
    57961 caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc
    58021 tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt
    58081 ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg
    58141 ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt
    58201 ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga
    58261 ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca
    58321 accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg
    58381 ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt
    58441 ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg
    58501 cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg
    58561 aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa
    58621 atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac
    58681 tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg
    58741 atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt
    58801 ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg
    58861 gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat
    58921 cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag
    58981 caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa
    59041 aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga
    59101 ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat
    59161 ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta
    59221 tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta
    59281 aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag
    59341 tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac
    59401 tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta
    59461 tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga
    59521 tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag
    59581 gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga
    59641 ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc
    59701 ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt
    59761 atgggagagc aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc
    59821 ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata
    59881 tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat
    59941 atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta
    60001 tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt
    60061 tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg
    60121 acactatcac acagattact actccatatt tcaaattcta tgaacaagat gctttatgca
    60181 aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat
    60241 ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt
    60301 acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa
    60361 tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat
    60421 ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag
    60481 atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag
    60541 gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct
    60601 taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga
    60661 ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg
    60721 ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag
    60781 taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc
    60841 aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag
    60901 atctcctaat actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc
    60961 agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc
    61021 tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat
    61081 agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt
    61141 aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt
    61201 tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag
    61261 gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt
    61321 gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac
    61381 atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact
    61441 tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca
    61501 tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt
    61561 gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa
    61621 gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac
    61681 tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct
    61741 tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg
    61801 tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg
    61861 agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc
    61921 agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc
    61981 tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga
    62041 gggctgactg acgattgaca caaacaaatt caacaattta tccattgata gttcctccct
    62101 tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt
    62161 caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca
    62221 aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat
    62281 gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca
    62341 tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat
    62401 tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct
    62461 tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc
    62521 tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc
    62581 agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct
    62641 ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc
    62701 acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga taatagcggc
    62761 tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt
    62821 tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg
    62881 ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg
    62941 atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc
    63001 agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga
    63061 tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata
    63121 atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa
    63181 aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca
    63241 cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg
    63301 atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct
    63361 attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga
    63421 tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag
    63481 gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg
    63541 gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt
    63601 gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc
    63661 tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg
    63721 actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt
    63781 cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga
    63841 agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg
    63901 gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga
    63961 gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca
    64021 gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg
    64081 atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa
    64141 acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta
    64201 tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat
    64261 ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc
    64321 gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt
    64381 gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct
    64441 agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt
    64501 acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt
    64561 ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt
    64621 cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg
    64681 attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc
    64741 tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc
    64801 accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct
    64861 taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat
    64921 actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa
    64981 tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca
    65041 tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt
    65101 aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat
    65161 aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa
    65221 agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt
    65281 gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct ttgatgcgga
    65341 aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga tcgaagtaat
    65401 cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca tctggttcgc
    65461 cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa tagctgttcg
    65521 cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca taatgcattt
    65581 ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact gtcaagaagt
    65641 cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct tttgctaatg
    65701 caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact agtacattct
    65761 taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt gtagtgatga
    65821 caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta tattcgatgt
    65881 cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata ttgattttgt
    65941 tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca ttgccaagat
    66001 aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat atcctagaaa
    66061 agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa cacttgttat
    66121 cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag atagtagcgg
    66181 aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa
    66241 ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta catccgtagg
    66301 aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac gtatacttcc
    66361 cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa agaattccgt
    66421 ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct
    66481 aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta
    66541 tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc
    66601 ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc
    66661 acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat catggctgaa
    66721 gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg cgccattttt
    66781 attagctaca gcggtctcgc atccatcatt atgaacgaat aatgacctat ttgaagcatg
    66841 tagaggcccc taccttgctg attaccggaa atcagcattc agaaatggcc aaacaggctg
    66901 atatgtgtct tgttatatcg caggaggaat atgattttgc taaagtagcg actttctcct
    66961 ctcaaattgc cttcgattac gtattaaata cgttattctc agtgatatat tctcaaaatt
    67021 ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta
    67081 aagaatatcg gaaaacttaa gaaaagatcc tcatttagta ataagaaaga tagtgtttga
    67141 taggctctct ttgatatcat cataaaaaca ggctgaagtt ttctttttca aacctcagcc
    67201 tgtttgttta cggaacgatt cgaatagata tattatagaa atgaggaata gctaacttct
    67261 gtttcttatt ttgaacgatt gacgaaaaag atatataaga aggaggaaaa tcagtataca
    67321 tgcagctaac aatccataca ggaaaaatcg tttgttctcg gatactttct gtacttcttt
    67381 ttgaacttct tttgcatgat aaggaacccg gtgccctctg acaagtaagc gatgactgtt
    67441 gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt cgattctcag
    67501 atcttttgta tcagtcggtt ctaccgtctt gatctgatcg acttgataag cgagtgtccg
    67561 tccattgatt tcgatataga aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa
    67621 ctttgcttga ggcagtcctc gatggctgga aataacggca tgtgtatttt ctccccccgt
    67681 aggataagac gttccttcca ataatgaggc acctttttct agcagcacag tattcgtttc
    67741 atcaaaaatt ggcaaatgga cattgatctc agggatcgtc aaaataccga tcgtatgttt
    67801 ctcaaaatag gttctatcct ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc
    67861 tggatttcca ccttcttttg ctaattgctg gtttttcttt gtcatgtttt cttggatttt
    67921 agccatgact tcttcatttt ctttgaccgc ttgttgctga taatgagaga tgatctgctg
    67981 atccagataa ttattcagcg cgtcgctaac aaatggataa gctagtatcc ctattcctga
    68041 gagaagcaag aaaagcatca gcagatctag aagtttcctt ttctttggtt tcatttgtgt
    68101 tctccttgtg atgactgttt gataataaaa gaaaaaggag catttgcgta atccgctgct
    68161 cctttttcca attcttgcaa aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca
    68221 gtccagcaat cagtagaagg actactcctg caccgatata cacatagatt cccttaccgc
    68281 ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc
    68341 ctgctgttac ataagattgt tcatcgatag taaatgctac acggtttgtt aatggaacat
    68401 atttttctgg cgctttcgtt tcttccagat agtacgtgcc atattttaga cctgtcacat
    68461 cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc
    68521 tgacggcttt tgtggatggg tcgatcgata aatatttcgc tgtgtcacta tcttgatcac
    68581 gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca tctactttaa
    68641 caaatcgttt gcccccagta acgacatcga ctgacggtgg tgtttgatca tttgtatgcc
    68701 cgttatcgac atttgcttgg ttagaaaatc ctttggttgg atctgctttt tcgttcaaat
    68761 gcatatagta aacgaatttc aatgtaccgc caggagttaa tgaaggaata taattcggat
    68821 caactgaaac cgtgaatccg tctgtttgct cagtgacaga ataatttact gggtcgattt
    68881 ctttatttcc atcatataag gcataagcat acgttcctga agaatcatta tcaaatgtta
    68941 aagcagcgtc atgagtatcg ataagtttga atgttgtgta cttgttttgc gttccttctt
    69001 tatcagcgat acctaatggg atattgacag aaatctcata ttgaattttt tcaccgattg
    69061 cgacatcttt tccattcaat tgaggtgttg ttttatcaac tttagacgta tcatttttga
    69121 tggtcttttc tactggtgtt tggctatttg ccaggatctc aaaaggcgtg attgtttgct
    69181 tttcaatcat ttccgcatta tcaggagctt ttacttcttc taaattatat tttccaactt
    69241 ctaaatgatt aacgatcaac tggccttttt caatagatgc ctcggcaaag tcattgttgc
    69301 cgatgtcata agaatgacca gtaatgaaat gtttggcttt ggtttgatca gttgtccaag
    69361 tgtacaatcc atctgtgaca ctttggatgt attttttgac gcttggtgtt ccttcttctt
    69421 tagaaataat aaattctgct ccatttagtg cttcgttttc ggcagtaccg atttttgtaa
    69481 ctttcaacgt tccatcatta ccgactgtat ttttagggta gagatggata gtatctagtt
    69541 cttctgtccc gtatttataa gagccatctg cttgtttgat catctcatat acagggaaag
    69601 ctaaaaccat gtttgcggca gctgacactc cgtcttttgg ttcttctttg atcgtgtaga
    69661 ctgcatcttt cccattttgt tttttaggta aagataaagt gacatttcca tcagcatctg
    69721 tcgttcctga agcaactggt gtaccaggag tcaaagactg gactgcttgt tttgctgcat
    69781 ccacggacgc tcctttatct cgttgcgcat aaaattcttg agtgacgtta taaactgaaa
    69841 atgaaatatc ggctaatcct tggtattgat cgaattcgct catttctttc ccgctgtttt
    69901 ggattaaagg atcgggtaaa tcagtcattt tctttttatg aaggatgact tgagctgctg
    69961 tctcctctgc aaacactttt tttgcgccga agctgtttgt gagtaatggt aaaataagga
    70021 aaaggactcc taacataacg tttatttttt tatgattttt cattttttca ctcctgttct
    70081 ttttttgata aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag
    70141 tgtatttttc gtttcgtttg tttttggaag aaatggacgg ctattttctt ttttattgac
    70201 tactttcaat atactgtccg cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg
    70261 aatttctgtt tcacttaaaa tataaccaga aggtgcttcg atctctttta atcgataact
    70321 tcctgttttc aacccggaaa ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc
    70381 atctgattcg ttctttgtcc acttgtatcc gttggctgtt tgctggaggt aatttcctgc
    70441 tttgttttta ataaggaata ctgcgccagc taatcgttta tctggtttat ttgtctcgat
    70501 tttgacaaat tgcttactcc ctgtatatac agagactttc tttgtactga caccaaaacc
    70561 aaaatccagt gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg
    70621 catctgataa gaaatggtca gtttttttcc agcatatttt tccaacgaag ggatcgaaaa
    70681 taaaacatca aatccgtgag gtttcttgtg tatgacaaag ccttctgtca gcttttctcc
    70741 attcgatgaa atcgttaaac tattaggtaa aaacgttaat gcttcatccg cactatctga
    70801 caattcgaac ttttgatagt caaggatatt tacaggaatt gtcgtatgca aggaataagt
    70861 gattttttca ccaatcgtaa aatcatttct aggctcttct aataccttct caaaaggtgg
    70921 atcagggtag tcgttttctt catttttagg atacaaatgg atagttgaaa gtttttgatt
    70981 gttttgtcca tgtacaggaa gaacaactac catattctct gccttttctt tgacgacttc
    71041 tggtactttg gattcaatga aaagatacgc tttatctctt ttttcttgat ctttagcggc
    71101 cagagaaaaa gaagcgatac catcttcgtt gttaagagtt gtagttgttg cttctgcggt
    71161 aaacataccg gaagacgcac cgttttttgc gatctctgct tgtgcttctt ctaccgtttt
    71221 gcccttttcc cgtagatggt aaaaagaatc tgtgacatca taaacttgga atgtgacacc
    71281 atttaatcct cgatacgttt gtaataaagc tttttcttgt ccgtcatttg gatgattttt
    71341 cggcagttgc ccgtttggga aaagcaattt gtgtaaagta atctgaacca tttctgtttc
    71401 tgtttctgtt gctacctgag taaaagctgg tttaaatagt aacaagaaga gacacatact
    71461 aagccaacca agttttttca tatttagctg ccccctcttt cttttttcaa aaaaagatac
    71521 caaatagaaa acgcacaacc aatcatccct actagataga ttcctaaacg gccaattcct
    71581 cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt
    71641 ttggctcctg gtgacagaac agattcatga tcttgtccat ccacttgaat tgacccatct
    71701 tcgtgtataa ctatagtaac tggctctttt agaccttggt atccttctgg tgtaaaagtt
    71761 tcggtcagcg tataagttcc aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt
    71821 ccatcttttg gcgattctaa gtccatttct ggtccctgca gtctgaattt tgctcctttt
    71881 aatgtctgtc cgttgtcttc ttttttgtgt accgttagat caaacgcttt tagtcgattt
    71941 tgatgagtga gcgtccatac attatttgtc atctcggtat tttttcccgt gtaaatgatt
    72001 tttccatatt tatcgatttg gaattcataa tttcccggcg aagtatcata tccggcagga
    72061 ccagctgttt caacgactcg atatttacca ggtgtgagtg aatcaaaact ggcaagacct
    72121 ttttcattcg ttgtttggct gtcaattggc tgataagtac catttgcttc ttttttctgt
    72181 agggaaaatg ctgctccttt aagatttatc tctttgtccg tcccttgcat cgcatactta
    72241 cgtactgcta caggaacttc aacaaaagga ttttcaatag tcaactggat cgtatcatcg
    72301 gaagtagtga ctgtttttcc atcaatggtt accgtaccat cagaagcgat cttgatttcc
    72361 caagtagtct ttttgcttaa tccatgccct tctggagctt ttgtttctgt cagcgtatag
    72421 gtcatttctt tttgcaattt gacattttct ggaagagaat aggttccgtc gccatgatct
    72481 gttagtaaag tatcaataga atctcctgtt aatttgaaaa cagcgccaat aagatccttt
    72541 tcacctgtag aggaattttt cgttattttc aagtttaacg gtgtgaattg tttagtattt
    72601 ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt
    72661 gtttggtaac tgaatgcttg accttgatta ttgtattgag gtagtgataa tatctcttga
    72721 taactttctc cgctatttgc agataatttg tcaatgtctg cacgttccca cgtatttgtt
    72781 gtatctttag ctggtttaat gattcgaata tatccgtttt tccaagctgc agcatttgtc
    72841 gtatgttccc gttgaatctc aaaagtaact tgatctggac gatcagctag attgttgtca
    72901 aattcttccc ataacttttt gatgtgaaga ctgactccgg gagctttagc agatggtata
    72961 ccgaattcag ctaattcatt ggtatcaatg tttggctgga aagttgtcct gccgttcatt
    73021 tgataccaga aatttggatg gaagtcctca ttttctgttt ggattctcac ttggtaatgg
    73081 atttggattt cttggtcttt tcctaaatag atctgattgc tcttgatggt atttccttct
    73141 atggaaatag atggagtgac cgttgtagga cttgtcccca cactcttgac tgaaagtgta
    73201 ccaggctgat aaatgaatgg ttctgcgatt ggatcattta tttgtccatt gcttacagtt
    73261 gctgagatct gtacagcttt tttggctagg tattcagaga tatctgttgc atgatcagct
    73321 gattcatagt aaagatcgcc tttttcatct gatgaaacca tttgtcgcat acgagactct
    73381 acttctgctt ttgagagacc agctgccgga tcgctttgaa gttggatgcc aagaccatgt
    73441 atttcgattc ctcgttcttt gagtgccatc gcttctccga tggttgcgat aaacgtactg
    73501 tcgattcttc tggatagatt gttttggtca ggtgcatcat agattcttga gattagagaa
    73561 gtatttcccg gccgatcttg cgtattagaa aactgagttc cgtaataatt gctgcttgat
    73621 tgtgcgtgta cccgctgtac tttataggaa aatgttggta caccatccgt cagcaaaacg
    73681 atcacttttt tatgtccatt tggaacggat agcatgcttc ctgcatctct tagtgctttt
    73741 tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg attttacttg atttttcact
    73801 gaatcaaatg aacccatctg tactgcaccg ttactgtagc tatatccttc gcttgagtag
    73861 ccgacatatc ccatattgat tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa
    73921 cgatcgacac caatctttac ttcaccgatc cgattattgt cgttcatact tcctgaccag
    73981 tctacgacca atacgagatc aagaggcgtg atatcttttt gtacatttcc acgagcattc
    74041 aaataaacat caaacaatcc gggtgtactt gtttcctttg catactttcg tatcgcatag
    74101 tctgcttgat taccggttcc tccatattcg atatacgaat gggtccgatc attaggatct
    74161 ccattccaag aatttatacc atcccattga tttgttccgt ttttattgcc ttgatgatca
    74221 agaacatttg tatttcctgt aggttgccaa ctggctttag gataagtgcc ctgatcatct
    74281 gttgtatatt ctgtaggaat cgtttgatta gatggcacac tcacagctgg caaggataat
    74341 gtaggcaatg taaatgggct gacagaattt tcgttttctt ttgatgattc ttcttctatt
    74401 tcgtctggct ttggtactga cacatctgta tctttcttat tttctgtctc atcaataatg
    74461 tcatattctt tattatcgac gatcagcttg tttgcctggc tgtggtaaag tgctggatcg
    74521 attggaaaac tcagatcgat agtttgttta gtgtttgatg agatttccaa cgtaattttc
    74581 ccgctattta cctgataagc atagccatct ccttcttttc tttccacatc acttaagtaa
    74641 ttttcgacta gaccaatttc tttttctatc ttttcagcac tggtgttatt cgcatttagc
    74701 ttgatgttca gtgctttttt tccagactga tccgaaatga cttcagctga aattgtttct
    74761 aaaactgttg gatcatctgt agtgtctgct attgcagata atggcacaaa gtttgataaa
    74821 ctcaatatta tcagcatgaa aataactttc agtttcttcc ctgtggtcgt catttttccc
    74881 ctcctttttc tttttttctg ttttgacgtg aatagtgaaa attattaaaa taaacaggcg
    74941 ttacataatt ttatttattt taatatgata attattatac gaagggcgct aaagggaatc
    75001 acaccaattt tcataccaat tggtttcatt tatgaagttg attccttcga ttaatgagcg
    75061 tttcattgaa agaaaagggt tgagggagaa aaacatatga tcaggttgtt ggaacgaaac
    75121 tatagaaaga tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg gatgactaaa
    75181 aaagaacttg ccaaacgaat tggtagttca gaatctacat ttgtaaggta tatcgaagag
    75241 atcaaaatcc gctggggaac ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt
    75301 cttgaacatt tgaatgtacc agcttattta aatgttctga tggaaatgtc gcaaagttct
    75361 acaaacagtc gtttattaaa tgagattatt gtcaatccgg gtaaaacgat tgagtattat
    75421 tgtgaaaaaa tttccatcag ccggtcttct tttacacgta agttgaaaca atgcaatcaa
    75481 attttagaaa gctatttatt aaaagtgatc gttgaccaag gttttcagtt aactagcctt
    75541 aaaagtgagc tccagttgcg tatctttgtt acctttttct ttttaagtta ctacggaaga
    75601 cgcacgttgc catttccaat cgaaaaaaag aagatcaaag aaattatggt caagaataat
    75661 tgtcagatga atttgtttca ggaagaaagc ccatatgagc aatctttctt tattatgtat
    75721 tttgttgtgc atctcatgag agaatcccaa ggaactccat ttgaatggaa agctggagac
    75781 tcttatgtac agttgataaa tatcgataaa aaagacttct taatcttgaa aaatctctta
    75841 ggaaaattga agtacagcag ctatataaaa gtagtgaact attttgtcaa tacgattttt
    75901 tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc
    75961 tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta
    76021 ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg atacgactca tttgacgatc
    76081 cgtgcaactt actttgcaca gcagatgcaa caccgccaga tacgtctata tgctggattt
    76141 cgtgcagaac ttcaatttta ttccactcta ctgaatcaga atctgattta ttatttatct
    76201 agctatatct tttggatgct tatcggtaat cctgaaattc accattatac ttctgataaa
    76261 aaaatactga ttatcagcga cttaagtctt cgacatagtc aatatattga agaatatatc
    76321 agtgatattc ttgctgtgca taaaattcac tctgagacaa ctgctattac tgaagatcag
    76381 ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta cgaatcaacc cattttgaat
    76441 gcccatgttc ctcatatatt gattgatgat agtgtgtcat tcgcaaatga agaagaactc
    76501 atccgcttat tcgagttatg aataatcaaa aaacagctat gtataaacca ccctgataaa
    76561 aatttcagga caaatacaca tagctgtttc tttttattgt attccttcag caatccaacg
    76621 agatttatag cgaaagtctt ttaacaactc ttcttttgtc caattatttg caatgagatc
    76681 cgtcaaagtg ttcattacga gggtcatgga aaagtatagg aaactaactg cttcttccga
    76741 cgattgaatg gagaaatgat tgattcttaa taattccagc catttttcgt tcattggaga
    76801 atcagccgat agttctctac gtttggttgt ctgctgatca tttcgctggg ttaacaagta
    76861 taacctttgc caatagatac tttcgtgatc taagtcgcag caccactcta aatatttttc
    76921 gatccccaaa aagaaatgtt gtttgttttc gctaatgaat tttaagatat cttgatggac
    76981 tatgtgagaa ctgcttttga tcgtatacaa ataagcatct tccaaatctt gaaaatattt
    77041 ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt tctgagactt tgacttggct
    77101 gatgtgctgt gagcaaaata tctccagtaa taggtcatcg attctctgtt tctttgcgtt
    77161 cgataaattg aaaaaagttt cttttggcat ccacatctct cctttatctc tgattatacg
    77221 tggatagccc ttcattgtca tgaaggattc attccttctt tttctcttct tctgtcaagt
    77281 acccatcttc cattcgataa acgcgatcac tccattgaat catacggaca tcatgagtaa
    77341 ccataatagt cgctttttgt ttctcatgag cttctttaac tagtaatttg acgacttcat
    77401 atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc taagatcaag cttggctcat
    77461 tatataaagc gcgagcaatt gccactctct gtctttctcc accagaaagg tctttaggat
    77521 aactattttt aagtgactgg atatctaaag attgcagcaa ggaatcgatt tttgttgaat
    77581 cttctttttt tgaagtaact tggtcaacta gttcaaattg ttcctttacc gttaaaaaag
    77641 gaatcaaatt agagctttgg agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt
    77701 ttttttctgg aagctcagtt agtgagtgac cgttgatcac tatttctccg gaagtcggtg
    77761 tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa ggaccaatga
    77821 tgctgacgaa ttctccttcc tttacataaa aatcaattgc tttcaaagct gagacctttg
    77881 tatgaccaga accaaatgtt ttggtaactt ttttcatcgt taatattttt ttcattttta
    77941 ttaacctcct attgctgtaa ttgggtctac tttggttatc gtacgtatcg aaaacaatcc
    78001 gcctagcatt gcgacaatga tcaaaatccc actatatagc aaccattgtg accaaaaaac
    78061 agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca aaagagcaag
    78121 cagaacgcct accaccccaa caataaagga ctgtgctact aaagatttag cgatgaagaa
    78181 atttttgatc ccttgtgctt tcattactcc aaaaatagca gtcttctgaa gtgtcatcac
    78241 gtacatgaaa ataccgacca cggcagcaac cactaagaac aaaaagtata tcatcgcatt
    78301 taaggtcata ttttgggcag aatagccagg aatactttca atgaatgtcc caatagttaa
    78361 taactgtaga cccttcgatg ctttttcttt cgaaatttta gctgaatcct ttattacgat
    78421 cccattgatc ggctgttctt cttcagaagc aaacggctgt tgtccatact tcaaagcagt
    78481 ccacgtatct agggacgcat agatgacggg agaaactgtg taataagttt caggaaagat
    78541 accgacaatc gtcaaaggat cgtcgtaact gccaattttg atcgtatctc caattttata
    78601 gcctgaatca gccagatttt gagagatgat gatttcattc tttgttttaa attcatgccc
    78661 tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg taacattttc
    78721 tttgtttttt cctttgattg ctccgctgta caatccgatt ggtgctttct ctcctccttc
    78781 aatgtatttc agatctcctc ttgttaattg tgaagctgca aatacttgat ttgcatcctc
    78841 cgacaaaaca atctcttgtg cgtcccaatc atcgattgct tttttaaact cttctgaaag
    78901 tccattcgct agtcctgaca acatgaatac gacatacgca atcaagaaca taataccgac
    78961 gatcaagcca tatcgtaatt tagaatagcg aatttctttg atagctagaa acatttgttt
    79021 ttcctcccta tgatagattg atagtgacaa aatgtcactt gttgaatagt gtaactctaa
    79081 agtgacaatt tgtcacttgt gttgtttttt tcttatcaat aaattgtcta agacatcttt
    79141 cgataaaacc ttaacatgag tcataaaaaa tttatttaag ccttatttat gctttccatg
    79201 ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat taaactatta gtagtagaag
    79261 acgataaagt attatccaat aatataaaag aaatactcgc tgcaatcggc gagataacac
    79321 aagtttatga tggtagtgaa ggactgttcg agttggaaag tggagtttat gatcttgcaa
    79381 tcttggattt gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa atacggaaag
    79441 aaaaaataca gactccagta ttgattttga ctgcaaaaga tagtttggaa gataagattg
    79501 aaggatttga acgaggtgca gatgattatc taacaaaacc tttctatcga gaagaactga
    79561 tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa aatgttctga
    79621 agtataagga tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt
    79681 tgcccataca aggaaaagag tttgaattgc tcgtttattt tttacaaaat aagggaatca
    79741 ttttgacaaa agagcagata tttgaccgga tctggggatt tgattctgat acgacgatta
    79801 cagttattga agtttatatg agcaacttac gaaaacatct gcgaagtact ggattggata
    79861 aagaaatcaa aaccttacgt aatgtcggtt atatactgca aagggtgtga tgcaatgaat
    79921 ggagatatta cacgaaagca acgtagtcgc ttttttgcga caaatgtagc agcatttgct
    79981 tttatctttt ttcttttggg attcattact ttacagatat taaaccaatc tgcatacaga
    80041 gaaacagatc tgtcattaga gaaaataaat tttgattctc gaacagtaca attagagata
    80101 caaagatatc agcaaggcga tcccttttta aacccgactc aaacggaaca aaacagaaca
    80161 aacatggaac agccattaat ggaaccatca aatgaccgtt ttaacactca agtgatcttg
    80221 tggtctaaaa atggtgagat attgaataaa gcagctattg gtgggcgttt caaccaaatc
    80281 aaagaattaa aaatggatac gaaaaatctg aataccatcc aaaatattga attaaatgaa
    80341 gacagtggct catctgccct ctctttccgt tcgattacga aaaaagcgga gacgaatcaa
    80401 gatggcgttg cctacatcca aattttagaa aatacaaatc aaatcaagaa ttcattagcg
    80461 acttttcgca cgattatgat tttgtgtatg attgtttttt ggattctctc tattggtatc
    80521 agttattact tgtctagtct aagcatgcgc cctattattc tttcttggag aagacaaaaa
    80581 gaatttgttg agaatgcttc tcatgaattg cgcacaccgt taacgattat tcaaaatagt
    80641 ttggaacatc tttttaccaa gcctaatcat actattattg aagaatccga aagcatcgct
    80701 caagcattgt cagagacgag gcgcctcact ggattgactt cggatttgct gacaatcgct
    80761 cggaatgatt ctgatcagca gctgctttca aagcaaatga tcaatacaca agaatatatc
    80821 gaacaacttg ttaaaccatt tcaagagatg gccatcatag atggaaaaga gtttattctc
    80881 cataacgatg caacattaga agtaccaatt gatgaaaaaa aaatccatca agttttggtt
    80941 attttgctgg ataatgcatt aaaatatacg aatccagaag ataagatcat tttggattca
    81001 tctgttaacc ataaagaatg gctgattcac gtaaagaata ctggcccttc tatcagtgaa
    81061 gaagatcaag caaggatttt tgatcgattt tatcgggaag atcaatctcg ggcgaaagag
    81121 actggcggat acgggcttgg cttggcaatt gcaaaacagg taatcgaaca gcatcatgga
    81181 cagatttctg tatccaatta tcttccacaa ggagtcgatt ttaaagtcca attgccgttg
    81241 aagcctaaaa aataatgctt atccctaaaa aagggctgtg acaaaaatct tgtcacagcc
    81301 cttttttgtt gcgaataaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    81361 tctttaagct gaccacttct gtcacaacct ctttagctta cgcttacgat tttttattgc
    81421 ggtcttcaat gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt
    81481 ctgtcacttt ttcaacatct tcatgttctt ctttatgaat cccaacttct tcacgaataa
    81541 ctgtatgttt gcttacatct acttgctctt ctttgattgg gatagtaatc gtgtcatctt
    81601 ccaatttttc gtctgttccc tgtgaagatt gatctgttac tggtttgcgt tcgatgacga
    81661 tttcttcacg tttgaccggt acttctaccg tttcagtatc gttgaccaca tgtttgtgga
    81721 tgtcgacttc tccagttgtc acatcatgtg tatttacatc taatttttct tcttttagtt
    81781 gaattgtatc atcatctaga tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca
    81841 ttgtatcagt ctcgccaggt acgtccatcg gatcagttcc tggtgtcata ttcaatggat
    81901 ccgttgtatt ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt ccagtgccag
    81961 tttctaatcc agtccccatt gtgcttgctg ggctaacagt atcttgtgta tccattgtat
    82021 cttctgttgt ttctggctga cgatagcctt ttactgtgat gacaaatttt ccatcagaaa
    82081 tatctcctct atattgatat aaaggatcat tttctggtgt agccgtctca gaatcatagg
    82141 tatcaaaaga aaaagtatct tttatttttt cccataaaga acggtcttct tcaccatcca
    82201 tccgattttc atctgtttca acatctatac cagacaatcc gtcaaatcct agtctagatg
    82261 ctgcttcatc agtagtataa ataacgatat catctcgttc atacccttcg ttacgcaacc
    82321 gttcaattac tgataatgct tcttccctcg tactatagct tccgatgacc gttaaatttt
    82381 tctcttccat gtaaattcct cctagcaaat agatttatga ttggtata
//
LOCUS       DACEJR010000005        77072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEJR010000005 DACEJR010000000
VERSION     DACEJR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.005"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..445)
                     /locus_tag="IW651_000338"
     CDS             complement(161..445)
                     /locus_tag="IW651_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813098.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IW651_000339"
     CDS             complement(463..1353)
                     /locus_tag="IW651_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0813099.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IW651_000340"
     CDS             complement(1373..1591)
                     /locus_tag="IW651_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813100.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IW651_000341"
     CDS             complement(1561..3261)
                     /locus_tag="IW651_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0813101.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IW651_000342"
     CDS             3528..4301
                     /locus_tag="IW651_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813102.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW651_000343"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW651_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0813103.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IW651_000344"
     CDS             complement(5125..6096)
                     /locus_tag="IW651_000344"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0813104.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW651_000345"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW651_000345"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0813105.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW651_000346"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW651_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0813106.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IW651_000347"
     CDS             complement(9051..9875)
                     /locus_tag="IW651_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0813107.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IW651_000348"
     CDS             complement(9848..10528)
                     /locus_tag="IW651_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813108.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IW651_000349"
     CDS             complement(10551..11489)
                     /locus_tag="IW651_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813109.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IW651_000350"
     CDS             complement(11734..12585)
                     /locus_tag="IW651_000350"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0813110.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW651_000351"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW651_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0813111.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IW651_000352"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IW651_000352"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0813112.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQLATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW651_000353"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW651_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0813113.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW651_000354"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW651_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0813114.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IW651_000355"
     CDS             complement(19463..20704)
                     /locus_tag="IW651_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0813115.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW651_000356"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW651_000356"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0813116.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IW651_000357"
     CDS             complement(21731..22615)
                     /locus_tag="IW651_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813117.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23393..24679)
                     /locus_tag="IW651_000358"
     CDS             complement(23393..24679)
                     /locus_tag="IW651_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0813118.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24672..25940)
                     /locus_tag="IW651_000359"
     CDS             complement(24672..25940)
                     /locus_tag="IW651_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0813119.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26192..26653
                     /gene="mscL"
                     /locus_tag="IW651_000360"
     CDS             26192..26653
                     /gene="mscL"
                     /locus_tag="IW651_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0813120.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26712..27158)
                     /locus_tag="IW651_000361"
     CDS             complement(26712..27158)
                     /locus_tag="IW651_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813121.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27251..27496)
                     /locus_tag="IW651_000362"
     CDS             complement(27251..27496)
                     /locus_tag="IW651_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813122.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27611..28189)
                     /locus_tag="IW651_000363"
     CDS             complement(27611..28189)
                     /locus_tag="IW651_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0813123.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28266..28796)
                     /locus_tag="IW651_000364"
     CDS             complement(28266..28796)
                     /locus_tag="IW651_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0813124.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28800..29660)
                     /gene="rlmB"
                     /locus_tag="IW651_000365"
     CDS             complement(28800..29660)
                     /gene="rlmB"
                     /locus_tag="IW651_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0813125.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29653..30048)
                     /locus_tag="IW651_000366"
     CDS             complement(29653..30048)
                     /locus_tag="IW651_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0813126.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30045..31457)
                     /locus_tag="IW651_000367"
     CDS             complement(30045..31457)
                     /locus_tag="IW651_000367"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0813127.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31454..31990)
                     /gene="cysE"
                     /locus_tag="IW651_000368"
     CDS             complement(31454..31990)
                     /gene="cysE"
                     /locus_tag="IW651_000368"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0813128.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32312..33772)
                     /locus_tag="IW651_000369"
     CDS             complement(32312..33772)
                     /locus_tag="IW651_000369"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0813129.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33942..35099)
                     /locus_tag="IW651_000370"
     CDS             complement(33942..35099)
                     /locus_tag="IW651_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0813130.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35133..36503)
                     /gene="radA"
                     /locus_tag="IW651_000371"
     CDS             complement(35133..36503)
                     /gene="radA"
                     /locus_tag="IW651_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0813131.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36566..37093)
                     /locus_tag="IW651_000372"
     CDS             complement(36566..37093)
                     /locus_tag="IW651_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0813132.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37323..38129
                     /gene="proB"
                     /locus_tag="IW651_000373"
     CDS             37323..38129
                     /gene="proB"
                     /locus_tag="IW651_000373"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0813133.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38126..39373
                     /locus_tag="IW651_000374"
     CDS             38126..39373
                     /locus_tag="IW651_000374"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0813134.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39449..39793
                     /locus_tag="IW651_000375"
     CDS             39449..39793
                     /locus_tag="IW651_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813135.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39910..40236)
                     /locus_tag="IW651_000376"
     CDS             complement(39910..40236)
                     /locus_tag="IW651_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813136.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40609..41810)
                     /locus_tag="IW651_000377"
                     /pseudo
     CDS             complement(40609..41810)
                     /locus_tag="IW651_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41927..42442
                     /locus_tag="IW651_000378"
     CDS             41927..42442
                     /locus_tag="IW651_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0813137.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42641..43087)
                     /locus_tag="IW651_000379"
     CDS             complement(42641..43087)
                     /locus_tag="IW651_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0813138.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43134..44612)
                     /gene="msr"
                     /locus_tag="IW651_000380"
     CDS             complement(43134..44612)
                     /gene="msr"
                     /locus_tag="IW651_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0813139.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44966..45595)
                     /locus_tag="IW651_000381"
     CDS             complement(44966..45595)
                     /locus_tag="IW651_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0813140.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45579..46043)
                     /locus_tag="IW651_000382"
     CDS             complement(45579..46043)
                     /locus_tag="IW651_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0813141.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46530..47798)
                     /locus_tag="IW651_000383"
     CDS             complement(46530..47798)
                     /locus_tag="IW651_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0813142.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48029..48766)
                     /locus_tag="IW651_000384"
     CDS             complement(48029..48766)
                     /locus_tag="IW651_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0813143.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48833..49402)
                     /locus_tag="IW651_000385"
     CDS             complement(48833..49402)
                     /locus_tag="IW651_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0813144.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49530..50402
                     /locus_tag="IW651_000386"
     CDS             49530..50402
                     /locus_tag="IW651_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0813145.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50744..51616
                     /locus_tag="IW651_000387"
     CDS             50744..51616
                     /locus_tag="IW651_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0813146.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51613..53571
                     /locus_tag="IW651_000388"
     CDS             51613..53571
                     /locus_tag="IW651_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0813147.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53822..54673)
                     /locus_tag="IW651_000389"
     CDS             complement(53822..54673)
                     /locus_tag="IW651_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0813148.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54796..55263
                     /locus_tag="IW651_000390"
     CDS             54796..55263
                     /locus_tag="IW651_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0813149.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55526..56008)
                     /locus_tag="IW651_000391"
     CDS             complement(55526..56008)
                     /locus_tag="IW651_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0813150.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56108..56971)
                     /locus_tag="IW651_000392"
     CDS             complement(56108..56971)
                     /locus_tag="IW651_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0813151.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57569..58417
                     /locus_tag="IW651_000393"
     CDS             57569..58417
                     /locus_tag="IW651_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813152.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58588..58929)
                     /locus_tag="IW651_000394"
     CDS             complement(58588..58929)
                     /locus_tag="IW651_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0813153.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59015..59722
                     /locus_tag="IW651_000395"
     CDS             59015..59722
                     /locus_tag="IW651_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0813154.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59709..60032
                     /locus_tag="IW651_000396"
     CDS             59709..60032
                     /locus_tag="IW651_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0813155.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60121..61158)
                     /locus_tag="IW651_000397"
     CDS             complement(60121..61158)
                     /locus_tag="IW651_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0813156.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61605..62588)
                     /locus_tag="IW651_000398"
     CDS             complement(61605..62588)
                     /locus_tag="IW651_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0813157.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63004..63774)
                     /locus_tag="IW651_000399"
     CDS             complement(63004..63774)
                     /locus_tag="IW651_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813158.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63767..64702)
                     /locus_tag="IW651_000400"
     CDS             complement(63767..64702)
                     /locus_tag="IW651_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813159.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65296..66945)
                     /locus_tag="IW651_000401"
     CDS             complement(65296..66945)
                     /locus_tag="IW651_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0813160.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67140..68036)
                     /locus_tag="IW651_000402"
     CDS             complement(67140..68036)
                     /locus_tag="IW651_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0813161.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68029..68709)
                     /locus_tag="IW651_000403"
     CDS             complement(68029..68709)
                     /locus_tag="IW651_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0813162.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68710..69492)
                     /locus_tag="IW651_000404"
     CDS             complement(68710..69492)
                     /locus_tag="IW651_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0813163.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69485..70402)
                     /locus_tag="IW651_000405"
     CDS             complement(69485..70402)
                     /locus_tag="IW651_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0813164.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70405..70689)
                     /locus_tag="IW651_000406"
     CDS             complement(70405..70689)
                     /locus_tag="IW651_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0813165.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            71477..74704
                     /gene="fss3"
                     /locus_tag="IW651_000407"
     CDS             71477..74704
                     /gene="fss3"
                     /locus_tag="IW651_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0813166.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            74817..75131
                     /locus_tag="IW651_000408"
     CDS             74817..75131
                     /locus_tag="IW651_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0813167.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            75144..75518
                     /locus_tag="IW651_000409"
     CDS             75144..75518
                     /locus_tag="IW651_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0813168.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            75528..75863
                     /locus_tag="IW651_000410"
     CDS             75528..75863
                     /locus_tag="IW651_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0813169.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            75946..>77072
                     /locus_tag="IW651_000411"
     CDS             75946..>77072
                     /locus_tag="IW651_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0813170.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        22409 a        16232 c        13891 g        24540 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gctagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
    23401 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
    23461 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
    23521 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
    23581 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
    23641 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
    23701 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
    23761 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
    23821 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
    23881 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
    23941 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
    24001 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
    24061 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
    24121 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
    24181 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
    24241 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
    24301 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
    24361 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
    24421 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
    24481 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
    24541 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
    24601 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
    24661 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
    24721 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
    24781 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
    24841 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
    24901 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
    24961 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
    25021 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
    25081 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
    25141 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
    25201 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
    25261 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
    25321 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
    25381 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
    25441 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
    25501 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
    25561 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
    25621 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
    25681 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
    25741 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
    25801 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
    25861 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
    25921 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
    25981 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
    26041 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
    26101 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
    26161 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
    26221 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
    26281 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    26341 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    26401 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    26461 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    26521 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    26581 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    26641 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    26701 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    26761 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    26821 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    26881 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    26941 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    27001 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    27061 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    27121 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    27181 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    27241 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
    27301 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
    27361 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
    27421 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
    27481 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
    27541 ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga
    27601 tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc
    27661 tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa
    27721 aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt
    27781 cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt tcttgcaatg
    27841 tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat
    27901 ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc
    27961 ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca
    28021 atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta
    28081 aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa
    28141 atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc
    28201 tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg
    28261 atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc
    28321 tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg
    28381 gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat
    28441 gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt
    28501 gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct
    28561 tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact
    28621 agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa
    28681 cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt
    28741 tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc
    28801 tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca
    28861 tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc
    28921 aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca
    28981 ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct
    29041 ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact
    29101 gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg
    29161 atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga
    29221 tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca
    29281 atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc
    29341 acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct
    29401 tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga
    29461 aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg
    29521 aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc
    29581 tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg
    29641 tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc
    29701 ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt
    29761 tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct
    29821 tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg
    29881 aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt
    29941 gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc
    30001 tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc
    30061 catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga
    30121 atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat
    30181 tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc
    30241 cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct
    30301 agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta
    30361 aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta
    30421 tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac
    30481 gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg
    30541 atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt
    30601 ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct
    30661 tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc
    30721 gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca
    30781 tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa
    30841 ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc
    30901 cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt
    30961 gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta
    31021 cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact
    31081 tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggc
    31141 tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct
    31201 ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa
    31261 ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa
    31321 gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca
    31381 tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta
    31441 ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg
    31501 ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac
    31561 cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca
    31621 tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg
    31681 ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc
    31741 ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac
    31801 cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat
    31861 ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga
    31921 tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa
    31981 accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat
    32041 aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc
    32101 cacttttctt cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc
    32161 tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca
    32221 gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga
    32281 tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta
    32341 atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc
    32401 ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt
    32461 gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct
    32521 gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg
    32581 cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag
    32641 catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt
    32701 catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa
    32761 atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat
    32821 cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt
    32881 gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca
    32941 ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt
    33001 cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg
    33061 ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat
    33121 ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa
    33181 aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg atattatcgg
    33241 attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca
    33301 cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg
    33361 tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc
    33421 gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca
    33481 gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt
    33541 ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact
    33601 ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa
    33661 agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa
    33721 tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc
    33781 tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa
    33841 tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc
    33901 caactggttg aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc
    33961 tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag
    34021 atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc
    34081 atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct
    34141 tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg
    34201 actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca
    34261 gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat
    34321 ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca
    34381 gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca
    34441 cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg
    34501 atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg
    34561 atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac
    34621 agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa
    34681 actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg
    34741 atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg
    34801 gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga
    34861 taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt
    34921 ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat
    34981 gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat
    35041 gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc
    35101 gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca
    35161 gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg
    35221 tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca
    35281 atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt
    35341 ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc
    35401 gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga
    35461 agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga
    35521 cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct
    35581 accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct
    35641 aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc
    35701 atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc
    35761 cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac
    35821 attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc
    35881 ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg gctgacactt
    35941 cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa
    36001 tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga
    36061 taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt
    36121 tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt
    36181 aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc
    36241 accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct
    36301 tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc
    36361 cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg
    36421 caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat
    36481 tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa
    36541 atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt
    36601 gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac
    36661 gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat
    36721 ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca
    36781 acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt
    36841 ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt
    36901 ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt
    36961 tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat
    37021 gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc
    37081 ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac atatccgagg
    37141 ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa gtaaaccatt
    37201 aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt tacatgactt
    37261 tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga ttgaggtgtc
    37321 acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga acaagtacat
    37381 tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct tttgtccttt
    37441 ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct gtaggcgttg
    37501 gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag caagctgcgg
    37561 cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt gtatataatc
    37621 aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa agtcgtaaaa
    37681 atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc gtgaacgaaa
    37741 acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat aatgatcagc
    37801 tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt tctgatatcg
    37861 acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat cgctatatcg
    37921 ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca tttggaacag
    37981 gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac agcgcaatga
    38041 ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga aaagaagtag
    38101 gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac aacaggcaaa
    38161 agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc tacttgttca
    38221 aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc ataaagactt
    38281 agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc agctgacacc
    38341 tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag acgatcctat
    38401 tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca aaaagcgcgt
    38461 gccactaggc gtgaccggca tcatttacga atctcgtcca aatgtcacga cagatgcagc
    38521 tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag aagcattctt
    38581 ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg gcgaatctcc
    38641 ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt tgatgcgatt
    38701 gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc aacgagtcaa
    38761 agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg tatatatcga
    38821 taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag cttcccgtcc
    38881 atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac cttttttctt
    38941 gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg atgctcgtgc
    39001 gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata ctgaatatct
    39061 agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg cacatatcaa
    39121 tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg ctagtcaacg
    39181 tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc gttttacaga
    39241 cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt tgcatgcccg
    39301 tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg gcgatggaca
    39361 aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt tttctgtaaa
    39421 ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt tttttattta
    39481 ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc ttttctttca
    39541 attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt ttgacaaaat
    39601 cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat tatgcttatt
    39661 agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga ttacgctact
    39721 gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc acttgaatcg
    39781 gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc ggcaaaaaac
    39841 tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt ccatataaac
    39901 ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa gcgaaaatcc
    39961 gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt tcatcgtttt
    40021 ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct gatacatacc
    40081 agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta ttggcaattc
    40141 tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta attcaacgag
    40201 tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca ttctttttct
    40261 taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg agaaaaaaag
    40321 ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt tcttctgcca
    40381 actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt tgaaattgtt
    40441 ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc gttcaaaagc
    40501 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc
    40561 tgttcttatg acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc
    40621 atttgtagca aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc
    40681 acctgcaaaa taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa
    40741 tggtgctaaa gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg
    40801 ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa
    40861 aaatcctgct aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa
    40921 taacagcaaa caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa
    40981 cttccgctca agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc
    41041 aaaaaataca aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc
    41101 aagtaaagta aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt
    41161 atgattgctt aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt
    41221 ttcaggtaaa agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa
    41281 aaatggataa cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat
    41341 tccgcagcca agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat
    41401 actgacgatc gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc
    41461 acgtaaccca atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac
    41521 gatgataaac aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct
    41581 gtaaatagca tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca
    41641 gctggcgtag cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact
    41701 cccataaaag cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt
    41761 tcttcatttg tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct
    41821 ctattagcat catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac
    41881 tttactgtga tataataaag aaaataagac gagaggtagg atttttatga atactttagc
    41941 ctatatatta ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct
    42001 gatcacaatt ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga
    42061 ccagcagggt tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca
    42121 cttgactgat gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc
    42181 tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac
    42241 agctactgga ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca
    42301 aaaaaaattg aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat
    42361 ccagcgcaga attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca
    42421 aatgcagcag ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga
    42481 gcaaattatc ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca
    42541 ggccaaaacc tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca
    42601 aaaagcatgc caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg
    42661 cttttagcac acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa
    42721 tcacattcat tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt
    42781 ccatgatcac accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa
    42841 tagttaagat catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt
    42901 tcgccagcac accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta
    42961 gaccaatcaa caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca
    43021 tccccaagac catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg
    43081 taggcaatct gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa
    43141 tttctcgtaa gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta
    43201 cgctcgacaa agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt
    43261 agcttctcta aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc
    43321 aacacattac ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca
    43381 cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa
    43441 ctacggacaa gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct
    43501 tcagatgtca tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga
    43561 aggattcctt ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt
    43621 tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat
    43681 gttctcccac ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt
    43741 tcaagagatt ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct
    43801 cctatttttt ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca
    43861 ctgtcttttt gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta
    43921 ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat
    43981 cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc
    44041 ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact
    44101 tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc
    44161 gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg
    44221 gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg
    44281 atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg
    44341 aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc
    44401 aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct
    44461 attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct
    44521 tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt
    44581 agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa
    44641 caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca
    44701 tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca
    44761 cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac
    44821 atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca
    44881 gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag
    44941 ctactgaata gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac
    45001 gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat
    45061 aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg
    45121 gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag
    45181 aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga
    45241 tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt
    45301 tatagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag
    45361 aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt
    45421 cctcgctttc agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg
    45481 tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata
    45541 cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta
    45601 ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt
    45661 gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct
    45721 ttatggtcca tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac
    45781 acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc
    45841 ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt
    45901 agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg
    45961 atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg
    46021 tgactaatct cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt
    46081 gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa
    46141 aaacgctctc aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa
    46201 tgataaagaa agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat
    46261 agctcttctg cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag
    46321 aatcccgtcg aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga
    46381 caagatgttg tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa
    46441 taagttcaac caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg
    46501 atgcagggca gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat
    46561 ctccagccca tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta
    46621 ccagtttttt catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa
    46681 cttagacctt cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt
    46741 gtgtaatgat ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt
    46801 ggtgcttgcc aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct
    46861 ccggcattca atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga
    46921 gaagttgaag ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc
    46981 aatgtagaac cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct
    47041 ttgtaagagt tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa
    47101 gaagtcgtct tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt
    47161 ctaaaagcgg cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta
    47221 acagattggc cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc
    47281 aaagtactgt tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat
    47341 aagtcattgg cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt
    47401 tgagaattag cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac
    47461 aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct
    47521 tctgcattct ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt
    47581 tctgttgttt cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac
    47641 tgcaaagtac tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt
    47701 gcgacagttt ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct
    47761 aaaagaataa cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg
    47821 aaacttttcc taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta
    47881 aataacattt gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag
    47941 aatattcttc tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta
    48001 attctctttg gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca
    48061 tgttactgac agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat
    48121 cttcggaggt caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt
    48181 cgctgatggt ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga
    48241 tgttattttg ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg
    48301 ttccgcaata aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa
    48361 tgatatgccc agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca
    48421 cacccatgat gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg
    48481 gagcggttgg catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca
    48541 tcgctaaatc gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat
    48601 atagctcagc ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag
    48661 ctataactag tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc
    48721 ttgaagcgcc catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct
    48781 gttttgaaaa atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa
    48841 tccgttcgac aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga
    48901 catctttcgt aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg
    48961 gcagaatagt tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg
    49021 acaagcctct tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag
    49081 gaccaatcaa tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac
    49141 gattgataaa gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca
    49201 gcagaacagt caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga
    49261 tatctgctgg ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt
    49321 ggatctgatg gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt
    49381 gtttttcgat gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg
    49441 cttatcatgt ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg
    49501 tatgcttttt taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag
    49561 cagtagctag agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta
    49621 ctctctcaaa acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcggg
    49681 gctcccgaga gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa
    49741 tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag
    49801 aaatctacat tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt
    49861 cgttgatcaa ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata
    49921 tcatagacaa actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca
    49981 aacaaaaata ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc
    50041 gaaatcatcc tttagcaaag caaaccgtca tccatccgca ggaactgaaa actgttcctt
    50101 tgatcatttc ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca
    50161 tagatcagtt atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg
    50221 ataacgggac ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa
    50281 aaatattcat cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag
    50341 accagcctct ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt
    50401 aagtagagtt tttttccttc gctttcagct ttttaacaac gaataagata agcaaaagat
    50461 ccaacgatta aataacataa gtttcttaat ttatttcttc cttttatttc tgataattcc
    50521 agtaaattac tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg
    50581 attacgttaa caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg
    50641 aggaacatgg tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg
    50701 gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat
    50761 ctttaatcaa aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa
    50821 agggattgga tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat
    50881 atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga
    50941 acgcttttta tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa
    51001 gttgaatgaa catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat
    51061 taataacggt atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga
    51121 tgaattcagt attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata
    51181 tacttatgac gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa
    51241 caataaccat cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga
    51301 atcagaattg gaaacagata tccattcaga accattttca ctgaattatt caagactggt
    51361 gaatcattta cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac
    51421 ggaaatcgta gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat
    51481 ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt
    51541 ggccatccat atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga
    51601 ggaggaaaaa aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt
    51661 gcggtcttac ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt
    51721 aacgacattg tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct
    51781 ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc
    51841 ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct
    51901 actttattga atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta
    51961 tttattggta ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca
    52021 aaattaccga tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc
    52081 tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct
    52141 ttagtttctt ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc
    52201 ttcttcaacc gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg
    52261 tttgacgtag caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc
    52321 gtaacaggaa tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga
    52381 gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg
    52441 ctagcagcag cttttgcttc attctttact ggtgtgacag aaccacttga attttcattt
    52501 atgttcgttg cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca
    52561 gtgagtgcat tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac
    52621 ttcctatctt tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta
    52681 gtaacagcag tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg
    52741 actcctggcc gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac
    52801 aataaatatg cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta
    52861 acttcgatcg ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc
    52921 gatcaaaata aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat
    52981 gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat
    53041 ggtggtgctg cagcacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc
    53101 ggtgaaacga tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact
    53161 gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca
    53221 gaaaatggag aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa
    53281 catgctgtcg gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac
    53341 actgttgact taaaaggcga accattcact ttatatgtag aagaaggtca aaaagtcgca
    53401 agaggtcaat tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg
    53461 gatatggtcg ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca
    53521 cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc
    53581 ttccccagac ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct
    53641 cctttttccg aataaaaacg gcgttctcct gccaactccg cggtaataag tccagctaaa
    53701 cgaaacatat gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct
    53761 ttttcacaaa atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac
    53821 attattcctg atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca
    53881 atactttttc taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga
    53941 tcgctaaaaa catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa
    54001 acgctgcatc acttgcttca ataatttcta actctttacc tgtcgcacgc tctaatgcct
    54061 tggctaattc ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac
    54121 tttctactag tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt
    54181 ttccatttcc tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta
    54241 attcattctc tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa
    54301 tcattttttc agtgaattgg tgatctggcg ctaatatact gatcgagtga tcggcatctg
    54361 caaaacttgt ataagcgata taagataccc cagctgcttg tgctgccttc actacattct
    54421 tatgttcggc ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt
    54481 tcaacgcttc tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag
    54541 cttctttcag tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg
    54601 gaaccatctt ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta
    54661 ctaaataatg catatttgcc actccctatt ctttttattt gttaagattt acagctgtac
    54721 atttataaat tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg
    54781 aaaggatgac aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc
    54841 tatattgaaa tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc
    54901 gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc
    54961 tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta
    55021 ctagatgtct atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca
    55081 aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg
    55141 ctgcagaaaa aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa
    55201 atagccgttt tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta
    55261 taattctctc tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat
    55321 tctccggggg acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc
    55381 gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct
    55441 gaaaaatgca aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag
    55501 cgctactttc acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc
    55561 ccatatattt cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg
    55621 cgtaatcttc tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta
    55681 attcttcagg cgattctgct aaagcgctta caacaagata cgttcctttt gggaatggga
    55741 tgattcgcgt agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt
    55801 tcccgttata gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg
    55861 catctattgt tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac
    55921 cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg
    55981 gtttttcttc gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat
    56041 cttacataaa attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat
    56101 tttttaacta tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat
    56161 cctctcccca ttttgtaaaa gagatctcgg gagcttcgac taacccataa tctgaagaag
    56221 gaagccattc tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt
    56281 tattaggaaa aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg
    56341 gattttcttt tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc
    56401 ggtctccatc aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact
    56461 tatgcatctc ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat
    56521 tgactccttc aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt
    56581 tcttttcgat acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc
    56641 gtggaaaaga tttttgtatg ccgtatctac tgatttctga aggcagatat cctgaccaat
    56701 cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg
    56761 tcactcttga tccatctaga agatcgcgat tagccaaagc caatctacgg tttttgatat
    56821 agtccattag cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga
    56881 ttcctgtatt ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt
    56941 aatcgatcaa ttggttcaga tctttaagca tatttatctt cctccttgat tgccttttta
    57001 tcatattatc aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag
    57061 aaaatgttgt atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta
    57121 ttatctgcct atctatgttt tacctttaga aaaataaatt ttggggaaga cagattctaa
    57181 gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt
    57241 acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat
    57301 acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt
    57361 ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt
    57421 tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt
    57481 gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata
    57541 aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa
    57601 cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac
    57661 aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg
    57721 ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa
    57781 acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc
    57841 actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt
    57901 aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta agatgccatt
    57961 tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg atcatattat
    58021 caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca
    58081 agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa
    58141 tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct
    58201 tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga
    58261 aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc
    58321 aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca
    58381 aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa
    58441 tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta
    58501 aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc
    58561 atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg
    58621 atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg
    58681 ttccgctgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca
    58741 ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct ccacacttaa
    58801 acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa gatttttcgc
    58861 gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt tacgtttcaa
    58921 tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta acgacaatta
    58981 tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac
    59041 tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac ttgcatttgg
    59101 tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt ccctgctggt
    59161 ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca gcccattgct
    59221 ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat
    59281 tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac
    59341 agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt
    59401 tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt
    59461 tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat
    59521 cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa
    59581 acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt
    59641 tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat tgggggtgtg
    59701 gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa
    59761 cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta ccgcaaccgt
    59821 tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc
    59881 tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag
    59941 ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggcaggaa
    60001 ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt
    60061 actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc
    60121 ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg tttctacgtc
    60181 tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt
    60241 actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt
    60301 ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga
    60361 ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg
    60421 tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat cgaataatac
    60481 agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg
    60541 ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc
    60601 agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc
    60661 cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt ctggagttag
    60721 accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcagc
    60781 ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ctagttggta
    60841 ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat cgccgacaga
    60901 aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa aacctaagat
    60961 acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga
    61021 tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc
    61081 atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga ctaccttcat
    61141 taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag catccataca
    61201 gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc atcataatta
    61261 ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga
    61321 cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc acaagtcaaa
    61381 atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttaaac
    61441 ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg gtattagctc
    61501 aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac ttgatacagg
    61561 acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg ttcactgaaa
    61621 agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc agtagatttc
    61681 gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat atagatttta
    61741 aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg atcttgcaga
    61801 tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct cgggtctttt
    61861 tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc aacgccatat
    61921 ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat cgtgattagt
    61981 gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc ccaatgtgca
    62041 tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc tatatcttga
    62101 tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc gatacgcgtc
    62161 ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc cgctacttct
    62221 ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac tgggcagccc
    62281 atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat ttctctaaaa
    62341 aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc tgtaaatgtt
    62401 aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga gtttgtaaac
    62461 tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa tacaacatct
    62521 gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt ccaaaaatta
    62581 gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat ctttctcaaa
    62641 aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt aaaagatgtt
    62701 tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt ttccggaata
    62761 aatggtgttc aaaagcttgc atttgcggta ataagtccaa ccaaacgaaa aataggagga
    62821 gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca caacctctcc
    62881 actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac aaaatatgag
    62941 gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
    63001 tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg cgtacaaaac
    63061 aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga tcgttccaag
    63121 gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg aaacagcttg
    63181 ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag ttaacggctg
    63241 caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa tcccgttgag
    63301 cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga ccatgaacca
    63361 tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag agccgacgag
    63421 aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg taggaacagg
    63481 tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg acgaagaaac
    63541 attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga tgaattccat
    63601 atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga aaataacaaa
    63661 atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac gcaaatagcg
    63721 gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa acatttttct
    63781 cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa cgattcgact
    63841 tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag attccgttca
    63901 ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat gtacaagcat
    63961 atccttcaat cacaggtttg gtatcatatg gcgctagatc aaagataaat gtttcaaatt
    64021 gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc gattggatga
    64081 ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc aaaatgatcg
    64141 ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc aactcgatgt
    64201 caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc aatgccctag
    64261 caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca tttctctttt
    64321 cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct ttgcgagaga
    64381 caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa gggttaaaat
    64441 tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc ttgtccagat
    64501 cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta ataatcccga
    64561 tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct ccttctttca
    64621 ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt ttcaaattat
    64681 ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat tcttaattgt
    64741 atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca cacctgctaa
    64801 caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact atctaaacaa
    64861 ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga catatagaag
    64921 atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc cgtcctttta
    64981 tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag ttattttcaa
    65041 tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg tctcgtcgct
    65101 gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt attttgtatc
    65161 gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat tgaattctgc
    65221 cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc acgcatcatg
    65281 cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga acaacaaact
    65341 ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca gcaaggcaat
    65401 cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga ttccttttcc
    65461 ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa tctctgtcaa
    65521 ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc accgaacgac
    65581 tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct catctacgaa
    65641 gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg atttgatttc
    65701 tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg aagtcaacac
    65761 atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct gtgctagaat
    65821 acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg aacccgtgac
    65881 taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca atggatgata
    65941 gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga tcggcatata
    66001 ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt cccacagctt
    66061 gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg agataaatgg
    66121 cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc cgctcttcat
    66181 tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct gtttcagctc
    66241 tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg ttgaaaccaa
    66301 ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat aatagaccgt
    66361 attgaatact aagccaccga ttgctagata gattcctagt acttgcccta aaagaagcaa
    66421 acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc gtccaatctt
    66481 tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt ttcctaaacg
    66541 agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa aagcaattag
    66601 tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat acaacgcttc
    66661 tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca tatccaagtc
    66721 ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct ccgtgagcca
    66781 cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt ttccccaatt
    66841 actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa ctaataaaat
    66901 aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc tctccgttcc
    66961 ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt acaaaaataa
    67021 ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt ccaaaaaaga
    67081 agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat tagagccaat
    67141 taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct agcggtcatc
    67201 tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc gctagattcc
    67261 gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc aacagaggtt
    67321 tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc ctcttttgaa
    67381 aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat attttgcagc
    67441 actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg ttcagcgaac
    67501 gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac ttcttccacc
    67561 tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc tattcgagca
    67621 agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt gatgatcgtt
    67681 aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac ataaccgtta
    67741 atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag tctgatgttt
    67801 tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca tgtattcagt
    67861 gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg accaccatat
    67921 tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc cctttgcagg
    67981 aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt aatcaaatct
    68041 aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta gtttccctcg
    68101 aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt cttgccaaac
    68161 tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa aaagcttcag
    68221 cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta catccctcga
    68281 ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt ttacttggac
    68341 agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga tatcaaatgg
    68401 tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc tttgatcgct
    68461 ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg ctaacaatgc
    68521 ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag gatatgtgct
    68581 taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc tctgttcaag
    68641 aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa tagtttccag
    68701 caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag ctgatgatac
    68761 cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag ttgcttgttg
    68821 cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa attccaagcc
    68881 tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa gaaaagaaag
    68941 aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa cagataatgt
    69001 cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc catagttttt
    69061 gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg ctagccccgt
    69121 aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg acacataata
    69181 agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt gaaattcttc
    69241 cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag ccattttttc
    69301 caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt ttaattcatt
    69361 gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa gtacgaccat
    69421 cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt cagcagttaa
    69481 tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc agtttttgcc
    69541 caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga ttgatggctg
    69601 ctacttggtc aattccttca aaaagctgga taacacggct agattggaca aaataatccc
    69661 tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg tcaattttta
    69721 taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc aagtgtcctt
    69781 catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag tggctagaaa
    69841 gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc atttctgcca
    69901 tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct ggattcgtga
    69961 tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc tttgctttct
    70021 ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct ctatccgaca
    70081 caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct ggataaagag
    70141 caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc gtcgttccaa
    70201 ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc ataaaggtag
    70261 acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg atttcgatcg
    70321 atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt gctttcaaga
    70381 ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg aagtatagtt
    70441 gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt ccaattgaat
    70501 ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat caaagagcgc
    70561 accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta tcgttgacat
    70621 cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa
    70681 agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt ttgggattta
    70741 caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct tcttccgaat
    70801 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca aacatgagaa
    70861 gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaga
    70921 agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt tatagatcga
    70981 atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa aaaaggcact
    71041 ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct cagcattgct
    71101 tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga
    71161 gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat cattgaataa
    71221 gtttgaaaaa ccttgattta taaaggtttt cccgtaaata tattttatta tatttgatta
    71281 aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata tttcgtattt
    71341 tagattaaat ttttaggctc attcattctc aaatccattc tattcagtca ataaaaattt
    71401 taaaaagact ttataaacaa agatgttcaa gcgagcatct tatttttttg tgcttaattt
    71461 gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt
    71521 gagtttggct gttcaagcaa ttgttggagc aattgctcct acgattactt ttgctgatga
    71581 aattacccat ccacaaacgg taaccgtaga actagattta gcacatcaat atgctgttga
    71641 aggaactttt agtgatggtc gtccaatgtc agaagtcact gttccgcatt atgcagtgta
    71701 caacggcttt aaacaagata ttttctgtat tgaaccagga gtcccgattt acaatgagtt
    71761 cactcctggc tatgagaaaa acccgttacc agatatgtcc gataaagcca aattagtttc
    71821 cgttttatgg aaaaaagtag gtacagatgc agatactcat attgtggcac agaagatgat
    71881 ctggcaagaa gttaatgggt atacccttca ctcaataaaa cgatcagatg gtagtgccgt
    71941 aaatatcgca gccattgagg caaaaataaa tcaagccatt gacgattatc agaagaaacc
    72001 aaccttccat aatagtacgg ctaaaacagt attagggcaa tcgactactg tgacggatac
    72061 gaataatctg aatttgtcag agttcgatga ggttgtggaa aatgccgcaa acattgatta
    72121 tcgtgtgaat ggtaatcagt tgataatcac cccaaatgcc aattcaaaag aaagtggtgt
    72181 gctgacgctt aagaaatctg ctggtaccgg aacacctgtc gcctataaaa aggctggcca
    72241 acagacttta atggccgggg cgattgataa acctaatacc tacacaatca aaattgatgt
    72301 ggaaacggag ggttctttaa agatcaaaaa agtcgataaa gaatcgggtg atgttgtacc
    72361 aggaacggtt ttccatttag actttggaaa aactttacct gcaaaagacg tgactactga
    72421 caaagaaggc attgctacat tggatgagat tcctcatggt acaaaagtaa ccattaccga
    72481 aaaatcagta ccggcgcctt atacgattga taccaccccc atgactacca ccattaaagc
    72541 aggcgaaacc atttacgtga cttcaaaaaa cgcacgggaa aaagggcaaa tcattctgga
    72601 taaaagcgga gttgaaacag ggagtgatct ttggaatgac aattattcct tagctggtaa
    72661 tacgtttgcc attcgcaaag acagtccaac cggtgaaatc gttcaagaaa tgaccacgga
    72721 tgaaaacggt catgcggaaa caccaaaaga gattgctaat gcgttggaat tgggaaccta
    72781 ttatgtgacc gaaactaaag ctagtcatgg gttcgtgaat acgttcaaac ctgtaaaagt
    72841 cgaattgaag tatgccaatc aaaccgtagc ccttgttacc agtaacgtaa aagggcaaaa
    72901 tcaagaagtc actggcgaaa ccactttgac gaaagaagac aaagatactg gggataaagc
    72961 acaaggtaag gcagtctttg agggtgctga atacaccttg tttactgcta aggatggaaa
    73021 agctgttaaa tggagtgagg cttttaaacc agagttagcg aagggaacga aaggttctga
    73081 tgaaacggtc actttgactt tagacgaaaa gaaccaagtt gccgttaaac atttagctat
    73141 caacgagtat tattggcaag aaacaaaagc gcccgaagga tatagccttg atgaaacaaa
    73201 gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa aatgccgtga ttacccgaaa
    73261 tgttacggca aaagaacaag tgattcgctt tggctttgat ttctttaaat ttgctggatc
    73321 agctgcgggc actaccgaaa ctggcttcaa tgatctgacc tttaaagtgt cgccattgga
    73381 agggaccaat gaaatcacag gtgccgaaga tgaagcgacc acagcttata acgagcaatt
    73441 agggtttgat ggttatggca agtttgaaaa tcttccttat ggggattatt tacttgaaga
    73501 agtagaagcc ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt ctacttttaa
    73561 ggaaaacaaa gaagactttg tgaagagtga gtatgtcttt accattaccg aacaagacca
    73621 aaaacaacca attaaaacgg tgaccgttcc ttacgagaag ctgacgaaca aagcattttc
    73681 tgttagtttg aaccgtttaa tgctctatga tttgcctgag gaagaagata gtttaacttc
    73741 tcttgcgact tggaaagacg gaaacaaaga attaacttct ctggattcta ccgagctagt
    73801 tgataaattg agttacaatc tacatgaaat caaagaagac tggtatgtgg tagcgcaagc
    73861 cattgatgtg gatgcgacaa aagctgccca agaaaaagat gaaaaagcca aaccagtggt
    73921 gattgcagaa acaagcgcaa cgttggcgaa caaagagaaa acaggaactt ggaaaattca
    73981 gcataaatta accgccgaac aagttttaaa taaaactatt gtcttattca actatgtgta
    74041 tgaaaataag gaagcttttg aagcaggcga taagccagta gcgaaggatg ttagcttgaa
    74101 taaccaagcc caaactgtca gttgtacagt ggaacaccat gtttctatcc aaacaaaggc
    74161 ccacctagaa aacggttctc agacctttac tcatggtgat gtggtggata tgtttgatga
    74221 tgtgtcgatt actcatgatg tactggatgg ctcaaaagaa gctttcgaaa caattctgta
    74281 tgctttatta ccggatggta caaacaaaga aatttggaaa tctggcaaga ttgattatga
    74341 agtaaatgat aaagaattca ccaaaactgt gcttgcgaaa aaagtagata ccggaaaata
    74401 tccagaagga accaagttta cttttgcgga aatcaattac gataaagatg ggaatatcaa
    74461 tggaaaacac aatgaagatt tgaaagaaaa atctcaaacc ttaacaccaa aagaagttcc
    74521 aaccatacta agtacgccga aacaaccgga aacaccaact gttccaagtg gctctcaaga
    74581 atctagtccc acagtgaaga ccttccctca aaccggggag aaaaattcca atgttttact
    74641 gttcattggt tttactttga tttttgcgac ggcgggctat tatttctgga atcgccggaa
    74701 ctaaggtgat gagatgatac aagtaaatcc taagaaccaa cgtgcgcgac cgcctcctaa
    74761 aaaagtagtc gcgctatttt tgtatccaaa actaacaatg aaaagaggaa aagaagatgg
    74821 aattgaaatt tgtcgtgcca gatatggctg aaacctttgg aaagttaagt tatgccggtg
    74881 aaggagaaat tataacagaa ggatatggac ggaatactac ggtgattggt cgcagttatc
    74941 atttgtattc cagtaagcaa cgggccgatg atattgaggt ggtagtagct gcggaagccg
    75001 gtgaaaagga ttttgatcaa gaccaaccgc taaaagccgt gaatccccat ttggttgcca
    75061 aaggctatga gattgagaat cgtgggttta ctgattacgt gttgtatgtt gatgatttag
    75121 tgaaagcata ggaggaaaag aagatgagat taacagaagg tattgtcgta gattcaggtt
    75181 taacatttgg gaagttgcgt ttttccgcat tacgccgaga agtacggaag caaaatgagg
    75241 atggaacaat tggtaatgag gtaaaagaac gaacctataa tttaaaatcc tctgcacaag
    75301 ggcgaatgat tcaagttagt atcccagcta atgtaccctt acgcgagttt gcttatgatg
    75361 cagaggtaga gttggtcaat ccaatcgtgg ataccgttgc caattatgtt tttcgagaag
    75421 gaactaccgt taattggttc attaaggcgg atgatttagt cttgaagcgg cagccgaatc
    75481 aaggaaatcc tacgaaccaa aatgaaagaa agaaataggt gaagattatg gaaattaatg
    75541 ttgaacatat tttagattgt ctcgatcaat atggaaaagg cgagctgact gaggaacaac
    75601 tcgttaaagc attaacctct gatgaaaaaa tgtttctgat tatacatcaa ggtttacttg
    75661 gagaaaatag cgacagtgaa gaagactctg atgtactaga ctggttagga gaagaagagt
    75721 ctttctttat agttgtcgaa gtaaatgaaa acttatgtcg gcaggcagaa tccattttgg
    75781 aagaaattgg tgtggagatg ccggatgcta ttgaaggctt tttaaatcaa ttggttgaaa
    75841 cgaaacaact cccctcctcg tagcagtaaa tgactagcat aactttttga aattgcctaa
    75901 tgaaatccta ttgggctttt tcgattggag gtggaaacca tcaaaatgta caaaggacat
    75961 cgcatacgcg caggagatca gcacttggtt tatcactttg ttcttggctg gctactcgct
    76021 ctgttcatcg gttggatgag cgtcttttat tttcaagagt ttagacaatt tgatatttct
    76081 aagctatcac tttctactat tgaaatcgtt tggtctataa aagatttagt atgtctgctt
    76141 ggaagtctcg ctttttcagg agctatgata ttgctttata ttcacttttt tcttgatcat
    76201 tggcgaagct tgtggcatcg acaaaagcta gctcggatga ttctagagaa ccattggtat
    76261 gaagtgaaac aaactcaaag tgaaggcttt ttcaaagatt tgaatagtag ccgaaccaga
    76321 gagactatca gttacttccc caaaatctat tatcgaatga aagatggatt actgtctatt
    76381 cgcgttcaaa tttcacttgg gaaataccaa gatcagcttt taaaattgga aaagaagtta
    76441 gaaagcggac tgtattgtga gttagtagaa aaggaactca aagactccta tgtggagtat
    76501 actttactgt atgatatgat cgccaatcga attgggatag acgaagtagt ggcagaaaat
    76561 gggactctgc gattgatgaa aaatcaagta tgggcttatg attctttacc ccatatgtta
    76621 atcgctggtg gcacgggtgg agggaagacc tatttcctcc tcaccattat cgaagcacta
    76681 ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa atgcagattt agctgattta
    76741 ggtacggtga tgcctcatgt ttattcacaa aaggaagaaa tttctgcttg tgtggaagat
    76801 ttctatgaac gtatgatggc tcgtagcaag gcaatgaaag aaatgtctaa ctacaaaaca
    76861 ggagagaatt atgcctatct tggacttcca cccaactttt taatctttga tgaatacgtg
    76921 gcttatatgg agatgttaac gacgaaagaa agtgcggtga ttttgaataa gctcaaacaa
    76981 atcgtgatgt tgggtcgtca atctggtttc tttctgattt tggcttgcca aaggccggat
    77041 gcgaaatatt taggagacgg aatccgcgat ca
//
LOCUS       DACEJR010000006        74333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEJR010000006 DACEJR010000000
VERSION     DACEJR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.006"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     regulatory      208..305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            421..1002
                     /locus_tag="IW651_000412"
     CDS             421..1002
                     /locus_tag="IW651_000412"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0813171.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            1005..2312
                     /locus_tag="IW651_000413"
     CDS             1005..2312
                     /locus_tag="IW651_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0813172.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2728..3216
                     /gene="purE"
                     /locus_tag="IW651_000414"
     CDS             2728..3216
                     /gene="purE"
                     /locus_tag="IW651_000414"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0813173.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3200..4333
                     /gene="purK"
                     /locus_tag="IW651_000415"
     CDS             3200..4333
                     /gene="purK"
                     /locus_tag="IW651_000415"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0813174.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4346..5641
                     /gene="purB"
                     /locus_tag="IW651_000416"
     CDS             4346..5641
                     /gene="purB"
                     /locus_tag="IW651_000416"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0813175.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5821..6198)
                     /locus_tag="IW651_000417"
     CDS             complement(5821..6198)
                     /locus_tag="IW651_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813176.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6454..7182
                     /locus_tag="IW651_000418"
     CDS             6454..7182
                     /locus_tag="IW651_000418"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0813177.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            7182..7436
                     /gene="purS"
                     /locus_tag="IW651_000419"
     CDS             7182..7436
                     /gene="purS"
                     /locus_tag="IW651_000419"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0813178.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7438..8109
                     /gene="purQ"
                     /locus_tag="IW651_000420"
     CDS             7438..8109
                     /gene="purQ"
                     /locus_tag="IW651_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0813179.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            8110..10332
                     /gene="purL"
                     /locus_tag="IW651_000421"
     CDS             8110..10332
                     /gene="purL"
                     /locus_tag="IW651_000421"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0813180.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10317..11756
                     /locus_tag="IW651_000422"
     CDS             10317..11756
                     /locus_tag="IW651_000422"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0813181.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11788..12831
                     /locus_tag="IW651_000423"
     CDS             11788..12831
                     /locus_tag="IW651_000423"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0813182.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            12828..13406
                     /gene="purN"
                     /locus_tag="IW651_000424"
     CDS             12828..13406
                     /gene="purN"
                     /locus_tag="IW651_000424"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0813183.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13403..14938
                     /gene="purH"
                     /locus_tag="IW651_000425"
     CDS             13403..14938
                     /gene="purH"
                     /locus_tag="IW651_000425"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0813184.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14954..16228
                     /gene="purD"
                     /locus_tag="IW651_000426"
     CDS             14954..16228
                     /gene="purD"
                     /locus_tag="IW651_000426"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0813185.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16350..17264
                     /locus_tag="IW651_000427"
     CDS             16350..17264
                     /locus_tag="IW651_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0813186.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDGLERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17397..18962)
                     /locus_tag="IW651_000428"
     CDS             complement(17397..18962)
                     /locus_tag="IW651_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0813187.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18955..20315)
                     /locus_tag="IW651_000429"
                     /pseudo
     CDS             complement(18955..20315)
                     /locus_tag="IW651_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(20329..21345)
                     /locus_tag="IW651_000430"
     CDS             complement(20329..21345)
                     /locus_tag="IW651_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813188.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21565..22653
                     /locus_tag="IW651_000431"
     CDS             21565..22653
                     /locus_tag="IW651_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813189.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22707..24233
                     /locus_tag="IW651_000432"
     CDS             22707..24233
                     /locus_tag="IW651_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813190.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24235..25440
                     /locus_tag="IW651_000433"
     CDS             24235..25440
                     /locus_tag="IW651_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813191.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25697..26674
                     /gene="guaC"
                     /locus_tag="IW651_000434"
     CDS             25697..26674
                     /gene="guaC"
                     /locus_tag="IW651_000434"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0813192.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26938..28362
                     /locus_tag="IW651_000435"
     CDS             26938..28362
                     /locus_tag="IW651_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0813193.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28823..29932
                     /gene="pdhA"
                     /locus_tag="IW651_000436"
     CDS             28823..29932
                     /gene="pdhA"
                     /locus_tag="IW651_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0813194.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29935..30912
                     /locus_tag="IW651_000437"
     CDS             29935..30912
                     /locus_tag="IW651_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0813195.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            30937..32580
                     /locus_tag="IW651_000438"
     CDS             30937..32580
                     /locus_tag="IW651_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ0813196.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            32588..33994
                     /gene="lpdA"
                     /locus_tag="IW651_000439"
     CDS             32588..33994
                     /gene="lpdA"
                     /locus_tag="IW651_000439"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0813197.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            34156..34431
                     /locus_tag="IW651_000440"
     CDS             34156..34431
                     /locus_tag="IW651_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0813198.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            34437..35204
                     /locus_tag="IW651_000441"
     CDS             34437..35204
                     /locus_tag="IW651_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0813199.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            35372..37201
                     /gene="typA"
                     /locus_tag="IW651_000442"
     CDS             35372..37201
                     /gene="typA"
                     /locus_tag="IW651_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0813200.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            37462..39234
                     /gene="recQ"
                     /locus_tag="IW651_000443"
     CDS             37462..39234
                     /gene="recQ"
                     /locus_tag="IW651_000443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0813201.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            39348..39650
                     /locus_tag="IW651_000444"
     CDS             39348..39650
                     /locus_tag="IW651_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0813202.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            39653..40816
                     /locus_tag="IW651_000445"
     CDS             39653..40816
                     /locus_tag="IW651_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0813203.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            40842..44270
                     /locus_tag="IW651_000446"
     CDS             40842..44270
                     /locus_tag="IW651_000446"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0813204.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            44291..45415
                     /locus_tag="IW651_000447"
     CDS             44291..45415
                     /locus_tag="IW651_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813205.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            45405..45842
                     /locus_tag="IW651_000448"
     CDS             45405..45842
                     /locus_tag="IW651_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0813206.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            45826..46164
                     /locus_tag="IW651_000449"
     CDS             45826..46164
                     /locus_tag="IW651_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0813207.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            46283..46843
                     /gene="rsmD"
                     /locus_tag="IW651_000450"
     CDS             46283..46843
                     /gene="rsmD"
                     /locus_tag="IW651_000450"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0813208.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            46840..47331
                     /gene="coaD"
                     /locus_tag="IW651_000451"
     CDS             46840..47331
                     /gene="coaD"
                     /locus_tag="IW651_000451"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0813209.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            47321..48364
                     /locus_tag="IW651_000452"
     CDS             47321..48364
                     /locus_tag="IW651_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0813210.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            48523..49251
                     /locus_tag="IW651_000453"
     CDS             48523..49251
                     /locus_tag="IW651_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0813211.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            49367..49612
                     /locus_tag="IW651_000454"
     CDS             49367..49612
                     /locus_tag="IW651_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0813212.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            49609..49827
                     /locus_tag="IW651_000455"
     CDS             49609..49827
                     /locus_tag="IW651_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0813213.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            49963..50502
                     /locus_tag="IW651_000456"
     CDS             49963..50502
                     /locus_tag="IW651_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0813214.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            50495..52768
                     /locus_tag="IW651_000457"
     CDS             50495..52768
                     /locus_tag="IW651_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0813215.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            52806..53852
                     /gene="holA"
                     /locus_tag="IW651_000458"
     CDS             52806..53852
                     /gene="holA"
                     /locus_tag="IW651_000458"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0813216.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(53941..54195)
                     /locus_tag="IW651_000459"
     CDS             complement(53941..54195)
                     /locus_tag="IW651_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0813217.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(54595..54863)
                     /locus_tag="IW651_000460"
                     /pseudo
     CDS             complement(54595..54863)
                     /locus_tag="IW651_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(55333..55788)
                     /locus_tag="IW651_000461"
     CDS             complement(55333..55788)
                     /locus_tag="IW651_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0813218.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(55926..57854)
                     /locus_tag="IW651_000462"
     CDS             complement(55926..57854)
                     /locus_tag="IW651_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0813219.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(58051..58884)
                     /locus_tag="IW651_000463"
     CDS             complement(58051..58884)
                     /locus_tag="IW651_000463"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0813220.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(59076..59780)
                     /locus_tag="IW651_000464"
     CDS             complement(59076..59780)
                     /locus_tag="IW651_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0813221.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(59803..60429)
                     /locus_tag="IW651_000465"
     CDS             complement(59803..60429)
                     /locus_tag="IW651_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0813222.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            60755..63130
                     /locus_tag="IW651_000466"
     CDS             60755..63130
                     /locus_tag="IW651_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0813223.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(63154..63645)
                     /locus_tag="IW651_000467"
     CDS             complement(63154..63645)
                     /locus_tag="IW651_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0813224.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            63832..64752
                     /locus_tag="IW651_000468"
     CDS             63832..64752
                     /locus_tag="IW651_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0813225.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            64906..65250
                     /locus_tag="IW651_000469"
     CDS             64906..65250
                     /locus_tag="IW651_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0813226.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(65440..65940)
                     /locus_tag="IW651_000470"
     CDS             complement(65440..65940)
                     /locus_tag="IW651_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813227.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            66468..66551
                     /locus_tag="IW651_000471"
     tRNA            66468..66551
                     /locus_tag="IW651_000471"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66502..66504,aa:Leu,seq:cag)
     gene            complement(66875..67513)
                     /locus_tag="IW651_000472"
     CDS             complement(66875..67513)
                     /locus_tag="IW651_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0813228.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            67848..68282
                     /locus_tag="IW651_000473"
     CDS             67848..68282
                     /locus_tag="IW651_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0813229.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            68305..68625
                     /locus_tag="IW651_000474"
     CDS             68305..68625
                     /locus_tag="IW651_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813230.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            68766..68957
                     /locus_tag="IW651_000475"
     CDS             68766..68957
                     /locus_tag="IW651_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813231.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(69028..69285)
                     /locus_tag="IW651_000476"
     CDS             complement(69028..69285)
                     /locus_tag="IW651_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0813232.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            69443..69706
                     /locus_tag="IW651_000477"
     CDS             69443..69706
                     /locus_tag="IW651_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813233.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            69730..69918
                     /locus_tag="IW651_000478"
     CDS             69730..69918
                     /locus_tag="IW651_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813234.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(70001..70186)
                     /locus_tag="IW651_000479"
     CDS             complement(70001..70186)
                     /locus_tag="IW651_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813235.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            70401..70772
                     /locus_tag="IW651_000480"
     CDS             70401..70772
                     /locus_tag="IW651_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0813236.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(70922..70994)
                     /locus_tag="IW651_000481"
     tRNA            complement(70922..70994)
                     /locus_tag="IW651_000481"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70959..70961),aa:Gln,seq:ctg)
     gene            complement(71040..71468)
                     /locus_tag="IW651_000482"
     CDS             complement(71040..71468)
                     /locus_tag="IW651_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813237.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(71528..72286)
                     /locus_tag="IW651_000483"
     CDS             complement(71528..72286)
                     /locus_tag="IW651_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0813238.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            72581..72778
                     /locus_tag="IW651_000484"
     CDS             72581..72778
                     /locus_tag="IW651_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813239.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            72884..73366
                     /locus_tag="IW651_000485"
     CDS             72884..73366
                     /locus_tag="IW651_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813240.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(73424..74020)
                     /locus_tag="IW651_000486"
     CDS             complement(73424..74020)
                     /locus_tag="IW651_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0813241.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT        24397 a        12678 c        15876 g        21382 t
ORIGIN      
        1 agaaaataaa aataactatt taaacccgaa caaaaaggaa taatgcatta aaaatcatta
       61 gattttacaa aaaaataagt aaaccacgtt gtcagtttca gaaagtagtg ttagactgtt
      121 ttcaatattt gaaacaacat tttttattca aatatggcaa actgccttca cgtgcaaaac
      181 agttgcaaga aaatgaattt acactattat tagttttaca tacctactta tatatcgtca
      241 taatatggat gacagtttct agccagtacc gtaaatgctg gactataagt aaaagattgg
      301 ctatttcacc cttcttttct tatgataaag tggagggtgt ttttctttaa cagaaacata
      361 gtaggttttt gcacaaaagc ggttatttcg gtattaataa atgataaagg agcaaataaa
      421 gtgaaagaat tagttgaacg tattcaaaaa gacggacgta tattaggcga gggcgtatta
      481 aaagtcgaca gcttcgtcac tcaccaagta gaccctgtat tgatggaaca aatgggaaaa
      541 cggtttgcag aagtatttaa agaacaagga atcacgaaag ttgtgaccat tgaagcttct
      601 gggatcgcac cagctttatt tgctgcaaga gaattagatg ttccaatgat cttcgctaga
      661 aaagcaaaaa gtttgacgat ggatgaagaa ttattgacag cttctgttta ttcttttacg
      721 aaacaagtga caagtacgat ttctatttct cgtaaatttt tatcttcaga agacaaagta
      781 ttgattatcg acgatttctt ggcaaacgga caagcggcaa aaggattgat cgaattatgc
      841 cagcaagctg gagcgcaagt agaaggaatc gggatcgtca tcgaaaagtc tttccaagat
      901 ggacgtcagt tacttgaaga aatgggattg agagtcgttt ctcttgcgcg tattgcttcc
      961 ttgacgaatg gacaagttga gtttcttgag gaggacgcat aatcgtggaa aaagaaacac
     1021 aaaatggaaa agctgcggtt ttagggctgc agcatctgct agcgatgtat gcaggagcag
     1081 tagcggtgcc tcttttgatc ggtacaggac taggctttaa tcaagaacag atgacttatt
     1141 tgatttcaat tgatatcttt atgtgtggat tagcgacatt attgcaattg acagttaacc
     1201 gatttttcgg aattggcttg cctgtcgttt taggatgtgc gatccaagca gtagctccac
     1261 tgatcttgat cggaaccaat gaaggagtag gagctatcta cggatctatt atcgcttctg
     1321 ggatctttgt tgtgttagtt tctggtgtct tttctaaaat taaaaaacta ttccctccta
     1381 tagtaacagg aacggttatc accgttattg gtttgacttt gattcctgta gcaatcgaga
     1441 aaatgggtgg aggtaatgca acagcagctg attttggtga caaaacaaat ctgctacttg
     1501 catttgtgac gattctccta attgtaggcg tgcaaatgct ggcaaaaggg ttcattcgtt
     1561 caatcgcggt attgattggt ttagtcggag gaagcatctt agctgctttc atgggaatga
     1621 ttgatctgag cgcgattgga gatgcgccgt tgttccatgt tccacaaccc ttttattttg
     1681 gtaaaccaac tttcgacgta tggtcgattt tactaatgat catcatttcc atggtcagca
     1741 tggtagaatc aacaggtgta tattttgcac ttggtgacat tactgggaaa aatgttggag
     1801 aagaagaatt gaaaaaggga tatcgtgcag aaggacttgc cgttatctta ggcggtattt
     1861 tcaatacatt cccttatact ggcttttctc aaaatgtcgg attggttcaa ctttcgggaa
     1921 tcaaaacaag aaaacccatc tatttctctg cattcttctt gattgtttta ggattattcc
     1981 ctaaaatcgg tgcgttagca caaatcatcc ctgagccggt acttggtgga ggaatgttag
     2041 tcatgttcgg gatggtggca gtccaaggaa tccgtatgct gttggaagtt gatttcagca
     2101 atgataaaaa tctattgatt gttgctgttt ctgttggatt tgggctgggt ttcaatgtta
     2161 cgccgacatt attcagtcaa atgccgcaaa cattgcaaat gttcactgga aatggcatcg
     2221 taatgagtag cattacggct atcatattga atttgatctt taatggattg aaaaaagatg
     2281 aagaaatatt ggaagaagcc ttcaagaagt aatagagcaa aagaaaggtt gtgaaatcag
     2341 cgttttgacc tgattgttag agaaacaaac ggaaaaatag ttcctcgtat tttgccgttt
     2401 gtttgagcta ataccgaggg tcagctgatt gaacaccgtt gaaggaagag gtcgtgagcc
     2461 tgccgttcat ctccgagtca caaggaacaa tttcaaaaaa aagcttctca tgtttgttga
     2521 atattgttcc ttgtgaccga ggagttggca ggagaacacc gtttatccat aaaaaggaat
     2581 gtgacctaac ttttgtcaca ttcctttttt ttatctttaa taaacacgaa caataaaatg
     2641 tttttctttg ttaacattcg aaatttcttt gacatcaaaa aaagtgattg ttaaaatatg
     2701 aatgtagaaa aactgggagg aaaaacaatg acagcaaaaa tctctgtgat tatggggagt
     2761 acttcagatt gggaaacaat gaaagaagca tgtcaaatat tggatgaatt cggtgtgtct
     2821 tatgaaaaaa aggtcgtttc tgctcatcga accccagatt tgatgtttga atatgcacaa
     2881 aacgctcgaa aaaaaggaat caaaatcatt attgcaggag cagggggagc cgcgcatctg
     2941 ccgggtatgg tcgcatcaaa aacaactttg ccggttatcg gtgtaccggt ccaatcacga
     3001 acattaaatg gtttggattc gcttctttcg attgtccaaa tgcctggagg agtacctgta
     3061 gctactacag caatcggaaa agcgggagca attaatgctg gactacttgc gatacagatg
     3121 ctttccatgt atgatttaga attggaggct aaattggctg aaagacgttc aatgttggct
     3181 aaaactgtta tagaaagtag tgatcaactt ggttaaacct ttgttgcctg gttcaacgat
     3241 cgggatcgtc ggtggcggtc aattaggtcg tatgatggcg attagtgcaa aagaaatggg
     3301 ctttaaagta ggtgtgctgg atcctgtgag cgattgtcca gccgcacaag ttgcagattg
     3361 gcacattgaa agtgtctatg atgacacctt tgcgctgaaa gagcttgcca gaagaacaga
     3421 tgtcattacc tatgaattcg aaaacgtcag tatagaggcg ttaaatgcga ttttacccat
     3481 gtcatttatt cctcaaggga cagacttgct tgcgattacg caagaccgcc tgttagaaaa
     3541 atcttttttg gaaacgaata acatcgtgat tgctccttat gcaacgatcg tcagtccgac
     3601 ggatatccaa gatgcgattg acgggatcgg ctatccttgt gttttgaaaa ccactcgagg
     3661 cggctatgat ggcaaggggc agtatgtttt gaaaagtcgg gcggatctag ctcctgccat
     3721 ggacctttta agagaaggaa cctgtgtttt agaagcatgg atcccgtttg aaaaagagat
     3781 ttcgatcatg gtggcaggaa atggtcaagg agatttcacg acttttccgg tcgtggaaaa
     3841 catccaccac aacaatattc ttcatgaaac gattgctcca gccgcgattg atcaggatgt
     3901 gatcgaggag gctgagcgta ttgcccgtgt gattgcagaa gcagtgagcc tttcaggtgt
     3961 tttaggggta gaaatgtttt taacaaaaac aggcgggcta tatgtgaatg aattagctcc
     4021 tcgtcctcac aattctggac attattctat tgaagcttgt tctatgagtc agttcgatgc
     4081 ccatattcga ggtatttgtg gttggccatt aggagaaaaa gtacgtttga taagcaatgc
     4141 agtaatggtc aatgttttgg gagaacaact gctggaaagc tatcgcttga ttgagaataa
     4201 gccagactgg caattccatt tctatggaaa aaaacaagca aaaaaaggtc gcaagatggg
     4261 acatatcact atcttgacag aagacatcca gcaaacgctg gaagaaatca aacaaacgaa
     4321 tatttgggat tagaggagaa aaaacatgtt agaacgttat acacgccctg aaatggggaa
     4381 tatctggacg gaagagaaca aataccgtgc gtggctcgaa gtcgagattt tagcagatga
     4441 agcttgggca gaacttggtg aaatcccgca agaagatgtg aaaaaaattc gtgagcatgc
     4501 atcatttgat atcgatcgga tcttggaaat cgaaaaatta acacgccatg atgtcgttgc
     4561 gtttacacga gcggtttctg aaacattggg agaagaaaga aaatgggtcc attatggtct
     4621 aacaagtacg gatgtcgtag atactgctta tggctatctc atcaagcaag caaatgatat
     4681 tttacgggaa gacttgaaac gttttacgga aatcattggc gaaaaagcaa aagaacacaa
     4741 atatacagtg atgatgggtc ggacacacgg tgtccatgct gaaccaacta cttttggatt
     4801 gaaacttgcc acatggtatt cagaaatgaa acggaacatc gaacgatttg aacatgcggc
     4861 aaaaggagta gaagcaggaa aaatctcagg agcagtggga acgttcgcaa atatcccgcc
     4921 atttattgaa gagtatgtat gtaaacaatt ggggatccgt ccacaagaaa tttctactca
     4981 agttctccct cgggatctac atgctgaata tttctcagcg atggcgctta ttgctacaag
     5041 tattgaacgt tttgcaacag agatacgcgg attgcaaaaa tccgaaacac gtgaagtaga
     5101 agaatttttt gcgaaaggtc aaaaaggttc ttctgctatg ccgcacaaaa ggaatccgat
     5161 cggttcagaa aatatggctg gtctagccag agtggtgcgc ggacacatgg tcactgccta
     5221 tgaaaatgtc acattatggc atgaaagaga tatttctcat tcttcagcag aacgaatcat
     5281 cttgcctgat accacgattt tattgaatta catgttaaat cgttttggca atatcgttaa
     5341 aaatctaacg gtctttcctg aaaacatgaa gcggaatatg tctgcaacat ttggtttgat
     5401 ttatagccag cgcgttatgc tgaaattgat cgataaggga atgacaagag aagaagctta
     5461 tgatcttgtt cagccaaaaa ctgcgtacgc ttgggacaac caagtgcagt tccgcccgct
     5521 gttagaagaa gatccaacca tcacatcggt attatctaaa gaagaaatag acgatgcctt
     5581 tgattatcat catcatttga aaaacgttga cgtgatattc aaacgagtag gtttggaata
     5641 aagaagagtg agttgcaaaa ttcaattaaa atagaatagc aaaaataacc aaaaaagaag
     5701 cagatccgtt agcggttctg cttcttttag tttattatat tttcttatca ataataggaa
     5761 cctgatttta aaagcggcta cctctttacc gtgcctcttt acatacaaaa aatagaggaa
     5821 ttaagcgtaa atggcttggc tattataact aaaatgctgg ttatggagat gttgcagcaa
     5881 gttttcgaga aaagcgtatt ccatctcttg tccattgaat aagatctgga tacgataatg
     5941 atttttgaaa cgcttctgtt catgaatctg actgattgaa agttcgcctt tgtgttctgt
     6001 ctgataaaga aaagtccttt tacggcattc agcacagcgc ttggttgctc ggcggatttt
     6061 tcggaatgta taaaaactag tatctttatt gcataaataa acgtctttga tcgacagcaa
     6121 atcattatat agacaggaac gttcttcagc tgttaattct gataaaaaaa gctgaccttg
     6181 ttttattgca tcttccatcg gcaagtgcct cctcgaaccc attatccaaa tttttagtta
     6241 attctaatat accatcatta agtataaatg taaacgcttg ttgatggtat aagattttcg
     6301 tttctgataa aacacgaaca ttaaaagaat ctaatgcctt aaatgtacat atttttgttg
     6361 atttataaaa agatgattgc taaaatttaa agtgaaaaaa cgaacaaaaa acgatgtatt
     6421 tgcttttaat gtaaataaaa aggagagacg tacatggaga agaaagaact tttgtatgaa
     6481 ggaaaagcca aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta
     6541 gatcaagcaa ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg
     6601 aataatcaaa tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat
     6661 tttattgaac aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg
     6721 gaagtggtta tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa
     6781 ggtaaagaat tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat
     6841 ccatttatca atgaagagca tatctatttc ttacaacttg caaagccgga agaaatcagt
     6901 gagctgaaag cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc
     6961 ggtttggttt tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt
     7021 gcggatgaag taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg
     7081 gacaaagacg tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat
     7141 aaccgtctat cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat
     7201 atgtaaccta taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc
     7261 atcgtatggg atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac
     7321 ttgctgatgc agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg
     7381 caaatgtaaa tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg
     7441 aaatttgcgg tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt
     7501 cgcgagatca tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga
     7561 tttgatgggg ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg
     7621 attgctcggt tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca
     7681 gtattcggta catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta
     7741 ctgagacgaa atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc
     7801 aagacggcct ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat
     7861 ggggaaggaa attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag
     7921 attgccttta cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg
     7981 aacaaagcag ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg
     8041 ctaggatcag aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg
     8101 accgtataga tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc
     8161 tatgccgaat ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga
     8221 agaatgccta attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc
     8281 tataaaaatt ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa
     8341 ggccctggag aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa
     8401 gcagaaagtc acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt
     8461 ggcggaatca tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca
     8521 ttgaggttcg gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca
     8581 ggtatcggcg gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt
     8641 gatccttgtt atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac
     8701 aaacatatcc agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca
     8761 aaaacaggtc gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa
     8821 gaagaacagc aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt
     8881 gaagcgtgtt tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg
     8941 ggagcagcag gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt
     9001 atattgacat tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg
     9061 ttatcggaat cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc
     9121 caagaactat ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac
     9181 gggcaatatc ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta
     9241 gctgaagatg caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt
     9301 cagcagttag ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg
     9361 ctgcagcaac cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta
     9421 caaaccaata cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg
     9481 aataaagcgt tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa
     9541 atcggaggac aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag
     9601 cccttggcga tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg
     9661 gaattatcga cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt
     9721 atttcaggaa atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca
     9781 atgattggta tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa
     9841 aaatcagggg atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa
     9901 ttacaaaaga tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt
     9961 gaaaagagaa accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct
    10021 catgactgtt cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag
    10081 ctagggatat ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca
    10141 agatttgtgt tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat
    10201 gctgctgttt tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac
    10261 ggtcagatca aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt
    10321 cttatgaagt gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag
    10381 acgccgctag tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg
    10441 caggtatcgt gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt
    10501 ctgaggtatt caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac
    10561 atgtacgtta tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca
    10621 aatttcatga tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc
    10681 ttagaaaaga gttagaggaa aatggggcga tcttccattc taactccgat acggagatat
    10741 tgatgcattt gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt
    10801 tgaatgaagt caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg
    10861 ctttggaccc aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg
    10921 tgattgcctc ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc
    10981 agcctggtga gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg
    11041 acacgcagct ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca
    11101 tcgcagggat caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat
    11161 cgccagtaaa agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg
    11221 gttatgcgga agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg
    11281 cccgaacatt tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat
    11341 ctgctgtacg aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc
    11401 ggggtacgac aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc
    11461 atgtgaggat tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa
    11521 cgcgtaaaga actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg
    11581 cagattcttt aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag
    11641 atgctccgta ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat
    11701 atgactatga agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc
    11761 agaccaagaa gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat
    11821 gtcgaagctg gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt
    11881 ttaggtatga tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa
    11941 ttaaaagagc cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca
    12001 atcgaagcag acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat
    12061 atcttagcac aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat
    12121 gatccgttac gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat
    12181 atggcgctga ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat
    12241 gatctagccg gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg
    12301 atcaacccag gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac
    12361 tctcttgttc gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca
    12421 gaattgaaaa aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc
    12481 ttgcttccgc tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg
    12541 tttgtagaaa atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc
    12601 tcatggccga ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg
    12661 gagatgtatg aaatcttcaa tatgggcatc ggtatggtcc tggcagtcga agaatcgaaa
    12721 gtaaaagaaa tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa
    12781 gtgatcgcca aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg
    12841 tttttgcttc tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag
    12901 gattggaatc ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac
    12961 gtgcaactgc gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa
    13021 aagagtatga agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat
    13081 tagctggata tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca
    13141 tcaatatcca tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct
    13201 ttgaagctgg agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata
    13261 ctggaccaat catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt
    13321 tagaagaaaa aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta
    13381 ttgaaaatgg aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg
    13441 gattgtagca ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg
    13501 aacaaagcat gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg
    13561 gtttcctgaa atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct
    13621 tttaggaaga cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc
    13681 aatcgacttt gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt
    13741 cgaaatagca gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc
    13801 agcaaaaaac catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact
    13861 ttcagaatta cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa
    13921 agtctttcgt catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt
    13981 ggaagaaaaa gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta
    14041 cggtgaaaat agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc
    14101 gatcgcagca gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc
    14161 agatgctgcg ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca
    14221 catgaatcct tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta
    14281 tgaagcagat cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct
    14341 gccaacagca gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac
    14401 acaagaagct ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt
    14461 tgcgcaggaa gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt
    14521 acaagatcaa gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa
    14581 gccgactcca gcagaagcaa aagcattagt gtttgcttgg agagctgtca aacatgtgaa
    14641 atctaatgcg atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat
    14701 gaatcgagtc ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc
    14761 agttttagct agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca
    14821 tggtataaaa gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat
    14881 ggcgaataaa tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag
    14941 aggagtcgga taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag
    15001 taaaaaactt ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg
    15061 gatgacaaaa gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat
    15121 ccattttgtt aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt
    15181 aaatgggatc gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc
    15241 agcagctctg atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat
    15301 cccgacagct aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga
    15361 aaatggtgcg ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt
    15421 ggctaccaca gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt
    15481 tggggatagt agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct
    15541 tttggcgttt gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg
    15601 ggcatttgat aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc
    15661 gcaaatttca aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt
    15721 gggaatgaaa gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac
    15781 gaaggaagga ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt
    15841 cgttctttct cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg
    15901 tacagtagag ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc
    15961 cggttacccc gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct
    16021 ttctgtttac tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg
    16081 tctttattta gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac
    16141 cgcgttaatg gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc
    16201 tgccaatagc gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca
    16261 aacataatcg gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt
    16321 gttgtgtata taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc
    16381 agaaagcagc tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa
    16441 aagtgattga gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg
    16501 gaaacggtga atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag
    16561 aagatctttt tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta
    16621 aaaaacatat cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg
    16681 atgctttact tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt
    16741 ttgatggatt ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa
    16801 tgcaaatcga attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat
    16861 ttggcttgaa acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag
    16921 caagtatcga tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc
    16981 aagcatggtc accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat
    17041 tccctgaatt aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg
    17101 gcttggcttc tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga
    17161 tgaacgtagg gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag
    17221 attggtacga attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag
    17281 gtcaaaaggg ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga
    17341 tattctgtct ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag
    17401 ttatttttgt ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt
    17461 ttcttaaata aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct
    17521 gaaatacgat agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc
    17581 agataatcga tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga
    17641 cttaatgcaa cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct
    17701 ttgataaaat ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag
    17761 ggcagagcag ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg
    17821 gcgctgattg ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc
    17881 ttttctttca ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt
    17941 gttcgagttg gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt
    18001 ttcgataatg aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt
    18061 ttcgcttctt gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt
    18121 tcaagtatca tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca
    18181 ggaccaatgg cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt
    18241 tgggctacct gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat
    18301 ttgatgaatc gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac
    18361 ttcaaataat cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct
    18421 aataatacgg tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat
    18481 gtttcactga tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat
    18541 tcgaaaacaa gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact
    18601 gctttttcta ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct
    18661 gcctgtacct ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg
    18721 agacgagtcc aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa
    18781 ggcatttgga tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc
    18841 atctcaccat ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt
    18901 atttgttccc gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac
    18961 atgtttgttt cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc
    19021 ttctcggtat ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag
    19081 acgcttgttt acgttgtaaa gtccgtaact ttgaattgga tcatctcatc aattttgcat
    19141 gtggtaatac gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt
    19201 tcaatcaatg gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata
    19261 tcgatcttat agtcaagcat atggccgtat cttattttta agatgactag ataatcattg
    19321 atatggctaa tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga
    19381 aaaaagccgg aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc
    19441 aaagaaataa tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat
    19501 acacgaactt cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg
    19561 tttagatctt ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc
    19621 ggcagggtta aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc
    19681 tgattgatcg gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa
    19741 aaaatggtac taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt
    19801 gtcgtatttt ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct
    19861 gacagcagtt gagtgacaga atctacctga ttgatgatct cttcaatggt atccataaaa
    19921 tcttttgcca agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta
    19981 cgtagcgata catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct
    20041 cgaatatcat tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact
    20101 tggccagaaa tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt
    20161 gcatctgctt tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat
    20221 aacagtatgc tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg
    20281 gcaaaaatct gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct
    20341 ggttgggatg ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt
    20401 aggtaatgtt tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata
    20461 ccagccgaca tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc
    20521 gatcatatct tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga
    20581 tgtttctttt atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac
    20641 atcgatttcg gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat
    20701 ccgaataaaa tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt
    20761 tccatctttt tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag
    20821 cttggtcggt gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt
    20881 tgaatttgtg aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact
    20941 ggggccaatg tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag
    21001 tatgacaggg atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg
    21061 caaaggagta aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat
    21121 ttgccgttcc tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag
    21181 cgcagtgata attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga
    21241 aaaaccaacg actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg
    21301 gtaagcgatt acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat
    21361 ctggatttgt tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa
    21421 tcgaaaaaaa tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt
    21481 taaagtaaat cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt
    21541 atcaattgaa atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc
    21601 gctattttga ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat
    21661 agtacggggt atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat
    21721 gggaacaaca tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga
    21781 gaagataaag tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat
    21841 acacttgtga ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa
    21901 gaagcagata tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat
    21961 tattacgcga cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag
    22021 cacttaggat tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct
    22081 gacgacaaca acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg
    22141 gacaacggtc agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt
    22201 cgctgggatg gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact
    22261 gaccaaacat tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg
    22321 tcattaaaag gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa
    22381 gatgcgacag ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt
    22441 aaagacacac gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga
    22501 gaagaggttc cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg
    22561 tatttagcaa atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc
    22621 gattattata aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga
    22681 tctaagtcag agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca
    22741 ccaaaaaatt ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg
    22801 aaatccatgc attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga
    22861 gcggcttgta tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc
    22921 aatttaaaga tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag
    22981 cactaagtcc ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa
    23041 gaggcgtgat tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg
    23101 gtttgtctat cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag
    23161 ttgaaatagc taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg
    23221 ctgcattgaa tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc
    23281 aaggtgtaac ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc
    23341 gaatcaccat tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg
    23401 aagagcatat tattcgcgga atggtgggca gagatttagt tgatcgttat cctgaaagaa
    23461 aaccggatat tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg
    23521 atcacgagcg aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag
    23581 gaatagctgg actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt
    23641 cttatggaag caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga
    23701 ccgttccttc agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag
    23761 gactgaatct gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa
    23821 gtcaaaaagg agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa
    23881 agatgcgtat aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc
    23941 aacaaaaagt cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg
    24001 agccaacccg tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa
    24061 tggcagcaaa tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga
    24121 tgtgtgatcg aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag
    24181 atgcagatca ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa
    24241 caggcaaaaa atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct
    24301 gatctttttg gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa
    24361 ttgatgacgg atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat
    24421 agccatatat tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat
    24481 ttgtcggtag ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat
    24541 cgccaaatga acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc
    24601 gcatggcaag gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca
    24661 ggattgctga tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct
    24721 tttccagctg ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc
    24781 agccttcact tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt
    24841 caatggcgaa caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct
    24901 ttcttggtaa aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc
    24961 tcttatcaag ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg
    25021 tttttgacaa ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa
    25081 gcggctgaac tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg
    25141 ggagtcatgg cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct
    25201 caagcaggta catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg
    25261 acttccggcg gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta
    25321 ttgaacaatg gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga
    25381 ttgatcttgc ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag
    25441 gagaacgccg gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc
    25501 gctaaaagcg aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta
    25561 ccattcgtat aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat
    25621 agggcgctaa aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca
    25681 tcaggaggat aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa
    25741 atgtatcgta aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt
    25801 caaaatgcca gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt
    25861 cttagcggaa aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc
    25921 gtttatcaaa aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa
    25981 agaggaatat tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac
    26041 tatcgacatc gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa
    26101 atatttgcct gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga
    26161 attggaaaat gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat
    26221 tacaaaaatc aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg
    26281 tgcgaaagca gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat
    26341 tgcaaaatct gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca
    26401 cgaggaatct cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg
    26461 ttctgcttca gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat
    26521 ccaacacaaa ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc
    26581 tatttcttat gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt
    26641 gaaaaattcg atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg
    26701 tgaaaaagtt gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg
    26761 ttttgtttgg ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata
    26821 agggactgtg ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt
    26881 tttctcgtaa attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg
    26941 tgggaaaaag tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg
    27001 agagaaatcg aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta
    27061 gggcaacttt ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag
    27121 cttggggtac taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg
    27181 aaattcgctg ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg
    27241 gactggtatg agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat
    27301 ttatacgcta gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct
    27361 cttgaagaaa tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt
    27421 aacaaaatca aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt
    27481 cttgtgactg gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct
    27541 ggagagggga tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct
    27601 tcaggaacgc gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg
    27661 aaagaatcga tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac
    27721 cctacaggaa aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag
    27781 tatcggcttc caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa
    27841 gaagtccttc ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct
    27901 agggttttag gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg
    27961 caactagctc aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca
    28021 gtggctaatg tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg
    28081 aaagaaaaaa gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat
    28141 tcaattttag gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa
    28201 ctagcagaaa aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa
    28261 gatataaatg gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt
    28321 tattcaaaga tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga
    28381 ttaaagaata taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga
    28441 atacgatata aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa
    28501 taaaataaat gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg
    28561 ttgtaagttt gattagaaaa tagaaaaaat gcagatgaaa tataaaacgc tttccttatt
    28621 gtgattttat tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa
    28681 aatttgtgct tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt
    28741 taaatagtga tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag
    28801 atgagagagg aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact
    28861 gctagatgaa gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt
    28921 agttaacaaa gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca
    28981 tatggtatgg tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct
    29041 aggctttttt gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt
    29101 tgataaagaa gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg
    29161 tttaccttta tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc
    29221 ggaagaattg aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc
    29281 cggtgttgca ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg
    29341 tgatggcggt tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca
    29401 tgcaaatgcc gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa
    29461 acaaactgca gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca
    29521 agtcgatgga atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc
    29581 gatcagtgga aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac
    29641 actatctggt gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa
    29701 aaaagatcca ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga
    29761 aaaagaagaa caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc
    29821 cgacaaagta ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc
    29881 acaatcaatc aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca
    29941 caaaaaacaa tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag
    30001 aatgtcgttg tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa
    30061 ggattgcagg aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga
    30121 atcgcaggat tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa
    30181 ttctttggtt ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga
    30241 tatcgaatga gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt
    30301 gttcatacac cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt
    30361 attcgagtag tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt
    30421 agaagtaacg atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa
    30481 gaagtgccgg atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga
    30541 acagatgtat ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac
    30601 aatcttgcaa aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta
    30661 gatgtagaaa cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa
    30721 gcacaacgac aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc
    30781 ctttcattag aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga
    30841 caagcagaaa atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc
    30901 gcggaatttt aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaattgccg
    30961 gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat
    31021 acgatcaatg aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatc
    31081 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
    31141 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
    31201 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
    31261 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
    31321 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
    31381 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
    31441 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
    31501 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
    31561 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
    31621 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
    31681 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
    31741 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
    31801 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
    31861 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
    31921 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
    31981 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
    32041 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
    32101 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
    32161 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
    32221 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
    32281 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
    32341 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
    32401 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
    32461 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
    32521 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
    32581 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
    32641 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
    32701 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
    32761 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
    32821 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
    32881 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
    32941 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
    33001 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
    33061 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
    33121 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
    33181 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
    33241 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
    33301 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
    33361 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
    33421 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
    33481 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
    33541 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
    33601 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
    33661 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
    33721 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
    33781 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
    33841 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
    33901 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
    33961 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
    34021 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
    34081 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
    34141 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
    34201 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
    34261 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
    34321 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
    34381 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
    34441 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
    34501 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
    34561 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
    34621 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
    34681 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
    34741 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
    34801 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
    34861 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
    34921 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
    34981 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
    35041 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
    35101 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
    35161 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
    35221 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
    35281 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
    35341 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
    35401 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
    35461 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
    35521 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
    35581 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
    35641 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
    35701 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
    35761 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
    35821 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
    35881 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
    35941 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
    36001 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
    36061 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
    36121 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
    36181 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
    36241 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
    36301 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
    36361 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
    36421 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
    36481 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
    36541 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
    36601 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
    36661 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
    36721 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
    36781 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
    36841 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
    36901 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
    36961 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
    37021 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
    37081 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
    37141 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
    37201 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
    37261 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
    37321 gccgaaaggc gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
    37381 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
    37441 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
    37501 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
    37561 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
    37621 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
    37681 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
    37741 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
    37801 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
    37861 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
    37921 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
    37981 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
    38041 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
    38101 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
    38161 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
    38221 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
    38281 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
    38341 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
    38401 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
    38461 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
    38521 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
    38581 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
    38641 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
    38701 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
    38761 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
    38821 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
    38881 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
    38941 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
    39001 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
    39061 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
    39121 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
    39181 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
    39241 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
    39301 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
    39361 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
    39421 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
    39481 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
    39541 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
    39601 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
    39661 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
    39721 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
    39781 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
    39841 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
    39901 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
    39961 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
    40021 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
    40081 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
    40141 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
    40201 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
    40261 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
    40321 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
    40381 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
    40441 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
    40501 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
    40561 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
    40621 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
    40681 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
    40741 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
    40801 gagcttaggt ttataataaa aaaagatgta ggagtggttc aatgaaaaaa gttttagttg
    40861 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
    40921 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
    40981 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
    41041 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    41101 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    41161 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    41221 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    41281 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    41341 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    41401 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    41461 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    41521 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    41581 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    41641 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    41701 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    41761 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    41821 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    41881 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    41941 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    42001 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    42061 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    42121 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    42181 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    42241 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    42301 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    42361 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    42421 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    42481 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    42541 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    42601 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    42661 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    42721 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    42781 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    42841 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    42901 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    42961 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    43021 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    43081 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    43141 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    43201 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    43261 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    43321 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    43381 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    43441 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    43501 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    43561 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    43621 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    43681 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    43741 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    43801 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    43861 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    43921 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    43981 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    44041 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    44101 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    44161 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    44221 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    44281 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    44341 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    44401 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    44461 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    44521 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    44581 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    44641 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    44701 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    44761 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    44821 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    44881 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    44941 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    45001 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    45061 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    45121 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    45181 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    45241 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    45301 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    45361 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    45421 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    45481 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    45541 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    45601 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    45661 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    45721 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    45781 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    45841 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    45901 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    45961 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    46021 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    46081 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    46141 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    46201 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    46261 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    46321 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    46381 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    46441 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    46501 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    46561 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    46621 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    46681 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    46741 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    46801 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    46861 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    46921 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    46981 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    47041 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    47101 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    47161 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    47221 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    47281 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    47341 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    47401 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    47461 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    47521 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    47581 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    47641 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    47701 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    47761 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    47821 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    47881 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    47941 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    48001 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    48061 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    48121 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    48181 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    48241 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    48301 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    48361 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    48421 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    48481 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    48541 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    48601 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    48661 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    48721 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    48781 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    48841 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    48901 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    48961 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    49021 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    49081 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    49141 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    49201 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    49261 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    49321 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    49381 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    49441 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    49501 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    49561 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    49621 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    49681 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    49741 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    49801 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    49861 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    49921 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    49981 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    50041 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    50101 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    50161 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    50221 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    50281 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    50341 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    50401 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    50461 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    50521 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    50581 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    50641 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    50701 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    50761 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    50821 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    50881 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    50941 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    51001 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    51061 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    51121 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    51181 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    51241 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    51301 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    51361 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    51421 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    51481 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    51541 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    51601 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    51661 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    51721 ctggctgccg gaaactttac ttttgttgat agaagaggga ttaatcttat ttgagcgaat
    51781 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    51841 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    51901 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    51961 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    52021 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    52081 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    52141 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    52201 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    52261 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    52321 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    52381 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    52441 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    52501 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    52561 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    52621 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    52681 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    52741 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    52801 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    52861 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    52921 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    52981 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    53041 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    53101 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    53161 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    53221 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    53281 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    53341 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    53401 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    53461 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    53521 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    53581 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    53641 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc
    53701 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    53761 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    53821 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    53881 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    53941 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    54001 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    54061 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    54121 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    54181 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    54241 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    54301 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    54361 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    54421 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    54481 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    54541 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    54601 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt ttttatgacc
    54661 aactggttcc gacggttcca tttgccactt acttccagca catatttatt taggggcaaa
    54721 cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca
    54781 agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt
    54841 aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca
    54901 ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc
    54961 cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa
    55021 ttctatagga agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc
    55081 ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca
    55141 aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc
    55201 gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca
    55261 cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag
    55321 acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga
    55381 agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa
    55441 aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa
    55501 gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc
    55561 taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat
    55621 gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc
    55681 tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc
    55741 taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc
    55801 tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc
    55861 cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac
    55921 tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt
    55981 gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat
    56041 attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta
    56101 gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc
    56161 cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag
    56221 acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa
    56281 gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa
    56341 agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa
    56401 tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc
    56461 atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt
    56521 ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg
    56581 gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc
    56641 gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt
    56701 aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa
    56761 tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca
    56821 agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt
    56881 cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct
    56941 aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga
    57001 gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt
    57061 cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt
    57121 agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa
    57181 gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca
    57241 gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct
    57301 gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac
    57361 tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta
    57421 gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca
    57481 ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt
    57541 tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac
    57601 attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat
    57661 aaatccacat tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta
    57721 tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg
    57781 tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt
    57841 ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg
    57901 taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct
    57961 tgatgttgtt tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt
    58021 tctcaaataa gagaagtgtt ctggtttttt ctacattgca ataaaccaat aaccaatcaa
    58081 aatgatacca aggataatac gataccagcc aaaagcggta aagtcgtttt tcttcaaata
    58141 gcttagtaag aatttgatag ctaagacgga aaccacaaaa gatacgaatg ttcctacaag
    58201 aagaacaaca gtttcttcca ttttgaatga attcccttca gctaaatatt tgacgatctt
    58261 caagccgctt gcgccaaaca tgacaggaat ccctaagaaa aatgaaaact ctgtagcgac
    58321 aaaacgcgaa gcaccaatca agatagatcc taggatcgtt gcgccagaac gggatgttcc
    58381 gggaacaagt gccaagactt ggaataaccc aacaagaaga gctgcttggt acgtaaagtc
    58441 attcagagaa gtccaacgag gtgttttgtt tttgttgcgt ttttcgacaa taataaaggc
    58501 aataccgtaa acgatcaaaa caatggcaac tggtaagaac ttgtggaaat ttgcttctaa
    58561 ccaatcatcc aaaggcaaac caatgatgat ggctggtatg caagcaacga tcactttaaa
    58621 ccaaagtgtc cacgtatctt ttttttctac cgttgattta cttggagaaa atgggtttaa
    58681 cttgtgaaaa taaagaacga cgacagccaa gatcgcccct aattggataa cgacattgaa
    58741 catcgacata aattgtgaac tggcatccag tttgatgaat tcatctgcta ggatcaaatg
    58801 accagtacta ctgatgggca gccattccgt tattccttca ataataccga gaatcactgc
    58861 tttgataata tttgcaaaaa acataaattc cttccttccc aattaagata catcttatag
    58921 tataccgtta ttttttgaga aacccaatct gttttttaat attaagaaaa acttgagaaa
    58981 taaagcagat aagtacacga agagaaaaaa gaagacaaag ccctcgttca gactttgcct
    59041 tttatgattc tctggtaaaa attaaaatat ccccattata gaaacgagtc gcgactatgt
    59101 gttttcccgt tatcgatata agacgtatca ttgatgccgt ggatagaaaa tcgaccgttt
    59161 gcataagtga cctttgttac gctgccattt aaaagcccag cacgaacagg ctgtttagga
    59221 tcgatcagat taagcaaaaa ggcgatcgtc aaaccatgag atacgactaa aacatttcca
    59281 ccaccatttt tttcgcagtg atgggcgata tcttcaaatc ctgtccatac acggtctcgc
    59341 aatgtttcat aagtttccgc ccacccagcg gtatctaagt tgtatacagc attagcaatt
    59401 tgttcaaaag aaacttctgt ggtaaacatt tcatcatatg ttttaaaatt caatacacga
    59461 gggatcacac cccacatttc catgtcatat ccaccttcaa gagacccgaa acaccattct
    59521 ctaatccttt tttcaataaa ataaggaatt tcttttccct tagaatgttc tcctagaata
    59581 atacgggcag tttcgattgc acgaccactg tcactagaat aagcaaatga aaagtcgaca
    59641 ttttttaacc cgcgacctaa ataatgaatg ccttcttgtc cttgctttgt gaggggtgta
    59701 tcacaccagc cttgtactcg ttcaattgta ttgaacatcg gttttccgtg tcgtacgata
    59761 taaagtgtag tttctgtcat tttattctcc tcctaagtcg ttttaacgat tgaaaaaagg
    59821 atttgtcctt ttttcctgtt caatcgtcgt tgcatcgcca tgtcctggat agactgcaaa
    59881 ttcatcaggt aatacaaaca gctgggtccg aatactatga agtaattgtt ctaaattccc
    59941 tgtatacaga tcggttctac cgatacttcc tctaaacagg gcgtcgccag aaacgacaaa
    60001 atcatcaaaa ataaagctga cgcttcctat agaatgccct ggggtaggga cgacgcgaaa
    60061 agacatgttt cctaaagtat aagttttcat ttcaaattca tattctgcag gcgagacaat
    60121 aacatcagca atatcatcat gtctaccgag tccagaaaga ttcaaaatcg gatcacctag
    60181 ccatttttgc tctagtgggc tgacataaac tgggatttga taatacgcgc gtaaaggttc
    60241 cactgcaccg atatgatcgt agtgagtatg ggtaagcaaa atcgctaccg gctgttggtt
    60301 tgtttttttg atttcttgtt tgattcgttc cgcatcttct ccagggtcaa tgatcaaaag
    60361 tgcttcttcg ttgtagacca aataacagtt ttcttcaatc attcctgttt ttaatctttc
    60421 gatatgcatg tgcattcctc cttgatagcc aaaacttctt tcatccatta gtataacgca
    60481 ttcattgcta ggaaacaaaa acaggtactt atttctcttt acttatctga attatcagac
    60541 tatatgaaaa gtatttggat attctaaaga gaaggaaagt aagttttcat agcattttaa
    60601 tatgtccttg ttacattaga ttcatccaaa aatccaaatt ctatatccta tatccctgcc
    60661 taatcagcag ggtgtttttt tatgtttgga aagagagctc tttttgctaa actaggtttt
    60721 ttcaggtaat cttagagaga aagaggtggt ggaaatgatc aaacagtcaa aaatatctgt
    60781 gcggcactta gtagaattta ttttgcgacg tggaagtatc gacaatcgta aaaaaagtaa
    60841 ccatactgca ttagaaggag cgagaatcca ccgcaagctc caaaaagatg cgggagaaaa
    60901 ctatgaaaaa gaagtctttt taaaaacaac agttcaatta gatgaatatt acctgatcgt
    60961 tgaaggccgt gcagacggta ttttcaaaag agacggtatt tattatatag atgaaatcaa
    61021 aacctccgaa ccacgatttg aagatttaga accagaacaa gtggaacttt tctttcatca
    61081 agcaagggtg tatgcctata tctatagtca agaacatgat ttacaagaaa taaatctgca
    61141 attgacttac ttccagacgg cagaagaagt gatcacgaga aaggttgaac atcagacaag
    61201 agagcagtta gaaacttttt ttaaaaagct gactgaagat tataaagaat ggcttgtttt
    61261 tcaagaaaac tggcgcactg tacgcaatgc ctctttgatg gctttgaaat tcccccatga
    61321 tgagtatagg aaaggtcagc gtgaactagc ggtagctgct tataaaacga tccgtacgaa
    61381 acagaaattg tttgtagaag cacctaccgg aacaggaaaa acaatctcta cgttattccc
    61441 cgctttgaaa gctgtagggg aagaagaggg agaaaagatc ttttatctaa cggctaaaac
    61501 aataactaga caagtagctg aagatgctat gacagcatta aaagatacag gagctgaagt
    61561 aaaaagtgtc actttgacgg cgaaagacaa gatctgcttt ttagatgaga cgacctgtaa
    61621 tccagatcag tgcccatatg ccaatggtta ttacaatcgg attaatgaag gtttatggga
    61681 tcttttgaat catgagaatc agatcactcg tgaagtcatc gaaacttatg caagaaagca
    61741 tacactttgt cctttcgaac tatcattaga tgtcagtatc tggtgtgacg taatcattgg
    61801 ggattacaat tatttatttg atccaactgt ttatttaaga cgtttttttg aagatgaaaa
    61861 aaacgaggac tatctgtttt tgattgacga agcacataat ttggtgaatc gttcccgaga
    61921 gatgtattcg gcagaactat cttatgaaaa aacgaaacga agtaaggaag ctataccaaa
    61981 ggaattcaaa aaacttcatc gccgtttcaa caaactatta aaagaatttg attcgatccg
    62041 agaaatagca aaagaagatc attgggatta tcatcatcaa aaagcaccgg ctgaatcttt
    62101 agtaaaggca ggttatcaat taagcgaaaa aattaaagaa tggctggcag aatttcctga
    62161 gcacccatca caagaacaat tgcttcctta ttattttgat ttgcttcatt ttttgaaggt
    62221 cagtgaattt tatgatgatc actacgaaac aacagtcgaa aaaacgtatc gcgatctgat
    62281 agtaaaagag ttttgtattg atccaagctt gtttcttgag cagtcgttgg ataaaggaaa
    62341 aagcagtatt cttttttctg ctagtttttc accactctct tattatcagg aaacactggg
    62401 gggacaaaaa agtctggctt acaaactgcc gagtcctttc ccagaaggaa accagcaagt
    62461 tttcatcgca aattacctag agacgactta tcgaaaaagg gaacacagtc tggcaaaatt
    62521 agtagagacg attcatgctt ttgctgatgg aaaggtcggg aattatttcg ttttctttcc
    62581 ttcttatcaa tatttagatc ttgcggtgga agcattcaga cagcaatatc ccgacagcaa
    62641 tgtgctaatc caagaaacgg acatgaatga ggaagaacgc gaacgatttt taacaaaatt
    62701 tcaaacaaat cccgctgaaa cattgattgg attttgcgta cttggcggga ttttttcaga
    62761 aggaatcgat ttgagagggg accgattgat cggcagtatg atcgtaggtg taggtttgcc
    62821 gcagatgaat catgagcagg aactgatcaa atcatattat gatgagaagg aacaccttgg
    62881 atttgcttac gcttatcagc ttcctggaat gaacaaagtc ttgcaagctg gtggtcgagt
    62941 gattcgtggc atggcggatc aaggaatcgt tgtattggca gatcaacgat tttctagttt
    63001 ttcttatcga cgtttattcc cacaacattg gcaaacagca agagaagtac gaagtgttgg
    63061 ggaattggaa gaaggaatac agcgtttttg gttatcaaaa gaagcagtaa atcaaaaaaa
    63121 cgaggaatga cagacgtcag cctcgttttt tgattaacga tagttagtga attgcaactc
    63181 gattggtaag tcaagtttac gtaataaagc aatcacagct tgtagatcat cacggttctt
    63241 acctgtcact ctaatctgat cttcttggat ttgtgctttt actttcagtt tttcattttt
    63301 gatagctgta gtgattttct tcgcgttttc acgatcgatt ccgttgacca aatcggcagt
    63361 ttgtcgagct ttgccaccta gagcgtgttc tgattcggaa aaatggatat ttttgattgg
    63421 cacatttctt ttgatcagct taccaaataa tacatctttg atctgttcga ttttgaaatc
    63481 atcatcgctc aatacaacta gattgctgtt ctctaatttg atttctactg tagaatcctt
    63541 gaagtcaaag cgattggaca attcttttgt tgtttgctga atcgcatttt ttacttcttc
    63601 tgtattgact tctgatacaa tgtcgaagct agcatctttt gccataggaa ccctcctgat
    63661 atacatataa atatttacca tacatctatc ttatcatagg attagggcag attcttacct
    63721 gtttgctaaa attcttctca tttgagttcg aagtatttta ttttaataga taaactgtta
    63781 ttcttacatt tggaatacca aagtagggga gaggtgcttg tgagtaaaat tatgactgcg
    63841 tatcataaaa atgaaagatt aaagaaaata gtggttattt tgataaccgg attgaccgct
    63901 gcggtcgctt taaattattt tttgattcca gccaatgtct tttctgcagg gatgaatggg
    63961 atcgctcaaa tcatttcctc gcttttatat gaatggtttc atatcaagat agacacagga
    64021 acatttattt ttcttttgaa tatccctgtg tttgtattag ggtttctcaa agtaggaaaa
    64081 cgagcaacga tccttagttt tgtgaatgtt gtatgtgtat cggtgatgac gatgctgctt
    64141 ccagtcggac agatcactga taatattttg atgaatgccc tagtaggcgg tgttttatta
    64201 ggtcttggcg taggattatc tttaaaaatg ggctttacaa ctggtgggat ggatatcatc
    64261 tctcttgtac tttcacaaac aactggaaaa acagtcggaa actacatgct gattctaaat
    64321 ggtatcattg tagtcatcgc aggcttcttc tttaactggg aaagtgcctt gtatacgatt
    64381 atttccattt atgcaatgac tcaagtaatt gatgccatcc acacgagtca tcaaaaagta
    64441 acagcaatga tcgttaccgt caagccagaa gaggtaacag aagaactagc aaaacggatg
    64501 gtacgtggaa tgacattact gccgtctgtc ggtggctatt caaaaaaaca agggaacatg
    64561 atcatgatgg tcatcacccg ctacgaaatg tatgatcttg atcagatcgt tcatgaaatc
    64621 gacgacgatg catttatcaa tattttacca actcattcag tatttggccg gtttgcaaat
    64681 gaaaacgaac aaaaaatgta tcgaacaact ggcgtattgc cagaaataaa aaatcgttca
    64741 caaaaaaaat gaggaatcag gtttatttta gaataaattt aatctattac atctaataag
    64801 aacattttta taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa
    64861 aaaaagagta tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac
    64921 caagaacggt ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa
    64981 atggtgattg atcctgaatt aggaatcgac atcgtcaatt taggcttgat ttatgatata
    65041 gaattcaatc ctgaaaatgg agaaacagtc attaagatga ctctaacaac tatgggctgt
    65101 ccattagcag atattttaac agaatctatc catgacgcat taaaagaagt gccagaagta
    65161 acgaaagcag aagtcaaact agtctggtat ccagcatgga cgacagataa gatgagccgt
    65221 tatgcacgga ttgcgttggg gatacgatag aaatcttggc tgactagcta tgagaagcaa
    65281 ataggaaaaa caaaaaagcc ttttatcctt tctagaagaa aagagataaa aggtttcttt
    65341 gtttataaaa ttttgtctgc tatattataa gttaattaaa taaatgtgtt ctattgataa
    65401 aggacattca aatccctttc cttttggaat ccttcttttt tacatcagtt tgattttcgt
    65461 atagtattct ttctcatctt cacgataaat cgcagcccca tttttttgtt gtgctttaag
    65521 actgccaaga ttttttttat ttacagacaa aagtgcctca tcaatgccga ttttttttgc
    65581 ctcttgtagt actaatgcta atccttgtgt ggcatatcct tttttacgat aattagggga
    65641 gatgccgtaa ccaatatggc cagcgccgtt tcttaatgtt tcgttcagtt tatggcgcaa
    65701 attaaaaatt cctacatact catgttcatc actttctaaa ataaagacgg ttgcgggaac
    65761 gtaactctca ggaagatcga ttcctaaaga cgttttttcg cattttttta cataatctaa
    65821 aaattcagca aatgtatagc cataagcatt gttgataaat ccactctcat ttggtccaaa
    65881 gctagtttgt agctgataag ctttctttaa atctgcttcc catagtttga tcagtttcat
    65941 cgtattcacc tctttaagta accttattca tttgttttct actatagcac tattgttgta
    66001 aatagccatg cctattttta tttattgtgt catatcgtat taagaatcat cgaacaatac
    66061 aaatatgaat tacaataaaa aaattatttg aagttcgttc attcgttact tatgatctct
    66121 agaagaaaga aaacgtgaat tacttcagcg tctagaattc cagaaggata gtaatcaaca
    66181 gaagaaataa tcaatatcca aatgaaagaa gatagctgaa tcgagaaaaa gaggaaaaaa
    66241 gaaatgaaga aaaaaataaa atagttacta atcccagagt cgttcgttta acattgaaat
    66301 cgatcatatg gataaaacag attttttcac caaccctcaa tagatgatgg agttgctcgg
    66361 atgaattatt tgtgtagatg gcataaggga gggcgtacag aaatgaaaaa gaacaaaaat
    66421 atgttcttga caaaaaacac atgtaatcct aaactaaaag atgtgatgcg gctatggcgg
    66481 aattggcaga cgcgctagct tcaggcgcta gtgagggtga cctcgtggaa gttcgagtct
    66541 tcttagccgc ataatttgtt tttttataaa gagaaggtac acctggagcg atgagaaaga
    66601 aaataacata ttatctaaaa gaatatagga aaaattttaa acaacggaac cagattcaaa
    66661 taaatcaaaa aaaatttgtg tgaaggtggg acagtttaaa acctaataga ttgattcaaa
    66721 tagtagattt atagattctg atacaagaaa tactggcaaa cgatagttaa ggaataaaga
    66781 ctaaacttaa actcaaaaag aggtcatgcg aaagaataaa tcgcatgacc tcttactata
    66841 ttttgaatgt gtttaaacgt tcaaataatg aatcttagta ccaaccgttt gcttcccaga
    66901 atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt
    66961 cttggtttgc agcagagtag tcaccattta agtaagaaga atctaattgg taacggccga
    67021 tgtaacgtcc gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt
    67081 ttgcagagct tgtacttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg
    67141 tttcagcaac aggcgctgct tgtgtttccg tttgaaccgt ttcttgtaca acaggttgtt
    67201 cagcttgaac aggttcagct tggactggtg cttcttgaac ggctgcttga ggttcagcag
    67261 ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagtag atcaggttaa
    67321 tatcagaaat tgaatttgat tctgcaatgg cattgatcaa ggagttatca ccaacgtatt
    67381 tttgagaaat cgttgataga gtatcacctg attgaacggt ataagcttcg tccgcatgag
    67441 cagtcgttcc catgaagaat gcagcgccgg cagccaaagt tgttccaaaa agtaaagttt
    67501 taagtgatgt catgaaaaat ctcctttatt cgttaaaaag ttttatttgt tgtgttcaac
    67561 gaggaatact ttaacacgtg aaaatatttc ataggtaaaa gaagcatgac aatttatgac
    67621 aaatccacgt tttttgtaac agaatgatgt tcgctcgatg tgaaaaatga tgaggaattt
    67681 cacataatgc agaaataatg ggattagaac aggtgttctg atgagaaaag aaatgtataa
    67741 ataacttgaa agaaattgct tttgttcatc attttattta catattgcgt atattttttg
    67801 tatttttgct tgagatgtta caataaaaga aaaaggaggc ggttccaatg agtaaaagat
    67861 accagaacat catggtagca gttgacggat caagacaatc gatcaaagcg ttcgctgaag
    67921 cccttgactt agcaaaggac aatgaagccc acttatttat cgtgtcaatc atcaacaaag
    67981 ttgagctgac ccatagtgcc tatgcctttt caaaaatcta tgcagatgaa aaacaaagaa
    68041 cagaagtagc catgctgaaa aaaatcaatg atgccaaaga gtacggcatt gctgagatcc
    68101 acgcaatagt tgaaactgga gatccaagaa atttgattgc caatgtcatc cctggacaag
    68161 aaaatattga tttgatcgtt atgggagcga caggaaaagg tgctatacaa caagcattag
    68221 ttggatcgac cgcttcttat gtcgtatcac atgccccttg cagtgtgttg gtcgtgaagt
    68281 aatgagataa gggaggaata gaaaatggtt cgtcctcaag aagtccgtgc gccaaaagaa
    68341 aaaatagaaa ttttagcaat tattgaagac ggcacacaaa caaagaaggg atactcgatc
    68401 gctttagtaa agtggaaagg aaaaaaaggt gttgctctga gatgggatgg agataaccaa
    68461 caagataaag gattccctgt cacagctaac ggttatcatc cagcgtggtt tgttctgcct
    68521 gataaattta tggaattata cagctatgat tatgcaagag ccgcagcatc aatcaaagca
    68581 ttagataaat taacagaaga gcagaatgaa atggaggaaa aataaaaaag acgaaccaat
    68641 cagcctttag aaaactgatt agttcgagga gtaaaaatga aaaaaggtta tttgggatga
    68701 aactagaata atgcgtataa cttaaataaa acttaaagca ataaaatggc aagagggtga
    68761 ggcaaatgga agcaggaaaa gtattacagg aagtaaaagc actatgtgca gataaaaaat
    68821 atgaagaagc gaaaagcctg atcgaaaaga acaaagaaaa tttggaagaa aagtacccga
    68881 tcgcacaaga atttatagaa ttaaaacaag gaacgatcct tcaccgattc aaacattttt
    68941 ttggtataac agaatagaaa aaacgattaa agatcggcag aaatttgctg catctttaat
    69001 cgttttcttt ttaaacatgc gagggattca attctgattc ttatataaaa aatagatcca
    69061 aataataaga aaaattactg aaagcgtcaa ttcgatgatg tgtgttatta atacagacgt
    69121 tgtttcccaa tgcctaaaag aaaaaatttg ataagtaaaa tatacagcag gtaaagaggc
    69181 cagtaaaact gaaaacttct tttttgtcca ttttatgtaa tagaaaagaa tgcttatcat
    69241 aataaaaaaa ggaatataaa aaacaaattg accgatcgcg atcatactat tgcctccgat
    69301 aaataaagtc catgatacaa aataacactt tgtgcgaaat ggcgcaagca gttttgtgga
    69361 tttatccggt aaaatctgta tcgattttga aattagagga gaaacttgct ataatcaaag
    69421 tgaaagaagg agtgggattt tcatggatca tgtagtgtta gaaatcgatt tgcatttgat
    69481 caataatatt cgggtgattt actatgttgt aagtaacagt gtcgaacaac gtgtactgac
    69541 gaataaaata aatggtattt tagcaaagaa agacgttcac cgattcaata atggtgaagg
    69601 aagttattat agtatcccag tagaaaaaat tatttatacg acagtcaaag tcagagaaga
    69661 tctagatacc aaaaaagcat atgaacctat ttttacaact tattgattga cagcagatgg
    69721 aggaaaaaaa tgaatccata tgaacaactt attcaagatt tgatcgatgg aaaaatcgaa
    69781 aagattgatg taaaaagaga agaattaatg gcttttcgag aagcgtggct gaagttggag
    69841 aatcgtaaat atgtggtggg aaaagcctca ctgaacggca atgtgactta tcattacgat
    69901 ccgacacgtt tattttagta tgaagtattc agaaaagaac caaaggagga cgatgcagca
    69961 gcattgtcct cctttgattt tacaaaagtg aaagttttac ttatttgaat agatttttaa
    70021 atttgttttt gacactgtcg atatttacat cttttaaaag attttgggct ttctctttat
    70081 aaggtcctaa ttgttcttta tgttcttcga taaatcgttt tgcgccatcg aaattgccgt
    70141 tcttaatcat ttccttgact ttttgaaata attcttcttg attcattttg aacacatcct
    70201 ttgctatcaa catgattttt tacatggaca aaacgagaaa aactgattta tccacacgtc
    70261 aataatatca aataacgatc atttttacta aaaaaacact cgattctcgc ttctaaagta
    70321 ggagaaaaat ctccaacttg ggacggatgg aatttcttta actgacccat atactgaata
    70381 tataacaagg aggtagaaag atgaaagtga ttaaatattt gattggactg gcggcgatta
    70441 gtggtatttt gctatttgga ctgaaatatt atactgaagg aagttatgga gaaatccctg
    70501 gtatacttga tcagctaaat ccattagttg aaaaaggaga ggtctatgta aagacacaaa
    70561 aaccagagga agtaaatgaa tatggtacag caaggtatgt gcagaaagct gctgatgcca
    70621 atgggaaaga acgaacggtt gaatttaatg gtctttctgt gttaaaagaa aatcattatt
    70681 tgaaaattag caataaagga gcacacatag aaacgtatga ggaagttcct agagaaaaag
    70741 ttccggagaa agcattagca attattgggt aaatcagttt ttgtattaag aatacgcccg
    70801 attttattat tcataattta attatgtaaa ctaaatacaa gtagtaaaaa taatacaata
    70861 atccccctgc aaaaagattg ttcttttaca gggggttcat tttttcagca agcacatcaa
    70921 attgggatgg cagggttcga acctacaatt gcttgattca gagtcaagtg ccttaccagt
    70981 ttggctacat cccatttatt caacaaaata atcatagcac tttcctaaaa aaacgcaagt
    71041 tatttttctt gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac tagtttcttt
    71101 tctaatccag caggaagggc aatcagttct gctactgaca agacggtgtc caataactga
    71161 tattctttga caggttcatt cataatatga tagatgtttg ctaaacgttc aatatattca
    71221 aagcgagtat actctgttcg agcaattaga aatttcaata aatcggtggc tttctcgtac
    71281 tcttgatctg cgtaataaac aagagattga cgatacaatt tgatttccag ttcattgagg
    71341 gtagcaggtc tgcgacgctc gttccactca ttaatagaga ctttaccttg aacatttccc
    71401 ttgcagtcac ggacaaaaat aatcggaatt tcatttggat ccattttttt atgtgtgaat
    71461 ttttccatta tctacgctcc cttcaaaggt ttggtatctt gagtataagg gatttttgtt
    71521 tgaattacta gcttttctcc aaagttagca tctgtccacc cattgcagtg aaaatcgccg
    71581 aaaaatcgtt gatcggtact gctcgatttt ttacaccata gggatcgttg acataaatat
    71641 tttctttgtc atatccagtg actacacaag catgaacaag aggagtgact cttacttctc
    71701 cattatttgt ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa ttgatagtca
    71761 cagaagtcca gacaggattt cctttagata gtgctttttt gatatcatcg agttgagcat
    71821 cggtttttgg aaaagtgtga taatccgttt ggacgatttg ctgagcaagg gaggcaactg
    71881 ggggaacata gacgaccata gcccagtctc cgccactgat atcaccaaca aatccttcat
    71941 taggatcgcc atgagtccca tctgcattga aaacagggac gtaatccaat tgctcagcta
    72001 actggttttt agttgtttca taaccataaa aagataaaag catagaaagc gaagtgactt
    72061 cacagccatt ttctaaagaa ggagaatcaa actggctctc taatggaaca tctagtaaaa
    72121 cttctgcttt ctcttttgtc tttttcgttt tttgtgttga acttttttgt gcagtgttca
    72181 ttgtatgttc tggagaatgg atatgtaggt aataacttgt accgcttaat aaaagagcag
    72241 ctgtgccccc tagaaacaat agttttttga acgttttttt cttcatttga aatctccctt
    72301 ttcagctgtt tatctttcct attataaatg tatttttatt ggaaatggaa gtattatatt
    72361 ttaatatgta ggaaataatt agtttttaag gaatatgttt taattttttg aaaaaaattc
    72421 ttctaaatac aacagctgtt ttaattgtga aaacgaaaac agtacgctat aatgcctgta
    72481 aggttgattt ttaactgtaa ataaaaatca atgagttaaa tctccaaaga aatagcagtg
    72541 gtaatgtgga gaaaagttcc agcagtttag gaggattttg atgacggaag aaatgattaa
    72601 tttaggagaa caatatgcat gcaaaccaat tggttttaca aaaacggtca ttggtgaagt
    72661 tgtatcaaaa atgacaaact gcgccgtggt caaagttgcc caatgtgctg cagaggacca
    72721 agaattatta gatgaaaaag caagcatggt tgttgcaaaa tatgatacat ttgaataaag
    72781 aaaacaattg gacgtgcggc gtgctgcaag tccaatttct ttgagaaggt aaatcagtat
    72841 tcttttccta ttttctggag aagaaaaagc gaggcgagtt gtcatggtaa cattctttac
    72901 ggttctattg tttttattcg ttgttgtgat gtttttattt ctttggctgg ttcgaaaaga
    72961 aattatttat cggactgtgc gaaatcgatg ggtctacttg gtgataccct tcttagtgtc
    73021 actagtcatt tggtatacac tcatcagtca tcctacagca gatgaattgg caaaagggat
    73081 cttgtcagca atgattttta ttagtttttt gttggacagt agaggaatca cagaagaagg
    73141 acttgttttg aatagctttg acaaaaaagg tgttcctttt tctgagatca ataaaattgt
    73201 attatatcag ccaaaaggtt ctaaaatcgt aaaaatgaat tttttccgaa acggatggcg
    73261 aggaccgatg ctgaaatttt ctgcatcatt agaagaatta gttccatttt tgtcccaacg
    73321 cttaaacgat gaggcagaga ttgatattat gattgatcca gaataaaaac agaggttgcg
    73381 acaagatgtt tgtcacaacc tctgtttttt atttcaaata aaattatcca aatacgcgga
    73441 agcgatcggc atcgttcatg tattcttttt ccccagccgg tgtttctggt gaaccgaata
    73501 ctaattgcga acgtagtttc cagtttgaag ggatattcca ttcttttgca actgcttcgt
    73561 caataactgg gttgtaatgt tgcaagtttg cgcccaatcc tttatcaact aatgctaccc
    73621 aagtattagc tgttgcgatt ccgtttgatt gttctgacca atctgggaag ttgtctgcat
    73681 ataattcaaa ttgttcttgt agacctttta ccacgtcagt atctttgaag aataatactg
    73741 taccataacc atttttgaaa ccagctaatt tgttttgtgt atttgggaaa gcttctgctg
    73801 gagtcaatgg gcgaagtgct tcttcagtga tttcccaaag tttttcatgt gcatctccga
    73861 aaaggataac tgcgcgtgtt gattgagcat tgaaagcagt tgggctttct ttaattgctt
    73921 ctttgatcaa agttgttaat tcttcattag aaagtgaaac attacgtcct agatgataga
    73981 ttgaacggcg gtttttcaaa gtgtcagtaa aagttgtcat aataaaattt ctcctttgat
    74041 ttgcgttttg cattttttta attgattacg aaatgaagta tagcacctta caaaaagtaa
    74101 gtaaacagat atgctcacaa aaaataagcg aagttcaaaa aacaatttta ataagaaaaa
    74161 tcgttatgta aggattttct tagttatcgc tttcattgtt gtgttttagg aatttttgct
    74221 tatttgaaaa aaacagctga tatataatta ttaaatctct atcttctttt ttttatgata
    74281 ttgccattaa aaattacttg cttttataga aaattagttt atatttctta aat
//
LOCUS       DACEJR010000007        68872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEJR010000007 DACEJR010000000
VERSION     DACEJR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.007"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW651_000487"
     CDS             414..947
                     /locus_tag="IW651_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0813242.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="IW651_000488"
     CDS             complement(1437..1961)
                     /locus_tag="IW651_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813243.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="IW651_000489"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="IW651_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0813244.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="IW651_000490"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="IW651_000490"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0813245.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="IW651_000491"
     CDS             complement(3683..4363)
                     /locus_tag="IW651_000491"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0813246.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="IW651_000492"
     CDS             4489..5358
                     /locus_tag="IW651_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0813247.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="IW651_000493"
     CDS             5384..5899
                     /locus_tag="IW651_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813248.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="IW651_000494"
     CDS             complement(5946..6509)
                     /locus_tag="IW651_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813249.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="IW651_000495"
     CDS             complement(6564..6743)
                     /locus_tag="IW651_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813250.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="IW651_000496"
     CDS             complement(6820..7329)
                     /locus_tag="IW651_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813251.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="IW651_000497"
     CDS             complement(7425..7805)
                     /locus_tag="IW651_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0813252.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /locus_tag="IW651_000498"
     CDS             8135..8884
                     /locus_tag="IW651_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0813253.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="IW651_000499"
     CDS             complement(9068..10345)
                     /locus_tag="IW651_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813254.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="IW651_000500"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="IW651_000500"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0813255.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="IW651_000501"
     CDS             complement(11267..12211)
                     /locus_tag="IW651_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0813256.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="IW651_000502"
     CDS             complement(12213..12899)
                     /locus_tag="IW651_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0813257.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13373..13693
                     /locus_tag="IW651_000503"
     CDS             13373..13693
                     /locus_tag="IW651_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0813258.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            13847..14752
                     /locus_tag="IW651_000504"
     CDS             13847..14752
                     /locus_tag="IW651_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0813259.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14749..15522
                     /locus_tag="IW651_000505"
     CDS             14749..15522
                     /locus_tag="IW651_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0813260.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15703..17127)
                     /locus_tag="IW651_000506"
     CDS             complement(15703..17127)
                     /locus_tag="IW651_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0813261.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17338..17784
                     /gene="nrdI"
                     /locus_tag="IW651_000507"
     CDS             17338..17784
                     /gene="nrdI"
                     /locus_tag="IW651_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0813262.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(17849..19831)
                     /locus_tag="IW651_000508"
     CDS             complement(17849..19831)
                     /locus_tag="IW651_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0813263.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(19845..20741)
                     /gene="murQ"
                     /locus_tag="IW651_000509"
     CDS             complement(19845..20741)
                     /gene="murQ"
                     /locus_tag="IW651_000509"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0813264.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(20744..21805)
                     /locus_tag="IW651_000510"
     CDS             complement(20744..21805)
                     /locus_tag="IW651_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0813265.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22016..22543)
                     /locus_tag="IW651_000511"
     CDS             complement(22016..22543)
                     /locus_tag="IW651_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0813266.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(22610..24112)
                     /locus_tag="IW651_000512"
     CDS             complement(22610..24112)
                     /locus_tag="IW651_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0813267.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(24370..25491)
                     /locus_tag="IW651_000513"
     CDS             complement(24370..25491)
                     /locus_tag="IW651_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0813268.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25617..26558
                     /locus_tag="IW651_000514"
     CDS             25617..26558
                     /locus_tag="IW651_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0813269.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26708..27604)
                     /locus_tag="IW651_000515"
     CDS             complement(26708..27604)
                     /locus_tag="IW651_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0813270.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            27873..29213
                     /locus_tag="IW651_000516"
     CDS             27873..29213
                     /locus_tag="IW651_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0813271.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            29431..29874
                     /locus_tag="IW651_000517"
     CDS             29431..29874
                     /locus_tag="IW651_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0813272.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(30378..31796)
                     /gene="nhaC"
                     /locus_tag="IW651_000518"
     CDS             complement(30378..31796)
                     /gene="nhaC"
                     /locus_tag="IW651_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0813273.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(32199..33422)
                     /locus_tag="IW651_000519"
     CDS             complement(32199..33422)
                     /locus_tag="IW651_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0813274.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(33453..34292)
                     /locus_tag="IW651_000520"
     CDS             complement(33453..34292)
                     /locus_tag="IW651_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0813275.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(34309..34989)
                     /locus_tag="IW651_000521"
     CDS             complement(34309..34989)
                     /locus_tag="IW651_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813276.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(34991..36064)
                     /locus_tag="IW651_000522"
     CDS             complement(34991..36064)
                     /locus_tag="IW651_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0813277.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(36711..37382)
                     /locus_tag="IW651_000523"
     CDS             complement(36711..37382)
                     /locus_tag="IW651_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0813278.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(37469..40147)
                     /gene="mgtA"
                     /locus_tag="IW651_000524"
     CDS             complement(37469..40147)
                     /gene="mgtA"
                     /locus_tag="IW651_000524"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0813279.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            40806..41243
                     /gene="ndk"
                     /locus_tag="IW651_000525"
     CDS             40806..41243
                     /gene="ndk"
                     /locus_tag="IW651_000525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0813280.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(41265..42131)
                     /locus_tag="IW651_000526"
     CDS             complement(41265..42131)
                     /locus_tag="IW651_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0813281.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(42291..44384)
                     /locus_tag="IW651_000527"
     CDS             complement(42291..44384)
                     /locus_tag="IW651_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0813282.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(45133..45741)
                     /locus_tag="IW651_000528"
     CDS             complement(45133..45741)
                     /locus_tag="IW651_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813283.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(45900..47297)
                     /locus_tag="IW651_000529"
     CDS             complement(45900..47297)
                     /locus_tag="IW651_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0813284.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(47329..48300)
                     /locus_tag="IW651_000530"
     CDS             complement(47329..48300)
                     /locus_tag="IW651_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0813285.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            48674..49099
                     /locus_tag="IW651_000531"
     CDS             48674..49099
                     /locus_tag="IW651_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813286.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(49587..50288)
                     /locus_tag="IW651_000532"
     CDS             complement(49587..50288)
                     /locus_tag="IW651_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0813287.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(50304..50744)
                     /locus_tag="IW651_000533"
     CDS             complement(50304..50744)
                     /locus_tag="IW651_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0813288.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(50744..51319)
                     /locus_tag="IW651_000534"
     CDS             complement(50744..51319)
                     /locus_tag="IW651_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0813289.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(52167..52646)
                     /locus_tag="IW651_000535"
                     /pseudo
     CDS             complement(52167..52646)
                     /locus_tag="IW651_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(52805..53506)
                     /locus_tag="IW651_000536"
     CDS             complement(52805..53506)
                     /locus_tag="IW651_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0813290.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(53614..55023)
                     /locus_tag="IW651_000537"
     CDS             complement(53614..55023)
                     /locus_tag="IW651_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0813291.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(55071..56936)
                     /locus_tag="IW651_000538"
     CDS             complement(55071..56936)
                     /locus_tag="IW651_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0813292.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(57057..57908)
                     /locus_tag="IW651_000539"
     CDS             complement(57057..57908)
                     /locus_tag="IW651_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0813293.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(58218..58931)
                     /locus_tag="IW651_000540"
     CDS             complement(58218..58931)
                     /locus_tag="IW651_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0813294.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(58999..59325)
                     /locus_tag="IW651_000541"
     CDS             complement(58999..59325)
                     /locus_tag="IW651_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0813295.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(59448..59675)
                     /locus_tag="IW651_000542"
     CDS             complement(59448..59675)
                     /locus_tag="IW651_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0813296.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(59997..61250)
                     /locus_tag="IW651_000543"
     CDS             complement(59997..61250)
                     /locus_tag="IW651_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0813297.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            61311..62051
                     /locus_tag="IW651_000544"
     CDS             61311..62051
                     /locus_tag="IW651_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0813298.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(62137..62895)
                     /locus_tag="IW651_000545"
     CDS             complement(62137..62895)
                     /locus_tag="IW651_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0813299.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(62892..63920)
                     /locus_tag="IW651_000546"
                     /pseudo
     CDS             complement(62892..63920)
                     /locus_tag="IW651_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(63940..67107)
                     /locus_tag="IW651_000547"
     CDS             complement(63940..67107)
                     /locus_tag="IW651_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813300.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(67331..68215)
                     /locus_tag="IW651_000548"
     CDS             complement(67331..68215)
                     /locus_tag="IW651_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0813301.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(68310..68747)
                     /locus_tag="IW651_000549"
     CDS             complement(68310..68747)
                     /locus_tag="IW651_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813302.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        19828 a        14460 c        11917 g        22667 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatga cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata
    12961 tagttgtttt ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttatcac
    13021 aaaaattatc ttacttattg taaggtaaag ctttttttaa tttattgccc gataatatat
    13081 agaaataagg tctagttact gtttttttta aatgattaat aaataacaac ttctttctat
    13141 gaaattgcaa agcattcatc ttcttttccg ctaacgatat atccgtcagt gcttggtaca
    13201 ctttactaaa aagtatgaga ttgccgatat ctggataggt gtgctatact acagaaaaag
    13261 atatacaatc tgccaccggg taggataaag aggatgatca ttccattagg tctttgaagg
    13321 gatgattttc ctctttttgt gtattttttt atagttaagg aggaatgtaa gtatggcatg
    13381 gatggaatta atcatagcag gaattttgga agtattctgg tcaacgatga tgaaatggag
    13441 tgatggtttt tctaaaatca attattcttt ttacacagtg attggcatga ttgctagttt
    13501 ttactttttg tcaaaggcaa tcaaaagttt gccgatgagt ctctcttatc ctatctggac
    13561 agggattggc gcagttggtt cggtcataat tggtgtggtc ttgtttcatg ataagctgaa
    13621 tctttccact tggttctttg tggggatgct actcatcagt atcattggca tcaagattac
    13681 tagcggccac tagacacttg gaataagtac tgatcagaaa aaataagcct ttttatttaa
    13741 tattcaattt ttttatgaaa aaataagaaa taagtccaat gacctagcga cttctccact
    13801 catcaggtgt atgcttgtac tatctataga aaaggagtga atatttttga aaagtctttc
    13861 ggtccaagaa ttatccaaga actttggtca caaacaagcc ctcagcgatg tcagttttag
    13921 cttatcccct ggcaaaatcg ttggattagt aggtcctaat ggcgctggaa aaacaacgat
    13981 catgaaagct attttaggac tgatccatta ctcagatgga acaatctgta ttggtgaaaa
    14041 tgagatttct ccctctagtc ataaagggtt ggaacaagta ggagcattga tcgaataccc
    14101 gggtatctat ccattcttaa caggctatga tcatctgaag ttattctctg aaaccaacga
    14161 tgtatctgct atcgacacta tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa
    14221 agcgaaatcc tactcgttgg gaatgaaaca aaaattggga attgctcttg ccttactgaa
    14281 ccatccgtct atcgttattt tagatgagcc gatgaacggt ttggatccac aagcaacaag
    14341 agatgtgcga aaaatcatta ccgacttggc tactcagggc acctctttct tgatctccag
    14401 tcacttactc agcgagcttg aaaaaatggt cgatgagctg atcctgatca atcaaggaaa
    14461 gatcatcaaa caatgtacaa tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat
    14521 ctctacaaca aatgatctag ctgctcgaac gatcttgaca gaagctggat atactttgga
    14581 agatcctgat aaaatcaagc ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca
    14641 gctattgtct gcacaaaata tccatgtgac agacgtccag catctgacga atgatttgga
    14701 atcttctcta ttggaaattc tggattcagt aaagggggaa gaagaaaaat gacattgctt
    14761 gtaaaacaag aattatttaa attgatcaag aaaaagtcaa ctgctgtttt atctgttcta
    14821 ttagttgttt tgctcatagg aacagcactt ctagccaaaa aatacacgac gatcattgat
    14881 ccagttgaaa tgactgctca gctttttagt gccacatctt ggattgtctt tatcatgatt
    14941 gcagctgcaa gcacgatcat ttcgatggaa gcacaatatg gtacattgaa aaatctcctt
    15001 tatcgaaagt attctcgagg agaaatatta gtaagcaaat ggatcacatt agttatttat
    15061 tctgtttatt tatacctttt agctattatt gtcactgtgt tgatgaaact gattcttttc
    15121 ccttcgattt ccttcacgga gaaggtttct actggtcaaa cactgatcca aagcttgttc
    15181 acttatactt tgggtagtta tatcggttta tggctgattt taagtttagt gttgatgatt
    15241 gcttgcttta tcaatagttc cggcgcatcc atctcagcag gaatcgtctt ttattttgct
    15301 tcgtcaatca tatctggtat ccttattgca ctcatccaaa aatgggagtg gatcaaatgg
    15361 aatccgatca gtatgttgaa tctgcaaaac caaattggaa acgaagagat catgaagcaa
    15421 ttgactcatc tttctactaa tcaaatgcta ttcggtaatt tagcttacat cgttttgttc
    15481 ttagcattag gatatctcgt atttaagcga aaaaatgtat gatgactcat caaaaaagag
    15541 gctgtgaaag agacgttcag ccttattgcc gattggctgt ctcttaaaca ccgtttattc
    15601 tgaaaaagga gctgtgacaa catttgtgtc acagctcctt tcctagtatg tttttgaata
    15661 aacgacaggc tcacaacctc tctttatact tcttttatac ttctatacgt gaagcatccc
    15721 agtggcaaat aaaaccattg caatcactcc gaccatcgtc acacctataa gtaaaatctt
    15781 atctaagtgg atatgttgta caggctcgtt ttgctcctta cgtgctttga ggtaaaaata
    15841 aatgcctggc aagaaaagaa caagaacaag cattacttca ttaaaggcta cgactagcat
    15901 agcccatact tgaaaggcac ttgctaaaag ccctactaaa aattggaccc attctttccg
    15961 ctgataagaa agtttcattt ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc
    16021 tgtaccaagt gtgtaagcaa agttataagc actgtcagtg aataataatg aaagtaaaaa
    16081 gacattcgta cataacgtcg tcaatacgat agcataagtt ggtgccccat ttttgttgag
    16141 ttttccccat gctttaggca aaagtccgtc ttcagccatg tttcccattg tttcaatcgg
    16201 taagatcgtc catgagagcc aaacaccaac taaagaaatg atcaaaccgc cgttgatcac
    16261 gactgctccc catttaccaa ccatcatttc aaacacatag cccatagcag gctgcgcctt
    16321 gatcgtttgc agctgattca tcgtcattgt cccatatgga agcatggaaa ccaagacgta
    16381 aataataatc aagccgaata tccctagaat cgttgctttc ccaacatctg attgtttttt
    16441 tgctcgtgaa cctacgacag aggctccttc aataccaaca aatacccaaa gcatcaccat
    16501 gatgcaactc aatacttgac tggaaaacgg gatcggctgt ccattcgcat tcaatataat
    16561 gtcgctagaa aagttatcaa tgaattgttg gatcgaaaac gtgctgatag ctactacaat
    16621 aaataaaaat aatggcacta atttacagaa tgtgacgata gcattgacga aactagcatt
    16681 ttcgactccc cgattcacta gccatgcata tccccataaa aagactgtac tcatcacgat
    16741 cgatgccaga ttttgcccac cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa
    16801 caatgttgca aaagctacgt tgcctaacca gcatgtgagc cagtaacccc aaccgctgat
    16861 aaaaccagcg aaacggccaa aaccttcttc tgcgtaacta aagatcccgc cttttaaatc
    16921 tggacgtttt ttattgatat tattgatcga caaggagagc atcaacattg ctgttcctac
    16981 gagtacccaa ctgaaaatag ctgggccgcc agccgatccg gagatgtccg tcataatacc
    17041 aaatacgcca cctccaatcg ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat
    17101 tccttttttc tgttgctctg tatccatgta agcatcctcc aaaataaaac cgcttttatc
    17161 aaaaagtata acaaaccttt tttattttta cttaattaat aaaaacttta atagaaatat
    17221 aattctaccg gcttttttct cattatacaa gcaaagcgcc tagtcccttt ccaaattaca
    17281 ccaccaaaaa acttaaaaaa gatgctatac taaaaggaaa gaattggagg atgatccatg
    17341 aacattttat atatatcgat ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa
    17401 tatgctgaaa aaatgcatgc agaagatcct gtcaatcctg aagttacttt aaaagaaatc
    17461 catgaaaata gtgattttac gaaagaaaca gaaccatttt ttactttcgt tcctacttat
    17521 ttagacggcg gaaatggttt agataacggt gatacagaaa tcttgaccga aacgatgcgt
    17581 gaatatctgg aacatgaaga caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat
    17641 aaaaacttca ataatcagta ttgtctaaca gccaaacaat atgcacaaac atttggcttt
    17701 cctttcttgg cagattatga attacgggga acaccatctg atgtgacacg aatttatcag
    17761 attttagcag aaaacaataa ataataaaaa agaagaagct gaaaaataga tttcagtttc
    17821 ttctttcgtt tctattgttt ctatcatatt aagctttgac atccatcaca ggtgcttttg
    17881 cttttgcttt acctgttttt tccaagacaa agttttttac acgatccata ttggttacca
    17941 agacgatcat ggatgtttct ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta
    18001 caagcgtatc aggtgtcact tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc
    18061 ctaagtcaac tgtgttgatt cccatatgaa gcaagatttc taaaccattt ggcgttgtga
    18121 tcccaatagc gtgtttcgtt gggaagacag agctgactac cccagtaact ggagaataga
    18181 tatgtccgtt ttctggttct acaaagtagc cttcacccat catcttttgt gcaaaaactg
    18241 gatcacttgc tttatctaaa gaaacaactg ttccgtcagc aactgatgag aggatagtca
    18301 cactttcagc tgaagtttga ggtactactt tttcaggcac ttcatttact gtggctgttt
    18361 cagtcgctaa ttcttctttt tctaaacggt ctttaggaat accgaagaag aatgtagcaa
    18421 cgaagccacc agcataagct gccaataatc caaggatgta gcctaaccat ttaccgtcag
    18481 agatcaatgg gatcaaagcg gctcctgaag gtccaatagc gatagccccg acatttccga
    18541 tcattccgat cactgccccg ccgatacctc caccgataca agcagtaata aatgggcgtc
    18601 ctagaggtaa tgttactccg taaatcaatg gttcgccgat tcctaggatc ccgactggta
    18661 aagctccttt gatcatttct gttaattctt tatctttttt gcagcgaatc cataatgcca
    18721 atgctgcacc cacttgtcct gcaccagcca tcgcaagaat tggaagcaat agcgtacgac
    18781 cagtttcatc aatcatttgc acatggattg gagtcaaaat ttgatgcagt ccgaacataa
    18841 ccatcggcaa gaataatgtg cctagaacga agccggcaaa tgctccacca acgccaagta
    18901 cccagttgat cccaccgacc atgccatcag aaatgaaacc tgctaaaggc atgatgaaga
    18961 aaatttcaat cagaccgatg gcaatcaatg aaagagttgg tgtaacgatg atatctaccg
    19021 catcaggtac tactttatgc agttttttct caacgattga caatagccaa acagcgaaga
    19081 tgacacctag gatcccacct tgtccagctg caagagcttc accagtaaat aagtttttca
    19141 gtggtgcttc cggattcatc cctgtcagca tcacaacggc cccgataact ccaccaagag
    19201 ttggtgttgc accaaaaact tgtgcagcat tgatacctgt atagataact aggtagctaa
    19261 acatcccgtt tttcaagatg ttcatcacat cgatatattg ttgccatgtt gctgcatcaa
    19321 gtgttccggc tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg atcccagtac
    19381 cgacaaatgc aggaatcatt ggcacaaaga tattagaaat gtcttttaat accgatttga
    19441 ttttagatgg tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta
    19501 cacggtcttt tccagatgct gatgaatttg ctgtagctgt acttacagaa gaaagttttt
    19561 ctcctaattt cacgcctgcc aaatcaacca tttgctgtgc tactttgttc actttcccag
    19621 gtccgatgat gacttgaagc tgttcatctt ctactacacc aagcactcct ggaatcgctt
    19681 ttaattcaga gacatttaca agatcatcat tgatgatctt catacgcaca cgagtcatgc
    19741 agtggacaat gctttctaca ttgcccatgc cgcctgcccc tttataaatt tgttttgcca
    19801 gttcttccaa agtcagttcg ttttttgcca tggtcttttc cccctcataa ggtccctttt
    19861 acaaatccgt ttgcttcagc taattttgct tgagcttcct ctttatcact atttgtcaaa
    19921 atcatgacga tagccagctt aacattttga tctgcttctt caaagtactt ttcagctgtt
    19981 tcgtaatcag cttctgtcgc ttgcatgatg attcgttttg agcgttcgat tagtttttca
    20041 tttgttggtt tgacatcgac catcaagtta ttgaatactt tacctatacc aatcatactg
    20101 atagtagaaa tcatattcaa gataagtttt tgtgctgttc ctgacttcaa tcgagtagag
    20161 cctgtcaaaa attctggacc tgcatcgact tcgattggga aatccgcgtg tttactgatt
    20221 tcggcttgtt tattgcaaga aatcgttcct gtgacagcac cgatcgattt tgcgtaatct
    20281 aatccaccga tcacatatgg tgtacgaccg ctagctgcga taccaataac tatatcattt
    20341 tttgtcaaat tcagcttgat tagatcttct tgccctagct cataagaatc ttcggcccct
    20401 tcgacagcta cagtcattgc ttcttctcca ccagcaatta gacctatgac catttccggt
    20461 tctacaccga atgtcggtac gcattcagca gcatctaaca cacctagtct tccacttgtt
    20521 ccggctccca tataaatcag acgtccacct tttttaaagg actcaatcgt gttagcaatc
    20581 actggttcga tacttgtcag ttctttttca accgcagcgg ccactttctg atcttctttg
    20641 ttcattagtt gaacggcttg agaaatactc atctcatcta atccaaaagt ttcttggttt
    20701 ctgcgttccg ttgttaggtt ctctaaattg attttgctca tttttatctt ccttcttttc
    20761 ttataaattg atagttcata ccagctttga tttggttgat caatggtagc tcttctttga
    20821 taactttagc tactacattc actttttcat ccgctggcaa atcatatttg gttaattgga
    20881 tctcacccat atagcgcaaa tacttttcgt tatctaaagt aatagaccct ttcatacgcg
    20941 tagctgtatt tcttgctggg ataggcggaa tctctcgaaa acgtgcatct acgcttcgta
    21001 tgacatctct tgcatcgtct tgccgattcg tatgcttccc taatatatat tcgtaaaatt
    21061 cttcatctac aggttccgta tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt
    21121 gctcctgaac gttttctgac agacctgcat ctccgatgta aacaatatcc gtgttacttg
    21181 ctaatagatc tagagcgcct gcaagaggat ggatccctcg atgttcttcc aaagttggca
    21241 atccttgata tagtgggcct ctcaactctg tatctcctgg tacaaaacct tggacaagga
    21301 gaccttgaat tttgagccat tgattttttt tctgatacca ttctttgtcc agccctgttt
    21361 cgggacgtgg ataataatta tgccaagctt caatctgtga gaaatcagct tctgcttctt
    21421 tcaattcatc gatatcttta ggcgtgatcg tgctagcatt caaactgatt ttcaattgat
    21481 gtgaccagtc ggcaatctgc tgattggaaa tatgataatc cattcttaaa cctgttacac
    21541 caattgcttt caaaggcgtt agatcttcta atgaaaaacc cgctcgttcc aatgcctctc
    21601 ctgaaatatc gaccatcaat tctagtttat tctttttcgc ttcgcttcct agttcgatca
    21661 atcgcttttg atatgccgaa acatcatctt ctggtatatg catggaagtg aaaataccga
    21721 caaagccatt ggatgccatt tggcggatat actgtttctt ctcttctgac aaagaatcgt
    21781 tcataaatat tgaaaagcca aacatattat ccctcttttt ctgtatccgt cttcatttct
    21841 atgactagag tatatatcta aagaaacaaa atttcaatac ttttattaaa ataattaaat
    21901 ttcatttcta aaggttgagt ttatattatc aaaagaattg tcataaccta aaaaaggcac
    21961 tgctaagcaa aagcagtgcc ttgaaatatt ttatttaaat aacacaaaaa cataattact
    22021 cataagaaac caattctgta ttatctacca tgaatgtaaa gaacccctca ttaggcatcg
    22081 gatgaaaata agcattgatc actcttatca aggcattatg gcaaactaac agatagacat
    22141 tttcttcgtc ttcgatgcat tctttcaata aaggaacgat ccgtgcatac acatctaaga
    22201 ctgactcccc atttgggaat cgtacagcga acgctaaacg tgctttttga aagttctcat
    22261 ctttgcttgg cagtccatca taatccccaa aatccatttc taccaatcgt tcatcaacgg
    22321 tcagcggcaa gctgagacga tcagcaactg cttgtgctgt atctcttgct cgttgaagag
    22381 gggaatggat gattttcgtg attggaacag gcaaatctac taccttttct gctagtttct
    22441 gtgcctgcat ctgtcctttt tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt
    22501 gtgcgttcca agttgtttct ccgtgtctcg ttacataaag catgtgattc tctccttcgt
    22561 tggcaaagtt ccccttaacc ataccttgtt gttcttgaga ttacaagggt tagattctct
    22621 caagagaaat aatctcagta gatactttat ccaggaaggt ttgatcatgt tccactagta
    22681 acatggcagg tttcacgttc aagatcagtt gttctaattg ttcttgattg aagacatcca
    22741 aataattcaa tggttcatcc catgtatata gttcagctgg ctgtgataaa gatttagcca
    22801 attccacttt tttccgttgg cccatactca tctgctcgat cttgttatga aaaacatctc
    22861 tttccatccc aagctttcgg aggttgttca aaaatgcttg gtagtctact tggtttttct
    22921 ctgcaaattc cgccaacgtt cctcgattgt cttcataatt ttggcttgca taactaatgc
    22981 tcagatgttt tggctggtat ttttctccta taaccttgcc gttgaatgcc cccagaagat
    23041 aatggataat ggatgacttt cctgcaccgt taggacctga aatcgccacc cgctgatgag
    23101 gctcgattgt aaaatgaatt ggctcgaata acagattttc ataccctaat tgaagatctt
    23161 ctacgcttaa aagtcgctta tggtgagacg cttggctatt catcgtcaaa gaatcgatat
    23221 attcgatatc ttttaatagt ttttctttct cgctgatctg ggtctccatc cgattcacga
    23281 ttgctttgga tcgtttcatc gtccgtgcag catcagcacc cactgctcct ttattcactc
    23341 gtctagattc agtatcgatg aatccgactt gtttctttgt tttatctcct tctcgggaac
    23401 gagaccattc ggctttttca gctgctgttt tctttagcct actgacttct tttttcaatt
    23461 tttcattttg agccatttca aattcatcac gaagtttttt ctgttcttca tagatagaga
    23521 aattcccttg ataaagttcc agttgacttt tttcgattgc taaaacatgg tccactactt
    23581 catcgataaa tccccggtca tggctgacca cgatgaagcc ttgttttttc tttttcaaat
    23641 aagccgctac ttgttttcta ccagagatat ccaaatgatt cgttggttca tcgattaacg
    23701 ggaaatgagt gtcatccaca aataaaagtg ccagtaggac ttttgtcttc tctccccccg
    23761 atagtgtgct gaattctctc cataagattt caggatctgt ttgcatcaat tggagctctc
    23821 tttcgatttc ccatatctca aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt
    23881 ctttatcttt tgtctgttgc gggaaatagg caaattcttg ctgatggatt acttgccctt
    23941 gataaggtag tttgttttgt agaatattca gtaaagttgt ctttcctcga ccgtttcgtc
    24001 cgataagtcc tagtttccat tgtgtgtcaa aatttagatt tgcttgttca aataataatg
    24061 tgccttggct gtcgtagccg aatgtcagat tttttatttc gattttagac attttccatc
    24121 acctcgtaaa aaaagaccag tcctattttc agaactggct aaagaggatg caagggttgg
    24181 atacacacaa aaagacccat acatcgaaaa aaccaagcca atcccgaaaa tctgcacact
    24241 gcttccttgt ctccacaagg tacagtctga atgcagcttt catcaatcga cttaattttt
    24301 caatgcacgg atccctcact ttgtcctata tttgaaatta cgatagcata aagaaaatga
    24361 ttttgtcaac tacttcggga ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt
    24421 tgttttttct aatgtttgcc tgtgttccat tgcttcttct aaacctattg gacccaaatg
    24481 aatacttaag acaattggga gaaaatcaga aagtccttct agctcttggt ctctacttcc
    24541 gcaaatagcg attattggtt ttcgatattt ttttgcaagc actgctacac cgtatggaac
    24601 ttttccgtgg atcgtttgct gatcgatccg cccttctcct gtaatgatga catctgcttg
    24661 ttcgatcttt ttttccaatc cagtgatttg gctgactaaa tcgaaaccag aaaccatctt
    24721 cgctcccaac aaaaaaaggg ctccaccaat accgcctgct gctccacttc ctggaatatc
    24781 atctaaatcc acctcccaga aatcttttat ttgctgagca atctttcctg cttgtttatc
    24841 taagaaagct agcgtcgtat ctattcctcc tttttgtctt ccaaattgcc aagcagctcc
    24901 ttgttgacct gtatatggat tggtaacatc tgcagcgata atcaatttta ctttagaaac
    24961 aagaacgttt tttcgatcaa aatgaagttt tttagtagat aataaaggat ttccttctgt
    25021 gtacccttct aaagaagcac ccaaggattg gagtaatcct agaccaccat cgctacaccc
    25081 gcttcctcct aaagtgacaa taatttgttc tacttcttga agcagtgcat ctttaataag
    25141 ttctcctaag gcataactgg aagcaagtcg gatagtttga tccgttggtt tatcaagtag
    25201 atctaacccc aatgcttgtg cagattcgat gactgcggtg ttttttcctt gccaagttgt
    25261 caataaatag ctgatttttc gctgacggaa taagagatcc atcccatcaa tggtacgcca
    25321 tgttcccctg agcgcgtaat agattgcttc tgcagtattt tctccgccat ctgcaatcgg
    25381 cacattgatt tttcttcctt tacgatcttc ccagccattt agaactgcct gatttagttc
    25441 aaatgaagaa gcacttcctt taaatgaatc aattgccgcc aaaatatcca tctgtatccc
    25501 tcactttctt cttaagtaag acactttttt tcattttttt caaacaaaac aatgctattt
    25561 tcttccattt ttctatatac taaaagggaa ctcattgaat aaaggagaga aaaaacatga
    25621 aaatcgcaat agctggggcc ggagcaatgg gcagccgaat cggattgatg ctacatcaaa
    25681 gcggaaatga ggtcctcttg atcgatcgtt ggcctgctca cattgaagcc attcgtacaa
    25741 atgggttgat tgctgacttt aacggtaaag aagtggtcgc aaaactgcca atctattcac
    25801 cagaagaaat catcgaaagc aacgaacacg tggacttgat cgtagcatta acaaaagcca
    25861 atcaattaga tgatatgttt tgttcgatcc aatccattat tactgacaat acttatgttt
    25921 tgtgtttact aaatggatta ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa
    25981 atatcttatt tgggatcacg atgtggacag ctggtctagc tggtcccgga aaagtcacat
    26041 tgttaggtga tggagaaatc gaactagaga acttggaacc agaaggcgaa gcatttacaa
    26101 aaaaagttgt agaagtcttt caagaagcaa atttaaatcc gatctatagc cataacgttc
    26161 gttattccat ttggcgaaaa gcttgtgtaa atggaacatt gaatggttta tgcacgattt
    26221 tggattgtaa tattgctgaa ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa
    26281 tcgtatctga atttgcttct atcgcagcaa aagaaggaat catactagat caagaagaag
    26341 tgtaccaaca tattgcttct acttatgatc ctgacaacat cgggttgcat tacccatcta
    26401 tgtaccaaga cctgatcaaa aaccatcgtt tgactgaaat cgactacatc aatggtgcca
    26461 tttggcgaaa aggacaaaaa tatgatattg ctacaccata ttgtgccttt ttgacccaat
    26521 tagtccatgc aaaagaagga atccttggcg cagaataaaa ggctctctga tgagaatcag
    26581 ctgaatcagc agctggttct ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa
    26641 ctctcatgat gccataagaa ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt
    26701 aggtttttta tttgttttca acagaggaga ggtcatattc gtattgctca taaatagtgg
    26761 aatcgttagt gataaatcgt tttgcccaat tttctgcatc ataaaattcc aatagctctg
    26821 ataggattgg cttaaatggt gtatgcagaa attccggtaa ttcatctaaa tcacgttctc
    26881 gaagcaaaaa aatattttca ggacgataaa aatgataatg agcaatcgct tgattatccg
    26941 tcactagttt cttttgaaaa tacatggatt caaatgtccg caaagtcact ccttgctgac
    27001 cctcccgcaa cagttccaga atcccgcgcg attcccctgt ttttttcagg tactcttcat
    27061 aagtaagata tccaggttgt tcattccctc gatgtggaag aatgaaatag ttggtcgtaa
    27121 catctctttt tataaattcc tgcttatagc tttctgctcg ttcttttctc ccattattcg
    27181 ttgcaccaaa aaatagatcc gtctttggtt cgctagaaga aaaattcata tgtttcgaat
    27241 aaaatgaact attatgtttc agcccaagtt cttttgcttc caaaaaatca aaatgataaa
    27301 tctcatcgat tgccggttca tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga
    27361 agtgattcca aaaaaataac acaatttttg cattggtaaa tgggcgcacg cgtcgagcca
    27421 tctccatatc aaatccataa tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc
    27481 tagctgattt aatgaattcc gactgtagta aatcttttcc ggaccaaaca tctaaagagc
    27541 ggatggaagg caaaaaataa agtgatttat tttttgcaac tagcagtacg tcatttttgt
    27601 tcatcttttc cgcctcactt tctctgtcac tttttcctat gatagcctgt ttaaatagta
    27661 aaaagccagt aaaacgtttc ctatatttgt atgaaaattt aacatttgta tgaaaatatg
    27721 atgaaataaa tcattcttct attaaaattc gcctaaaagt tttctgaata gttgtaattg
    27781 tctgaaaatt tgttatcatt agtcattgtt aaatattgct atttattctt aatataaatt
    27841 ttgattgtga atttaaaaga aagaggtcat ttatggaaga taaaaaattg aaaaaacaac
    27901 ttagctccag acatatcacc atgttagctt taggtggagc aatcggtgca ggtctattta
    27961 aaggaagtgg cgaagcgatt gggatcgccg gcccttctgt attgattgct tttttgattg
    28021 gtgggctgat tttatatatc gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg
    28081 acacgcatca cggattatct ggtttgatac gcccttatct aggtgctcat tctgctgatt
    28141 ttactgattg ggtctactgg tctatgtgga ttatcaatat catcgctgaa gctgttgcag
    28201 ctgcttcctt tttgcagttg tggttcccgc acattccagc ctgggtattt gtttttatgt
    28261 tggctgtttt aactacgatc atcaatcttt attcggtacg gttattcgca gaaacagaat
    28321 attggctggc ttttgctaaa atcagtgtca ttatcttact gatcattttt gctacttatc
    28381 ttgtcggtca gcaaatgttg ggcacaggcg ttttccctac ccttcagcaa ttgactgacc
    28441 atggtgggtt tactcctcat ggtatgaagg gaatcatcag ttctttgctt gtagttatct
    28501 attcttacgg cggttctgaa ctgattgcta tcacggtaag cgaagcagat gatcctaaac
    28561 gtgcgattcc taaagcaatc aaaggtgtca tgggaagaat catttccttt tacatcattc
    28621 ctttgttttt gcttttgatt ctttttcctt ggaatacttt agcaggcacg aatgtcagtc
    28681 cttttgtgat ggtatttgaa aaaatgaata tcccatttgc agcggatatc gtcaattttg
    28741 tgattgtcct tgctttgttc tcatctatca attcgggtgt gtatgcatct tcaagaattt
    28801 tgttcttccg tttgaaggat cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc
    28861 atcaagtacc gcaacgtgct gttttatttt gtaccagtgt tctttatctt ggtgtagctc
    28921 tttcttattt tgtcggtgac aaattatttg gctatctggc aggttccctt tcgtatacag
    28981 tattattgat ctggatcatt atcagtgcgg catcctttgt actggcacta agaaaaggaa
    29041 cattatggga taaaggaatc agcttttttg cattagctgt cttaggattg attttccttg
    29101 gtatcctgtt cacgaactcc attggggtaa ctgtattgac tggcctgctt tacctgttca
    29161 tttatttcag ttatcaaaaa aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat
    29221 agaaaatttt attttattta tgtggaatag atggagctat tgaagaaagg agttgtgaca
    29281 aaagtcttgt tgcaactcct ttttccgaat aaacagtctc atgacctctt ggtaaacggt
    29341 gttcaaaaac cctctgtgca aaataattga ttgttctcca tgctcatgac atgatagaag
    29401 aaaggaatga aaaaaaggag tttgttttaa atgaaaaagt attggaagtt tttgttgatg
    29461 atcggtgttt caacggtagt tatgtttggt atgatgtact tgaatgtcta tgcgctcgat
    29521 catatcttct tcagtcgtac acgagtattc atggcgttga tgatgggtgc agtcatggca
    29581 gtcatcatgc tgttgttcat gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc
    29641 ttagccgcta gtcttcttgt ttttgccggt tccttgttta tggtacgaag ccaaactact
    29701 gtcaacgaca cagcttggat gaaggccatg attcctcatc attctatcgc tatcctggca
    29761 agtaaacgag ctgatttaaa agatcctgaa gtaaaaaaat tagcggaaga aatcattcag
    29821 gcacaagaag aagagatcga caaaatgaaa aaaatgatca aagaaatgga atgaccatta
    29881 tccgtccaaa taaaaaaaga ggatgttcca gaagttatca gcttcaagaa ataagaagaa
    29941 gctacttctg gaacgccgtg aataaggggt tgtgaaaaag ctgccctgca tcaagtaata
    30001 agaacagcca aacaaaaata gcttctccta tttttcgttt ggttaggctt attaccgcag
    30061 atgcaagctt ttgaacaccg tttattaaga ggttgtaaaa tcagcgttct gacctgagta
    30121 ttagagagac aaatggaaaa atagttcctc atattttgcc attttttgag ctaataccga
    30181 gggtcagctg attgaacacc gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt
    30241 attaagaaaa attttgtgaa atggctttcc acatttttgt ataaatttgg cttaataccg
    30301 agaaactggc ttttgaatac cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac
    30361 aatccctttt tatgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt
    30421 ttcacaaatt tttttgagat tccccaacct gtgaaggcac tgaccatcga aaacacagga
    30481 gaaagcaaac tgaaaaatac gaatggcaag taagtgagtg taggaatacc aaatgtactt
    30541 gctacgaaac ttccggcaat tccccaaggc actaagtagt tgattactgt tccaccatct
    30601 tctagcgttc tacctaatac agttggatca aatcctgctt tttcataagt ttctttaaat
    30661 gcattaccag gcagaataac agataggaat tgttcaccaa taaaaatatt tactccgata
    30721 cttgtcatca atgttgtaaa aatcaaattt ggtgtgttcg tcatctttct tgatactcgt
    30781 tccatcacgg ttgagatcaa gccaaattcg atcaacaatc cacctaatga caaagttaga
    30841 ataatcaatg acacagtagg catcatactt tcgattcctc cgcggctaag cagtgaatcg
    30901 atttgttggt ttccagtatt tgaaacaaac cctgattcga tgaccccagc caacttcgtt
    30961 aaggaagttt gtgggttttg aataaaaatc attcctactg ctacagcgat attcaacaaa
    31021 atcgttaaaa ttgccggcac ctttttccat gcacacagta acattaaaaa taaaggaatc
    31081 agtgaccaga ttgaaatagt aaagtttgct tctagaacat cagtcacttc acgaatagat
    31141 gaaagacttg cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca
    31201 aatgccggga cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca
    31261 atagctgctg ctagatttgt tgattcggat aatggagaca tcttatctcc aaaaacggca
    31321 cctgaaacaa tagctccaac aaccagcgct ggatttaagc ctagtgttgt cccaataccg
    31381 aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg
    31441 attgcacaca caacaaatac agaagggaca aaccactgga cactgatcaa tttgaaccca
    31501 atgaccatta gagttggaat gatccctgct tgtatccata cagcaatcaa tgaaccgact
    31561 aaaataaaaa taaagattgg gatgattcct ggtttgatgc cattgatgat tccttcgtgc
    31621 agccaatctg tcgggtatct ttttatcact gcatacaaca tgaccattcc gatcgctgct
    31681 aagatcgtga tattaggtga gacctttaat ccgacaacgc caatcgcaat gcctaaaaca
    31741 ataaccatta atacaagtgt tgcttctaaa aaacttggtg ttcctttttt tcccatgata
    31801 atctctcctt ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc
    31861 cgcaattcat ccgcgattcc tcctttataa aaataaaaag acctgtttta aactaaggac
    31921 gaatgactcc gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaactttt
    31981 attcaaatgt atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac
    32041 caccgacttt ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag
    32101 tttaccttcc ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa
    32161 attattttta taactttcac acacaagttt attttttact agaaagtagc gccctcttgt
    32221 ttttttcttg ctactatttc ttgatgttct tgcttgattc tttctaatag ctttggctct
    32281 ttcaacagat caagagctgt attggcgagt agttctgctg caatcgcaat agaagcaagg
    32341 cctttgtcac ttttagcagc agctttgaat tcttctgtgt gtccggcaat atatgtctca
    32401 gagattgcta ccgtaggctg gattgttggt atgacttggc tgacatttcc tacatcagaa
    32461 gaaccgagac ttgttctttg ttccgtttct atttcttcat ctggaacacc tgcttctttt
    32521 gcatgctgga agaataagtc atcaaaagaa ggagtaacga tgacatcatc aactgcattt
    32581 tggaacaatc caaactcaaa agtcgtgccc gtacttatcg cagcacctcg tacgatattt
    32641 tccacttttt catatacttt atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt
    32701 ccagcagcgt aatctggaat gacgttggca gctactcctc cgtctgtaat gatcccatgg
    32761 atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccattaaa cacttggatc
    32821 aatgcttcca aagcatttac tcctttttca ggtgctgctg ctgcatggga agcgacaccg
    32881 taaaatttga tgtcgactgg atcattcgct aaagattctg tcgttttccc ataacgaaaa
    32941 gctggatgga cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg
    33001 cctttagctg atccgttctc gcccccttct tcccctgggg tgccatatac acggatttct
    33061 ccaccagttt catcaatgaa ttgcttcact atgctggcgg ctaatacgga gtaagtacca
    33121 aacaaattat gcccgcatgc atgaccaata cctggtaaag catcgaattc tgccaaaaag
    33181 gcaattgtgg gaccgggttt attggaaata taacgtgcat caaaggctgt tcggtgtcca
    33241 gctacttctt tttctaccca aaacctttct ttttctaatt gtttaatcaa cgctgctgag
    33301 gaataaaact catagttact tacttcagga tgttcgtgga tatctagagc aagtgcttga
    33361 tattctggta aacgatattg gattagatcc ttcaattggt ttgttgagat tgttgttgtc
    33421 ataaaaaatt cctcctatgc tagtcatttt tcttattttc tcccgaatgt ttcccatgca
    33481 ggcacgcttg atccttttga tgttttctcg attactttga ctgtgtcttc tgtttgataa
    33541 gcgtctacta ttttttgata cgttttattg ttttcatctt cttttcttgc aacgatgata
    33601 ttcacataag gacgtgcatg gtcatccact ggttctaaga agatggcatc agaagcagga
    33661 ttaaatccag catctacagc tacaccgcta ttgatcactg aaatgtctac atctgacaaa
    33721 gcacgtgctg tttgggaagc atctaattca gaaatgtcta gattttttct attctcggta
    33781 atatcagaaa cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa tccagcagtt
    33841 tgcaataaca gaagagctct gccgccattt gtaacatcat tagggattgc tacttttgct
    33901 ccgtctttga tatcagctac atccttcaaa gtgttagaat aaattcccag cggtgcattg
    33961 acagtatttc cgatcgatac taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag
    34021 aattgatgtt ggaaagaatt caaatcaatc gatccatctg ctaaagccac atttggttga
    34081 ttgtaatctg taaatttcac gtattccaaa ttaatgttct cctttttcaa acggtctttc
    34141 acgttgtccc atacatctgt atcttctcca ataatcccta gttttacgat agtttctttt
    34201 ttgtcagttg tatctgcttt tccttggcta caccctgcca accaaataat tgctacaaat
    34261 gtaatgacta caaaacgaat aatttttttc attttttctc ctccactttc aatgtgtaat
    34321 ttttttaatc agttgattac tgataaattg acttaaaaaa actaagatta gaatcaataa
    34381 tgtagccaca atcgttacat ctgtttggaa tcgattatac cctctcgata tagcaagatt
    34441 ccctaatccg cctgcaccta ctgctccagc catggtagtc agtccgatca cgttgatgat
    34501 agtcaacgcc gagacccgaa tgataccagg caaaccttct aacaaataca cacgtaaaat
    34561 gataccgatt ggactggttc ccatcgcttc tgccgcttcg atcaccccag gatcaacttc
    34621 tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca actccaaaaa tcaatggcac
    34681 taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag
    34741 agaaagcaag ataatgaaag ggatggaacg aatgatattg atcagcttat caagtatttc
    34801 aaatagcaga cggttagcta gtaatccatt agggcgtgtc actactaacg tcacacctaa
    34861 gatgattcca atcatcccgc caatgaatgc tgtccaaaaa gacatatata gtgtttgctt
    34921 cgtcgcttcg acaaattcat ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa
    34981 atcagtcatt ttatcttcct ccttcgatca ctttgatacg actatctaaa tcgttttttt
    35041 gtaaaatatt tatttttaca ccagatttga tcaaataatc caatgctttt ttccgctgtt
    35101 tggtttctcc agctaaagta acgatcaaac tccctaacgg cgtttcttgt aataactcta
    35161 cattgccata taaaatattc gctgttactt gatatttgct gtacagatga gcgattagag
    35221 gttcgttcgt ctgatttcca atataactta attccaccag ccatttattg gcgatctgat
    35281 cggcaaaagc agaatgggca atgatcgttt ctaatgcttg gtcaagatga gtagctgttc
    35341 gaataaaatc tttggtcaat tcttcttcag gattgctgaa gatctggatg ctgctacctt
    35401 tttcaacgat tcttccatct tccataaccg ctaccttgtt gcagatttct ttgacaactt
    35461 gcatctcatg agtgatcagg acgattgtta aacctaactg gcgattcaat ttttttaaca
    35521 atgcaaggat ctgaagggtc gttttcggat caagtgcact ggttgcttca tcacataata
    35581 ataccttagg ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt tgtccccccg
    35641 acagttgttt aggataagca gatgcttttt cttccaaacc gacaagttcc agtagttcat
    35701 tcactttttg acgacgttcc tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt
    35761 tgtcaaaaat agttcggctt tccattagat tgaaatgctg aaagatcata ccgatggcct
    35821 ttctttcttt tcgcaattct ttgggagaga gctgactcag attcgtctga ttgacaatga
    35881 cttctcctgc tgttggtttc tgcagcaaat tcatcacacg aactaacgta cttttccctg
    35941 cacctgagta accgacgatt ccataaacat cgcctttttc aatcgttaaa tctacagaat
    36001 ctactgcctg tatctctttt tctttttgtt tgaaacatac agaaacatct cgcaattcaa
    36061 tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc
    36121 ttgaggacgc ttttttgcgt gttaccacct catgttcaag ctgtgttcac acacaactcc
    36181 tcttcaagta cgatcatact ctagcaagat atcggtcgca gccgtcatcg ccttacatct
    36241 atgctcggga tgaattgctc gatggccatt ttccaatagt cgttccttgc ttcttctcag
    36301 ctaccgaagc tctctgtcaa aggttcttct atcgtactta ccatttcaat gcaaataata
    36361 ttcaaaaaaa aagtcctttt actaacataa actatgtcag taaaaggacg acgtatcgcg
    36421 gtaccacctt ttttcggaag tttccttccc tcaagcccaa catctcaaaa agagagattg
    36481 gcgttcgttt taacggtcga acagttccga atttgcctac ttatcgacaa attattctcc
    36541 aaggccattt tcagcttttc ttacttgact cttctcacca gccagagcct ctctgcaaag
    36601 tttccaaact tactttcctt ttcaacgaaa tcgatatttt gttgttggga ttaatctaca
    36661 tgaggaaaac tgaaaagtca agtacgaaat tcaaattatt ttttctatat ttatttttct
    36721 gtttttttat ttaacatttc aataacagtt cgaagatctt ttccttccgc aagaagtttc
    36781 aatttttcct tttcttcgac ggtataattt cggacaaaac tggaatcagg tatcaaaaca
    36841 acgcctaatc ctgctcgtga agcagctgtt gctcctacta aagaatcttc cactgcaaag
    36901 gctgaactag cttctaatcc tgttcgttcc aaagctgtca gataaggaat cggtgaaggc
    36961 ttgttctccg acacttcgtc tccgaatgta aacgtgtcca agtaatcagc taatttaaag
    37021 tgatccaaga tatctgttgc tcttttcgta acagtggtcg tagcaatgcc tagtttcata
    37081 tgacgttctt ttaagtctgc caatatttct tttgcatatg gttttaagtg gatggctccg
    37141 cttgtcagct gttgataaaa atattcttca cgcttcgctc ggacttcctg tactttttca
    37201 gggctacctg ctgctttcac taaaatatca aaactttgtt tccagttttt tccgccccaa
    37261 gcgtcaacga tttcttttgg gatatggata tcaaattgct gaaatcccca aagccagcca
    37321 tcacgatacg ttttttctgt gtcaactaac aaaccatcta aatcaaatat tgcacctttc
    37381 atctatacat taatcctttc caaaacaata cacatagttt agcacgactg gtccaaaaag
    37441 aacagaagca aagaaccagc cttctcgttt ataagagttc ttggaatcga cggatataga
    37501 ttttcttcac agctgtcaca agcgccagat aagcgatgac tgtagcaaat aaccataacc
    37561 agtaattgcc aggaagtggc atcaagccta attcagcacc aaaacttgta aaaggaagga
    37621 cagaaccgac aataattcct aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt
    37681 ggataaatgg tactttcggt gtacgcagtg catggagcac taaggtttgt gtccatagtg
    37741 attcgacaaa ccatcctgca tggaataatg cgatgaaaat cactcgctgt gaactatcca
    37801 atgtatggaa acttccgccc actacagcag gacaaataac aaaatacatc aacaagtaag
    37861 tagtaatatc aaatatcgaa cttgttggac caaagtaaac catgaattcc ccaatactag
    37921 atgcattcca tttttttggc tcgtccaaat attctttatc catccgatcc catggcaagg
    37981 aaatgcagga aacatcatag atcagattca aaaacaaaag ttgtaaaggc agcattggta
    38041 aaaatggcag gaacgtactt gcaatcagca cggaaaacat attgccaaaa ttagaacttg
    38101 ctgtagcctt gatatatttc atgatattcc caaaagtttc tcgtccagaa agaaggcctc
    38161 tttccaaaat cgtcaaatct ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat
    38221 ccacagctgt atctacagaa ataccgatat cagccacttt cattgcagga gcgtcattga
    38281 taccatctcc taaaaatccg acagtatgac catttttacg taaagctgtc gtcaaacgtg
    38341 ctttttgctg aggattcaat tttacgaata cttcatggtc ttcagctact tgtgctaatt
    38401 catgatcact catttccgag attttctcac cggtaaccaa ttcattgatc tctagaccta
    38461 cttgacggca cactgaacgt gtaactaatt cgttgtcccc agtcagaact tttacagcta
    38521 caccatgttc tttcagagct ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga
    38581 aagcaagata accaatcaaa accatgtctg actcatcttt gactgaaaat tctccaacga
    38641 cgcttggatt ggtcttttgt gcgacaccaa tcactcgtaa gccatcttca ttaagtgact
    38701 gtacttttgc aagtacggct tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa
    38761 gatcaataaa tttagaaatg ttcagcattt cttcaatcgc gccttttgtg atcatttgtg
    38821 ttttaccagc tggatcagca accacaacac tcatacgacg acgttcaaaa tcaaaaggaa
    38881 tttcatccac tttttgataa ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa
    38941 tgatagccac atctaacaaa tttttcagcc ctgtttgata gtagctattt agatacgcat
    39001 ggcgcaaaac acgatcatct tcatttcctt cacaatccat atgatattca agaatgattt
    39061 tatcttgtgt caatgtcccc gttttatccg tacataacac atcgattgca ccaaagttct
    39121 ggatggcatt caaatttttg atcactgttc cttttttggc catcacactt gctcctttga
    39181 ccaaattcgt cgtaacgatc attggaagca tttcaggtgt tagaccaact gctacagata
    39241 gtccaaaaag aaaagcttct agccaatccc cttttgtaag cccattgatg acgataactg
    39301 ctggagccat caatgacata aatttgatca gtagcatgga tgttttatgg atgcctaatt
    39361 caaagcttga ttgaattggt ttttcagata gcgtttctgc tacatgacca aatagtgttt
    39421 catttccaac agaaacaatg attccttccg cacttccact gatgacatta cttcccataa
    39481 agacaagatt ttcgtaactt gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct
    39541 tttctactgg atagctttcc cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg
    39601 acaataaacg catatccgct ggtatcatat cccctgctga aagccgaaca agatctccac
    39661 agacgatttc ttccatagga atctctgtgt actttccatt acgttttaca gctgccgtta
    39721 ctttcacaag tgatttcagt ttttcagccg cttgattgga acgtacggac tgaatcaacg
    39781 tcattgtccc gctgacgata accatagtga agataatggc tacaccgaaa agatctttat
    39841 ctccaggagc tgctaataca tattccgtga tgaatgaaat aattcctaat gcgatcaaca
    39901 ctaatgtaaa tggcgtgatg tatgctttca atatttctac aatgaatggt gtttttttgc
    39961 catatgaaat cgcattttca ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca
    40021 atccatttgc tgatgcatgg aatagttcaa ataattcttg tttcgttttt ccagcaaaag
    40081 ctgcatagat tttttgtgta tctttctttt tttccatctc aatctctcct ttgtgtccaa
    40141 aagacagctg ttctcgctgc ttttggtctt tagctattca tcggatcgat agagaaaaaa
    40201 ctgaaagggg ctgagaacag atagataata gccctggcct ttcctctaga tccttgtttt
    40261 tcgaatgact gtcatccatt tgttctcatc tcctttacta agtaatttta attcatgact
    40321 tccaatcatt tttcgaatag ttatttttcc aattagacca cctccttttt atttttcacc
    40381 gatggggatt tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa
    40441 agcatattaa aaaaagcttc tcagatctca tcgttgagtt ttgacactgt acaacgtaaa
    40501 aagcagatac ctgcttttag ctaccttatg aaaagcctta tctcgacaat tcctgttcta
    40561 cccattggcg tctctcgacg tttttgggca gtagcctatg tttgtatagg agcctcacct
    40621 aacaaatctt attcggtttt ccttcgttaa tataacaact aaaaaagagg atgtcaaatc
    40681 aattttccat gtttttcata ctttttttaa aatacacacg ttatattcct gtctcacgcg
    40741 gttttatttg tctcattttc cacctcccgc tataatagaa atatagtagg aaacgaggtg
    40801 ctgttatgga aagaacactt gttattatca agccagacgg cgtgagacgc catttagtcg
    40861 gaagcatcat ccaacgtttt gaagcaaagg gactggccat tgctgaaatg aagtttgaaa
    40921 ctatgactcc tgaattagca aaagaacatt atcagcactt gactgagcgc agtttttttg
    40981 atgaactgat cgattacatg acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg
    41041 aagtgatcga tatcgtccgt aagatggttg gagcaacaaa agctgccgat gctgttccag
    41101 gtacgatccg aggagactac gcgctgccgg ggactgaaaa catcattcac gcttctgatt
    41161 cccgtgatgc tgcggtaaaa gagatcgccc gctttttccc gccattcgat ataaacaaac
    41221 cgtctgaaaa agcggcgaag tgatacaaat tctaaactgc tggtctagtt gctgggttca
    41281 gcaatggcca gcagtttttt ctctaaggac ctccgttgat tctttcctaa catatattcg
    41341 agcactgtga agaattcttg gtagtctttc gtcttttctt cccctaaagc agcagaaatc
    41401 aatctttgat aataatctgc catcaatgtt tcatagatca ctgtacttct ttctttatgt
    41461 gctaagctcc gtttcatatc ttcattcaca ctacgtgcct ttttcaaccg accattttct
    41521 atataaaacg aagtcaggtt cattaaaaaa gtaaagagat actgctcatg attgttcaac
    41581 tgtgctaatc gtcgcattct ttttttgatt ttcaaataat tgttctcaat gaaccgatca
    41641 ctgaatatcc aaggaaaatc agctagaata gatagttcaa attgaccata gtctggtatc
    41701 tgcatcaagt attcttcaat gattcggcat tcataacgta aaaattcaaa ttctgtctca
    41761 gatacttctg ttttttcgac catgtgaaga ttcaaaacca aataatacag atcttctgtt
    41821 ttcttaaaat gcctacggtt attgctgatc tctttataag gatcaaagct gtcttcttgt
    41881 acccgatcta gcattcgtct ttttttcttt aactttattg gttcatccag ctggtttcgc
    41941 atccattcat attctgcaag tgtcatgttc attcgctcta aaaatgatct caatgttaca
    42001 aatgacattt ttttcactga atttgtctca atcccgacta gagaagtctt aaaacagatt
    42061 ccttccgcta attgggactg tgtcaacttc ttgttgactc gaagtttctt gatcaattca
    42121 ccatcataca taatagttct cctttttaat ttaaattttt ccacattcaa taaatagata
    42181 cgttaaaaag gaaaaataca cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca
    42241 accttcaagc attagaatgt tgtctaacta ctgaaggttg gtttgattgt ttattgatta
    42301 tttttttgct gttcaacgtc tgttcttgtt actggatata agttggttgt tgttttatta
    42361 ccattttttg aaaagacact tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta
    42421 cgttccgctt catttttata gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc
    42481 acataaaacc gcaagagatt ttcctgattc agcttatctg aaagacttaa cgcagtcttg
    42541 actttttcct gatctttgga gatttcctca tcgattcctg ctgctttcgt atcgatgatt
    42601 tctcctgtct gattattgta agcttttcct cctaggacgg tgtatttggg tgtcacaaag
    42661 ttcccatttc gaaaagcaac tacttgatca tgctgagaag ataataagtc tgtaccaaaa
    42721 tgaagataat ctttatcatc gattcccaat agatgtagta atgtcggcaa aacatcgatt
    42781 tctccaccgt attcatgatt gactgtccct ttagtataac caggcatatg gatcatcaat
    42841 ggcactcgtt gcatctgtgc gttgtcaaat tcatcccatg tatctggatc tttaccaaga
    42901 gcgcttgcta aatctttatt atctgtattc gagatcccaa aatgatcccc gtaaatcaca
    42961 aagattgaat tttggtaaat accagatttt ttcaaatacg taaaaaattg ttccaatgat
    43021 tgatccagat aatgtgccgt tcgaacataa ttatctactg tgctattgcc agtattcaaa
    43081 gatggaaagt cgaaattctt gtcatccgta taataaggcg tgtggttggt tacggaaaga
    43141 aatttcgtgt aaaacggctg ttgtagatgc tcaagatatt gggcagattc tctgaataaa
    43201 tctttatcta atatcccata acctaacgtt tcgtcggatt catcaaaata agatcgatca
    43261 aagaaattgt cataacctaa gtttttatat acatgatctc tattccaaaa actagctaca
    43321 ttcccatgga aaacagcgct tgtatagcct tgcttttgtc caaggatcgc tggtgcggct
    43381 tgaaagacat tgtctgaccc aagttctgtg aataatgagc cttgaggtaa accaaatgtg
    43441 ccagtttcca acatattttc agcatcactt gtctttccct gtcctacttc atgaaagaaa
    43501 ttgtcaaagc tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg tgtgacttct
    43561 tgtccgtcta ccttcatatt aatcaaaaac tgctggaaac tttctaaatg gagaacgatc
    43621 acatttttcc ctttagcgat cccaaatgtt tcgggatttg gctctgcata atgtttcttt
    43681 gtataatcta aaacttcatc aatcccatta ctgcttgcat gcgcgcgaac actgctagtc
    43741 attcctgttt tgattccgtc ataaatagta tacgcatcga ttcctagata tttgacaata
    43801 tatgaacggt caaatgttcg ctgcagcaat tgtggacggt tggcttcgct cagcgctaga
    43861 tttcctgaaa aaataagccc tgaaagacag gtaacagcga tagccatcgg tttgccgaca
    43921 gggttgcttt tgataggtat tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca
    43981 gcgatatcca gccagtagat gacatcatgg ggcttcatca aagaaaagga gcttcctgac
    44041 aatccttgcg acactttcga aaagcctaat acagatttga ctgtaatgaa atctgtgaat
    44101 tcccgataaa aaataatatt caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa
    44161 agaatagcta acgctggctt tggcttttta atatacaaag aaaggccaaa aaacaataag
    44221 gtagtcgcaa ttgggttgat ccacaagata aagtactgga tcgtaccttc cacacctaat
    44281 gaaaaatctg catagtaggc aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa
    44341 acgattgcta aacgattttg gcacaatttt ttaagttgtg tcacgtatat cacttctctc
    44401 tattcacatt ttttcttatt ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat
    44461 cttaagattt ttttattctt tctaaagatt attccgtcca cttttcacag cataaaaccg
    44521 ctgcgatgta agcgattaaa ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc
    44581 cacggtattc aaaagccagt ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt
    44641 cttgtcgcag ctccttattt cttgatgcag gacagctttt tcacaacttc ttccttaaac
    44701 ggtgttctcc tgccaactcc tcggccacaa gtcacaatat ttataaaaca tgagaagctg
    44761 ttttttgaaa ttgttccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    44821 acggtgttca atcagctgac cctcggtatt agattatgtc aggacgctaa ttttacaacc
    44881 tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    44941 tataaggagc tattttttga aattgctcct tgtggctcag agttaaacgg caggctcaag
    45001 acctcttgat acacggtgtt caatcagctg accttcggta ttagctcaaa aaacggcaaa
    45061 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
    45121 aacctctgtt tttcattgtc cataggattg atattgtcct tgtgatgact cattcgtaat
    45181 aattgaggct tgattttctt cacttcccgt tatcgtttgt ccactgccag aatctgaagg
    45241 acttgttcct gtcgactcgt ttttttgaga agagacagtt gttttcttga cttgttcatt
    45301 tgtctttttc cattccgcta aacgatcggc gaccactttt ttgtctactt tttcagaata
    45361 agcggtgatc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg
    45421 cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat
    45481 aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt
    45541 ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga aattatctac
    45601 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    45661 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    45721 aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac tattctatta
    45781 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    45841 ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct
    45901 taagcctcaa atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt
    45961 gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca
    46021 ttggtccacg accagttatc catacatgtc cacatatgat agcctaaaca attggcacct
    46081 tcttgaatgg cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca
    46141 tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt
    46201 tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag
    46261 attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca
    46321 taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt
    46381 gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg
    46441 ataaccgtca aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca
    46501 atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac
    46561 gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt
    46621 ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg
    46681 atggctaacg ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct
    46741 gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca
    46801 ttatgagtaa accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat
    46861 ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc
    46921 ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca
    46981 aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc
    47041 aaacggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt
    47101 acatcctctt gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag
    47161 aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct
    47221 gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga
    47281 aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc
    47341 atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg
    47401 ttttaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat
    47461 tttcggaagg atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc
    47521 cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta
    47581 attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc
    47641 gctttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac
    47701 cgcttctttg gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat
    47761 aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc
    47821 tcgatcattc actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat
    47881 aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa
    47941 gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt caattttttc
    48001 tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc
    48061 gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat
    48121 agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc
    48181 tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta
    48241 acgggaaact gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat
    48301 acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac
    48361 cgcattcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga
    48421 gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat
    48481 atagcatttt tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt
    48541 ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt
    48601 ttctcttcca tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa
    48661 tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac
    48721 attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc
    48781 ttctgtctta aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg
    48841 taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac
    48901 acgcacgtat cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct
    48961 gacagatatc aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa
    49021 aaaagaaaca acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg
    49081 caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc
    49141 tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg
    49201 attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct
    49261 tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt
    49321 atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact
    49381 atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa
    49441 aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat
    49501 atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt
    49561 ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta
    49621 tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga
    49681 cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact
    49741 tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt
    49801 tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt
    49861 ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt
    49921 ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc
    49981 tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct
    50041 tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa
    50101 aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa
    50161 aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct
    50221 tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc
    50281 actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat
    50341 gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt
    50401 gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac
    50461 atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca
    50521 aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc
    50581 tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag
    50641 aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt
    50701 tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca
    50761 ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt
    50821 tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt
    50881 ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt
    50941 cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta
    51001 gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat
    51061 cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa
    51121 gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca
    51181 ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt
    51241 attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc
    51301 aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac
    51361 acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga
    51421 cgccactata tatagtattt agaaaaaaat catttttcag ccattttata gataaaacaa
    51481 aaataaataa cgtgttttca tcgatatgaa atgacttact tatagaagat actgggattt
    51541 cgtcctatat gccaataacg tgtattattt taaacaacaa atacttgttt cttttattaa
    51601 cgaaaaaatc attttgttct cgtgttatgt ttcactgata aatgatgttt ctctaaataa
    51661 aaaagccgac gaactaaacg atcaaaattt gatcattttt gttcatcagc ttctcctaaa
    51721 aatctttttg ttaacgataa atagctggat ttgtaaaaat atcacttgaa tcgtcactgc
    51781 tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc ctggaactag tgtttgatat
    51841 tgggctcgaa cgtgtactga tctctagcat gaagaataat ttcttttttt actgtataat
    51901 aactttcttt cctttttgga atagtacagg aaagtttttt tgtttccttt ccttcgacat
    51961 aaggtatacc agaccctttc gacaacgaaa ggttctcatt tttccttcat cgtcatttca
    52021 aggagggtgc cgatgctctg gacagtttcg atcgctccta aaccaaaatc tgtatattcg
    52081 attctttcta actctgattc tttcatacca ttcctgtctc aaggtacatc tattgtactt
    52141 ttttcttgta atccattctg cctctcctaa ctaattgtca aatcatccca agatatgtct
    52201 tcttcctctt tttcttcttg gatgatttct gcattcggat ctactggttt tttcaataat
    52261 aaaatggtta ttcctgtgac aaaagaacca gctaacaatg caccaatgaa tgcccatggt
    52321 ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa
    52381 aacatgctta atccgccacc tactgtccca ccgactcccg ttgctatgac attgcgtgcc
    52441 aagtcgttca atacgatagg gaaacatccc tctgtgatct gacagatccc cataggaaaa
    52501 gcggtcttca atgtatcgat ttcttgacga ttgagtatat ttttacgaat gattttccct
    52561 aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg
    52621 attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta
    52681 atccagaaac agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc
    52741 tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt
    52801 ttatttatgt ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg
    52861 aatattcaat catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct
    52921 gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa aaatactgcc gagaaatcat
    52981 cagaaaatga gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc
    53041 caaattcttc tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat
    53101 aaggctgatt gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg
    53161 aaaacaaagg atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga
    53221 cttcatatcg ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt
    53281 aaggacgatt gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg
    53341 atgccagcag ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta
    53401 attcactttc tgtcggcaac atcgaaccga ctggatattt tcctgagaaa atatcttctt
    53461 taattttatc tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg
    53521 ctttactatt actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg
    53581 agaagtgtgt tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat
    53641 tacttcttta taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc
    53701 atcgtctaga tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact
    53761 gacaagatcg atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc
    53821 attagccatc gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt
    53881 gccatcctct tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt
    53941 ctggtaacga ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca
    54001 gccccattca cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga
    54061 agttgctgct tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat
    54121 gtagtccact ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc
    54181 cggatcgact ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac
    54241 ttggacatcc gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt
    54301 gactggattg gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat
    54361 atccgaacgc aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg
    54421 ccatgcttgg aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact
    54481 taaagctgga aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt
    54541 actgctgtat ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg
    54601 atttttccag ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt
    54661 gatcacaggt tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa
    54721 tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga
    54781 aaaacgataa catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc
    54841 gtaatgatcg attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc
    54901 aaattcttca ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac
    54961 ggatagtcct tttccgtctc gatcataagc cccttctaat tgatttggca gcagttgctc
    55021 caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc ttattttaca
    55081 attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag tatatcggca
    55141 aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc tgctctaatt
    55201 gcttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc ttgtttgact
    55261 aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg aatcaaaatc
    55321 tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat agtcgtgatc
    55381 tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat accttttccc
    55441 atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac tttacccgtt
    55501 aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt cgcatgtttg
    55561 ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt acctacgaat
    55621 gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga ctctacacca
    55681 tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct ggagaaagct
    55741 tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca agcggcaatg
    55801 aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat tccaaataac
    55861 gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct
    55921 acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg catcaataca
    55981 tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatccccg atgcatgccg
    56041 aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat tggactaaat
    56101 ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc gattggtcct
    56161 aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac taaaatcatt
    56221 tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg aacccatacg
    56281 gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat cactgggatc
    56341 ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg atacacaagt
    56401 gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc tgctgtaaaa
    56461 gcaagcatga caggtaagaa ggtaaagaca ccatctgctt cagcaaacaa tatttgataa
    56521 gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa tcctttcaat
    56581 accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat aatatcgatc
    56641 agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc aagaccttcc
    56701 tcatccacga tcgattgata aacatctgct acattactgc caatcactac ttgatactga
    56761 ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg cgtatctgct
    56821 aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa acttttgaca
    56881 ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt atccattgtt
    56941 ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa
    57001 acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa caatccttat
    57061 gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa ataaatctgc
    57121 tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat ccgttcgaca
    57181 caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc atcttgctcg
    57241 tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt aatgatacgc
    57301 tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat gatactcagt
    57361 atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg
    57421 ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc cacacccgtc
    57481 ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg ataaaatttt
    57541 tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg tgtgatcgca
    57601 taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac ttttagttta
    57661 tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg aatatcagcc
    57721 atcagctgct ggaattggtt tttagcaaaa gaatctctta aaacaaattc tttttggacc
    57781 gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt tcccatacaa
    57841 acgacttctt ccccgaatcc atttttcgaa atgacgacat tgttattcaa aattttatca
    57901 attttcattt tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc gcctactgtt
    57961 tcttagctga attctggttt tgcctgccgt acagtaacaa tccatttctt attcaaaatc
    58021 atcataccat cgaattttta tttatgcaag ccttttcata taaaaaataa aaaaactgga
    58081 acacaagtct atctagaaaa caaccaaatc agagatgcac ctctttgcgt ttctcttttt
    58141 ttcagcaaat tggtttttcc ttatcgattc atatcctttg gctgttttta atctgtaacg
    58201 acttatgtcc cagtatccta aaatgttttt gtctctagat tgaaagcaga tctcatatca
    58261 tctgtcgcat cttttatcga tgtaacactt tccagttctc cttgtacaac tagtcgcgtg
    58321 acggcgtcca tgtcgccaca agtaattagt gtgacaattt cttttccctc gacttcattc
    58381 agtaattggg tatctgtagg agcaactttt tctttaaaaa atgttttgta tgtatacaca
    58441 ttttctaaat ctgtcaaata gatcttggca cccatctcta aattttctag cggtgtaaac
    58501 agcaattcag ggttcgttgc acgatggctt gctaaggcat aatttccttt tcccatttct
    58561 tgatctggag ataatgttcc tgctccgtaa agtagtgacg tattatccaa gcctttgaaa
    58621 accggtaaat tgattgaaac tgaagggact gcgatactag caatcactgg tagatctgtt
    58681 cctctcattt gcgacctcat gaccccttct gtcgtcattg gttctaccgc atcaaagtca
    58741 aaactgacat ctttatcgtt attctttttc agatcttctt ttgttacatt cgctatagca
    58801 tatttatctc ctgtctctct gacaaaataa tccttgatct gttcattgaa aatcaaagct
    58861 agtcctacta ataacaagag gaacaagagg atattgacta gccatctttt tgcttttcct
    58921 ttttttttca ttttctctcc ctccagactc attttactgt ttttcttttg ctctttccaa
    58981 cagccgaatg acacatttct acaactctgt gtattttttt cgtacacctg cgcttttttc
    59041 tacaggatat ttcaaattat ttttgatcaa tttttcttca atgatctcat ctagatccag
    59101 gtgaaggttg tctgccatca tgtatgaata gatcaataca tctgccagtt cttctttgat
    59161 tcgttctaat tgtgtttctt ttacttcttc cggcgttttc cattgaaaaa gttctaacaa
    59221 ttcagatgct tccaaagaaa tagagatagc caaatctttt tcgttatgaa attgtcgcca
    59281 attacgttca tctctaaact ggttgacttt ttccatacta ttcatttgct tgccttcttc
    59341 caatcacttc ttacttgatt aatcatacca tttttttaca taaaaaatag atgatgcttt
    59401 taaaaaaact cctgttttct tttatttaaa ataaatgtca tagaaaatca ataatttagg
    59461 tagccaccat gtgtatgcag tagcgtgtct tcttcccttg aggctttttg tttttcgata
    59521 cgttcttctt cccaaacaga caacgctact agaatctctc ttaatgattt tccttcttcg
    59581 gtgagcgagt actctacttt aggcgggact tggttatagg cttttcgatg gatactttca
    59641 tcttgttcta actctcttaa ctgctgggtc agcatttttt ttgtgatcgt agggatttgc
    59701 ttgcttctcc acgcctaaaa taagcgatat tgctcttccc tttatctggt gtaagcaaag
    59761 cagttagcat tttgacgatc gtgggttccg cttttcactt cttatctact tagtatcatg
    59821 aagtatactt tcgtactaat actaagtagc ggaagtgtca actactttgt ttaactacat
    59881 agcaaaagaa acagaagggt gacaacggtc atcacgcttc tgtttctttt gaatatgctg
    59941 tgttgttgtc tctatctccg tgtagacttt cctcaaactt atcctttgac agtaagtcag
    60001 taggctgatt tgctctttct agatgccaga tacatttcta caaaaaaata gtgattctgt
    60061 tttgaaaatt cgatttttag ttctgcattt tcgtaaacat ccaacatttt tttcgcactg
    60121 tatagaccta acccgccatt ttttttagag gtttttcctc ttttgaccat ctccgatagc
    60181 gtcaaattcg ttggatttgc cttgttcgct atactaaaac gtgatccctc tttttcttcc
    60241 agcagattga tcgtaatcgg actctgatcg gctacacgat gatcgaatgc gttagaaata
    60301 aaaatagata aacagcgaat aaaaacaacc agatcaatcg ggattgtttt cacttcgaca
    60361 ggaataacta cttggatcgg gattttctcc gattctgctt tttctagaaa ttttaaaaag
    60421 acacccctga cagctgggga tacaatattt gttagctgtt gtatttgcgg tttttcaaaa
    60481 atagctgcag aatgatcttc taatatttct aaaaattcca acgtttcttt tttattttcc
    60541 tgaagagtaa tttttaatga aaacagtaga cttttataat catgtttgaa ttcctctaga
    60601 tgatggtaat attcttcatt tatttgatac agttgaagcg attcctgatg ttgtctttct
    60661 tttacttgga aactgatcaa cataatggaa aaaaacaaag aaaaaaaact gatgactatc
    60721 gacaaaagag atagaagcag ataattagat gagcggaagt gaacacttcc caataccaag
    60781 taaatcacgc cccagcttaa taaaatacaa atacctccaa aatagtgttc agttggataa
    60841 gtaaaaagca agttgagaaa tctcgtctgt ttgtctatac tttttactaa tagatagaaa
    60901 ataaatgcga taagtagtgc aaaaaaactt gctgttccaa gatgtctctt tcctaaataa
    60961 tccatcagat caaaaaccag aaaaaagctg agtccagtga tagcaaaaaa aagattactg
    61021 atcaaacagc tgataaacag gtttttcgta gtaaaataaa aaagaaacat caataataag
    61081 ccaactaggt aaggaaagct cactttaggg ataggaagta aaagtgtcac caccatcaaa
    61141 gctatacaac caaagctgtt ttttactagc agttgtttcg aaaagaataa atagatacct
    61201 aaatctaaaa tcaaagtaaa ggtcaaataa agtgtcactg ttgcgaccat cttattcctc
    61261 cattgttttg tttcaaaatg tacacatatt tttttaatag gagcctgcat atgaaaaagt
    61321 attttatcat agaagataac cagtttcaca gcgaatggat catcagagaa ttggtcaatc
    61381 atcatatcga gaatatcgtg cagctcactt ctatctctca aatcaatcag ttgatccagt
    61441 tgggcactat tgataatcac gatgttttct ttatcgatat cgacttgaag caatcactca
    61501 caggtattga tgtcgccgaa caaatacgta aaaaaaatgc acatagtaat attgtttttt
    61561 tcaccgctta tacgaatttt gcagtagaga tcatcaatcg gaaaatcctt ccgattggat
    61621 atatcagaaa agacaggaat ttatctgaac aaattcgtca agtgctagaa cagatcaatt
    61681 ggaaagaaca gcagatcgta aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc
    61741 tcgtcctgta tcctcgggaa attttatata ttgaaagtat caaaggtttg aaaaaccaag
    61801 ttatggtcaa aacaactaca gaagataaat tagtgaatac ttctttaaaa caattgaaga
    61861 cagagttaga aaagtatgct tactttttac ttttaaaatc ttactgtatc aacttatcgc
    61921 aattgcagaa aattgattct gctcattatg tgttagaatt tttcaacggt gactccttac
    61981 tcgttggtcg aaagatcttt gaaaagacaa aggaacgttt ccatgacttc caaaaaactg
    62041 cttcgagttg atgaataaga gctgagacgt aagcatagca gatttcaggg agtgggcctt
    62101 gaatcggctc cactccctga aatcgttgat atgattttac gcttcctctt ctgtaggagc
    62161 atctgttaac gaccatgtga atgttgaaac gtagttgtct acagcataat tggatacagg
    62221 aacaaataat tccactgatt gtctcccttg ttccacattc tctcctaatg aataaatcca
    62281 tgtgccaatc ccttcatttg ctcctgcagt tactagatct actgcctgat ctgctgggat
    62341 ttcgattcct tcagcgccag ttccagaaac ggtagatggc attccactgc tattagttgc
    62401 gctatttcga atcacggcgt ttttgaattt cacctttgaa ccagttagtt tattcgtatc
    62461 agatgttgta ccaataaatt ctgttcgttt gactgataat ttccaaccgg cataatttcc
    62521 gcttttatcc gttacttgta cgaaattctg tacttgtgtt tgtgctccag tactgtccgt
    62581 tccgctttgt aatgcagcat atactgtttt tgctgtacct aacttgattg ttccaaaatc
    62641 aagatctgga gcttggtcaa tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg
    62701 tgtaaaagga gtatcaggat catttggatc aacaggagga actggaacaa cgtcttcagg
    62761 atttgcagca tctggtttgg tcacagaaat cgttccttct gtagtggctg agctcttatt
    62821 ctcagcaaaa acactttgtg tacataataa tagacttcca gctagaacgg tacttaatgc
    62881 aattgttttt ttcatttctt tttccttctt tcttttaaaa taaataatcc agcaaataag
    62941 ccaataagaa tgactccaac aagtatcagc catcgccagt caaatgtttc tggctgattt
    63001 tttaaatcaa tatccttttc attcaattgg tccgctgcat catcagttat ttcaaaatcg
    63061 gtatggagat tccagcgttt ttctttccaa gcacccttca caaccgcttg ataatgtccc
    63121 ggagctaatt tttttccttc taatgggaca agtaaatcaa aatttgtatt gggtgctaca
    63181 tctatttttt ctttttttat tgaaaggtaa ggctcttttg tttctttttt gtagatttca
    63241 accgatgtag tcaagccgaa catataagaa gcatttggat tttgaagatt cgccttaatc
    63301 acatttcttc catcgctttg atccgcataa gcttttttta atttcaaatc tggggaagac
    63361 tctttttcgc tatttcaaat caagattgct ttcacttgag aaaactgatt gacgacacca
    63421 actgtacttt tgttagaaac gttctctttt ttttcttgaa acgtgatccc tcctgcgacc
    63481 acaccagcca gctctttttc tggcgctttc agtgaaagtg tctcagtgac cgcactttga
    63541 ggaggaacag ttatttctcc cactgttttt gctaagtttt tcatatcata acttaatgtt
    63601 tcatcaaatt tactttttga ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg
    63661 gagtggatca atgtctcaac tgttactgat ttatctgttc ggttaaagac cgtgtagggg
    63721 atttcctgtg ttttcccagg ctccatcctt acatttatat atgttttgtt cttatcaatc
    63781 tgattagccg gtaattcaaa atctaccgca aaattgaatt cggaggcatg gacaggtgtc
    63841 tgccaaagga tgaatccaag gagaaggatc ataagtaaag agcagttgtt tcgatgactt
    63901 ttattccata ttcgattcat caatcatcct ccttgatctt tattctgtcg gcgcatctgc
    63961 taatgtccat gttatctctg ctgagtaatt accagctatc tgattttctt tggttaagac
    64021 aagtttaaac gcccgaccgt tatttaaata ttctgaataa tctgcttgca cattcgttga
    64081 attttgtcct ctgacaattt ccagttcttc ttcattcact aattgatact cctctccttt
    64141 cttgaaatat aggacatcct gcatagaaaa tcctgcatca tttgtgaaag gctgagataa
    64201 ttttatcgtt aagatccacg gatttttcgt ttctcgctga tcataaatcc ataatttttc
    64261 ctgtatatcc atttggccaa atgcttcttg tgtcaaacca gttgctttga tctgaccaaa
    64321 atcgagtttg gaaggggcaa gaaatgagag accaccttct actacgatct tcgtagcagg
    64381 tgcaaaaggc ggggcatcat gataaacaac tggcgtatca cttggattgt gattcccaat
    64441 ttcatcgttg gtacttggct tgttctgcac acccttagca gtagcttctc ctgcttggtc
    64501 gtttaataca atttctaata catctcctgc aaaaagttga ttttctggga tagaaagaac
    64561 aaattcccct gtatcatcaa tagttgtttc tgacaaaaca gcttgatttt ttttcaatga
    64621 aaccaaggct cctgcttcac ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt
    64681 ggtacttaaa ttagcaggtt caggaggaat cacatcgact gtcgttttgg cagagtccag
    64741 ttctgtcact tcttggccac ctcttacttt tttagcagca acaacttgca cttgatcctc
    64801 cgtttttaat ctctcatcat tgggtacttg aacttcgaac aagccgcctt gttcgccttc
    64861 tccccatggg ttatatcctg ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt
    64921 aatttctaca gatgatttcg tcgtatctgc gtaagtaata cgtaaagtca cttctacttc
    64981 gcctgtccaa gcatctcttg gctttgcctc gacaccttcg ggaacaacga catgcccata
    65041 gattttttta tctgcattcg ttggcactct taaggattca atcatcggga gctgattatt
    65101 ggcactgatt cgattataat ttttcattcc ggcacctgtc tgagcaaatt catttgttaa
    65161 acctgcacta gggcttgata cgaaattgcc taagtcggtc ccattgatcg ataagtctgt
    65221 catatcagaa aaataatccg ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg
    65281 ccataaagcg atttctgaat gggcgatctt taaagaagaa tctccatttc cttggaatac
    65341 ccagccgcct atgccattgg tctcgcttcc aatccgtttg ttttggaaat caaaataggt
    65401 aggagattct aagtccacat tcaacacatc cccactgatg atgccggtct tatcttcccc
    65461 tgttttcccg accatgacta aataagtttt ttcacctgcc gtaaaattta aatcaatatc
    65521 tccagttgta tctacagttg ctccggaatc tgcgcgtata tcgatatttg atttttctcc
    65581 agtgactgta aaatcagctg aacctggact ttctagaaaa ccagaagcgt cataaaattg
    65641 gatcgcttga tttccttgat tgagtctgtt cacgtaagat ttgccatcac ctgtatttct
    65701 taccctcaat gagcctccat tggttactgt cagcttttgc gtaccattct caaaacgaac
    65761 tgcgctggaa cgcccactct gcttatcgat gtcgataact gatccggctc cttctacgat
    65821 caaagaaagg ccttttccta caaaacgcat cccattgttc ccgggttgat cgtcatctcc
    65881 agccattttg accgagattt gtccaccact tgatacatgg atttgactag aatctccttg
    65941 caagcgaaat ccgcccgtag ttgctgtatt gatggtgatt ttcccaccat tcgatacagt
    66001 gactttcgat cctgaacctc tcatttggat gattccgcga ttgttgtcat tggtaatatt
    66061 gatctttgta tttaccgaaa attcgcttcc cgaaccactg acttcaaaca tgacattacc
    66121 aggtagcgta tcgactgcct ctccttcatc gctaacgcca tctgcccgat cataatcaag
    66181 agagaccttt gaaccgctta agatatggat tcctggcgct gtttttccac taccagtctc
    66241 atctcctgaa tctagaaaac cgtagaattt ccctagatac ggtccataaa gttgactacc
    66301 attgatcaca ttcaattgat agcttctaca aaaataggct ttacccggag cggcattcag
    66361 tccttccttc actggttgga catccaacac accggaaaca ccatctaatg tgacagttgc
    66421 acgaggagcg taaatcagtc ctaatttgga cccatcatac aaatcaagtg tcccggtaaa
    66481 ttttatttct cccgtaaaat gatatctgct tccccaaaaa tcggtcatat aagcaatgat
    66541 aatggcattt ccatcgatat ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa
    66601 attctggaac tcgatcgact gaggtatctc attgatataa atatggtgtg tgccgaaatt
    66661 gtgagcgaat ccaccaaaat caataacgat atctcgtttg atctctaatt ccctgtctaa
    66721 agtcaaaggc tgaatcagtc ggatcttagt tactgattcg tctgcgagaa gctgcgccag
    66781 ttcttctgtt gtggcagctt cgctgtaccc atccgctgaa acagctcttg ccagtgccgc
    66841 ttcactgcta tcagacgttt gttcagctac ttctgaggtc tcacttgtct tattttcttc
    66901 ttgcctttta ttagtagaag cctcactttg agattctgta gtttcagaag taatcgattc
    66961 ttctgaaaaa gtagtcttgt caaaactttg attctctgat tgcgttgatt cgtttgtttt
    67021 ctctacatcg gcaaaaacgg atgtcaccat caagagaggg aagacaaaca aaatcgacgc
    67081 aatcagcttt tttacacatt gattcattct ccaccatcct ctctgacgtt ttgacaattt
    67141 tattttatcg ttttaatcta tttccaattg tactttcata tttaaaatat gtatttttat
    67201 ggaaaaattt ttctattagg tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa
    67261 agtcttgtca cagtccctta tcccgaacaa gcggtgttct cccgccaact cctcggtcac
    67321 aacctccatt ttaacaataa acctgtaaat gtgagggcaa tactttgatt gtcacaggta
    67381 atttatcccc ttcatctcca tcaacattcg tcgtcaattc ttcctcatca gctaatgaca
    67441 caactaattg ctcacaagta aggtaggcta gatttttcgt agattcatct actcctttta
    67501 ataggtctgg caatgtttta agcgtatcga tcaaagaaga atcttttaaa taaaccaaat
    67561 ggagcttacc atcatccact tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt
    67621 tagttagtcc tatcagcaag gtactgctct caatttcttc cttctttcca tcaacttcta
    67681 cgtgaaacga gtaggattgt gtggaagcta actgtttgat tcctgacatg aaataagcta
    67741 gttttccaaa tttcgtcttc ttctccgcat ctacatcatt gattgattca gggatagagc
    67801 caattgcgac gacattcata aaatagtcgt catttatttt tccaatatcc aatggcttga
    67861 ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc tagcggtatc cctaatgctc
    67921 gagccaagtc attcaccgta cccaatggga aaaatccgaa atttggccga tgttcttgtt
    67981 cagcgattcc actgattcct tcgttgactg ttccatctcc gcccatgaca aacacactat
    68041 gatacccttc cgtagccgct tcgcgagtaa agttttttgc atcccctgct ttttttgtat
    68101 gcaagacgac cacttcatca aacaccgatt ccaattttgc tatcgctaat tgctcaaatt
    68161 cctttgcttg ttcaccgcct gaactggggt ttaccactaa taaaactttt ttcatcactt
    68221 tctttccctt ctgattgatt tctttaatat aacgatagca taaagaaaaa aattaggctg
    68281 tgatgaagta ctgttcatga aacattcatt tattttttga caagataaaa catccatact
    68341 atgaaactga ggaaaattaa aacaatgata taggaaataa aagaaaatga taggcattgt
    68401 atacctaata aagtaaaaat cttaggtggc agataaaaac acaaaaatag caagagaatg
    68461 taatagcgtt tcttccactt tttgttatca gaccttatag cgatcagagg aataccaaat
    68521 gcataactga aaaactcttg atgtactgtt aatatgtcac ggccttgaat aataaatgcg
    68581 acaatccccg ctataattag tgtgcctaac accaaaacgg aaaattgatg aatatatttt
    68641 atatacaaat gatccattgt acttcctgcc tttttatttg tgagaaaaac aaatccgtat
    68701 acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc actccattta tctacttcta
    68761 cgtgcatttt ttgatattgt tttctttatt taagattata cagaaaaata tctagggtcg
    68821 ctggattcta agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt ag
//
LOCUS       DACEJR010000008        56295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEJR010000008 DACEJR010000000
VERSION     DACEJR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.008"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..189
                     /locus_tag="IW651_000550"
     CDS             <1..189
                     /locus_tag="IW651_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0813303.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IW651_000551"
     CDS             complement(1055..1585)
                     /locus_tag="IW651_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ0813304.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IW651_000552"
     CDS             complement(1582..2199)
                     /locus_tag="IW651_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0813305.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IW651_000553"
     CDS             complement(2781..3002)
                     /locus_tag="IW651_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ0813306.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IW651_000554"
     CDS             complement(3180..4712)
                     /locus_tag="IW651_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0813307.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IW651_000555"
     CDS             complement(4848..5933)
                     /locus_tag="IW651_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0813308.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IW651_000556"
     CDS             complement(5948..6667)
                     /locus_tag="IW651_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0813309.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IW651_000557"
     CDS             complement(6664..7974)
                     /locus_tag="IW651_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0813310.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IW651_000558"
     CDS             complement(8001..8591)
                     /locus_tag="IW651_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ0813311.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IW651_000559"
     CDS             complement(8909..9970)
                     /locus_tag="IW651_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0813312.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IW651_000560"
     CDS             complement(10538..11605)
                     /locus_tag="IW651_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0813313.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IW651_000561"
     CDS             complement(11625..12104)
                     /locus_tag="IW651_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ0813314.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12581)
                     /locus_tag="IW651_000562"
                     /pseudo
     CDS             complement(12121..12581)
                     /locus_tag="IW651_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            complement(12595..13845)
                     /locus_tag="IW651_000563"
     CDS             complement(12595..13845)
                     /locus_tag="IW651_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0813315.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14223..15674)
                     /locus_tag="IW651_000564"
     CDS             complement(14223..15674)
                     /locus_tag="IW651_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0813316.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15995..16759)
                     /locus_tag="IW651_000565"
     CDS             complement(15995..16759)
                     /locus_tag="IW651_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0813317.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16787..17485)
                     /locus_tag="IW651_000566"
     CDS             complement(16787..17485)
                     /locus_tag="IW651_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0813318.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17497..18276)
                     /locus_tag="IW651_000567"
     CDS             complement(17497..18276)
                     /locus_tag="IW651_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0813319.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18292..19236)
                     /locus_tag="IW651_000568"
     CDS             complement(18292..19236)
                     /locus_tag="IW651_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0813320.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19282..20061)
                     /locus_tag="IW651_000569"
     CDS             complement(19282..20061)
                     /locus_tag="IW651_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813321.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20441..22516)
                     /locus_tag="IW651_000570"
     CDS             complement(20441..22516)
                     /locus_tag="IW651_000570"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0813322.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22518..23435)
                     /gene="glyQ"
                     /locus_tag="IW651_000571"
     CDS             complement(22518..23435)
                     /gene="glyQ"
                     /locus_tag="IW651_000571"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0813323.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23821..24639)
                     /gene="recO"
                     /locus_tag="IW651_000572"
     CDS             complement(23821..24639)
                     /gene="recO"
                     /locus_tag="IW651_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0813324.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24782..25681)
                     /locus_tag="IW651_000573"
     CDS             complement(24782..25681)
                     /locus_tag="IW651_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0813325.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25696..26097)
                     /locus_tag="IW651_000574"
     CDS             complement(25696..26097)
                     /locus_tag="IW651_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0813326.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26075..26551)
                     /gene="ybeY"
                     /locus_tag="IW651_000575"
     CDS             complement(26075..26551)
                     /gene="ybeY"
                     /locus_tag="IW651_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0813327.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26580..28772)
                     /locus_tag="IW651_000576"
     CDS             complement(26580..28772)
                     /locus_tag="IW651_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0813328.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28796..29767)
                     /locus_tag="IW651_000577"
     CDS             complement(28796..29767)
                     /locus_tag="IW651_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0813329.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30472..31851
                     /gene="brnQ"
                     /locus_tag="IW651_000578"
     CDS             30472..31851
                     /gene="brnQ"
                     /locus_tag="IW651_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0813330.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32007..32453)
                     /locus_tag="IW651_000579"
     CDS             complement(32007..32453)
                     /locus_tag="IW651_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0813331.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32480..32656)
                     /locus_tag="IW651_000580"
     CDS             complement(32480..32656)
                     /locus_tag="IW651_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0813332.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32818..33246)
                     /locus_tag="IW651_000581"
     CDS             complement(32818..33246)
                     /locus_tag="IW651_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0813333.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33338..34198
                     /locus_tag="IW651_000582"
     CDS             33338..34198
                     /locus_tag="IW651_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0813334.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34292..34585)
                     /locus_tag="IW651_000583"
     CDS             complement(34292..34585)
                     /locus_tag="IW651_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0813335.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(34658..36385)
                     /locus_tag="IW651_000584"
     CDS             complement(34658..36385)
                     /locus_tag="IW651_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0813336.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36598..37524)
                     /gene="trxB"
                     /locus_tag="IW651_000585"
     CDS             complement(36598..37524)
                     /gene="trxB"
                     /locus_tag="IW651_000585"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0813337.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37669..39102)
                     /gene="ascB"
                     /locus_tag="IW651_000586"
     CDS             complement(37669..39102)
                     /gene="ascB"
                     /locus_tag="IW651_000586"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0813338.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39102..40505)
                     /locus_tag="IW651_000587"
     CDS             complement(39102..40505)
                     /locus_tag="IW651_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0813339.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40735..41637
                     /locus_tag="IW651_000588"
     CDS             40735..41637
                     /locus_tag="IW651_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0813340.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41678..43564)
                     /locus_tag="IW651_000589"
     CDS             complement(41678..43564)
                     /locus_tag="IW651_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0813341.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43661..44513)
                     /locus_tag="IW651_000590"
                     /pseudo
     CDS             complement(43661..44513)
                     /locus_tag="IW651_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44848..46632)
                     /locus_tag="IW651_000591"
     CDS             complement(44848..46632)
                     /locus_tag="IW651_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813342.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46632..48383)
                     /locus_tag="IW651_000592"
     CDS             complement(46632..48383)
                     /locus_tag="IW651_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0813343.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48525..48749)
                     /locus_tag="IW651_000593"
     CDS             complement(48525..48749)
                     /locus_tag="IW651_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0813344.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49000..50586
                     /locus_tag="IW651_000594"
     CDS             49000..50586
                     /locus_tag="IW651_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0813345.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50652..52427)
                     /locus_tag="IW651_000595"
     CDS             complement(50652..52427)
                     /locus_tag="IW651_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0813346.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52638..53540)
                     /locus_tag="IW651_000596"
     CDS             complement(52638..53540)
                     /locus_tag="IW651_000596"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0813347.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53819..56164
                     /locus_tag="IW651_000597"
     CDS             53819..56164
                     /locus_tag="IW651_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0813348.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16683 a        11715 c         9117 g        18780 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaacttttc ttttctattt
    12421 acttgtctca tgtagtacgt tttttcgcct tttgactcag ctgaatattc tgtctgttct
    12481 gttataacaa aactctcata cttctccatc agtggtttta acatcattaa ctgttcataa
    12541 tgccccccag aggaagccgc aaaacatatc tttttgttca tattaacctc tctatcagtc
    12601 acgctgaaaa atatctctag tgtaaacttt ttctttaata tcatccaagc aggtatcata
    12661 tcgattggca ataatagaat cacacatcgc cttaaactgc tctaaatcgt tgataacctt
    12721 acttccgaaa aaagtctctc catctttcag tgcaggctca taaatcacca cagtcgcacc
    12781 tttagctttt acgcgcttca taactccctg gattgaactc tgacgaaaat tatcactgtt
    12841 gctcttcatg gtcagccggt atataccgat taaaactgga ttttccctgt tgacatcata
    12901 ctgattatat tcagcgtagt tgtaatagcc cgccatctga agaacacgat ctgcgatgaa
    12961 atccttacgg gtacgattag attcaacgat agcctcaatc aaatttccag gaacatctgc
    13021 atagtttgcc aacaactgct tggtatcttt tggcaagcaa taacctccat atccaaagga
    13081 tggattgttg tagtgagttc caatacgagg atcgagacaa acaccattga ttatctgctg
    13141 agtattcagc cccttcattt cagcataagt gtctaattca ttgaaatagc tgactcgcag
    13201 tgctaaatag gtatttgcaa acagctttac tgcttcggct tcagtgaatc ccataaaaag
    13261 agtatccacg ttctccttaa ttgcactttt ctgcagaagt gttgcaaaaa catgagcagc
    13321 atttaacaag cgctcatcag tcatatcagt accaacaata atacgagatg gatacagatt
    13381 atcatataaa gccttgctct cgcgcagaaa ctctggacta aaaataatgt tcttactccc
    13441 cgttgtctca cggactgatt cagtgtagcc aacaggaatt gtagacttga tgaccatgat
    13501 ggtatcagga ttcacgtcca tcacaacctc aataactgac tcaacagcag aagtatcgaa
    13561 atgctgagta cgactgtcat agttagtcgg tgtagcaata acaacgaaat ctgcggattt
    13621 ataagcgctc cttgcatcca aagtagcagt taagtcaagt tctttttctg taagatactt
    13681 ttcaatatat tcatcctgaa ttggagactt ccgtttatta atcagttcaa tcttttcagg
    13741 gataatgtct accgcggtta cctggtgatg ctgcgccaat agtgtggcga tactcaaacc
    13801 aacataaccg gtgccggcta ctgcgatgtt gtactttttc ttcacaatat ctcctctttc
    13861 ctctcttaag tatggagaat gttactccta cggctattca tcatgtcaaa ataacacagc
    13921 ttagaatgtt gataaaataa ttttccagaa tgtgataaaa tctctttcat ttataaacca
    13981 cacatctaat tttatctatt ctccacaaga catgtagagt attctttaat aacaagatac
    14041 gaccttacta aatacttcaa aaaattatca tatttaattc ataaatataa atcttcattt
    14101 taaatgtatg cccaaaaatt tatctgtaat gcgtcatcaa aatctagatt ccaaatcttt
    14161 atcaattttt atccactcat tctaccactt tagtaatatt ttctatttat aacactttcg
    14221 tcctacattg atccccccct ctgaagtaca gtttttactg tcttaaataa aattttaaaa
    14281 tctaacccca tactccaatt ttttatatat tccgtgtcaa gttttgctac ttcttcaaaa
    14341 tcagtaatat cacttctacc acttacctgc cacattccag taattcctgg cttgatagca
    14401 attcttgcac gatgatgata atcatattct tcatactctg atttaaggca tggtcgaaat
    14461 cctataaggc tcatctttcc tgttagaacg ttgaaaaatt gagggaattc atctaaagac
    14521 gtggatctaa taaattgacc gattcctgtc ttttttgttc catctggaag tattttattt
    14581 ccgataattc ttggatcaaa ctccatctta aacataagat tattgctcat cttattttct
    14641 ttcattaatt ctacttttct ttcttctgca tccatataca tacttctaaa cttatacatc
    14701 ttgaacggtt taccattctg acctatacgt tcttgtgcaa agaaaattgg gcctggtgaa
    14761 ttaatataaa taagtggagc aataaagata aaaataattc ccgttgcaat acatccaaca
    14821 atacctgcaa gaatatcaat tgttcgtttt ataaatgcct gttgagttgt cataacattg
    14881 atacttgttg taagtactgt atattctcct atacgaccaa tagattgttt tcccaaagca
    14941 ctatctgata cttttgcaag attcaaatgt acagtaaggc ccatttcaga gcatcgttca
    15001 ataaattctt tcatacacga agtactttta ttcaaaataa taaagacttc atctacccat
    15061 ccctgacata agtaatccgc tgcatttttg gaatcagaaa caactgtcac tccctcaaca
    15121 tcttcaccaa tcatgctttt atctatgata atgacaccat ttattttata cattttatag
    15181 ttatgctctt tcacattacg aataactact tcagcaatac tactagatgt aactataata
    15241 agtgatttgt tcccctcatt tttcatttta tgtttcaagt aatgtttcca taagaggcga
    15301 gttatatatg taattacaac gtataaacct cccatcaaga aaagaacgac tctagaataa
    15361 tcatttccac cctgaacact aatgagatac aaacttccta acagcaccaa cataatcgct
    15421 tgctttacag aagaagcaaa ttctatatag taccctcttt ttaatacacc tctatatgct
    15481 tcaaacatga aaataactaa aagatctgcc agttcaatga agactgccat gttttgatac
    15541 attggaatca cataaggatt ccaatctacc tggcgtaaga catacgatag ataaaaagca
    15601 agctggatac acaataaatc taaaataata aaatcaatat gtttaaccca tccccttgaa
    15661 cttttcttgt acattccttt ttactcccct ctttttaata aaataaattg tattacttgt
    15721 tttctctcct aaatatcttt ctgaaggatt attgactcga caaattggct tgtggatatt
    15781 cttatatata gaacatccgt aagccaactt aaaactcctt aacaaaattt ttttactcat
    15841 attgtagatc aaaacaagta tccaagaaat catattttat ttcttttttc tgcttactca
    15901 acaacagcta aaagaaaaat catcataaac aaattcaact aagccaacga tccccttgtg
    15961 aaatcaagtt ttttcttgat ttacgaataa agagctaaaa taaaccaaac ttcttctttt
    16021 ttacctcttc atagactgga taactgattt catccccgtt gatcagatcc tttgcgactt
    16081 gtttcatctg tactactttt tccttaccaa agtcctttgc aatctgctca tagcactctt
    16141 ttaagtaaaa ggtgcgctta gagaccccat gggcatcaga agcagccatc tgcacgagat
    16201 tatgtttgac catcgttttg gctgttttct ggatgtcttt gccaaatacc ccaacataac
    16261 ttggcgcagt gagttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt
    16321 cttcgcgaaa atgcacgttg cgttctggat ggacgattac tggtgtcttt cctagttcta
    16381 acaactcaaa aaaaagccgt tctgcatata acggcacttc cagtgtcgga aattcgatca
    16441 gaagatatgt atcatctaaa tctgtaaata atatttcatc tcggtggatg tcttcgatga
    16501 gcgttccggt aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct
    16561 ctaattcggc ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt
    16621 ttccattatt gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt
    16681 cagccattgc aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat
    16741 ggcaatgtag atcgatcatt tctcttgtct cctctcttct tattccttaa tttccgtagt
    16801 aataaccagc atctttgcta tgttctgctc cgttgtacac aacacctaag actcgtgctt
    16861 gtaccatctc cagcagttct tttgctttag tcaaggattc tttacgtgac atattttcac
    16921 gtacgaccaa taaagtgcca tccgttttta aagacatgat ctgtgcatct gttacggcta
    16981 ctactggtgg catatcgaat atcaccacgt caaataactg tcttgcttcg gataggatct
    17041 gatccattct tggcgagctt aacaattctg atgggttggg tggcttaggt ccactaggca
    17101 aaatcgacaa gttctcgact ggcgttcgtt ggatcacatc tgctacactt cctgatgaac
    17161 taagtgctgt acttaatcca ctggcattgt tcagctggaa tgtcttatag acgacgggtt
    17221 tacgtaaatc tgcatcgact agtagaactc gttgacctga tttagcaaaa acgactgcaa
    17281 tgtttgctgc agtggtggat ttcccttctc caggacctga tgaagtcacg acaatcgttt
    17341 tgatttgctg accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt
    17401 cggcgattgg tgaagatggg tttgttaatg tgatcaaact gactgcatga gtttgtgctg
    17461 ctttttgttt ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg
    17521 tgatggggct gttttatcgt ttgtagcttt tggtcgtgca ggagctgttc cttttttacg
    17581 gataccagcg ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc
    17641 taatgtatct gtcacatatt tttcatcttt cactgtccga tcgagtaatt ccaataagaa
    17701 tgccagacct acacctacca tcattcctaa cattaagcca gtcattaaat tcaatttatt
    17761 atttggcgaa acaggtgtcg tattggcaac tgcacctgaa atgatcgaga ttttatcaac
    17821 attcatcaca tcttttgcgt tctcttggaa aacttgtgaa gttgtgttag cgatctgttg
    17881 agcagccact gcattcgtac ttattgcctg aatcgagaac atttgtgaat tttctgcctg
    17941 attaactgag atggcatctt tgatttgttc agcagtcatt ttcacattgt cttctgtttc
    18001 taaacgatct ttgacttcat tgagcactaa atcactgacg atcatatctt tatacgtatt
    18061 gatcatttgc aggttcgtat tgacatcatt gacatttgca gtgtctgatt gtggcagtgt
    18121 cacgatcaat tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc
    18181 tgagatggcc aagccagcaa acattgagat gatgattatt accaagtgtt tgcgtaatgt
    18241 ttcaaaaatt tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt
    18301 ctctagattc atttgatctt ttaattcttt tttcacacga tcaagttctt gatcactgac
    18361 tttttgatag gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttaat
    18421 cgtaccaaat gcctcccgat aatctaaagc aatttttttc atttcatcaa agttcatgtc
    18481 tgttttcata ttcgactcta gagcattcaa aacggattga tagttgctga caccgtttag
    18541 actcaatact tttttcgcta cgccttctac gatctttctt tggcgccctt gtctaccgta
    18601 atcaccattt gggtcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa
    18661 tgtaatttta ccgattggaa agtcatagcc atcttgtgag aaagtgaggt cattattgac
    18721 ttcgatacca ccaactgcat caacgagttc ctttagtcct ttcatattga tagagacata
    18781 gtgatcgatt gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc
    18841 gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt
    18901 atctcgagga atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat
    18961 catcgtatct gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa
    19021 tagaacagag aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt
    19081 tcgttccact gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc
    19141 tcctactcct acaattgcta atactaatac gataagaact gctaaaataa tcaaaaatat
    19201 ttttttccct cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg
    19261 cttgaatcaa cgttttaact gtcaatagct tatattgcta tagcccattt gctgagcaac
    19321 agtttgtgct tttgttgcgt atgcggctct tattttttca ttccggatct gctctatttg
    19381 actgagcgcc gtcaaatatt gatcgtaagt agcggtagct gttacttgac cgtctttcag
    19441 catcgtatcg aatagctgat ctagttgcgt gacttgtgca atttgggtat cagcgaatcc
    19501 tagatattgc ataacgactg ttttccaagc actatcctta aaaaagctca tattttcacg
    19561 tacatgggcg acatctgcac ttgccaactt ttctttgatg atgacatcat ccacagcttg
    19621 ctgccagttt gatacgtttt tagtaaataa tttattgaca gccgtctgga tcttcagttt
    19681 tgattctgca tcctctaact gcgttaagac tgctttcttt tcttcttgga tcgcagcagc
    19741 ttctttaggt aattctgatt ccttgatgtc ataatcatct gccgaaagtt tgatagaaga
    19801 tagtttatgt gacaaatttg aaactgtttc ttctgttatt tcaggattta ggaaaatttt
    19861 ttcttttgta ctgtacagct tatctacttc ttttgataaa gaggtcacgg tgtctttttg
    19921 attggtgatg gcagattctg cgtaattgat ccgttgatga tattgccgct gattattgag
    19981 atggtatcca aacaagcaaa tcacaatagc gcctgtgcct accgccacag tagccaataa
    20041 cttgacgaac ggcttttcca tagattcact cctaaatatt gatgatagat aagtaaagat
    20101 gtgattcatt cacatcagaa tgagcgcttt ttattttaaa agcataatta aactaatata
    20161 ttcatagaaa tatttataaa caggttttcc ctatataatc ataccacata cagttttttt
    20221 gactagggaa attcctaatt taaaagtaaa taaaacagta acaagattat aattaatcgt
    20281 tttcattttt atttcctcac ttttcatgag cgttttgaaa attcgaacaa agaataaaat
    20341 aacaaaaatt attaaaatag aattaataac agctatttat aacaaaaaaa acagagacac
    20401 agcacgtttc gtgcatgctc tctgtttttc aaacaaaaga ctattttgtg atgatttgat
    20461 ccaagcttgc taatgacaaa gccatggcag ctatttgact tagttgtgtt agtcggttct
    20521 tacgtactgc tggttcatca gccatgacca tcgtttgatc aaaataattt tcgatcagtg
    20581 gacgaagagc gacaagctgg tcgtagtttt cgtggatcgt ttggtcagca aaacttgctt
    20641 tcattgcttc tactgctttg tgtaattctt tctctgcgtc gttttcgaac agctcagggt
    20701 ctacttctga ttgtttttca agctgcgctt ttttcgctaa gttcacgaca cgtgtcaaag
    20761 cttccattga aggtttgaag tcttcatctg caagatgttg tttgaacaat tgtgctgctg
    20821 caaatacttt tgtcagatct ttttgttccg cattcaaaac agcatcgatg acatcatgac
    20881 gaatattctt cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacttgtt
    20941 gttgcccttc tgataaacca atgccgaaac gatccgtatc tttactgata acatcgctaa
    21001 tcgtttcttg cagaacagac aaaggaaagg cccatttttt cgattcgatg atccgaacga
    21061 caccgtatgc ttggcgacgt aaggcataag gatcgtttga cccgcttgga atcatcccaa
    21121 ctgcaaagaa agaaaagatg ctatctaatt tatctgcaat cgctaaaaca gaacctactg
    21181 tagactgagg tagctctcct tcactggata taggcatgta atgctcacga atcgctgtcg
    21241 ctacagcagg tttctcgcct ttcagaagag catatttttc gcccatgatc ccttgtagtt
    21301 caggaaattc ccctaccatg ttggttacta gatcaaattt atagatttca gcagctcgtt
    21361 gcaaatccat caattcttcc tcagatagtc ctacttgttc gccaatgata cgggcaatga
    21421 cttgtacccg ttgcattttc tcatagatac taccgatttt ttcatggaaa gtaacagttt
    21481 ttagtttttc aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg
    21541 catcttctaa acgagccgtc aatacttttt gattgcctcg aaccacatct tccaatttta
    21601 cccgatcacc attacgagca gaaataaaat gaggaagcag cataccttga tcattccgta
    21661 cctcgaaata acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagaaa
    21721 gatatttttc ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt
    21781 cttctagtaa atcttcgtct agatcaataa cccagtgatt tttttctgct aattcgtttg
    21841 cttgtgtaac gatcatttct tgtctttttt gcggagacgc aatgacgaat tgttcattca
    21901 atttttcttc atattctttt gcatgcgcaa atgtcacatc ttcgcctaag aaacgatgac
    21961 ctcgagaggc tctgcctgtt ttgacatcca agatttcgaa tgggattact tcgtcatcta
    22021 acaatgccac gatccagtga atagggcgga tatattcaaa atcgtattct gcccaatgca
    22081 tcgttacagg gaaagtcaaa cttttgatca cccctgctaa gcctgtcaat acttcttttg
    22141 cttccttacc atgagtgtat ttcgttacat agacatattc cacacctttt aattctctaa
    22201 aagtgatagc gtctgtcgtc agaccttgtc cgcggacaaa tccttccgct gctttcgtcc
    22261 agtttccatc tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcactgt
    22321 ccgcttgtcg ttctggaata tttgttactt tgattgctaa acggcgcggt gtggaaaatg
    22381 tttcgatcgt atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta
    22441 attgtttcat gcttggtgtg acaacatggg caggcatttc ttccagcccg atttcaagta
    22501 ataagtcttt tgccatctta ttcagcctcc ttcacggttt ctggatttgc tgtgttttta
    22561 ttcaacaaag ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga
    22621 gccatgttgc ggatgcgaga aagataaccg gcacgctctg ttacggatac tgcaccgcgg
    22681 gcgtctaata agttgaatgt atggctacat ttcaagatat aatcatacgc cggatgaaca
    22741 agattttcat cgatacagcg tttagcttct ttttcaaaac gatcaaagtt gcttagtagc
    22801 aagtcttggt cgcttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag
    22861 atctcgccat atttcacgcc atcagcccat tctaaatcat agacactttc aacttcttgg
    22921 atataagaag caagacgttc taatccataa gtgatctcag aagttactgg tttacaaggc
    22981 agtccaccaa cttgctggaa gtacgtgaat tgtgtgattt ccattccgtc aagccatact
    23041 tcccaaccca caccggcaca gcccatggat gggttctccc agttgtcttc aacaaaacgg
    23101 atgtcgtgtt ctaatggatc gattcctaaa aggcgcaagc tttctaaata aagctcttga
    23161 atattctcag gtgaaggttt catcaccact tggaattgat gatgctgata caaacggttt
    23221 gggttttcac cataacgtcc atcagctggt cggcgagatg gctctacgta agcagcattc
    23281 catggttctg gtccgattgc ccgtaaaaag gtatatgggc tcatcgtacc tgctcctttt
    23341 tctgtgtcat aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt
    23401 aaaatcatat cttggacggt caatttttta ctcatctgtt ttgccccttt caattttaaa
    23461 gcgttgctgt caaataagaa tacaggaaag aaaacgacaa aaaatcccta tgtctgatta
    23521 cagacatagg gacgaataat tcgcggttcc accctacttc cgaataaaga ttcggcagct
    23581 tcatttatcg tactcgcaag tgcctcttca aaaatcgttt gggtccggct cccactctcc
    23641 cgaactcgct aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca
    23701 gttttcttgt ttctataata gatcctatca ctgaacgatc tatttaatct atggttaata
    23761 atagacggaa ctccctgtgt ttgtcaaatg gttttgattt tttgttgtat gttttattta
    23821 ctattcttct ttttcaggtg tctctttttc tttttcagac atgtctttct ctgacgtctt
    23881 taatacgtgt gcccaagact tcatttgatt gatgaacttt ttgcttttca aatggatacc
    23941 gacatattca tcatacagct ggtcgatgag ttcacggatt gcttgtttgg tttcttcttt
    24001 cagatggatt ccactaattt tatcataaga aatagctgaa aacatccgaa cgaaataaac
    24061 agctcgagga tctgcatgat aacggtgatc gtccatcgac caatgtcttt cgcatatcac
    24121 accaccatat ttggaagaat agtcaaattt tccttttgtt tccccgcata cgacgcagtg
    24181 tgtccagatt ggtgcaattc cgaagcgttg cagtatttgg acttcaaaaa tattcgtgat
    24241 gatttctgca tcgtctcctt ggtccatccg ttgcagtgct tgttgcgtaa actggaacaa
    24301 atgcgggtcg tactgatgat cttcgattgc cgcatctact agattcaata aatatgtgcc
    24361 atagccgctc aaaaagatgt cttcttggat gtgtcgaaac gcatggacat ctttgacact
    24421 gcttaaaaat gacaaacctt cacttcgaaa attcccgata taaactgctt cagtaaaagg
    24481 gagcaaagcc ggagctaagg gctgattttg ccgatggatc tctttgatat aaaacatcat
    24541 tttgccagaa gattctgtaa agattttgac taatttatct ttttctttgt aattacggct
    24601 agataaaatc aagccttttg attctgcaag ctggctcata ttctccctcc taaaaaataa
    24661 atctggaaaa gcagattctg ccgatccaga tcatttatgt tgcgatcttc cgcagctttt
    24721 ctcaacacgc ggatttttat tcagtttatt tctataccta atttacaaga agctcagaat
    24781 atcagtattc gtcttttcgg tagccgaagt cttgcagata gacttttttg tctcgccagt
    24841 ctttttgaac tttgacccaa agttctagaa agactttgtc tcctaaaaga ttttcgatat
    24901 cttttcttgc tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgatcc
    24961 ctttttggct atctctttcg acaataatcg tagcctggat atggatttta tcattctcgt
    25021 ttcgcttcat cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa
    25081 acaagacttt ctcacggatc aattcagaaa cgatgaagta ttcaggatga tcagtcactt
    25141 gatcatctgg aaaatattgt gggccctctg gcatttcact gactaataca tccatcaaac
    25201 gttcgacgtt gtttccttct gtagcagaga ttggaatcac ttgtgcaaaa ggcatctgcg
    25261 ttgaataatc ttcgatgatt cccaacaact cgtctggatg gattttgtcg attttattga
    25321 taatcagata aacaggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc
    25381 gaccgcgttt ttgatcagca ctaatcataa ataagaccgc gtccacttct ttcaacgcac
    25441 tgtaagctgc ttctaccata aaatctccta aacgatgctt tggtttatgg attcctggtg
    25501 tatcaataaa aatcaactga gcttctggtg tagtgtatac tccttggatt ttatttctag
    25561 ttgtctgtgc cttgtcactc atgatggcaa ttttttgacc cacgattcga ttcaataatg
    25621 tggatttccc tacatttggg cggccgacga ttgcgacaaa ccctgattta tgttctggca
    25681 tatatgttct cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa
    25741 cccaacgatg acggtaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat
    25801 ttttttccca atcggatgaa aatgaaaatc cgtaaacatg tccacgacat tttcaaaaac
    25861 agtattgatg atctctacta tccatacgag aaaaacagct aaaagcagcc ataaccattc
    25921 tatccgatca agttggaaaa tcacgcccat aaacaaagcg aggattccga acgcaacatg
    25981 tttcctcatg ttgcgttctt cctcaaaaac agttttgact ccttggacgg caaattccac
    26041 ggacatcagg aaatgtttgt ttttctctac gtttttatct tttaagtcca taggcatcta
    26101 agatctcttt ctgtaaacca aacatttctt tctcatcctc tggtgtcata tggtcatacc
    26161 cgttgatatg caagaatcca tgaaccgcta aaaagccaag ttcccgatca tacgaatggc
    26221 cgtaagcttc tgcttgctct gcagcacgtt cagtagagat catgatgtct cccagtacac
    26281 gaggcaatgg attctctcca tcttcaaaaa tcactgggat ttcgtcttcg ccctcttctt
    26341 ccaaggcaaa actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta
    26401 cttggattgc tgcattgtcc atgaaagtaa cagacatttc tgtttcttct ggcaactcta
    26461 aatatgatgc tgcaaattgc agcaagtcgt cgatttcttt cattttctct tctgagactt
    26521 ggtttgtttc atccatgaag gtaatatcca ttatacatcg cttctttcta ttcttcttgc
    26581 tattcttctt gttcttcttt tatcttctca acttccgatt tcattttagg atatttgatc
    26641 cgagaatgga aggtactgct taaaccgctg atcaatgatt tttctacttt acggatttct
    26701 tttaacgtca atccactgtc gtccaattgt ccatcggaaa tacgctcttc aataagatta
    26761 tggacaaaac tttcgatttt ttcgctcgtt ggatggtcca ttgcccgtac tgccgcttca
    26821 caactgtcag ctatattgac gattcctgct tcacggcttt gaggttttgg tccaggataa
    26881 cggaattgct cttccgtaac ttccgggttg cgttcctttg ccttaacgta gaaataacgc
    26941 atcagcgtgg ttccatgatg ctgtctacaa atatcgatca ccatttgcgg catattataa
    27001 tcttccaata ttttggcacc atctgtcaca tgtccaaaga taatatgttt actgtcttct
    27061 ggcaataaga aattgtgcgg attttccgct cctgaaggca agttctcaac gaaaaagctg
    27121 gcgtgtttga tcttgccgat atcatgataa tagcaagcta ctcgagttag taaagaacgt
    27181 ccaccgattt ctgctacagc attagcactt agattggcta ccatcatgct atggtgatac
    27241 gtacctggtg cttcttccag caactgtttc aataaaggat gattgggatt gctgagttca
    27301 tttaatacga tcacactatc atcattgacc atcaattcaa tataggggtg cagacctatc
    27361 gtcagcaaga atgaaaacag tgcacctgtg aatccgcaga ctagcgttaa ccaagtcgtt
    27421 gcatccccaa agctcattcc ttggtagata atcaaaatga catccatcat cacaggaaaa
    27481 ataatgaccc acatagcagc ggataaccct tgttctgaga tccgtttgcg tctgacgatc
    27541 gttgccatga gaccagaaaa tacataggac atcaaaataa ctgtcagaaa gttcgtgcca
    27601 atcgaatcat aaaaaatgaa aagtgcggat actgcttgaa aaataccagc catgatccct
    27661 gctcttcgat tcaagaaaaa gcttaaaaca agtggagcaa aagccgcggg gtaaaacagc
    27721 ggaatataag cagcttgttc tgtttggaag agttggaaaa atttcattag tacgacgctt
    27781 actgacattg ccgtcacgta gaaaagtacg tattttgttc tttctcctgc attttggaat
    27841 tgaagtgaat tataaatcag tacaccgatt tgaagcaaaa cagctaaaac catggcaatg
    27901 agtgggaaaa tagaagtcgt ttgattcgtc aatcctagta attccaattt tttcatcgct
    27961 gaagcatcaa tttgatttcc ttcgcgaaca ataatttctc cttggaagat catcactggt
    28021 tgtacagctt ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt caatacatca
    28081 ttaactacga tgccttgatc cactaagtag cgaatcattt gttgctgctc tgttgtgaca
    28141 ttcaaatatt gaatctgatc ttctgcttct tgtttaaaag cttccaattc gctttcacga
    28201 acttgtttac tcatctgttc gtcaatgagc ttcaaacttt catccctaac tgtgtgaatt
    28261 tccgtttcag tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac
    28321 gcaacgtttt ctgcattcac gttttcaaaa tttttcttca gtgtagcaat ccgctcttct
    28381 gtagtaggtt tcgggatatt ttcatcttcc ttcgccttat ctaccttgcc attataagat
    28441 ttgttgatgg tatcattcgt cttatcgatc aattcgaata attgcttgat ccgattgttc
    28501 tggtcatctg ccagatcttt ttgataagta tattctggag tcactgcttc tgctgccagt
    28561 tttcttcttt gttcggtttc ttccgtgttc tcaatcgttt tattcgcacg gatactctct
    28621 tctgctactt ggccttctct gaaatcaatg gccttttgtt tgacactact aaatgtaacg
    28681 ccgcagacaa tgataaaaaa caagaacaga aggaaaggca atgctgcctt tccgagtttt
    28741 tcttgtagtt tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt
    28801 cttgatgaag atccgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa
    28861 caacgtcact tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt aatgttcgtt
    28921 ctgcatgaac aagaccactt gtcgtcccac gaggcaagtc tatctgactt gtatccccat
    28981 tgacaatcat ttttgattga taaccgagac gggtcaagaa cattttcatc tgagcgactg
    29041 tcgtgttttg tgcttcgtcc aagataacga atgcatcatc caatgtccgc ccccgcatgt
    29101 aagctaaagg tgcgatttcg atcacgcctc gttccatcaa tcggttcgta tgatccaatc
    29161 caaaaatttg gtataaagca tcataaacag gccgcagata aggatccact ttttctttca
    29221 gatccccggg tagaaagcct aggttttccc ctgcctccac agcgggccgc gtcaaaatga
    29281 ttttttgcac ttcgcctttt tttagggcag caattgctaa tactacggca agaaacgttt
    29341 tccctgttcc ggctggccct acaccaaata cgatgtcatg tttggctacc gattcaacgt
    29401 atttcttttg accgctgttt tttacccgta taggttttcc attacggtct ttgacgatct
    29461 cttcctcata catctcaaca aaataatcta atgtaccttt ttgccccatt ttcaatgccg
    29521 tcacaacatc tggcgtactt acgtggattc ctcgtttgat caattcctgt aatgtccgaa
    29581 tgactgaggc agccaaggaa atctgggatt gctcaccaat gatctgaatg atttctccac
    29641 gtgtatggat ggttgtttcc gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac
    29701 ctaaaagcat gctgacatca tcagcagctg tcaatctaat ctctaaagaa ctagactctt
    29761 ccgtcaaaaa tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag
    29821 aagaaactat atagcaaata agaaaaggtt gtaaaagagt cgcttaacca tacgtaccaa
    29881 gaaacgttaa aaaatagccg tttccattcg ctaaaggtct cgttttgata aaaagaagct
    29941 gagaatgtaa gttatatgaa agtggagaac aaaatatttc gtttcattca acaagtattc
    30001 tctaggtatt tttcctgttt caaaaaaaga aaacaaaata gttaaaatat tcttgcattt
    30061 caaaaaaaac aggtgtatta ttacgtcaac aaataaacat acagcaaagc gtcgaggaaa
    30121 acagtaggct attcaacgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa
    30181 tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct
    30241 ccattatcag gctagcggat aactttatca aaaagcgtcc gtgaatgaag aagctttttt
    30301 aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc
    30361 ttttttttat tttaaggtgg tggttttcat ggaatctgat attagtggca ttactaattg
    30421 ctgcaacgtt tatatcgtca atcaaaatga taattattgg aggaaaaatt tatggaagaa
    30481 acaaaaactt attcatggaa acaactattg atggtcagtt cacttatctt cgggatgttc
    30541 ttcgggtcag gtaatctaat ctttcctgtc cacttaggtc aaatggctgg cgcaaactgg
    30601 gtcagtgctg gcgtaggatt tgctatctca ggtgctcttt tcccattact agctatctta
    30661 gctgtagtag tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg
    30721 tatgctgcct tgttcttaat ccttgttcat ttgacgatcg gaccattttt cggtacacca
    30781 cggacagctg ctactgcttt tgaaatggct gctaaaccat ttttgcctga aaaatataat
    30841 tcattaggta tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgtc
    30901 catccaacac ggttggtcaa atacgtcgga aagtatctaa actccttgtt tcttgcatta
    30961 ttagcaatca tttttgtcgt ttctttattc catccaatgg gaaatctaaa tcaatctgct
    31021 gcagatactt atcaaacaaa cgctttaaca aatggtgtcc tagaaggtta taacacggtt
    31081 gatgcagtag ctttgcttgc cttaagtgtc acttttgtcc atgcagtccg tgaccttggt
    31141 ttcaaagata aaaaagtcac agaattaacg gcaaaagctg gtagcctgag cgttgcgcta
    31201 gaagtcatga tctattttgg acttgttctt ctcggtgcga tgagtttgaa taagatgtct
    31261 ttatcagaaa atggcggaac tgcactttct cagatcacta atttttattt aggtaacttc
    31321 ggcgctagct tcttaggagt gatggtcaca ttaggtgtct tcactaccgc tatgggactc
    31381 gttgtttctt ttgcgcaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg
    31441 ctgcggttaa cgactttcgt ctcttttgtc gttgcaaatg ctggtttaga caatatcatc
    31501 caatggtctt taccagtatt gatgcttttg tatccattgt cgcttgcgtt gatcttgctt
    31561 tccttaacag caaaattttt ccaaaagaca ccatttgtct atcaagtaac gatgttgttt
    31621 gctgctgttc ctgccgtatt ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa
    31681 agagttgtag caagcatgct cgaattttat catcatcacg ttccttttgc tgcattagga
    31741 cttggctgga tggtcccaac gttacttggt tacgctggca gcctactgtt ttattacgcc
    31801 tatcgcctat cagggtataa acaagaagca aatgaactac cagaagaata accaaatcaa
    31861 acacaattta acataagaaa tattaaacag gaaaaaacca gctagatgaa agaatcctat
    31921 tctttcgtct agctattttt tagttctctt ttttacgcgc acaaaaaaac tgcggacaat
    31981 gattgtccgc agtttaaatg ataaaattaa gattgtaata gttctttcac aatggcattt
    32041 acttggtttc catctgcttt tcctttgact ttaggcatga tcgcacccat gactttgcca
    32101 aattctttca ttgatgaagc accagtttgt gtgattgctt cttgaacaag ttgtcgaatt
    32161 tcttcgtcgg aaagttgttc cggcatgtat ttttcgacta tcacaatttc actttttact
    32221 ttttcagcta aatcgtcacg tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt
    32281 ttcatttcgc gagacaacac cgttaattct tcttcttctg tcaagtcgtg accttcttta
    32341 atctgctcgt tttgtataga tgacttgatc attcggatga cttgtaaaga ttccttatct
    32401 ttcgctttca tcgctgtttt catgtcatcg tttaatctac ttagtagtga cacactacca
    32461 actccaattc atcacagaac tagaattttt tgcgttttct agctgcttca gattttttct
    32521 tgcgtcttac acttggtttt tcgtagaatt cacgtttacg agattcttgt aaagtacccg
    32581 cttttgacac ggaacgtttg aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa
    32641 cgactgtttt tgacatgtta attccctccc tccgagctta aaaagtaacc gtaattgtta
    32701 ttgaaatata tctcaaaaaa caagactgtc ctgtaaatat tatatctaat gctgttttga
    32761 ccgtcaagga aatcttgctt ttttttaaaa ttatcaaatg ttattttatt taaatcctta
    32821 ttgacaattt tcacatcttc cgaaaatctc gaatctatgc ccttcgatcg tacatccacc
    32881 gagctgttct tcgaaataat tcattggaca catatggatt tctttcgttg ttccgcacac
    32941 cgtacagata aagtgatggt gatggccgat ttccccttga cagcaatgaa aacgaaactt
    33001 catctctcca tttaaatcgg tttcttccag taaatcgatt tctgcaaaat catggagatt
    33061 tcgataaatc gtatcgtaac ttactccttt gaattcctga ttcatatagt caaaaacttc
    33121 tttagcggat acgtagcgat tgcgcttcac taaataagtt aacaatgctt ctcttttttt
    33181 agtatattta aaaccgttat ttttcaatgt ttctagtgat tgttcaatca atgtctgttt
    33241 tttcatgact tattcctcca aatcaaaatg attacgaagt acacattcta gagtatggta
    33301 taataaacat aaaaaaaatg caagtgaggt tttatccatg tcttccgatt ctacagaaat
    33361 cattactttt tttgtcatct ctgactctgc tggagaaact gcgacaaaat tagcgcaagc
    33421 cactatggcc caatatccta ccgttgagtt caatttattc cgcagaacat ttgtgacaga
    33481 tgagaaaaca ttgatgcagg cactgcaaga tgcactaaaa gaaaaagccc ttgtcctaca
    33541 cacattgatc aatcaagaat tgatcgagat gacgcgttct ttttgtgaag aacatcaatt
    33601 atttagtttt gatgtgatga ctccgccgat atcagaaatc gagcgcttaa ctggtatcaa
    33661 gcccattcgg caacctggcg cccttcattt attgaatgag aactatttca aacggatcaa
    33721 agcaatggaa tttgctgtaa aatacgatga cgggaaagat cctcgtgggt ttctagaagc
    33781 agatgtcgtt ttgctaggcg tatccagaac ttccaaaacg cctttgagct tgtttttagc
    33841 taacaaaaat ttaaaggttg ccaatctacc tttgatccca caagcacata ttcccaaaca
    33901 gctttgggag atcgatccta aaaaaatcgt gggactgacg aataacccag atattttaaa
    33961 caatatccgt aaagaacgga tgcgctctta cggattaaat cctgatactg cttattccga
    34021 tatcgaaaaa atacgagctg aactcgattt tgcgaatgat ctttatgaaa aattgggctg
    34081 cgtagtaatc aatgtcgctt ctttatctat cgaagaaaca gcttcactta ttttgaatgc
    34141 tttagaccta gaagatcata gttattatgg aactgaaacc accgaagaaa cagactaaca
    34201 gtttttcttg tctttttctt aaaaaaactg gactgtgtca gcgaagaaga ttcgttgata
    34261 cagtccagtt tgtctctatt tgcacctatc gttattgatg ttccacatgt ttgagcgtct
    34321 ggttcaaaat atccagtacg tgagaatcat caagactata caaaatcgct tttccctcac
    34381 gatgggactt gacgacatgg ttttctctta ataaacgcag ttgatgcgat acagcagatt
    34441 gttccatttc tactgcttgg gtgattgctg tcacattttg ttccccgtct ttcagataca
    34501 gcagaatttt gagacgagtg gggtcactca acattttgaa tagttggctg actttctcta
    34561 cttttgtttt ttgcgtgtct ggcaatccat tcacctccaa cttgaaaaaa gactgacttc
    34621 taaagtttac cactttttcc atcagtctca agttttttta ttcttctgtc atttttttga
    34681 taatcgcttc ataagcggct actttttcag ctgcgtcttc tgaagagttt ccttttacac
    34741 cgatataaaa tttgattttc ggctcagttc ctgaaggacg gatagcgatc cagccatttt
    34801 cttccagcgt atacttcaat acgtcagatg gcggtgtcgt tagtttttct acgtttcctt
    34861 cacggtctgt tctcgtcaac tctttgaagt cttctgtttg gatgactttt gctccggcaa
    34921 attctgtcgg tgcttcttca cggaattttt ccatcaaagc agagattttt gcagagcctt
    34981 cttgtccgct cattgtcaca gaaatcgttt tttctgcgta gtagccatat gtttcaaaaa
    35041 tcgtttgcaa gccgtcatac aatgttttgc cttgtttttt atagtaagct gctacttctg
    35101 ctaataagag caatgcttga atcgcgtctt tgtcgcgaac gaaaggttta actaaataac
    35161 cgtaactttc ttcgaatccg aacataaaag tatgggaatg atcttcttca aattgttgaa
    35221 ttttttcagc aatgaatttg aatcctgtca atacattgaa catggtagcc ccatagtgtt
    35281 ccgcaattgc tgtaggcaat tcgctagaaa cgattgattt caataccgct gcattttcag
    35341 gtaaagtgcc tcgcgattgg tgtgcttcca aaatatattg agcaaacagg gctccgattt
    35401 ggtttcctgt caatacttca taggagccat ttggcaaacg aacggctgcg cctaaacggt
    35461 ctgcatcagg atccgttgca atcagtaagt cagcgccttc tttctcgcca agtcgcatcg
    35521 catactcgaa agcagagtgt tcttctggat tcggtgattt gactgtagtg aaatccgggt
    35581 ctgcgacagc ttgttctgga actagtacga attgttcgaa tccagcttgt ttcaacgcac
    35641 gttcgcccaa catttttcct gttccgtgaa gtggtgtgta cacaagcttc aaatctttac
    35701 ccattgtttc caccaattca tgatcaatcg ttactgcttt gatttcttct agataagcgg
    35761 catccacttc ttctccgatg atcgtgatca atccgctatg ttcagcttcg tcgtcagata
    35821 atacttcaac ttttaacgga ttgctgactt cacggacata tttcgtcaat gcatctgcgt
    35881 ctgcaggagg catttgtccc ccatcttctc cgtagacttt atatccgttg taagctgctg
    35941 gattgtggga tgcagttacc atgattcccg caaacgtctt caaatgacgg actgcaaaag
    36001 aaagctctgg tgttggacgt aaactttcga atacataaga agggatattg tgatgtgcta
    36061 atgttttggc tgcttccatg gcaaattctg gtgattgatg gcgtgagtca taagcgatag
    36121 cgacacctcg gcgttttgtt tcttcacctt gtgtatccat gaaacgtgca agtccttctg
    36181 ttgcttgtct taccgtatag atgttcattc gattgatccc aggtcctaag attccccgca
    36241 tgcctgcagt accgaattcc aacggtgcat aaaaagcatc ttcaagtttt tcaggatctt
    36301 cacccagatc atttaattgt ttttttaagt tttcttctaa attttcttca tgtacccatt
    36361 gttcatacac ttgttcccaa gacatgaaaa taacacctct ttctgaaata tggttcttaa
    36421 taagtatagc attcacttat tagatatgaa agtatgagga ccaaaaaacg cttaaatcaa
    36481 gaaaagattc ttttatcaag cacggttaca agtattcgca taaatcggat tcagtagatt
    36541 tcacgcaaaa aaaagagtaa aagaacaatt gaaaccgttc tttcactctt tttaaactta
    36601 tgcagttgtt tcttctaaag attccaagta tttatacact tgttgtccag caatcccacc
    36661 atcaccaaca gcagtagtga tttgtcgcaa atctttttca cgaacatctc cgatggcaaa
    36721 aacgcctggt atattcgtct tcatttcgtt gtctgttgga atccagccgg cttcgttcgt
    36781 caacccagct tctctaaatg gtgcagttaa tggatccaat cctacataga tgaatactcc
    36841 gtctgctgcc aaatcaaact cattgccagt tttgacatta cgtgctttca cacctgtcac
    36901 taccatttca tttccaacga tttcgtcaac gactgtatcc cacataaagg aaattttttc
    36961 attcgcaaat gcacggtctt ggatgatctt ttgtgcacgt aattcatctc tacggtggac
    37021 gatgactact tcagaagcaa attgtgtcaa atagatggct tcttctactg ctgagtctcc
    37081 tcctccgatc actactagac gtttgttacg gaaaaatgca ccatcacaaa ctgcacagta
    37141 agaaacgcca cgtccagcaa attcttcctc gccaggtacg cctaatttac ggtggataca
    37201 gcctgttgcg ataatgactg tttttccttc gtaagtttta tcttcacaaa taacttcttt
    37261 gtggctgccg tgatccttga tatcttgcac gattccataa gcattttctg tcccaaatct
    37321 tgaaacacct tcatacatct tataagctag atcaggaccc atgattgaat cgaaaccagg
    37381 atagttttcg atttctgccg tattgttcat ttggccgcca ggagcgcctt gttcgatcat
    37441 caatacggat aagtttgaac gtgaagcata taaagcagca gtcatcccag caggccctgc
    37501 gccaataacg attacatcat acatatgtag attctccttt acttaatcaa gtaagcatac
    37561 tttacagcca atgcgaaaaa aagtctattg ttctgcttgc gtttctcgaa tagctttatt
    37621 atactcgaaa aatccgctga aaaacaatag acttttataa atcattgatc aatcgttcga
    37681 aagattttct ccatttgaat caattacttt tttataccag ttgaatgatt tcttcttcga
    37741 acgggctaat gtgccatttc cttcgttgtc acgatcgaca taaatgaatc cgtacctttt
    37801 cttcatctca cctgtaccag ctgatactaa atcaatacat ccccaagtcg tatagcctaa
    37861 tagatcgact ccatctaatt ctactgcatc tttcattgcc tggatatgtg ctgccagata
    37921 gtcgattcga taatcatctt ccacataacc attctcatcg ggcgtatcga ctgcacctaa
    37981 gccgttttct acaataaata gtggtttttg ataacgatca taaatatcat tcatcgttat
    38041 tctcaagcct aatggatcaa tttgccaacc ccattcactt gcttccagat atggattttt
    38101 gacagaagca aagatatttc ctgcagtctg ttccaagagt tcgttgtcag tagtcgaaac
    38161 tcgagaagaa taataagaaa aggagataaa atcaacagta tgttctttca gcaatgcggc
    38221 atccccaggt tccattttga tctgaatgcc atttcgttcc atttctttca atgcataagc
    38281 gggatattct cctcgtgact ggacatcaat gaagaaataa ttttctcgat ctgactttct
    38341 agaagcccaa acatcttcag gattgcaagt gtatggataa tatgctcctg ctgccagcat
    38401 acagcctact ttgttctccg gatctacttc atgagcgatc ttcgttgcga tggcacttgc
    38461 aaccaactca tgatgtgccg cttgatattt gatttgttct tcattttctc cttcttcaaa
    38521 atacagccct gcgcccatga atggtgcatg aaggatcata ttgatctcat tgaatgtcaa
    38581 ccaatatttg acaagtcctt tatagcgatt gaagatgact cggcacagat tttcataaaa
    38641 accaacgagt tctctggaac gccacgcacc atattctttg atcaaatgca tcggacaatc
    38701 aaaatgtgta atcgtcacaa gcggctcgat cccgtacttt tgacattctt taaagacatc
    38761 ctcataaaat ttcaacccag attcatttgg ctctttttca tcgcccattg gaaaaatacg
    38821 actccaagca atggacatcc gataagtctt gaaccccatt tctgcaaata aagcaatatc
    38881 ttccttataa tgatgataca tatcgatcgc aatttttgct ggataaaaat gattttcatc
    38941 aaattcaaac attttctttt ttccagtaat gacaggaaaa cggtcttctc cgattggcac
    39001 aacatctacg ttcgctaacc cacgtccgcc ttcatcataa ccgccctcgc attgattggc
    39061 tgctgtagct ccgccccata aaaaatcttt tctgaatccc atcatacttc ctccattaca
    39121 tgtgtattgc ttgataattc tcttgccagt tcatcttgga cttcgattcc ttctgctttt
    39181 aaccggctga ttgtttcctt gaattgcggt aaatactgct gatgtgcaat aaacaactca
    39241 tccatgatcc gttttgctgt ctttcctgat cgtataagcg gattgatcgt aaacgcttgc
    39301 aaagctgttc cataatcacc agtcacggct gcttcgatcg tcagcaattc catattcttc
    39361 attacttgca accagcctct ttcagcagca ggaagggaac caaacgctac atttcgtgcg
    39421 ccttgtgcgc caacataggc agtgacttct actacacaat ctgccggcag atcaggaaca
    39481 gctccatcat tctttgtaga taccacgatc tgtgtttctt tatttccata gatcgaagca
    39541 atcgtttcac aggcagcatc tgaataataa gcaccacctc gtttttgcaa ttgttcaggt
    39601 ttatgatcca attctggatc tttgtataat tcaaacagct ctgcttctgt ttctttcact
    39661 tgctgcgctc ttgtaccaat cgtttgatat tcttctaaag catgttccag catttcttct
    39721 tcacgatagt aatagcggtg gtagccacaa gggatcatgt tcatttgttt taattgttct
    39781 ttgaaaaacg ggatgtcaaa gatatttttc ggcaatccat tgtcttcatc gtataaacga
    39841 tcaatcaatt ctgccgtgcg gtcatttcct ttattatctg ctactttgtg ccaatggaag
    39901 tgatttagac cggcaaactt gtagatcagc tcttcttttt tcttgccaag cattgttggt
    39961 tctgtcatca tcgccattac tggcacattg cataatccga tcactttatc ccatttgcca
    40021 tatctgataa ctgcttctgt caccatcccg cttgggttcg cgaaattaat cagccaagca
    40081 tttggacata aacgcttcat gtcttcaacg attgccaaaa ccactggaac ggtacgaaaa
    40141 gctttgaata tcccgccagc accatttgtt tcttgcccaa tcatcccata ataactaggg
    40201 atccgctcgt ctttgattcg tgcatctaat tgaccgacac gaaactgagt cgtaacaaaa
    40261 tccgcatctt tcaatgcttc ttcccgatcc agagttgcat agattttgac ttcatacgga
    40321 gaagcatccc acatacgctg agacatctga ctgacgatct caagcttttc ttttccttct
    40381 tctacatcta cgagccagat ttctcgtatc ggtaattcct catatctttt gataaatcct
    40441 tccatcaact cgggggtgta actgctgcct ccaccaatcg tagcgatttt gattcctttt
    40501 gccatgttct cactccttta ctttttacac ttttattatc ttttttgtaa acaaaacaaa
    40561 caatagaaat tcgtttcttt ccagtaacat ttacaaaagt tagtgttttc ttctggattt
    40621 ttgtaaatat cttgtcaaaa aatgtcttta ttaagctata taacataaat ccgtttacaa
    40681 atcattttca aaaaagttat aatttacgta aaaaaacaag aagaaggtat ctacatgtta
    40741 ttacgtgaaa aaatcaaaga aatctccttt tctccttcag aaaatgaagt gattcagttt
    40801 ctcttaagcg attctccagc acttggcgaa atgaccatcc aagagattgc tgagcgatgt
    40861 tatgtccacc cgtccacact catccgtgta gcaaaaaaaa tgggttttga tggctgggtc
    40921 agttttcgta atgctttttt agctgaaaca gcctatcttg aagggaattt tcaagacgta
    40981 gatgcgaatc ttcctttttc caccacagac ggtattatga cgatcgcaaa taaaatggct
    41041 tctttagaac agatgacgat tcaagataca ctttctctca ttcatcatga ccaactgcaa
    41101 aaagccaaac aactaatgct tcaagcaaaa aaaatcgtga ttttcgccag taactcgaat
    41161 actttgattt ctcaagattt catgctgaaa atgaagcgca tcaaaatgga tgtcacgatt
    41221 gtcaccactt ttggtgaatc gttctatgaa gcgtacaatt gcacaagtga tatgtgtggg
    41281 attctgattt cttatacggg tgaaaacaat gtgatccttc gtaccgcaga aatattgaaa
    41341 caaaatcatg tgccaatttt agctttgacc agtatcggtg attcaacgct atcttcaatg
    41401 agtacagccg ctttgcgact tacgacacgc gaacgactgt attcgaaaat tggtaatttt
    41461 acgatcaata catcgatttg ttatcttcta gacgtgttat atagttgtgt ttttgcagaa
    41521 aattatcaac agaatttagt ccatctaatt tctgtaggac aaaaatttga taaacgccct
    41581 atcagctcag ccatcatgaa agagcctttc aattttgaaa ataaagaagt ggactaaaaa
    41641 gaatagttaa gcctagtcca ttctttcacc taaccgttca ggtcagtgcg gtgataagtg
    41701 catcgtctgg cccgaccgtt tccccttgat tttccaaaat atccaaataa tccatgtaat
    41761 tgatgacaat cacaggtgtt tctaaactgt atccttcctc ttcgattttc tttttgtcga
    41821 agcgaagcaa cagatcgcct tgtttcactc tttctccttg ctgcaccaag ctttcaaaat
    41881 atttcccttc caactgaaca gtatccaatc cgacatgaat cagcaattcc agacctgctt
    41941 cagaaaccat cccgattgca tgtttcgtcg gaaacaggga catgactgtt ccgtcaaaag
    42001 gggcacgtac ttctcctttt tcagggtaga tcaagattcc tcgacctagt gttccttgtg
    42061 caaaagctgc atcctttgct gcattcagtg gagcgatttg tccttgtatc ggtgttttta
    42121 caatttcttt acctgatctt ttctgtctat catctgtttc atctgcttcc tccttccaaa
    42181 agaaaaaagt cagcgcaaag gataccacca tcgcaatggc gatcccaatc aatacattaa
    42241 tcacatctgt taaatcacca tctggtctaa tatagttggg aattgtaaag atgcctagtc
    42301 caccttgatt ccaagaagtt aaattcatta acccactata cgcgccccct aacgcaccac
    42361 cgacacaggt aaaaataaat atttttttac gtggcaatgt aaacccataa atagctggct
    42421 ctgtaatccc cacaatacca gaaacaatgg ctggtattgc aattgctttg tcttttggat
    42481 ttctaagctt aaagtagagt gcggtaagtg ctgcagtttg tccaaaagac actactgcaa
    42541 caggtgtcat aatattttgc caaccattca cagaaaattg catcaatccc attggtacga
    42601 ttgcccaatg gagaccaaac atcaccagaa tttgccagaa gaagcctacg attgctccat
    42661 aaataactgg cgaaagtgct aaaagtgttt caaatccagc cattaaaata tcagttgcga
    42721 atgtgaatac tggtccaacg agtaaaaaac ctaaaggcat tgtaataaga acggtgaaga
    42781 atggaacgaa gaaattcgcg atcatctcag gtactaactt tttaattggt ttctgaattt
    42841 ggctagctaa caacacaata aagataatgg gaatgacaga agaagcgtag ttacgcgcaa
    42901 tccagggaat gccgaatact gtttgataaa taggtgcttc aaaaatagtg ccagaaaata
    42961 tggtcgttag tggtgcttct gctgcaccac tcaaagcttc cagcgacaag ctaggattca
    43021 ttaatgctgc gccaagcaac atccctacga attcatttac tttaaattta cgggcagctg
    43081 ttaaaccgat aataacaggc ataaattgga atacagaatc accgatatta tcaaacataa
    43141 caaatgtgcc agaaccgcgg tcaaacgcgc ctaagaagac aaggaatgct gtcagtccac
    43201 gaatcatccc gtcagcagct aggatagcaa ggaatggttg gaaacagccc gatattccat
    43261 caataagcat atcaaacggt ctcatttttt ctgttgaaac agcctcatca aaagtaaggt
    43321 ttcctgcttt tataacctct tcaaacacga ctggcacatg attcccgatg acaacttgat
    43381 actgccctcc cgctcgcatt accgttacaa taccgtccat atttttcaag atctcatcat
    43441 ttgctttttc ttcgtttttt aatttgaaac gaagccttgt gatacaatgt gtcaaaccat
    43501 tgatattttc cttcccgccg acattttcta cgatttttct cgctaattct tggtattttc
    43561 ccatttctat tccctccaat ttattagtgg aaggtttagc caactgaatg tcactaccct
    43621 tatttcatac gttcttgtta atttatcctc ctttttttat ttatttacaa cgattctcat
    43681 gcactaaacg cgcagtatgg atcgttaaat acattttttc gtccgtggac atctggtagt
    43741 tgaacgtctc ggctaaaaag tctgctattt tctcaacgca accaaacgta tccttatatt
    43801 ttttttgaat aacctgaaac aattcctcag aaacttctcc cacttctgat ttttctccat
    43861 ctaggacgcg tgtcagaaaa aaacgtaagt gtgtgacgaa tcgaaaaaaa tggactgaat
    43921 cttcatcaaa tgtaatctta tagtaatagc ggacgatatt tagaatatcc tgtattgtta
    43981 tagtgaacag gtaggtattg tcttgttggc tttgaatgcc ttgtgcattg actaaatgca
    44041 atgcgataaa tccggcttca tcttctgata acttgatgtg gtatttatcc gagattttct
    44101 tcactgtttt ttttcccgat ggcatactct ttaggaaata gttgtttgat atcatacagc
    44161 aatacatttg gtacaaatat tcccattttg tgacgttcga tagccgtata caaatgatcc
    44221 gtcaatgcaa tatacacaga atcattcaac tggacttgta gagcctcttt tgtatattca
    44281 atgatttcac aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac
    44341 tgttgtgaaa gcccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaaaaatc
    44401 gggtcaccca ctttcttttt gaatgccaaa ccttttccca tcacgatcat ttcctgattt
    44461 ctttcatccc tggttattac gacgttgtta ttcagtactt tctcaatcag catgttttgc
    44521 tcctttccat gatatatagg gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc
    44581 atggaaccat gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt
    44641 atcgttttct ttatattatc gaaagcaaac gcttttgtca acgctttctt agtggttatt
    44701 agccgctttt gactagctgt cgtcgtcttt tttaaatcgg gtgactacgc cactaacgct
    44761 cacagatgaa gaatctgcga agctctgttt tattttcact ctagcgctta gggcaaaatg
    44821 ctactatgct actgtcacaa cctcttttta cacaccactg ctttgcagtt tgtacatatc
    44881 tgcatacaaa ccattttgcg ctagaagtgt ttcatgattc ccacgctcta ctatatgacc
    44941 tttgtctaaa actaaaatca attctgcatc ccgaatcgtg gacagccgat gggcaatcgc
    45001 tatcgttgtc cgtccttgtc gcatttttgc caatccttct tggatcaatc cttcggtttc
    45061 agtatcgata tttgctgttg cttcatccaa tactagtatt ttaggatctg tcacgattgt
    45121 tcgcgcaaaa gtgatcagct gtcgttgtcc actagaatag ctggcaccgt gttcgatcac
    45181 ttttgagtga tatccttttg gtaattcatc gatgaacttg tttgcttgaa caaactcagc
    45241 tgcatggcga atctgttcgt ctgtgatagc tggattcatc atccggatat ttcctgcaat
    45301 atctccataa aacaagaagg catcttgcaa gaccaatccc attttcttac gtaactcttc
    45361 cattggatat tctcgaatgt catgatcatc aatcaagatt tggccttcat aaaattcgta
    45421 aaaccgcatc aatacgttga tgatcgagct ttttccgctt ccggtgtgcc ccactaatgc
    45481 aacggtctca cccggattag cgacaaaaga gatattgttc aatacattgt gctctccatc
    45541 ataagaaaag cttacattgc gaaactcgat cttacctgaa gtaatcgctt gatctgctcc
    45601 aggattttgt tgcggtgcat attcttctgt atccatgatt ttcaaaatac gactaccggc
    45661 aaccatccca tctgtaaagg tgctcaaaaa gtccatcatt tgagacattg gattaaaaaa
    45721 ttcctggaca taggtcgtaa atgcatagat cagtccaact tctacgggag aatgcaacgc
    45781 atcgatccca aacaaagtca acgccaacgc aatcgccaat gcatagagaa aattgatgat
    45841 tggacttaac agaatcgaat tcgttttgat catcgcatag cgcgtgtcta aatactcgtt
    45901 gttcacttgt tcaaattctt tagctaaacg agcttcttgg cgaaactgct ggataatacg
    45961 catgccggaa atcgattcat tcaatttcgt gttcagctga cttaattttt cccgcatatt
    46021 tcggtaaata cgagaactga atttctggta ataccagatg acgattccta aaatcggcaa
    46081 gaacaccaaa gtccacaagg caatttctcc attgatttga aacatggcaa cgaaagaaga
    46141 aacgactgca aagatcccag tcaataccat caaaaagaca taccaaaatt caaatagcgt
    46201 ttctgtgtca ttcgtcactc gagaaacggt cgatcctgcc ggtgtctggt caaaataacg
    46261 catacctagt gtatgcagct tttcaaataa tcttacccga acatactgat aggtcttcaa
    46321 tgaagccatg gaatacaaat accattggaa aaaccaaatg atacttttga ctatgactcc
    46381 aaacaagtac acaccagcaa aaaacaaaat aacttgtgtc gtagctgact gtttagccag
    46441 atgatcatcc ataaaagttt ggatgatcct tggcaatagg acattgatca cagacagggc
    46501 aaacgcaaat aagatagcaa cgatgaacgt tcttctaaaa ggtttggcga acttcatcag
    46561 tcgtttgatg atcttcttct gttctttaag tggaatcgat tttgtccatt ccgattgata
    46621 attttcatcc atcaggcctc gcctccttct atcttcgctt ctaattgctg tttttcccac
    46681 attcgttcat accagccacc taatgcaagc agctcgtggt gggttcctcg ttcaatgatt
    46741 ttcccttgat cgacaacaat gatttctttt gcatgcatca cgctgcttaa acggtgagct
    46801 gcaatgatgg ttgttttctc ttttcttatt tcttttaaat tatgcaagat agcttcttct
    46861 gttttggcat ctactgcaga aagtgcatca tctaagatca gcaattcagg atttttgatc
    46921 aatgctcgag caatcgaaat cctctgtttc tgtccgccag ataaagagac tccacgttct
    46981 ccaactaagg tatcataacc ttttggcaat tccttgattt cattgttgat aaatgctaaa
    47041 gaagccgcat gctctacttc ttcttctgaa agttttggat tagaaaaacg gatattatct
    47101 cgaatggtca tcgaaaacag gaagtgatcc tgaggcacat aaccgattga tcctaataaa
    47161 gcattcaatg aatattcttt gatcgaacgt ccgccaaatt ggattttccc ttgataatca
    47221 tcgtattcac gcatcagtaa tttgatgatt gtcgtttttc ccgcaccagt tttcccaaca
    47281 atacctaatg tttctccttc ttggatcgca aatttgattt gttctaatgc tggtgattct
    47341 tctttcggat agttaaaaga ggaaatagct acattcagct cgccttttgc cggggtctgc
    47401 actgcaccca gttgttccac gatatgcgtc ttttcatgca aaagttcatt gacgcgatca
    47461 taagaagcat ttcctctttc caatacgttg aacaatcgac caatcgcaaa catcggccaa
    47521 acaagcatac cgatatagct aataaatgag accatctgcc cgattgagat cgtgccttcc
    47581 aagacatact ttccacctaa aataattgtt gcaacataag acagaccgat aatcaaggta
    47641 atgaatggat caaataagga gtctaaaaaa ttgactcgtt tgtttttaat aattgcatct
    47701 tcaatctttt cttcaaaatc cgcgagatct tctttttctt gtccaaatgt tttaatgact
    47761 ttgatcccag tgatactttc ttgtgtctta tcattgatac tagaaaaagc ttcttgcgaa
    47821 tcacgaaaag catcgtggag ttttgacccc agcactcgtg aagtaacggc tagcaaaggc
    47881 agaggaatca gcgcaatcag tgttaagcgc caatcaataa acaaaatcat cgcaatgatc
    47941 gttgttccgc ccgtaatcac tgaatctgca aaggtcaaga ttccagctcc tgctacattc
    48001 tggattgcat tcaaatcatt cgttgcatga gccatcaaat ctccagtccg atatttttgg
    48061 taaaagagat tgtccatttc tgtaaaatgt ttgaatagct gctgccgaag ttctctctcc
    48121 aaccgagctg cacttcccca aatattcgtc cgccagatat aacgaaaaag atattgtaag
    48181 aaagctgcaa ggatcaataa taagatccac caggaaatga tctttattga taaattctta
    48241 tcggcgatct catcaatgat gatcccaatc acttttggag gaactaactg taccaaggcc
    48301 actgcgaata gggagaaaat cccaattaaa taatgtttct tttcctgacg aaaaaaccaa
    48361 cctaattttc taaaaatcga cattttactc ctcctttgtc tttatcaaga gcataggatt
    48421 gtctgtgctt gtcttttttg ctgaatagtc tgaatagtct aacaaattac gaaataaaaa
    48481 acggactgaa aaaatcagcc cgcaaaaagt ctttgggtca gaacctattt tttcccttgg
    48541 ttcttcattt gctgcatcat ctggcgaatt ttcttctcag atggtttttg ccccattgac
    48601 atcatcattg atcgcaacat gtcctcatta acgggaggat ttttttctaa atagtctttc
    48661 atatatttac gcgctaggaa aaagccacct actgcgccga ttagcaaagc aataatagca
    48721 ataagtacga caatacctgt tgataccata taaatctctc ctttctattt ccgaattgct
    48781 ctcaatgatt tattttacta aagtttattg aaaaaagaaa ggcgtattct ttagtttcat
    48841 gcaaaaaata gtggtttttt tagagaagaa caaaatttct ttcattaacc agcaagacac
    48901 accacgaaaa aaaattaggc ataccgaatc tttttcttta attttttctt tctgaggtgt
    48961 agaatgtaat cgtagataaa taggaaaggt ggttttttct tgaataatca atccaacaaa
    49021 caatttcata agcatcgact tattgaacat acaaatggtt tatctttagc tgaagtaaat
    49081 ggaacagtgg atgtaccaaa gggaaaaagt ttttttaaaa cacttcttgc ttactctgga
    49141 ccaggtgcat tagtagcagt tggttatatg gatccaggta actggtccac ttcgatcact
    49201 ggtggacaaa atttccaata tttattgatg tcagttattt tgatgtctag tctaattgct
    49261 atgcttttac agtatatggc agcaaaatta ggaatcgtga cccaaatgga tttggcccaa
    49321 gcaatccgag cacggacaag taagacattg ggtgtcattt tatggatctt gactgaatta
    49381 gccattatgg caacagacat cgcagaagtc attggtgcag caatcgccct ctacttactt
    49441 tttgatattc cattggtgat tgccgtcttt attactgtat tagatgtttt cgttctctta
    49501 ctactgacaa aagtcgggtt tcgtaagatc gaagcactgg ttgtctgctt aattttcgtc
    49561 attttattcg tttttgtcta tcaagttggc ttgtctaacc cagattggca cagtttattt
    49621 gctggcttta tccctaacag tcatacattc tcagactcac cggtcatcgg cggcgaaact
    49681 ccgctgactg gagctttagg gattattggt gcaacagtca tgccgcataa cttatatctt
    49741 cattcagcag tttcacaaac tcggaagatc gatcaccaaa acgaagaaga tgtagcacaa
    49801 gctgtacgtt tttctacttg ggattctaat atccagttgt caatggcttt tctagtcaat
    49861 gcactgctat tgatcatggg tgtcgctgtc ttcaaaaccg gttctgtcca agatccttcc
    49921 ttttttggtc tgtatgaagc tttatctgat ccatcagcta tgagcaatgg tattttgatt
    49981 gctgtagcaa aatctggtat cttgtccact ttatttgctg tagcacttct tgcttctggc
    50041 cagaactcta ctattactgg tacgttgacc ggtcaagtca tcatggaagg attcatccat
    50101 atgcgtatcc cgatttggct aagaagactg gtcacacgat tgctttccgt gattcctgta
    50161 ttgatttgtg ttttgtttac aagtacaaaa ggaacgatcg aagaacatgc cgctttgaat
    50221 aacttaatga atgaatccca ggtgttcttg gcttttgcgt tgccattttc gatgattcct
    50281 ttactgatga tgaccaacag ccaagcggag atgggtagcc gatttaaaaa ttcattcatc
    50341 gtaaaacttc ttggctggtt ctctgtgatt agtctaactt acttgaactt acgaggatta
    50401 ccaggacaga tcgaaagttt cttcggcgag caagccactt ccgctcaact agcattggct
    50461 gatcacatcg cttatgtaat cattgttctt gtccttctcc tgttggcatg gacgattggt
    50521 gatttatata aaggaaacaa aaaatatgaa cagcttttgc aggaaacggt agaagaaatc
    50581 gaataaaaac tgatttttcc acacaaagac ccttgatagt taggttaaaa atctagctac
    50641 caagggtctt tttatttaga agataaagct tttttcagat ttaatttact gaaaactagc
    50701 atgcttgtta ctacgatgaa ttgcgttaat ggcacgcctg caccagtgta gacacttaaa
    50761 aaatagtcct ttttttctgg cgcataagaa caaaaccatg agacaccgaa aagaaaaacc
    50821 aattgtgtca gtattaatcc ggtgaccaca atcaaagagg aactataata ggcacgatca
    50881 gcttttgcaa atacggttcc tagtatcata agtaatatcc ctgctattga agcaatcagc
    50941 acgggaatag ccagaacttg gtaaattttt atgatgacat tcgcgacttt tgctgatttt
    51001 gttaattgga aattaggtgt tctcggttca ttactatcga tccaattctc cattagcgga
    51061 aggtttaata attcttgagc ctgtttgact tgttcttgcg ttccgaaact ataatttcct
    51121 ccttgatgga aatctttata tcgccatgca taagcgtatc ctgattgaag gaaatcgaat
    51181 acgattggca gatcactcat cgtctttcca tctcctgatc ccatcagata gaactctttt
    51241 ttctttttga tggttccctt gtgataggct tcttctaatt cttggttgac ttttttccaa
    51301 aaatcattgg tttcttttgc attgttttgg taataaccag attcgcttac cacatctcgc
    51361 aatgcccaaa agatgatatc tccgggtaat tcttttactt ttccaccttg agtccacgcg
    51421 tgagtcgtat ataattcgtc aatacgggta gaaacagtat ggaatgtagg agatgcggct
    51481 aatgctttat ttagtgcagc atgtgagacc catatctcac tgttttcttt attcaagttc
    51541 gtttcatcat ctattcggat cagttgtttc atcaaacgtc caaattctcc actggaacgg
    51601 tcattgatgg ttgcaatacc gtatgttgtc tcatttttat attcgatcca ctgatagacg
    51661 atggaaaagc caactatagg aagtaaaaac aatgaaagtt tcagccattc ccgtttcgag
    51721 agaagaaaga gtcgtttttt taacttcttt acttctttga aagaaaagtc ttgccctagc
    51781 aagatctgga cgaaactgat acccaaagct actacagtaa aaggaagaag ccagatagaa
    51841 tcttcgcgaa tgaaccaaaa gaaaggaaaa gcgacagaaa gccctaaaaa ccatggcagc
    51901 aattttctgc tagaaccgta tttgcggtaa taaatagcta ataaacaagc gataatgatc
    51961 tcaacagccg gaatcacgag ggcattacga tatgttcgag tggaatattc atgtgtaaaa
    52021 ctaatcggag aaaacaaaaa ataaaaatag atacttaaaa gaagatactt gttcttcaca
    52081 atcggtttaa acgcgagtgc aaccaatata ctggcaaaaa tactgaacat gccgaacaaa
    52141 actgcataag aaagatgcag tttatgtgca agtactaaaa ataagctgta ggagattcct
    52201 ttggaaagtg ttgtagtcgt atactcccct agccatttac catccaaaat attttgagca
    52261 tagtgaatca gcagctgatc atcataggtc gccgacatat ctgttccata gattgcttta
    52321 ctatataaat acaaccgaat aattgtaaat aatacggcta tgccaagaat aaagtatgta
    52381 tgtttctttt ttagttggtc gatcaagggg tttaaaatgt tcagcatctt ttcgtccttt
    52441 caatcacgtt ttgctacttg tttgtgaaac ttttcgtttt ttacttttct atcagtatac
    52501 cctgtttgtc tgaatttaaa aagagggggc aaaaggagca tggcgaaaac gaatgcaaaa
    52561 agcactcctt tgtttcttaa acaaaaaagg acaaggcaga ctgcccaatc cttttttgtt
    52621 gctgtcaggt agaaaactta aagtgtgact tcttctgcta ataactcagg gaacacttcg
    52681 ggatcgaatg cttgcgcttt gaacatggaa atttcttttc catatggtgc gtaagctttc
    52741 tttttatctt taccgacata tggggtttct aatattttcg aaatgttgtc aaacattgga
    52801 tcatggacga ttttgttcaa tgtgtcaaat ccaattgttc caaaaccgat attggcatgc
    52861 cgatctttat gagaaccttg tggattttta gaatcattga tatgcagtac tttaagccga
    52921 tctaatccga tgaccttatc aaactcttcc attacacctg aaaaatcttc ttttatatta
    52981 tatccggcat cattcgtatg acatgtatca aaagtaacgg ataatttttc attcaatttt
    53041 actccatcga tgatttgcgc taattcctca aatgttcgac cgatttccgt acctttccct
    53101 gccatcgtct ccaaagcgat ctgtgccgtc tgttcttttg tcaaaacttc attcaacccc
    53161 tcaataattc tagcaatccc agcttctgcc cctgctccta catgagcgcc tgggtgaagg
    53221 gtgatttgag tagcacctaa agcttccgca cggacaatct cttctctcag aaattgtaca
    53281 gcaaatggaa acatttctgg tttgatcgta tttcccaaat taatgatata aggcgcatga
    53341 acgacgatat ttgttagtcc gttttctgcc ataaatgctt ttccggcatc aatattcatc
    53401 tcttcgatag cttttcttct agtattttga ggagcgcctg tatagatcat aaaggtcgaa
    53461 gatccgtaac tggctgcttc ttctgccgcg cctaacagca ttttctttcc gctcatgctg
    53521 acatgtgagc ctaataacat ggatggattc ctccttgaaa ggcaattgtt tcgaacaatt
    53581 cctgtatatt gataagatga tgtacaaaga aaatggtttg tttttctttg ccctgttctc
    53641 catagtaact gaaactttcc ctaaagaaaa gcttaagaac tctttcataa aaaagatcat
    53701 ttcctcacgt tttatctcat tcgaaaaaga aattcctgtc aaaaacagcc ttgtctgttt
    53761 ttttgtccta ttatttgcta taattagcga aataaaacca ttagttgagg tgaccctttt
    53821 gccacgcaaa aatactcgaa aaaaacagaa tagaagaaaa aaagaaaaat ggtttgttcc
    53881 taagattatt tttagagtgt ttcaatcatt gactgttttt attactgttc ttcttttgtt
    53941 gttcgcagct ctaggtatcg gaataggtgc tggctacttt gcctatcttg tcgaagacac
    54001 acaattgccg actaaaaaag aactgcagac agaactagga aacattacgg aaacgtcaaa
    54061 gattgtttat gcagataata cagagatatc aaaaatccag acagatctga tgcggacgac
    54121 tatttcatct gataagattt cgcctttatt gaaaaccgcg attatcagta cagaagatga
    54181 atacttcgat aaacatcaag gttatgtccc taaagctgtt ttacgagcac tagtttcaga
    54241 agcgactggc atcggctcgt ctggaggatc cactttgaca cagcaattag tcaaacaaca
    54301 gatcctaacc gatgaaacaa cattcaaacg taaagcaaac gagatcgttc tttctgcaca
    54361 agtagaaaaa cacttttcaa aaaatgagat cattactacg tatttgaatg tctcaccatt
    54421 tggtcggaat aataaggggc aaaatattgc tggagtccag gaagctgcta gaggaatctt
    54481 tggtgtagat gcaaaggatg tgactctgcc acaagctgct tatatcgctg gtttgcctca
    54541 aagccctatc acgtatagtc catacacgaa tacaggcgcg ctaaaagaag atctttctgc
    54601 cggtcttgcc agaaaagatt ttgttttatt cagtatgtac cgtgaaggac aaattacgaa
    54661 agaacaatac gaagaagcga aagcctatga tcttacaaaa gactttctcc cacaacaaat
    54721 cgcggaacag actgacagag aattcttgta ttataccgtg atgaacgaag caacaaggat
    54781 tattgcacaa caattggcag aaaaagataa tgctgatatg tcagattcat cggtcagtga
    54841 agcttattat caaaaagcac agcaaacgat ccaaaacaaa ggatatacga tccattcgac
    54901 gatcgataaa gatatctatg ctgcattaca aaccggcgta gaaaattatg gctacatgtt
    54961 agatgatggt gctggtagcc aagtggagac tggaaatgta ttgatggata acagaaccgg
    55021 acgtatctat ggcttcgtgg gtggtcggaa ttacagccag aatcaaaata accacgcctt
    55081 tgatacagaa cgtcaagctg gttcatccat caaaccggtt cttgtttacg gtcctgcaat
    55141 cgatatggga cttgtcggat cagaatcccg tgtatcagat tatgcaacga cttggcaaga
    55201 aggaaaaaat gctggtgaga aaatcgtcaa tgcgaccaat gaagggtcga acaccttcca
    55261 aaccgtccgt gagtcactgg aatggtccaa caatatccca gcttatcact tatatcaaga
    55321 tgtgttgaat tccggtggat cgaaacaata cgcttacgag cactacttgg cgaagatgaa
    55381 ctacccagca aatgataatt ggggcgttga atcggctcct ttagggacag tagatgtaac
    55441 gacgcttcaa cagacaaatg gattccaggc cttagcaaat ggtggagtct acgaagaagg
    55501 ctatatcatt gattccatca cagataatgc aggtaatgtg atttataaac atgaaagtaa
    55561 ttctgtccgt atttattccg aagcaacggc atcgatcatg aatgacatga tgcgtagtgt
    55621 gatcaatgaa aaaatcacta caccattcaa agatgtcatc tcaagcttaa acggaaatct
    55681 aggaaatgca gattgggtcg gaaaaactgg ttctaccaat gaataccgag attcatggct
    55741 gatcgtctca actccatcta tcactatcag tagttgggca ggacacgatg acaatactgg
    55801 aatggacagc caagcaagaa tacgttcggc taattatctt gccaatctaa tcaatcaagc
    55861 ttaccaagca aaccctgaga ttttcggcac tgatgagaaa tttgacttat catccgatgt
    55921 gatgaagaaa aaagtatctg ggttcacagg acaaacagct ggaaaagtta ctgtcaataa
    55981 aaaatccatc caaacgccag gaaaaacagt ggagtcactt tgggcaaaaa atggtccgaa
    56041 gaaaagcgcc tttaaattcg gcgtaggcgg tactgatgaa aactatacag attactggaa
    56101 caccgcttca gcttatgcta gagcgaatcc agagaccaaa gaatcagacg ataaagatga
    56161 ttgatctaat agattaatca gaattaaagg gagctgcgac aaaaatcttg tcacagctcc
    56221 ctttttccga ataaatggtg ttcaaaagct tacatctacg atattaagac aaatttatgt
    56281 aaaaatgtga aaaac
//
LOCUS       DACEJR010000009        52640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEJR010000009 DACEJR010000000
VERSION     DACEJR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.009"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..971
                     /locus_tag="IW651_000598"
     CDS             117..971
                     /locus_tag="IW651_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0813349.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="IW651_000599"
     CDS             972..2309
                     /locus_tag="IW651_000599"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0813350.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="IW651_000600"
     CDS             2314..2538
                     /locus_tag="IW651_000600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0813351.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="IW651_000601"
     CDS             2540..3430
                     /locus_tag="IW651_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0813352.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="IW651_000602"
     CDS             3439..4257
                     /locus_tag="IW651_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0813353.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="IW651_000603"
     CDS             4364..4813
                     /locus_tag="IW651_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0813354.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="IW651_000604"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="IW651_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0813355.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="IW651_000605"
     CDS             complement(6568..6684)
                     /locus_tag="IW651_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0813356.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="IW651_000606"
     CDS             complement(6757..7713)
                     /locus_tag="IW651_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0813357.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="IW651_000607"
     CDS             7884..8252
                     /locus_tag="IW651_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0813358.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="IW651_000608"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="IW651_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0813359.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="IW651_000609"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="IW651_000609"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0813360.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="IW651_000610"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="IW651_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0813361.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="IW651_000611"
     CDS             10240..12432
                     /locus_tag="IW651_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0813362.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="IW651_000612"
     CDS             12458..13420
                     /locus_tag="IW651_000612"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0813363.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="IW651_000613"
     CDS             13434..14795
                     /locus_tag="IW651_000613"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0813364.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="IW651_000614"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="IW651_000614"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0813365.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="IW651_000615"
     CDS             15924..17144
                     /locus_tag="IW651_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0813366.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="IW651_000616"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="IW651_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0813367.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW651_000617"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW651_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0813368.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="IW651_000618"
     CDS             19906..20583
                     /locus_tag="IW651_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0813369.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="IW651_000619"
     CDS             20597..21196
                     /locus_tag="IW651_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0813370.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="IW651_000620"
     CDS             21211..21498
                     /locus_tag="IW651_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0813371.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="IW651_000621"
     CDS             21547..22329
                     /locus_tag="IW651_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0813372.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="IW651_000622"
     CDS             22350..23057
                     /locus_tag="IW651_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0813373.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="IW651_000623"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="IW651_000623"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0813374.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="IW651_000624"
     CDS             complement(26166..27692)
                     /locus_tag="IW651_000624"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0813375.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="IW651_000625"
     CDS             complement(27707..28354)
                     /locus_tag="IW651_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0813376.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="IW651_000626"
     CDS             complement(28538..28867)
                     /locus_tag="IW651_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0813377.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="IW651_000627"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="IW651_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0813378.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="IW651_000628"
     CDS             29788..30801
                     /locus_tag="IW651_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0813379.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="IW651_000629"
     CDS             30801..32078
                     /locus_tag="IW651_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0813380.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="IW651_000630"
     CDS             32141..34843
                     /locus_tag="IW651_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0813381.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="IW651_000631"
     CDS             34995..35312
                     /locus_tag="IW651_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0813382.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="IW651_000632"
     CDS             35342..35662
                     /locus_tag="IW651_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0813383.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="IW651_000633"
     CDS             35748..37208
                     /locus_tag="IW651_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0813384.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="IW651_000634"
     CDS             37276..37497
                     /locus_tag="IW651_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813385.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="IW651_000635"
     CDS             37528..37710
                     /locus_tag="IW651_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0813386.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="IW651_000636"
     CDS             37710..38123
                     /locus_tag="IW651_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0813387.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="IW651_000637"
     CDS             38246..39427
                     /locus_tag="IW651_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813388.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39816..39889
                     /locus_tag="IW651_000638"
     tRNA            39816..39889
                     /locus_tag="IW651_000638"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
     gene            complement(39958..41097)
                     /locus_tag="IW651_000639"
     CDS             complement(39958..41097)
                     /locus_tag="IW651_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0813389.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41396..42031)
                     /locus_tag="IW651_000640"
     CDS             complement(41396..42031)
                     /locus_tag="IW651_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813390.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42144..42779)
                     /locus_tag="IW651_000641"
     CDS             complement(42144..42779)
                     /locus_tag="IW651_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ0813391.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42813..43274)
                     /locus_tag="IW651_000642"
     CDS             complement(42813..43274)
                     /locus_tag="IW651_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ0813392.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43404..43835)
                     /locus_tag="IW651_000643"
     CDS             complement(43404..43835)
                     /locus_tag="IW651_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0813393.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(43853..44173)
                     /locus_tag="IW651_000644"
     CDS             complement(43853..44173)
                     /locus_tag="IW651_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0813394.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44472..45248
                     /locus_tag="IW651_000645"
     CDS             44472..45248
                     /locus_tag="IW651_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0813395.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45263..45466
                     /locus_tag="IW651_000646"
     CDS             45263..45466
                     /locus_tag="IW651_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813396.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45482..45820
                     /locus_tag="IW651_000647"
     CDS             45482..45820
                     /locus_tag="IW651_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0813397.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            45807..45986
                     /locus_tag="IW651_000648"
     CDS             45807..45986
                     /locus_tag="IW651_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813398.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46029..46499)
                     /locus_tag="IW651_000649"
     CDS             complement(46029..46499)
                     /locus_tag="IW651_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813399.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46586..47284
                     /locus_tag="IW651_000650"
     CDS             46586..47284
                     /locus_tag="IW651_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0813400.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47462..47803
                     /locus_tag="IW651_000651"
     CDS             47462..47803
                     /locus_tag="IW651_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813401.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            47796..48467
                     /locus_tag="IW651_000652"
     CDS             47796..48467
                     /locus_tag="IW651_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0813402.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48473..49159
                     /locus_tag="IW651_000653"
     CDS             48473..49159
                     /locus_tag="IW651_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813403.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            49162..49911
                     /locus_tag="IW651_000654"
     CDS             49162..49911
                     /locus_tag="IW651_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813404.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            49923..50192
                     /locus_tag="IW651_000655"
     CDS             49923..50192
                     /locus_tag="IW651_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813405.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            50354..50656
                     /locus_tag="IW651_000656"
     CDS             50354..50656
                     /locus_tag="IW651_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813406.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            50653..50814
                     /locus_tag="IW651_000657"
     CDS             50653..50814
                     /locus_tag="IW651_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0813407.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            50811..51116
                     /locus_tag="IW651_000658"
     CDS             50811..51116
                     /locus_tag="IW651_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813408.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            51116..51472
                     /locus_tag="IW651_000659"
     CDS             51116..51472
                     /locus_tag="IW651_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0813409.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            51432..51677
                     /locus_tag="IW651_000660"
     CDS             51432..51677
                     /locus_tag="IW651_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813410.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            51674..52093
                     /locus_tag="IW651_000661"
     CDS             51674..52093
                     /locus_tag="IW651_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0813411.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            52090..>52640
                     /locus_tag="IW651_000662"
     CDS             52090..>52640
                     /locus_tag="IW651_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0813412.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
BASE COUNT        17789 a         8801 c        10970 g        15080 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    39901 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    39961 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    40021 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    40081 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    40141 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    40201 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    40261 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    40321 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    40381 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    40441 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    40501 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    40561 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    40621 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    40681 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    40741 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    40801 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    40861 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    40921 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    40981 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    41041 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    41101 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    41161 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    41221 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    41281 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    41341 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    41401 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    41461 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    41521 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    41581 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    41641 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    41701 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    41761 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    41821 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    41881 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    41941 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    42001 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    42061 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    42121 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    42181 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    42241 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    42301 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    42361 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    42421 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    42481 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    42541 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    42601 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    42661 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    42721 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    42781 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    42841 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    42901 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    42961 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    43021 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    43081 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    43141 aaggggatag ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta
    43201 ataattgaaa tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa
    43261 atctcttttt ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca
    43321 tatatgagga aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt
    43381 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    43441 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    43501 tccataaatt tttcgagatt aaactgagat ttatcagtca gttcgttctc aatatagata
    43561 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    43621 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    43681 tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt
    43741 ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg
    43801 tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat
    43861 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    43921 ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc
    43981 tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta
    44041 attcatctga attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa
    44101 aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt
    44161 cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat
    44221 acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg taaacagatt
    44281 taacaactaa aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag
    44341 ctttaaaatc aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca
    44401 gatactgtca attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag
    44461 gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa
    44521 ttttagtaaa tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt
    44581 caaacactaa agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa
    44641 tcacgacctc aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt
    44701 taatcttaaa atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag
    44761 tgttgccagc aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt
    44821 tgcttaatcc agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa
    44881 gattaatcgc agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca
    44941 tattatcttc tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc
    45001 cacaacagat gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat
    45061 ggttattgtg caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc
    45121 cgaaagccga tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc
    45181 tatttatcta cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa
    45241 ttggttagaa aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga
    45301 aaaaatcaaa gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag
    45361 atttggaatt agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga
    45421 agcaaacaga attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct
    45481 aatgcaatat ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt
    45541 ggattatgag gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt
    45601 gctagaacgg attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag
    45661 atatagaaat attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga
    45721 aggaagagac acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt
    45781 ttcggaaatc ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg
    45841 caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga
    45901 ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga
    45961 aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt
    46021 actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata
    46081 tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta
    46141 aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt
    46201 ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct
    46261 cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat
    46321 gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat gattactttt
    46381 gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc
    46441 attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc
    46501 cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat
    46561 tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
    46621 tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag
    46681 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa
    46741 tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt
    46801 cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga
    46861 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
    46921 tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa
    46981 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
    47041 agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
    47101 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
    47161 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
    47221 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga
    47281 atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata
    47341 aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
    47401 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
    47461 tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc
    47521 gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact
    47581 agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat
    47641 ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat
    47701 tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg
    47761 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga
    47821 actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg
    47881 attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg
    47941 taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg
    48001 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
    48061 cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag
    48121 ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat
    48181 tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta
    48241 gaatcgatac aaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga
    48301 tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag
    48361 ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga
    48421 tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa
    48481 accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataagcgc
    48541 aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg
    48601 tgatcgagtc attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa
    48661 cttcatctat gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg
    48721 gaaggaattc ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga
    48781 cgaatcgaat acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat
    48841 ctttgaacat catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata
    48901 ttacttctac aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga
    48961 catcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga
    49021 gtacacattt gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat
    49081 taatttcaaa aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa
    49141 gttaaatatt ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc
    49201 aacgtaaggt acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg
    49261 gccttgtgta atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat
    49321 ggtgtgaata aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg
    49381 aatcgggaaa tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt
    49441 aatcaatacc ctattgacga aaaacgcaat acccctattg acgaaaaagt caaagataat
    49501 aatacatcta ttaataatac atttaataat acaaaagaat atataagaga gttaccgcct
    49561 tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt
    49621 ttgtcagatg agcagatgga gaaactcaaa acagaattcc ctaatgatta ccaagagcga
    49681 atcgaacgac tgtcagagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca
    49741 actattcgaa gttgggcaag gaaagaaaaa agtgaaccta agaacgcaag cagtggatac
    49801 aagcgcacag gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga agcctatctc
    49861 aagaaaaaag cgctagaacg agctaataga caatcaaaag caccattcta agaggtggaa
    49921 aaatgaagat cgattatcta gaactaatta atgaaatagc gaattataaa aagggcgagg
    49981 aattagacgt cctgagagac gtatatgatc aactcgaaga agctggaatt gaaggaatta
    50041 agaatgatcg ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa
    50101 cacaattaag aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc
    50161 aaaaacatct taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca
    50221 cagattgaac taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg
    50281 ctcaacacgt tccatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa
    50341 ggagaatgtc gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc
    50401 agaagattgg caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat
    50461 gtgggcagac aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa
    50521 acttctagtg atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat
    50581 cgtgaggaaa agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt
    50641 ggaggaatca tcatgacaac agaagaagtg attcaaatgc gtattcgaag ccttcagcgt
    50701 gagattgacg atctggaacg aacaaaggca gtgatggtca atgaaacggc gagaaaggca
    50761 atcgatttgc acatagagaa tttaagaagg gaaatccatc gattggagga atgagcgtgg
    50821 ataagaaagc agcaatgaaa agaattgctg aattaaccaa gtcagaatct tggcaagaag
    50881 acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca
    50941 aacggagaac gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta
    51001 ctgctgaaca gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta
    51061 ggagcttatg gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga
    51121 tctcattaca caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga
    51181 taaaaaatca aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg
    51241 agtgcgaagt agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat
    51301 tcaaaaaggg ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact
    51361 tcatcaagat cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag
    51421 gaggacagca aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat
    51481 caggagcaac actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc
    51541 agattatttt gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag
    51601 aatcaaaagc ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg
    51661 aggaggacag cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag
    51721 agatgtagcc gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat
    51781 aaatcctacc gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg
    51841 aatgaaagat aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg
    51901 aaatggcttc gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc
    51961 cggattagtt tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct
    52021 gtttgaagaa tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga
    52081 ggaacagcga tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga
    52141 aatgatgcaa tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa
    52201 aaaatagacg aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa
    52261 tctacggacc cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt
    52321 gattcatatt atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga
    52381 gaagaattac tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac
    52441 ggctacgagg ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt
    52501 actaaatgtg ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct
    52561 catcgattgc ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga
    52621 acagtggaag aggtggtaga
//
LOCUS       DACEJR010000010        46944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEJR010000010 DACEJR010000000
VERSION     DACEJR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.010"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(200..1591)
                     /locus_tag="IW651_000663"
     CDS             complement(200..1591)
                     /locus_tag="IW651_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0813413.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1804..2190)
                     /locus_tag="IW651_000664"
     CDS             complement(1804..2190)
                     /locus_tag="IW651_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0813414.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2246..2869)
                     /locus_tag="IW651_000665"
     CDS             complement(2246..2869)
                     /locus_tag="IW651_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0813415.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2933..3601)
                     /locus_tag="IW651_000666"
     CDS             complement(2933..3601)
                     /locus_tag="IW651_000666"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0813416.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3613..4053)
                     /locus_tag="IW651_000667"
     CDS             complement(3613..4053)
                     /locus_tag="IW651_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813417.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4112..4783)
                     /locus_tag="IW651_000668"
     CDS             complement(4112..4783)
                     /locus_tag="IW651_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0813418.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4968..5366)
                     /locus_tag="IW651_000669"
     CDS             complement(4968..5366)
                     /locus_tag="IW651_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0813419.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5363..6136)
                     /locus_tag="IW651_000670"
     CDS             complement(5363..6136)
                     /locus_tag="IW651_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0813420.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6133..6816)
                     /locus_tag="IW651_000671"
     CDS             complement(6133..6816)
                     /locus_tag="IW651_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0813421.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6983..7870)
                     /locus_tag="IW651_000672"
     CDS             complement(6983..7870)
                     /locus_tag="IW651_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0813422.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7867..8736)
                     /locus_tag="IW651_000673"
     CDS             complement(7867..8736)
                     /locus_tag="IW651_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813423.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8873..10159)
                     /locus_tag="IW651_000674"
     CDS             complement(8873..10159)
                     /locus_tag="IW651_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0813424.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10432..11010
                     /locus_tag="IW651_000675"
     CDS             10432..11010
                     /locus_tag="IW651_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0813425.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11010..11939
                     /locus_tag="IW651_000676"
     CDS             11010..11939
                     /locus_tag="IW651_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0813426.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11936..13672
                     /locus_tag="IW651_000677"
     CDS             11936..13672
                     /locus_tag="IW651_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0813427.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13735..14571)
                     /gene="pheA"
                     /locus_tag="IW651_000678"
     CDS             complement(13735..14571)
                     /gene="pheA"
                     /locus_tag="IW651_000678"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0813428.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14568..15077)
                     /locus_tag="IW651_000679"
     CDS             complement(14568..15077)
                     /locus_tag="IW651_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0813429.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15070..16365)
                     /gene="aroA"
                     /locus_tag="IW651_000680"
     CDS             complement(15070..16365)
                     /gene="aroA"
                     /locus_tag="IW651_000680"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0813430.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16378..17469)
                     /locus_tag="IW651_000681"
     CDS             complement(16378..17469)
                     /locus_tag="IW651_000681"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0813431.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17486..18652)
                     /gene="aroC"
                     /locus_tag="IW651_000682"
     CDS             complement(17486..18652)
                     /gene="aroC"
                     /locus_tag="IW651_000682"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0813432.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18654..19718)
                     /gene="aroB"
                     /locus_tag="IW651_000683"
     CDS             complement(18654..19718)
                     /gene="aroB"
                     /locus_tag="IW651_000683"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0813433.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19751..20773)
                     /gene="aroF"
                     /locus_tag="IW651_000684"
     CDS             complement(19751..20773)
                     /gene="aroF"
                     /locus_tag="IW651_000684"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0813434.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21432..22406)
                     /locus_tag="IW651_000685"
     CDS             complement(21432..22406)
                     /locus_tag="IW651_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0813435.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22387..24270)
                     /locus_tag="IW651_000686"
     CDS             complement(22387..24270)
                     /locus_tag="IW651_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0813436.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24288..26513)
                     /locus_tag="IW651_000687"
     CDS             complement(24288..26513)
                     /locus_tag="IW651_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0813437.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26628..27662
                     /locus_tag="IW651_000688"
     CDS             26628..27662
                     /locus_tag="IW651_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0813438.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27729..28742)
                     /locus_tag="IW651_000689"
     CDS             complement(27729..28742)
                     /locus_tag="IW651_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0813439.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDLEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28820..29008)
                     /locus_tag="IW651_000690"
     CDS             complement(28820..29008)
                     /locus_tag="IW651_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0813440.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29448..29576)
                     /locus_tag="IW651_000691"
     CDS             complement(29448..29576)
                     /locus_tag="IW651_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0813441.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29579..30952)
                     /gene="rlmD"
                     /locus_tag="IW651_000692"
     CDS             complement(29579..30952)
                     /gene="rlmD"
                     /locus_tag="IW651_000692"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0813442.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31063..32118)
                     /locus_tag="IW651_000693"
     CDS             complement(31063..32118)
                     /locus_tag="IW651_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0813443.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32128..33558)
                     /gene="gatB"
                     /locus_tag="IW651_000694"
     CDS             complement(32128..33558)
                     /gene="gatB"
                     /locus_tag="IW651_000694"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0813444.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33555..35027)
                     /gene="gatA"
                     /locus_tag="IW651_000695"
     CDS             complement(33555..35027)
                     /gene="gatA"
                     /locus_tag="IW651_000695"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0813445.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35027..35332)
                     /gene="gatC"
                     /locus_tag="IW651_000696"
     CDS             complement(35027..35332)
                     /gene="gatC"
                     /locus_tag="IW651_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0813446.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35438..37474)
                     /gene="ligA"
                     /locus_tag="IW651_000697"
     CDS             complement(35438..37474)
                     /gene="ligA"
                     /locus_tag="IW651_000697"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0813447.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37491..39725)
                     /gene="pcrA"
                     /locus_tag="IW651_000698"
     CDS             complement(37491..39725)
                     /gene="pcrA"
                     /locus_tag="IW651_000698"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0813448.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39880..41439
                     /locus_tag="IW651_000699"
     CDS             39880..41439
                     /locus_tag="IW651_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0813449.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41550..43481)
                     /locus_tag="IW651_000700"
     CDS             complement(41550..43481)
                     /locus_tag="IW651_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0813450.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43499..44413)
                     /gene="pfkB"
                     /locus_tag="IW651_000701"
     CDS             complement(43499..44413)
                     /gene="pfkB"
                     /locus_tag="IW651_000701"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0813451.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44410..45162)
                     /locus_tag="IW651_000702"
     CDS             complement(44410..45162)
                     /locus_tag="IW651_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0813452.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45322..45531)
                     /locus_tag="IW651_000703"
     CDS             complement(45322..45531)
                     /locus_tag="IW651_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813453.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45542..46870)
                     /locus_tag="IW651_000704"
     CDS             complement(45542..46870)
                     /locus_tag="IW651_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813454.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVHYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        13054 a        10156 c         8368 g        15366 t
ORIGIN      
        1 taaaatatga ggaactattt ttgtttggct gttcttatta cttgatgcag ggcagctttt
       61 tcacaacctc ttattcacgg tgtttcagaa gtagcttctc ggaaataagc cgaaatttca
      121 caaaaatttg agaaactatt tttaggaaac ttcttcttat ttcttaaagc tgaccacttc
      181 tatcacaacc tctttttgac tatgcattca catcgatcac tttagaatca tcattgatga
      241 catcgcgatc taaatctttg atgcgtgcac ttgtcacgat accagctacc atgctaccgc
      301 tgacgtttac tgctgtacgt gccatatcga ttaatggttc aacagagata actaacccaa
      361 cgattgctac tggcaagttc attgccccta aaacgatcag tgaagcaaat gttgcgcccc
      421 caccgactcc agctacacca aaagaactga ttgttacaat tgccacaagc atcaaaataa
      481 attccaaact aaagacattc actccagctg ctggtgcgac gatcgttgcc aacatagctg
      541 gataaacgcc agcacagccg ttttgtccaa tagacaaacc aaaacttgct gagaaattag
      601 ctgttgcatc atctactcct aaagcttttg tctgagtctc gatattcaat ggcaaagctc
      661 cagcacttga acgtgaagta aacgcaaagc tcaataccgg aaaggctttt ttgaaatagt
      721 tgattggatt gacttttgca ccaatcaaaa tcagtgagtg gacaatcaaa acagcaagca
      781 atgctacgta agaagcaacg acaaatttac ctaaattgac gatagctgca aaatcacttg
      841 ttgccaaaac gtttgtcatc aaagcaaata ctccataagg tgtcaaacgc aaaacaagcg
      901 tcacgatccg cataatgatt ttatacagac tgtcgactaa atctgaaaag aatgctgctt
      961 cttttggcgc ttttcttttc acgcctagat aagcgattcc gacaaatgcc gcgaaaataa
     1021 cgactccaat cgtacttgtt gcacgactac ctgctaaatc agcaaaaacg tttttaggga
     1081 taaaggataa aatctgctgt ggaatcgata agttttcaac tgtttcctga cgtgttgcca
     1141 gttcagagat tcgtgctgtt tctgcagctc cttgtgtgaa tttagctcct tggagtccga
     1201 acagcattgt tgaaccaatt ccgatcaatg ctgcaatagc tgttgttcca agtaaagaag
     1261 tcaatacagt cgcactaatt ttccctagct tttcagaaac tttcattttt gtgaatgcac
     1321 caatgatcga aataaacact aaaggcataa ctagcatttg caagaatgat acatatcctt
     1381 gacctacaat cccgatccat tccattgatt gagtaacgac tttgtcgtct acaccaaaga
     1441 tcaattgaag cagaccgcca aagacgatac caactcctaa tgctgtaaag acgcgtgttg
     1501 aaaattttgt atggcgcttt tgcatacgat agaacacata taataatgcc gcaaaaacta
     1561 gaaccactaa aactgtgatg agtgttgtca tttttctcct cctcatatta ttaatgacaa
     1621 gtccatcgaa ggaagtaaag gttcccgaga atgattcttc aaccatcctc ctgaaaaaat
     1681 ttttttcagt gacttttata ttttagacga tttttatcga aatgacattc tttttgctta
     1741 caattagtaa taaaaaaaca tacctgtgat agctttaatc tatcgcaggt atgtttttac
     1801 tgtctattct tgtttttctg attgaaaatt caattttacc ccaggctgat ctaaggcgat
     1861 ctctgtcacc tcataagaca ttcggcgtac gatatcgaat aacggtgcta ctagctcgtc
     1921 cacttcttct tgtgtgacat cattttgacc ttcaattttc cgattgagga tatgattgat
     1981 ctgactcaca cttccagata aaacatatgt atcaaacact aaacggaact ctaagcgtgc
     2041 tcctacgatg ctatgttctt tcggatagtt ttcgatcgtt tcttttaaag gcgagaagcc
     2101 tactttcaaa tctgtttcta cttcttggtt cggcaatctc atatcataat gaaatgcttc
     2161 tacaacttct ttttgtcttc tgatttccat ataattttat cactccttta tgaaaattat
     2221 aacatatctc ctttgaaagg ggatactact ttttaatgat gctctgcaag gtaaggattt
     2281 cattcagatt tcctaccaca taatctgccg ggatctcacc cagaaaatga tcgatgtcgt
     2341 aaagacaagt agcgactcct gcatttttcc ctgcttcgat atctaaacga cgatcgccga
     2401 tcatcatcgt gtctgttctt tctaaatgaa aagtatcgat caaataattt aaagatgctg
     2461 gatcaggttt acgaggaaag tcctgatcga tccctactac ttcctcaatc agatgtgcca
     2521 atcgatgttc ttttaacagg ccccatgttg attctgtcaa acggtgggtc aggatgaaat
     2581 gacgaccgcc gttgtctttt agcatttcca gtacttgttt cgtttctgga aatggacggg
     2641 aaacttccgt actttctttt tcatatcggt gaaataattc attaaactct ctggcattca
     2701 agtttgattc attgatcaaa taactagtag aatacttttt cattttgaag tagatttctt
     2761 ttttgtccat actgatacca aaatctttta gtgcttgcca tgcaccatct accattgcgg
     2821 gatacgtatc aaacaatgta ccatcaaagt cccatatata atttttcatg ttccttttgt
     2881 tccattcctt tgcttctttt ttctatctct cacttttaga taaaggtgtt ctttaaagta
     2941 tttttttgtc cagaattgtc aaatcatctg ctaatgtata ctggattggt tgtgcatttg
     3001 ggatatcgat cgtatccatt tgatcttcag gaacatcttc taaatatttt gtcagtgctc
     3061 ggatgctatt accatgaccc acaactagta aatcttgccg atcttttagt tttggtgcta
     3121 gatgatcttg ccataaggga atgatccgat tcactgtcat ttccagactt tctccgtatg
     3181 ggatcaaatg cggatccaag tgatcatatc tgcggtcaaa tctattttct gcggttaacg
     3241 gcggaacttc cgtatatcct ctgcgccatg tttttacttg atcttttccg tatatttctt
     3301 tcatcttatc tttgttttta cccacaagat ccccataatg ccgttcattt agacgccatg
     3361 tttttattat aggaagccat aactgaccca tttcttctag tgcgacttgt ccggtaagaa
     3421 tcgcacgttt caaaacagag gtataaacgg catcgaattg aagtccggct tcaagcattt
     3481 tttgaccggc aactgctgct tgttcttttc ctttttctgt taaggcgaca tctagccatc
     3541 ctgtccagta attttcaaaa tttgcttcgc tttcgccatg acgtaataat actaatttca
     3601 ttcttttctc ccttaaacat tcgtttcgaa ttttatacca catgttttgc atgtcacacg
     3661 tattcgtcct tttcctttag gtgcacgctg cttttgatga caattcgggc actcaaaaaa
     3721 agtataactt tttcgatcca ttttgacttg ctttttgttt tttatctgat gatatttttc
     3781 tttgactcta tcaagatatt tcaaataacg ttgattctca tttagtctgg ggtagatttt
     3841 ttttgatagg aaacgaaata tcatccatcc gaaaaagacg atgaacaaca attgtccgat
     3901 tctatagggc aaccaacgac ttagcaaact aaagacaagc gcagcgatca atagaccgcg
     3961 attcaactga tcaaatttcg catatcttcc ctgcataagt ttgtgatagt tctgctgaaa
     4021 tcgtattagc ctttctaacc atgtttgcat catattatct cctccatcat ctaatattta
     4081 ttatgacaaa aaaatccaaa aagaaaaagc cttaattggc cttttccatt gcaacttggt
     4141 gaaggacatt ccgatttctg gtaaagtcaa tcacaatcac tttcttttta tcttctctgt
     4201 tgactactcg aaatgtgtcc gtcaatggaa gaaacagctt cttagatggt agctgttgca
     4261 tacgtataaa acgatggatc atcgtaatca attgaggaaa ttgaatagac gtgtgaagca
     4321 gacctggata ttttttcaac agctgtgtcg ttttgatcag attccgtttt ttactgatca
     4381 cttttaaaaa gctttttaga atcttttgct gacgtctaaa aatagaaaaa gaattttcgt
     4441 catctatttg ataacgcatt tgtttttgca gttctgctgc agtcagatgg atcttcccat
     4501 cgtttgtatc gatcattaaa ccttctggaa acagttcaac cactgctcga tcccaagatt
     4561 caagagaaag ctcgatataa tcttttactg gtaattccaa ttctacctta aaaaagtgca
     4621 aaacagccga cagcccttct gcttgataga tttctcctaa tgttagcccc tctagatgga
     4681 actctggtga aaaatgcagc aatgttttat tttcctgcaa ttgaacatat agaaatgtag
     4741 atcgctcaat cagagtatct tttaaaacaa ctaaaaaatt catttttctc cccctttttt
     4801 catcattttt aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat
     4861 aaaaatttac tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac
     4921 gctcatccgc cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac
     4981 ttttctctat cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga
     5041 tgaatcttag tgataccaat gactttgtct acatcaatac taaaacaaat accattacct
     5101 ggaatatcga tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg
     5161 ggtactaatg tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct
     5221 gaatcatgta caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct
     5281 tttgaaaagt cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga
     5341 agattcaatg ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt
     5401 cgattctgtc tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata
     5461 ttggtgtcaa tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt
     5521 caatttgtgc agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg
     5581 ttgctactcc accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta
     5641 aaataaatgc tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca
     5701 aacgccacat agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga
     5761 ttttcgatcg gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg
     5821 ccggctcagc aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat
     5881 tgtaaggcaa tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat
     5941 tgactccgtg caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt
     6001 caaagaaacg ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta
     6061 cgataggtgc cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt
     6121 gctgccagaa actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa
     6181 cgaagcaata ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact
     6241 agtcatccct ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc
     6301 ccccgaatca aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac
     6361 gcctatgtag cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag
     6421 cgcaaaggct agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc
     6481 aatctctccg tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc
     6541 aatcgtgatc acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt
     6601 tttgatcata tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa
     6661 caaaaagagc gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc
     6721 ttctccttct aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa
     6781 tatagcagca atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa
     6841 gtttacctta aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt
     6901 ctcaacataa aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa
     6961 ggaacgcgct tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct
     7021 tttgcaaaac aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga
     7081 tcattccata gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc
     7141 gtttatcagg agattgcaaa gctactaagg gccataaata attgttccag ctactcatga
     7201 atgtgatgat tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa
     7261 aaatacttag ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag
     7321 atttaacatt ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga
     7381 caaccaagaa actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata
     7441 aagggatcat caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta
     7501 ggtcttttcg tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca
     7561 atgcaaacac agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat
     7621 tcagctcatt ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt
     7681 ttcccgatgt gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga
     7741 aaatggaaat aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg
     7801 aacgaagttc taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca
     7861 tatttttcat cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa
     7921 ggaaacaata acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact
     7981 caaattatag atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat
     8041 attttgtact tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa
     8101 aatgattggt tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc
     8161 gtcaatagat gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa
     8221 aatcatattg tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca
     8281 aattggatga gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt
     8341 gtttacaagg ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga
     8401 agtgatacat ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc
     8461 atttaaaagg ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa
     8521 atacaggaat gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata
     8581 attacctaac ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa
     8641 agccaaaacc atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa
     8701 agcatgcccg tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt
     8761 gattatctac atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg
     8821 tcttttcgtt tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag
     8881 ccaattgtgc ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat
     8941 tgatgattcg atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt
     9001 aattcacttc tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac
     9061 tgtaaaattc agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata
     9121 ctagtccgat ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt
     9181 ctaggaaatc tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc
     9241 cgccaaggtt agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa
     9301 tcttccattt tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag
     9361 ttgggctaga agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg
     9421 tttgttcgac tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag
     9481 atgcattgtc ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct
     9541 gttgcataat catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc
     9601 ccgtcttttc tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca
     9661 tatcctcttc tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta
     9721 cgcccgaatc gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat
     9781 acggtgcaaa atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag
     9841 aagtcaaata gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg
     9901 tattcccgct cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa
     9961 caacttctat ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga
    10021 tattgaatgt ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac
    10081 tagcacttcc gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa
    10141 ttaactttct gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg
    10201 ctacattcat cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat
    10261 ttctcctaaa ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc
    10321 tagaccaaag atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc
    10381 tagaaataag tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata
    10441 cgcgtaattc ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat
    10501 tggtctgagc acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat
    10561 ttttctttgg tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat
    10621 ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc
    10681 tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat
    10741 cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa
    10801 aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag
    10861 aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa
    10921 cacaccttgt ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta
    10981 aacaaaaaca gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc
    11041 ctgtttctac cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac
    11101 ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt
    11161 ctgattcttt tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca
    11221 aaggacaacg tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt
    11281 tattaaccaa ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact
    11341 acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg
    11401 atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg
    11461 ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat
    11521 tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac
    11581 tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg
    11641 agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc
    11701 atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa
    11761 aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta
    11821 ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt
    11881 ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag
    11941 aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct
    12001 ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat
    12061 ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag
    12121 tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag
    12181 ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca
    12241 aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt
    12301 tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc
    12361 tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct
    12421 ttttgcacac cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa
    12481 aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa
    12541 gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat
    12601 gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat
    12661 gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg
    12721 tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc
    12781 taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc
    12841 tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga
    12901 ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca
    12961 tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta
    13021 tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc
    13081 acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca
    13141 gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac
    13201 gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta
    13261 catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga
    13321 tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta
    13381 tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa
    13441 tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa
    13501 acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct
    13561 aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt
    13621 aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc
    13681 caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct
    13741 acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag
    13801 catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc
    13861 gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa
    13921 gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc
    13981 aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg
    14041 gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa
    14101 gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt
    14161 cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac
    14221 attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag
    14281 gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat
    14341 ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc
    14401 tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc
    14461 gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca
    14521 agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg
    14581 atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga
    14641 tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct
    14701 cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact
    14761 agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga
    14821 ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc
    14881 tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat
    14941 tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac atactctttt
    15001 tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc
    15061 agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa
    15121 tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat
    15181 caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc
    15241 gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc
    15301 tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa
    15361 ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat
    15421 gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg
    15481 actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat
    15541 cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac
    15601 attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc
    15661 tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac
    15721 tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt
    15781 atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc
    15841 aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga
    15901 taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa
    15961 gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga
    16021 agcatccccg aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg
    16081 tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct
    16141 accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg
    16201 gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt
    16261 tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat
    16321 cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta
    16381 tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc
    16441 ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat
    16501 cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac
    16561 gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt
    16621 atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga
    16681 ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa
    16741 tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga
    16801 ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata
    16861 ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg
    16921 acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt
    16981 ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt
    17041 aaagatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg
    17101 agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac
    17161 gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct
    17221 ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc
    17281 tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac
    17341 tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt
    17401 ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg
    17461 ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca
    17521 ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta
    17581 ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg
    17641 aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct
    17701 tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca
    17761 ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc
    17821 ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa
    17881 attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct
    17941 tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta
    18001 caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt
    18061 gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt
    18121 gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg
    18181 ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca
    18241 tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt
    18301 tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt
    18361 ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat
    18421 tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga
    18481 tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc
    18541 gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa
    18601 taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt
    18661 cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc
    18721 ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg
    18781 tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt
    18841 atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa
    18901 gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc atatcctgct
    18961 gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg
    19021 tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga
    19081 acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt
    19141 gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc
    19201 ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg
    19261 acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact
    19321 tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc
    19381 acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg
    19441 gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg
    19501 gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac
    19561 atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga
    19621 ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga
    19681 atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa
    19741 aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt
    19801 ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat
    19861 ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac
    19921 gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag
    19981 ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg
    20041 ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg
    20101 caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc
    20161 caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg
    20221 cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca
    20281 cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt
    20341 cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca
    20401 gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt
    20461 cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta
    20521 catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa
    20581 tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg
    20641 tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc
    20701 cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca
    20761 taattactat cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt
    20821 ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc
    20881 tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct
    20941 gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata
    21001 taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt
    21061 agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat
    21121 ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat
    21181 cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc
    21241 atttttatga aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca
    21301 attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct
    21361 tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa
    21421 tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg
    21481 taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca
    21541 aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc
    21601 agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat
    21661 cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt
    21721 tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg
    21781 gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt
    21841 tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa
    21901 cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt
    21961 ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt
    22021 tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt
    22081 actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact
    22141 tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg
    22201 tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt
    22261 ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc
    22321 cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata
    22381 tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc
    22441 ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc
    22501 aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc
    22561 ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt
    22621 catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat
    22681 gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat
    22741 gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata
    22801 atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc
    22861 ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt
    22921 ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg
    22981 tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt
    23041 cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt
    23101 ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt
    23161 ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac
    23221 cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt
    23281 acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc
    23341 caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc
    23401 atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc
    23461 atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa
    23521 atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc
    23581 ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg
    23641 gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag
    23701 aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc
    23761 ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa
    23821 atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga
    23881 accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt
    23941 cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg
    24001 gatcttgtca taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca
    24061 ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca
    24121 aaaacgccat gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct
    24181 tgttgcataa caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg
    24241 atcatctaac cattttatat ttgctttcat acttttttcc ttctctctca taactttttg
    24301 aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc
    24361 atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca
    24421 tctaacccca tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct
    24481 aaaaacacaa tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct
    24541 tcaaaaggac tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga
    24601 taggatataa tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc
    24661 agttcataac caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat
    24721 ctgtcggtct gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa
    24781 gcaaggcttg ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc
    24841 catgactgcg gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag
    24901 ccttcccata agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg
    24961 agcatataac gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca
    25021 tctgaaagcg aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc
    25081 tttctcattt gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt
    25141 tgtgatctgg aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt
    25201 cgatacagtt cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat
    25261 cctttgctat gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac
    25321 tcgaaccagt ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct
    25381 aatacgaaca tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca
    25441 ctattgaagt caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat
    25501 tgatgacgac ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg
    25561 ttcaatccct gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca
    25621 ttcaattccc acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc
    25681 tgatcttttt caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct
    25741 tctccagtat tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga
    25801 ctgctcgttc ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa
    25861 atctgtcttt cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta
    25921 tgggcaaagt ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca
    25981 tttcttatct ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa
    26041 tcgaaataaa tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac
    26101 ttgtccatca cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa
    26161 tggctgtcat atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg
    26221 ggcgttcgaa agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac
    26281 agagaccggt caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa
    26341 tttttattgt ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg
    26401 ctttcttcta tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc
    26461 ttttcatcaa ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca
    26521 ctcccaacaa aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa
    26581 caattttgtc aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta
    26641 gagatgtcgc acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg
    26701 atcagacgat ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt
    26761 tggattatga catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga
    26821 tctctacgat cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag
    26881 ggatggaaga agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg
    26941 ataatcctaa acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg
    27001 ttacgaaaga atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata
    27061 accctgacat ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa
    27121 ccgtactgga attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata
    27181 aagtcgatat ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg
    27241 cctatcagca ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg
    27301 gctggacaga aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc
    27361 ttcctactgc cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga
    27421 aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga
    27481 caatcgcaaa cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc
    27541 aagcagctgt tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt
    27601 tgacttatcc tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt
    27661 gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc
    27721 acaacctctt acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat
    27781 tcttttagcg tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa
    27841 aacaaggatt gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc
    27901 tcctggaatg agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca
    27961 tggtcagcca cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc
    28021 aacgaccacg caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt
    28081 gctgttagct tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt
    28141 ggaagtagct gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca
    28201 tattgcaatg cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct
    28261 agcaatttgt cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga
    28321 acttgattag ggtattctaa atcaggcgct aattcataat tcatcgcaac cactgcgatg
    28381 ttggcgtctg aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg
    28441 aatcctccac catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag
    28501 tagatgtcat aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc
    28561 acgttttttt ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac
    28621 atatggttga tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca
    28681 aacgcgatcc caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc
    28741 ataaggctcc ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta
    28801 tcaagctctc ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca
    28861 aaagaaattt tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc
    28921 cacattgagg acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc
    28981 ggtcaatcac ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag
    29041 acaactaatt tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta
    29101 tcgtattcta aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc
    29161 gtcatccccc ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt
    29221 agtgttgaat catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa
    29281 ttgaaataac gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc
    29341 acaactcaac agtccttatt ggcaaatcgc caaaataatg tagactaact aaaaagacta
    29401 agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta tttattaaag
    29461 attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat ttgtttggat
    29521 cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg gtccattttt
    29581 attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg aaaagatcta
    29641 caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca tctcttgcaa
    29701 aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg attgctgatt
    29761 caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca gatggtttga
    29821 tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca taatgaacat
    29881 tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct ggaataactt
    29941 ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta cctattcctg
    30001 aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg gctgtttggt
    30061 acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag attcgatagg
    30121 tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt tctttgtctc
    30181 ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc acttctggca
    30241 attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct ttacgagtaa
    30301 ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg tgacgaagct
    30361 gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag atatctctga
    30421 ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg atagggacta
    30481 gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata cggcgaacag
    30541 ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt ggaagtacag
    30601 gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt tgtttgaaga
    30661 caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga agtaatgcat
    30721 tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact tttccatatc
    30781 caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg agtgcatttt
    30841 cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa ctcaagtcga
    30901 cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc atattttgct
    30961 cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa ttacacaact
    31021 aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct tttgattagg
    31081 tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac gttcgacttc
    31141 ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg tgattgcttc
    31201 atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat tttggaaatg
    31261 catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc gattgatatc
    31321 atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg gatcatcgac
    31381 atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga tatttgctgt
    31441 cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg cgatctgttc
    31501 gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc cgatatattc
    31561 cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca tctctgctcc
    31621 tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa cttcataagt
    31681 caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat cagaaaggcc
    31741 gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt taatattatc
    31801 tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga tgatggccat
    31861 tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg tgccatcgcc
    31921 accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt cattcttggc
    31981 tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt cttctaatgc
    32041 ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg ggttataaat
    32101 tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt taagtagctt
    32161 attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac cgactaggaa
    32221 gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt tgttatccaa
    32281 aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc aaccattttt
    32341 ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga cttttttggc
    32401 gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta agttctcagg
    32461 tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag aaacttcacc
    32521 catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag cttcaaaaaa
    32581 atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa gtccaaactc
    32641 acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt ttactttttg
    32701 aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt aacgataatc
    32761 actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt caaaacggcg
    32821 tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt tttcttcaaa
    32881 agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg ctttcgtacc
    32941 gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca tagagccttc
    33001 ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg cttctagata
    33061 agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga tcaaaggcgt
    33121 gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt tttttccagc
    33181 atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt ctacttcgat
    33241 ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat atgcttttgg
    33301 attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga tttcacagtt
    33361 taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta ctggcagcac
    33421 gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac caaaatgagc
    33481 tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt ctagtccaat
    33541 gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg tttatggaat
    33601 tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc aaaataatta
    33661 ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac agacatacct
    33721 ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat tgtgattgga
    33781 tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc gatgatcaag
    33841 tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac ttgtcctgct
    33901 tttttgaagt aagcgtcata ataacccgcg cttaaggaga atgtacctaa catgatacgg
    33961 cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc atctaatgat
    34021 ttcacatttt cagagcgata accgtagcgg ataccatcga atcgttgcaa attggaactt
    34081 gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg aggcaagctg
    34141 acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa tacagcttct
    34201 tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat cttcattcct
    34261 ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga agaagtaccg
    34321 tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt caccgtacgg
    34381 gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa acgtgagaca
    34441 cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg gcggatactt
    34501 cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc tgctgcagaa
    34561 ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt tttcttgaaa
    34621 taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt accaacaggg
    34681 atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg ttcaaaattg
    34741 taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt atccttgata
    34801 ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc taaagctttt
    34861 gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cctcgacctc aggctctgtt
    34921 tctttgattc gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc tttcgataca
    34981 agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat gcgccagcct
    35041 ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc ggcacatttt
    35101 tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc acgtttactg
    35161 tttcaaccac attcgacgta aatggaacac cttccgtatc tacttcttcc agcatttcaa
    35221 ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca tctgcgaacg
    35281 caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc atactacccc
    35341 ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg ccgtgtctga
    35401 aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact gagcgacttt
    35461 tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat tcttgtgctt
    35521 tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt ttcgatacgc
    35581 taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca cgagtaaatc
    35641 gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg acttcttgca
    35701 attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt aattcattca
    35761 tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca atgatatcac
    35821 cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt tctagatcac
    35881 cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt cctaacccga
    35941 aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa atattatttg
    36001 ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat ttgtaaagat
    36061 cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc ggccccaacc
    36121 catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc atttgagcag
    36181 ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca agttcacttc
    36241 cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt cgtttatcta
    36301 acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga actgtatctt
    36361 tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca cggcttactg
    36421 tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc cctgttcgac
    36481 cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga aacttgtaag
    36541 ctgctgccca tctcggtgct ttgatagtaa aaccaagttg atcttgtagc gagaattcat
    36601 tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg tcatgatatt
    36661 cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc cgattcgtgt
    36721 ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct tgcataggac
    36781 cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg attttagaat
    36841 ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata tcttttccct
    36901 ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa cattcgcctc
    36961 ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca gtttttaggt
    37021 tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc acgaaatttc
    37081 catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag caatagtcaa
    37141 ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct tctttgctga
    37201 aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa ccttctaata
    37261 cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga tattgttcct
    37321 cgattgctac taattctgcg tattctttat cataaacata gtcttccact gttggtttat
    37381 cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct tctgctcgtt
    37441 tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta ctaagctttt
    37501 tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa agccacatct
    37561 agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc ccattttttg
    37621 tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc acttttagag
    37681 ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc gaatacaggc
    37741 gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc gatttcttct
    37801 acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga gaaagcattt
    37861 gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg gcgttcttcc
    37921 tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc ttctagacca
    37981 atcaggaata caactggaaa ttctaatcct ttggctgcat gcaaagtcat caatgtcact
    38041 tgggaagcat cttcttctaa attgtcaatg tcggaaacaa gtgccagatc atttaagaag
    38101 accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc gaattgtttg
    38161 tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc ttgtgcttct
    38221 aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac ctctttcgtc
    38281 aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat ctctccaaat
    38341 gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt ttgcgctgct
    38401 tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat agatcctggt
    38461 ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc ttgaggattt
    38521 accagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta gaacttgtgc
    38581 cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat tacacgagat
    38641 tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt gcttcgaatc
    38701 tcttcttgca tccggtctac gataaaacgg gtttcgtctc gttcattatc tgcacggtag
    38761 taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt tcgattgcta
    38821 ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg atagttttgt
    38881 tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat gttctgcata
    38941 tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca caggttccta
    39001 aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc ttggtactca
    39061 tctacatgaa tataatggaa tttgttttga taatacgtga gtgaatcagg atgttcttca
    39121 aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt atattgaagc
    39181 tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact gccttgcatt
    39241 tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt tcctaatata
    39301 cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg tttcatcaag
    39361 gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat aaaatcaaca
    39421 tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac atcttcgcct
    39481 ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt attggtaaaa
    39541 gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata ggcaatgcgg
    39601 tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa tggtccgtca
    39661 gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc agctttggat
    39721 gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt cttggctttc
    39781 attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct tgtcaattcg
    39841 ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa aggaaaaatc
    39901 aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt tggactgctg
    39961 acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga tttagttcga
    40021 agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc tatttgctgc
    40081 atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat caagggaagc
    40141 gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg gttctctgtt
    40201 ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt tctaggtaga
    40261 gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca attgtttcgg
    40321 agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg acttgcagca
    40381 gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca tcactccttt
    40441 tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt cgtctctctt
    40501 tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt gccgttgaaa
    40561 tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt tatttatcag
    40621 aatgtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc ttattttcac
    40681 gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag tactctaggc
    40741 ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat tatgattatc
    40801 ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggggg tcttccaggg
    40861 gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta tggaaacatt
    40921 atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat tgccatggct
    40981 ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt gacagaaatg
    41041 gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc ttatatcgtc
    41101 aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct acttggaaat
    41161 actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt aacggcagag
    41221 agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat gctgctaatc
    41281 tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat gaatgtagga
    41341 gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca ggaaatcgag
    41401 gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa aaactatttt
    41461 acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa agaagaggct
    41521 gggaaaaccc agcctcttct tttttatttc tatgcttctg ctactgcagg agcttttctc
    41581 aataaaccat aaatcacacc agatacgact gctccgatag caataaacac caaatacatg
    41641 accgggtgac tcagtaagaa tacaacgaaa atcccgccgt gaggagccag taatttgatt
    41701 ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact tggaatggca
    41761 cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga tagtcccatc
    41821 acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt aaacaaacgt
    41881 gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga agccatcacg
    41941 acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac ataagcggct
    42001 ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc accaagtaaa
    42061 gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt gattgctttc
    42121 atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa cactggataa
    42181 aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa tttacgtaaa
    42241 aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa tcctgcaccg
    42301 cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg gtcaccgata
    42361 cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc ttgtccgatt
    42421 tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg atcatgtggc
    42481 acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc accaataacg
    42541 aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg tccgatagta
    42601 ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa tactggtgca
    42661 gtaccactaa gggctagatt gatcaactct tctggtttac ggataccatc actgacagga
    42721 cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc tgctgcaacg
    42781 attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat tccttctgaa
    42841 ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt caaagcatct
    42901 tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac aacgaatttt
    42961 ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt ttcttctgct
    43021 tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt atttgcttct
    43081 tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc taatgcttgc
    43141 aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata agttggctgt
    43201 ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac tgttgcttct
    43261 ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc tgtagaagtt
    43321 tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat ccggccggca
    43381 tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc ttgcaaatcc
    43441 atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct ccttgaaact
    43501 aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg cgagctgcca
    43561 agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag gcttcgattg
    43621 gatctttcgt acggctgtaa gccccaataa agccagcaat cattgaatcc cctgcaccta
    43681 ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt tccgtgaaga
    43741 gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca gctagtaatt
    43801 ttttcccata cggaatgatt tcttctacag attggaaggt tgtgttgtaa agctcagcta
    43861 attcgtgatt gtttggtttg atcaacaacg gtttcttaac aagtgcattt tttaggtcat
    43921 cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca atcagttctt
    43981 gataaaatcc ttttcgcagg ctagctggcg cgcttcctga caatacgacg atatctcctt
    44041 gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa atagatgggc
    44101 ctaagccatt gatctcagtt tcagtgtctg attttagttt gatattgatt cttgtatctt
    44161 cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc gaaataaatt
    44221 cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc cgtttcaaaa
    44281 tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc attcggttca
    44341 aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga ttaagtgtta
    44401 ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt cattaacgaa
    44461 ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa cagaggtgaa
    44521 ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg aatttttcat
    44581 ggctaaacgt ttaacagctg cctcttctgg atccggagtc gtgaatcctt gttcaagatg
    44641 ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt gttctatcgc
    44701 attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa taatggtagc
    44761 aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta ctgtgatctg
    44821 ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta aataaatgac
    44881 atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt cgtgaacgtt
    44941 tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt tcttagctcc
    45001 gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac gtcttatcgt
    45061 tgattcagaa gcattcaaaa ggtttacaag atcttgtgat ttcacgattt tctgctgttc
    45121 aagcaagtct aggatttttt gtctgcgttc ctctgtaagc attttcacga tcacctcaat
    45181 acaaataata gcatattttt tttctactag caatataaat cagtcaaaaa cgttcataaa
    45241 aattataaat cagtcaaaaa aaaaataaaa aaccagtcag aaagactggt tttttatttt
    45301 tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt ttttcatata
    45361 aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca tcccgccaaa
    45421 catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa agagacagac
    45481 ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca ttttcttcac
    45541 ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa aattgcacac
    45601 gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt ttctttattc
    45661 gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat tctgacaggg
    45721 attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt tcatctgtta
    45781 cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca ttatccaaag
    45841 caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg tcaacccaag
    45901 gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa tctccttgtt
    45961 ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaacg ggttgcattt
    46021 gtgcattctg ataatggaca gattggatcg tatggtcagt gtaaatgtat tcaacgaatg
    46081 gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca agctggtccc
    46141 taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg tctcgataaa
    46201 gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt tggatgtcga
    46261 gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt tcccaacacg
    46321 aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg caatcgacta
    46381 ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc tttttctcaa
    46441 acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta taagctcgca
    46501 tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga atcaaaaatt
    46561 gcttttcttt gaaaacagag atagacaata cattttttat caataattga ttatcttcca
    46621 agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg tctcttaccg
    46681 atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct tgttgtgtga
    46741 aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt
    46801 cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat aatttatctt
    46861 cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa ccaatgataa
    46921 agaaaaacta aaactttatg tctt
//
LOCUS       DACEJR010000011        45192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEJR010000011 DACEJR010000000
VERSION     DACEJR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.011"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..2095)
                     /locus_tag="IW651_000705"
     CDS             complement(23..2095)
                     /locus_tag="IW651_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0813455.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IW651_000706"
     CDS             complement(2028..2405)
                     /locus_tag="IW651_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0813456.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IW651_000707"
     CDS             complement(2534..3094)
                     /locus_tag="IW651_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0813457.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IW651_000708"
     CDS             3244..4536
                     /locus_tag="IW651_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0813458.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IW651_000709"
     CDS             complement(4596..5714)
                     /locus_tag="IW651_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0813459.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW651_000710"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW651_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0813460.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW651_000711"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW651_000711"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0813461.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IW651_000712"
     CDS             8920..9519
                     /locus_tag="IW651_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0813462.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW651_000713"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW651_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0813463.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IW651_000714"
     CDS             complement(11214..11558)
                     /locus_tag="IW651_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0813464.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IW651_000715"
     CDS             complement(11695..12831)
                     /locus_tag="IW651_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0813465.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IW651_000716"
     CDS             complement(13020..13994)
                     /locus_tag="IW651_000716"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0813466.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IW651_000717"
     CDS             complement(14074..14949)
                     /locus_tag="IW651_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0813467.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IW651_000718"
     CDS             15079..16986
                     /locus_tag="IW651_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0813468.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IW651_000719"
     CDS             complement(17527..20805)
                     /locus_tag="IW651_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0813469.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IW651_000720"
     CDS             complement(21076..21621)
                     /locus_tag="IW651_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0813470.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IW651_000721"
     CDS             complement(21633..22325)
                     /locus_tag="IW651_000721"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0813471.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IW651_000722"
     CDS             complement(22344..22628)
                     /locus_tag="IW651_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813472.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IW651_000723"
     CDS             complement(22641..23204)
                     /locus_tag="IW651_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0813473.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IW651_000724"
     CDS             23432..24094
                     /locus_tag="IW651_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0813474.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IW651_000725"
     CDS             24218..25414
                     /locus_tag="IW651_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0813475.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIAFTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IW651_000726"
     CDS             complement(25515..26891)
                     /locus_tag="IW651_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0813476.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IW651_000727"
     CDS             complement(26928..27794)
                     /locus_tag="IW651_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0813477.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IW651_000728"
     CDS             complement(27883..28908)
                     /locus_tag="IW651_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0813478.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IW651_000729"
     CDS             complement(28926..29600)
                     /locus_tag="IW651_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0813479.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IW651_000730"
     CDS             complement(29784..30677)
                     /locus_tag="IW651_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0813480.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IW651_000731"
     CDS             complement(30751..32448)
                     /locus_tag="IW651_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0813481.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IW651_000732"
     CDS             complement(32441..33754)
                     /locus_tag="IW651_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0813482.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37272)
                     /locus_tag="IW651_000733"
     CDS             complement(33754..37272)
                     /locus_tag="IW651_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0813483.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37565..38023)
                     /locus_tag="IW651_000734"
     CDS             complement(37565..38023)
                     /locus_tag="IW651_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0813484.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38321..38839)
                     /locus_tag="IW651_000735"
     CDS             complement(38321..38839)
                     /locus_tag="IW651_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0813485.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(38841..39458)
                     /locus_tag="IW651_000736"
     CDS             complement(38841..39458)
                     /locus_tag="IW651_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0813486.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39455..40309)
                     /locus_tag="IW651_000737"
     CDS             complement(39455..40309)
                     /locus_tag="IW651_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0813487.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40306..41217)
                     /locus_tag="IW651_000738"
     CDS             complement(40306..41217)
                     /locus_tag="IW651_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813488.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41258..42592)
                     /locus_tag="IW651_000739"
     CDS             complement(41258..42592)
                     /locus_tag="IW651_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813489.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42800..43630
                     /locus_tag="IW651_000740"
     CDS             42800..43630
                     /locus_tag="IW651_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0813490.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43874..44123)
                     /locus_tag="IW651_000741"
                     /pseudo
     CDS             complement(43874..44123)
                     /locus_tag="IW651_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44314..44508)
                     /locus_tag="IW651_000742"
     CDS             complement(44314..44508)
                     /locus_tag="IW651_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813491.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        13071 a         9721 c         7969 g        14431 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg ttaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta tcattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttccttttct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc atttaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ttgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    37561 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    37621 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    37681 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    37741 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    37801 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    37861 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    37921 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    37981 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    38041 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    38101 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    38161 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    38221 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    38281 atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata
    38341 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    38401 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    38461 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    38521 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    38581 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    38641 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    38701 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    38761 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    38821 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    38881 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    38941 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    39001 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    39061 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    39121 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg atagtcgatc
    39181 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaag agagaagttc
    39241 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    39301 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    39361 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    39421 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    39481 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    39541 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    39601 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    39661 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    39721 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    39781 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    39841 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    39901 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    39961 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    40021 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    40081 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    40141 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    40201 tccaaagcaa tagaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    40261 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    40321 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    40381 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    40441 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    40501 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    40561 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    40621 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    40681 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    40741 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    40801 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    40861 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    40921 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    40981 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    41041 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    41101 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    41161 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    41221 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    41281 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    41341 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    41401 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    41461 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    41521 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    41581 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    41641 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    41701 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    41761 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    41821 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    41881 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    41941 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    42001 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    42061 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    42121 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    42181 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    42241 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    42301 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    42361 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    42421 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    42481 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    42541 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    42601 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    42661 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    42721 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    42781 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    42841 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    42901 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    42961 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    43021 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    43081 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    43141 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    43201 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    43261 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    43321 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    43381 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    43441 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    43501 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    43561 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    43621 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    43681 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    43741 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    43801 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    43861 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    43921 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    43981 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    44041 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    44101 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    44161 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    44221 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    44281 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    44341 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    44401 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    44461 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    44521 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    44581 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    44641 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    44701 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    44761 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    44821 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    44881 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    44941 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    45001 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    45061 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    45121 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    45181 tttagaattt tt
//
LOCUS       DACEJR010000012        44842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEJR010000012 DACEJR010000000
VERSION     DACEJR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.012"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            212..604
                     /locus_tag="IW651_000743"
     CDS             212..604
                     /locus_tag="IW651_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0813492.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IW651_000744"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IW651_000744"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0813493.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IW651_000745"
     CDS             3165..4775
                     /locus_tag="IW651_000745"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0813494.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IW651_000746"
     CDS             5067..5936
                     /locus_tag="IW651_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0813495.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IW651_000747"
     CDS             6101..7372
                     /locus_tag="IW651_000747"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0813496.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IW651_000748"
     CDS             7369..8664
                     /locus_tag="IW651_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0813497.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IW651_000749"
     CDS             8763..9026
                     /locus_tag="IW651_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0813498.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IW651_000750"
     CDS             9471..9920
                     /locus_tag="IW651_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0813499.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IW651_000751"
     CDS             9977..11395
                     /locus_tag="IW651_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0813500.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IW651_000752"
     CDS             11395..12255
                     /locus_tag="IW651_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0813501.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IW651_000753"
     CDS             12429..13202
                     /locus_tag="IW651_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0813502.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IW651_000754"
     CDS             13349..13714
                     /locus_tag="IW651_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0813503.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IW651_000755"
     CDS             complement(13862..15070)
                     /locus_tag="IW651_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0813504.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IW651_000756"
     CDS             complement(15089..15367)
                     /locus_tag="IW651_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813505.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IW651_000757"
     CDS             15560..16216
                     /locus_tag="IW651_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0813506.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IW651_000758"
     CDS             complement(16658..17455)
                     /locus_tag="IW651_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0813507.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IW651_000759"
     CDS             complement(17452..18636)
                     /locus_tag="IW651_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0813508.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IW651_000760"
     CDS             complement(18661..19908)
                     /locus_tag="IW651_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0813509.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IW651_000761"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IW651_000761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0813510.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IW651_000762"
     CDS             21888..22202
                     /locus_tag="IW651_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0813511.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IW651_000763"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IW651_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0813512.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IW651_000764"
     CDS             22887..23531
                     /locus_tag="IW651_000764"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0813513.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IW651_000765"
     CDS             23546..23875
                     /locus_tag="IW651_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813514.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IW651_000766"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IW651_000766"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0813515.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IW651_000767"
     CDS             24863..25687
                     /locus_tag="IW651_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0813516.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IW651_000768"
     CDS             25680..26027
                     /locus_tag="IW651_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0813517.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IW651_000769"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IW651_000769"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0813518.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IW651_000770"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IW651_000770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0813519.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IW651_000771"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IW651_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0813520.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IW651_000772"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IW651_000772"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0813521.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IW651_000773"
     CDS             31684..32451
                     /locus_tag="IW651_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813522.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IW651_000774"
     CDS             32441..34483
                     /locus_tag="IW651_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813523.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IW651_000775"
     CDS             34764..34913
                     /locus_tag="IW651_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0813524.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IW651_000776"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IW651_000776"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0813525.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IW651_000777"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IW651_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0813526.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IW651_000778"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IW651_000778"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0813527.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IW651_000779"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IW651_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0813528.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IW651_000780"
     CDS             39330..40412
                     /locus_tag="IW651_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0813529.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IW651_000781"
     CDS             40412..41215
                     /locus_tag="IW651_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0813530.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGVNLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IW651_000782"
     CDS             41285..42025
                     /locus_tag="IW651_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0813531.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IW651_000783"
     CDS             42036..43040
                     /locus_tag="IW651_000783"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0813532.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="IW651_000784"
     CDS             complement(43085..44782)
                     /locus_tag="IW651_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813533.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14410 a         7826 c         9341 g        13265 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcgtcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aa
//
LOCUS       DACEJR010000013        43895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEJR010000013 DACEJR010000000
VERSION     DACEJR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.013"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(229..1539)
                     /locus_tag="IW651_000785"
     CDS             complement(229..1539)
                     /locus_tag="IW651_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0813534.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1551..2969)
                     /locus_tag="IW651_000786"
     CDS             complement(1551..2969)
                     /locus_tag="IW651_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0813535.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3209..4777)
                     /locus_tag="IW651_000787"
     CDS             complement(3209..4777)
                     /locus_tag="IW651_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0813536.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4781..5191)
                     /locus_tag="IW651_000788"
     CDS             complement(4781..5191)
                     /locus_tag="IW651_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0813537.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5188..6111)
                     /locus_tag="IW651_000789"
     CDS             complement(5188..6111)
                     /locus_tag="IW651_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0813538.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6108..6986)
                     /locus_tag="IW651_000790"
     CDS             complement(6108..6986)
                     /locus_tag="IW651_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0813539.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6973..8310)
                     /locus_tag="IW651_000791"
     CDS             complement(6973..8310)
                     /locus_tag="IW651_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0813540.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8313..9416)
                     /locus_tag="IW651_000792"
     CDS             complement(8313..9416)
                     /locus_tag="IW651_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0813541.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9490..10998)
                     /locus_tag="IW651_000793"
     CDS             complement(9490..10998)
                     /locus_tag="IW651_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0813542.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10964..11656)
                     /locus_tag="IW651_000794"
     CDS             complement(10964..11656)
                     /locus_tag="IW651_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0813543.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11678..12652)
                     /locus_tag="IW651_000795"
     CDS             complement(11678..12652)
                     /locus_tag="IW651_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813544.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12952..13824
                     /locus_tag="IW651_000796"
     CDS             12952..13824
                     /locus_tag="IW651_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0813545.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14282..16480)
                     /locus_tag="IW651_000797"
     CDS             complement(14282..16480)
                     /locus_tag="IW651_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813546.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16712..17704
                     /locus_tag="IW651_000798"
     CDS             16712..17704
                     /locus_tag="IW651_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0813547.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17764..19185
                     /locus_tag="IW651_000799"
     CDS             17764..19185
                     /locus_tag="IW651_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0813548.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19178..20677
                     /locus_tag="IW651_000800"
     CDS             19178..20677
                     /locus_tag="IW651_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0813549.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20716..22110
                     /gene="uxaC"
                     /locus_tag="IW651_000801"
     CDS             20716..22110
                     /gene="uxaC"
                     /locus_tag="IW651_000801"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0813550.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22269..22952
                     /locus_tag="IW651_000802"
     CDS             22269..22952
                     /locus_tag="IW651_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0813551.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22954..23790
                     /locus_tag="IW651_000803"
     CDS             22954..23790
                     /locus_tag="IW651_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0813552.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23801..25555
                     /locus_tag="IW651_000804"
     CDS             23801..25555
                     /locus_tag="IW651_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813553.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25831..26172)
                     /locus_tag="IW651_000805"
     CDS             complement(25831..26172)
                     /locus_tag="IW651_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0813554.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26240..28411)
                     /locus_tag="IW651_000806"
     CDS             complement(26240..28411)
                     /locus_tag="IW651_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0813555.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28622..29479
                     /locus_tag="IW651_000807"
     CDS             28622..29479
                     /locus_tag="IW651_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0813556.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29550..30407)
                     /locus_tag="IW651_000808"
     CDS             complement(29550..30407)
                     /locus_tag="IW651_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0813557.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30392..33094)
                     /locus_tag="IW651_000809"
     CDS             complement(30392..33094)
                     /locus_tag="IW651_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0813558.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33107..34396)
                     /locus_tag="IW651_000810"
     CDS             complement(33107..34396)
                     /locus_tag="IW651_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0813559.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34546..35592
                     /locus_tag="IW651_000811"
     CDS             34546..35592
                     /locus_tag="IW651_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0813560.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35594..37747
                     /locus_tag="IW651_000812"
     CDS             35594..37747
                     /locus_tag="IW651_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0813561.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38220..39671)
                     /locus_tag="IW651_000813"
     CDS             complement(38220..39671)
                     /locus_tag="IW651_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0813562.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39668..41392)
                     /locus_tag="IW651_000814"
     CDS             complement(39668..41392)
                     /locus_tag="IW651_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0813563.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41385..41999)
                     /locus_tag="IW651_000815"
     CDS             complement(41385..41999)
                     /locus_tag="IW651_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0813564.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42344..43801)
                     /locus_tag="IW651_000816"
     CDS             complement(42344..43801)
                     /locus_tag="IW651_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813565.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
BASE COUNT        12871 a         8982 c         7715 g        14327 t
ORIGIN      
        1 tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg
       61 gttcttttta acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg
      121 gaattttcat aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa
      181 aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc
      241 aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca
      301 taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa
      361 gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag
      421 tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt
      481 catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc
      541 aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg
      601 aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct
      661 ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat
      721 atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg
      781 gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc
      841 atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac
      901 acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg
      961 gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc
     1021 tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc
     1081 gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc
     1141 tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc
     1201 gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc
     1261 tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat
     1321 atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa
     1381 aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc
     1441 accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt
     1501 aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca
     1561 ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct
     1621 actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta
     1681 taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa
     1741 ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc
     1801 gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt atattgatga
     1861 taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa
     1921 gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc
     1981 agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat
     2041 aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa
     2101 agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc
     2161 gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt
     2221 gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca
     2281 actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca
     2341 gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg
     2401 ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa
     2461 ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct
     2521 gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa
     2581 acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa
     2641 atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca
     2701 gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc
     2761 aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc
     2821 gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca
     2881 ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta
     2941 tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt
     3001 tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc
     3061 attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata
     3121 acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt
     3181 agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa
     3241 actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct atcccgataa
     3301 aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc
     3361 attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt
     3421 tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt
     3481 tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat
     3541 aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta aaattattag
     3601 gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca
     3661 aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc
     3721 tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct
     3781 ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat
     3841 ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat
     3901 cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt
     3961 ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc
     4021 tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa
     4081 aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat
     4141 gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca
     4201 aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat
     4261 caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg
     4321 tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat
     4381 ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg
     4441 tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt
     4501 tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata
     4561 tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat
     4621 gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag
     4681 aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc
     4741 ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc
     4801 ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa
     4861 taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg
     4921 ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa
     4981 acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag
     5041 acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca
     5101 tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca
     5161 ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat
     5221 ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt
     5281 ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat
     5341 cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt
     5401 tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt
     5461 tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg
     5521 acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca
     5581 gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg
     5641 agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga
     5701 tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc
     5761 taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga
     5821 tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc
     5881 ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt
     5941 ggataatctg ctttctatat attcatcata tcttccttct tttactatca agacctttgg
     6001 atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt
     6061 cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc
     6121 cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa
     6181 gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt
     6241 ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga
     6301 cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt
     6361 ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg
     6421 tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac
     6481 cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct
     6541 tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac
     6601 gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct
     6661 tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca
     6721 aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa
     6781 tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa
     6841 gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata
     6901 aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga
     6961 gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat
     7021 attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag
     7081 aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa
     7141 gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc
     7201 gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa
     7261 ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt
     7321 cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc
     7381 ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg
     7441 acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg
     7501 acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat
     7561 ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg
     7621 gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc
     7681 agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg
     7741 cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa
     7801 gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg
     7861 tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa
     7921 tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt
     7981 tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata
     8041 gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata
     8101 atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt
     8161 gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc
     8221 tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat
     8281 tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct
     8341 tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa
     8401 gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc
     8461 aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat
     8521 ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc
     8581 attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt
     8641 tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc
     8701 agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca
     8761 taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat
     8821 agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc
     8881 aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct
     8941 tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata
     9001 tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg
     9061 gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt
     9121 tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata
     9181 ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata
     9241 ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac
     9301 cctttaagta cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat
     9361 aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt
     9421 tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa
     9481 tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc
     9541 gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc
     9601 catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt
     9661 gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt
     9721 gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc
     9781 actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa
     9841 ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa
     9901 tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc
     9961 ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga
    10021 ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat
    10081 atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta
    10141 gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa
    10201 gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata
    10261 ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg
    10321 tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg
    10381 atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg
    10441 tttagctact tcataaagct catccatggt agttggaaca tctagtccta gattatccaa
    10501 ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta
    10561 caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt
    10621 atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac
    10681 agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc
    10741 caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc
    10801 cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg
    10861 caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact
    10921 tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac
    10981 gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt
    11041 ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc
    11101 acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt
    11161 ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat
    11221 gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat
    11281 gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca
    11341 aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga
    11401 tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga
    11461 agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa
    11521 ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc
    11581 cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg tttattttat
    11641 ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta
    11701 gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca
    11761 atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg
    11821 tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga
    11881 accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa
    11941 ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag
    12001 cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc
    12061 ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta
    12121 gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa
    12181 cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt
    12241 aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag
    12301 acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat
    12361 ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga
    12421 cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc
    12481 cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat
    12541 ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt
    12601 cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct
    12661 ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg
    12721 tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac
    12781 aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt
    12841 ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg
    12901 aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag
    12961 aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta
    13021 gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa
    13081 gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc
    13141 tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt
    13201 gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag
    13261 tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg
    13321 gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc
    13381 atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa
    13441 gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta
    13501 tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat
    13561 gctgttactt actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa
    13621 tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa
    13681 agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt
    13741 gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca
    13801 ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa
    13861 gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa
    13921 atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa
    13981 gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac
    14041 aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta
    14101 ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt
    14161 ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac
    14221 tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga
    14281 ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac
    14341 gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt
    14401 tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa
    14461 atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc
    14521 tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa
    14581 tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg
    14641 gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg
    14701 cttttacata aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc
    14761 ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt
    14821 tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc
    14881 cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt
    14941 tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta
    15001 cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt
    15061 cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt
    15121 gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc
    15181 gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg
    15241 gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt
    15301 gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac
    15361 tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata
    15421 aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg
    15481 cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac
    15541 tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc
    15601 tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa
    15661 ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc
    15721 tgtaaatcca attattataa tctgatttca gtactatata cgttttatct ttttctctta
    15781 tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat
    15841 tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt
    15901 ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg
    15961 catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct
    16021 tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct
    16081 cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta
    16141 aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt
    16201 cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt
    16261 tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag
    16321 ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat
    16381 aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca
    16441 tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt
    16501 aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc
    16561 ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat
    16621 cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa
    16681 caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc
    16741 agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa
    16801 tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag
    16861 tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca
    16921 taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga
    16981 cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct
    17041 gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga
    17101 ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc
    17161 ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat
    17221 tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa
    17281 agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc
    17341 ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt
    17401 atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc
    17461 attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt
    17521 cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt
    17581 gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat
    17641 cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt
    17701 gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt
    17761 tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt
    17821 cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat
    17881 aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga
    17941 aatatgatga accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc
    18001 gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat
    18061 gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat
    18121 acaacagagg caggaatcca attcgtggag actgataatt taacagatac tcccccacag
    18181 agcctcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc
    18241 tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt
    18301 ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa
    18361 aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca
    18421 ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc
    18481 atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct
    18541 ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg
    18601 cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta
    18661 gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa
    18721 ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa
    18781 gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt
    18841 tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag
    18901 gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa
    18961 gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat
    19021 ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa
    19081 ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc
    19141 cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac
    19201 aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac
    19261 acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa
    19321 gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg
    19381 tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa
    19441 tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt
    19501 tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga
    19561 tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca
    19621 gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc
    19681 ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc
    19741 agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac
    19801 agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt
    19861 ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac
    19921 agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa
    19981 atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc
    20041 tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg
    20101 cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga
    20161 cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc
    20221 atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac
    20281 gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa
    20341 accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc
    20401 ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata
    20461 tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat
    20521 ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt
    20581 tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg
    20641 agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag
    20701 aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa
    20761 aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat
    20821 ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc
    20881 gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga
    20941 aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga
    21001 aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg
    21061 ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct
    21121 gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat
    21181 gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag
    21241 gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca
    21301 tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca agaactgata
    21361 gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac
    21421 gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa
    21481 aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca
    21541 ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat
    21601 gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat
    21661 ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa
    21721 acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt
    21781 ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga
    21841 tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt
    21901 agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact
    21961 agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga
    22021 gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat
    22081 gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa
    22141 acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa
    22201 tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa
    22261 gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa
    22321 acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc
    22381 agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt
    22441 tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata
    22501 acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc
    22561 tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg
    22621 tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc
    22681 caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa
    22741 gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc
    22801 ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc
    22861 cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc
    22921 ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt
    22981 gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac
    23041 ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac
    23101 atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt
    23161 tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca
    23221 ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct
    23281 acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat
    23341 cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact
    23401 gttgctgttg aagcagggat caatcatcca atctataact atcagttagc taaacgttta
    23461 atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat
    23521 aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt
    23581 tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta
    23641 cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt
    23701 cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa
    23761 cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa
    23821 aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa
    23881 ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt
    23941 aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt
    24001 gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac
    24061 gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag
    24121 agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc
    24181 tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca
    24241 agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct
    24301 ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt
    24361 tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga
    24421 agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg
    24481 gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg
    24541 attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat
    24601 atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc
    24661 cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat
    24721 gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact
    24781 gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg
    24841 aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga
    24901 tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc
    24961 tggaaaatct acactcctta acttattgat tggatttatc cagccgactt caggaaaaat
    25021 ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc
    25081 cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg
    25141 cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc
    25201 actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct
    25261 gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat
    25321 cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca
    25381 agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac
    25441 gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac
    25501 ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa
    25561 aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc
    25621 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
    25681 ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca
    25741 agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt
    25801 tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt
    25861 catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa
    25921 gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc
    25981 ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg
    26041 gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt
    26101 caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta
    26161 gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg
    26221 ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt
    26281 caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg
    26341 cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc
    26401 ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta
    26461 cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt
    26521 tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac
    26581 ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt
    26641 ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag
    26701 gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat
    26761 cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg
    26821 ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg
    26881 caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa
    26941 gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat
    27001 catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt
    27061 gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat
    27121 cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg
    27181 ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag
    27241 caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac
    27301 ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg
    27361 ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta
    27421 aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt
    27481 taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat
    27541 tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt
    27601 cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg
    27661 cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag
    27721 tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt
    27781 cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt
    27841 cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat
    27901 caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc
    27961 ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt
    28021 tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt
    28081 ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc
    28141 cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg
    28201 tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga
    28261 tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc
    28321 atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt
    28381 ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa
    28441 tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat
    28501 gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt
    28561 tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact
    28621 tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct
    28681 tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt
    28741 ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac
    28801 attgttattc gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca
    28861 catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa
    28921 gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct
    28981 cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc
    29041 cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa
    29101 aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat
    29161 caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa
    29221 tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt
    29281 ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg
    29341 acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca
    29401 tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt
    29461 acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag
    29521 actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc
    29581 aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact
    29641 tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg
    29701 agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac
    29761 gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct
    29821 tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat
    29881 ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt
    29941 ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc
    30001 acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt
    30061 gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca
    30121 ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg
    30181 ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat
    30241 gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat
    30301 gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa
    30361 accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct
    30421 cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg
    30481 gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt
    30541 ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt
    30601 tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca
    30661 atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat
    30721 gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat
    30781 gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca
    30841 cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac
    30901 caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat
    30961 gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt
    31021 caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta
    31081 agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt
    31141 ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt
    31201 ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa
    31261 cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta tctactaata
    31321 cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt
    31381 taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact
    31441 ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta
    31501 gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt
    31561 tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg
    31621 gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat
    31681 cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg
    31741 tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta
    31801 ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat
    31861 ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat
    31921 cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg
    31981 ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac
    32041 tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga tcatggggat
    32101 agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc
    32161 gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag
    32221 tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg
    32281 gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat
    32341 ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat
    32401 gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta
    32461 atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc
    32521 tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca
    32581 tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg
    32641 gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg
    32701 tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt
    32761 tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa
    32821 aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt
    32881 ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc
    32941 catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta
    33001 tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt
    33061 ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac ttttctactc
    33121 taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt
    33181 ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg
    33241 cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca
    33301 ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa
    33361 ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc
    33421 ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca
    33481 gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg
    33541 cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga
    33601 tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa
    33661 aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt accagatatc
    33721 cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg
    33781 tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa
    33841 atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga
    33901 taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat
    33961 aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat
    34021 cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat
    34081 tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt
    34141 cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt
    34201 ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct
    34261 ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg
    34321 catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta
    34381 tatctttata agccactaat aaaaccttct ttctatttga tatatcttta aagattatac
    34441 ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc
    34501 attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg
    34561 taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac
    34621 agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc
    34681 aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc
    34741 agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg
    34801 attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg
    34861 aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa
    34921 aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat
    34981 acagaacaac atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata
    35041 ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc
    35101 ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt
    35161 ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg
    35221 gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa
    35281 ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc
    35341 gctatccaga ccatgaatct cttgagacag catggttatc atatccctga agatttttca
    35401 attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc
    35461 gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag
    35521 acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca
    35581 aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc
    35641 aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt
    35701 tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt
    35761 tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct
    35821 gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta
    35881 ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg
    35941 catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa
    36001 aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc
    36061 ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt
    36121 ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa
    36181 aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg
    36241 gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag
    36301 caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca
    36361 gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt
    36421 tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac
    36481 gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta
    36541 taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga
    36601 aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga
    36661 acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga
    36721 tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca
    36781 aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt
    36841 tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg
    36901 tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca
    36961 atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa
    37021 agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg
    37081 aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata
    37141 tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt
    37201 tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt
    37261 tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat
    37321 aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga
    37381 ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt
    37441 caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc
    37501 attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac
    37561 gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac
    37621 gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg
    37681 gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta
    37741 taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa
    37801 aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa
    37861 caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg
    37921 gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    37981 acaccgttta gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt
    38041 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt
    38101 tgaactccgt ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc
    38161 ccaaaattag atttctagac aattttttat aagttatttc attacattgg ctttttcttt
    38221 tatgattctc cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat
    38281 aatttagaga aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt
    38341 tgattactgt gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct
    38401 gaaaaacttt ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc
    38461 aggttttctg acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg
    38521 attacttttt ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac
    38581 agaagtagct gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt
    38641 aaatattcct catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa
    38701 ctggaatatc gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca
    38761 gtgatcattt tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa
    38821 aaatcgatgg tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta
    38881 tctccataaa attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt
    38941 ttgtccaaag aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt
    39001 tcttgaacaa aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat
    39061 acacggtctc cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta
    39121 ttgataagag tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca
    39181 cttatttgtc ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga
    39241 ctgaataaca gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac
    39301 cgtgttttga ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa
    39361 taattcacgg cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat
    39421 aaaatcataa attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca
    39481 tttatttctg gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca
    39541 gctagtcctt ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa
    39601 tcaattatct tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga
    39661 tacacgctca tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct
    39721 caagcgacat ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca
    39781 gaccgataga accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta
    39841 gccgtttttc agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt
    39901 cctgcaagac tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa
    39961 aataattttc aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag
    40021 gatcgatcat aaaatgatag gctactcgat taggataacg catttgatac aaatagacat
    40081 atttttcgca aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt
    40141 ttcgaagcaa agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga
    40201 ttgcatacat tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt
    40261 gaagcgcccg catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa
    40321 actgattgat gctatccaac atttcgttga taccgattga aagttccttc agttcatatt
    40381 ccgtgtttgt tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct
    40441 gcatgacgat agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca
    40501 gtatattgtc taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga
    40561 tttttacact ttttttatct agtacagcta atatacgata atcatcagac agttcttggt
    40621 gttgaacaaa attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac
    40681 tctctgtgat gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga
    40741 atgtgtatag tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg
    40801 ttagattttc taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg
    40861 actcagctcc tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg
    40921 tccccttttt tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt
    40981 gattcaacac gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt
    41041 gatttgggaa agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat
    41101 actgatctat gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta
    41161 caagctggtt tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc
    41221 tttgaacggc atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga
    41281 tactaaaaat aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca
    41341 acagacgatt tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc
    41401 aactgtttat ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa
    41461 agaccaatga caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata
    41521 ccgctaccaa taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt
    41581 agctttagca gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga
    41641 taagtagcaa ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg
    41701 agccctttga tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa
    41761 aataaactaa atagcagatt gcctcttaaa aaatttcttt tatatatatt ccagcctctt
    41821 tttattgtga tttctttata ctcgaatccg tacaaataaa atagttcatt gactgttttc
    41881 catgccgcac ctaatccaaa gatgatcccg ccggcaaatg tatatatcca gaaatacaag
    41941 gttagtttta taatgaccca gatccggata aataactctt ccaaaacctt ccctaacata
    42001 tcttctcctc caaacattaa gtttatactt tctcattata caaaaaaagg ttgggcaatc
    42061 agcaccttga cctgattatt agagaaacaa acggaaaaat agttcctcat attttgtcgt
    42121 tttgtgagcg aataccgagg gttagctgat tgaacaccgt ttaccaagag tttgtgaaat
    42181 agccgcttag tttcgagtag taagaaaaat tttgtgaata tggctttcca catttttaca
    42241 tatatttagc ttaataccga gaaactggct tttgaacacc gtttattcgg aacaagaggt
    42301 tgtgacacgt cttttgtcac aacccctttg tctttattca ttattattct tgtcggaact
    42361 catcatattg tttttgcatt tctttcaata ccttatcata gccagccttg tccaaagcgt
    42421 ctttcaattt cggtaatgtt tcatctggat caacagtccc tgtattcaat ccatctaaat
    42481 attgattcat gacattagaa atattactta attctgtttt gacactgtca gtgttgaagc
    42541 tgaatcctaa aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt ttggcaatca
    42601 tgtcatccgt aatagactct tgtgtatata gaatcttgtt gtttcctgta ttccaagcag
    42661 acatatgtgt atttggctga taaccatcta gcaatttgat tttatcatct gaaccatcga
    42721 ctttttccca tgcttctccc tcgatccccc aaacaagccc attcaataat tctggatcac
    42781 tgttaagcag ccctagaagt tctactgatt tttctttatt ttttgaagta tttgatacga
    42841 caaaatttgc catttgtgct tgagctgtag attttagtgg aacagtaatt gcttttgaga
    42901 caagttcttg ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg
    42961 tttctccacg cattagccaa gtatttcctt ctagtggata atccttattg ctcgttgcag
    43021 cgtcacttgg aatgagtcct tgtttgtacc agctatgcat tgtacgtaac agtccgatat
    43081 acgtttcatt atcgtattgg ttgatgattt tcgaatcatc tccatccaga tcaatagcaa
    43141 atggtaacgt attccctaaa ggataatcaa agttcccttg gactttgaag ccttgtccaa
    43201 tggcaaaagc tgccgtgttt ggttcttttt catggaattc cttcaagact ttttctgcat
    43261 cagcgtaaga ttggattcca tcaattgaaa gatcgtattt atctagtaaa gctttgttaa
    43321 aagtaagtac ttgttgagca aagacatttc cattgactgg gaaagcatat aattttccat
    43381 tgaccagatt tcctttaata tatgcttcat ctaaatcatt ataagctttt tcagcgtact
    43441 taggcgctaa ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa acatagttgt
    43501 tcgcaaaggc gatatcataa ttttccccgg aagaaatgat gacgttcatt ttcttttcgt
    43561 aatcgcccca gccaatgtaa ttgatattga cttttacacc gatctctttt tcgattcgtt
    43621 tgtttgctac ttccatcaat tgatcgaaat tatctggttt atctccgatt tgatacatct
    43681 gcaaagtgtc cgaattgcta gcttctgcct ggtctttaga accagaacct ccgcatgctg
    43741 ctaatgttcc caacaacaac gctgaaacac ctgtaaaggc tagtttcttc cataacttca
    43801 tttttcttcc tcctaatttt atgatttata tttttgtttc tttattcttt tactccgccg
    43861 attgttagac ctttgacaaa atgcttctgg aaaaa
//
LOCUS       DACEJR010000014        40936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEJR010000014 DACEJR010000000
VERSION     DACEJR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.014"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1190)
                     /locus_tag="IW651_000817"
     CDS             complement(39..1190)
                     /locus_tag="IW651_000817"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0813566.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1203..1640)
                     /locus_tag="IW651_000818"
     CDS             complement(1203..1640)
                     /locus_tag="IW651_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0813567.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1655..3421)
                     /locus_tag="IW651_000819"
     CDS             complement(1655..3421)
                     /locus_tag="IW651_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0813568.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3685..4362
                     /locus_tag="IW651_000820"
     CDS             3685..4362
                     /locus_tag="IW651_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813569.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4365..5492
                     /locus_tag="IW651_000821"
     CDS             4365..5492
                     /locus_tag="IW651_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813570.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5692..6132)
                     /locus_tag="IW651_000822"
     CDS             complement(5692..6132)
                     /locus_tag="IW651_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0813571.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6143..8176)
                     /locus_tag="IW651_000823"
     CDS             complement(6143..8176)
                     /locus_tag="IW651_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0813572.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8178..9881)
                     /locus_tag="IW651_000824"
     CDS             complement(8178..9881)
                     /locus_tag="IW651_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0813573.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9975..10925)
                     /gene="manA"
                     /locus_tag="IW651_000825"
     CDS             complement(9975..10925)
                     /gene="manA"
                     /locus_tag="IW651_000825"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0813574.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10964..11989)
                     /locus_tag="IW651_000826"
     CDS             complement(10964..11989)
                     /locus_tag="IW651_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0813575.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12198..13067)
                     /locus_tag="IW651_000827"
     CDS             complement(12198..13067)
                     /locus_tag="IW651_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0813576.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13526..15418)
                     /locus_tag="IW651_000828"
     CDS             complement(13526..15418)
                     /locus_tag="IW651_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813577.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15415..17142)
                     /locus_tag="IW651_000829"
     CDS             complement(15415..17142)
                     /locus_tag="IW651_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813578.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17158..17808)
                     /locus_tag="IW651_000830"
     CDS             complement(17158..17808)
                     /locus_tag="IW651_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0813579.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18012..18308
                     /locus_tag="IW651_000831"
                     /pseudo
     CDS             18012..18308
                     /locus_tag="IW651_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18480..19100
                     /locus_tag="IW651_000832"
     CDS             18480..19100
                     /locus_tag="IW651_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0813580.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19192..20358)
                     /locus_tag="IW651_000833"
     CDS             complement(19192..20358)
                     /locus_tag="IW651_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0813581.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20370..20723)
                     /locus_tag="IW651_000834"
     CDS             complement(20370..20723)
                     /locus_tag="IW651_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0813582.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20851..21873
                     /locus_tag="IW651_000835"
     CDS             20851..21873
                     /locus_tag="IW651_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0813583.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22312..22746)
                     /locus_tag="IW651_000836"
     CDS             complement(22312..22746)
                     /locus_tag="IW651_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813584.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22856..24715)
                     /locus_tag="IW651_000837"
     CDS             complement(22856..24715)
                     /locus_tag="IW651_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0813585.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24833..25993)
                     /locus_tag="IW651_000838"
     CDS             complement(24833..25993)
                     /locus_tag="IW651_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0813586.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26300..27724)
                     /gene="araA"
                     /locus_tag="IW651_000839"
     CDS             complement(26300..27724)
                     /gene="araA"
                     /locus_tag="IW651_000839"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0813587.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27740..28438)
                     /locus_tag="IW651_000840"
     CDS             complement(27740..28438)
                     /locus_tag="IW651_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0813588.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28395..30056)
                     /locus_tag="IW651_000841"
     CDS             complement(28395..30056)
                     /locus_tag="IW651_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0813589.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30274..31674
                     /locus_tag="IW651_000842"
     CDS             30274..31674
                     /locus_tag="IW651_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0813590.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31926..33005)
                     /locus_tag="IW651_000843"
     CDS             complement(31926..33005)
                     /locus_tag="IW651_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0813591.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33091..34050)
                     /locus_tag="IW651_000844"
     CDS             complement(33091..34050)
                     /locus_tag="IW651_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0813592.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34062..35585)
                     /locus_tag="IW651_000845"
     CDS             complement(34062..35585)
                     /locus_tag="IW651_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0813593.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35595..36158)
                     /locus_tag="IW651_000846"
     CDS             complement(35595..36158)
                     /locus_tag="IW651_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0813594.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36164..37027)
                     /locus_tag="IW651_000847"
     CDS             complement(36164..37027)
                     /locus_tag="IW651_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0813595.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37041..37937)
                     /locus_tag="IW651_000848"
     CDS             complement(37041..37937)
                     /locus_tag="IW651_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813596.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38112..39383)
                     /locus_tag="IW651_000849"
     CDS             complement(38112..39383)
                     /locus_tag="IW651_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813597.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39373..40890)
                     /locus_tag="IW651_000850"
     CDS             complement(39373..40890)
                     /locus_tag="IW651_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0813598.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11796 a         8583 c         7092 g        13465 t
ORIGIN      
        1 caaaaatttc gtcacattcc cctttttcgt atcctatttt aagcgacttg cttggcataa
       61 cggttcacgt tttgcttgat attcccgatt gttttcggat cattgattcc tgtgacttct
      121 tgaatcaatt gttccaagtt ttcatggatc ttcatttcat gcaattgacg gctttgttca
      181 tcttcaggat catagtaatt aaaaataatc cccattgttg ccgtcaaatg tgggcaagtc
      241 aagttatgtt cttgtagttc tctcactgga cgtgtaaatc tttcttgata acctaactta
      301 cgtaatggtg tccgagcgac acgtgtgatc gcatcagaga tatttttgtt ttggaagcga
      361 tgaatgattt tttcaatata cgcatgatgc tgctctgcat caaaccccca tttggcaatc
      421 agcaaacttc ctgtttcctg caaaactgat tttaattgtg ccacaactaa cgcatcttgc
      481 attgcttcat caatggtctg gtaacctaac aaggcacctg tataagcgac agtcgcatga
      541 ccagtattga cactaaatag tttgcgttcg atatatggtt ctagatcatc tacatataaa
      601 acccctttta attggatttt tttatttttt ctttggctat cgtcaatgac ccattctttg
      661 aaaggttcca cttggacgaa taaaggatct tcatggtgtt gtaatgggac gattcgatcg
      721 actgctgcat ttggaaagcc gatatatttg tcagtaaatg atgtttccgg aagatattta
      781 tacacttctt tttctagaaa tgctgaaccg ccaatcatat tttcacaggc aatgatatct
      841 aaagatgttg tgcttccttc cttttctctt tcttggatac cttttgcaat caactcagca
      901 ataaaaggta agatattcgg gccaatggcg gtagtaacta aatctgcttc agcgatttct
      961 tggattactt tttctggatt tgtttgattg ttgattcctt tgacattttt tacagtgatt
     1021 ttttcttttg attcatctgc taattcgatt tcgtattctt ttctttcatt taaagcatca
     1081 atgatcgtgt gattcacatc tacgaaagta atctcaaatc cattagctgc caaaacctct
     1141 ccaataaatc ctcgaccgat atttcctgca ccaaaatgta ctgctctcat ttttctctct
     1201 ccttaagcaa ttgctagatt ttccgttact tcttctttgc tgagcgcgtc tgctagctgg
     1261 acaacattgt ccatatcact gcaataaagt gcgatttgct gtacaagttg caaatgctca
     1321 tctccgactc ctgcaatacc gaacaaaaca gtcgcaatct tttcttcttc ttcggtacca
     1381 aaatctacac catctggtac ttgaacgaca cagatgccgg attttttcac atattttttt
     1441 gcttcatctg ttccatgagg aatcgcgata aaattcccca tataaacaga taacattttg
     1501 tctctatcta ccatggcatc gatatattcc tcatcaacgc accctgcttc tacaagcatc
     1561 tgaccgcaat agcggatcgc ttcttctttg gttttaaaag atcgattgag ctcgatcatg
     1621 tcgaatggca aactttgcat gctgttttcc tcccttaagc tgtctgtttc aaatattgtg
     1681 caatcgattc gtatttttgc ggattgacca tgctgttgac tgataaatga atggattgag
     1741 gtgcagccat ctgtactttt tccgctaaac tttctttggc tacgatcagt gaatcaggtt
     1801 catcttttaa atcttggata ttgacttttc ttaacgggat agttaaatgg ttcttgtcta
     1861 atatatcttt gagcacggtc atccccattg ttgctgatcc ggaacgatca tcgtggacaa
     1921 gtagaatctc tttgatttgt ttgatgtttg ctgaaagaaa ctcttctgct ggctcattct
     1981 caatttcaga aactgatgtt ttctgcttgc tgcttaattc ttgaatgatt tcatcatatc
     2041 gaggtgaatt caaaaagttt tctactgaga caaaacgcgc gctcggtgct ttttgttttg
     2101 ctctttcttg tagttcgacc tgagtaacga tcaatgtatt cgagtcatct gtcagttggc
     2161 tgattgcttg attggtcacc gtttgaggca agtctgcttc ctgtactttt tttctcaaga
     2221 tcgaagcgcc catcgcactt gatcccattc ctgcatcaca agcaaaaatg attttttgta
     2281 cgccagagaa tgtagctcct tgaggaatac cttgggcacc tttggaagct tgcttagctg
     2341 cctttgtctc ttgaacagat ttttcgaagt cttcatttgt atctttcgca tccgctttca
     2401 aaattactgc agaaatacca aacgaaacag ctgcggcaac aatcacgccc aaaataacag
     2461 gaaggtaaga attgaacggt gtcatcgcta gaatcgcaat gatcgaccct ggagaagctg
     2521 gcgctcttag tccagcatcc atcaattgga aaatgaaact tcctgacatt cctccgccta
     2581 taactgataa gaataataac ggtttcatca taacgtaagg aaaatagatt tcatggatcc
     2641 cgcctaaaaa gtgaatgatc atggctcctg gtgctgaaga ttttgctgct cctttgccaa
     2701 acaaagtaaa agcaagtaat acgccaagtc ctggaccagg atttgcttca agcaagaata
     2761 gaatagattt tccagcctct gctacttgtt cagttcccaa tggtgtcaaa atcccatgat
     2821 tgattgcatt attcaaaaat aagatttttg ctggttcaat aaaaatactt gtcaatggaa
     2881 tcaaatgggc agataaaatg atctctacac cttttgccat tgcttgagtc aatgcatcga
     2941 caactggacc aatcgcagaa aaacctaata acgctaatcc aaaaccgata agacctgctg
     3001 aaaaattatt gactagcatt tcaaaaccgg atttgatccg tttttggaat agctgatcaa
     3061 atttcttgat ggcatatcca ccaagaggtc ccatgatcat cgcgcctagc atcattggaa
     3121 tatctgttcc gacaatcaca cccatcgttg caattgcccc aacaactgat ccgcggtcac
     3181 cagcaattac ttttccgcca gtataaccga tcaataaagg aatcagataa gttagcattg
     3241 ggcttaccat ggtagccaaa gcctcatttg gtaaataacc atctgggata aatagagccg
     3301 ttaatacacc ccaggcaatc agtgctccaa tatttggcat gaccatgctt gataaagtac
     3361 tgcctaattt ttgtaccctc gcttttagcg aaatgttgtt tttgggaatc gtccgttcca
     3421 ttttgtctct ccctctctcc ttttgatact ctaattgtat ccgcttacta aaaagagaaa
     3481 tagtcgtact ttggcacgaa actttgtgcc aaaggtttga gtatgtcagc aaaataaaaa
     3541 gatttcggta tcagaaaact tcaattttaa ataaaactgt tattttaatt tttttataat
     3601 atgcaaacaa taacttttgt tagcatttaa tttagatgtt ttccattcta ttatgttatt
     3661 aaattttata ttggaggcct atctatgaca ttactgatca aaaacatgaa catgaaacgt
     3721 ggtaaaaaac gtgtgattcg acaattgaat cttttagtag aatcaggaga agtagctgct
     3781 ttgattgccc cgaacggatt tggcaaaaca actttgctga atggcattgc ccaactgctg
     3841 ccaacaactt atgatcaatt gtcccttcac aacatctctc ctactgctta tacagattat
     3901 cgccggtctt tcttttttct agaatcaacg aatcagttat ccgactatct atcggctgtt
     3961 gattatttga ctttgatcaa atactattgg cagagcaaag tagaatgcca aactgttttc
     4021 caacgattga agatgactga gtaccttact ttgccgattg gaaaaatgtc tctaggaatg
     4081 aaacaacatg tcttgcttgc tgcttacttg atcagtgatt catctgtcat gttattcgat
     4141 gaaccgttga acggattaga cccaggaagt attgatatct tgaatagcct catcagacaa
     4201 tggcagaaag aggggaagac gattcttctt tcttcccacg ctatcgcgaa tatccaatcg
     4261 atttgttcta aagcatgttt tctaaaaaat ggagaaatcg tcacagaatc ctcaaatatg
     4321 gaagagattt tagctattta tcgtaaatta tatgaggtgt aactatgtct ttacttacat
     4381 ttgaacgcaa tctcttacta aaaaacaaag taaacttttt atttcctcta ttgttggtcg
     4441 tgctctttgc attcccactt ttttttgatc ataaacttgc ttatacagaa ttcgatgaac
     4501 tgaatcataa ctatgaagag atgcaacgtt tgattgaaac gttgaaagaa gatgagaatg
     4561 agaaagaatt tgtggaaagt ttagagaaaa gcaacaaact gatcgaagca atcttacatg
     4621 caaaaaacac agggaatgtc cagcaaatgg tcgaagcaac ttatcattat gaaaaagata
     4681 tactggatcg actgatttct ggtcaaagac aaggaattcc aatcattgaa caacaaaaac
     4741 gtgtcgagtt gctccgttat atgaaagaac atcagatcca aagatattct atttttgact
     4801 tgccagcgca tctcccttta gcaaattact acgaaaatat tttttcagga atgatttcta
     4861 gttttttgat tttatgtata accgccttgt ttctttcttc catcatctct tatgaaaaaa
     4921 gaaagcaggt aatctctcta gtcaatctat taccggatag tatggtaaag aaacatagca
     4981 tccgttttac catttattat ggtgcagcta tgctaagtct cgtgatgcca tttttgatcg
     5041 tctctatact agtaataatc aaaaatggtt tgggagactt ccgttatcct gtaggaacga
     5101 tcattggtca ggagatacga atccttccga tgtatgagta tcttttccag agctttctgt
     5161 tcctgttgtt gtgggtgctc tttttaagta caatcagttt tcttctatct gctttatttg
     5221 aacattctct agtcaatctt ttaggtacac ttctttgtct tttcttagca gagtatcgct
     5281 tgttttcttc gattggatgg atagaatcaa tcagtcatta cttgccaact tcttatgttg
     5341 attttcaaaa tgtgatcata ggaggagata ttttctcgcc actagcttca gaacaggtta
     5401 ctttcatgaa tggtatcttg acacttggta tatggagtat cgttttatta ttcataggta
     5461 tgggcactat ctatataaaa aagagctatt aaaacacaaa aataataggg gcggtgacaa
     5521 aaaccttgtc accgtcccct atttcaaata aacggttttc tagaataaat agttttcaaa
     5581 agcttgcatc cgcagtaata agaacctccc aacaaaaata gctcctctta tttttatctt
     5641 ggttgaagtt attacttgat gcaaggcagc tttttttact gtgaacattg gttatatatg
     5701 tccatgttca aaaatcattt ggacttcttc aatggttttc gcatcactga gcgccattac
     5761 ttggtcgatg tctgaacaga aaaaggctaa atcttggagc accgctaact gatgttcttt
     5821 ttttaaagcc actacaaata agagtgtcgc gatttttggt tcctcttttg ttccaaagtc
     5881 cacaccatca ggcacttgga ctaaatagat tccttcttct actacttctt catctttgtc
     5941 agtcccatgt ggcaaggcta cgaaattccc gatatagaca ctcgcttttt cttgtctttc
     6001 gatcattgat tggatatatg ccggtttgat tcccccttgt tgggtcatcc actggccgat
     6061 ctcggtcaac gcttctttcc aattttgata ggattgatcg agcaatattt tttctgctgg
     6121 tagctgtatc atcttttcac cttcatttct ttatttcttc aatcaggatt ttcgctaaca
     6181 actgataaac gatttcttta tttcctgagc ggaagatctc ggtgttcaag tcattcatga
     6241 tgattgctcc actgatcttt cctaaaagaa tcccgtcttt ttcttggatt ggcgaaggcg
     6301 ccagcataac taacatacgg gacaaatcga ctggctgtct atccatccct tcaatggtca
     6361 acggatgttt caaattgaaa atacaaaaga tcggtttaga aatcaatgag ctggatgtat
     6421 ggaataaccc catatttgta tgaggaatac cgataggtgc ttgttcatat cgcttaagca
     6481 gccggcgatg aaccacctct tcatccgtta ctagtccttc aggaaaataa ggaaataagc
     6541 tgcgaatcgt ttcggaaatc gtctcaggat tatccagttc ttggataaaa aaacgaccga
     6601 gtaattgatt gatttgatcc atgaaatgca ttgtttcctc ataagtatct tctatcatcc
     6661 actcgttatt gctgttatac gtaatttttt gccttttttt cttctcaata tgcgaaaaag
     6721 cttcttttag ctgcttgact tcatcttcta gcagtagcgg ggagactaaa atatattttc
     6781 caccgtatcc tggtaaaata gatgtagaaa taacaacatc atattcttct tgtaaatcga
     6841 ttttttttaa atcagcaatc cgatagaaat gaatttgttg gatgaaggga aaataccttt
     6901 tcaatcgtag ttccaacatg ctagtagaag ctaatccact tggcgaaaat cctgcaatac
     6961 tgatattgct ttctttcgga cttcgttcca atgaattcgc aaaatgaagg accatataag
     7021 cgatctcttc ttcggataat tttttttctt gaaaaatccg ctctactgct ttacggattg
     7081 cattcgcaac ttcctcgtat tgaaccatga ttttttctaa aatcggattg gtcagtgtct
     7141 cttcttgtaa aacggctctt gaaaacacac cagataaatg agtcaacagc attttgtaca
     7201 aagtttcatc ttcataaaaa tggacattcg tctcttcact tacttcttga atcagttgtt
     7261 ttactaaata agcaagctgt gtatcgaacg tctcttcaaa gaaattctga tcaaatgagt
     7321 tcgtgaagtc atttaataat cctgcaaaaa agacgatctc atttactgga taaagttgtt
     7381 ttgtctcaga agcgatccgt gaaaacaatc gtttagctgt ctgtagtaat tctttgtttg
     7441 ccagactatt atatgtctca tttgtcagta aatgattttt agtcacacgc tccatcgtca
     7501 atgtcagcat caaaaccaaa tgttcaagct ttctatcact tagattggtc tttttttgga
     7561 tcaattcttc cccgattact ttgcgtgcaa gagatagatg ttgcggatca acgaatcgaa
     7621 aaaaataatt cagtgatgca tcgaaagaaa tactatgaaa aagttcatat tcattgattt
     7681 ctaattctaa tacattcgct gtcagaagcc ggcggtattt ttccggtcct tcgatctcgt
     7741 atccctggtt ccgaatgatt tccaaaggca gttggcgaat actttcttcc aactgtttga
     7801 tatctgcata aaaagtggta ttactgatgc gatatgattc catgaaatga gcaagactca
     7861 acggctctgt cgtcagtaac aactcggcta aaatcgcgtg ttgtcgttta gcagtggata
     7921 agtcattact tttcccatct gtattttctt ttaattgctc taatacttct tctgatgcct
     7981 caatgacaaa tgagcctcta gctgcttttt tcaaagaagc atttaccgat ttcaatgaga
     8041 cttcaagatt atctaattcg cggtaaatag tccgtttact gaccttcaat aaattcatca
     8101 ttttttggat agatacaggt gacgggctac tgagaaattc tgctaataat tgtttttctc
     8161 tgggcgataa atacatctta tatttctccc ttcacgagtt tttcgtattc attcgtagct
     8221 acccaatgtt caactagaac tagccgaggg atcttttctt ctagctgttc agttacagct
     8281 agttctcttt gtgcgatata aatcgtttct tcttggattt tgattttttc tattgattct
     8341 tttgagataa caaactgttt tttcgctttt tttgctaatt gacttaaaat cgtcatgccc
     8401 atagtttgtg agccgcgtct gttgttttta tacaaaaata caatattagc aggcgacttt
     8461 ttttgttctg tgatatctgc tatctctttt ttttcttccc gctttggaaa ttggatcgct
     8521 tcttttattc gtgacagcaa gatatctgtt tccaaaaagt gatccagtac atagatttga
     8581 ctttgaggta ctttttctct tgcaagaccc gctagttcat gttgcacgat aaccagctga
     8641 tttacttggt tattcaattc atagatagag ctgtatccga cagaaagatc gattttttct
     8701 gctgctaatt tctttttcag cagggcggct cccatagcac ttgatcccat acctgagtcg
     8761 caagcaaaaa tgacggaatg aatgatagat ggttccaaga tttctcccct ttctttgacc
     8821 gttttgttct ctttttcttt atttggtaat ttcaatagta tcgctgcaca aatgaaggtc
     8881 acaaatgtac tgattgctat ccctatactt accccagcta gttgtggata aggcgcattg
     8941 gcaaaaatca cgagtagtga cccaggcgat acaggtgcag ctaatcccgt atctaaccat
     9001 tgaaatatca acgtgccgct tgccccacca gccattacag ctaaaaatag atacggtcgc
     9061 agcaagacaa atggaaaata gatttcgtga atcccgccaa tcgcatggat catcaatgct
     9121 ccactcgcat ttactttttc ctgtttttct gcaaaacaaa ggatagaaag taagatccct
     9181 aaaccaggtc caggatttgc ctccatcaaa aataaggaag agtacccgac tgtttgtgag
     9241 aattcgattc ctaaaggtgt caatacacca tgattgattg cattattgaa aaatagtact
     9301 tttagaggtt ctaagatcag actgacaagt ggtaacagat tcattgtgac tagccaagac
     9361 acaacggagg cgagcaacaa gtgaacacta tttaaaactg gttcaataaa aacgattcca
     9421 attatacaaa taacgctacc agtcagtcct actagtaggt tgctgacaag catttcatat
     9481 ccagccttga atttcacgtg gaatctagaa acaaattgtt catatacgat gccggcaagc
     9541 ggcccgataa tcatcgctcc tacgatttgt ggagcattgg aacgagcgat caaccctaaa
     9601 gaagcaattg caccggcgac tgcccctttt tgtcccccta ccgatttccc gcctgaataa
     9661 ctgatcaaga tgggtaaaag cagctggatc atccaatggc atacctcttc taatgtggtg
     9721 tgcaaatcgc cagacacacc ttttgttgct aatgacaaga ttccccaagc gataaacaga
     9781 cttagatttg gcatgatcat ttcatttaag tgatgtccag cttttctcag ttgtttcata
     9841 gacacgcgac tgctgttgtt tgctttccat ttgtattcca tctttttcac catacttcta
     9901 tttattgatg gacggatttt atgtagaaaa agcagcaaac gaaaaaccgt ctgctgcttt
     9961 cagctatctg ggaactattt gcctggttca gtcgcaatga ttgctagttc accttggacc
    10021 gcccactcag aaacgccatt tggaataata cagcttgttc ctttttctaa tggatatgtt
    10081 tttccgtcaa cgacaaggtc tcctgctcct tcgattaccg tcatcaatgt ataaggggct
    10141 tgttttttga aagatgtgat tcctttaata tcccactcat atacattgaa aaactcagtt
    10201 tctaaataag tgacgatcga tgaattccct tttcggactt ctttgatttg taattctggt
    10261 gttttcgcag ggacagttgt tacatcgatc gattgttgga tatgcagctc tcttgtttgt
    10321 ccttgcgcat cttttctgtc gtagtcatac acgcggtaag tcgtgtcaga actttgctgt
    10381 gtttctaaaa tcatgattcc tttaccaata gcatggatcg ttccgcttgg tacataaaag
    10441 aaatcacctt ttttaactgg tactttcttc aaaaggtcat cccagcgccc atctttgatc
    10501 atctcagcta gttcttctct tgtctttgca tgatgaccat agatgatttc tgcaccaggt
    10561 tcagcatcga tgatatacca gcattcagtt tttcctaatt caccttcatg tttcatccca
    10621 taggcatcat ctggatgtac ttgtacagat agatcatctt ctgcgtctaa gatcttgatc
    10681 aataatggaa acactggttc tgaaggatgg ccaaacaatt ccttgtgttc tgaccatagt
    10741 tcatctaatc ttttcccttt gaattcgcca ttttctacta cactgacacc atgaggatgc
    10801 gcactgatcg cccaatcttc tccgatttta tcgttaggga tatcaaaacc aaagactgaa
    10861 cgtaaacgac ttcctcccca aattttttct tgaaaaactg gttttaaaaa cataggttcc
    10921 tgcataattc aaactcctct catttttcat agctattata gcattatttc ggtaataccg
    10981 ctttctcttt caaaaagttt tcaacaattt catctcgaca tatgagatac tccgcacgat
    11041 gatcgacagg atgtacgcga tttgacaaaa atatcaacgc ttcttgtgtt tttatatcaa
    11101 tcaagaaaaa cgtacctgta taaccagtat gaaaaagaat cgcatgaccg tcttctacat
    11161 ccttttttaa gtcccatcct aaagaacgat agccattttt tttcggtgtt tgatcttgca
    11221 gtaaagaatc aatcgtctct tcttctaaaa aacgaccatt acggattttt cccttgttca
    11281 aatacatttg gacaaaagta atgctgtctt tcaaatcaat aaagagaccc gcgttacctg
    11341 catgttcagc caaaactctt gctttcggat catgagtttg tccacgcaaa acctcacccg
    11401 aaggcagttg ttctgtggga acgatttttt tggaaggaaa aggaagaaat aagctgttca
    11461 tcattcctaa tggttccaat acttgttctt gaaagatttc cactgcagtt tcttggtaaa
    11521 tttcctctag catcaaccca agaagaatcg taccggcatc tgtatatttt acttcttttc
    11581 ctagttgctc acctggttct agcttcaaat aagcggcgat caattcttcc tgagatagct
    11641 tatctctgtt tggtatccat gttttgatat cagaagtatg tgtcagtaaa tgacgcaaag
    11701 tgattgtctg attttcaaat tcaggaagat atttatgaag cggttgatcg agttctatct
    11761 ttccatcttc taatagacgt aaaactaatg aagtggttgc aacgactttc gtcagtgagg
    11821 ctgcatcaaa caacagatct tctgtcatca gctgcttttc aggaacgact tgggcaaagc
    11881 cggctgtaaa tgtttctact tgattttctt ttatgaaggc gactgctaca cctggataaa
    11941 atccttcttc catctttttc aaaataagct gtttcgtgtg tggatacatc tttttctcct
    12001 gcttttctat cagattctta cagtccaagc ttaaagctta atctatgagg aatcaacctt
    12061 ttttacacaa aaaaactgcg cgaaaaaccg cgcagtttcc cccggtatac tattttctct
    12121 ttctatctct agaataagcg atagtatgac cgttcataaa atgacgagac atgcgacaat
    12181 cgttaagaaa gaaaaagtta agcgtcgcat cgaatgtcag ttttattctt ttcttttggc
    12241 tcactgacga accaccattc gattcctata gcatcataga ttgtgagtac tgtgtgcttt
    12301 ttgtctaaga agaaatcaac tgatagttgc tgcaagtgag caatcagtcc ttccatgctt
    12361 tcagtatcga tcatgaattt cagaaaatca agtccaatca cttcatctgt aggcaaatca
    12421 attgcgcctc cgctagcttc tgtcaaacca acatggaaat ctccttcatt caatacatgg
    12481 atgccctctt cttcattatg taccttcaaa ccaagtacat gattaagaaa ttggcgttct
    12541 tttgttaagt tagtaacatt taaatggatt ttttcaaaat aagaacctgt tgatagttta
    12601 ctgaattttt cttcagattt tcttaataat gcttcttgat ccattgtttc tggaatttct
    12661 ctttggtcgt tcttattctc ttcatggtaa atttccaatt cattctcttc tgggtctacg
    12721 agcaaaatcc ccatacgtcc atgatcttct aaagcttctt tgattggata attcatttta
    12781 cggatacgac aaaggatatc tgccatttct tctgtgctag ggataatcaa tgaaaaacgg
    12841 ttcagctttt tgatcacccc catgtgatca ttcgctctag gactttcttc cagcagcaac
    12901 atttcacttc cagcttcttc tgttcctaaa atggctaatt cgttttcttc tcttttcaaa
    12961 tcaaagccaa caacgttctt gtaaaaatca atcattttat ctcggtcttt cacccgaatg
    13021 gctacacttt ttaaataggt ggcagccccc aattgaaaat ctttcatatt tcttgctccc
    13081 ctctacgtca agctttattt taacgatgga ttcttcttct ttcaagcaat aactttgatt
    13141 ttgttaaaaa aaatgtgaga aaattcgcat tttgcttgtt ttttgtaaat taatgagtaa
    13201 aaactcttat aaaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    13261 aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagctt
    13321 ttgaacaccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata
    13381 caaaacggaa aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc
    13441 tgattgaaca ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta
    13501 aaaaaaataa taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat
    13561 aaagatctgc atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga
    13621 tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag
    13681 caacaacgaa acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt
    13741 ctgttcgtgt atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta
    13801 agaatgcccg cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct
    13861 cgtttaatac agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag
    13921 ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt
    13981 catagatact tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca
    14041 attcatcacg tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa
    14101 catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta
    14161 caatagcgac catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat
    14221 ctggtgtgta gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt
    14281 ctggaaggcc agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt
    14341 cggctgaagc aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt
    14401 gagagaactg tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat
    14461 ttgcaacttt tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat
    14521 tcatcaacgg catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat
    14581 tctgattttc tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa
    14641 tggtatgacc accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct
    14701 gatctgcgaa aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag
    14761 ggatcatcaa taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga
    14821 ccccaatcaa tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg
    14881 cgatgttatc catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat
    14941 aataggaaat cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg
    15001 ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca
    15061 aatacatcac gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt
    15121 ttgttacttg ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt
    15181 ctgtggttgc tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg
    15241 ctagtacaaa tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc
    15301 gtaccgttcc ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc
    15361 caccatgtcc tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc
    15421 taagtcctcc tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa
    15481 tagttcttca tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc
    15541 tgcatccatt actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt
    15601 cgttttcaat gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc
    15661 atcaaacaca tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct
    15721 ttgtccacca gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat
    15781 ctctgaaaca aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt
    15841 tgcatccggc ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt
    15901 ttgaggaaca aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt
    15961 tccatttatt tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa
    16021 ggtcgtttta cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg
    16081 gaaatcaata tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt
    16141 gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg
    16201 gataccgatt ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg
    16261 caccatgata aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt
    16321 cataaatgca atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa
    16381 ccaagtgatt gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc
    16441 gacgatcgta ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg
    16501 attttcttca aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc
    16561 tcgaaatacc aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc
    16621 aaagtacatg attccgccga tcaaaataat cattactggg atgacaacta agaagatttt
    16681 tgtcaactga tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc
    16741 attgatcatc atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt
    16801 cgttcttgtg atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc
    16861 tgatttttta tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc
    16921 aaaaaatgta tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa
    16981 acctgtatgc caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga
    17041 ggtaagtgtc ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc
    17101 aattgctgaa gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta
    17161 tttatatact ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc
    17221 tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac
    17281 agtattcatc actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt
    17341 cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc
    17401 gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat
    17461 gttcataaac aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat
    17521 tttagaaaaa taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg
    17581 taacatatct ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc
    17641 tttatcttca aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt
    17701 agcaatcgaa gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag
    17761 tcttttttgt ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggatcctcct
    17821 ttctttcaag atgacacatt gtcatatgac gctgtgtcat tataacacct cctacctgtt
    17881 ttgcaagaca tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga
    17941 ggaatccttc catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa
    18001 ggatgagata aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg
    18061 ctgacagcat cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc
    18121 actcgctttc tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga
    18181 aactttaata tattctaagc tatacaggca ggcgatcaga agcattagcc gatcaactta
    18241 ctgctaatgg ttttcgtgct gtttatatca ttggtgggat ggaagctctt cagtcgagtg
    18301 ttgcttgaat attgcttgtt agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc
    18361 tcttttatac cctgtctttt tttagttatt tattcttcaa ccttatcaat atgacttgat
    18421 agccttttct ttttctcata gttataacaa tcatagcaat aaaaggagaa ggtaagcata
    18481 tggcatctgt tttagtagtc aaaggtcatc ccctaactgc agaagaatcg cgcacagtta
    18541 aggcattgac gtctttttta acaagttata aagaaaatca tccagatgat gaagtgactg
    18601 ttttggaact ttatcgcgat gatattccgg aaatcgatga agaattatta tcaggttggg
    18661 aagcattacg tgctggtgca gaattcactt ccttatcaga aagtcaacaa caaaaaattg
    18721 ctcgtttcaa tgaattgact gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg
    18781 ccttatggaa tctaaatatc ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag
    18841 ctggaaaaac gttccgctat acagaaaacg ggccagaagg attagtgaca ggtaaaaaag
    18901 cactgcacat ccaatctaat ggcggtgtat acaacggaca agactttgcc tctcaatatg
    18961 taaaaggtat tttaaacttt gtaggcatcg atcaagtaga tcagctattc atcgaaggaa
    19021 tcgattatga ccctgaccgt gcagatgagt tgatgcaaaa tgcattagat aaagctgctg
    19081 ctttaggtaa atcattttaa aaattgagat aaatgcatac aaaagagaca gaggaaaaaa
    19141 tcttcaaagc agcatttgta ccttggatat tttcccatct gtctcttttt gttattcttt
    19201 ctgctttttc aacggatttt cttcataaag agaagagatt gttgtgtacc attcaaaaac
    19261 atgagtaatc actactttag ctgctgcata aacaggaatc cccaagatca caccgatgat
    19321 tccgaacaat ttcccagaag tcagcagaac taataaaatc gttactggat ggattgctaa
    19381 ctgattccct agtactaatg gagagatcaa gcgtccttct attgtctgct ctactgcaaa
    19441 aacgatcagt actttgatga tcatgaatgg acctgctaca atggctaaaa agatagccgg
    19501 aatcatcgct aaaaatgatc ctaagtaagg gatcaggttc aaaaaacccg caataatccc
    19561 caacgtgaca gcatactcta aaccaatgac cgcaaaacca atcataaaca tgattgccac
    19621 agcaaaagcc acagttagtt gtcctcttat ataagaagaa acttgactat tcatttcact
    19681 cagcacattc aacgtcggtt tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc
    19741 taattgttta ccgtctttca atagataaaa taagataaac ggcatcgtca gcagagccac
    19801 taaaatagtc gctaccgcgc cgacaaaact tccaagactc tgaactgtac tcttagatat
    19861 atcttggatc atatcgcctg ccgaattcat gaacttatca cctgctgttt ccaactgttc
    19921 gcgaaattgg ttgaacagcg ggtcccgcaa aatctcggtt aatttattat caatcgtatc
    19981 tacatattga gggaagttac tgatgaagct cactgtttgt tcctgaatct tagggatgat
    20041 cactacgctc ccccatgcga ttagtgcaac aaccaaaaca aacagcccaa tgatactgta
    20101 aagccttggt acttttcgct tttcaaagta atcaactacc ggattcatca agtaatacaa
    20161 gattcctgct aaaatgatag gcagacctac gattgctaaa aattgccaga ttggtgcaaa
    20221 caaatatgaa acttttgtaa ataaaaagac gatcagcaat attaacaata caacaagtaa
    20281 ggctgtgact acttgattat ttaaaaacca gcgccaaaac cacgataccg gtctgttctt
    20341 cttatttttt ccttccataa ttcctcctgt tagttataaa aaatttggct tcccaaacca
    20401 atagcaggca gtctatgcgg atgaagatag attccatttg caatttgtcc ttcttctgga
    20461 caagcatcaa aaatcaatga tacgtgagct actgtctcta aattcccatt ggccatcgga
    20521 ccgacataat cgatcgtata tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa
    20581 gaaaaaggtt cgtcttttga tacttcttga atcaccgaat attttcgtaa ggcagctgtt
    20641 gctgttttat cgaacaaaat cagcatgggt tcttccttgt ttattgcttc tgtcccaatt
    20701 tctgtaatct ttgcctgaat cattgcactc tctcctttaa ttgctttcaa aaacagtata
    20761 acacattctc attctttagc cgtgtggaat aattatgtga tagaataaaa acgaaaacta
    20821 tttcattaca attaggagga atcagctcat atgatcgaaa agataatttt aggaacctat
    20881 acgcgtcgcg aaagtaaagg aatctatacg atcgaccttg atacagaaaa agaagaatta
    20941 gccaaccttt cattagctgt tgaagaaaat agccctacct atgtagcaaa aagtaaagct
    21001 ggcaatcttt acacagttac tagtgtggat ggattaggtg gtgccggtgc gtacgataag
    21061 gattttcatt ttttgaatgc ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt
    21121 gatgaagcac gtcaattact atacggggcg aactatcata aaggtgaagt aaatgtctac
    21181 catatattag gagatggcga actgaaagca gcagattcaa tattccatca agaagcgaca
    21241 ggtcctcata aaaaccaaga tcatgcgcat gtccactata cggatctgac tcctgaccaa
    21301 cgattagtcg tatgtgatct tggtacagat cgtgtgtata cttatgatgt gtccgaaaac
    21361 ggcaaattga atcttgtaac taccttcact gcagagccag gaacaggccc tcgccatctt
    21421 gtctttcatc caaatggaca attcgcttat ttatttggtg aattagacag cacagtccgc
    21481 gtgctttctt ataataaaga aacaggtgct tttgaagaat tacagaaaat atcgaccttg
    21541 ccagaagaat tcgacggtga aaatggaggg gcagctatcc gcatttcaaa tgatggcaag
    21601 ttcctttatg cttctaaccg aggacataac tcgattgctg tattcgcagt ttttgaagat
    21661 ggttcccaca ttcaaaacaa gcaaatcatc tcaacagaag gggattttcc ccgcgatttc
    21721 gcactagatc caagcaataa ttttgtcgtg tgtgccaatc agaacaccga taatctgaca
    21781 ttatatcgcc gtcatcctga aacaggatta ctggaattac tgaaaaaaga tgtctatgta
    21841 ccagaatgtg tctgtgttta ttttgatcgt tagtaagaaa aggagaatga aatgaatcag
    21901 caagacgtat tagacagact acgggaagaa ctaaatattc ctttctttga tgggaaaatc
    21961 gaagataaag agtatagcga agaggaatat cagaaactca aagccgattt acagaactac
    22021 tttgaacaat atgtacagaa tgtagaaaac taatcagctt tccatactat ctgaaaagaa
    22081 catcaggaca agacttttcc gtctatcctg atgttctttt ctacaaataa aaaaccgccg
    22141 agatggttag actcgacgga aaaggagtat ttactcgatt ttgagtaagt acaaggttgt
    22201 tgttggttat aggtatgtca ttatcataaa ggattttgat cactctatca taaaggtaat
    22261 attacattta tattagataa ctctagtttt tattgttatc aaagatttct tttatttttt
    22321 caacaacaaa ttagtcattt tttggatggc agctgtcaat tggtctgtcg cttggtcttg
    22381 ttttgcttca gatgtattga atagatcttc atctacccct gattcaagat cgatactttg
    22441 gatcatttct tcacagcctg ctacatatga aagataagct ttttcaaaat ttttatgaat
    22501 gcccattgct tttgctggtg gacgcaaagt actgactttc ttaagcattg cccgatattt
    22561 ttttgttcct tcttcaaata acaggatgat ctcttcaaga gtcgatttgt ccagttcact
    22621 tgtttttcct tcatcaattg cgtttctgat cacttcatat ttcggatgca ttccttctcc
    22681 gattgcttct gtttccttga cgatttgatc gatggactgc acataatagc caatattcgg
    22741 taacatgttc gttttcctac tttctccatt gttgttatta tttattctac ttgtttttat
    22801 cctcaaatga caagtacttg aaaaaattta atggtttcta tacagctcgc cattcttatt
    22861 cgtgctcaaa gaaaccatct gctaaactat attgtttccc tgcgacaatg tcatattgga
    22921 tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct tctttatacc
    22981 actgtccgtt ttgatagtac agttctcgtt caaacgccgg aagttcctgt tctaatgata
    23041 aaagcagctg ataaaagcca tttgttgtct gattcgtctg ttccattaga ttcgctgcaa
    23101 aataaaacgg actggtgatt gcatcgtgag tcgtttgctt ttctaattct cctttactgt
    23161 cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc attttttgct tggatcgtat
    23221 ctgaatagat ccctggaaga tgatcccccc aaaaaacaac taaagtcctt ctagaaagat
    23281 tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata tcttgcagat
    23341 aattctctaa gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag tctagattat
    23401 catactttcc atcgtagggc atatgtgtct gcatcgtgac taaatgaatg aactgaggtg
    23461 ttttttcttc cttgagaagt gtcattactt ctttgtaagc tgactcatca gaaatatatg
    23521 gattattctc tatcgtatcc gtataggtca tcgtacgctc gctgatgaac tggtcaaatc
    23581 ctaatgtctg ataaacatcc tctcttttat acatcgaagt attgtatgga tggatcgctg
    23641 tcgtctggta tgattgtgcg tttaacgctg aaacgataga aggaagctga tccatttttg
    23701 gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata gaaaaaccag
    23761 tcaatgcttc aaattcgata tttgctgttc caccgccata gttttgagaa agcatattgc
    23821 cactatatgt ttgatcagct acttcataat aatctgccaa tggctcacca gatacttcta
    23881 cgccgtttaa tcgactagga tctgagaaac tttcactcat cacgaacacg atgttcggcg
    23941 actcttcctc tacagtttta tttttttcat cggctagctt ctggtatttt tcagtgattt
    24001 ctttgatctt ttctttcgaa tatccttcag gttcatccat tggttctacc ttcagattgt
    24061 acaaaaaccc accgataaaa ccagtgttat agtagttcat tttctggctg taaggtatcc
    24121 atagtgccgt tttattatat gcttttcgca acaaattatc gggattgttg aaattactga
    24181 tataccctag cagtccaatt gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc
    24241 ttcctttttt caggcttcga aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg
    24301 cagctaagat cactgtaaac agcatagtgc cggtcatatc tttcagcaat ccgatttcag
    24361 tgatcatctt caaatcgtct ggataaatcg gttcttgccg ataactcatt ttcatataat
    24421 ttgcaaaacc taatacaccg atggctacga catacgtaag ggaacctaaa gggacagatc
    24481 ctgctagtgc aatcagaaaa ataagaataa tcagcaagac gagacaggct aagaagaatt
    24541 tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc tacggataga tcattttgac
    24601 accactgcaa aaataaatta ctaaaaacaa caagaaaaaa gatactgatg attgctgctg
    24661 ttccttttaa aatctttttc tgtttggtcg aaagtctgct tatatatctt ttcaaatgct
    24721 tgctccttca tttctatcaa taaaagaatg ttgcttctct tattttagtt gtacgaaatt
    24781 cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa tattctcttt ctttatggcc
    24841 tttctggttt ctttaataag cggacctttc gtactccaga aattttttct agctgttgtt
    24901 tcacttgatt gagctgttca aaatcagtac tgtctatgtc aatcaatatg tagacatgct
    24961 cattcttatg gttacgcgat atatctgcga tcgctagctc gttctcattc agcagagaaa
    25021 atatctctgc taatatattg gttttctttt gataaaatat agtgaatcga aacggcgctt
    25081 ggaaaaacat tcgtgcggat ggataattga ccgcttctct aaccgtaccg aacaaaagat
    25141 aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc
    25201 cccctagatg aggcaacata agtatacggt cattttctaa gaactcttct tctggaaaat
    25261 cagtcaagta tttagccaga tgttgttttt ccaatgcttg cataacagca tatttatcaa
    25321 caatttcact tcttcctaca ttgataagta cagcatcttt tttcattttt tctatattcg
    25381 cctgatcgat caatcctttt gtatcttctg tcaacggcag catgactacg atgtaatccg
    25441 aggtagaaag aactgtttct aaatctgctt gccgaacata atccaactgg gcatcgcgga
    25501 tagaataacc caagacatcc atccctaagc tatagcaact tagcgctact tttgtgccta
    25561 tcgctccaag accaagaaga ccgatggttt tgccttgcaa ttctcgtccc acataagcac
    25621 ttcttttgtt ttctgcctgt tctaaaatat taggacctgt tagtgtttgt accatacgac
    25681 ttgcttcgat tatcggacgg gaagataaga gcaaacagca caaaacaagc tcttttactg
    25741 cattagcatt tacacctggt gtattcatga cgatcgtccc gttttcagaa gctttttcaa
    25801 cgttgatcgt gttcacccca acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc
    25861 attcatcttt cactttcgta ctacgaataa ctattccttc ggctggcaaa tcatctatta
    25921 ttttgaaata ttccccttgt aacttttcta tctcttgttc tggaatagca tcaatcaaat
    25981 gaacgtgata cataaaaccc cctccttact aacaagtatc tcgaaatttc tgcaattaga
    26041 caactgttta ccatttgact tcctagaagc aaaaagagca gttagagcag agtaggctga
    26101 gtgataaaaa taaggctatg acaagttact gccataagcc ttattttcga gagcaaaata
    26161 ttgagggtga gatatcttga taaatggtgt tccgttagct gacattgata ataagactaa
    26221 atttcaaaaa agttgaaaag caaatttcgt caatctcttc ttatttctta aaactaagtg
    26281 gctttttcac aacctctttt tatctaataa ccactgtttc caactcaact aattttgccc
    26341 acgtttcaat ttgatctgtt gttagattta gcgagacaac ggtatgatga ccgccacctg
    26401 cctgaatcca tgaatggaca ccttcatgga aattcggttt agttttccat aacacacgtg
    26461 caacaggtaa attaggtgct tcagtcgttg gttcaaaggc ttctacttca ttgatcagca
    26521 gtttataatg cgtaccgaaa tctgccatcg atacgactac cccttctcct gctttcccat
    26581 caaatacaag acgcgccgga tcttctctat ctcccattga taaaggagaa acaacgattc
    26641 ttggtttcgt cgcagcaagc gtcggatcta cttccatcat atgggattcc aagattgctt
    26701 cttggccact agctagctcg taagtataat cttccatgaa tcctgtatct ttccctcgag
    26761 ccatgatttt cagcaaccga tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac
    26821 cgtatccttc tgccatcaaa cgttgtacag ctagtccagg tagttgtttc attccataca
    26881 ggtcttcaaa attagtcgta aaagcgttat agcctccttc ttctaagaaa cggcgaatac
    26941 cgatttcctg ctgagcttgt acttttacat gttcctccca tgtcttttct tcataatcac
    27001 catagtcgaa ggtatacaat tccttgtact cttcaaataa ttgatcgact tcttcgtctg
    27061 atacgcgatc gattacttgt acaagatctc cgataccaaa ataatcaaca gtccaaccaa
    27121 attgaatctg tgcttccact ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac
    27181 caaaacgagc cacttttatt ccaaaacttt cattataggc tactgcaaca tccatccaat
    27241 cagcaatctc tttttggatc tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt
    27301 tatttaatcg agcattgatg aacccatatt cgcggtcacc atgagcggat tgatttaaat
    27361 tcatgaaatc catatcgatc gttttccaag gaatgctttc gttatattgc gtagctaaat
    27421 gaagcaatgg tttttgtaga agctttgtcc cgcggatcca catttttgct ggtgagaaag
    27481 tatgcatcca agtgatcaca cctgctactt cttctcgata attgacctct ttcatgattt
    27541 ttgtgatcgt atcagcagta actgctaatt cttgaaggcg gattggataa ggcaattggc
    27601 cattttcatt cagttcatcg accatttctt gtgcatgttt ctttacttct cgcagtgctt
    27661 cttccccata taaatgctgt gatcctacaa caaaccaaaa ttctttttct ccaatattca
    27721 acataattag ccactccttt tatttgtttt gtccgtaata cgcatttttt ccgtgttttc
    27781 gtaaataatg tttatctaaa atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc
    27841 tggtaaataa attcatcttg catacttcat ctagtacgac tgcgttcatt accgcacttt
    27901 gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa aagtacccct ggaattgcta
    27961 agggatcaat tttgcgttct ttgaatgttt ctatgataac ttttcctgtt tcatattcgt
    28021 acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag
    28081 tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg
    28141 cccatgtcga gtgagtatgg acgattcctc cgatttgagg aaatgaacga tataaaacag
    28201 catgagtagg cgtatccgaa gagggattga gtttaccttc aatgatatga ttgtccaagt
    28261 ccacaacgac catgtcgtct gctgtcatct cttcataact gactccgcta ggtttgataa
    28321 cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc
    28381 cgtgtttagg caagtcaaga tttgcttggt atacttcttc tttcagttgt tccagcattt
    28441 tctatcactc ctttttcttg caattgatga atcgctgttt tttcaatgtc gatcccctca
    28501 cggtaacgtc tcataaatat ctgatagcct gctatttctt ctgctgaagg tgtgatggtg
    28561 attccttggt cttgtccaaa cacgtcttct gccaagaagt tttctaaaga cttcccatgt
    28621 cgatccatca tataggctgc taataacgcg attccccacg ctccaccttc tccggctgtg
    28681 tccatcactg taactggtgc ctccatagca gatgctagaa tccgctgtcc aacttctggt
    28741 gttttaaaaa tccccccatg ccccactaaa cgatcaattt ggacattctc agatttcaat
    28801 atatccattc cgattctcat tgcaccaaaa gcactggcta aatggatccg catgaaattc
    28861 gctaaatcaa aacggctacc tggtgtacgt acaaataatg gccttccttc atttactcct
    28921 gtaatgtttt caccggaaaa ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct
    28981 tctagcgctt tgttgaataa taccgtgaat aatttatctc tgcttatctc catacccaaa
    29041 gaattggtaa attcttcaaa aatactgacc caagcattga tatccgaaga gcagttattc
    29101 gtatgaacca tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg
    29161 acattttcta gttctttttc taatacgatc atagcgaagg cggaagtacc agccgaaatg
    29221 ttccctgtcc gtttggctac actgttcgta gcgaccatac cggttcctgc atccccttct
    29281 ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag ataagcccct
    29341 gattcgctta acttgccagc ttcttctcct gctactagaa ctttaggcaa aatgtgatcc
    29401 aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa aaatattttt catttcttga
    29461 tggtaggttt tcgttgaagg atcgatagga aacatgcctg atgcatctcc tacgcctagt
    29521 actttctcac cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt
    29581 tctttcacat gttcttcttt attcaaaata gattgataca aatgagcgat actccatctc
    29641 tgcggaatat tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc agtgattgta
    29701 tttcgccaag tacgaaatgg tactagtaac tgttccttgc tatcaaatgc tagataaccg
    29761 tgcatcattg cactgaaccc aatcgacccg attgtcgtaa gattctcgcc atagatttcg
    29821 aatacttctg ctgccatttt tcggtaactg acgcgcagtc ctttccaaat atcttctaat
    29881 gaataggtcc agataccatt ttctagtttg ttttcccact cataactacc agcggcaata
    29941 ggagtaaaag atgaatcaat caatacagct ttgatcctag ttgatccaaa ctcaatccct
    30001 aaagatgtac gaccatctat gacatctaac atactttgcg catttacttc cttcataatg
    30061 atttaactcc ctccttctcg tcaaacctga tcaaaccgtc tttttagtag cgcttacaat
    30121 gattattgta tacttttgta cgtacatgtc aagcggttat ttacaagaat acaataaaaa
    30181 acacttgttt tgtacgtaca taagttataa aatgaaattg cttacaaaat ctgatcaacc
    30241 gccttctcga aattctagaa ggagagaata cttatgaaaa aagaaaaaaa agtttcctca
    30301 tcgtttattt atttttttgg cgcatttggc ggcattctat ttggatatga tattggtgtt
    30361 atgaccggcg ccttaccttt tttacagcat gattggggtt tggcaggtaa agcttctttg
    30421 ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt
    30481 cttctttctg ataagcttgg acgacgaaaa atgattcttc tttctgcact tatttttatg
    30541 gctggttctg tcctttctgc atctgcccct cacaatggtt cttattttct gattgcagca
    30601 aggattcttc taggtttagc tgttggcgct gcatctgccc tagttcctgc ttatatgtct
    30661 gaaatggctc ctgctagact aaggggaaga ttgtcaggga tcaatcaagt gatgattgct
    30721 tctggcatgc tcctttccta tgttgcagat tatttgctaa aagtgttgcc tgaaacgatg
    30781 gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc tgattttatt tttcggtgtt
    30841 ttagctctac cagagtcacc gcgttttctt atgcaatctg gccgcttgga agaggcaaaa
    30901 agagtattga actatattcg gacaccaaaa gaagctgaac aggaattcga acaaatccag
    30961 ctgaatgtca aacaagaaaa gacaaccgga acttcttggc atactttgtt tttagaaaaa
    31021 tatcgttcct tagtcattgc cggtattggt gtagcagtat tccagcaatt tcaaggagct
    31081 aatgctattt tttactatat tccgctgatc gtagaaaaag cgactgggca tgcagcgagc
    31141 gatgcattga tgtggccgat catccaagga atcattttag ttgctggttc tttgctcttt
    31201 ttggttattg cagacaagtt caatcggcga actttattga aaataggcgg tagcgtgatg
    31261 ggactttctt tcatcctacc agctgtttta ggaactgtac tggatgctca tacaaattcg
    31321 ttgttgattc tcttgttttt atgtatttat gtcgcatttt attcttgcac atgggctcca
    31381 ctgacatggg taatcgtagg cgaaatattc ccactagctg tccgcgggcg agcatctggc
    31441 ctagcctctt cttttaattg gattggttct ttcctagttg gcttgctttt tcctgtgatg
    31501 actgcttcta tgtctcaaga aatcgttttc ggcatttttg ggatcatctg ctttttaggg
    31561 gttttattta ttcaagagat cgtaccagaa actcgtggga aatctttaga ggaaatcgaa
    31621 caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc ttaaagaggt
    31681 tgtgacagaa gtggtcagct tcaagaaata agaaggaacc gcttagtttc cagtcacaag
    31741 gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg
    31801 taggagaaca ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg agcgatgaca
    31861 aaagctctca caaacattgc gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc
    31921 gtctcttatt tttcgttttc tttcactgtt tcccgttcaa ctagtacagg ttcatagaga
    31981 atatttggct gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt
    32041 cccaactttt cttttggatg cgtcaatgtt gtcaattttt tattccctaa atggcttaaa
    32101 gaagaatcgt ctgttcctac gatcgatagg tcatctggaa tatgatagcc attttctgtc
    32161 aacttatcta gcattaaact agctatttga tcgttatagc aaatgatgcc atctaaagct
    32221 tgttcttctt tcaaccgttg tactgttttt tcagcgattg tttctcgtgt ttccgtggtg
    32281 aaagtaatga tatcttccgt caaaaaacgc acattatact gttcacacgc gctgataaaa
    32341 cctttcatcc tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa cagacgctga
    32401 tgtccttttt ttatcaaata ttctgctgcc ataaaaccga ctttcacatc atctaaacaa
    32461 atataagagg catttaactc ttcataagca gcattgatca tcaccatcgg aatgccttgt
    32521 tctctcaaag caacataaag tgctaagttt gggttatact gattactttt cgtaggctca
    32581 acgatcaaac catctacccc ataagcaatc attttcttca aacactcttt ctccaattcg
    32641 tgatcgttat tcgtactggc taaaagcaat gaatacccag attctcgtaa ttcattttcg
    32701 attcctcgga taatcgacgg aaaaatataa tcagagagat atgtggtgat gacaccgatc
    32761 gtttttgttc tgttcaatgg tgttttttct ttcttatagc tgtcatctac atatgtgccc
    32821 gacccttttt ctttacgcag atagccctca tttaccaaca cagcgattgc ttgccgaatc
    32881 gtatgtctgc tgacgtcata tatttcttgc agcttgaatt ctgagggaat ggttgtattt
    32941 acttcgtatt cctcacgaag gatcttttgt tttatctctt ctgcaatatc ttgatatttt
    33001 gccatttgcc ttgccccttt ttatttgtac gtacatatat cataacgagt ctgcaaggtt
    33061 tattcaagca aaaatcaagg tttcttgtgc tcataaggca tacggaacag gttcaccaaa
    33121 ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt
    33181 gtctaacgga tccccttttt ggttcttctc tggtcgagca tgatagatca aaatatcaaa
    33241 gcacccgtct tctgaaaccg taaacgagtt gtgaccagga ccaaattgct tatttttttc
    33301 agaagaaacg aatactggct ccgataattt atgccatgat tcttttaata aaagattctt
    33361 gtgctcatca gcccacagca ggcccatggc atagttttca tctgttgcac taccagaata
    33421 cgtaatgaat attttcccat tgcgagtaag gactgctgga ccttcattta ctgaaaaacc
    33481 ccgtttttcc cattcatact caggtataga aaggaggatc tgcttcccct ttaacgtcca
    33541 aggattatcc atttcggata gataaagatt agaattccca gtaatttcag gatcttgttg
    33601 ggcccacaca taatatagct ttccttgaga agagaaagtt gttccgtcta aactgaaagt
    33661 ttcccttttc gtttttattt gtcccttttc tatccaatct gtattcattg gatctatatg
    33721 ctgattttca agtacgaaca tgcggtgatg atgagattca tcacgtatag ttttatgatt
    33781 gcttgcagca aagtaaatat accattttcc attgatataa tggacttcag gtgcccagat
    33841 caattcactc atcggtcctt cttcatgagc ctgccataca atcaggcttg cagcatgttc
    33901 taattcattc agtgactttg cccttcttaa ttcaatcact tggtagcctg gaaccgatcc
    33961 tgtaaaataa taataaccat ctgtatgttt atatacccaa ggatctgccc gttgccgaac
    34021 gataggatta ttataaagtg tcttcatcat aacaaatttc ctcacatttt ctttattcta
    34081 attacattcc aagagtattt ttctatccct acatttaatt cgtcatgttt ttctattgaa
    34141 aaatagttta tttcaggttt tattttttct ggttcttcac gtgtattttt gtccaacaga
    34201 tcgctactgg ttaatgtgta gtgctctcct tgatctagca aattgaaatc atgttcaatc
    34261 gttatctttt gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg attgccgtta
    34321 tctacgatca cactgtccac ataaggaaca ttttttatct tttcacaatc ataaacaggg
    34381 caaatgattt gttgtttcaa tacttgacct ttagcatatt tcgataagtg ttgcatcaca
    34441 taataagttg gattcctcca tgcttgtcct ttagcatctg tcagtattaa aggtattgtg
    34501 tttactaaca atgattgaca actaatcttt acgcgatctg cgtgtcgtaa aatagccagc
    34561 cctaatgaac caaataataa tgtatcttcc atagtaaagt aacaccaatc gatgggacta
    34621 ccgacttcaa aatccctatg tcttttttcc ggttgggcat gtacattcca ttcatcaaac
    34681 gctagataca ttgttttctc acttctcaaa tatcctttta cataatcaca agtagcaacg
    34741 atttcttcaa tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca
    34801 cgctcatttt taggaggttt agtcaagtct tcttcatcat acttatcaat atagttatgc
    34861 aaagctaaat aatctacatt ctcataagct tcttctaatg cgatacgatc ccattcaggg
    34921 tacgatggta aacgtggcgt agagcttcca actaagattg tttcaattga atcatccact
    34981 aacttcatga cttttgaggc ttcatttgct aacctaccat attcataagc actcttagcc
    35041 cccacttgcc atggaccgtc tagctcattc cctaaacacc ataatttaac gccaaaaggc
    35101 tttttttgcc cattttttat tcttaaatca ctccagaagg ttccttttgg aaaattacaa
    35161 tattctacta aattttttgc ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc
    35221 ggtttcgctt gtacatgact gatccattta aaaaattcgt ggagtccaaa agaatttggt
    35281 tctatacctc gccatgctaa atcaattttt tttggtcgtt gttctttcct acctacagta
    35341 tccacccaat cataaccaga tgtaaagttt cctccaggat agcgaatgac acctaattct
    35401 aattccttta cataagagag gacatcttgt ctaaacccgt caccatcagc tgtttcatgg
    35461 cccggttcat aaatccctgt atagacgaca cttcccatat gctcaacaaa tgagccaaac
    35521 agccgagaac tgatctctcc taccgtaaaa tcctctctga tttttacttt cacttcttcg
    35581 tccaacagct ttccctacct ttcatttcct atagtcaaga taatagatac aagtttccat
    35641 acgccaccag caaagataaa aataaaagga gggaaaagaa atatgagcag tacaaatcct
    35701 aataacaata cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt ccatgcactt
    35761 tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag aaatcacata tatccaggat
    35821 aaaagaaaca tggaataaat aagtaaaact acatatccac taattgaaag tacgattgtc
    35881 tgttcttcta ataattgatt aacaagaaaa taaataatga tcgttaacag actaaggaac
    35941 aagttcttac caaaatgttg ctttattcct tgaaagaaaa aataaatgat atttcctgat
    36001 ccctcacctt tccattgcgc cattacataa aaaacagcac atgtagcagg cacgatagta
    36061 ataacaggca aacacacgat tacccagaga atacctaata agatgtaatc agaaaccaca
    36121 atcaactgtc cataaagttt agtctcaact attttcatct tcctcactct ttcattccag
    36181 acatagctgt tccttgaaca aaatatttct gtgcaaaaat ataaaccact agtaatggca
    36241 gcattgaaat aaccgttcca gccattacag gtccataaaa agaaatgttc tgccctgtaa
    36301 ataaagctaa ccctgctggt aaagttctca tttttgaact acttgtcaat atcaatggat
    36361 aaagcaagtc attccaacta ttcatcagcg tgaaaatccc taatgataaa agagtaggtt
    36421 tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg
    36481 ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga acgcatcatg aagataccaa
    36541 atgcagttgt catacgagga atgatcaaac ctgcataagt atcaagcatg ccaagcaaat
    36601 tgacttcaat aaatagagga atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa
    36661 cgaaatagaa caaaactgat tttcctttga aacgcatacg agcaaatgca taccccgcaa
    36721 gagaatcaaa gaatacagaa gtgatcactg tccctccagc aaaaatcact gtatttttga
    36781 tgtaatctaa taaagggatc gttgtccaaa ccttaatata gttatctagg gtatagctat
    36841 ctgcaaatat tctaggcgga aatgagataa tgtctttttc gtgtttaaaa gaagaaataa
    36901 tcagccacaa aaaaggaaac gcaataacca acaatataac tagcatacta atcgttcgaa
    36961 tgattttttt accactaact gcttttcgac tattcacagt aactgtaatc gagctttctg
    37021 gtgccataca ggtttccctc ctatttcatt tgtttttctt tccgtgacat gtacacatta
    37081 gtggagatag ctatagttgc aacaatgata aacaaaaccg aagacagtgc tgatgcatag
    37141 cccaattcat aaggtgctgt aaatcctcga ctgtaaatat attgaacagc tgtttctgtc
    37201 ttaaattgtg ggccaccagc ggttgcaaca tatacttggt caaacacttg catcgaacca
    37261 atcagattcg ttaaaataca aaaacctaag gagggaacga tctgaggtag cgtaatgtta
    37321 aaaaactgct gtcgatttgt tgcaccatcc atttgagcag attcatacaa agagggagag
    37381 attccctgaa tagcagtcaa gagaatcgtc atagtaacac caaaattctt ccaaacagtc
    37441 atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag
    37501 ccgacttgtg aaaacaagta aggaatcgcc cctacattcg gatcaagcaa catagaaaaa
    37561 ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga acgacaaaaa
    37621 cgattgaatc tggtattttt agataatgct actgctgcta ataaaccaac gataatttgt
    37681 gttggcgttt cgagtacggt gaaatataaa gaatgaatca acgaattaac tgctcgttca
    37741 tcactaaata tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt aaagaaaata
    37801 ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg gtatgacagt aaataataat
    37861 aaaactaaaa aagatggagt taaaaacaaa tacgcagcac gatcaggacg attccaatat
    37921 ttggtgaatc cgttcatata gtccacctca tctttcattt tgataaaata tacaatcttg
    37981 gtttaaacga atctgaataa gatatccgaa caatcgctgt gatttttgca ataacaccta
    38041 tctgcaaata ctaggtcgat cattcagata tctgtatctt gccaacagtc tatcctgtat
    38101 aaagttctgt actatttttg cagtaattga ttgatggcat catctgcatc gttcatgagt
    38161 gtctcaggat cttctcctgc taataatttt tcaatcagtg gattgatgat atcgttattg
    38221 atcgcagaga tttgggtaaa acctggtaaa tatggctctg catagtccat ttgtttcgat
    38281 aattcagata caataggatc attttttact tcttcatctt ttgctactga atccaagtag
    38341 gcaggaaaac cattttcgat actccatttt ttaccaattt cagttgtatt ccaatatttc
    38401 aaaaactcat agacagcttc ttttttatta ttatctgtac ttgccggaat ggcatatcct
    38461 acgccatcta aaatagctgt ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca
    38521 ttataatcaa tctcgtttgc tttcaatccg ttatttaacc atggcccatt gatttcaatg
    38581 gctagttgac cagcattcat taaattgtcc atttcggcgc cagaaatcga ttctggacca
    38641 gtcttgtcta catggatcat tttttggatc gcttttaacg ttttaaggtt ctcaggagac
    38701 gcaaattcag acttagtata atcatcgttc actaatttac cgccatttgc taacatccag
    38761 ttatataacg gtgcagttcc atccttattg aagacaaaac ctgatacatt tttactacta
    38821 tcagttaatt ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta
    38881 tctaatccag cttgtttaaa caaatcatta ttccaataca tatacatgcc ttgtacctgc
    38941 atggggacaa aatactgttt accttctact tcacctaatt caatagccgt ttctttaaaa
    39001 tcagatttat ctactccatc atattcccag aaatcgtcta atggttgtac cgctccgttt
    39061 tctacatact gtgcaaaatc tggataattt aatgcaataa agtcaggtgc ttttttagaa
    39121 gaaatcgctg taggaagttt ttcattcaaa ttggcccaag tcatgatatc catttcaatt
    39181 ttgatgtttt tatcatttgt tttattaaat tcattaacga tttcttgcaa tatttcacca
    39241 tcagatgcag taaacccatt ccaaaaagtc aaagtcactt ctttatcaga atcagattct
    39301 gctgaacttc cgccaccgca agctcctaaa atacctgttg cagctaccaa caaagagcca
    39361 attaaccatt ttttaccttt cattttttgt tcctccttca tctgataaac gacatctgac
    39421 cacattccaa gatagcggtt tcaataaaca attgattttc ccatcttcag cttttacatt
    39481 tgttgaatga cttggtttta cttgttcact tttaggaaga ttagttgctt ttgtatcaaa
    39541 tccactcatt tcagaaaaat caatcactcc ctctaatgat aattcagaca agtgaacagt
    39601 tagattcatt tcttcttctc cacggttaac tgcaaatatg acaagttctt tgtcatttat
    39661 tacggctata ctttctaaat acggcacttc ttcaaaatct tccgtcgcat agtttgctga
    39721 acgaatttgt gggcttaaca cctctccttg tccatacaca gcagcttgca taaatggata
    39781 atagatcgtc tgtctccaag cttctccgtt tgtttctgtc ctgattggtg caataacatt
    39841 taccaattga gcaagacaag caatctttac tctatctgca tgtttcaata aagtgattaa
    39901 tagacagcct aatagtaatg catcttcaaa attatagtta tcttctagta aaggaggagc
    39961 aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa tattccattc
    40021 atcaaaggac aggttgatct gcttcttact atgtttcttc gccttgactg tatcgcacat
    40081 cgctgtcact tcagaaatga accgatccat gtctaaggaa cgtgcaagat aatgagaaag
    40141 atcatttttt tggttgccat aatattgatg cagagacaaa taatctattt gatcgtacgc
    40201 ttgatcaaga actgttaact cccaatcacc aaatgtaggc atttgccgat tcgaacttcc
    40261 gcataaaacc acctcaattg aatcatctac gagtttcatc gccttagccg tttcctctgc
    40321 taaccgtcca tattcttccg ctgttttatg tccaatctgc caaggaccgt ccatttcatt
    40381 ccctaaacac catgtcttaa ttccaaaagg gctctcttga ccattcgaac gccttaaatc
    40441 actccaataa gttcctttag gaaaattaca atattcgact aaattacgag ctgcatctac
    40501 tccacgagta cccagattga cagccatatt gatttcggcc cccacatgct gacaccagtt
    40561 ataaaattca tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta aatccaacct
    40621 tctaggtcta tttgctatgg gcccaatact gtcttcccaa cgaaatcctg acacaaaatt
    40681 tccaccagga tatctaatca tgggaatgtt caattctttt accaagtgaa tgacgtcttg
    40741 gcgaaatcca cttttattag ctgatggatg atctggctga taaatccctt catatactgc
    40801 tcgtcccata tgttcaataa atgaactgta caaccgcgga tcaactttgc ctacacgctt
    40861 gacactacta atcaacattg tggcttccat aactgacctc ctttaattta tacatgaata
    40921 atacacctcg ttgaaa
//
LOCUS       DACEJR010000015        40608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEJR010000015 DACEJR010000000
VERSION     DACEJR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.015"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..1889)
                     /locus_tag="IW651_000851"
     CDS             complement(6..1889)
                     /locus_tag="IW651_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0813599.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2155..3030)
                     /locus_tag="IW651_000852"
     CDS             complement(2155..3030)
                     /locus_tag="IW651_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0813600.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(3139..4908)
                     /gene="aspS"
                     /locus_tag="IW651_000853"
     CDS             complement(3139..4908)
                     /gene="aspS"
                     /locus_tag="IW651_000853"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0813601.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4923..6215)
                     /locus_tag="IW651_000854"
     CDS             complement(4923..6215)
                     /locus_tag="IW651_000854"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0813602.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6609..7556
                     /locus_tag="IW651_000855"
     CDS             6609..7556
                     /locus_tag="IW651_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0813603.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7645..8904)
                     /locus_tag="IW651_000856"
     CDS             complement(7645..8904)
                     /locus_tag="IW651_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0813604.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9075..9521)
                     /locus_tag="IW651_000857"
     CDS             complement(9075..9521)
                     /locus_tag="IW651_000857"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0813605.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(9532..11745)
                     /locus_tag="IW651_000858"
     CDS             complement(9532..11745)
                     /locus_tag="IW651_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0813606.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(11978..12742)
                     /locus_tag="IW651_000859"
     CDS             complement(11978..12742)
                     /locus_tag="IW651_000859"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0813607.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(12743..13690)
                     /gene="prmA"
                     /locus_tag="IW651_000860"
     CDS             complement(12743..13690)
                     /gene="prmA"
                     /locus_tag="IW651_000860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0813608.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(13703..14191)
                     /locus_tag="IW651_000861"
     CDS             complement(13703..14191)
                     /locus_tag="IW651_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0813609.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(14157..14828)
                     /locus_tag="IW651_000862"
     CDS             complement(14157..14828)
                     /locus_tag="IW651_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0813610.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            14966..16252
                     /locus_tag="IW651_000863"
     CDS             14966..16252
                     /locus_tag="IW651_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0813611.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            16324..16518
                     /gene="ssrS"
                     /locus_tag="IW651_000864"
     ncRNA           16324..16518
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW651_000864"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16596..16880
                     /locus_tag="IW651_000865"
     CDS             16596..16880
                     /locus_tag="IW651_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813612.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            16953..17432
                     /locus_tag="IW651_000866"
     CDS             16953..17432
                     /locus_tag="IW651_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0813613.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            17655..17798
                     /locus_tag="IW651_000867"
     CDS             17655..17798
                     /locus_tag="IW651_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0813614.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            18376..19959
                     /locus_tag="IW651_000868"
     CDS             18376..19959
                     /locus_tag="IW651_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0813615.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20396..21580)
                     /locus_tag="IW651_000869"
     CDS             complement(20396..21580)
                     /locus_tag="IW651_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0813616.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21605..22612)
                     /locus_tag="IW651_000870"
     CDS             complement(21605..22612)
                     /locus_tag="IW651_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0813617.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(22784..23143)
                     /locus_tag="IW651_000871"
     CDS             complement(22784..23143)
                     /locus_tag="IW651_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813618.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(23115..23486)
                     /locus_tag="IW651_000872"
     CDS             complement(23115..23486)
                     /locus_tag="IW651_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0813619.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(23503..23805)
                     /locus_tag="IW651_000873"
     CDS             complement(23503..23805)
                     /locus_tag="IW651_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813620.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(23822..24292)
                     /locus_tag="IW651_000874"
     CDS             complement(23822..24292)
                     /locus_tag="IW651_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0813621.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(24267..24545)
                     /locus_tag="IW651_000875"
     CDS             complement(24267..24545)
                     /locus_tag="IW651_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0813622.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(24542..25591)
                     /locus_tag="IW651_000876"
     CDS             complement(24542..25591)
                     /locus_tag="IW651_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0813623.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(25548..26477)
                     /gene="cpaF"
                     /locus_tag="IW651_000877"
     CDS             complement(25548..26477)
                     /gene="cpaF"
                     /locus_tag="IW651_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0813624.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            26609..28363
                     /gene="ade"
                     /locus_tag="IW651_000878"
     CDS             26609..28363
                     /gene="ade"
                     /locus_tag="IW651_000878"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0813625.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(28461..29123)
                     /gene="trmB"
                     /locus_tag="IW651_000879"
     CDS             complement(28461..29123)
                     /gene="trmB"
                     /locus_tag="IW651_000879"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0813626.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29193..29972)
                     /locus_tag="IW651_000880"
     CDS             complement(29193..29972)
                     /locus_tag="IW651_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0813627.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(30179..31390)
                     /locus_tag="IW651_000881"
     CDS             complement(30179..31390)
                     /locus_tag="IW651_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813628.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(31383..32120)
                     /locus_tag="IW651_000882"
     CDS             complement(31383..32120)
                     /locus_tag="IW651_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813629.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            32258..32692
                     /locus_tag="IW651_000883"
     CDS             32258..32692
                     /locus_tag="IW651_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0813630.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            32685..33029
                     /locus_tag="IW651_000884"
     CDS             32685..33029
                     /locus_tag="IW651_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0813631.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            33209..34219
                     /locus_tag="IW651_000885"
     CDS             33209..34219
                     /locus_tag="IW651_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0813632.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(34288..35232)
                     /locus_tag="IW651_000886"
     CDS             complement(34288..35232)
                     /locus_tag="IW651_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0813633.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(35216..37936)
                     /locus_tag="IW651_000887"
     CDS             complement(35216..37936)
                     /locus_tag="IW651_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0813634.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(37933..39153)
                     /locus_tag="IW651_000888"
     CDS             complement(37933..39153)
                     /locus_tag="IW651_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0813635.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(39434..40279)
                     /locus_tag="IW651_000889"
     CDS             complement(39434..40279)
                     /locus_tag="IW651_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0813636.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        12021 a         8437 c         7086 g        13064 t
ORIGIN      
        1 agctatcatt ctgtcacgtt tgtctcactg ctgttcgtac ttgaacttcc tcctagcaag
       61 gcttcgctga ctaagtgaat gtaatgtgtc atcccttcag gatttggatg gacttgatca
      121 tcataaaacc agtcactatg gtcattactg tagctgtacc aatcaatcag cgtcatattg
      181 tcgtattttt tcgtcatctg ttcaagcatc gcattcacgt cattttgcca gcgcttcgtc
      241 ggtactcgga cattgaccca gtatactttt cgatcaccga ttgcatccat cacactatca
      301 aactgagctt ccgagaatga gccattcgtt cctagtccga tcagaacagt gtctttcaac
      361 aaatcttgtt cctttaattt ttcaatgtac ggtacactgg catataattg tcgtccgaca
      421 tctccatcta ccactacttt tggataaagt tcttgtagat ccgttgcagc atccaacata
      481 actgaatcac caaatgccgt tagttctaat ttttgtgcct tattcccttg tgccttggac
      541 aactcgtact tatcaaggat cgcttggtca accgaactag tggtgttttc tgaattgttt
      601 ctggtttctt ctgctaattt tttactttca gcgatttttg cctgcagttg tttttgatcg
      661 gctgttacag cgtttttcgg tgccgtaacg aaaccaacga gtgcaattgc tacaagtaat
      721 gtgcctggaa caacccatgg tttgctctta ctgatgatcg gtttgctgaa ccatcctttc
      781 atcacttgga atgtacgacc ataatcaaaa cgtgctagcg gacgctccac atatcgataa
      841 gaaagttcac tgatgattag aatcaatgtg atttccacta aagtatgcaa caaaacatga
      901 tcggcaatat cagtcacctt tgcttcataa aaaatcataa ctggaaactg atacagataa
      961 atgccatagc ttcgtttgcc aatccaagta aatactggat ttgtcagcca tcgattcaaa
     1021 cttgctccgg ggtgtgctgt cactgcaact agcactacgc ataacagact cactaggagc
     1081 attcctccgt aatagatgaa attgtaatga tcatctaaaa agaagaagca aagaatcagt
     1141 gccaaaagcg agattcctcc agcgatattc agaatatttt tcgcttgttt cgggatgttt
     1201 ttctttaaac gcgtacttgg ccaaacaaac gcaagcgcac ttcccaacca tatagaaaac
     1261 agacgagtgt cggtaccgta ataaacacgg gttggatcgc ctcctggtgt atacaagatc
     1321 atcatcaaga tggcggagag gaaggatgta cctaacacag tataaaagat ccattttctt
     1381 ttacgaacaa ataccatcaa caatacaaac aagatcggcc aaaccaaata gttttgacct
     1441 tctactgcta aagaccagat atgcgtaaac ggtgactcat tagcaaatcg atcgaaataa
     1501 gataaaccgt tattgatttg ccaccagttg ttgacataca gtaagctgct gaccacaact
     1561 cctcgaagat tgtttaacag ccctcgctga aacaaagtga tataagccga agctgtcaca
     1621 agcataaaaa caagtgccgg atagagtctc ttcattcttc tgacataaaa ttgccaaata
     1681 ttgatttttc cattttgttc ccattcctgc cgcagcagat ccgtgatcaa ataaccagat
     1741 acgacgaaaa aaatcggaac gcccaaatag ccgcctttca tactagttgg cagaaggtga
     1801 tagaagataa cacccaccac agctaatgaa cggagcccat caaacccagt gatgtagcgg
     1861 ctgtttttta accttttttg tttttccatt caatattcca acttttcata aacttttttc
     1921 attcgtaata tacgggtatt aagtgaaaat agcaagtaga atcgtgtcct ttctctacta
     1981 tttttttcta tcatcccata aatttaaaaa aataaacatg ctttttttca actttaagaa
     2041 aatttcaaat aaaaaagcag tttttctttg ctgattctac tatggtagaa aaaaactgca
     2101 gacaaacgaa cgtctgtcta cagcagtatt tcatgaattt ttgtcaaact cgatctaaat
     2161 gccatcctca tagaaacgac caagtatctg gacgttttct gcaatactta caaaagcttg
     2221 tggatcagaa gctttcattg cttctctaag tagaggcagt tcgtatcgtg taacgatcgt
     2281 catcaatact gtctgcttat catgcttata agcaccttct gcttcattga tgatcgtgat
     2341 ccctctacgc atacagtttt ggatttcatt tactactttt tctggatgtt tcgttacgat
     2401 cattacctgc atcttctttt gtttagtata aacgacatca gttacttttc cactgacgaa
     2461 aatagacaac gtactgtaaa acatatattg ccagccaaat agaattccgg caacaaaaac
     2521 gatcaatgca ttgaagtaga tcgaaataga accaatcgac tttcctgttt ttttacgaat
     2581 cgtgatactc acgatatcta gaccgcccga tgataggccg tttctcaacg ctagcccaat
     2641 acctagtccc atcacagccc ccccaaacaa cgcacagatg attggatcgg tagataggac
     2701 tgtttccggt aaaatatgga tacaaaggga ggtcaaagtg actgtgataa aagtgaaaat
     2761 tgtaaacttc ttcccgattt ttaaccacgc caatacaaac aaaggtatat tcagtaaata
     2821 caaagttgtt gataccggta cttcgaatcc tatcagtgat tttgacaaag tcgtcaaaat
     2881 ctgagctaga ccagtaatac cacttgaata aatatgtcct ggctgataaa agaaattcaa
     2941 tgcgactgat gcgatcaacg cataaacgat agataccgaa agcttggtcg tgtaatcatg
     3001 ataagcccaa ttttcgaatg tttttttcat ggaaaagccc cgctttatat gttatggctt
     3061 cattataaag tgtttttcaa ttaaatcaag gttgtcaaaa gaagcgtatt cgacaaatcc
     3121 tttttacctt tttatcggct attcttctat attcgttaca tcaatattca attcgaacaa
     3181 ttgtaaaggc gaaaccatac ttggtgcatc actcatcaca tctgctgctt ttccgttttt
     3241 aggaaaggca atgacttcac gaatattatc ttctccggct aataacattg caaaacggtc
     3301 caatcctaat gcaataccgc catgtggagg gaatccgtaa tctaaagctg ttaacaagaa
     3361 accaaattgt tcttccattt gttcttttgt aaagcctaga gtttctagta ctttttcttg
     3421 aagctcacga gtatgaatac ggatagaacc tccgcctagt tcataaccgt tcaagacgat
     3481 atcataagct tctgcataga cttttgcagg gtcttcagcc aataacggga tatcttcttc
     3541 ttttggcatc gtgaatggat ggtgagcaga gacatatcga ccttcttctt ctgaaaattc
     3601 aaattgtggc caatcagtta cccataagaa attgaatttc gattcatcaa ttagtcctaa
     3661 ttcttttcct aaacgtgtcc ggatagcacc taatgcagca gcgacgattt cacttttatc
     3721 tgcgccaaac atcagcaagt cacctggttt tgcatctgtt gctttaatga ttgcttctgt
     3781 tgcttctccc atgaatttag caattggacc tttcaatccg tcttcttcta ctttcagcca
     3841 tgcaagcccc ttagctccga attgtcctac atattgacct aactgatcca tgtcttttcg
     3901 tgagtagcga tctgcagcgc ctttagcatt caatgctttc acgacaccgc cgttttcaag
     3961 agccatttgg aagactttga attctacttc tttgactgta tcgcttagat cgatcaattc
     4021 catatcaaaa cgtgtatccg gtttgtcgct gccgtagcga gccattgctt catcataggt
     4081 catacgagga aatggcaatg tgacttcgat accgcgaact tctttcatta ccttcgcaat
     4141 caatccctct gtgtaagttt ggatttcttc tgctgttaag aacgttgttt caatatctac
     4201 ttgcgtaaat tcaggctgac ggtctccacg taaatcttca tcgcggaaac aacgaacgat
     4261 ttgataataa cggtcgaatc cagcattcat cagcaattgt ttaaacaatt gaggagattg
     4321 cggcaacgca tagaaatgtc cagcatggac acgtgaagga actaggtagt ctcttgcgcc
     4381 ttctggtgta gatttcccta ggtaaggtgt ttcgatatcg agaaagtcat tatcatctaa
     4441 ataatgacga atcgcttttg ttgtcgcatt tcttaatttg atgtttttag tcatttttgg
     4501 acgacgcaga tccaaataac gatatttcaa acgtatttcg tcactgattg cttgatcatc
     4561 ttcaatttga aatggcgggg tttttgcttg attcaaaatc acgatttcac ttgcaatcac
     4621 ttcgaattca ccagtggcca ttttaggatt tactgcattt tctccgcgtc ttctaacttc
     4681 cccagtcact tcgatcacgt attcactgcg acatttatcc gcgatttccc atgcttcttt
     4741 gttgatttca ggattaaaga cgatttgaac gattccttca cgatcacgta ggtcgataaa
     4801 gattactccc ccaaggtctc gtcttttttg tacccatcct tttaatgtaa ctgtttgttc
     4861 taattgttgt tcggaaacta atccgcaata gatcgttctt tgtgccattt atttttttcc
     4921 tcctactcga ataaagtcat ttcatcatag acttcatcaa aatgttcata tatctcttct
     4981 aatggaaaac ttttttcttt tcggcttgcc attgctttga cattaatcgt ttgactagca
     5041 agctcatctt caccgatcac caatactaat ttggcattcg ctttatctgc tgtcttaaat
     5101 tgcgcttttg ctttacggtt catgaagtcg cggtcagctg aaaaaccaaa gtttcgaatc
     5161 gcttggacca ctttcaatgc ttcgatattt gtttcgtctc cgagtgcgac cacgtaagca
     5221 tccaactcat taaatgcagg gataactact tcctctgcct ccatagtcaa gagtaatcgc
     5281 tcgataccca ttgcaaatcc gaagccaggt gttgaaggac cacctagttc ttccactaga
     5341 ttgtcataac gtccgcctgc gcaaatcgtt gcttgtgccc ccatttttgg tgcgtcactc
     5401 atgatttcaa aaatcgtatg tgtgtaataa tctagtccgc gaaccatatt actgtcgatt
     5461 tcataaggaa cagccaatgc atccagcatg gaaacaacgg tatcaaaatg tttttgagca
     5521 ggctcgctta gataatctaa gatagatgga gcttctgcca caaatttttg atctcgtttg
     5581 tctttactgt ccaatacgcg taatggattt tcatgaaggc ggcgtttaga gtcttcactc
     5641 agctctgatt caaaaggtaa aagatagtcg atcaatgctt gacgataggc ttgacgcgtt
     5701 tctttatcac ctaatgaatt gatgactagc cggatttgcg tgatccccaa ctgtttgaag
     5761 aaatcaagtg ccatcaccat gctctccaca tcagttgcag ggttttctga tccaaatgct
     5821 tctactccga tttgatggaa ttgacgtaac cgtccttttt gagggcgttc gtagcgaaac
     5881 attggtccca tataaaaagt tttgaacggt ttcgcatact ctgggccgaa caatttatgc
     5941 tcaacaaaag aacgaacgat tggtgctgta ccttctggtc gaagcgtcac atggcgttcc
     6001 cctttgtcat aaaaatcata catctctttt gaaacgatat ccgttgtatc ccctacactt
     6061 cgggatatca cttcatagtg ttcaaaaatc ggtgtacgca tttcattata ctgataatct
     6121 ctgaataata aacgtgctgt ttcttcgaca aactgccatt tttctgattc gcctggcaaa
     6181 atatcatttg ttccttttgg tctttggaaa ctcatagttt tctctccctt tcttagctat
     6241 gtaaataaaa aaatcaccct tgttcatcat gaacaagggt gaatgaaatt catacacggt
     6301 accacctaat tttgaaagat ttctctttcc tcttacgaat aacgctcgta cgcgggacgg
     6361 cttttcactc gtccatctcc agagtgtctt taggctaatg aaactttaga tcattttcag
     6421 cctcgatgat cctctcttgt aaagtttcaa aaacttactc gctctttcat tgacattcga
     6481 tattacttat aacctactaa aaaccagtct caaagtcaag agatatctga ctatttcccc
     6541 tcacaaaaac gttttagttc agctttttgg tattttgttt tagactagac aaaaaggagg
     6601 aatcagctat ggcagaaata agaatcggac attcacaagt ctacgcagaa cagcttggat
     6661 taggagcaaa tgccgttgga ggacataatc tttttgacgg tttggaagat gagacaggaa
     6721 aacaagtagt tcgtacagct ttgaatagcg ggatcaattt gatcgatacc gcttatgctt
     6781 acggaaacgg ccgttccgaa gaactgattg gcgaggtact gaaggaaaaa gaatatgatc
     6841 gctcacgtgt cgtcatcgct acaaaagctg ctcatgtacc aaataaaggg cgaacatttg
     6901 ataattctcc tgagtttctc aaacaatcgg tggaagatgc actaaaacgt ctgcaaactg
     6961 attacatcga cattttttat atccactttc cggatgaaag cacaccaaaa aatgaatcag
     7021 ttgctacatt gcacgaatta aaagaagcag gaaaaatccg agcagtcggt gtgtcaaact
     7081 tcacgttaga acaattgaaa gaagcgaatg cagatggata tgtcgatgta gtagaagata
     7141 aatacagttt gatccatcgg caggctgaaa aagaattatt cccttatctt gaaaaaaaca
     7201 aaatcagttt tgttccctat tttcctcttg cttccggttt gctgacagga aaatatgaac
     7261 ttggtgagga aaaacaattt ggtgaaggag atcctcgaaa aagaaatcca gatttccaag
     7321 gagaacgttt cagagaaatt ttaaccgctg tcgatgtact tcgccccatt gctaaaagat
     7381 accaagccac tcctgcgcaa cttgttttag cttggtacat gaagaatcca cgagtgtctg
     7441 tagttatacc tggagctaag cgtccagagc aagtatctga caatgtccaa gctttagatt
     7501 tgcatttatc caatgaagat tatcaaacca ttgatgaagc atttcgtgga ttttgatcaa
     7561 ttgaccttat caatttatag taatatgaaa aaaggaagat cagcactgtt tgatcttcct
     7621 ttttttcgat aagtttatgc taatctaatt cttgtaaagt agcgctcggc tattcggata
     7681 agtcggaaac tgattgaatg gaatcgtcca atcttgttca ggtacagaga actcaaactt
     7741 atttactagc tgatcggaaa acacacgtag actatgaaga gtgatccatt ctcctgcaca
     7801 gcgatgcatt tgtcgaaaat ttcccccgcc ttgtgcaatc atctcatatt cttctttata
     7861 ggaaatatct tttgctttcc ctacataccg tttgatcata aaggaatctg gcgcttcaac
     7921 tgttcgttca tcatggtctg ttccatataa atctaaaata acccaactgc cttctggtat
     7981 acgataacca tctacttcta cttctttcag cgaaatcgca ggtagcattg ggaagaaagg
     8041 ataatagcgt ctcatttctt gaataaacgg atcttgtaac gtcgaaaagt cattttttaa
     8101 ttgctgatac aaatctggtc tactgaaaag tgcatgtccc atcaatgccg cccacacagt
     8161 tagagcaacc gttggacgga tgatgttcaa taattcaaca gctgccactt ccagaggtaa
     8221 aagctgacca tctaaatctg tggcattggc aaaagcatat aatgccacat tttcttttcc
     8281 agccacagga ttttgtcgag cttcttcaat caagccttgc gcccattttt ctgatttttt
     8341 gcgattttcg acacctttta tgtgatcaat cggagaagta aacgttccgc tgatcataga
     8401 gatttgatat tctgctaatt tttcgacctc atctttagaa atcgctgata aattgatacc
     8461 tgcccactcg caaatagatg taaagaaaac catcttactt aaatcaaata gttcaaattg
     8521 accatgctgt gcttcaagtg cttgtgtcag attcttatct aagatacgat gataatcttc
     8581 cattctttcc ggtgtcatta gatccataaa aatatttttc cgatggtgat gagcagctcc
     8641 atccaaagtc tgtactccac cttgaccaaa aagtgtcttc aatactaatt ttggcatagc
     8701 accttttcgt ttgaaattcc ttggatcata aaatactttt gctgctgatg atccgtagat
     8761 cgtgatagct tctttattga aaatctttgc tttgacgacg ggagcgtccg cttcatggcg
     8821 taactcctcc aacatgttgt atcctttttg atacaatttt tttaaatctg tgattttgat
     8881 atctactact ggtacttctt tcatcttcag caacctccta taaaaaataa cttgtataat
     8941 ttaagcttaa ctttttttcc gactgctgac aaaagaagcg ctttttgcag acaaaaaagc
     9001 cacttaagtt agagtttatc agtctaactt aagcgacgtg tttcatagtt tttactagtg
     9061 aaaaccagat tactttattt gtttttagaa tcaataatga tcgttacagg tccatcattg
     9121 acgagagaaa ccgacatatc tgctccgaat tgtccggtag cgactgtgat gccgcgactt
     9181 ctgattcctt cattgaatga ttcgtataaa ggaatagctg tttctggtcg tgcggcttcc
     9241 acaaagcttg gtcgatttcc ttttttcgta tccgcaaaca gtgtgaattg agagatactt
     9301 aaaatttgtc ctcccacatc ttctacacta atattcattt ttccttgttc atcttcaaaa
     9361 atacgcattt gtgcaatttt ttttaccaga tagtccacat ctttttgcgt atcggtttca
     9421 tgaatgccta ataaaacaag taggccgtag ccaatttttc caatttcttg ctgatcgact
     9481 gacacagcag ctttacttac ccgttgaaca actgctctca tatctgtcct cctatccatt
     9541 cgtccggcgt acactgtaga catcaggtac tgctttgatt ttatccacga tactctttag
     9601 gtgggataaa ttctggatac caaccgtcaa atggatcgtc gccattttat ctttattcgt
     9661 ccgtgcttca acgctgttga gatttttcgt tagcgcattg actgtttgaa gtacatcatt
     9721 caataagcct gaacgattat agccgtaaat ttccaaatct gcatcatact cttttcgtgt
     9781 attcgatgta tcttcccatt cgacttcaat caaacgttct gctacatttg gtttgtcagg
     9841 ctgaacattc ggacaatctc gacgatgaat ggatatccct ctgcctttag tgatataacc
     9901 aacgatatca tcaccaggaa tcggattaca gcagcgactg atacgaatta gtaagttttc
     9961 taccccttga atgacaacgc caccttcatg acgtactttc attttgtcag gttctttttt
    10021 cggctgattc atgatttctt ctgcttcttg cttttgctgc tcgatttttt gttctttacg
    10081 ttctttttca gtcagacgat tggccatcgt caaggggcta acttctccat accctacagc
    10141 tgcatagaga tcatcttctg tttgataatt aaaacgatcg agtgcttctt gcagtttgtt
    10201 cttcgtcaaa atatctttag gcgtaaatcc tagatctgta atacatttga ccacggattc
    10261 gtggcctttg atcacatttt cttctcgatc ttgggctttg aagaaacgtt tgatcttatt
    10321 tcttgctttg ctagtagcaa ctaatttcaa ccagtcgcga cttgggccaa atgaatttgg
    10381 agaagtcatg atctcaatga tatctccgtt tttcaatttg taatcaagct gcaccatttt
    10441 gccatttact tttgcaccag tggttttgtt accgatatct gtatggatac tgtatgcaaa
    10501 gtccaatggt ccggatcctt ttggtaactc agtaacgtct ccttttggtg tgaagacgta
    10561 gactttatca ctaaagatat ctccttttac gccttccata aattcagaag catcatagct
    10621 ttcgtcttga agttcgagta tctcatggaa ccagcttaat tgtttcgtca taccatctgg
    10681 ttctactttt tcattttttc cttctttata ggcccagtgt gcagcaaccc cgaattcagc
    10741 gatttcatgc atttcctgtg ttcggatttg aatttctacc ggatttcctg ccggaccgat
    10801 cacggtagta tgcaaagatt ggtacatatt agcttttggc atggcaatat aatccttaaa
    10861 tctgccgggc attggtttcc attttgtgtg gattgctcct aatacagcat aacaatcctt
    10921 gattgaatct acgatcactc gaatagctag caagtcataa atttcgttga actgcttttt
    10981 ctgatctttc attttacgat agattgaata aatgtgtttt ggccgtccat agatttctgc
    11041 aaaaatcccc agctcttctg tcgctattcg aatatcttca acagtaccac ttacgtattt
    11101 ttcacgttct tctctcttcg tctgcatcaa gtggacgata cggtaatact gttttggatt
    11161 tagataacga agtgctgtat cttctaattc ccatttgatc cggctgatcc ctagacgatg
    11221 tgcaagaggc gcatagattt ctaaagtttc ctgagcgatc cttctttgct tatcttctcg
    11281 caggtgcttc aacgtacgca tgttatgtag tctatcagct aatttgacca tgataactcg
    11341 taaatcttga gccattgcta gcagcatctt tcggtggttt tctgctagct gctcttcgtg
    11401 ggatttatat tttattttcc ctaatttggt tacgccgtcc actaacatag caacgtcatc
    11461 gccaaattct tctctcagat cttctaaagt gatttctgta tcttcaacaa catcatgtaa
    11521 gaagcctgta gccactgtat ggggatccat ttttaaatct gctaatatac ctgcgacttg
    11581 gataggatga ataatgtagg gttcgcctga ttttctaaac tgtccgtcat gtgcagcagt
    11641 cgcgtattcg catgctttct caacaaatgc tacatgttcg ggacccatgt attgactaac
    11701 gagctttatg acaccgggtc ccgttaaaat ttcttctttt gtcacaggaa tcgatccccc
    11761 tttctttttt cggtaaaacg ggaatcggga caaatatctg tttgataatt gtcccaaaat
    11821 ccgtgcagtt atacgttttt ttacggaaat gttcgctttt taaaagcttt ttcaaacgat
    11881 ttttcgtatc cattgtataa ataattgttt ctattataca aattgttgac tattttttca
    11941 attctttctg tcgatactct tttattaatg aagaacgtta gctgaaagaa ggattcatta
    12001 attcccattg atagctagca gcacttaata aataaagagg tgctgtttct gtacgaagga
    12061 ttcttggtcc taatccacaa agtatccctt tgtgttctac aaaataatcg acttcatcgc
    12121 gggataatcc accttctggt ccaaagacag ctaagattcg actatctggt tttgtttcac
    12181 taagaatttt ggctgcattt gatttttcgc cttgctttgc tgattcctca taagcaacca
    12241 agaccacgtc ataatcatct agccgctcaa ataaatcttg tttcgtttcc aataactcta
    12301 ctgatggcac gatctgccga tgggattgtt ccgctgcctc ttgagcaatc ttttcaagcc
    12361 gttgttgtcg tttcatacgt ttttttgcat cccatttaac gacagaggct gatgccggga
    12421 aaccaagaaa agcttgcgct cctaattcag tggatttctg aacgatccaa tccagctttt
    12481 cccctttagg atagccactc gctatggtaa tcgaacttgg cagttccttt tgattttctt
    12541 cttttttaat ttctttcaga taaacagcct cttcagtaat ctccgtgatt tctgcaacaa
    12601 tggctatttg atcattgaat gccaaaaaaa cttcatcctc gggttccatc ctcataaccc
    12661 gtacaatgtg gtgatagttt tccccttcta aaacaaaata gtcttttgat tcatatgatt
    12721 cttttaaaaa ataccgctgc atctattcca cctctggttt tttcaaaata attgcatacc
    12781 agtctttttg ctggaaaatt tgatccacga taaacccttg ttctaacatg gcatccagaa
    12841 ccatttgctt cttctcttca atgattcctg aaacgatcag cgtgcctgtt tgcttcagta
    12901 aacgccaagc atctgggatc atcaataaga tgatatctgc taaaatgttc gcaacgatta
    12961 catcactttc aatctcgatt cctgtcaaaa gatcgttagc tgatacatgg acatctttgg
    13021 ctatttcatt catatccata ttttctttcg ccgaacgaac ggctacttcg tctaaatcaa
    13081 aggcatagac atcttttgca ccaagatatt ttgctgcaat acttagcacg cccgacccag
    13141 ttcccacatc tagcaatgtt tcaccaccac gcaagactgt ttctagtgct gtcaatgtca
    13201 aggaagtcgt gggatgagtg cctgtgccaa atgccatacc aggatctagt gtgataattt
    13261 tttctagagg atcttctgtc tggtagtttt cccagcttgg cgcaaccgtc agcattcgcg
    13321 taatacgcac cggatgatag tatttcttcc aggctgttgc ccagttgctt tcctctactt
    13381 cactaactgt tactttattt tttccaatag ctagaccaaa ctcaggtaat tgtgtgatac
    13441 gatctttgat aaaaggcaag atttccggta agaaagtcgt ttccggaaaa taagccatga
    13501 ctagtgcgcc ttctttcagc gatgtaaaat catttttatc taaaatctca ccatattgat
    13561 ccgactgaaa atgttcaaca tctaaagaat cttcaatcgc gacaccactc gcccctgcct
    13621 ccatcaaaat attagcaact gcttcgactg cttcactttc agtttctact tttacttcat
    13681 tccatttcat atctgtcact ccttaataac ttgggtacgg taaataagta tctgcttcat
    13741 tttgctgttt tacttctact aattctgcta gttgctgatt atcccagtga agctcgaata
    13801 cagcttcgtc atcgtcttca tccaaaaaat caagcaaatc gctttgccct tgagctaata
    13861 cttcatttag atattccact aaagaatcag ctactgattt tgccaaccct ttttttcctt
    13921 cataagggat tactgctaga taattgcgtt catcaaaaac agatttctga ggattaaaaa
    13981 gaagtacgcc gtcttcaaat tcgataattg gttcttgcga aacgatccct tctgcatcat
    14041 caatctcaaa ttgtgcttta ttttctgcaa acaagcgaac aacgatctcg atcgtatgat
    14101 tttttgtatc ccaatctaaa gctacatcat agtctgtcac ttgtttttca attatttcat
    14161 ctaaatatgt taatagatcc ttctttttca ttttctttcc tccaacccca atctttctcg
    14221 atttggtttt ttctttgtaa agagatataa ggatttcccg acacaacaaa acgcaacggc
    14281 ttttctgtcc atacaccttt gtttgggata ccgattctag gcaaagcatt gatctttttt
    14341 ggtgtctttt ttttctcaaa aactaagcgt aacgaactat ctgctataga ctgaccataa
    14401 agttcttgag gaattgctaa tgcttccact aattttcccg gaccgttgct gatatctgga
    14461 ccagtttttc cgccacgatt tttactcatt tgatcaatca ttgcggcggg ttcgattgca
    14521 cgtatcatta ctccttgagg aatcccttct ggctgagtaa tgatgttcaa aatccgatga
    14581 gtatgcatcg tgtaaaggta gatcgtacct ggtttttcat acattgcccg cacacgcggt
    14641 gttcttctta aaccatagct atgggcggcc tcgtcttctg gacctaggta tgcttccgcg
    14701 tctacaatat atccagctaa tcttccttct ggtgtgtcat gctccaaata catccccaat
    14761 aaatcccgtg ccacttctgg cgtcgtttta ttccaaaaaa attcatctat cttttgttta
    14821 tccgtcattt ttctcaccta ctttatcata cacaatatta gttggataag ctatgaaaac
    14881 aaaacaacag aaggctgttc tttctaggca tcttgctaaa tatgatataa ttaagggacg
    14941 taaaaacaag aaggaggaaa atcctatgca acagccattg gcttatcgca tgagacctcg
    15001 aacgatcgat gaagtcgttg gtcagcagca tctagtcggg gaaggaaaaa tcatacgccg
    15061 catggtcgat gctcgcatgc tctcttccat gattctatat ggacctcctg gtactggaaa
    15121 aacgagtatc gcaagtgcga tcgccggctc tactaattat gcatttcgca tgctaaacgc
    15181 cgctacagat tcaaaaaagg atctacaagt tgtagcggaa gaagcaaaaa tgagtggtac
    15241 agtgatcctc ttattggatg aagtacaccg attagataaa acaaaacagg attttctgct
    15301 tcctcattta gaaagtggcc gaatcatctt gatcggagcg actactgaga atccttatat
    15361 taccatcaat ccagcgatcc gcagccgtac acagatcttt gaagtcaaac cattaacaga
    15421 acaagacatc cagttggcag tcgaacatgc tttaagggat aaagaacgcg gccttggaca
    15481 acaagcaatc cagttagacg aagatgctct tcttcacctt tcgcgtgcaa cgaacggcga
    15541 tttgcggagt gcattgaatg ggctggaact ggctacacta tcgactcctt cagataaaga
    15601 aggaaggatt catcttacac tgtcgatcat tgaagaatgt gtccaaagaa aagctcttac
    15661 ccatgacaaa aatggagatg ctcattatga cgttatttcg gcttttcaaa aatcgattcg
    15721 tggaagtgat gtggatgctg cacttcatta tctcgcccgt ttagttgagg caggtgatct
    15781 ggcaagtatt tgccggcgtc tgatggttat cggttatgaa gatataggtt taggtaatcc
    15841 tgctgctgcg gctcgtacag tgaacgctgt attagctgct gaaaaattgg gattgcctga
    15901 ggcacgtatc ccactagcag acgtcgtcgt cgatctttgc ttgtcgccaa aatccaattc
    15961 tgcctacatg gcattagatg cagcacttgc tgatattcga gagggcaaag ctggagatgt
    16021 tcctgatcat ttgagagata gccattacaa aggtgcgaaa tcactcaacc gtggcgtggg
    16081 ttatcagtat cctcatcatt ttgatcaggc ttgggtcaat cagcaatatt taccagataa
    16141 actgaagaat gctcagtatt atcagccaaa agatacaggg aaatatgaac aggctctggg
    16201 tcaacagtat taccgtataa aagagtggaa aaagcatcct ccaaaaaact agacaagaaa
    16261 acggttctgt ttctgcgagc tctctcattg ctctttttaa attcatatgc tactatagag
    16321 gtgggaaatc tgtgatgttc gaattgtcct ctttgtgtgt tgaccgaaca tttttattga
    16381 tatcttggga cccgtcttgt gtcactgctg gtaacatgcc ttatcatttg gtagttcgaa
    16441 gctacgacca tgggacccac ctgcttgaat tcgcgggatc aatactacag gacaaacaac
    16501 ggcattaacg gagattacca tcttttaaag aataaagcaa tttgctcttg gcaaattgct
    16561 ttttctattt gttgcttatt ttggaggatt tgttcatgat taggatttta atgattatcg
    16621 ccactttagt attattattc gttagctatt atttatttaa taaacaggat atcttttttg
    16681 tattgatcaa caaaaatgat aaaaatcaag gattcctaca attttacgga gctgcatatg
    16741 ctgttttagg cgtaatgggt attttaacag cttttttcaa tcaacgcttt attgccttgg
    16801 tctttttact gatcgtaatt attgtttctg ccaccttcag tatccgattt gcaaaaaaaa
    16861 ttgctgaacc aaagcagtaa ataattctct ttttccctaa agtgttctac aataaaagag
    16921 tacaatacga ttgacttgag gagtgaatgc ttatgttaca acaatacaaa aaaatcatgg
    16981 ttgccgtaga tggttctgat gaagctgaat tagcttttaa aaaggcggtc aatgtagcta
    17041 tccgaaataa cggagaactt ctattagcac atgtcattga cacacgttct ttccaaaccg
    17101 tttcatcctt tgatggaatg cttgcagaac aagctactga aatggcaaag caaacattgg
    17161 cagattatga aagcaatgcg aagaaagctg gcttgaataa tgtaacctct gtcgtagaat
    17221 atggctcacc aaaacaaatt attgccagag aaatcccaga agataatcaa gtagatttga
    17281 ttatgttagg agccacagga ttgaatgctg tagaacgttt atttattggt tctgtttctg
    17341 aatatgttat tagaaacgca gcttgcgatg tgcttgttgt ccgaactgat ttagaaaacc
    17401 aattgccagc taaagaccac accaaagctt aacacgtaga cgacctgcca actatcggca
    17461 ggtttttttg cacaaaaaac cgtgataaaa acaaaagacg cggcaagcac tcatcggtct
    17521 ttataatgtt taaaataaag ggaaaacagt caatgagttc aagtaaaata aaaaaaagtg
    17581 aacaccaggg cgattagtcg ccctggtgtg gtttaaaacg acctaagcat gccaaagctt
    17641 attggttcgt caatgtggaa gtttccgatg aatctgatac gaaaaaatat gaagtatcga
    17701 tggcggatac cgattttatc gccaaactaa aattttattt cgggttcggg taccgcgaga
    17761 aggcaagtgc tacattcggt aaatcattca agaattgatg aaaggctacc gcttgccatt
    17821 gcaaccacaa cgatactcct gaacgcgatg ttggtgagat ttccaaagag aagagctgtt
    17881 taatgcaacg gtattaagca aatggcaaaa ggaagtcatg tagacaaagt tttttatcaa
    17941 acaccatatg atttcgcctg aacgctggtt tagctcagaa tacaatgatg tggtccacga
    18001 gacattaccc tcagtcagat aaaaaaagct ctctgacaga attacacatc gataaatatt
    18061 aagtaaatgt cagcgctagt tgaactttct gacactttca ggtacactgg aaaagacaac
    18121 tacacgaagg agggaaaata ctatgatgcg catgactaaa ataacaagta atgaaaatta
    18181 ccaacgatac catatcgcat ttagccatgc aattggtttt acagttgctg ttgacgggac
    18241 aagtttgtcc aaaacagttg aattcacgta aaatcaatgg ccatctcaag gtatttttct
    18301 catgataatc tgagtttaaa taacacaggc catggataac catggccttt ttgagtggtc
    18361 tcaaggagag agatcatgat catacaagca atttctatta aaaaaacaat cggcgggaat
    18421 ccattatttg aggatttatc ctgtcaaatt gaagctggtg aaaaaattgc cttagtcggt
    18481 atcaacggaa ctggcaaatc aaccttttta caaatcttga ccggcagaga aggtgtcgac
    18541 agcggtgtta tcagtcgcaa aaaaggctta aagatcggga cggtcgaaca agaacttaca
    18601 gtttccgaag cgaccgtcaa ccactacttg ctccattctg ccgctgagat acaggacctc
    18661 aaacagcaaa tgagtcgcta cgaagcattg atgacagaac cagatacaaa cttagagaag
    18721 tgtctggccc gttacggtga gctgcagcat cgctttgaag aactaggcgg ttacgtatta
    18781 gaagaccgga ttgcagcgat cttgcaaggc ttagggattc cacaccgtca ggaagcacgg
    18841 ttaacagaac ttagcggagg cgaacgagtt aaagtcgctt tagccaaaat tttagcagcc
    18901 gatgccgatt tacttttact cgatgaaccg accaatcatt tagatcttaa cagtattcgc
    18961 tggttggaaa actatttaca gaatacaaaa aaatcggttc tggtcgtttc tcatgatcgg
    19021 caatttctcg atcaagtcac cacaaaaaca tgggaactgg aagaaggtgc attgattgag
    19081 tatcccggaa actacagccg ttttcgagtc ttgaaagaag ctcgcttggc tgagctgaca
    19141 aaaaattacg aactgcagca aaaagaagtc cagcggttga aagtcatgat tcgtcgtttt
    19201 cgtcagtggg cacatgaagg agacaatgag tccttcttca aaaaagccaa agaactggaa
    19261 cgccgcctgg cgaaactgac actcgtcaaa ccgccacctc ccccgaaaaa tcggctgcaa
    19321 agcttgtcta atggtggaaa atctggcaaa gaagttttca tcattcaaaa cttgcaccag
    19381 cagtacgctg atcaagtact attcaaagac agcagttttg cagtctatcg tggagatcat
    19441 ttagccatca tcggcgacaa tggtgcagga aaatcaacct tactcaaatt attactcggt
    19501 gaggaaaagc cttacggtgg caccatcaaa ttaggtagta gcttgcaaat cggttatttg
    19561 ccgcagcagc tgcagtttgc cgatcccgat agccgtttgc tggcttacgc catcacattg
    19621 actggaaacg aagaagtcgc tcgacggcaa ttagccaaaa gtggctttta tcaaagcgat
    19681 gtgggtaaac ggatcaaaga tttatccggt ggtgaaaaaa tccgtttggc gttgctgaaa
    19741 ctctttttag aaaaaatcaa cgtcctgatt ttggatgaac caaccaatca cctcgacagc
    19801 tatgctcgag aagaaattga agaaatgctg caagactatc aaggaacgct cctagcagtc
    19861 agccatgacc gctacttttt gcagcaacac ttccaagaag cactcgttat tgatcaggag
    19921 cagatcagcc gccagccgct cggccaactg attgactagg acgttttttc gcaacaaaaa
    19981 acttgatttt catacctgtg ctttcacgat tcaggcaaag tacaggtgga aaatcaagtt
    20041 tttattattc gatcaatgaa tgtttcggat cggttttcta gctaaacata aagcgaagaa
    20101 taaagtcgac agcccacgag gctgatctaa aaatgttttt tctttttaca tatgtaatgt
    20161 cccttataaa gatgaatgaa aataaaatat tgggattttc ctgaaaatca ataaataaaa
    20221 tgccagtctt ttcccttttg accacttttc caagcaaaat tccctaagaa gatcctgtaa
    20281 aaaagaaggg aaattgaagt taaattacca ttttgcggtc ttctccgcaa aaaaaacaca
    20341 tgcaagaatt ccctcttgca tgcgtttcat cttttatcct aacgaatgct ttggcttagc
    20401 cgcgcagacg ctccacatca cgggcaatca ttagttcctc atctgttggg atcactaata
    20461 ctcttacttt tgattctttt gtagagatat ctacttcttc accacgcaat tcattcaacg
    20521 ctggatcgat ttcacaacca aaccaagaca tacctttgat gatttcacta cgtacatgtc
    20581 cgtcattctc gccgatacca gcagtaaaga caattgcatc aacaccattc atcgttgtca
    20641 cataaccgcc gatgtatttt cggatgcggt ccacgaaaat gtcataagct aaacgaatat
    20701 gttctttttc atagttcttt tcaagatcac gcatatcact agacaatcct gttaggccta
    20761 gcagaccaga tttcttgttc aaaacattga tcatttcatc aatatcaatc tctaattttt
    20821 ccattaaata tgggagaaca gctggatcga tatcacctga acgtgtaccc attgtcaccc
    20881 cagctaatgg tgtgaatccc atagaagtat ccatggattt acctccatcg actgctgtga
    20941 tcgatacacc atttcctaag tggcaagtga tgatttttag ttcttcgatc ggtttgttca
    21001 ataattttgc cgcacgttcc gacacataac gatgacttgt gccatggaag ccatattttc
    21061 ttactttgaa atcttcataa taagacaccg gtaagctata taaataattt tgctttggca
    21121 ttgttgagtg gaaagatgta tcgaatacag ccacactcaa aatgtcaggt aaaatctttt
    21181 taaacgcttt gatccccatt aagtttgcag gattatgcaa aggagcaaat tctgctaatt
    21241 cagcaatgcg gtccattact ttctcatcga tcactactga atccccataa gtttctcctc
    21301 cgtgaacaac acggtgccct actccggtga tttcatcata agagcttaaa atttgcagtt
    21361 caatcaactg atctaacagc atttttactg ctacttcgtg gttttcgata tccatgattt
    21421 gttcgaattt ttgattatcg ccgtacttga tggtaaaaat agaatcttgt aaaccgatac
    21481 gttcgacgat ccctttcgca actacggttt cttctggcat ttgatataat tgccatttca
    21541 aacttgaact cccagcattg attgcaattg ttttagacat atgactctcc ttctcgttga
    21601 atgattacag agcagatgat ttccagtttt taaactcgtt gaaaaattcc gtcaccttct
    21661 ctggttcttt taatgaagca agtttcacaa gcaagacttc ttttacttgt tgtgcttgtg
    21721 gtcctttttt ctgtaagatc aaaatacttt tttgcgattg tttattacgg aacaattcat
    21781 ctggcaattg aatcattcct tgaagataac cttcttcttt aaaccatttt ttgatttctt
    21841 cactttgatc cgtttctaaa aatccactcg gcatcaaaaa caaaccaaaa ccatccggct
    21901 tcacatattt cattgattgc tctagcaata aatgatgtgc ataactatgg ccttcttcgg
    21961 tacttgttaa aaactctttt gctttttcgt cattcggata ataaccgatt ggcaaatcgc
    22021 tgattgcaaa atcgacagga tctattaata attcctgcaa cccgtcttga tgaaagtatt
    22081 gaacatttgc ttgtgtcaaa tcacttgtag aagctgcgac agccaacaat gtatcatcaa
    22141 tatccacacc aattccttct gtttggtacc ccgcattcga taggtttaaa agaactgtta
    22201 ataacaagtt gcccattcct gccgcaatat ccagaatctt aaccggtgct tttttatttg
    22261 tgtatagttg ttcaataaga aaaacaaata aaaaaccgat actatcagga gtgagttgat
    22321 ggttcgcctg caaatgttct gtctggctgc ccttcaataa aagtaattga gacagtctgc
    22381 gccattcttc tggctcgaaa gaaagtttct tcaactcttc ataaagagct gtgatccgtt
    22441 gcgttgtctc ttttgtcggc actccatcca ccacacgtac ttgataatca tctattaaat
    22501 tttctgcatt ttcgacatag gcttctaaaa aggaagttcc caatgcattc tgtaaaagct
    22561 gaatagcttc caggttttga ttaaaccctt gttccatttt ttctggtaac atggtgcacc
    22621 tcatttctag atatattata acaatttttt cacctgttta attctatcga acaattgaca
    22681 aaatttcaat cgttttcact gttttttatt tgtgaaacgg caaacatttt tttattaaaa
    22741 acagtgttat aacagaaaag agggcgtttt cacgaacatt ccatcattct gtactagtat
    22801 ctgtatcatt ttttacttct tctgtgaacc gaaagataaa cggtgagact tccacctcta
    22861 agaataggga ctgttgtacc cactgatatg ttacagatcc agttgtgtag taaaaaactc
    22921 cttccgaact ttcggcaagc gtagactgtt ccgataaagc catctgcttc attatttttg
    22981 ctatataata atttttgatt cgtatattaa gatccattga caaacggtag ctttctaatg
    23041 ctgccatcaa taaaaacgta cataaaagaa ataaaagcaa gacagaaaat aaaatagagc
    23101 ccttataaca ttttctactg atcgtttttt gtccatatgg catatccatc ttctccattt
    23161 tcaaagtgta cataaaaaga aattgtgtgt tctttttcca ataattgaag ctcagtgata
    23221 cttgttaaca taggttgatg tccgccggaa gttcttcttt tacgtatcat tgcctgatat
    23281 ctttcgatga tatactgttt atcctcgatc gtgtagtgca gttgctgatt tttcacagag
    23341 acattgtgac tgctcttgag ttcttcttct aattgtgcta aaaatattag ccactctttt
    23401 tgatctttac gctgcaaaaa ttcacttact tggatggttt gctgaatcaa aagattgaat
    23461 gtgagcaata gactgctcaa tatcatcagt ccaattagac attcaatcag cgtaaatgct
    23521 ggaattactt gttttttcaa tactgatcgc ttctttctcc atattgacaa acactttttc
    23581 gattactcca ttattatcga caatttggat atcgtatggg atcatttctt cccttcgctc
    23641 tggtcttttg tgatacatcc gctgcgtctt gatatcttca taaatcattc gaataaatag
    23701 cagttcctcc ccacttttct tttctgcctg aagcaaaaac agctggccaa agctcactaa
    23761 gtagacacag acagaagcga tcataagcgc tgtaagggac tccaacaaaa caaatcctgt
    23821 tttattcaat tctttttgta tagcgaccac tccctatctg gaattgatac gtaatcgtcc
    23881 gctctttttc ttcccaataa aattgataag cttttagtga actttcattc ccggttctag
    23941 cagcaaaagt aatagaagca tggcgtttca aggtaatctc atcaggaata tctaatactt
    24001 cccaatttat tttatctgga ttcggaacat gaaaaatgat ttgattctct tcactattcc
    24061 aataaaagcc tgtttggatc tgattcacaa tagcgatttt ttgcgtagaa tagattcgtt
    24121 tttcaaattg gccgaaaaat tgatagactg ccaattcatt cttccatgca gaaaatgcga
    24181 gaactggaaa agaaaggatc gttgtaagta ataaaatcag taagagtgat tcaaacaacg
    24241 tatatcctga tgctaaaacc ttaccttcaa ggccgctgat agatttctgc atgttctcgt
    24301 ttgatatagc cttcattcaa caattcctcc acagtaggaa ctcttccatt attttgaagc
    24361 gcatagagct ctttttgtgt gtctaccaac tggatgatcg cttcacgact ttcttggtct
    24421 acatgattcc tataacgcga caaattaggg acgaacaaca acactaatac actgatgatc
    24481 agtaagacga ttagcatttc gatcaatgta aaaccgctat aataagatat ttttctcttt
    24541 ttcatggcaa aacctcctct atgcttccgt aaacaggtaa taaaacagcg gcataaacta
    24601 ataaaatcaa agccgctacg cccaaaaaaa tgattggctg aataaaatgc atccagtgat
    24661 gaagacgttt gaaaagctgt tttcttgtcc attcgctgta aaaaatcagt tctttaccta
    24721 aacttccctt tgcctctccc tgtaaaacaa ttttactgaa ttcttctgat agaaaacgat
    24781 aattctggaa ctgctcagcc aatgacatac ctgcctccaa tccttgaatc aacttagagg
    24841 caagatgctg aatcagactt tcttgtttcg tttcctttaa gcagactatg atttgcctta
    24901 attcgaaacc ttcatgaaaa agctttccaa tctctagtga caaataagcc gattgataca
    24961 aggaatatat cggtccaatc aataattgtc tgcttatcca ttcactctga tcaacaggag
    25021 acaacttcat cagacgatac cttactagta aagccaatag agtaaccgct aggatgaata
    25081 gtagaacata ccaatgaaag acttgtatca gctgaatacc ccaatgctct tgcatgacca
    25141 ttcctgaacc aataagctga ggcaaaacga acaaccgcat actaaacaag atacctagca
    25201 aaaaggtgag caatagcatc ggatatgcaa gcagcttttt caaatcattg cgaaatcctt
    25261 caacaagccg aaactgtttg gcaatttcca acaaagttgt cggcagattc ccatgacgtt
    25321 cagctaactc gatctgtacc agttgctctg ctccatatcc tatttgcgaa agtacctcac
    25381 taaacgcttg cccttccttt agctgatgtt ggatatttgc caaaataaca ggtggaaacg
    25441 aacggatcgt gagtaataaa taaagggatt cttgcagact aaaaccagat aaaagcagtt
    25501 ctcccatggt ttgtgcaaac tggttttgct ggcttcttgt taatccttta gaatagttct
    25561 gactgccaaa tattttcctg agcttcttca taactttcct cccatgattt ttttactgat
    25621 tcttcctcca taaattgata actccataaa acagaaggat ggtgattggc atcttccaaa
    25681 agttcttgat aaatgacacc agctaaacat tcagatagct cttctttttt ctttgtcagt
    25741 tcatggattc tgaccaatgt accttcaaga ttgcgtgcat gtacggttgc aaaaacccgg
    25801 tgccctgtta gtgctgctct taccgcagca tgtgcagttg attcatctct tatctcgcca
    25861 atgattagca agtctggacg atgccgtaat gcgagtctca aaagtgtatc ataagtctgt
    25921 gctattttcg gattgatctg tagctgcaga aaaaacggtt cttctatctc tacaggatct
    25981 tcgatagtaa tcacttgcaa tttttcttcc aaagccaacc gatacattag agaagtcttg
    26041 cctgatccga ctggtccgct gaaaaggtat aatccacgtc tgacggtttt ctgtaaaata
    26101 gttgcaaaat ctcgagaact aaagcaacga tggggtgttt caaaagtacc gtataatagc
    26161 cgaatcacta aactctctcg ttgaaggtaa tctcctacag aagacaaccg cagacgaact
    26221 tcctgttttt ctaaagaata agtaaatgca cctaactgag cttttctttt ttctccgata
    26281 tccatctgtc ccaaatactt aaatcttgag atcagttgct gtccatctgc cgaagagact
    26341 ttcttccaga atgttcgttt ttttccatag cgaaaataga tgtaaaatac ttctgaagct
    26401 ggcaatatat atacgtcttg ggcatgtttg gcaaaacttt cacttatcat ttgatccgat
    26461 aatcgtttta tttccattat tctcccctcc acaaaaaaca tacgtctttt tgatcttttt
    26521 gtttcaaata aaatgaattt acaaaaaaat attggcaaaa accgaattta tgttatactt
    26581 ataaaaattt atcttgggag gcactccatt ggaaacaaca ttaaggaaat atatcgaagt
    26641 agcaagtaaa aaaaagcctg ctgacctagt gatcaagcat gctaagatcg tcaacgtctt
    26701 tacgaaagaa atcatggaaa aagatgtagc aatctgcgaa ggaatgatcg taggaatcgg
    26761 tgaatacgaa ggaaaaagcg tacataatgc taatgggcaa tatctagtgc caggattcat
    26821 cgatggccat gtgcatatcg aaagctcact tttatcacca agcgaatttt caaaagtatc
    26881 cttgcttcat ggagttacta ctgtagtgac agatccccat gaaataggaa acattgctgg
    26941 gagtacaggt cttgagttta tgatagaaga tgcaagacaa acactcatga atatcttttt
    27001 tatgctacct tcttgtgtac ctgtaacccc ttttgagacc aatggtgcaa atttaaatgc
    27061 aaaaaaatta gccccttttt tacaaaaacc cgaagtttta ggcttagctg aagtcatgaa
    27121 ctatcaagca gtagcaaata atgaaacaga catcctagaa aagatccaat tgatgcacca
    27181 gcataagaaa aaaattgacg ggcatgcagc tggtatcggc atggaagagt taaatgttta
    27241 tcctgcagca ggtattcgga cggatcatga agctacaact gcaaaagaag ctaaagaacg
    27301 tttggatctt ggtatgtatt taatggttcg agaagggacc gttgcaaaag atctggcatc
    27361 gctactaccg gcgatcacac ctgaaaattc aagaagatgc ttctttgtca ccgatgataa
    27421 actgatcaat gatttagtaa aagaaggatc aatcgatcat atcgtccgtc aagctattgc
    27481 acttggaatc gatccattac aagccattca gatggcaaca ttgaatgcag cagaatgttt
    27541 tgatttgaaa acactaggtg cgattgcccc tgggtatcaa gctgatttct tcttaacaga
    27601 cgatcttacc aagctgccaa ttcatgaagt tttctataag ggaacattag ttgttcaatc
    27661 tgggcaactc caagaccatt tatttgctga tcattcaaat tcatatacgt gttgtctgcc
    27721 aaaactaaac gctcaaccat taaatccaca ttcgttagag ctgcttcttt cttcttcaca
    27781 agcccatgtg atcgaaatta tcccgaatag tctagtgact aaagatttgg tagaaaaagt
    27841 ggaccgacaa ggcgaacgat tcattccttc aatcgaaaaa gaccaattaa agattgcggt
    27901 gatcgagcgt caccatcaaa cgggaaacat tggattaggc atagtgaaag gatttcagct
    27961 gaaacaagga gcgatagcga caacgattgc ccatgactcg cataatctag ttgtcgttgg
    28021 gacaaacgac gaggatatgc tgtatgctgc taatcttttg attcaaaaag gtggcgggat
    28081 gaccgttgtc aacaaacagc aggaaatagc ttgtgtttct cttcctatag gcggaatcat
    28141 gacacaggaa cctttttcga tcgtctatga acagttgaat gaattaataa ctaaagcaca
    28201 tcaactagga gcagctaaaa cctttgatcc ttttttgaca ctttctttct taacattatc
    28261 tgtcataccc gaattgaaga tcaccgacaa aggattattt tcattttctc gcttccaatt
    28321 gattccacct tgtggaaaaa ccgatgaaat aaatacattt taaatcaaat aaatcccaga
    28381 aaatacatta catctgtaat gttcattttc tgggatttat ttatggatat taatgttttt
    28441 atgaaagcag aaagttagtt tcacagactt ttctttttgg tcttatcttg aaattgagct
    28501 tctactcgat aaatacgttg accttttgaa gaaaattttt cttcatactc cgtcataata
    28561 ttgccttcaa attgactttg atgtaaatct aaccatacct gtttgaggat catgccatat
    28621 tgagaaaaac ttgccagaga atactcaaac aatccttgat tatctgtttt gaaatggatt
    28681 tctccatttg gacgaagaat ctgttcatct acagcaagaa aagtcttata tgtcaatcgc
    28741 cgtttttcat gacgtttttt tggccatgga tcggaaaaat tcagatagat ttgatctact
    28801 tcgctgtcag cgaaatactc tgtcaacgct gatccatcca catgtagaag tttcaggtta
    28861 ggaagcggtt gttcgatcaa cttgtctagt gcaatcgaaa caacactgac ctgcatttct
    28921 actccaatat aattgatttc aggatgggct tttgccattc cagtgatgaa ctggcctttc
    28981 cccataccaa tctcaatgtg gattggatga tcgttttcaa acaattcatt ccattttccc
    29041 ttccacaatg ttggatcact aattacaaaa ttcgggtgag ccgctaacat ttccgctgct
    29101 cccggacgat ttcggacacg cattatttat ctccattcct ttcctctaat taaaaaggtc
    29161 tggccaaatc tccgcagagt ttgggccaaa cctcagccac tgaaaattcg ttttaattgt
    29221 aaaatttctt cgttcatccg tcgatgatct tcacgtttgc aatatctggt gatttcttgg
    29281 agcaaattca tggccgcata ccaatgaaat ttttcaagca tttcgtcagt cgggcgtatg
    29341 ccataactga tcagccactg attccagcta gacagtggca cataatttcc taaaatcgtt
    29401 gcaatatcca ccgcagggtc tgcaaacatg attgaatccc aatcgactag atataaataa
    29461 tttttacata ctaaccaatt acgatggttt acatctccgt gtactactga taagttttcc
    29521 ttatctattg acgggatctg ctcacgaaga aaacgatgga caatttgcag aaattgattt
    29581 ttctggagtt cttccggtaa ttctttttca tatttttgca gcatcttctc cggcgtcatg
    29641 acacgaccac cgatacgggt cagcatggtt ttaagagaat ttgaatgatg taaatggtac
    29701 aaaacatcaa tcacgtcgtt tctttgacca atctcattag gagtcagcaa gcgaccttcc
    29761 agccactctt gtgctgtcaa agtatcgcca ttccccgttc gttttgtcca aacaagtttt
    29821 ggtgtaattc cttcccttga aagagcagcc aacatcggag tcgtgtttcg tttaataaag
    29881 actttgtcct catcacgcat gcctatgtat gtttttcctg tgtcgccttt gatgggacgt
    29941 aaccgccact cttggtctaa ctgaaactcc atcatgcatt ctcccgtcct cttactcttt
    30001 gttttgcagt acttattatt ctagtcgcct gaacactaca cgtcaagcac atatacgttc
    30061 tttttcattt aattgccatt caaaaaacca gcttttctca aaaaaaatag aacgaacagc
    30121 attcttactg tcaacgctct atttttcatt ctcattttta ccctacacag ctttgttttc
    30181 agtctgacat cttcttcaaa cgagttggta catatagttt aacaaagagc atagtaacaa
    30241 tcaaataagc cgctagcgga acccatcgct gctgcccatc taatacgact gcagcaatca
    30301 aaccaaaaat aaccgacgcg ataattaata cccatgtgag tagatactga atcgcctgct
    30361 tcttctgatt cttatggatt ggataaagct gaacgagaac catatagcgg aactggttcg
    30421 ctaaaggtaa cagttggaaa ccgatcaaat ataaaaataa gaaaccgata atcagactaa
    30481 aataaaagtc gactactccg gcaatcaata acccacctat aattactaat cggaaataaa
    30541 gaccactaaa atcagaacct cttgccaatc ttcttgcaaa caagtaaaga taagtgtttt
    30601 ttgattctct tttaatatga gtcaatatag gatccaaata gcgtcttctt ttgatctttg
    30661 ccgtaatttc aggaacatct gtgaataaat tgataaattg atagatctga tgcaacctat
    30721 gttcttctac atgcaccatt ttctcccaat ccaatccttt ttcttgttgc ttccacaaaa
    30781 accagtaata tagacttgcc tgtactaatg cacctgctat tcctgcccat attagccaat
    30841 aaaatgatag aaacaaaaag atcagagaag aaaaaaacca gcctaatttc caataacgaa
    30901 taacttgttg atctagtcga aacaattctg ctctcttgat ttgcagatga ctgaatttca
    30961 aactccataa tatgatcaga tagacgaaaa acatcgaaaa ggattgctcc gtactaacca
    31021 ccactaatgg catggccaat ccgcatgcca atatcaaaaa gatcaacgga aaaatgcaag
    31081 aatagcgaaa agctcgttcc aaataggttt gcatttcttt ttctttagga agtaaaaaaa
    31141 caatatctgc tgcctctgca aaagtagcca tcttacccac gaataaacag agcatccaaa
    31201 atacagagat tatgagccct ccaattgcaa acggtggctg cagggttttc aaccaattag
    31261 aataatataa tcccacaccg ccaatcaaga aaagacaaac taaaacaaaa tgatcgttca
    31321 atacataacg catatatttc atcattttct tttgatgtct tgctaaacgt ttttgaaaaa
    31381 aactactcat gtgcctgctc ctttgttaaa gcaatataga tttcgtccaa agaagaatcc
    31441 ggcagctgaa attcttttct caacatctca agagaaccgt ctgctcgtat ctctccgtta
    31501 tgtaagacca caaaacgatc acaatatttt tctgcagtag ctaaaatatg cgtagacatc
    31561 aaaataccgg ctccttcttt tttcatttct accatcaatt caagcaatgc attgatagct
    31621 agtggatcca agcctaaaaa tggttcgtcg atgatatata aagaaggctc gatcaaaaag
    31681 gcgcataaga tcatcacttt ctgtttcatt ccctttgaaa aattagctgg gaaccagtca
    31741 agacgattag ataaccgaaa agtctccaac aacttttctg ctcttttcat tgctatatcc
    31801 ataggtatat cgtaggccat cgcagtgact tcgatatgtt ctctcaatgt caattcttca
    31861 tataaagaag gagtctctgg aatataccca atttgtttgc gatattcttc cgtatttttc
    31921 tttaaggata gatgattgat ttctatcgtt ccttttgaag cttccagcag accgataatg
    31981 tgcttgatgg tcgtactttt ccctgctccg ttcagtccaa tcaaccctac tatctctcca
    32041 ggctgtacat cgaaactgat atcttttaac acagggagat gtccgtaccc ccctgtgaca
    32101 tgatcgataa ttaaactcat tctttttcct ccgccattta tttttattta ttataccacg
    32161 aagaaaatga cggtattttg tttttttcct tacaatagct ttaacttatg gtaaagttaa
    32221 gctgaagtat ttattcttta tagaaaggat gttttatatg gaaaactgta ttttttgcaa
    32281 aatcatcaat caagaaatcc caagctataa aatatatgaa gacgacaagg tctatgcttt
    32341 tttagacatc tctcaagcaa cgaaaggaca cacattagtt gtgcctaaac gtcatgtagc
    32401 agatattttt gaatatgatc ccgaacttgc aggagaggtt ttttcgcgtg tgcctaagat
    32461 tgctcaagca ttggaaaaag cttttccaga aatgaaaggt cttaatatct tgaataacaa
    32521 ccgtgaacta gcctatcaat ctgtttttca ttcacatatc catttagtcc ctcgctacag
    32581 caaagaggat gatttttcta ttcactttgt caatcatcaa gatagttatg gatcggaaga
    32641 actaaaagct attcaagaaa caatcgtaaa gcaggtgtcc tgcgatgatt aaaaactttt
    32701 tcaaaggact gttatttgga gctgctgctg gcactgtcgg aggtctgtta ttggcgccca
    32761 ggagcgggaa tgaaacaaga aagaaactga tcgaagaatt ggaagaagca acaactctga
    32821 caaatgattt aaataacagc ctgaatcatt ttaaaagcgc attgatcgaa acaaaagaaa
    32881 cagctgaatc tgtgattcct gctttccaag aagcaatcaa aaaagatatc gaagatttta
    32941 aattccaagc cgaaccacgt atcacacaaa tcaatgaaca agtagaaaaa ctaaaagctg
    33001 atctgccaga gtatcctgaa caaatataat aatgattatt ttctcaacag atttaactat
    33061 ataagtgaga aaagtttgcg tatttcctag gaatgtgatg aaattttgaa ttttgtttga
    33121 aactaccacg acaacgtttt taaaggaaat gggatatggt atagttaata tataaaaata
    33181 ataactaatt acaggagtgc aaattttaat gaaaaaaaaa tcaattattt tggcagctac
    33241 tagtgcgttg gctgttttaa ctctagcagc ttgttcaggc gatactaata aagatatcgc
    33301 aacgatgaaa ggcggaacga tcactgtatc tgatttctat gatgaagcaa aacttgaatc
    33361 atcgaaccaa tccttagtac aacggatgat catttataaa gtattcaata acaaatatgg
    33421 cgataaagta accgataagc aagtggatgc agaatacgac aaacaagcga aatcattagg
    33481 tgatactttt gaatcacaat tagaagctgc tggatatacg aaagatactt ataaagaata
    33541 tattcgtaac aatttagctt ttgaagctgg cttgaaagct cacgtagata ttacagatga
    33601 cgatttgaaa actgcttgga aatctttcca tccagaagta gaagcacaaa tcatcaaatt
    33661 atcaagtgaa gatgaagcaa aagatgtgaa aaaatcagcg gatgatggag atgatttctc
    33721 taaattagcc aaagacaaat caactgatac agagacaaaa gaagatggcg gtaaagttaa
    33781 attcgactca acgacaacaa ctattcctgc agaagtaaaa gaagcagctt tcaaattgaa
    33841 agacggtgag atctccgatg ttattactac aacaaatcct acttcttatg cgacagaata
    33901 ctacgttgtc aaaatggtga aaaaccaaaa taaaggcaat gacatggata aatacaaaga
    33961 tcaactaaaa gatatcgcta ctgaaactaa actaagtgac aatgcattca caactaaagt
    34021 aatcggtgaa gaattaaaag atgcaaatgt aaaaatcaaa gatgatgcct ttgaaaatgt
    34081 gttgagtgca ttcacaacaa cttcatcaag tacgaaagac tcaagtgaaa ctacagcttc
    34141 aacaaagagt tctgacacaa aatcaactga ttcaacaaaa gaatcttcaa ctgaagaaac
    34201 aactgattca tcaaaataaa agaaaacaaa aaaagcgaag acttatgtct cgcttttttt
    34261 gttttgaatg aaaaaaataa atttttgcta taaatctttt ggtacataga aacttcggtt
    34321 gtccataccg aaaatccgat cggtgtattt tcctggttct gtttgacgaa tcgaagtcaa
    34381 catcatctgc atcgaagcat cgatattgtc gatttgatgt aatatttctg cttccattat
    34441 tcttggacgt acaggtgagc catattctaa taagccatgg tgtgctaaaa ccatatgacg
    34501 taatacgaga acatcttcgt cagcttcgct gattttcaac gcaagacagg ctttagttat
    34561 ttcttcgtca atcaatacca gatgaccgat caaatttcct gcaagcgtat attctgtcga
    34621 cataggaccg gaaagttcaa tcacctttcc taaatcatgc aaaatgatac cagcatacaa
    34681 caatgctcga ttcagctcag gatattcatc ggcgattgat ttccctaagt gaagcattgt
    34741 caaagtatga taagctaatc cactggcaaa tgcatgatgg tttctttttg ctgccggaaa
    34801 ctcaaaaaat tgtttttgat atttattaag tagatagcga acaatacgat tccaatgcgc
    34861 attggtgatc tcaaacaaca attgattgat ttcttcttcc atttcttctt tcttcaaagg
    34921 ggctctttcc atgtataaac taggctctcc tggctcatct acccttgtta agcgcatatg
    34981 aagaatcttt acttgagggt ttccttgata aacctctctt ttaccgttta gaaaaacgac
    35041 tgtgccggct gtaaatttat tgatttcctc ctccgaagca tcccagtatt taccatcgat
    35101 agtacctgaa gtatcttgga aagtaaaagc aataaatttt tttccgtttt tagcgattct
    35161 tacatctgca cttttgatca ggacaaattg ttcaaactgc tcatcgatta ctaaatcacg
    35221 aatttttttc attatttgct ccttctaatt gttttacgat ttggttcatc tcatggtaat
    35281 agcttaccat ttctttatca gaagaaaaac aaattatttg atacttttct ccaaatttag
    35341 ctaaaagttc agctaattga tactttcgct gatgatcata atgaagccag ccatcatcaa
    35401 tgatgatcgg gcatatcgaa cggttagctt ctaatgagag gtaagcaaat ctcatcgcca
    35461 taatcagctg atctttcgta cctgtagata actcatagat gggaaattct gtatatccgt
    35521 ctgttaaagt cagtgtatca tttttcagta atatcttctg ataatttcct tttgttaaaa
    35581 tacctaaata atcacctgct gctcttaaca actggggtaa ttgtctttca ctcatctctg
    35641 ttgaaagatc acctaagaat gtcccggcga tttcaagtgc tccccattcc tccagtaatt
    35701 cctgtaattt tgctttttgc cggctagctt gttgataaag ttcgtccaat gtgccgtctc
    35761 tttgcaactc ttctacttga agttggagtc gttgccgatt ttcacttgct tctttttctt
    35821 cttgctggag ttgggattgc ttttctttca gttggcgcag tcttacagat aatttctctt
    35881 ttgtcagctc ttcaggaaat agtggagtaa gcatttccga taattcattg tatcttttca
    35941 tctgctgttc ggcttgtttg gctttttgta aaaagacagg tacttccgaa ggataagata
    36001 agcctgcttg agtcaattgt tcgctgtatt cttgtatcca tactttttgt ttctttttca
    36061 attgattgat ttcttgcttc aataaagtat tttgctgata gcttctggca aatttcatct
    36121 gttccatctc ttgtataaaa cgggaaagca ataccatttt ttctgaaatt gtcttatctt
    36181 gtatcggtaa ccattcttta acaaactcaa atttttggtc gatttgtcgt agtttttctt
    36241 gtatttcctg ttctctttta tgtttccgct caaatacgcc atataatttc tgataatcta
    36301 tgacttcctg tccatattct ttcatgagtt tgagcgtatc catctttcct aagtgccgat
    36361 gatccgcaaa acgatatact tcttgctgaa gaatctgttt ttcttctcca gttctctttt
    36421 gtttttcttc tgcttcagct aattgagcta aggctagatc aagttgtcct agtttttctt
    36481 gccacattcc ttttatctca gagaaatcat ttttagtttt aatttttggt tgccttttgt
    36541 agaagaaata gccaatcatg cctgcaccaa ttagtaaaag gatcactttc aagggcattg
    36601 gtacaaatat acctgcaatg attattgctc cgccagccat cagcatatac ggtttagatg
    36661 aagagactgg tgctatctct gactgatttt gtaataatgc tggataggtc tcttccaagt
    36721 cattcacttc tgcttcaatc atctcttgct gttctttagc tatttggaga cgcacagaaa
    36781 tctggctttc tagatcagca atccttcttt cttcagccag aagatgcttc cattcttctt
    36841 catataaaaa ttcttccggt aacggatttt tctgccaatg ccacctttgt tccaaaatct
    36901 tcaattctga atttgtttct ctagtctcaa tttgtaatct ttgccattct tcgtttaatt
    36961 gttccgctgt aaattgaagt tgctggagat cttttagttc ctgctcctgc tctaaataaa
    37021 aataatatct ggctgactgc tgatccatcc cgctatgctt ttccagtgtt gcttctaatt
    37081 cttccaaacg ctcatttgtt tgctgatacc gtttggagaa agactccaga tctaaaagaa
    37141 gttgctcatc aggaagacct gtagtgctgt tttttttgag agattgccat tcttcatata
    37201 aagaaaaatt aagttcttgc tgtctggcta atgaaaattg tttgttcgcc tcttttaatt
    37261 gtgccaataa ctgctgctgt tgcgactcgt attcctgttc ttgttggatc aacttgccaa
    37321 aataagcttc ctgttcttgt ttttgatgaa ttttttcttg cagctctttc cattcattta
    37381 atttttgatt gattagtaat ttttggcctt ttttcttgaa taattgttgt gctttctgat
    37441 aatattcttg ccgcttttgg aatacttgag aactgcccgt gatgcctaaa gaaatcagcg
    37501 catcatgtag ctctttttct ctcaaatgat ctagttccat caactgctct tgctggaaag
    37561 taaatacctg ctggaataac tctttcgtca aaggtgcgat cagtttttct agtaactcat
    37621 cacttcccac ttgatccccc agccacacct tcgattttcc gcgattttgc tgcttgtagc
    37681 gctcgacagc aaattcacca tatacaggat gtttaagcca tattttgccg ccataggctg
    37741 agccgtcttt tggtgtataa tccttttttt tacggccttt tgaagggaaa ccaaataata
    37801 ttgcttgaat aaattgataa atcgttgatt ttcctgcttc gtttccccca tagatcagct
    37861 gattgcctga ataaaagtca aaactttttt gacgccattt tccaaaagca gcaatctcga
    37921 tccttaaaat cttcattcct gatcctcctc aaataaaaaa tcttgattaa gcagctgctt
    37981 ggctttttca agtgtttctt cttgatattc cggcagttcg ctcaatattc tagccgcttc
    38041 ttgatgacta tatacttcat ttaaaatttg ttggaaatct tgaggtgtct gatagtattg
    38101 aaataactgt tccatcaatt tagcagaagc agcaagtggg gtcttcgtat aaaaagtatt
    38161 ctctatgaga gaaatacgcc aaataaagaa atcatttatc ctatctgaga attcctctaa
    38221 caaatagtct aatagctctc ctgattgtat ccgttccaaa acatctgtat tgagatggtc
    38281 ataatctttt aattttatct caattaaact aaatccatcc tctacttttg aaagctgctg
    38341 acgaatgtga acaatgacct cttttgtcgt tcttgctttc ctcaatgata tttcctttgt
    38401 cttccaatag acctctgcta ctttaatcgg atgtatttgg caacttcctt tacttaattc
    38461 aactagtaga atagatgtag aagcttgttc ctttttagta tgaccttgag gcgcacctgg
    38521 ataaacaatt gtttgctttt catttaagac cgttggcaca tggatatgcc ctaaagccca
    38581 ataatcgtat cccttttctt gcatctttga gggttggaaa ggagcatagt ttcctttttg
    38641 agagacgcca ggttccccgt ggtacaaccc aatatggtaa tctgtgagtt ctctagaagg
    38701 aaattccatc actttgtctt tttgaatcca cggttgacga taactaaatc cactgagaga
    38761 gacagtttct ccacttttta tcgttatttt ttttgtctct accgtttcgg atgtaaataa
    38821 atggacattt ttaggaaagg agaaccaata ccgctctttt tgatagaaat catgatttcc
    38881 gaatattata aatacaggta tatggttctt ttctaaccgt ttcatctgtt ctgaaaaatg
    38941 tttctgtatt tttaatgaag gacgattttg atgaaaatta tctcctgcaa gtaaaacaaa
    39001 atcaacgtta tttattattg cctgttcaat gatatttgat aaaacggtca gattcgtctt
    39061 caatagtttt tcctgtacag ttttatcaag agttgtcagt ccttcaaatg accgatccat
    39121 atgtaaatct gccgcatgga taaatttcac cattaaaaat ttcctccttt ttttctttta
    39181 tcataccatt ttcaatgttt tttttcatca ataaccgaaa atttgttcgt attatgcaaa
    39241 gaaattacga taaatggccg acttcaaaaa ataagtttat tcgaaaaaaa gattgtgaca
    39301 tgatttttgt cacaatccta aatatctatg ttatattcgc ttcgtttgac actgaagaag
    39361 tactatttgt actggaattt atgctccatt tttccgtatt tagaattcta tgttctatac
    39421 taccttaatt agtttatatg gtaagaattc tttcaaatgc ttgagatact ccgtctttat
    39481 catttgaaac agtgacataa tcagcttttt tcatgatttc ttgatgacta tttcccattg
    39541 caaaactaat atctgcactt tcaaacatag ataagtcatt taaactatct ccgatcgcga
    39601 ttctcaaagg tttctttaaa ttgtagattg attccaaacg atcaaacacc acacctttag
    39661 atacattttg aggtacgatt tcaatattta caggtgccga ttcagtaaca gacacattta
    39721 gcatcttttt caaatggtta aatatattag ctttcaattg acgattagga ataaaaataa
    39781 aaaatttgta aatgctgata gcatcctttt ctataatttc ctcaatatta ttataaggga
    39841 ttgaacaaat agatttttca tactcaagct catagtttag ctctttgata gaaaattcac
    39901 tccctacctc gtgaaaaatt tttttcattt tatttttata attctgagat tcataacttc
    39961 cgagatttgt atatattttg gttggaaatt tcgataggac agaatcaatt ttttttacat
    40021 catttttatc gaaggaagac tcaaatatct ttttttctcc atcaaatcca acagaaccat
    40081 ttaaccctac tgctggtatc cttaaaccag attttttgag aatattatta atgtcttcta
    40141 cttctcttcc agaacaaatg aatggaaaac agtcttccgg taaatgtttc attgttcttg
    40201 catttttttg actaatgctc tggtcagaat tcaaaagagt accgtctaaa tctataccaa
    40261 ttagtttcat ttcatccatg tctcctatac gttgattata aattcactgc ttattgtaag
    40321 tctaaatttc tcacaaaaca tatactaatt atacctaatt taagtaagta caaacatcat
    40381 tttaagaata ggaaaggaca tttatatact attctctgta tttttgattt ataaataaat
    40441 atgaattgaa tccaaatcag ctatattact attttataat aaccaactat aaaaaacgaa
    40501 gttaggtgaa aaactaactt cgatattcat aaaagactct attagttcaa cttagcaaaa
    40561 gatttaaaca aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DACEJR010000016        39218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEJR010000016 DACEJR010000000
VERSION     DACEJR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.016"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(55..402)
                     /locus_tag="IW651_000890"
     CDS             complement(55..402)
                     /locus_tag="IW651_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813637.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
     gene            complement(395..697)
                     /locus_tag="IW651_000891"
     CDS             complement(395..697)
                     /locus_tag="IW651_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813638.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(913..2304)
                     /locus_tag="IW651_000892"
     CDS             complement(913..2304)
                     /locus_tag="IW651_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0813639.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(2289..2900)
                     /locus_tag="IW651_000893"
     CDS             complement(2289..2900)
                     /locus_tag="IW651_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0813640.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(2912..3100)
                     /locus_tag="IW651_000894"
     CDS             complement(2912..3100)
                     /locus_tag="IW651_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813641.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(3114..5759)
                     /locus_tag="IW651_000895"
     CDS             complement(3114..5759)
                     /locus_tag="IW651_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813642.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDVRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQEVKEKKRPRF
                     TYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(5746..5964)
                     /locus_tag="IW651_000896"
     CDS             complement(5746..5964)
                     /locus_tag="IW651_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813643.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(5966..7009)
                     /locus_tag="IW651_000897"
     CDS             complement(5966..7009)
                     /locus_tag="IW651_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0813644.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(7164..7490)
                     /locus_tag="IW651_000898"
     CDS             complement(7164..7490)
                     /locus_tag="IW651_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813645.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(7498..7701)
                     /locus_tag="IW651_000899"
     CDS             complement(7498..7701)
                     /locus_tag="IW651_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813646.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            7776..8426
                     /locus_tag="IW651_000900"
     CDS             7776..8426
                     /locus_tag="IW651_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0813647.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            8481..9647
                     /locus_tag="IW651_000901"
     CDS             8481..9647
                     /locus_tag="IW651_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0813648.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(9736..10128)
                     /gene="rpsI"
                     /locus_tag="IW651_000902"
     CDS             complement(9736..10128)
                     /gene="rpsI"
                     /locus_tag="IW651_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0813649.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10144..10587)
                     /gene="rplM"
                     /locus_tag="IW651_000903"
     CDS             complement(10144..10587)
                     /gene="rplM"
                     /locus_tag="IW651_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0813650.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11106..11573)
                     /locus_tag="IW651_000904"
     CDS             complement(11106..11573)
                     /locus_tag="IW651_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0813651.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(11693..13231)
                     /locus_tag="IW651_000905"
     CDS             complement(11693..13231)
                     /locus_tag="IW651_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0813652.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            13311..13997
                     /locus_tag="IW651_000906"
     CDS             13311..13997
                     /locus_tag="IW651_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0813653.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(14198..14725)
                     /gene="msrA"
                     /locus_tag="IW651_000907"
     CDS             complement(14198..14725)
                     /gene="msrA"
                     /locus_tag="IW651_000907"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0813654.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14809..15486)
                     /gene="pgmB"
                     /locus_tag="IW651_000908"
     CDS             complement(14809..15486)
                     /gene="pgmB"
                     /locus_tag="IW651_000908"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0813655.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(15483..16613)
                     /locus_tag="IW651_000909"
     CDS             complement(15483..16613)
                     /locus_tag="IW651_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0813656.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(16613..18796)
                     /locus_tag="IW651_000910"
     CDS             complement(16613..18796)
                     /locus_tag="IW651_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0813657.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(18793..19818)
                     /locus_tag="IW651_000911"
     CDS             complement(18793..19818)
                     /locus_tag="IW651_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0813658.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19863..20690)
                     /locus_tag="IW651_000912"
     CDS             complement(19863..20690)
                     /locus_tag="IW651_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0813659.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(20687..21754)
                     /locus_tag="IW651_000913"
     CDS             complement(20687..21754)
                     /locus_tag="IW651_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0813660.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(21780..22616)
                     /locus_tag="IW651_000914"
     CDS             complement(21780..22616)
                     /locus_tag="IW651_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0813661.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(22613..23482)
                     /locus_tag="IW651_000915"
     CDS             complement(22613..23482)
                     /locus_tag="IW651_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813662.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(23552..24835)
                     /locus_tag="IW651_000916"
     CDS             complement(23552..24835)
                     /locus_tag="IW651_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813663.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24849..26534)
                     /locus_tag="IW651_000917"
     CDS             complement(24849..26534)
                     /locus_tag="IW651_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0813664.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26809..27834
                     /locus_tag="IW651_000918"
     CDS             26809..27834
                     /locus_tag="IW651_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0813665.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(28429..32082)
                     /gene="rpoC"
                     /locus_tag="IW651_000919"
     CDS             complement(28429..32082)
                     /gene="rpoC"
                     /locus_tag="IW651_000919"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0813666.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(32161..35778)
                     /gene="rpoB"
                     /locus_tag="IW651_000920"
     CDS             complement(32161..35778)
                     /gene="rpoB"
                     /locus_tag="IW651_000920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0813667.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            36506..37474
                     /locus_tag="IW651_000921"
     CDS             36506..37474
                     /locus_tag="IW651_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813668.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            37492..38397
                     /locus_tag="IW651_000922"
     CDS             37492..38397
                     /locus_tag="IW651_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813669.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            38394..39200
                     /locus_tag="IW651_000923"
     CDS             38394..39200
                     /locus_tag="IW651_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0813670.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        11059 a         8237 c         6939 g        12983 t
ORIGIN      
        1 atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt gtcttcacca
       61 acacgatttg acgaagagcc ttttattttc aatcgaccat tgttgcggac gtatcggatt
      121 ttctgtatca tttttcattg tataggtcac ttgtaataca cgatcatgtt gcgcaaattc
      181 atttcttttt tcggtcaatt caaccttttt aaacgttatt tcgaccccat ctgcctgata
      241 agtttcaccc atttttaaat ctttagttac aatttttact ggtttttctt ctggaaaata
      301 tctggcttta attgttagat agcttccaaa aagaatcaat cctagtagca ataaaagaat
      361 tcctttaaag agccactgaa acggaaaata cactttaaac atctttcccc tcctccgttt
      421 tgcatttgtt ttttagataa tacgataact tttgatcaat ttcatccaac tttcccacca
      481 caattgattc tatttcagaa attttttgtt caattccttt gctttcagga ttacttaatt
      541 ttacatttga ttggttagct aacactggta aatttgatac catcacacga aacatattgg
      601 cttttgttcc gaaaatccct ttgttctgtt cgtaaaaact gtcaaaaatt gttttagttt
      661 cttttgaaca atacacttta atatacggat cagacatgtg tttttactcc ttatcttttt
      721 ttatttaggg agtctgtagg gagcttgctc cctcagaggt tttccccatc taacttgcca
      781 gaaagtagcc ctgcgtaagc aagggcggat tgactggcaa gtagatgggt agctggcgta
      841 tgccctcaaa accgcctctt tccaattccc tccgtatcga tgggaaactg gaaagtctac
      901 tggctaattc ctttagaaat ccatctcaaa atctcgttgt ctcctttgct gattttcaat
      961 catttgttgc gtgaactcgt aatctttttt agcacgatac attttcacat cgtcattcat
     1021 tatgcgactg atttgatagt gaatctgttt agcagaaggt gtatgagaac aatttttaaa
     1081 gccctcagct ggtagaaata attctttctg atctgtcttt ttaactaaaa ttgttttact
     1141 tttagtacta cgtaaagttc taggattcac atcagtatga agtttaatcg tgtattcttg
     1201 atcatcttct aaagatattg tgtttgcata atgcctgtct acttctcgca agaaatcatc
     1261 atatttaatg atttttttag caccctcatg atcactaaac tgcttttgtg caatggaatc
     1321 taattccaca aacggtacat ttaactcccc agttttataa tcaactccac tagctaacgc
     1381 tataatttgt tccgtatcta gatcaacaaa attattcata tgtgtttctt ctaaattctt
     1441 ttcaagtcgt tcaaaaacag attcaaatga atattttttc cctaactttc ttgcactaaa
     1501 agtacgttcg acatactgac ttttttcttt tctagattgt ttttctcttc ctaaagtaat
     1561 tagctcatct ctacgctcat agaaatttac atcttgtttg taaagaacat agccaagttt
     1621 gttatttgtt tttgaaaatt gataaacttc gatgttgtat tcttttgcta aatgatccga
     1681 aaaacgcaca aatgaattta cttcacaatt ctccaacgta gcttcaatcg cattttgtac
     1741 aaactcttga taattaattt ttccattttt cagtttactt ggatcgtttg aatagagatt
     1801 cgttctcttt tctaaatttt catagtactg attgtctaca ggttcgagtc cattttcaac
     1861 aagcacctca tcgttaattt ttcgcattcg ttctaaatct ttagggtcaa tacgcaacct
     1921 cttacaggtc tccatatcaa tattattaat aacaagatga ctatgaaaat gatctttatc
     1981 attgtgactt gcaacaataa tttgcgaatt tggaaaagct ttttctgcaa attcgacagc
     2041 tatttcgtgc attttctcaa tacttaactc tttagaaata ttcttatcaa acgaatggat
     2101 aacatgaaat gcttgtgcta tatcttcaga tttattcatc ctggttctta ctaaattcat
     2161 ttcttgcaat gcttcctcac tattatcact acaatttatt cctgagactg caacgcattt
     2221 ttcttttttt gaaggattag ctgcatattg taatagcgag gataaatttt tcttgcgtcc
     2281 tttcgatgtt atagtagctc ccaaagttgt ttcacctcat cttttaaact tgcaataatt
     2341 tgaatcgagt gaattaaatt ccggtcaact ttttgggaat actcttcttt cgtatcgggt
     2401 ctagtttgct tttctaacca atattgaaaa taatcttttt ctacttctga cagtttgaga
     2461 aaagaagatg tactccgatt ggactccaaa aaaatgtcat ctggataact aatcatcatt
     2521 ttttttgcta tgctattcgc aagttcatct ttaaaaatgt tgtaacggct aacgtcaaaa
     2581 ctaaaatgca ggctcttttc tttcttattt tcagattgtt ctctaaaata tttagcaatg
     2641 atatttaatg cttttaccgc ttgattcaaa ttgttaccaa tttgatttaa ctgtttgatt
     2701 agagctaccc tatcatcttc agccaataac ggcggttcat atttgagtcc ctttaaccgc
     2761 tcacgacaaa catctgctct agaaattcct ctttgttttg caatcaatgt cagttcaaga
     2821 taagtctttt tatcgacacg aaaatttaag gcctcactat gtttgcgatt tttttcgtac
     2881 attttttgtt tatcatccat tgattatccc ctcacatttt ttctagagag caacatttga
     2941 aattgctctc tatttcattt ttcatcatta attcttcttg aatcatcagt tcaatataat
     3001 ttgtgaccgt gattttttca tttgtcgctt tctttactga gtcgcataat tcggcaagtt
     3061 ctaagaaatc ctgcgtacta atctgtagaa ttaattgcat gacaatcacc tctttatttt
     3121 ttaaatagac tatcaaacag cggatttggt ttatcagaaa tatcctgaaa cagtggatta
     3181 ttttcataag taaatcgtgg acgtttcttc tccttcactt cttgttctac aatcactttt
     3241 tcgttaggcc tttcttttcg ttcctcgata atatttattt tttgcgtctt atctccctga
     3301 cttgcttcag ctcttgtttc acgtacttct accgttcttg gttgatttga cgcactactt
     3361 tgtacatttt ctcgtacatc tactgttcga tttccatttt gactacttgt agtattctgc
     3421 aagttttctc gtacatctac cgttctcggt tgacttgtcc cactactttg tacattttct
     3481 cgtacatcta ctgttcgatt tccattttga ctacttgtag tattctgcaa gttttctcgt
     3541 acatctaccg ttctcggttg gcttgtccca ctactttgta cattttctcg tacatatact
     3601 gttcgatttc cattttgact acttgtagta ttttgtaagt tttctcgaac atctactgag
     3661 cgcctatctt gactacttgt agaattttgt agattttctt tgactgattt ttctaatccg
     3721 gtatttgaat ttttagcttc ttggattctt tctttagtca acgcttcttt ttcgtccata
     3781 ttagcgcctg tacgcccttc tacacgtttc cttaacattt cctcacgatt cattgcttca
     3841 ggcgtgtatt ttgcttcctg tcgcttctga ttaattaata cttcagcaga cttagcttga
     3901 ccgtgcatag catttttacg ttcttcaata tcttgtttaa ctttttgctg aattgcactt
     3961 gcaggattaa tatttgattc agcattattt ttcattgctt cacgcatacg ttcagaacta
     4021 gacataattc tatcagcgtt ttcaacttta gcgccatctt ttctatccga aagacgtgtt
     4081 ggcatagaat cagcatattt tccagtttca tcttctttgt ttttagctac tataggtttg
     4141 ctatttttat taatagcgta cacggtacca tcatctaaac cttctttgaa tttattcttg
     4201 gtatcattag ccatatcacg catactctta aatggttttg ttgctctttc actggccatt
     4261 gtagctccat ccgaagctaa gcctgataat cctcgttcac gagcataccc aagtgttttc
     4321 cccgttttat tagctttttg agaaagtgat tgaccaaagt tgcgttcagg ttttcgttct
     4381 tccccttccg cttgatcgtt tccaccacta tttttcccac ctttagatcg gttcatacca
     4441 cctgttcctt tacgaaacag catagctccc atgccaaatg ctgaagcaag ctggccatag
     4501 ccttctttta aaccaatatc cacgccaaag taacgcatga ctgttccact accctttatc
     4561 agcacaataa cagcgcagac gtacgctact gcttttataa aaggatttac agaagaaccg
     4621 ctacctgttc cgagataatt aacaaacatt gcaaacatag acaatccgaa cccttgaaat
     4681 ccgactgtca aataacactg taaaatatct gacaaaactt gctttgatcg ttggcctgtt
     4741 tccaagtcag tagcaaatac taaaactcca agtacgcgtt taaacgctag ctcgagtatt
     4801 gctgtaatta taatgaaagc actaaaaaca tacgctaccg ctaaagcaat caatccaaaa
     4861 gaaatccccc atagatttgc ttgatagcga taatatcctg atttaatgcc ctcaacaaag
     4921 gtcgacagaa aattatccga aaatttggta gcaacaaatt cattatcaga attttcaact
     4981 aattcataac gcagattatc agctattggg ttctcttttt ccattttgtc gagtacatta
     5041 tctgtcaata cttgcgtaag atccgttttc caaaaatttt cttttgttag attgttccgg
     5101 tcattcgttt tatcgatcat ggaatattga tctgttttat tgatataagc taaatcagta
     5161 accccttgtt caatcagcga ccacgccacg ccttcgtccg aatttgtaat cgtagtcgta
     5221 tcattataaa atgttttaga aaactctatt ccgctgtgaa tcaacgttgg cattaaaaga
     5281 atcaatgaaa ttgacattac aacattcatt ccaacagatt ttagatcaac cgttccctta
     5341 cctgtaatca ttttataacc aataatcact agtgaaagta tcattaagcc aacaacaatg
     5401 gtacttaaaa cagcttgata gactttgttc aatccggtac tttctacaaa gctaaacaaa
     5461 tctaaaactt cgggaatcat attttgaatg ccatcgacaa cagcgaacat tgattttata
     5521 acagaccagc ctatccacat accgaactgg ctgtaaaaat ttccgttgta ttttttcaaa
     5581 taattctggt aaagcggatt agttaaaaac tcagtttgac tctcagtatc atcaaaccaa
     5641 ttgaaaatac tgccttctgc aatgttactc gtaaaaagaa tcacagctat tagaacaaga
     5701 aaaataaccg gtaaccaatt tgtttttcta ttagcaatct tttttttaat tattttcatt
     5761 gttacctccc tcagcccatt ttcttaggct ctcagagagt ttctgagcta tttttctgtc
     5821 aattatgata tgagttacgt aatcatcatt aacagctttt aaatcgagaa caatagtatt
     5881 tgagcagata ctctcggtta actcaagagt atctgcgttt tgatcaagac agtgaatctt
     5941 atctactaga tgattttcaa tcattttaaa ttccctcttt tttttgtagc ttgattagtt
     6001 ctaacgcttt gttttttaga gaagtaaaga tataattatt tttttgtttg atacgatgat
     6061 ctgtttttgc tcttcgtatc aagtcacgta aagtcattgc tagctgttct ctaacatgat
     6121 ctacaaaata actattatta caaatttcat agataatttt cagttgaact ttttgttctt
     6181 tttcagcaga tttaattgca taattgaatt ttttgtacca gttgtcgaat tgacgataat
     6241 cactattgct agccagtttt aagcaattca caatactttc tccccacatt tgtttaagaa
     6301 gattttcttc aataaatgtt tcgatcaatt cttcatctaa tttattattt ttagttgaaa
     6361 gtgattcaaa aagatcatca ttatgtgatt ctttaatatc tttactatct ttataagact
     6421 tatataattc tagttcattt tttgaactat cagcaaaatt gtttttgtct cttgttttca
     6481 ttgatgtgtc gcatagttca aattttgaac tatcaacaga atattgattt gttccatagt
     6541 tcaaattttg aactatcaag tttttttgac tattttcttg atgggttatc ttattaattt
     6601 tataaacatc atctttgcac agtacaggct ttaacaaata ataaaaattt gggaacattt
     6661 gcattttatt tttattgaag ccattttttc ttttcacaag aagtttaact ttttgaagtt
     6721 catctagtgc tgataagatt gtattttttg acattcccga aatttctacg agatttttga
     6781 tttcaaatat caaaaaaatt ttttgattct catcatacaa gttatttttc aatgaaagtc
     6841 tatttctatc ttttagtaac atataaacta acttagaact tagtttcatt ttcttatatt
     6901 tttcatttac gtttaaaaaa ataggatatt gatagtataa ttctgcatat atatcagaat
     6961 tagaatagcc ttgcttttca actaagcttt caacttcttt ttcattcatt gtcacacctg
     7021 cttctgattt tatttgctat aatagattca aataaagttt tctcagaact ttattattga
     7081 gtgttccccc gatctgcaaa ctgacgggaa cactcttttt aattttttct acttcatcat
     7141 tattcaaaaa aacacctcgt tttttaaatt tgatgactca atatccattt tttgcattct
     7201 tcttttgaaa acaactccat tttttctaat aaaataactg gcaatcccat tcttttaaat
     7261 ttcaaaaaag ttgtatatga tacattcaaa tatttagcca tctcttttcc ttttagaaat
     7321 ggtctttcta acccggtatc aattcttgct tgatcaatag cctcttttgt catttcaaaa
     7381 acataatttt tcaattgatt cgtttgttgt ttatctaaca ttactgtaat tcctgcatcc
     7441 attatttttc tcctctcatt ttctatttct tcaatcagcc aactcgccaa gttgtattta
     7501 tttagatttc aaatcattaa gtaaccaatt taccaacttt tgataagtag atgctctaat
     7561 actttctttg ctcccatttt cgattttgac atatgttttc cgatttatac ctatcaattt
     7621 tgctgcatcg tttttagtaa gtataagatc ggctctatgt cttcttaagc ttctaatgaa
     7681 ttgatcatta agagttatca ttcagcgcct ccttttaaat tattcacttt gtggttttta
     7741 tcttaataag cacattttaa accacgaaac gagtaatgtc aattaaaaca ttactcaatt
     7801 tgtggttttt atgctataat agtttggaat ttactatagg gggtgttact ttgaatagaa
     7861 taaaatttta tattgattcc agtaaaaaga ccattaaaca aattagtaca gaaactggta
     7921 ttaactatac aactcttacg aactattacc gtggtgagcg aaatcctaga aatactgaaa
     7981 catggaatat ccttgctgat tatttccatg ttcctgtaag ttatttgatg ggattagacg
     8041 aaacaaattc catagaacaa cctaatcttg atagaataaa aactgctcgt ttagaaaaaa
     8101 aggacaaaat agtcaagaat attgccagtc ttgactcaga ttattttact gaattatcta
     8161 atctatactt ttccatgact tctttacttg aagtcaaact tcaagaagat gactccgact
     8221 ttcttttttt aaaaagtatt ctagaggact cctttgaata cacgattcta tttgccaaac
     8281 aaaagaagtt tattaataaa gctttaaact taaatatcga tgaaagaaaa gagttagaat
     8341 ctgagttatt tggcaactta acccaagtac aggttaacaa tctaaaaaga gagataacag
     8401 ctcgattaat atcaaaactt caataaaact aattattcaa tcagccaact cgccaagtta
     8461 tcagattgga gagattagtt atgtcaatac aaaagtacac aacaaaaagc ggtgcacgat
     8521 atagtgtccg cttgtatgtt cgtaaagatg aaaatggaaa gcaagaatac tatgtaaaaa
     8581 aagggtttaa aactgaaaaa gaagccaagt tacacgaagc aagaaaaaag gtcgagattg
     8641 aagataccgg ctatcataag ccttctaatg acttgtttat aacagtgtat gaagaatggt
     8701 tgccttctta taggaatcgt gtacaagaaa cgacatatca acgtacaaaa gaccttttcc
     8761 gcctgcatat actaccaaaa ttcgggaata agtccatttc taagattact cctgttcact
     8821 gtcagaatgc agtcaatgaa tgggctttaa aattcaagaa tatcaaacag cttaagtctt
     8881 acagctctca aatatttgag tatgccattt ttgcagagct attacaccga aatcctatgg
     8941 caaatacaaa tctaccaaag cgggaaaaga aagaaaatga aaattacttt tcattagaag
     9001 aactaaaagc tttttgggat attctcaata aagaagagcc tttaaaacat attttaatct
     9061 tccagctttt gattactact ggtatacgta aaggagagct agctgctctt cattggtctg
     9121 acatttattt tgaagaacag ctaatgtatg ttagaaagag ctatgcaact attcgaaatg
     9181 aagagaagaa tgcaagaaga aaaactgtca ggattcaaaa aaatacaaaa aatatatctt
     9241 ccgaacgaat attaccaatt gatgcacaaa ctattgaaat gttaaaaatc tggaaaaaag
     9301 aacaagctcg agagcttttg cagtttggca taaatacaaa tacaaaaaat caacttatct
     9361 ttacctatgt aaatgcagac ggtgacatta atcaaccttt gcatgcggac tattcaaata
     9421 atatattgaa acgtttagag aagaaatata aatttaagca cgttactatt catggattcc
     9481 ggcacactca cgctactctt ctactagaag gtggagcaag tatcaaagaa acacaagatc
     9541 gattgggtca taaaaatgct gaaactaccc ttaacactta ttctcatgtt actgaaaaag
     9601 ctcaacgaaa tgcgttagaa aaattcattg cttacactgg tttataattt tgtcattacc
     9661 tttctcatta ccagaacgaa aaaaagacac cttctcgcaa tgagaaagtg tctgaaatcg
     9721 ttggtataac aacgattaac gttttgaaaa ctgtgaagct ttacgtgctt tcttaagacc
     9781 tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc
     9841 taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc
     9901 tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt
     9961 ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc
    10021 ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc
    10081 aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc
    10141 ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc
    10201 tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag
    10261 catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc
    10321 agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc
    10381 ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat cgccagtatc
    10441 caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga
    10501 aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc
    10561 tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg
    10621 acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac
    10681 ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt
    10741 acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga
    10801 ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt ctcctgccaa
    10861 ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt aaaattgttt
    10921 cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg ttcaatcagc
    10981 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg acgtttgttt
    11041 ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa gttcaacaac
    11101 agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat ccagatacgt
    11161 ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc gccttcttct
    11221 ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc tgcaacaagt
    11281 gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg gatttttgtg
    11341 ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg
    11401 gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat tgggtgcaat
    11461 gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac agacatttgg
    11521 cttagatctg caactaattg atttaatact tctttggttt gttggaattt catctttctt
    11581 gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa agtacaacaa
    11641 aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc aatcattttt
    11701 cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt gctaaatgct
    11761 tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca gtttcttgta
    11821 ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt tggatctttc
    11881 cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg acagtaggga
    11941 aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga gattgagcaa
    12001 acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct tttaaaatcc
    12061 gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt tctgtatgtt
    12121 gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac
    12181 caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa gcgatagccg
    12241 aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg acttcctgta
    12301 aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct cctcccacta
    12361 cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg aactgaggct
    12421 gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg atcgtatcac
    12481 caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata
    12541 tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt acttgatagg
    12601 tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca agcacttctt
    12661 ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc
    12721 tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca aaataagcag
    12781 tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc aatggtgtca
    12841 ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg gtatatttca
    12901 gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga gataacggtg
    12961 ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag acaaattgaa
    13021 tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag acaccttgaa
    13081 agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa
    13141 aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg
    13201 ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta tgtatgtaca
    13261 aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc gtgttacttt
    13321 attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag cggattccgt
    13381 taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt tcgctttctt
    13441 ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc cgctattgca
    13501 ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt ctttttgcct
    13561 ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg ctttcggctt
    13621 tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg ctattctgtt
    13681 cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg caaagtgtgc
    13741 tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg
    13801 gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag ttgcttcaga
    13861 tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt atctgtcata
    13921 aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg ctgattgtcc
    13981 ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct ctgatatata
    14041 gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca acctctccac
    14101 taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt ttacaacctc
    14161 tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg ccccaatgtc
    14221 gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg agcttgtttt
    14281 tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt ggttcgattt
    14341 ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg
    14401 caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa tcaccgcgat
    14461 cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt tccaccaact
    14521 gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta tgccctgttg
    14581 tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta taaccggatg
    14641 tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa aagcatccgc
    14701 ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca taccttgatt
    14761 tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt atatacgtga
    14821 tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta aatcaggaat
    14881 cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat gcatgttacc
    14941 agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg catttggcgg
    15001 tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg cttttgatac
    15061 ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag
    15121 gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt tcaagtactc
    15181 aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag
    15241 atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga atttctgttc
    15301 catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc ctttcagttg
    15361 ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca gccacgcttc
    15421 gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg ctgctttgat
    15481 tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat gtttttagct
    15541 gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac agctgcagga
    15601 aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc tttaccaaca
    15661 cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat cccaactggc
    15721 gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac gaggtctgct
    15781 tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc agagactatt
    15841 ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact actgcccgat
    15901 gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg gtcagccact
    15961 gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc gccttctttt
    16021 ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat cagctcaata
    16081 ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag tgcttctact
    16141 ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc gatgccgcca
    16201 tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat
    16261 tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg ttggtttagt
    16321 gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa
    16381 gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt ttgccgcttc
    16441 aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc tataggtagc
    16501 agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc
    16561 tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg cattattttc
    16621 ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg acaaaaatag
    16681 gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa acaaatagca
    16741 acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg
    16801 gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat ataactgcta
    16861 gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc
    16921 ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt tcttggatac
    16981 ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt gttttgtttt
    17041 catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt aataagtata
    17101 acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg ctacgggcat
    17161 aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa tcaataactg
    17221 gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc actggcaaat
    17281 ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca gcttgatatt
    17341 taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta ttgtcgatat
    17401 attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca tcgactttta
    17461 cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc tttgtttcta
    17521 aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca aggtctgcac
    17581 tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt ttgatattca
    17641 atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg
    17701 caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga tcataacggt
    17761 acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc aggataaaaa
    17821 tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc ttcgccccta
    17881 cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca gcgaagtcca
    17941 tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat
    18001 ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt ttctgggctt
    18061 gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc acgatgatct
    18121 tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg gtgatccggc
    18181 gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca atatcaattt
    18241 tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact cgcagttctt
    18301 tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc gttttcacat
    18361 ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc tgatggagat
    18421 tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga gcagtttcaa
    18481 tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg tatgcaagaa
    18541 ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat ctgatgacat
    18601 ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat acaccagctg
    18661 caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg ataccaaaaa
    18721 atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa tcaaactgtt
    18781 tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat ataaattttc
    18841 caccagattc tggatgacat atccttgcca gccatctgca atcatctgag tctggtcacc
    18901 gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa ctggcgtggt
    18961 cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg gatacagatt
    19021 agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga tattcaaagc
    19081 aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc caaaaattgc
    19141 atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat tttttttcgt
    19201 accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc cagtaatata
    19261 catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc cttgaagttc
    19321 tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat tcgcttctac
    19381 gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt cgtcagaaaa
    19441 acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct ctgccatctt
    19501 tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag cattagcttc
    19561 gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac tcacgatatc
    19621 gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg
    19681 tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca attctaagac
    19741 atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc cagcgcctat
    19801 gattgctact ttcttcattt atttctccct cctttgctta tttacttatt gttattcttt
    19861 ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt tttcaagtat
    19921 tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca ttcatatacc
    19981 atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg
    20041 ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt
    20101 tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg
    20161 atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa
    20221 ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct
    20281 tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg
    20341 cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata
    20401 acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca
    20461 ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct
    20521 gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt
    20581 aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt
    20641 tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc
    20701 ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt
    20761 aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt
    20821 aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt
    20881 aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg
    20941 cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga
    21001 tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg ccactcgttt
    21061 caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg
    21121 ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct ctacgctgcg
    21181 ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa
    21241 tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt
    21301 cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat
    21361 ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac
    21421 gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg
    21481 tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc
    21541 gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga
    21601 taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa
    21661 ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg
    21721 ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt
    21781 tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac
    21841 ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag
    21901 tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac
    21961 aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca
    22021 gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta
    22081 agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt
    22141 agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg
    22201 gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg
    22261 gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt
    22321 tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag
    22381 gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata
    22441 tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct
    22501 tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct
    22561 aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca
    22621 agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg
    22681 atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc
    22741 ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga
    22801 tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct
    22861 tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga
    22921 ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa
    22981 acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga
    23041 aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat
    23101 atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac
    23161 gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag
    23221 ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa
    23281 attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt
    23341 taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca
    23401 ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag
    23461 aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat agctatctac
    23521 ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta
    23581 aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac
    23641 ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt
    23701 gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt
    23761 cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag
    23821 gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt
    23881 cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc
    23941 catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg
    24001 cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca
    24061 tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt
    24121 tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat
    24181 catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg
    24241 ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt
    24301 gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt
    24361 tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt
    24421 ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt
    24481 cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc atcactttag
    24541 caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga
    24601 tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt
    24661 ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag
    24721 agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag
    24781 cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt
    24841 tccacctctt attttattat tttcagccac atatattcat acggctggaa tatcaacttt
    24901 cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt
    24961 aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc
    25021 gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc
    25081 ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact
    25141 gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc
    25201 cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc
    25261 agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca
    25321 gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga
    25381 tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact
    25441 cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata
    25501 cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt
    25561 tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa
    25621 cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc
    25681 tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat
    25741 accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt
    25801 tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag
    25861 cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa
    25921 acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt
    25981 ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc
    26041 acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc
    26101 aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg
    26161 aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg
    26221 ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc
    26281 aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta
    26341 cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct
    26401 tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt
    26461 tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa
    26521 agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat
    26581 attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa
    26641 tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc
    26701 atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt
    26761 actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact
    26821 atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat
    26881 aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg
    26941 aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta
    27001 gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt
    27061 caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa
    27121 acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga
    27181 attattattt taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat
    27241 attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac
    27301 acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt
    27361 catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt
    27421 taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc
    27481 ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga
    27541 ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa
    27601 caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg
    27661 tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc
    27721 aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc
    27781 attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga
    27841 attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact
    27901 catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta
    27961 gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa
    28021 ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc
    28081 ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag aataacagac
    28141 atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa
    28201 aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact
    28261 tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg
    28321 atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca
    28381 aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg
    28441 attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt
    28501 ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc
    28561 agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt
    28621 accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa
    28681 gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc
    28741 aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa
    28801 taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat
    28861 ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac
    28921 acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa
    28981 ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc
    29041 tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt
    29101 gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt
    29161 tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg
    29221 aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt
    29281 caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac
    29341 ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt
    29401 tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg
    29461 ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg
    29521 atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat
    29581 ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc
    29641 acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc
    29701 agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc
    29761 caagacggat agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt
    29821 aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg
    29881 agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt
    29941 ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc
    30001 atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt
    30061 ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc
    30121 ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc
    30181 tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa
    30241 cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa
    30301 gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa
    30361 ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc
    30421 tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga
    30481 atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac
    30541 cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat
    30601 atcttgagaa ggagtaacaa ctgatttacc atctttaggg ttcaagatgt tttgagcagc
    30661 taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat
    30721 ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc
    30781 gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc
    30841 ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc
    30901 ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac
    30961 aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca
    31021 ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg
    31081 tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga
    31141 tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa
    31201 tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt
    31261 caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt
    31321 caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg
    31381 aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc
    31441 ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa
    31501 ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat
    31561 cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata
    31621 ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac
    31681 atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc
    31741 catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat
    31801 atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca
    31861 aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt
    31921 aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt
    31981 aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc
    32041 caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt
    32101 catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga
    32161 ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc
    32221 cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt
    32281 caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc
    32341 tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa
    32401 tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc
    32461 gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt
    32521 ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc
    32581 aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg
    32641 tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga
    32701 cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct
    32761 ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac
    32821 tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac
    32881 ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt
    32941 tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt
    33001 tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac
    33061 acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt
    33121 cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc
    33181 acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac
    33241 cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg
    33301 gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg
    33361 tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg
    33421 gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata
    33481 gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc
    33541 ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg
    33601 aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc
    33661 gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc
    33721 aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc
    33781 cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat
    33841 gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt
    33901 cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat caatagatac
    33961 ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc
    34021 attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta
    34081 gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat
    34141 aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg
    34201 gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac
    34261 ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt
    34321 gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact gagaagaacc
    34381 aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa
    34441 tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc
    34501 gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg
    34561 gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc
    34621 aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac
    34681 gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg
    34741 atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat
    34801 gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat
    34861 ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt
    34921 caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg
    34981 atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg
    35041 acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt
    35101 atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat
    35161 cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt
    35221 acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc
    35281 acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg
    35341 gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat
    35401 gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt
    35461 gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt
    35521 tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac
    35581 aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc
    35641 tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg
    35701 taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt
    35761 tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga
    35821 tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg
    35881 cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt
    35941 ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt
    36001 catcagatag tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc
    36061 ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa
    36121 ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat
    36181 tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata
    36241 acttttactt tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa
    36301 cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac
    36361 aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt
    36421 tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt
    36481 tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc
    36541 cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg
    36601 acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa
    36661 aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga
    36721 ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa
    36781 agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga
    36841 aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt
    36901 cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga
    36961 aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta
    37021 taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa
    37081 agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac
    37141 gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag
    37201 tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc
    37261 agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg
    37321 caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc
    37381 tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg
    37441 catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat
    37501 ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt
    37561 ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt
    37621 cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct
    37681 tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca
    37741 aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc
    37801 acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat
    37861 tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt
    37921 gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt
    37981 cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg
    38041 aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc cttgcttgcg
    38101 caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct
    38161 tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact
    38221 gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt
    38281 gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg
    38341 cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg
    38401 acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc
    38461 atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg
    38521 gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc
    38581 agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga
    38641 aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag
    38701 aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca
    38761 aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa
    38821 atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat
    38881 tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg
    38941 accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc
    39001 tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga
    39061 tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca
    39121 cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc
    39181 aagtactttt atcttcataa tcagaaagag gatgggac
//
LOCUS       DACEJR010000017        39074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEJR010000017 DACEJR010000000
VERSION     DACEJR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.017"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW651_000924"
     CDS             6..305
                     /locus_tag="IW651_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0813671.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW651_000925"
     CDS             378..1739
                     /locus_tag="IW651_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0813672.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNPLKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW651_000926"
     CDS             1761..2021
                     /locus_tag="IW651_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813673.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW651_000927"
     CDS             2035..2787
                     /locus_tag="IW651_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0813674.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW651_000928"
     CDS             complement(2881..3486)
                     /locus_tag="IW651_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0813675.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW651_000929"
     CDS             complement(3539..4513)
                     /locus_tag="IW651_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0813676.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW651_000930"
     CDS             complement(4891..5670)
                     /locus_tag="IW651_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0813677.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW651_000931"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW651_000931"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0813678.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW651_000932"
     CDS             7161..8558
                     /locus_tag="IW651_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0813679.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW651_000933"
     CDS             8639..9079
                     /locus_tag="IW651_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0813680.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW651_000934"
     CDS             9227..9553
                     /locus_tag="IW651_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0813681.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW651_000935"
     CDS             9554..10303
                     /locus_tag="IW651_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813682.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW651_000936"
     CDS             complement(10383..10838)
                     /locus_tag="IW651_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0813683.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW651_000937"
     CDS             complement(11265..11540)
                     /locus_tag="IW651_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813684.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IW651_000938"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IW651_000938"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0813685.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IW651_000939"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IW651_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0813686.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IW651_000940"
     CDS             14770..17436
                     /locus_tag="IW651_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0813687.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IW651_000941"
     CDS             17800..18552
                     /locus_tag="IW651_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0813688.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IW651_000942"
     CDS             complement(18574..19728)
                     /locus_tag="IW651_000942"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0813689.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="IW651_000943"
     CDS             19913..22354
                     /locus_tag="IW651_000943"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0813690.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="IW651_000944"
     CDS             complement(22684..23388)
                     /locus_tag="IW651_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813691.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="IW651_000945"
     CDS             complement(23575..23820)
                     /locus_tag="IW651_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813692.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="IW651_000946"
     CDS             complement(23837..24688)
                     /locus_tag="IW651_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0813693.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="IW651_000947"
     CDS             24854..25858
                     /locus_tag="IW651_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0813694.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="IW651_000948"
     CDS             complement(26099..26455)
                     /locus_tag="IW651_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0813695.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="IW651_000949"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="IW651_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0813696.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="IW651_000950"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="IW651_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0813697.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31534)
                     /locus_tag="IW651_000951"
     CDS             complement(30815..31534)
                     /locus_tag="IW651_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0813698.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            31854..32375
                     /locus_tag="IW651_000952"
     CDS             31854..32375
                     /locus_tag="IW651_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0813699.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="IW651_000953"
     CDS             32434..33294
                     /locus_tag="IW651_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0813700.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33401..35794
                     /locus_tag="IW651_000954"
     CDS             33401..35794
                     /locus_tag="IW651_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0813701.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="IW651_000955"
     CDS             35976..37817
                     /locus_tag="IW651_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0813702.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="IW651_000956"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="IW651_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0813703.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12571 a         6877 c         8094 g        11532 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatccattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatccg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttatgaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaagcttgag actacgcagt ctcaagcttt tttttctata ggaaggaaat
    39061 tcattcatta tttt
//
LOCUS       DACEJR010000018        38792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEJR010000018 DACEJR010000000
VERSION     DACEJR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.018"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(66..1649)
                     /locus_tag="IW651_000957"
     CDS             complement(66..1649)
                     /locus_tag="IW651_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813704.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPMLGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEISKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSNETIQNLQISINDFKPKKQVLTEIEYYYRLRSLAVLANN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEALPWIYNKLNQEQFNKLLYNLNGNNQFYKNGNFPPFISNLLDFYNQKF
                     QVDLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1771..2355
                     /locus_tag="IW651_000958"
     CDS             1771..2355
                     /locus_tag="IW651_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813705.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFICRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            2366..2611
                     /locus_tag="IW651_000959"
     CDS             2366..2611
                     /locus_tag="IW651_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813706.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            2617..3339
                     /locus_tag="IW651_000960"
     CDS             2617..3339
                     /locus_tag="IW651_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813707.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSDNNNKDTKSSNTVVTKN
                     STTSASTTMSSMIENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWVQNIHGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3470..4213
                     /locus_tag="IW651_000961"
     CDS             3470..4213
                     /locus_tag="IW651_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813708.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKNEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWNSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRSKLIKELVQNTWDQAIVELEKENGKILANKYLIVNEIEELIGLF
                     SCKKGEVFLKSLPTYSILDSPYQLIQARR"
     gene            4219..4584
                     /locus_tag="IW651_000962"
     CDS             4219..4584
                     /locus_tag="IW651_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813709.1"
                     /translation="MSSLKDDYIQAKQEYEKLNMLKSEKVELFLTQKLAEEHISEIDA
                     KTLERYDQQFNQIHHNIILLVDQAKRRLEKAEYDLLDQGIVCLDKGIRRYYSAELKKQ
                     NVDRNALKNEILELIMQRI"
     gene            4600..5130
                     /locus_tag="IW651_000963"
     CDS             4600..5130
                     /locus_tag="IW651_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813710.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMMYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NEKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQRRYNKVFPNA"
     gene            5259..5519
                     /locus_tag="IW651_000964"
     CDS             5259..5519
                     /locus_tag="IW651_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813711.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATDDVLSSAT
                     EWFRKNEESAVHFEGIDGNTHSLFYTKDKEKAKKIIDILKER"
     gene            5756..6217
                     /locus_tag="IW651_000965"
     CDS             5756..6217
                     /locus_tag="IW651_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813712.1"
                     /translation="MSKFEFLDKSIGSAFLIDPDLFGSWAQSSGYNWAFSDDMVELLS
                     QTMNWEAYKIKRFLDVVAQPIYISVDDHWSGRELTTLKELAEYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            6335..6652
                     /locus_tag="IW651_000966"
     CDS             6335..6652
                     /locus_tag="IW651_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813713.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKIYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            6655..6887
                     /locus_tag="IW651_000967"
                     /pseudo
     CDS             6655..6887
                     /locus_tag="IW651_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7003..7482)
                     /locus_tag="IW651_000968"
     CDS             complement(7003..7482)
                     /locus_tag="IW651_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813714.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAIKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKLLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7602..8939
                     /locus_tag="IW651_000969"
     CDS             7602..8939
                     /locus_tag="IW651_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0813715.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8962..9489
                     /locus_tag="IW651_000970"
     CDS             8962..9489
                     /locus_tag="IW651_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813716.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            9476..10039
                     /locus_tag="IW651_000971"
     CDS             9476..10039
                     /locus_tag="IW651_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813717.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            10461..11198
                     /locus_tag="IW651_000972"
     CDS             10461..11198
                     /locus_tag="IW651_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813718.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDESPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11215..11631)
                     /locus_tag="IW651_000973"
     CDS             complement(11215..11631)
                     /locus_tag="IW651_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813719.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIIV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDEMYSFVK"
     gene            12023..13270
                     /locus_tag="IW651_000974"
     CDS             12023..13270
                     /locus_tag="IW651_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813720.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGIANGGNVVDISGSSVQ
                     GLKDEIANGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINSGSFETAYNVSQLAVSVR"
     gene            13539..15929
                     /locus_tag="IW651_000975"
     CDS             13539..15929
                     /locus_tag="IW651_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ0813721.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKIKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTAKNTLKKGVVKVHKVDQDGKPL
                     ENAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNTLKKGVVKVHKVDQDGKPLANAEFTLTDEKGEKEVKTTDEAGLATF
                     NIVANRTYSLEETKVPTGYTGSFKQEDITIKDDGQVFEYTATNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDQGEKVVQKTDKDGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVVDDGQTFEYTVINHRVPETVRTGGIGTSAFLLVFLVAGLGMGLLYYREQRKVK"
     gene            15978..17813
                     /locus_tag="IW651_000976"
     CDS             15978..17813
                     /locus_tag="IW651_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0813722.1"
                     /translation="MKSKKVFSNLMLFGTLLGVGLGASSISSSFGLGEQVAYAQQNYN
                     YQGTGTIHIHKVANTNGDGKPVSDIDNKGKEIALPNGVQPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPVQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDIGEDVQYTLKPSIPGNIG
                     DYEKYVVKDVLGEELTYDSSEVIEGMTLNTDYSIERDGQTTNYVFTDSGMDKLQKKFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNRVTYLKQDPTTKAITWVEDKSKATEFVSGTDGKFQ
                     VEGLQYTYDVDFDNQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSK
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17882..18745
                     /locus_tag="IW651_000977"
     CDS             17882..18745
                     /locus_tag="IW651_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0813723.1"
                     /translation="MSMKKKNIKLIILRIGMLLCLGVGLYPVITQAYTYYQTQQTLNA
                     YKEKYDEASEKENEEIFKKYSEINRKRKETEQTLVTIPEDNNNIPSNGNLQKEKLGEL
                     VGEILIPKINVDLPIYNGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFINVAGKKLAYKVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVG
                     ENSHRLLVRGVRIPYKKNIVEKEKKKEDLKVKAQINWYPIIVVGVLSIIALIVIIKKI
                     VAKRRGMKNEE"
     gene            18735..19814
                     /locus_tag="IW651_000978"
     CDS             18735..19814
                     /locus_tag="IW651_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0813724.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNNQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSEAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            20153..20953
                     /locus_tag="IW651_000979"
     CDS             20153..20953
                     /locus_tag="IW651_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813725.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            21041..21847
                     /locus_tag="IW651_000980"
     CDS             21041..21847
                     /locus_tag="IW651_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813726.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            22079..22948
                     /locus_tag="IW651_000981"
     CDS             22079..22948
                     /locus_tag="IW651_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0813727.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            22968..23315
                     /locus_tag="IW651_000982"
     CDS             22968..23315
                     /locus_tag="IW651_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813728.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            23328..24590
                     /locus_tag="IW651_000983"
     CDS             23328..24590
                     /locus_tag="IW651_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813729.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWATKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            24670..26046
                     /locus_tag="IW651_000984"
     CDS             24670..26046
                     /locus_tag="IW651_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0813730.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            26043..26672
                     /locus_tag="IW651_000985"
     CDS             26043..26672
                     /locus_tag="IW651_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813731.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRIQINKFEKKVRK
                     FNDLKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            26691..27170
                     /locus_tag="IW651_000986"
     CDS             26691..27170
                     /locus_tag="IW651_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813732.1"
                     /translation="MLELFEVNRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRHKKYVLLEYRHKITDEIGVNKTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            27672..27725
                     /locus_tag="IW651_000987"
                     /pseudo
     CDS             27672..27725
                     /locus_tag="IW651_000987"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27729..28328
                     /locus_tag="IW651_000988"
     CDS             27729..28328
                     /locus_tag="IW651_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0813733.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFTLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            28344..28820
                     /locus_tag="IW651_000989"
     CDS             28344..28820
                     /locus_tag="IW651_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813734.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGMKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            28837..29739
                     /locus_tag="IW651_000990"
     CDS             28837..29739
                     /locus_tag="IW651_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813735.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29812..30003
                     /locus_tag="IW651_000991"
     CDS             29812..30003
                     /locus_tag="IW651_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813736.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            30167..30754
                     /locus_tag="IW651_000992"
     CDS             30167..30754
                     /locus_tag="IW651_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813737.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            30754..31653
                     /locus_tag="IW651_000993"
     CDS             30754..31653
                     /locus_tag="IW651_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ0813738.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVSDATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYSDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            31653..32567
                     /locus_tag="IW651_000994"
     CDS             31653..32567
                     /locus_tag="IW651_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813739.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLGERKEQEKLIKAYFKLGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNAMNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            32619..32834
                     /locus_tag="IW651_000995"
     CDS             32619..32834
                     /locus_tag="IW651_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813740.1"
                     /translation="MKRDVFEFYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            33078..33278
                     /locus_tag="IW651_000996"
     CDS             33078..33278
                     /locus_tag="IW651_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ0813741.1"
                     /translation="MGIMFFDRISLKMDLSIAHQEFNLRLKALLNSDDVNFAHDVVGI
                     QNHIDRESKRMGDEFLPRYSSL"
     gene            33457..34500
                     /locus_tag="IW651_000997"
     CDS             33457..34500
                     /locus_tag="IW651_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ0813742.1"
                     /translation="MKKILVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPAPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            34672..34899
                     /locus_tag="IW651_000998"
     CDS             34672..34899
                     /locus_tag="IW651_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813743.1"
                     /translation="MNQRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGSK
                     RYEKAEESVDQLEEAVDGLSDILGLIEDITS"
     gene            34902..35159
                     /locus_tag="IW651_000999"
     CDS             34902..35159
                     /locus_tag="IW651_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813744.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSDKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLQSYTTSEKDK"
     gene            35261..35614
                     /locus_tag="IW651_001000"
     CDS             35261..35614
                     /locus_tag="IW651_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813745.1"
                     /translation="MKSENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVDFVKPHSIRRVQRGRKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEENFNEFFNIKSK"
     gene            35929..36723
                     /locus_tag="IW651_001001"
     CDS             35929..36723
                     /locus_tag="IW651_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813746.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKRRGLIPK
                     KKKGIWREFFKEEDSNRNNGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNIL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMIINNFTDLLAFAKKARDLRETQ
                     MYLFRNKNGRNRRTESSDIEDYYDAFTHDLGAFVDEISGVMDKYRV"
     gene            36752..37870
                     /locus_tag="IW651_001002"
     CDS             36752..37870
                     /locus_tag="IW651_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813747.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHYLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSK
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKTLREGEVNTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSEYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSLGKSKDEVKGVVIDDDFEFDFD"
     gene            37903..38223
                     /locus_tag="IW651_001003"
     CDS             37903..38223
                     /locus_tag="IW651_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813748.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            38549..>38792
                     /locus_tag="IW651_001004"
     CDS             38549..>38792
                     /locus_tag="IW651_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ0813749.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKIYHITNVSRATDLPYPLPKE"
BASE COUNT        14575 a         5277 c         7700 g        11240 t
ORIGIN      
        1 ataactaatt agtttaattt gaatgtaaat atacgctata aaagtgcatc ttgtgttata
       61 aaaaattatt ttattttgtg aacataattt aacaattctt tctcggaatt gatttctttt
      121 cttagatagt cttcagactt attaataata ttatctatta tgtctagata catatcactt
      181 ttcgattcag ctaattcata cagttttttt aattctttat cctttaattg aaaaccctga
      241 tccatagtca ccgcatttgg atatagaagc ccactataat gtttacgtaa atccacttgg
      301 aatttctgat tataaaagtc taacagatta gaaataaaag gcggaaaatt accgtttttg
      361 taaaattgat tatttccatt taggttgtat aataatttgt taaattgctc ctgatttaat
      421 ttattgtaaa tccatggtaa tgcttccata tccttatctg ctaacctgaa tgaactagca
      481 tcggtaagat gttcgatcat aaaatctatg aacatctctt tttcaccaaa atataaatat
      541 tgattatata ttgtttctaa tgttgaagat ggtagcgaag aaactctcat tttttgctga
      601 agtataggat caactagact atttaagtga ttttgtaatt cttcattgtt gtttgctaga
      661 actgcaagag aacgtaaacg gtagtaatat tctatttctg ttagtacttg ttttttcggt
      721 ttaaaatcat ttattgatat ttgtaaattt tgaattgtct cgtttgaaaa atcatgttgt
      781 aattctggat gggtagctat aaaagtaagt aaaaagaacc cttttgtata cgtgatctgt
      841 tgattttcac caagtgattt attaatttcc tctcttgatt cagctgtgaa cctatacttt
      901 gaaatttctt tttttagaat atttaaatag aattctttat tatcaaaaat tagggcatta
      961 agtagtctga aagtactttc cctatctcta tctgcttcct catttccata agataattga
     1021 aaaacaaact taaaggctgt cttacatagg ttttctttgg ctttttcgct tagtttattt
     1081 agatagctat tttttagtgc agagtgaaat tcttcaggtg taacagacat agcatgtatg
     1141 cgacttgcat attgtataat ttcgatcgtt actttttttc ctaacattgg attttttagg
     1201 aatataattt cataggactt ccgtataaca tcctgacaag tttcttttgt tatatctctt
     1261 aattcccaag tattaccgtt gtaagtgact acaggatgag ctgaataatt tctttgatcc
     1321 ttaatatatt ttaaatcctc atattcatag ctatcaatta gctcagttga actataagct
     1381 ttttctagta gttccatttc ccattttgga gagtttggat cactttttct taatctagct
     1441 atatcttcta aaattttctt agaagtatta tcctcgtaaa tatttgctaa atcagataat
     1501 ttatcaatta aatctaaaat aagcgcactg taattgacag ctattgcaga acgataattt
     1561 ccctgataaa aactacttct aacttcttct aataatggaa taattctagg atttttaact
     1621 ttttttattt catcatctaa tataaacata taaatctcct ttttttattt gtatgacaag
     1681 tttaccataa aaaaaaaata aaaagcgaga catatttatg ttattatgtt atcataataa
     1741 cataaatata tggagtgaga tgatgaaaaa atggatttga aacaatcgtt ttacatctgg
     1801 ttatatcctg ataatcctat gtcaataatg gctgatattt ttgtagttag catcttattg
     1861 tatttatttt atctaatttt ctttcacaat ttaatcgggt acatgattct tgattggaaa
     1921 ggaatgttcg atatagagag aaaaccattt gctaacaatc aaacctatac cgtcttcgaa
     1981 agtggagatt gggaagaaaa tgaagttgat gtgatccatg aaattggatt agctttatca
     2041 ggaatacaag atgctgatgt tttggtatca atgacaacgg atactggata caacgcttat
     2101 gaggtatttt atcttatgta ttttatatgt cgtgatggtc aagaaattat caatggtcgt
     2161 gctgaaaaaa caatcatttc taatactgaa atttctttct gtatgggtga aggggaaatg
     2221 attgtatata aagatgggtc agcaaacatt aaaacagcct ttggtgatat tgatacaagt
     2281 attaaggaac ttagggaata tgtgcagaac ccatataatt ttcaatattt agcaggtaga
     2341 attaatttag tttaggagta aaaatatgaa aaaaattatt aatcaaccaa caaatcactt
     2401 gataattgaa ggtgttattt tcgtaacaat gtattttact gtaggaaatg gtatctcaaa
     2461 tacaatcatt caaaaaaatc cacaatactt attatttagt ctttatgctt ggattgttca
     2521 aatggtttgg gtttctatat tgatgttgtt gttttattgg atagtacggg ttattctttt
     2581 agtgttaaac aaaagaaaaa aggataggtg aataaaatga aatcaactaa attgaaggtt
     2641 gtattgttgt caacagcagc attaataact cttacagcat gtggtacaac taatagtgat
     2701 aataataaca aagatacaaa gtctagtaat acggtggtta ctaaaaacag cactacatcg
     2761 gcatcaacaa caatgtcttc aatgattgag aataatgctg aaaagtatca aaaatacgaa
     2821 gacattaata aaggtcaaaa gccggaagaa gtttataccg actttattaa tacagtaaaa
     2881 aatgatgaag attatgggaa tgatccattg caaaataaaa atctgactat cgttctttac
     2941 aaacctgatt gtccagattg tcaaaaagca gaatcaaaaa tcatgcagga aataaaagaa
     3001 ggctttagtt atgctccaac attaatgcat gatattacca taaataaacc agcttacgag
     3061 tatcacgcag ttgctgtaga tattactgat ggtgtacctg agtgggtaca aaacattcat
     3121 ggtctggctg atgagaacaa tcaatatcat acgccaacag ttgcagtagt aacaccaaag
     3181 aaagttaatg gcacactata ctgggatgta atgggaatga caacaaatac aactgatgaa
     3241 atgattgaaa gtacaactaa atattatatg gatttgggag catataaatt atatcaacaa
     3301 tcagaacctg ttatggacga tcaatccaaa gttaaatagt tctttagggc gtaagcccta
     3361 tttttttaaa attaagtgat aacaaataaa tgatttctga aacaaacgtt cttactcaac
     3421 tactcgaaac acacaagcta attaactaga ttataaagga gaatgcgtaa tgaaagaaat
     3481 cagtgcaaat gaagctcata ttccgcaaaa gaatcaggtt ggtatatata aggtgcttca
     3541 tggtgaagga atttataaaa tacagttact taccggttac aataacggtg caaatgggaa
     3601 gtggttaaag gcaaaaagta ttcgtgaact taaaaatgaa gtagaaaaaa taagacttgg
     3661 gttaacaagg gaagaaataa gtagactgag tgaaaattct tcttttgtaa tccaaaaaga
     3721 aaaggagaaa catctacttc atcgggaact aataattgaa aaatactacg atgttgattt
     3781 acctgatcag ttaagaatta ttgacgaatt ttcagagaaa aagtggaatt ctgaaatata
     3841 cccaagctta attcaataca aggcatcagg aaagcccatg tataactaca caggtttttt
     3901 aaactataca caatataaat atgcctttcg caaattaaaa ggcattaaac aagctgacat
     3961 tgccctgtat gagatgaaaa aaatggatag atcaaaattg attaaagagt tagtgcaaaa
     4021 tacatgggat caagcgattg tcgaactaga aaaagaaaac ggaaaaattt tagctaataa
     4081 atacctaatc gtaaatgaaa ttgaagaatt aataggttta ttttcatgta aaaaaggtga
     4141 agttttctta aaatcattac caacttattc aattttagac agtccgtatc agcttataca
     4201 ggcaaggaga tgagttaaat gtctagcttg aaagatgatt atatccaagc aaaacaagag
     4261 tatgaaaaat taaatatgtt gaaatctgaa aaagtcgaat tgtttttgac tcaaaaatta
     4321 gcagaggagc atatttctga aatagatgca aaaactttag agaggtatga ccagcagttt
     4381 aatcagatac atcacaacat cattttgctt gttgatcagg caaagagaag acttgaaaaa
     4441 gcagaatatg atttattaga tcaaggaatt gtatgcttgg ataaggggat acgaagatat
     4501 tatagtgcag agttaaaaaa gcaaaatgta gatagaaatg cactaaaaaa tgaaatttta
     4561 gaattgataa tgcaacgaat ttgaagggat agaaagcaaa tgtcacaaaa cattagtgct
     4621 gtaaaacaat taatagaaga agatttagag actacagttc agagattagc ggaacgaata
     4681 cattttgcaa attatcaaac catctctaac tggtcaagca ggaacagaaa tccggaagaa
     4741 atcccaatga aattttttta tcgaatcgga aaagaatatg gtgttgatcc gataaaaatt
     4801 ttcgagaaga ccatgatgta ttactatcat ggaacaccta acgaagtgtt agaacatctt
     4861 ttggaagaag caaaagaaaa atcatcaata ttagagctat cgcaaaatga aaaagttatt
     4921 caagtgaaat gcgttgatcc aacaggaaag attattgtcg gttttgatat tgaaagaaaa
     4981 tctaaaatag attttattac tcaattaaaa aagataaata atttagatat tctaaccctt
     5041 agaccagatc atgaacagtt gtttatgagt tttaaaaaac aattgaaaga aatacaaaga
     5101 agatataaca aggtgtttcc taatgcgtga aaagctcaaa gaaatcagag tgttttggat
     5161 aattaacaaa gaatttatca tcttatatac tcttggattt ctcagcatga tcttgttttg
     5221 gtggttatat ttaaaaaaat aaaaagaagg aagaaaatat ggcatacgaa aatttacaat
     5281 tagtagttgc aggagttagt ggggatatat accttgcaaa agcaaaagat ggaattatgg
     5341 acacgaacta taggagagtt gctacagatg atgtgttaag ttcagcgaca gaatggtttc
     5401 gaaaaaatga agaatctgct gttcactttg aaggaattga tggaaacact catagtttgt
     5461 tctatacaaa agacaaagaa aaagcaaaaa aaataattga tattttgaaa gaacgttagt
     5521 taattaacca acgatataag agaaggtgag accaagtatt tgcttggtct ctatttgata
     5581 gaatacatat tctatttaaa aaaagaaaat cattgatgtt gtacacatta gtgtgtataa
     5641 tcgagagagg ggtgattaga actttgagca attcaatcat tgctttcgta ggagaataat
     5701 agaacagttc ctaagaagtc cattgttcag taaatttttg agtaaaggag acgttatgag
     5761 caaatttgaa tttttagata aaagtattgg ttctgctttt ttaattgatc ctgatctgtt
     5821 tggatcatgg gcgcaatcat caggttataa ttgggctttt agtgatgata tggtcgagct
     5881 tttaagtcaa acgatgaact gggaagctta taagatcaag cgatttttag atgttgttgc
     5941 acagccgatt tatatatctg ttgatgatca ttggtcaggt agagagttga caacactaaa
     6001 agaactagcg gagtacaaca aaagagacaa tatcattcaa gtagctttac ttgaaactaa
     6061 gggaagatta gaaaatactc gatggagtat gataaaacca tcagcactca gatttcatcc
     6121 taaagacttg acagcggttg tagcggtaaa agaggttcaa gactcacaag aatactatga
     6181 agtagtaaaa aatctgaagg atatctggtg taaataattc taaattgacc agttatttgg
     6241 tcaaaaatag tatgtgatta tttcatccaa tatggcatca taattgggtt aaaaatgtta
     6301 aaattcaaaa aaatgagtca gaagggataa aagtatgaaa agagaggaat acaaacaaag
     6361 attaaacgaa ctgcttgaag aggacgaaac attaactcat ggttcgcctg atgaaattct
     6421 atacatgatt gataatatgg tgatctttgg tggatatgaa cttggtaata ggtcagttga
     6481 tcataatatt ttagaatttg atgatgtatc atgggaagaa attcttgatt ggggaatttt
     6541 ggcggtacct gaaactaaaa tctatattag tgacactatg gttccttttt ttgaggaatt
     6601 ggattataaa agacttccca aaaatgaaaa tcatatactg ggaggaaact aacaatgtta
     6661 agtgacttat ttattttact gatctatcta ggtgtaagtg aaattattgc gtattttttg
     6721 tttgtcattg ctaattatgg tatttccagt gcaatctttc aaaggtttag attaccagta
     6781 agtgaactaa aaaaatgatg agattatcag catatctttt aatgtttttg aatgtggtca
     6841 cgttaatctt tgtagtaatc ggtgtgactt tcaatttaat ggaatagagg gaaatctata
     6901 atggaaaaaa gagaaaagaa tgaacatatt ttttgaatca atattgttga tggtttttgc
     6961 gatcagcgct taccagaaat ctaaaaaaag agtaatgatt gtttaatcat cactcttttt
     7021 ttgtagggaa attgaaatat gtggagcttg tattggttta gttaggattg cttggctata
     7081 aggagctagt tctttatcaa aaccatcaaa gtaggttttc agtagtaatt taactgatcg
     7141 ctcatcttgt ttgatttgga taatttcagc atccaaatcg gttgtgagat caggagcttt
     7201 tgatgcgatt acctgatttt ttttatcttc caactctctt aggctgttta acaagttgta
     7261 aaaagaatca ttttgattgt tcattccaac gtgagttgca attttaacag aagtttcgtc
     7321 cgtattgtca tcaaaagaac tcattggaat atcaagtagt aacgaggatg tttcttttac
     7381 tgactttttc tttatagcat tttttgtttt tccatctttt ttgctcttta ctcgagatgt
     7441 ttttggagct agtttaggta aatcacttgt tttatcagtc atttattagc ttttcctttc
     7501 tattaattta tgttattata ttatcataat aacataaaaa agtacacttt ttgggggcgt
     7561 agttattgaa aaaatctaag agaaagattg ccacgttgct tatgttaatg gtgcaattta
     7621 cagggattgc aatgagtgta atcacaataa atccgcaagt gatacatgct gatgggatag
     7681 ctacttatga tgcacaattt aactgtacta ctgaaggtag tgggtcagga acagatagcg
     7741 atggaaacga tactggaagt ggcgctgtaa caggtgatgt agccaaaaat attgaagata
     7801 tttacaaata catgaatgga gaatatggct tatcaggtgc tagtattgca ggtattttag
     7861 gtaactggaa acaagaatca ggtattgatc ctcgagcagt agaagggtat tacggagatg
     7921 gacagagaga agttgacttc atcaaacaat ttactgatgg tctgatgggt attggctatg
     7981 gtcaatggac agctgggcga catacagcat tagttgattg gtctaagaaa aataatggcg
     8041 gttcttggtg gactacagaa gcccaattgg actttatgtt caaaggtgat gggggattcg
     8101 taggaatatt aaaagattat gctttaaatg cttctgatga tgtcgtagaa aatgcagtaa
     8161 cttttcataa aaactgggaa atttcagctg attccgaatc agctgtaata gctgtacgtg
     8221 gtgaaaatgc aaaagaaatc tggaagtaca tgaaaagcaa gggtatggac ggtgcaaaag
     8281 atgaaagtaa gatcaataaa atatccggag gttcaagtac cggagatgga tcgaataatt
     8341 catcaagttc agcatcagga acagtagaag atgcctgtgc aactacggat gacgctaatt
     8401 ctggttctgg atcaattggg aaagaaggaa aaattaacgg taaaaaaggt caagtaatag
     8461 cgaatatgac ttatgaagag gctttgaaaa aatatgggaa agcgatcacg ctaccaaaat
     8521 tcaaagaacc agattggtca acttcacctt ttaacggaat atatcaaggt cagtgtacag
     8581 agttgacgtg ggcttatatg agccaattat acgatgggaa tcaaccgact actggaaatg
     8641 gtggctatgt atgggaaagc tacaaatcgg caggagcaaa aataacgaac aaaccaatcg
     8701 taggttatgg gtttagtgct tcggatggat ggttatgggc tgttggagat gcagggcata
     8761 ctggtgtagt tatagcggtg tttgaagacg gatcatttct atgtgctaac tttaatgttc
     8821 caccaaccca agctccaaca aggggagttt cggtaacgtt gattgatgga gtggacggat
     8881 cagataatgt gcatttcttt agcggtgtag gaaaagctaa atttgaaatc gaaaaataaa
     8941 ttggaatgaa ggacgatata gatgaatagt aaagtgaaag atacaattat catttctttg
     9001 ctttctggta tgttattagc actgcttgtt ggaatcctat tcttcaataa gcagaatcag
     9061 gacatagaag ctgaaaaaca cagtgtgcat attgaagata agctgacatt ctttaaagag
     9121 gttaaaccaa atgaggtgga tggattacgg aaagatttag aaaatgatat tcaagaaatg
     9181 ttgaatggat cagttcaaaa caagtttgca gatcaatcag gtgaagaatt tttgagaaaa
     9241 ctatttattg tcacaggtaa accaccaatt actgataaga gtagtgagaa agaaaaaatc
     9301 gagtactata agccttttac ttttaagcta acaaactttg gaattaagac atctagtagt
     9361 gggttgtatg aagcattgac cgattttgaa gttcaataca atggaaaaaa agtgtatgat
     9421 accacattaa ccatcacact aaataatgat aagaaagtga ctgggggtgc tttttatgca
     9481 aaatcatgaa atcactactg ctaaaaaggc gatcggaata ctttccataa ttgtgatcct
     9541 ttgttgtgtg gtactatttc aaaagaaaaa tgcttatgat aataatgtca aagaattgga
     9601 agaccttaaa agtaaacaaa tcgaagtagt caagaataac cagaaagtga aagctatgca
     9661 ggaagctcaa tacaaagaat tgggaaatgc tgaaatcaag gaacaaagta ggaaattctt
     9721 taatttattt tacaactgga attcatggga aaaatatacg aagaatatga aggaaataca
     9781 aaaagaattt cctcagatag taaagtctag tcctgttgat atttctggca cgatggtagg
     9841 cactggtaat agtccaatat ctagctatac tgctgattac tatacaacaa atcaaaaagg
     9901 taaaatggtt gaattgatta cacagacaag aaatactcca acaacagcaa caacaacaat
     9961 ttggcgtgga gtaacaagca taggagaaaa tcaattgttt tacttggagg atttagatag
    10021 ctatgtaaga gcagagtaaa ataaaaaaag agctaacggt accaacgtta gctctttgtg
    10081 ctgacgatga taacaaaacc taccgaaaaa ggattgtttc atcacaaaca caaacagaaa
    10141 agaattgata aaaaacccga acctcagaat ttttattaat caattcattt agattataat
    10201 aaattgagtt gaacattaca atacgtatat gttgtaaaac gtaaaaaaag tcaaaagtaa
    10261 atactttctt aaaaagtcaa ataaaactat taaagacaat atatatggtt tatttgacat
    10321 aagaaaatgg tctagatgat aattgtaata tagaattgta gacttaaata aagcataaaa
    10381 taccttttat gataaggtat agctcttaat tttaagcagg tttagatata agattaaaag
    10441 taaagtggag gagaataata atggcagatc gtaaattaga aaagttatta gaagaaacgt
    10501 ggaatccaaa agaatttagt gaatttttta tggagaattt tgaaactgac ttagcagtga
    10561 ttgttaaaga tgctcttaga gagcaagggt atcctgaaac tgcaaactat ataaacatta
    10621 attttacttt gtatactgaa aataaaggaa cttgggattt ttgggctact ttagcaaata
    10681 aggagttaag tgataaatca gacacaggaa ttcgtaactt ttttgaatca aacagagatg
    10741 attatatgta tgctaacaat caaaataaat tgaactttag agtagagttt gatgaatcac
    10801 ctgaagaatt tatagaaaga caaccgccta aagaaaatgt agcaaaggta ttggaagatc
    10861 gttggaattc agatgaaata gtttcaacaa ttagcgaact aggcggacaa tatgaaccgc
    10921 tagtagaagc ggttagagaa gagctaagac tgaataagtt tcctgatgtt caaaatattg
    10981 atgtttctca aattgaaata aacgtcaaga tcacgaataa acttgattat ggtagttggg
    11041 cagatatagc attagaaaaa tacatctact caacgttaaa agagtttatc gaaaatagaa
    11101 tggatatcat gtatctacag catccacagt atttaaactt tggtgttgaa atagctacac
    11161 ctcttgaaga atggaaaatg gagcaagggc ttgactagcc tttgctccat ttttctattt
    11221 tacaaatgaa tacatttcat catttttacc aagaatgatt gtttgcccaa ctttgagatt
    11281 gtcaggtgat ccttcagcgg tttgtaagga gaacgttcca ttgaatttat cggataccgt
    11341 tataggtagc tttaattctg atccttcctc atctttgaga attgctgttt tccgttcatc
    11401 aaaataaaca gtctttatta aataattttt cgtagtgatc tcagcagatt tgtctttttt
    11461 tgtagtagta ccagtttctt tagaattaga ggtattttct acgattattc catctttggc
    11521 atttgagctt gtgtttattt gattgaatat tttcagcgac aagataccgc taagaatagt
    11581 caatatagaa acaagtgtaa ttaatactga taatttcctt tttttgttca tttttcttaa
    11641 cttccttttt tgttttgatt agttagatat tccttatcta acaagcattt ttaggctatt
    11701 aaatgaagat aaaccccaaa aaaatattgc aattatgaca gtgcaatggt actattatta
    11761 tacaataaat tttgcgaaag gggtaggtct aaatcgcaaa caaaaagaaa ttgaacctag
    11821 acaaatacaa cttgtatacc gcaagggaag caagtttgat tttgggtaaa ggtagaaatt
    11881 atgttacaaa cataatcaac tattatccaa atcgtttgcc taaaggttcg ttcaagaaag
    11941 tgggtgacaa aacctatatt ataacggaag aaggaataga agcattgcga tcacaaacag
    12001 aaaataaaaa caaaaagaag gaatgaatat gaagaaaaat tcgcttggta tggttttatt
    12061 tgcatcagct gtacttgcgg ggttatcagt accagtaacc tcacatgctg atgaatggac
    12121 tgcaaacaca ccggatcaaa tccaaattac aacaggacaa tcaagttatg cgttcaaaaa
    12181 aggagatact atttggtcaa ctgtagagaa gcttaaaagc ttaggttaca agactactgt
    12241 tgctgagttt gccgaattga acaatgttga tttggcaaaa ggagaacaac ggactatccc
    12301 aattggacga actttcaaca tcagtaaaga tacattaaca gttaaggaag cagatggcga
    12361 agtagttagt caaactgttc aaaaggataa acctgatcct gttgaaaata cggagcaaga
    12421 gaacaaaaaa tccgagacta caaaagaatc ggaaccaaca gcaaacactt ctgataagac
    12481 agaaacaagt gcttcaagtg ttgatggagc aagttcatct aatacaaata attctacaag
    12541 tgagaaagaa gcatcacagg gaacaagtga aagtagcact acggaagctg atactggaac
    12601 atcaacaaat aactcacaat caacatcaaa tgatgcaaat gaatcaacca gtacatcttc
    12661 tagtgatcaa tcacaaacag gttcgagtga tgaaactgac agttctactt caccaagtaa
    12721 acccgaacaa ccaactaatg ttgtgctagg cgttccattt attagtcaag gtaacacaat
    12781 gctttgcgaa ggaacatctt tgctagaagc gttacattat aaaggtgttg caacaaatca
    12841 agatttaatg acttttgtta aatcaatgcc actatctcct gataataatc caaataatgg
    12901 tttttctggt gaatggagac acaatgtgga tggaacttat caaggtatga atccggctcc
    12961 tgtagtttca tggggtattg ctaatggcgg aaatgttgta gatatttctg gttcatcggt
    13021 acaaggatta aaagatgaga ttgctaatgg aaaccctgta gtagcgtggg taacttatgc
    13081 ctttgaacct gctcaacata aatcaatgtt ctggggcgat gctatttgga atagacatgt
    13141 agtagccgtg gatggattta aagacggttt ttaccatgtt gtcgatcctg tgtttggggc
    13201 aagttggatc aattcaggat catttgaaac agcatataac gtatctcaat tagcggtttc
    13261 agtacgttaa taaaagagat cgtttgtata ctaagtagtt aagtaaagta tttgtttgtc
    13321 attgcttaac tacttagtaa cttgttgctc acggtgggct agaactaaaa ggggtgatgc
    13381 tatgaataat gaagcgttga aacagctttc tttcaacgac aaaagtgatt aataggtatg
    13441 gtgattttag cttaggaatg ggcgcgaagt cattaccaca ccgacatatc gtgactcatt
    13501 ctcttcgcct aactggtata aagaaggagt aacgagagat gaagcatgaa aaaacgataa
    13561 ttttaaaatc aaaaataatt tattgttttg cgactttagt aatggtgtta tctacgctta
    13621 taatgccttt ttcagcaata agagctgaag cagatgtcac aaaaccgaca agtgcaaaat
    13681 ttacagaaac tggcgtaaaa acagacggtt atattagggt taatatgccg aatcatccaa
    13741 atgaatggat gatttcaagt caatttaaag attctcatgg aaatattgga tattgtatgg
    13801 attcagaatt gccatcccca acaggaagtg gagccggaag cttgaagtac aagggagctg
    13861 gcagtgatga gttctatcgt atgtttaaag gtggttttcc atcaaaaaca gcaaaggaac
    13921 tgggtgcagg aaatgataca gaagcatggt atgcaacaca attagtttca tgggttttag
    13981 ccggaaactt taaagtttca cagattgtat ggtcacatcc aaaccatacc gctgcagaaa
    14041 cagctcgtgt aaaaaaagca tttgaaaaaa tttatgacta tgctaagaat ggaaaagata
    14101 cgcccaacac tgagttttct atcacagctt ctaagacagc tgacgaagga aaatatcaca
    14161 ctttcacata taaaacagca tcaaataaga ctggtaatgc gaagctcact tttacatcag
    14221 caaaacctgc tggtatgaag atttatgatg cagatggtaa agaaattact aacaacactg
    14281 taaaattaaa ttcaagtttt actattaaag tacctgtgac tacaccaagt ggtactttgt
    14341 catttaaagg tactgctaat gttagcacaa ccaatccttt tacttttgat ggacgaggtg
    14401 tgtatcaaga tgccgtagtt atgatcacta cttcagaaac aaaggactct aaatctttga
    14461 gtgctaaatg gacaagagct gttggtcagg taacagttaa gaaagttgat caagatggaa
    14521 atgctttaaa aggagcggag ttcactctaa ctgacaataa caatgctaaa tctgttaaga
    14581 caaccggaga tgatgggtta gctacgtttg atttgcaaca aggcttaaca tacagcttag
    14641 aagaaacaaa agttcctgaa ggttatcaag gttcatttaa aaaggatggt attactgtta
    14701 ataccgatgg acaagaattt agttacactg ctacgaacac aattaaaaaa ggtaaaataa
    14761 aagttaaaaa ggttgaccaa gatggaaaac cattagctaa tgctgaattt accttgacgg
    14821 atgattcatc ccaaacacaa gcagaaaaaa cagatgaaaa cggacttgca agcttcgata
    14881 ttgtagcaaa caagacctac agccttgaag agaccggagt accaactggt tatacaggcg
    14941 cattcaagca agaaggaatt accgttaaag atgatggaca aacttttgaa tatacagcta
    15001 aaaacacttt gaaaaaaggt gtagtaaaag ttcataaagt cgatcaagat ggaaaacctt
    15061 tggaaaatgc agagttcact ttaaccgatg aaaaaggtgc aacacaagta gaaaaaacag
    15121 atgaaaatgg acttgcaagc ttcaatattg tagcaaacaa gacgtatagc ctagaagaaa
    15181 ccggagtacc agtaggttac acaggtgcat ttaagcaaga gggtatcact gttaaagacg
    15241 atggtcaaac ttttgaatat acagctacaa acactttgaa aaaaggtgta gtaaaagttc
    15301 ataaagtcga tcaagatgga aaacctttgg caaatgcaga gttcacttta accgatgaaa
    15361 aaggtgaaaa agaagttaaa acaacagatg aagctggttt ggctactttt aatatcgtag
    15421 caaacagaac ctacagctta gaagaaacaa aggtaccaac tggttacaca ggatcattca
    15481 aacaagaaga tattactatc aaagatgacg gacaagtttt tgaatatact gctaccaaca
    15541 ctttgaaaaa aggagtaata aaagttcata aagtggatga tcaaggtaaa gcattagcaa
    15601 acgcagaatt cactttaacg gatgatcaag gcgaaaaagt tgttcaaaag actgacaaag
    15661 atggtttagc gacatttaat attgttgcaa ataaaacata caaaattgaa gaaacacaaa
    15721 atcctaatgg ttatacagga aaatggtcta aagaaggcat cactgttgtt gacgatggac
    15781 aaacttttga atatacggta attaaccacc gtgtacctga aacagtccgt acaggcggga
    15841 tcggtacatc agcttttctt cttgtatttc tagttgcagg attaggcatg ggattgctgt
    15901 attatcgaga acaacgcaaa gtaaaataaa aaagcggtaa tggaaaagaa gtaaatttga
    15961 aaaaagaagg ctgacgtatg aaatcaaaaa aagttttttc taatttaatg ctatttggta
    16021 cattgctagg agtgggattg ggcgcaagct caatctcttc tagttttgga cttggggagc
    16081 aagtagcata tgcacaacaa aactataatt atcaaggaac tggaactatt catattcata
    16141 aagtagcaaa tactaatggg gatgggaaac ctgtctcaga tattgacaac aaaggaaaag
    16201 aaatagcttt accaaatggt gtacaacctc ttgaaggcgt tgagttctct tatgtgaaac
    16261 ttactaaaga tgaagctaaa tccttgggat ggacagatag tgatactaca aaaggtcaac
    16321 atattgatga tgctaaagca cgattattac taaacaaaaa atacagtgaa gtaagtagaa
    16381 tgaagactga ttcacaaggg aatatagaca tacctgtaca agtaaataca gacgatttaa
    16441 cagataactt gtatttgatt gttgaaacag gaacagtcaa aggtgtagtt gaagaagcgt
    16501 atcctatgat tgttaacgta cctgaactaa acggatcaga tgatcaatac tttaattatg
    16561 atgtacatgt ctatcctaaa aactatttcc aattaaactc agcagaactt gataaaaaag
    16621 acgaagatgg aaaggctctt gccggaactg tttttgaatt gcgaaaagat gatgggtctg
    16681 atcaggtcgt tggaacttat aaaacagata gcactggtaa attaactgta gacggtttgg
    16741 aattaggtga ctattatttc ctagaaacag atttgggaac ggatgctaat aaaacaacct
    16801 atttactaga tggaaaaaaa ttcaaattta gcgttaaacg tggggagact gctaaagtag
    16861 ttcttgatgt taccaactat aaacgtcctg taattactaa ggtgattgat aaagaaactg
    16921 ttgatattgg tgaagatgtt cagtatacat tgaaaccatc tattcctggt aatattggag
    16981 attacgaaaa atacgttgtc aaagatgtac taggagaaga gttgacttat gattcttcag
    17041 aagtgataga aggaatgact ttaaatactg actacagcat cgagcgtgac ggacaaacga
    17101 caaactatgt ctttactgat tctggtatgg ataaacttca gaaaaaattt gatgcagggg
    17161 atcgcttctt tacaatcttg atgaatgctc acacgaatag taaagctaaa atggatacac
    17221 cacttaaaaa tgacgtttca acaatctaca acaatggata tgagcctgaa gacgaaacgc
    17281 cagttgatca tgcttataca atcattggtg atcaccaatt cattaaaaca gatggggata
    17341 caagcaaagc gttgagtgga gcgaaattcg tagtgaagaa caaaattgat cctaacagag
    17401 ttacttacct gaaacaagac ccaacaacga aagctattac ttgggttgaa gataaatcga
    17461 aagctactga attcgtatct ggtacagacg gtaagttcca agttgaaggt ttgcaatata
    17521 cctatgatgt agattttgat aaccaaggta atccaacaat tacagataaa aaaatcaatg
    17581 attatgctct tgaagaagtt gttgctcctt ctggatatac accaacacga aatgatgttc
    17641 catttgtagt atctaatgat gatgcttcaa gtaaagtgac agacataaaa aacctgaaaa
    17701 ctcctgaaac agttcgtaca ggaggaattg gaacagtagt attcctagtg gttgcaggtg
    17761 tcgcaggaac agggttgtat gttgtttaca aacgggaaca acataaagct taattattaa
    17821 aaatccccta gagtgttttt ggctctaggg tttcttttta aaaagagtat gaaaagtagg
    17881 tatgagtatg aaaaaaaaga atatcaagct aatcatctta agaattggga tgttgctatg
    17941 tctaggagta ggtctatacc ctgtaattac tcaggcttat acctattacc agactcaaca
    18001 aacgttaaat gcctataagg aaaaatatga tgaagcttct gaaaaggaga atgaagaaat
    18061 cttcaaaaaa tattcggaaa tcaataggaa acgaaaagaa acagaacaaa ccttagttac
    18121 gattcctgaa gataataaca atattccgtc aaatggaaat cttcaaaagg aaaaattggg
    18181 tgaattagta ggtgagattc ttataccaaa aataaatgtg gatttaccga tttataatgg
    18241 cgtaaacgat tttcaattat cagcaggagc aggaatactt actggaactg attatccgac
    18301 cggtgggatt ggaaaccatt ctgtcatatc agctcattca ggtattccta ataaaaaata
    18361 ttttacagat ttagaattgc tcaaacttgg tgataaattc tttatcaatg ttgcaggaaa
    18421 aaaacttgct tataaagtag atcggataaa aataattcta cctaccgaat ttgaggattt
    18481 gcttcctgta gcagggaaag attatgtaac gcttatgacg tgtactcctg ttggagaaaa
    18541 ttctcatagg ttacttgtta gaggagttag aataccctac aaaaaaaata ttgttgagaa
    18601 agaaaaaaag aaagaagact taaaagtaaa ggcgcagata aactggtatc ccatcatagt
    18661 tgtaggtgtt ttatcaataa tagctttaat agtcattatt aaaaaaatag tggcaaagag
    18721 aagagggatg aaaaatgaag aataaaatac tacttttttt gactcttatt tgcactttgg
    18781 ttttaacaat ttatcctgca aatcagctct atcatcaatt taaaacgaaa gagaatgtgg
    18841 cggaaatcca agagaaaatt gagaaaaaac aaactccttc aaaagaggaa gtggcaaaaa
    18901 aagtcgaaca aataaaatcg aacagcggta accaaggatt agacaataat cagaaaacag
    18961 acggcggatc atttgatatt ggtgaagctg aaggaaaggt caatccttac acaggagatg
    19021 ttttttcaaa aaatcaaacg aaagattttg aaccatctac ctattcagag gcggttggtt
    19081 atgtctatat tccaaaaata aatgtgctag agcttttgtt tctaggcgct tcacaggagc
    19141 atttatatag aggtgtggca cagatgagtg ctactagtct acctttatca ggtaaaggtc
    19201 atcaaacagt aatagcaggt catcgtggcg gttatacgtc accacaattt ctttatgttg
    19261 atcaattagt agccggagac cacatttata tagattatct tggtgaaaaa gcagtttata
    19321 cggttttccg aacagacgtt attagaaatt acggtcagaa ttcaacactg actgatattc
    19381 ctgatttaga tatgttaaca ctttatactt gtcatccata tcctcagaac tacgatcgtt
    19441 tattagttag aagtctaaga gatgaaaaag caaaatacat cacgccacaa gtaacagttg
    19501 gcaataccgc atacgctgat gccattgata gcgagtccgt ttcagataag acagaaactg
    19561 ttacatcttc taatgattta tttgaggatg ttgatcgtgc caacaaggaa ttagatggaa
    19621 ataatgatct taatattccg gaacaaggta gcgtagagat gcaagacaga acgtttgctg
    19681 aaaaggcaag ttcaatattt acaggaatta ccaataaaca tcagaaaatg cttttagtca
    19741 tttggactgt aataggagca ttagcaagtc taaaggttct tattctttta atcaaagagt
    19801 ttcgaaaagc ataatgaaga ttataaaaat tgatagagta cagtaaaaag ggattgatac
    19861 tattattgat ctctttttta ttttgaatag agctagattc acttttaaat aaaatcaaaa
    19921 ctgatcttaa ttctaaagaa aaagtcaaga aaaaaataca acaaattttt ttatcagaat
    19981 atttatcaga atattattag aaaagataaa ctcaaaagct taatttaata gggttattgg
    20041 ttgtatttag aaataatgta tggaaaagct ataaatcaat agataagatg tagaaaatct
    20101 tatttgtgaa tgaaaaacaa gtcatcgtaa aatattaata ggaggtgtag taatggtggg
    20161 aatatcaaaa acagctttag actcattagc taaactattt agtagcaggt ttgatggtgc
    20221 agaatttgtt gaatatctaa gccaaaacga aagtggaaaa actctgatcg aaacaatgaa
    20281 aaaagactat ttgaatgaaa atgactttcc gacatggacg aatattgaag atgctacatg
    20341 ggatgtagta tgtacgatat attctccttg ggtaacgatt gctgattttg ttttagataa
    20401 aattggaggg gatacatacc ctgatgccaa atctcttgct acaagtgact ttaaaacgtt
    20461 ctatggttat ctaataggtc taataggcaa tggaatagat ttgaaagtta cgccaaaaca
    20521 aacacctgaa gaaattgaaa aagaaaaagg agaggttcaa atggcttatg caaatgacag
    20581 agaaaaagta aaattattgg aaggtaaatt atctgaagaa gaacttggaa aaatggtgca
    20641 agaaatcaat gaagacagca actattttcc tgaattgaag gtatatcaaa atgacgaaga
    20701 tttctttaat gaaatgttta gtaatagtcc atgggaagca gtgagtaaaa caaatttagg
    20761 agaatggaac tttcaagatg atctagttgc atttagcaat gatgggtatt tacgatcata
    20821 ttcagaaagt gagtatagag ctgaattaaa aacgtatgaa acggatattc tgatgagcta
    20881 tttaaatcta ttagagagtg gtcagggaga gttaagagat aaagttgaag aactattaaa
    20941 agaagagcta tagggattat ttcaacataa acacaagcag aaaactacta aacaaaacgt
    21001 accatgcaaa aagtcggtta aaccggcttt ttgctctatt atgagaggag atcaaaccat
    21061 gagtgcaaca accgatatgg ctttagaagc agtaataaaa gataatacag gaaatgtcga
    21121 aaacgtagaa gactttagcg atattgcaat caatctttca gcatggattt cagctgtagg
    21181 agaagaaaat atcgaatttg agatcaagaa tatggatgac ttaaaagcaa aatttccttt
    21241 tatcgcatcg aaactaagtc aagttgccga ggataaatcg tggaatgaat tagttagttt
    21301 ttccgaaaaa atttatgata ttacgcaaga agatgagtgt gtttcatggg aaatgaataa
    21361 tcaatacaag aaaattgacg ctttcgtcaa atcacgtata gaaggaacag cttttgagtt
    21421 tgaaggtata gataccgcag ataattggtt aaataaagaa tcctttatta aaagtatagc
    21481 ggatgatctt gaaaaacaat ggtctaagga agactatgca gagtacctaa aaacagaaag
    21541 agatacagag gtgaaagaaa aactcactga ggtatgtttg gatgaacata ttgatcccaa
    21601 tatcgtaaaa caagaagact atgaagtggt tattgataaa gttcctactg attttgagga
    21661 agttgtaaag tatctagttg aagaagcatt atcgcaatac tcttcagatg aggaagtgga
    21721 cgaagaaact gttcgtacca gagtttggca actggttttt gatgacaaag attataaata
    21781 tttgagtgat caaaactcgg ttaattggag tgtacgatta ttaattgatc aaaatgatat
    21841 tgaataaaat agagctgaaa agctctattt tttttgataa aaaaactagc tattttatta
    21901 aaaataattt ggttcaaatt ttattttttt aacttgataa tttcttgcca tcctttttta
    21961 aattggctat atatcaaggt ttatagtggg aataaataaa aatacatttg acaaaattta
    22021 caatatagtt tattatatat ttatcttatt atgataacac aataaaagga atggtggtat
    22081 gaaaaagaag aagtttagtc gatcgttgtt aggaattttg gttttagttg cggttggtct
    22141 tagtggcaag ggttactttc agaataacag cactcaggaa catgattttc agatcaaaag
    22201 tagtgttcag ataggtaata aagcagacag tgaatcaaaa gaatttttag ggatacgtta
    22261 tgatccaaac aaatatccaa ataatatcgt aaacattaat gatgggaaat atacactgac
    22321 aaaagaagac catgaagcat tatcagaaaa aggcagtcaa accttttggg taaattatgc
    22381 agatttagat caacttggaa gagcaggaga tgttgaagcg ttgatcacct atgatgctgt
    22441 aaattctcac tcatctaaaa atgtggaacg tccatctttc aaaagttctg tccatattgc
    22501 aggagaatac aaggatggag tatttgatca agagagagat acttgggtcg gcaaagattc
    22561 aaacaatgaa atcactcagc tagaaggcta taaaggttat ctctataaca aaagtcattc
    22621 attagcatgg tcgctaggag gagatatgga ggtacacaat ttaacacttg gaacgagagc
    22681 gcaaaatgtt gggaaaaatg atcagaatgg agggatggct tactcagaaa cgattgtacg
    22741 tgatgcaatc tataaaaata agaatgaaaa agtattgtat aaagttgatc caatttataa
    22801 agggaatgaa ttagttcctc gtggttctca tgtcgaagct ttctcagtta acgataatgg
    22861 aaaaacggta aaccttaatg tttatgtatt taacaatcaa aaaggaatca acatcaacta
    22921 tcaaaatggt acttgggtaa gagattagaa attgagaagg tgaagaattg gaaacaataa
    22981 gcatcaatgg attagataaa tcagttgcaa atcgaataaa aaaaggaaat ggctactatc
    23041 ttctgaagaa gggtaaatat tcagttgcta gagagttaga aacaatgagt atcgtgtatg
    23101 tagctaagaa tcattttgac gtagaacagg ctatttttga tccagattct gaaataggga
    23161 aatgtgttga attatcagag aaaactgtta gtacaaaagg ggcgatggtc attgagagag
    23221 aaatgttaga cgaaatcaga gtaatcattc ctaaagatac gcagatgtgt gaggacagca
    23281 taagtgacga tgaaccaaaa tcagaaagaa aataaggaga tattcagatg agcgaaaaat
    23341 ccattgaatt aaaggaatat aagcgaagcg atggagaaaa atattatcct gtagccagtt
    23401 tttctaaatc agtatatctg gttggacgtg cattggtagt tggtgcaatt ggcatatata
    23461 ttttgcattg ggcaacaaag gttgtacaac tgcttgtaga taccttagta agtattcttg
    23521 tctcgttaaa tgatgttaac ggaacagatg atttaggatt aaaagagatg tttaaagagt
    23581 tactcaatgc gttttttccc aattatttaa gttattcata taatgtacaa agcttttttg
    23641 caaagctaca cttggtagac atctctattt tagtattaga agcaggaacg gtattctttg
    23701 ttggatattt ggttactttg atgttcaggc atcactatgg agaacaaaga ccattttttg
    23761 atgatatgga aagtagaagg ttgaaaagag aagctctaga agctatggat gtgaagtatg
    23821 atccaaacaa gattatagga gatgaaacga acaacaaaaa aagaaaacga aacaaaacgc
    23881 aattagaatc cgatgtgcta tcaacgctta aaaatatcaa aatttctgtt catacaagac
    23941 gtgatctaag tgtcaatgaa gatattcgag aatatatcgt aaaaatcccg caaccaaaag
    24001 ggaagaaagt tagagaaaca gttttacgac aaattcaaga tttagggtcg atcctgacaa
    24061 gagcaacaag cggacgagcc aaatttggtg aaaaagaaga tgaagtttca ggcgattatt
    24121 tcattttccg agcatcagta aatattgaaa gagaacgtaa ggaaagagta agtaccaaaa
    24181 acaaagaaac ggaaagagca acatcttcaa tcgcagtttc aaattcacaa ttttcttttc
    24241 caatcaatct ctttgccgat aattccaaaa atatccaaaa ccaaaccgaa aaagctagaa
    24301 aatatgccga aactcaaaag caatctttag aactttatct atcttctgct gacgtacctg
    24361 caagtctaac agatatgcac gttgggaata catctattga atatttgttt actttatctc
    24421 gtaccagaaa aattcaacta agctcaatga aagaagaaat tgaaacttac ttgcatatat
    24481 caggtgtaat cgtgcggatc aacgcagggc gtattcaaat tactttacca ttaggagaaa
    24541 atgatcgtat tccaattgat gtaccatcaa tgatcaatag cgtgttttag aagacatcac
    24601 aaatagaaac agaaaataca aactaccagt tcacaaacag aaaagtaccg agaaaagtga
    24661 ggaatagaaa tggctatgaa attattagag aaagtcaaaa agagagccga aaaagctgaa
    24721 tctattttgc tagataaaga acctaatata aagcttaatg gaaaattcag tgtcaatatg
    24781 ttgaacacaa aggctaaaga cccaacagat gctatttttg gaattaagcc tgatggcaca
    24841 attgctcatt tttgcatcag cgaaatgcca cacatgttgg ttgcaggaac gacaggatca
    24901 gggaaatcag tattactgaa tgaaattctt gttacagtaa tgtgtcactc aacgcctgat
    24961 gaattaaaaa taggaattgt agacccaaaa agggttgaat ttggaagata caaaaaattg
    25021 ccgttcatgt tggctgatcc aattacagat atggatgaag cttatgattt ttttgaatat
    25081 cttgtgatct tgatgcatga acgttacaaa ctgatggaaa aagcagggtt acaaaacatc
    25141 acgctatata acgaatatgc cgaaaaaaat ggattagata gatttcccta tgtgatctta
    25201 ttagttgatg aatattcaca gttggttgga acacataaag aagtagaagg tttgattgtt
    25261 caattaggac aaatggcaag ggcagcaggt attcatgtta tcttagcaac acaatctcct
    25321 cgatcaacag ttgtcactgg tattatcaaa gccaattttc cgtcacgtgt ttgccttatg
    25381 gtagcaagtg atttagaaag taggattgta ttagatgaag gcggtggaga aagtctaagt
    25441 ccaaagggag atatgcttat taagcttgtt gatggaagta tggttagagc gcagggagct
    25501 tacatcagta acaaagaaat tgaaacaatc ttcaatcacc ttagaaatac tatgccagaa
    25561 ccagaatatg ttgattaccg agcaatcgta gctgaaactc gtggtgaggg cggtgaagac
    25621 gaggattttg gtggaattgg taaagctcct gtaagaaaat ctgctaaaag aaatttgaac
    25681 acacctttag caaatccatc atctaaagag ccatcaattg aaaagaagag tgcgtttgct
    25741 attaagcttg aaaagaacaa agaagaaaaa gtcgagcagg gagctaaaac actagaaaaa
    25801 ctgcaagcag aaaaactaga aaaagtttta agggcattag gaaaagctga tcctgaaccg
    25861 caaacctcaa acaacacgaa taataaacag ccgattgcat ctaaaactaa acctaaagct
    25921 aatgatccaa aaggctcaga aaacgtagtg atcacaactg ataaatctga aaagaaacaa
    25981 ataaatggaa gtacttcacc tatcatgcaa aaaatgttaa aagcaaaaaa acaggtgcat
    26041 aaatgatcat cgtcagttct tttttaagat ggcttactcc atataaatat acagatgaag
    26101 tagttgtgaa acgagcgaag aaaagagata aaaaagcggt actaactcct aaacagatgc
    26161 ttgatcatgg cattttaggg gtgaaggttt tagaagtgaa taaaccgttt gaatcaaata
    26221 gtgcaagaga aaagttgctt ggcgctgaag ccgatgtcgc atatttttac catgacaaaa
    26281 gcaagcatca aatagctatt aaattcaacc cgaaatcggc atggcggtat caagcaagag
    26341 atgacttgaa acgtaggaaa cttaaaccaa tgctctatcc agaaggaaaa gaagcatttg
    26401 atagaaccca catcgttccc atcggttttc atggtacaga atcagataat cgcctattag
    26461 taggttggaa cagcaatcaa aatagaattc agatcaataa atttgagaag aaagtaagaa
    26521 aattcaacga tctaaaaaca atcttatggt ttgcagatat ttgtaaacag ccggacggaa
    26581 cagcaaagtg ggaagcggtg atatttgacg aaaatggtaa aaaagtaatg tctgaaacat
    26641 ggcatgataa agctaaattt gtttggcagt agcagtagag gtgaaaaaga ttgctagaac
    26701 tttttgaagt aaatagaaca ggctttaatc aaaaattaga agtgggagaa actatagaat
    26761 ataaagataa aaactatatt attatcggaa tctataacac gaggattaac agaataggtg
    26821 atccacgtat aaccagtgac ttggtttgtc aatctgttaa ctattcacct gatctgacaa
    26881 gcaggcataa gaaatatgtg ttattagaat atagacacaa gattactgat gaaattggag
    26941 taaataaaac acgaaatata tttaagatcg gaacagtgat tccttatagt aacaacgaag
    27001 aaaaaatatt ttatcagatt tacggaatag aaaagtttga atatgaacat gtcgatctgc
    27061 tagtgacgta ccaaatgcgt ttaatagaac cttggtcaca ggcagaaatt gataaagcag
    27121 taaaattgaa ccgattatca aaatttaacg tattaggaaa tgccttttaa aaatcgcaat
    27181 acacaaacag aaaaatacca aaaaattagc aatgaacgta ggtaagaaca tgacaataaa
    27241 taataataac cctaaaaaat ataatgctct tgtccaatgc tatatcgaaa acaaaattac
    27301 aatgcaagaa gtcgctgatg aattaggcaa atcaaaaagc ctagtatcac acgactttaa
    27361 gcacaatgtt tttccgaacc cgattgagct gaggaaaaaa tttcaaaaga gagtttatca
    27421 agaaataggg aagtggattg atgaagggaa tgcaattgac cctttaattg aaaaatatcc
    27481 ttttttaaaa agcaaaaata aaattcaaag gttaattcgt caaggcgata ttcaagctaa
    27541 agaacctgtt ttgacaacca atgatttggc taaaataaat cttcataaac agattattga
    27601 agactttgaa aaaaatcttt caacaactga aattttaaaa aaatacaata ttccgagagg
    27661 aaagctaaca tcgactcttt ttagagaata tggagaatcg gcagttacta aagcaaagga
    27721 agtagcgaat gagagaaaaa aacaaaaata gtagtcgata cctagtagcg actgtagttt
    27781 ttgtttgtgt attgattttc ctgtttgatt ttttgcagat tgggaaaagc ataattggaa
    27841 atacattgag tgcaaaggaa atggttgctt taggtggttt gtatgaagga catttgttcc
    27901 cgaatatatt catttctatc gtttctcata ttaacttgta ccatttaatt tctaatttat
    27961 tattcttagt agttcttgaa gaagcactga atgatcaata tgagtggtta acactattga
    28021 ttgttttaat catatcagga cttctaggaa gcttagttcc ttttctgtta caaagcaaca
    28081 tcgtaagtat ggggcttagt ggaggtattt ttggatggat tgctttattt gcgattgcat
    28141 acatccgctt tgatagtaag gaacatttat tactaagtgt ttttacatta ttgataatcg
    28201 gcattttact ggtatctact tttgaatcag gcgagacaaa cgtcttgtca catgtaatcg
    28261 gatttgtctg tgggatgatc ttgggattta ttctaaagaa cgtttcattg atcaagcaaa
    28321 agaattaaaa gggagtgcta aaaatgccaa aaaaaaaaga aaagaaaatt ccagtgtttt
    28381 tgtctgtctc agaaatgtta tcacttcaaa tactgttaag aaaaaagatt gaaacaatag
    28441 acgaagaagt tagaaataaa agggctatgt acgaacaacg tgaagcgcta agagaacgaa
    28501 tccactattt tgatgatata cacactaaaa tctatgaagc acgtacaaat aacgaagacg
    28561 aaagcaaatt aggattagct caacagcgaa tatttgaatg gctaaatacg aatactgagc
    28621 aagaaccgtt aagagcaatt agtaatcttt ttgaatttgg aatgaagcat tggtttaacg
    28681 cagtagaaaa ggatgctgac ttacaagaag cgtttgacac gctaagtaag gatagcaaat
    28741 ccatgcgaga tttattgatt acttatctaa atgagcatga gtttgaacaa acgaaaacca
    28801 aaaagaaaaa taaagaatag aaagttgtga tgaatcatgc tattaaccct gttaggttat
    28861 ttatgtttaa ttcttgcttt ttttggggca atcgctataa ttattggtca atttcaaaca
    28921 aataatccga ttatgcgtaa cataggtatt ggaattgtga ttgtaacctt tattgtgttt
    28981 aatgccacta agatttttgg aagtaacgat aaacaatttg gtatatcggg agttaataat
    29041 agttatgctc cagattcttt gggaagggta gtaatagatg gaacatttga aaatgtaacg
    29101 agttataaaa atgtttcgct gacagtgaag gaagatggca agaaagcatc agacgacgtt
    29161 ctcgctacta ttaagccgtc acaatcacaa tttgaagtgt tttatacagt caaagagaat
    29221 agccctgtac acaagctgac attggttttt aacgttgatg gagaagaata ctataaagat
    29281 gtaaaaataa ataaaacatc aacgaaaaag attatcgaat cagcgaaaaa agaagtagct
    29341 acagaagaaa gtacaactga atcaagttct actgtcgaag cggaactacc agataaaatt
    29401 gattttgatc ctgaaaaagt caaatcgttt gaatctgttt ctggttctat ggctgatcgg
    29461 tttaacactg aagtcttcaa agctatcaaa actgttaaat tggatactga aaagcaaatg
    29521 attaatgtta ccttattcaa ttcagttgaa tctatggatc aggataaaaa agttacggtt
    29581 gcaagagaac taggaaaaag agtaattggt ttgtatcgtg gtactgctaa accagattca
    29641 gacggtgcag atttgccagt tcatgtagtt tatgaagacg gtaaagtttt tggagattct
    29701 acagcaaacg aagcattaac gattaaattg gctaaatagc cagaaagtta taataaacaa
    29761 atggtgtcac accattaata tataggtgtg acaccttgga gatgatagct gatggtagta
    29821 aataaagaaa aagtaagaaa gtatgtgact ttaaaaatag cttctgttaa atatttagaa
    29881 aagcgagcta aagaagaaga aagagcaaga ggtaagaagg taacagtctc ggagctgtta
    29941 gaagaattga tcgagaatga tcaaacaata agacgtgcat ttactaatag gtctatcaga
    30001 taacaatata attaataaaa aagaaagagt gatttggtct tagactaaat cactcttttt
    30061 atataaagtt tctttacact tattccgaat aatgttaaaa ttctttatat gatatatagt
    30121 tacttgtcag agaaagagat aaccagcaat attggaggga taataagtgg aaaacaataa
    30181 tgatattaga cattttagca ttggggaaag agttacaaca gtaagtgaat tatcaaagga
    30241 ttatagagat aaagagggaa cgattatttc tcttaataag aatcgaagag gcgtttggca
    30301 atatgttgtt gatttaggtg atggcgttac agttgagtgt aaagatagtc aactgatcag
    30361 aaatgatggc agtcattcca ttgaaaaagc aattcttgta tacccaaaag tatcaagtaa
    30421 gatcatgaat gactggttgc ttggaaaatt tggtgagtac aataaaaaag aaatcgcaga
    30481 agtgatcggc aaatcttatc caacagtatt agggtatcta aaaaagataa cgaacaaaga
    30541 agcgaaagtt gaggatgttt taacagtaag agaacttcag tcattaatcg aatatcggaa
    30601 taaacaaata acaaatcctg agtaccttaa aaaagaagat ggtctattga aacaattgga
    30661 acaggtaaat aaaatccgga agatgcaaaa ggtagctcct ttaagcctag ataactttaa
    30721 aaaaatttat ttgaaagaga tcgaaaaaga ctaatgtgga gtgtcacttt accgtttcaa
    30781 cctgtatctg caccaagacc aagtgcaaga aggaatgttt ctgatgcaac taatgacgta
    30841 gcagagaaaa ggatttcaac ctattatgat aaaaagtatc tcgattattt gaaaaaaata
    30901 aaaagcttta tcgaagatca aggtttgtta gatgatgaat tttactcgat cgtaaatgat
    30961 ccgatgggtg caattatgga agtggacttt tactatcaga taccaaaaag ctataaaaaa
    31021 gtctataaca ttatgaaaac gacagcgcca gatttagata atttagtaaa aagtgctatg
    31081 gatggtattt tcaacatctc agctatacga gatagccata tcacaggctt aattgctttt
    31141 aaatataatg tggcgaacga aggaaaaaca gttgtgagga tcaaacgata ttcagaattt
    31201 gagtgggata cttctgaagg attgaacgga gatatatggg aagctacatt tgatttcaaa
    31261 ccagtagcaa cgccaagacc aaagtctaaa tttagaggaa acgggattat cacttactat
    31321 ccaaaagagt acagcgacta tttggaaaat atcaagaata cgcttaaaga gaaagatttg
    31381 gtaaatgatc aactgaaaaa ggtaatgaat gctcctatgg gggttatagc tcagatagat
    31441 tatttttacc aagtgagaaa agataagaaa aagcttgatt cactgatgcg tacttcacag
    31501 ccggatattg ataatttagt caaagggaca ttggacggaa tttttaacaa ggaaataggc
    31561 atcaaagata gcagggttgt agggttaatt gcttttaaat acaatacttt tgaaaagtcg
    31621 aaaacaaacg tgagactaca ggagatgggc tgatgaataa taatgaaaaa attcttcatg
    31681 tacttgatag ctttgaaaca attcaagaag aactaaagaa atatcgggat gttttggaac
    31741 aaagatatga ttttgtcaat cagcaaaaga gtaatcacat ggatttcatt ctcaatatga
    31801 atgacttgaa gaagaaatta ggtgaacgaa aagaacaaga aaagttgatc aaagcttact
    31861 ttaaactcgg tgaaaaagaa gttaaaaagg caatggaact gaatgaagat cgcagggttt
    31921 tagatcagct gttagaacaa ttattggtga tgtttcaaaa aggacgaata gatgaggatt
    31981 taattgaaga agggttgaga aaatatcctt caaactcagg tatcggaata gttctgaaag
    32041 cgattgatga agaagaaatt gaagctttca taccgccaga ggattttgaa tcggcaatgg
    32101 aatatataaa atattatagc caaggaatca ctgcttttag agagtttgat cctgaagatg
    32161 ttattcatga cttgaacaac ctaaaagaat ggtgtgaaag ttatgaagtc gatgattctg
    32221 gattggacta cttgatcagt attatggaaa tcgaagaaga gatgccggat aagcctgatc
    32281 caacagatat tttagaattg atccatgaag ctagaaatcc aattgcctat atttctagag
    32341 gatatactgt attggaatac tataagcctt atatatcagc gatgaaccat ttaagacgtg
    32401 tattaaggga aaaacgtgaa taccgctcag tgttgaatgc aatgaataga ttagaaaaag
    32461 ctgtttcaga gctagatgct tattatcgtg aacattacct gcaagcaggt ggtatgccaa
    32521 gaaatacgaa agcaaatatc agtaggtata taaaaaaagc tgagtaggcg gagttagccc
    32581 ataaaatggc aaaaggtaac ctgaaagggg cattttgaat gaaaagggat gtttttgaat
    32641 tttacgtatt agaacgtcta gaagacggca tgatggcaca agagataatt gatgaaattg
    32701 atgatatgat cagcagattt ggtcaattgg agttgaatga tggaaacaaa aattcggtag
    32761 tgctaaaaca attttggttg aataattttt cgtataagga ttttatcaaa gaaaaggcaa
    32821 atacattgta ctgactaaat tcacgaggat cagttatgag agcttattgc taagagcagt
    32881 taggctcttt ttttatttgt gtaaaattat tttctttgca atacacattt atacacaaaa
    32941 aatattgacg aatagtacac aaatgtagta ggatacattt gtaagcaagt tagattaaca
    33001 agttaaagga aggaagagca gttatgaaaa aagaagaact aatagatata ttttaaattg
    33061 ttgaacgagc aaataatatg ggaattatgt tttttgatcg tatttcttta aagatggatt
    33121 taagcattgc ccaccaagaa tttaatcttc gattaaaagc cttgttgaat tctgatgatg
    33181 ttaatttcgc tcatgatgtt gtaggtatac agaaccatat agatagagaa agcaaacgta
    33241 tgggtgatga attcttacca agatattcaa gtttatgatt tggcgaaaga ttcaagagta
    33301 tgtgtcatta ttaataggta ttcttgaatt ggaaaggagg aatgaattta agacaacaac
    33361 gattatgatc ttaaaacaat cgtttatgta attggcaatc ttacgaataa ttttcatgca
    33421 ataatgacaa taaaaaatga aaaggggtaa aatcaaatga aaaaaatttt agtaggaatt
    33481 ctattcacaa tggggctagt gttattatca ccttcagttt cagcagaaag ctctatttcc
    33541 atgtatcgta tgtacaatcc caatagcgga gagcatctat acactagaag cgttggagaa
    33601 agagacaatc tgaaacgtta tggatggcat tacgaaggtg tcgcatggca agctccaaca
    33661 agtggtactc ctgtttatcg tttgtataat cggtataacg gtgaacattt ttacaccgtt
    33721 gatgccggag aaagagataa gatttcacac acaggatgga cgtacgaagg tgttgcgttc
    33781 tattcctaca acggagcaaa tagagttcct gtaacacgtt tatataacaa atacgtgaat
    33841 tggcatcact acacgttaga tcaaaatgaa aagaatgtaa ttagtaaaca aggttggaag
    33901 attgaaggta caggctggta tgcaatgcca aacagcgtac caaatcctgc acctgctcca
    33961 agcactgaaa aagtgttgaa cgttcctgtt gtatggcaag gaaatacaat gttatgcgaa
    34021 ggagcttctt tattagaagg tttacactac aagggtgtag ccacaaatca aagcttaacc
    34081 agttttgtaa actatatgcc aaaagctaat gacaataatc cttatcatgg ttactcagga
    34141 gagtggcgtc ataacgttaa tggaacgtat caaggcatga tggctgaccc agtagttaaa
    34201 tgggcaaaaa acgttggcgg taatgccgtc aatatcactg gttcaggaac gaatggaatc
    34261 aagaatgaaa ttcgcaatgg taatccggtt gtaacttgga ttacttataa ctatgctacg
    34321 ccagagttca aacaaatgcc ttggggtcgt gcagtatgga atggacatgt agttctcgtg
    34381 gatggcttca gaaatggtgc ataccatgtt gttgaccctg tatttggcgc aaaatggatc
    34441 aatgcaagca cgtatgagag agcatttaat gtgactggaa tggcggttgc tgtacgttaa
    34501 ttgatagaat tatcaactaa atgaaataag agcttaatta ctgaaatggt aaataggctc
    34561 ttatttttta gagggaattt ttgtaatacg aatctataac aaaaaatatt gaatgcagta
    34621 cacaaaagtg gtatgttaaa atcgtagaga ttatgcgaag aggtgatttt gatgaaccaa
    34681 agaagaaaaa ggctagaaga aataagcgag cgactttccg agttaaagga tgagttagaa
    34741 gtaattcaag aagaagagga agagtacaaa gaaaatatac ctgaaaatct acagggatca
    34801 aaaagatatg aaaaagcaga agaatccgtt gaccaacttg aagaagctgt tgatggttta
    34861 tccgacatat taggattgat tgaagatata actagctaga gatgattaat gaagatttca
    34921 ggagtgagca aaaaatgatt gatatttcac tgatagagaa gctacttttc tcagataaat
    34981 acaccgctaa agaactggct acaagatggc aaatatccga atcagcaatt aaagcctatc
    35041 gtcaaaataa gggaagcgta ggatggcgtg attggaaagg gatcagcatt ggaaaagcag
    35101 aagacataat gaaacgtgaa ggacttcaat cctataccac ttctgaaaaa gacaaataac
    35161 ttaatgtttg aaaagataaa ttatctgtct taaagctatg tcaaattcaa atggtgaatg
    35221 tttgttaaat atattgcaaa aaaaggaaga gaaatattaa atgaaaagcg aaaatttgtc
    35281 ttatgagaat aataaacatc tagttaaaga aaataagaaa gtagaagatt ataagaacat
    35341 gtatatagaa atgaaaaaat caccactgtg tattcaatat aaaaatgata attggaagca
    35401 agtagtcgac tttgtaaaac ctcatagtat acgaagagta caaaggggaa gaaaaaaaag
    35461 tgaacttgta aatagcggaa gagcggtaat tagagttata acgaaagatt atccagttga
    35521 atatggagat tatattttaa ggttaagtta tcatgctttt gttgtgttga aagaagaaaa
    35581 tttcaacgag tttttcaata taaaaagtaa gtaatcatag aattttttct ggcgtgtata
    35641 aatttaatta aggagtaaca agaaatggaa tttgatgatc ttaaaaaaat cttactgaaa
    35701 aagaactcag cgagtttata gaagatttcg agaaatacaa agagacaaga aaaaaactgt
    35761 tgcagcaaag aaaaaaagat agagtaaaca aatctgattt gtattcgtca atacaaaaag
    35821 cattagaaaa tattactaaa gactaaaatt aaatttttat atttgacggt tgactatttt
    35881 gattaaggaa taacagctaa acagcgttca tgattgtgag gtgatgacat ggtagcaaaa
    35941 aagaatactc taaaagaagc ttctgaaaaa tatcaagata tgaaaagacg aggattaata
    36001 ccaaaggaca aaaaaggtat atggagagaa tttgttaagg aagtcagtag tgatcgaagt
    36061 ggcggtgact ttgttggacg atatacagca aaaggttata gcaggcgctt aaggatcact
    36121 gttgaatatg gaacgaatga atcagctaca gagctgaagc aaaggtactt agatacacga
    36181 agagaagctt caaaaaaata tgatgatttc aagagaagag gtctaattcc taagaagaaa
    36241 aagggaattt ggcgtgagtt tttcaaagaa gaagatagca atcggaataa tggagacttc
    36301 attgggcgat atacagcaaa aggctacact aagaatttag ggatcaaggt tgaatacgga
    36361 actggtgaaa gttcatggga attgaggaac cgctatttaa acatattgtt agagaaatac
    36421 aaagcagagt tattggagtt gttcgatgtt aatgatttta tcgagatggc tgaaaagtat
    36481 ggtcatgaag aaggttttat gatcaatgac attatgatta ttaataattt tacggattta
    36541 ttagcctttg cgaaaaaagc aagggattta agagaaacgc agatgtatct attcagaaat
    36601 aaaaacggaa gaaaccggag aactgaatcg agtgatatcg aagattacta cgatgccttt
    36661 acacacgatc taggagcctt tgtagacgag ataagtggcg taatggacaa atatagggtt
    36721 tagaataaaa aagccggaga tgagcagata aatgggaaac aatagcatat acacacctga
    36781 gatgcagaat aaaattaaaa gacttgtcgg attagggtgg atgacaagag gaaaattaga
    36841 ttcttttagt acaatcaaat atcttttgat ggacatttac cgtcagaaca agcaaacagg
    36901 aatacattat ttaggctatg ataagccgga agatgctcct ttctttgttc ctagttcagc
    36961 ctatagagcc agtacctccg gtagcatgta taacttggat ttgttcgagg aaggagcaaa
    37021 gatgatagag tatgatttca acgaagccta ttcgaagatc atgagaaatt atcctttgcc
    37081 gtcaaacact tatttgaaag caaaatataa tacagatcaa gctattttga gagcgataaa
    37141 tagaccaaaa gtaagaccgt atgaaaattt aagaacatat atgttctttt tggttaattt
    37201 tgaaggcaca gctaaagaag gaacttatgc aaacccaaaa tcacagtcga tgtttgcgga
    37261 ttatggcgaa aatcccaaaa cagctaatga acctatttgg ctatcagaga ttgagctaac
    37321 gcttatcctt gattactacg atattaaaga gatggaagtc ttagatagtc acacattttt
    37381 gacgaaaaaa ggattactca aagaatattt tgaacgaata gagccgttaa aaactgatcc
    37441 tgatacgttg aaactatata aaaatcttag aacaaaagta tttggagcta ttggacaaaa
    37501 aacgttaaga gaaggagaag taaacacttt tgataaatac agcatgtata atcgtgcgta
    37561 ctcctcgatg gtagcaggcg tttttagaga tcgcatggtt aggtatgaac agaaatacgt
    37621 tcacagcgaa tatgggctat taaaaatccg tactgatgga ttgtatttca gatcagaggt
    37681 gccagagttt gaagagctat ataaacttgg agtagtaaag aaaaaagttc atacaatatc
    37741 caaagaagaa gtcgaaaaag ccttagaatt caaaaataaa ggatttgaaa ataagggttc
    37801 ccttgggaag tctaaagatg aagttaaggg tgttgtgatt gatgatgatt tcgagtttga
    37861 ttttgactaa atactctttg gaaaagatgg taggaggtaa atatgaagaa gaaaattgtg
    37921 gaaggaaatg taggtgttat tattctaaaa gattcaactg actatgctaa atcaatggca
    37981 ctaaaagacg aaaaagttgc acgcatggat aaagatttag ttgaatttct gatggatgat
    38041 acagaaagat taaaagggga tcagtttttg agtttaatga aagacaaagg cttaaacaca
    38101 tatggtttat ttgattataa gaaattagct atagagtggg taaaagaagg agaaatattt
    38161 tttattgaag aatgggaagg tactgagaac ttagttcttc atcagtatct tttgaaagct
    38221 taatctaagt aaaacagttc tgacaagtta tgcttgtcag aatttcaaag gatgttttcc
    38281 ataaccatca tcattcaaaa ataaaggatt tgaaaataag ggttcctttg ggaaatctaa
    38341 tcatgtgaat attaatttaa caattaaacg tatttacgat ctgttatcac ttatataaag
    38401 agatatgtga taaataggac acaaaatttg ggaagtaggt aagtaacttt agtcagactt
    38461 ttagtctgac ttttttatat tttcagagat tcccctttac aaaaacgaac atacgtttgt
    38521 ataattgctt gtgaatgggg gaataaaaat gtttgattac agcagagaac cggtgaatga
    38581 ctatttttta atcgacatga agtctttcta tgctagcgtg gaatgtattg aaagaaattt
    38641 agacccaatg actacaccgt taattgtcat gagtagatcg gacaacactg gttcaggtct
    38701 gactctagct tcttcaccta ctgcaaagaa aatatatcat attactaatg ttagtagagc
    38761 tactgattta ccttatccat taccaaaaga ac
//
LOCUS       DACEJR010000019        38574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEJR010000019 DACEJR010000000
VERSION     DACEJR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.019"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            305..1348
                     /locus_tag="IW651_001005"
     CDS             305..1348
                     /locus_tag="IW651_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0813750.1"
                     /translation="MSEFKYYKSKEVYREKYYQMPKVFFTNEKYLNLSNDAKITYMLL
                     KARFDYSVQNNWVDSEDNIYFIYTIEELMRLLNCREGKISKIKKELEAAGLLKQKKGR
                     IKKANGKIETTPNLLYLGKPEVTSEDVFKINEEERLIPNPVNAKIANTEKDDDIKVIP
                     VNAKIANTEKASDTNSSPVNAKIADNILYSNNSLDTNRHNIDTDKDQLQDQLLLENFV
                     DIMTDSSFDTFIPTNVLKLIKTFSNSYSEAQQTVKTIHNAKHKAQESEKTIIVYEEIE
                     YYGVDADKGLYMTLLKAYQKQKTEKVDNLQNLIFIYVKNWFAEKAIPAKLAHEQNTET
                     NPLPAVNLENWLD"
     gene            2222..2614
                     /locus_tag="IW651_001006"
     CDS             2222..2614
                     /locus_tag="IW651_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgR"
                     /protein_id="HAQ0813751.1"
                     /translation="MELKPKNLKQITCEFVLFNRQHIKFSNRTIKRKITDFSGKEIIL
                     YAKKLSSEQELTCFFDQIKAIYFDGKIVACRGCSELPLIVSNHVFSKMKARQKLLRES
                     KVRYREMQKEEWEKRKSAARKKYKRISA"
     gene            3409..3691
                     /locus_tag="IW651_001007"
                     /pseudo
     CDS             3409..3691
                     /locus_tag="IW651_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3715..3912
                     /locus_tag="IW651_001008"
     CDS             3715..3912
                     /locus_tag="IW651_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813752.1"
                     /translation="MEWLKNEKELFHFLSDMKRKNQVANLDNYIKWIKDQSVQIKQDR
                     ESGNLNIKIAVTDNQNNILIY"
     gene            3923..4195
                     /locus_tag="IW651_001009"
     CDS             3923..4195
                     /locus_tag="IW651_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813753.1"
                     /translation="MIEEKKKVLYSNKPEFKKLVRQYAKKNIGRSITYDTFIKWLDKY
                     GYDLSQYDTCWQAVFKSLLQRNFQIDIAYRKTKECQLITVFQLNKS"
     gene            complement(4306..4725)
                     /locus_tag="IW651_001010"
     CDS             complement(4306..4725)
                     /locus_tag="IW651_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813754.1"
                     /translation="MKKSHIVTLATIFLLCVAAFFFFTNSPKNNKHITARTATTKSTI
                     KTETVYPSLSELKQLQGSDIEILGKTQITTVLFQNKNMAILTTENNKEIQLPLIVTDK
                     KNNLFEIPSLTYSKKNLKVGEVFGLAKKDNHYLAYKL"
     gene            complement(4771..5013)
                     /locus_tag="IW651_001011"
     CDS             complement(4771..5013)
                     /locus_tag="IW651_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813755.1"
                     /translation="MNITNIEELKEWIETNLVTKQEAIKITEQSPKGFQQSVRTNVIK
                     PFYESKEGKGPSVVRLYLKSDIETYAKQLKEKKQKP"
     gene            5164..6057
                     /locus_tag="IW651_001012"
     CDS             5164..6057
                     /locus_tag="IW651_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0813756.1"
                     /translation="MKLLKVLAVGVTVGGIALAINQEKVSAEEWTPRTVEQIKADIKG
                     NEYTIVWGDTLSGISQATNITVQKLADINKIANVDLIYAGNKLVFEGNVATVQNSAGE
                     TVAQTVIQDTDKVNPTQPVGQPAVSQDTTSNATNSADQSVAPTTPAGDSGANTPEQPV
                     QPSTPSDNGNQGGTVTPTPDQNQGGNNNQDNQGNQGGDTGNNGNQDNQGNNNGNNNNG
                     NNNGGNEGGDVTPPQPVEKWTVWYSASDEANAAHNIEKGTKVFDTEAEATAWIDQYAD
                     GLLAQGITSNSYGVSSFLVQE"
     gene            6309..9419
                     /locus_tag="IW651_001013"
     CDS             6309..9419
                     /locus_tag="IW651_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ0813757.1"
                     /translation="MILGALAMFLGFGLSSQSVNADNITMKPGEKGDIYYDASNVYTM
                     HGTKHGQPWSDATAAMAVSPNNGATDRIVFCIQPGVPFINTNNPDYEAVAVTDIPKDA
                     QIASVIWNTFYAFGTNFTNEDRIVTQAVIWELLPQYGIKVDSISGIPDFQAKKQKLYD
                     GIKEYKRLPEFNNKTIDLTFGETKKLTSSVDLNSFETQVSNSAKVNWNIAADGKSVDV
                     TPTDPNVTGGSISFKRSYMEGTPIALEKTGSQTVYLPSVKDPNNYSVNFRIQTVGDIT
                     IKKVDKETGEVVPNTQFKVEFSGKGAPSAQTVTTDKNGLATIKGINHGVHVKATEIAV
                     PAPYVLGSAIGDSDVVEGDVVAGQTVTLTQRNQKAKGQILIEKSGVETGKDMWNANYT
                     LAGNVFEIHEGNETGTVVRTITTDDKGHAETGKDLSLGTYVITEKTASNGFANTFKPV
                     TVKIEYANQTTAVIIKGTEGTNQEVTGSTLLTKEDAETGKETQGRATFDGAQYGLFHK
                     DGTPVKWSENFKPTTSKGNKLEGDEVKFELSDKEQQASVEHLALGDYYWQELVAPEGY
                     QIDNTKREFSITYKDQDTKVIETQSTSQENVIKFSLDGFKYVDSKSGDTKTGYNGIDF
                     TLTPIDPTKGEQRKVTTETDENGYDGYWAFNEVPYGDYKMSEVKAPEGYKTIKDLIIN
                     SKFDAEKREYTFTITEDGQKEPIKTLTVPEAKINEGSNVIHLSKLFLTNKVKEMPTIR
                     TKATVDGEKTFTPSKETPMQDVATITNVEKGVEYTNKIKLWRIVNDDYDNAKVVWEGS
                     HDFVAEGDKAEDVIKQMVDTSKDDNTVSYVFTEELFIKDGAKVAEHNDLTNKEQTIRP
                     VVPELPTIETLFVTKDGGKTFDPTKDQTLIDEVKATVPKEDIGQTFYYVTQFHKIDKN
                     GKVTVVGTDESEHKAGEMTYEFKAVYNYKANTLKDGEKLVATHIAYTDKEHKNEYAKH
                     FDLKNEKQTLTAKTPVTPATPTTPTTPQKSIPQTSGSLVNIGSLIILGIAAVAGAALY
                     VTTKKK"
     gene            9485..10522
                     /locus_tag="IW651_001014"
     CDS             9485..10522
                     /locus_tag="IW651_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0813758.1"
                     /translation="MFLGKGEINIVKKIIKALPLLWLLSSLILAICGFYGYTVLLRPE
                     NTIKQAQQITHPIQSEKEIKEIQQTTIGKATYNENQIQPVTPTDYAEAQLSYEALVNQ
                     WGIGSLYIPSSGIYSKILAGMSNDNLMVGLGTYYPNQLLGKGNYVLMAHNLVQGGGVL
                     RNLSQSSVGSTIYATDFSKIYEYEITTNKIVNQSESKLLDIPQEGDSPLMTIFRCEGG
                     LHTANRALIQARYVRSYSAENGSHDIKQALGLETTRNKTVNKQRLNDQQATSTNRTEA
                     AKESITPDKDTKKAKQTNKIEWRFTEKKAIYSNFQVFSILVFQLANAYPILIGLVFLV
                     GLSSCILLNRI"
     gene            10609..12135
                     /locus_tag="IW651_001015"
     CDS             10609..12135
                     /locus_tag="IW651_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0813759.1"
                     /translation="MKKKHISKVASAVLLSQMLLTTPLQVLADEQLSTPTSQSSTMNN
                     QSEESTSSSDEQTGTSTTQTTTDSTESSTDQSTTDDTTTSSTDTTSTTESSEKPTEDS
                     TTDSTSTTESSSESKPDETKPSEIKPSESKPEKPNTGSSTNESQTKPTTQPSNNQQAP
                     SVDPNNQATATNEGQQAKPAESESKPYHQTPTAPIQELVPSSKETAEGNIHFEKDESV
                     ESFVRKIGESARKVGQENDLYASIMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGK
                     GVSFATQEDLGDGTLYTTQATFRQYENYEDSLNDYAQLLKEGLTGNSQFYSGVWKTNA
                     KTYQEATKFLTGRYATDTKYDQKLNGLIETYHLTDYDKEVAGPQLSKEGYIVPVKNYT
                     ISSPFGTRGGEFHRGLDLAAAQGEPIYASKAGTVVKAEFHPSWGNYVAIEHEDGTTAL
                     YAHQQEYQVKVGDKVKQGQIIGYVGSTGNSTGSHLHFELCLDHSLSQSQLVDPETVLF
                     "
     gene            12314..12721
                     /locus_tag="IW651_001016"
     CDS             12314..12721
                     /locus_tag="IW651_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813760.1"
                     /translation="MTNRNEGITIYLTIGKEVKLNFDSENNLSSIRLSINKVAELFGN
                     KVVNEESIFYVSTVALVTKSNYKTFISVVGGKQVISGDAVYEEVMIDQERTSNKRGNK
                     NVYEQGYFEFFVSEEDKIPKEKLILPYNVKKRR"
     gene            12838..13899
                     /locus_tag="IW651_001017"
     CDS             12838..13899
                     /locus_tag="IW651_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ0813761.1"
                     /translation="MTFIDRKDNRVRQLFNMELIRAETKWYSFYQKNHRPPDYNFLQE
                     EITSFGVNRLPMFDRRYEGRVNDELSVDNFVHYHIQQLKLEKILSPVFFEKNDLRFME
                     RYAKNKAKAYFNERDQYIKGYESFRISRNKIMQRAGYEQLKYEFVSDNMVQGYFKEEV
                     AKMAANDERVAEFVFDEGKSLMRNLTQKMSRGGVSIINGSTKAVTNQIKKQRTTGKQR
                     YNQLKKHGALEHIDVSKTDLKPLANELKKYKVDFAVKRNKETGKIHVFFKAQDRDTLN
                     LALENVLQKFTNEKEQNREQQQEKPNKDIEKSSVDDKDKDKSLTKKLDKLEDKAQEIN
                     QQREQLKENTKNKTKGQER"
     gene            13974..15770
                     /locus_tag="IW651_001018"
     CDS             13974..15770
                     /locus_tag="IW651_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0813762.1"
                     /translation="MVYFSIPQNTDSSWINVVNEAFDKFLEPKTFTISPFTFSKIAMK
                     GGAIIAVIVLLIDLYIISGKKNLRMGEEHGSAKWGTSKDIAPYVDKKFKNNLLFTNTE
                     RITIETKLKKNKEYERNNNVVVVGGAGSGKTRFYVLPNLLQMHSSYVINDSKGGILSD
                     TAAIMQQNGYRIKVFNLNTMKNSDGYNPFHYIRRTEGDCEIDILKVVNNLMKNTSDPN
                     KKGGDSFWDDAQRSFLAAIFHLLILKGKPEEQNLGIVADLVRRARVKEDDEEYISSID
                     LVFQELEQEQPSCFAAKQYQNFKMAAGKTAKSILISLGVRLSPFDVPDIRKLTSTDTL
                     ELDTIGDRKTVLYIILPDTHTTMNFLASMIYQQLFDTLVYKADNVYNGSLPIPVRCLL
                     DEFANTGQIPDFENVIATTRSRGISVDVILQNLTQISKKLYKDSWETIIGNCDSFLYL
                     GGNEQSTHKYISTQLGKETIDVVTYNESRGTTGSFTKNSQKQGRNLLDPNEVREIKGG
                     KCIYMLRGTKPFLSDRFKLERHPLFKKLKETPSGYMYQSPLDQEEFIANNDPIDFELS
                     KNVDMKEFLKHTEIETYDFEDEEIETINLDEL"
     gene            15787..15987
                     /locus_tag="IW651_001019"
     CDS             15787..15987
                     /locus_tag="IW651_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813763.1"
                     /translation="MGTFNAIIDILSKYAIVGGGLYAVWGAVVLGGALKDHNGPQMQS
                     GIWQIVGGALIVAAAALFKTIV"
     gene            16016..16867
                     /locus_tag="IW651_001020"
     CDS             16016..16867
                     /locus_tag="IW651_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813764.1"
                     /translation="MMDGFLTWCVESILEVISFSSKTSHLTSNLENFSPQLYKYSTTI
                     LDSVSKPIAFSILALFFALELQRISNRVETNGGGQTGFEMIFKSLVRLGLCKLAMDQV
                     SLIMRAIMDITGYMTDKINHLGMGNKEVSDMLDAKKILAPILKEGLMFKVGVSTVLII
                     ALLITIGSIVIVQVVVNMRFIELYAFLAMSPIPIATFPNDEWSSIGKNFLKSFTATAL
                     QGTLIFMVMQFYPYLLSSAFSDITNSGSASETFLMTLIGIIGNSVLLIFAVMATGRWS
                     KSITNAM"
     gene            17132..17503
                     /locus_tag="IW651_001021"
     CDS             17132..17503
                     /locus_tag="IW651_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0813765.1"
                     /translation="MAIRVKVPKEIKDYQEKLMGGMSVRQLVFFVLAVLISVGVTAYC
                     VLVLHMSVDAAGYIVIPLLIPIVAFGWIRKEGMPLEKYGKIILNYYSKTGIRIYQTKE
                     RSNLNDFRKKRFQKVKETTKR"
     gene            17451..19901
                     /locus_tag="IW651_001022"
     CDS             17451..19901
                     /locus_tag="IW651_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0813766.1"
                     /translation="MILEKNDSKKSKKQPKDKQAKKVLKILAKQKKQRGKNQRRDAPP
                     LSSQRSLTYKAMYENGVCELKEGLYSRTFKFSDINYAIADQSEQELTFSSYCELLNSC
                     DSDTSLQISIINRSKDKKQFEKDLSYELVDDGLDVYRKEMNHMISNKIKYGNSSLQKE
                     KYITFNQRAKTLEQAIPALNRLQGDLEDQFSEMGCEVEELSGIERLRVVNDILRPNKK
                     LHFEYEDLLFSGLTTHSAIAPDSFDFKYRKDRFKMGDYYGQVLYLRNYPAELSDKLIT
                     NITDIPTNLQITLHIEPLDQQKGLDLVKTKLSFMEQQKIDEQKKAARSNYDPNLIPME
                     LRRSMDEAQELLDQMQGDNEKLFSFTFLVMAVDKDERKLDDTIEQILTAGRKNDCEFA
                     KLNYLQEEGLNSTLLWGNNFVPIHRTLTTASTAVFIPFTAQELMQPNGKYYGLNSVTK
                     NLVTLDRRKLKAPNGFILGTPGSGKSFATKKEMTAVLLSDPDAEVIVIDPEREYTPLA
                     EALNGEVIQISAGSKNHVNPMEINNDYADDDDPVILKTDFVLSLCEMLVGGNIGLSPT
                     QKTLIDRSCRFAYKKYFENGSGMPTLKDFSNILKDQPEDEARQLAVELELYTVGSLSV
                     FSHQTNVDTSKRFIVFDVKDLGKQLRSMGMLIILDQIWNRITSNRNAGRRTWVYIDEI
                     QLLFSNEYAENYFFELWSRARKWGAIPTGITQNVSTLLSSLNAERMLSNSDFVLMLNQ
                     SQRDRERLGELLHISSRQKKYITNAKEGAGLMYAGDSMIPLYDEFPKDTNLYKVMSTK
                     IEDVYKYQNEQREQGTTV"
     gene            19898..21745
                     /locus_tag="IW651_001023"
     CDS             19898..21745
                     /locus_tag="IW651_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0813767.1"
                     /translation="MNTDFRERIIRKQKTAKKFVVNRDNNQFKKKIVSTDYTKRAKKA
                     NHSGKIKSSRGTSYLIKKDSAKNVVKDVNSKAQKNGVEQGNATAELETSKTIGRGIKN
                     STKATKTVAKTTTKVTKKSKELIQTKASKKEVLTVVKKGSKKGLVNTSKAAKDTVINE
                     IQEFRGDSESQGINEAIQLKDFIDTGISGAKKTKKAANTAKKSSKALTKFGKSSYKYT
                     QRIAEIGIRRVQQVLSKEVAKALIAKLAIPLTLAVLILSIVTAVISTVNIFNFGAGDC
                     DTSANYDNITTKDIESNAKSIYSIIRKEIPESTPQGISGALGAMQFESQLNPNAVNPS
                     SGATGIAQWLGSRLTSLKEFAHKKGKKETDLGVQVEFLLSELNSSYYQSSKKILAMTD
                     VHEACKEWVMKFEGLSQDSSQWYLDQRNSYADHWYATLGTSDPIAEGTISNGADSELN
                     TLTCDLESDGGDIMEIAKSWLGWFHYDQIHPAPDLGTDLKNPNKNGRTDCSGFVWLVL
                     NKAGYKVPANMQWFTGSMASDARGSHQYLKQISETEAKAGDIVIVNQGAGAGSNGHTG
                     ILTEKWRGKDTKIIQEGGNGDSVNIEAFGTSFMSLLDGGDVVLARPIKK"
     gene            21765..22394
                     /locus_tag="IW651_001024"
     CDS             21765..22394
                     /locus_tag="IW651_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813768.1"
                     /translation="MLNRKSATITIFVLIVSVFSLSIGLNIKQELTVKTANSKIAALE
                     KKNDILKRETKDQKSRISKLEDENNKMIETQIVNKDGDVYKEFTNIATKYFKSRFNYD
                     PKTYKESKENVRDLISEELYKKFSENQLTYGDSNNVSSRLDNLEIYSGSLINQKTITG
                     LLIVDYESKLGETDWFKNSEIYTVQYDIHAKKLTKIQSLGSVLRGDMIE"
     gene            22387..22761
                     /locus_tag="IW651_001025"
     CDS             22387..22761
                     /locus_tag="IW651_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0813769.1"
                     /translation="MNKKFFSICVLIFTVSIIFLADHSYIKYIEAQNDPVYLDESNQE
                     KIVFYRNDCRNCEKIMPYLIRHNFLYHDLTFVNMNNKENKRYIKEYGLTSVPTIISLK
                     QRYVGTDISKIKTLVMSKGDDF"
     gene            22761..22994
                     /locus_tag="IW651_001026"
     CDS             22761..22994
                     /locus_tag="IW651_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2968 domain-containing protein"
                     /protein_id="HAQ0813770.1"
                     /translation="MARKSLTERIQQKKEEATKLEKRIQEDQYKLEKLTKQIRDLEAE
                     QVLAILDNHKLSVKDLNKILSKQPDNNNFINHN"
     gene            23067..23444
                     /locus_tag="IW651_001027"
     CDS             23067..23444
                     /locus_tag="IW651_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813771.1"
                     /translation="MLIAKRILASIVAIPCFLLSIIVIAVNIISIPILLVIQILKKIV
                     ILFITLVWIGFIGLFIYGLSSLGFSETLSMFTVPTIGILILTLILSIFTFISDKIPDF
                     LGIVSSFFLSIPKFLWGAEETEE"
     gene            23465..24532
                     /locus_tag="IW651_001028"
     CDS             23465..24532
                     /locus_tag="IW651_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813772.1"
                     /translation="MFLKKKYVRFIEQRDIELPETPVEIYRGIYLLEQENDRLTDKHT
                     KGSTLILLTDKKGNELNRRLIQLPIEQGIETILTNLLIEPTSNKKQSSSKSQMILNDI
                     EEPKIKNKKKTVNYQTVFFLFTTNLAKKIYMALIIVLFLFFTSFFVLNNHSSTSTDSQ
                     SYHELLKKKDYEKIIKQYPEKENELVEKLYSEKNSKELKELAEKSDSQIALFYWDFLN
                     EHWQAVTTMKNVTPNETIQAMRGYAYLKQGKLDEAELINKEIDNQTLKDQIYQFKKQE
                     AYKALHEKDIEKAEKINKEINDSELSEDIKVAKSMVNLLQKYEKDRSDSKLSEDERKE
                     AENNYKMWSENLKQLGGNDNA"
     gene            24525..26105
                     /locus_tag="IW651_001029"
     CDS             24525..26105
                     /locus_tag="IW651_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813773.1"
                     /translation="MHNKKKFLVVGLFSAVLLGGAVTYGMYSQQPHNETEKTEQTTKS
                     ESSKHKSKKSAWEESVKGKEDKKDKNKHSTKKADDILAVIADTPLVKQQPNQNKNNLI
                     DRLAVALDDQTKKEADRQEKLIIKDKKSVDEPVLVDLSYKPQFEDTKPSNKENGKTEN
                     NKDENSESDKGNDTKPDNSLPDPLPVPEPNPLPDPTPSPDPNPIPTPDPDPDPDPDPT
                     PDPEPSIDEQINQSKEELNDSRKQANELNKQLTQVKDSLDNLQSIGASTEKNVDKSAL
                     QWQKVADLVNEYNQLTNEIKKLIEEDGTVLPINYDLYKETYEKLNQKVTEVKTAQKEA
                     TNTTNEINKNVEQATDTLNNLEETKKEYQNKQKDVSEVDSNVNSAINHAQSNEQVASA
                     VQDEINQAAQSNEQLNETNKTVGEQLNQVDPIESETDIASAKEQTADINNQAEEQNNN
                     VDDMMNDFNNLPKPSDSEEPSVPENTDQNQDSTSQPQKIDNASVSQNKLENEQVSEKT
                     LPVTNQNSNQSEIEDIQK"
     gene            26124..27155
                     /locus_tag="IW651_001030"
     CDS             26124..27155
                     /locus_tag="IW651_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813774.1"
                     /translation="MSYKDSEAKKNNKTAELLSQAEEGIKEVLSSDKYKNFLRVMSKF
                     HNYSLNNTLLILMQNPNATHVAGYKTWKNSFNRQVNKGEKSIKIIGGRPYKRKVEEIK
                     NGQKEEKEVEGISFFPVSVFDVSQTSGEPLPQLMNELSGEVKNYPELYVALKQSTNFS
                     IEFADIQGGTKGYCSPLEKKIVLNNGMSQAQTIKTLIHEITHADLHAPEFDKNSSIKT
                     TKSTKEVEAESTAFVVCEHYGIDTKDYSFPYLAAWSSDKELKELKNSFEVILKQADNL
                     IKKIDKNLAELQKEVTKEKEEKQSTLSLSDQLEEFEDKAKSLNEQRDKEKLINKIRQT
                     KEQKDVSTL"
     gene            27237..27572
                     /locus_tag="IW651_001031"
     CDS             27237..27572
                     /locus_tag="IW651_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813775.1"
                     /translation="MKQYLDQWNIIEGALTVERINEIPDCLEKDHLFLIRKMLKDENL
                     EPNDFIVVEYPATGIYCCNHINNGQYFIIQEGKNKLVPYYTTCDMNEEGINNFPCTSI
                     KESMKLIEM"
     gene            27593..27862
                     /locus_tag="IW651_001032"
     CDS             27593..27862
                     /locus_tag="IW651_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813776.1"
                     /translation="MQTFTQHELEIISGHKQIAGSNANKLSVIKSEVGEMLSMLESDL
                     GKQYIEDDEQRHYTLKNYQSVYDKLEKMSEDEFKQIDLSGIEELE"
     gene            28098..28511
                     /locus_tag="IW651_001033"
     CDS             28098..28511
                     /locus_tag="IW651_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0813777.1"
                     /translation="MEQKLAKTPENILQIAKTYDNDERNIQKKFRVSEREKFLIDYKM
                     SQANTRNFSRYARIMCLSGLMITTDFSDLKNLTTELGRIGNNINQLAHRANISNSVSK
                     EDFLELKINYDQLTKLINEELLFRMRRARDNQRFI"
     gene            28511..30805
                     /locus_tag="IW651_001034"
     CDS             28511..30805
                     /locus_tag="IW651_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0813778.1"
                     /translation="MAVTKIHQIKTTLNKAIGYITNPEKTNNGMLVSSYNCQSQFAEV
                     EMQMTREYAREVKGDYRKVGGSEVLAHHLIQSFSPEDKVTPELAHELGRQLIDELTEG
                     KFEYVIATHIDQEHIHNHIIFNSVSFYDLKKFRSQPYRTANKIKEISNRICAEHDLSL
                     SPKKEKLKDSYKSYSNRRNNTSFRSEIRKRLTMCLNESTSWEEFEEKADHLGVNIDRK
                     GKHISYALLSEGQQRRTRGDKLDDLETFTESNISQKLSANQSYINQLKEAIEETFFSS
                     DNLADFSFLLEQEHGITFKLNKQDQFVFHFNDVNDFKWNESLLPDSYQIKNLKDYFRE
                     SYEFTNDSSLNSIEERYREKEKSVIVQEDTPIQLQEIQIEKATKEGLLVSIEHDDLNG
                     LVFVPNNYVDVNEETGEYTIWINDKFDYTLTDKQINEQTSFTVKGEGIIRGLEKMQGI
                     QPQEIKLSSKNILSMSNRGVSISIPELSIKRLFIPSEYVVIDEISKSCSILIGEKWNY
                     YAEPLPENDDNSNKKLPYQKFTGRSLISSLKETPELLDVFLKPKFDQLHYQSRKRYTN
                     QLVEALNTIREEKIVSASQLEQRLNEIVEQKNQAKEKIAEIDGKIKNYNNVAKLLVTY
                     EKYLPIMNEIERATMLQKIKLEQKYKEEVCQFKFAEKKLLASDNLRPDLTKEKIISVA
                     KNQEKQRKRLIEGYKHYENKLNRLIDVQEILQELDRNGLFDLSGYRLINREEKLTDKI
                     EKYEKEVEETQQKNSKDKSKEREI"
     gene            30944..31156
                     /locus_tag="IW651_001035"
     CDS             30944..31156
                     /locus_tag="IW651_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813779.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAIAKASDSVLAWLKFKKEQKK"
     gene            31322..32803
                     /locus_tag="IW651_001036"
     CDS             31322..32803
                     /locus_tag="IW651_001036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813780.1"
                     /translation="MTINIAYIFYFYIINFEKIYSLLEIVCEYITIEGLIATSSILVG
                     LLIPIAILLLENSSNLIDQSFKWDKLVIFSQVIKIRSIILGLTFITFPLILNDKTKNI
                     FPIILALYVIGLYFMFSLLKNAYYWIISKENEKESYRNSKRYQYLNSMTGNIANQITI
                     WDTIWEDAEQRIGLDELKLLKIFFKHCKVAEGQNKLNLLNTYLRDYEEDTFFKVTYSN
                     YEIIQDYIYHDLSIILNDSLSEEFGDYDLLVVIRNMFFKFTKQCYNASRIIIYKYNSK
                     FNSFFISSDSRILNRLFLNNFDIRFIETILNSRFTNDISLKENLPSCFNYGNSNLTAE
                     HQNIIVSMFSKYFDNYLNSADHYDVEKVLLLNELLKVVFNKVDLSLYITMENFRNYYS
                     KKIHNLLDLDDDSFMINFFKEYALMGDCVSTISENLFKDTEWTRNLLMSIYISRYPIF
                     TDEDRLNKLSQGISSTIESVELDTKGQKKLNYLKQKIDMLINL"
     gene            32957..33580
                     /locus_tag="IW651_001037"
     CDS             32957..33580
                     /locus_tag="IW651_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0813781.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQKLISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
     gene            33848..35095
                     /locus_tag="IW651_001038"
     CDS             33848..35095
                     /locus_tag="IW651_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAQ0813782.1"
                     /translation="MKNKLTLLICATILSTWAVPTQNAFAEETGNDTTQQTVTSNSSS
                     EEQTAIVNGEEVKLEAPVKEGQSEAEATQAPKEELQQDQMSRFARSAVRTIPMYRMYN
                     PNSGEHFYTQDTGERDNLRRIGWNYEGIGWQAPTSGDPVYRLYNRYNGEHFYTLNAKE
                     RDNITKQGWTYEGIGWYSYKGANGIPVSRLYNKRVNWHHYTLDEHEKNVISKQGWNYE
                     GVGWYAVGNGQPSNDDYKLLGVKNYNQYALGAPSGCEGASLLQGLQYKGKITNWSLTQ
                     FLNTIPKSPNGNPNNGFVGSPFVENSWTYSAIYPAPLATWGQKYGNVQNISGSSMNTL
                     LNEVKNGNPVVAWVTINFQPIRWGNWSFGVAANNNHAVTLDGYNKGSNQVHVSDPISG
                     SYWLNRTTFENIYNARKYAVVVR"
     gene            35367..36692
                     /locus_tag="IW651_001039"
     CDS             35367..36692
                     /locus_tag="IW651_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0813783.1"
                     /translation="MFDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMKLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIA
                     AVGVGDNPLLAKLALDNEAKNNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPSKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIIHASSKLEGARSITRSTLVGGHAGGVGGLKND"
     gene            36685..37035
                     /locus_tag="IW651_001040"
     CDS             36685..37035
                     /locus_tag="IW651_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813784.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDAENEQRANQNLAKEQMTTE
                     EITEVLNAAVIKNRSVCIQKEERNAEGYYPDDIIGKIFGSDELRIYVGKNKVHYDEIR
                     HIEFYQKHKWSCLE"
     gene            complement(37312..37674)
                     /locus_tag="IW651_001041"
     CDS             complement(37312..37674)
                     /locus_tag="IW651_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813785.1"
                     /translation="MTNEKNESIGLNAIKRKNKTQRPENIVPENSEALNNTTDSGKIL
                     EQSKKRLTTKDLPKSIRMPLDTHTAISTIATIEDKKIYETLNKIVEFYINNMNPANKK
                     IVKNSIKAVQNIYKDTDN"
     gene            complement(37688..38494)
                     /locus_tag="IW651_001042"
     CDS             complement(37688..38494)
                     /locus_tag="IW651_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0813786.1"
                     /translation="MTKVLTTGNFKGGVGKTTNAVLTAYTLSNKGYKVLVVDLDPQAN
                     ATELLFATMTNVYKIKPEFKETLFVSIQNNKISNSLISVKENLDLLPSFTDLEKYIDY
                     LAELYDDDYSKDTHFSNLLNEIKENYDFIIIDVPPQLNKFTNSALVASDYVIVILQTQ
                     ERSLKGAEKYIEHLIQLNNDYGTEIDILGLLPVLVQNGNDLDLDIIEDAESLFGKSNI
                     FNTKIKTMQRLKRYDRTGITDNPKDIHDKRVQQVYQSVSDEVIQRINLLG"
BASE COUNT        14692 a         5536 c         6950 g        11396 t
ORIGIN      
        1 ttatatagat ggatttaaag cgtttcatta tttcttaaaa taaatctttt ttatgaactt
       61 atatttttat aactttataa atttataatt ttatacgaag ctaatctgat taaaatgttt
      121 ttataaaaat attgagatta atggttttat tctgtatact aaatgtaaat acagaaaaga
      181 ccattgctga ttggattggc gtcctgcaat agtctttgtg taagtaatta aaagatttaa
      241 tttattatat tatagcatat tttaattact tactccacta catgaaaata aaaggagtaa
      301 tattatgtcg gaatttaaat actacaagtc aaaagaagtt tatcgtgaaa aatactatca
      361 aatgcctaaa gtatttttta caaacgagaa atatttaaat ttatcaaatg atgcgaaaat
      421 aacctatatg cttttgaaag cacgttttga ttactcagta caaaacaatt gggtagattc
      481 tgaggataat atttatttta tttacaccat tgaagagtta atgagattac taaactgtcg
      541 agaaggtaaa atttctaaaa taaaaaaaga attagaggca gcaggactgc tgaagcaaaa
      601 gaaaggacgt atcaaaaaag caaacggaaa aattgaaact acgccaaacc ttttgtatct
      661 aggtaaacct gaagtaactt cagaggacgt atttaaaata aacgaagaag aacgactaat
      721 cccaaatcct gtaaatgcga aaatcgcaaa tacagaaaaa gacgatgata tcaaggttat
      781 tcctgtaaat gcgaaaatcg caaatacaga aaaagccagt gacaccaata gttctcctgt
      841 aaatgcgaaa atcgcagaca atatattata ttctaataat tctttagaca ctaatagaca
      901 caatatagac actgacaaag atcagctaca agatcaatta ttgctagaga attttgttga
      961 tataatgact gattccagct ttgatacctt tataccaact aatgttttga aattgattaa
     1021 aactttttca aatagttaca gtgaagcaca acaaacagtc aaaactatac acaatgcaaa
     1081 gcataaagct caagaatcag aaaaaacaat tattgtttac gaagaaattg agtattatgg
     1141 agttgacgca gataaaggat tgtacatgac tttactgaag gcatatcaaa aacagaaaac
     1201 agaaaaagtt gataacctac aaaatctaat ttttatttac gtaaaaaact ggtttgctga
     1261 gaaagctatt cctgctaaat tggcacatga acaaaacaca gaaactaacc cattacctgc
     1321 tgtaaatcta gaaaattggt tagattagaa aaaaacgaac atatgtttgc aatataggcg
     1381 atgagtggta taattttatt gtgatatata ttgcatgatc atttaacaaa gaaaaaatta
     1441 gaataaatca tgttatttgg tttctaaatt ttcattaaaa ctttcatgag tgttctttat
     1501 tcgataacga tagtatctct aataaacaac gtgtaaagtg tgtaatgtaa gctttgttat
     1561 caatgatttt aaatgttaaa ataattccag aagcgcttca caactatgtg agtggaggtg
     1621 gtgtatatca aaatataatt gttttatgtt gttttacgat aattagtact tacacaaatt
     1681 tgtcaagtga aaaataaaaa agcttgtaat gaatttgaag atgtgctacc gtaaatttgt
     1741 agaaacgtaa attaaaaaaa ttattgatta gcaatccaga ctgctgatca ttgagaagcc
     1801 aagcaataaa tcaaccaaca aaaaagagca gaccgactag tgatctcgct gggtaagagc
     1861 gccaactctt acttcaggtc atgaatcgct agccgtcctc aattcaacgt atgtaccacg
     1921 ctgaagaggt aaactgccac ttttgttgca gatttaactg caacaggcat attctaacat
     1981 atatttctta agaatttaac tagtttcttt gtgttaccta aattcttttc aagaaaattt
     2041 gtatttctct ggctactttt tgaatagact tagtttgcaa ttttctagat atatttgttt
     2101 atactgtttg cagctgattt gtgacttaag agggcaatta cacttcatgt gtgattgccc
     2161 tctttttgtc gtgcttggct tcccaatgat cagcaactga agcagaaggg cggagaaaaa
     2221 tatggaatta aaacctaaaa atttaaaaca aattacttgc gaatttgtat tgtttaatcg
     2281 acaacacata aaattttcta atcgaacgat caagcgtaaa attactgatt tttcaggtaa
     2341 agaaattatt ttatacgcta aaaaattatc aagtgaacag gaattaactt gcttttttga
     2401 tcaaataaaa gctatctatt ttgacggcaa gatcgttgct tgtagaggct gttcggagtt
     2461 acctttaatt gtttcaaatc atgtgttttc aaaaatgaaa gcacgtcaga agctcttaag
     2521 agagtcgaaa gttcgatata gagaaatgca aaaagaggaa tgggaaaaaa gaaagagtgc
     2581 ggcacgcaaa aaatataaac gaatcagtgc ctagctactg ttttgactaa tgatacttga
     2641 tttttttgtt tgttagtttt ttctaacaaa caacctaata gcagggagtt attgctatcg
     2701 aaaaaacgga caagtaagca cacggacacg aaacagcgct aaatatttat ttaacgtttg
     2761 ctggcaagga gtcacatgac actaagcgat acacgtaaac ttactttgat gttttgggtg
     2821 ttccttgaca actgaaaaat tacaggactt tctaagttaa tcagcgtgtc taataggggc
     2881 ttaggaagga taaaccacct aaaaaagtac ggctgtgtta tcagatggta cgttattgat
     2941 gggtagggca cacacagaaa aggttatttg cgatgatcag ctatcacgaa aaacataaga
     3001 tgacgattaa aaccgtccgc tcgtttttct agggagcaaa aacacttttc gagagtttgg
     3061 ggttgctatc gctagaaacc ctgcgtaaaa aaactgtaac atttatgaga aaactaataa
     3121 acaaaagaat caagtatcta aaaataaggg gacacctaat ttaactggag ggtacaccta
     3181 tgagtactga gaaaaaagaa aaaagtatgc attttctgat gaaactatag aaaaattgga
     3241 ttggctagta aaagagcacc aaaaaagaag taaaaaacgt atttatcctt gtgatacact
     3301 ttctgaattg atcaataatg aatatgtgat aagaaagagt tttggcaatt cataagtaat
     3361 agtttacttc tttaattcct taacaagtat ataaaggagc tgagaaattt gaatcgtatt
     3421 cgatttggta aaagtaataa aaacgagttc tcatgacgaa gtgtattttg aatatgattt
     3481 agccactcat agaaattata atcctaaaga atctcatgaa gtatctttaa gaagagttca
     3541 atatctgact tgggatttat atccactttt aaaatatcca gaaagtgtaa ctagcaaact
     3601 tttttatatt gataaaagga ataacgaaaa agtagagaaa gcgtgggtta ctcttctaaa
     3661 actgcaaaaa attgctctag aagaaaaata aattaatttt tatgaggtgt tggtatggaa
     3721 tggttaaaaa atgaaaaaga gctttttcat tttctaagtg atatgaaaag gaagaaccaa
     3781 gtagcaaact tggataatta tatcaaatgg atcaaagatc agtcagtgca aataaaacaa
     3841 gatagagagt ctggtaactt aaatatcaaa attgcagtaa cggataatca aaacaacatt
     3901 ttaatttatt aaggagaata aaatgatcga agaaaaaaag aaagtattat attctaataa
     3961 acccgaattt aaaaaattgg ttaggcaata tgctaagaaa aatattggtc gtagcattac
     4021 ttacgatact tttattaaat ggttagataa atatggatat gatttgtcac aatatgatac
     4081 ttgctggcaa gcagtattta aatcactctt acaaagaaac tttcaaatcg acatagcgta
     4141 tagaaaaaca aaagaatgtc agcttattac agtatttcaa ttaaacaaaa gttaacgatt
     4201 atgatctcaa aatatcattt tatgattttg atacaataat cacaatatta tatagtaaaa
     4261 ttttttaaat gaggtgcaaa aatagagaga caaatgtctc cctatttata atttgtatgc
     4321 tagatagtga ttatcttttt tagctaaacc gaagacttca ccaactttta gatttttctt
     4381 tgagtaagtg aggcttggta tttcaaacaa gttgtttttc ttatcagtca caattaaggg
     4441 aagctgtatt tctttgttgt tctctgtagt tagaatagcc atgttcttat tttgaaataa
     4501 aacagtagta atttgagtct tccctagaat ttctatatct gaaccttgca attgttttag
     4561 ttcactcaaa gaaggataga cagtttctgt ttttattgta ctttttgtgg ttgctgtcct
     4621 cgctgtgata tgtttattgt ttttgggcga gttggtaaaa aagaaaaatg cagcaacgca
     4681 aagtaagaaa attgttgcta atgtaacgat gtgtgatttt ttcatagcat atctccatta
     4741 atatcaattt ataaaaagca gaaactgtaa ttatggtttc tgctttttct cttttaactg
     4801 tttagcatag gtttcaatat cgctttttag atagagacga actactgaag gaccttttcc
     4861 ttccttgctc tcataaaatg gttttattac atttgtgcga acagactgct gaaatccttt
     4921 aggagattgt tcagtaatct tgattgcttc ttgtttagta actaaattag tttcaatcca
     4981 ttcttttagt tcttctatat tagtgatgtt catattaatc ccacctttct gtggttttga
     5041 ttgtctacag aaatattata aatgaaaaaa agtaaaaaat ctaatataaa atggttgcaa
     5101 tactattccg ttactggtat agtattaata taataaaaat taaaaagaaa gaaggaataa
     5161 aacatgaaat tattgaaagt gttagctgtc ggtgtaacag taggaggaat tgcgttagca
     5221 atcaatcagg aaaaagtgag cgcagaagaa tggacaccac gtacagtaga acaaattaaa
     5281 gctgatatca aaggcaatga gtacacgatc gtttggggcg acactttaag tggtattagc
     5341 caagcaacaa acattactgt tcaaaaattg gctgatatca acaagattgc aaatgttgac
     5401 ttgatctatg cgggaaacaa attagtattt gaaggcaatg ttgcaactgt tcaaaatagc
     5461 gcaggtgaaa cagtagctca aacagttatt caagatacag acaaagttaa tccaactcaa
     5521 ccagtaggac aacctgcagt ttctcaagat acaacaagta atgcaactaa ctcagctgac
     5581 caatctgttg cacctactac accagctgga gatagcggag caaacactcc agaacaacca
     5641 gttcaacctt caacaccttc tgacaatggg aatcaaggtg gaacggtaac tccaactcct
     5701 gatcaaaatc aaggtggaaa taacaaccaa gataatcaag gaaatcaagg cggcgacact
     5761 ggaaataatg gaaaccaaga caatcaagga aacaataatg gaaataacaa caacggaaac
     5821 aacaatggcg gtaatgaagg tggcgatgta acaccaccgc aaccagttga aaaatggaca
     5881 gtttggtatt ctgcttcaga tgaagctaac gctgctcaca atattgaaaa aggaacaaaa
     5941 gtatttgata cagaagctga agcaacagca tggattgatc aatatgcaga tggcttgtta
     6001 gcacaaggta ttacttcaaa ttcttatggc gtatcttcat ttttagttca agaataataa
     6061 aatctgttta ccaagacact taagaaaatt cttaggtgtc ttttttttac ccttttttca
     6121 aattgctatt gaagggttta ttttcttaag gggggtgatt ccaatctaaa atttaatgtc
     6181 agtcggtgga gcgagccttt gggggtgctc tttttttatt gtacttatat atattgattg
     6241 aagagaggga gattatagtg agaataaaaa acaagagaaa taagtggaaa gttcccaagt
     6301 atctattgat gattttaggg gcattggcaa tgtttttagg atttggactt agttcgcaaa
     6361 gcgtaaatgc cgataatatt acgatgaagc cgggagaaaa aggcgatatc tattatgatg
     6421 caagtaatgt ttatactatg catggtacga agcatgggca accatggagc gacgcaacag
     6481 ctgcaatggc agtttctcct aacaatgggg ctacggatag aattgtattt tgtattcaac
     6541 ccggtgtgcc ttttattaat acaaataatc cagattatga agcagttgct gtaactgaca
     6601 ttcctaaaga cgcacaaatt gcttctgtca tttggaatac gttttatgct tttgggacta
     6661 actttaccaa tgaagatcga attgtcacac aagctgtcat ttgggaatta ttaccacaat
     6721 atggaatcaa agttgactct atcagtggta ttcctgattt tcaggctaaa aagcaaaaac
     6781 tttatgatgg aatcaaagaa tataaacgtt tgccagaatt taacaacaag acgattgatc
     6841 taacatttgg cgaaactaaa aaattaactt cttctgtaga tttaaattct tttgaaactc
     6901 aagtttctaa tagtgcaaaa gttaactgga acattgcagc tgatggtaaa tcagtcgatg
     6961 taactcctac tgatcctaat gtgacaggtg gaagtatttc ttttaaacgt tcatacatgg
     7021 aaggaacacc aatcgcatta gaaaaaacag gctcacaaac agtctactta ccaagtgtaa
     7081 aagaccctaa taactacagc gtgaatttcc gtattcaaac agttggagat attacaatta
     7141 aaaaagtaga taaagaaact ggcgaagttg ttcctaatac gcaatttaaa gtagagttta
     7201 gtggtaaagg agcgccttca gcacaaactg ttactactga taaaaatggt ttagcaacga
     7261 tcaaaggaat taaccatggt gtgcatgtca aagcaacaga aattgctgta ccagctcctt
     7321 atgttttagg atcagctatt ggcgattctg acgtagtaga aggcgatgtt gtcgcaggac
     7381 aaacagttac tttaacacaa cgtaaccaaa aagctaaagg acaaatcctg attgaaaaat
     7441 ctggtgtaga aacaggcaaa gatatgtgga atgctaacta tacattagca ggaaatgtat
     7501 ttgaaattca tgagggaaat gagacaggga cagttgttcg cactattaca acggacgaca
     7561 aaggtcatgc agaaacagga aaagatttgt cattaggtac ttatgtaatt actgaaaaaa
     7621 cagcttctaa tgggtttgct aacacattca aaccagtaac agtgaaaatc gaatatgcaa
     7681 accaaacaac agcagtaatt ataaaaggca ctgaaggaac gaaccaagaa gtaactggtt
     7741 caacattgtt aacgaaagaa gacgctgaaa caggcaaaga aacacaagga cgtgctactt
     7801 ttgatggggc acaatacgga ttattccaca aagacggtac tcctgtgaaa tggtctgaaa
     7861 acttcaaacc aacaacttct aaaggaaaca aattagaagg cgacgaagtg aaatttgaat
     7921 tatctgacaa agaacaacaa gcaagtgttg aacacttagc attaggtgac tactactggc
     7981 aagaacttgt tgctccagaa ggctaccaaa ttgataatac gaaacgtgaa ttttctatta
     8041 cttacaaaga tcaagataca aaagtaatcg aaactcaaag cacttcacaa gaaaatgtaa
     8101 tcaaatttag tcttgacggc tttaaatatg ttgattcaaa atcaggcgat actaagactg
     8161 gttacaatgg aattgacttc acgttaactc ctattgatcc aacaaaaggc gaacaacgta
     8221 aagtaacaac tgaaacagat gaaaacggct atgatggata ctgggcattc aatgaagtcc
     8281 cttatggcga ctacaaaatg tcagaagtaa aagctccaga aggctacaaa acaattaaag
     8341 atttaatcat taacagcaaa tttgatgctg aaaaacgtga atacacattt accattactg
     8401 aagacggaca aaaagaacca attaaaacat tgactgtacc agaagctaaa atcaatgaag
     8461 gttcaaatgt gattcattta agcaaattat tcttaactaa taaagtgaaa gaaatgccaa
     8521 ctatccgcac aaaagctact gttgacggag aaaaaacatt tactccttca aaagaaacac
     8581 caatgcaaga cgttgctaca attactaacg ttgaaaaagg tgtcgaatat acaaacaaaa
     8641 tcaaactttg gagaatcgtt aacgacgact atgacaatgc taaagttgtt tgggaaggtt
     8701 ctcatgattt tgtagctgaa ggcgataaag cagaagacgt tattaagcaa atggtagata
     8761 cttcaaaaga tgacaacact gtctcttacg tatttacgga agaattattc attaaagatg
     8821 gagcaaaagt tgcagaacac aacgacttaa cgaataaaga acaaacgatt cgtccagtcg
     8881 tgcctgaatt gccaactatt gaaacattat tcgtaacaaa agatggtggc aaaacatttg
     8941 atccaacgaa agatcaaaca ttaattgatg aagtaaaagc aactgtacct aaagaagata
     9001 tcggtcaaac attctactat gtaacacaat tccataaaat tgataaaaat ggaaaagtta
     9061 ctgttgtagg tacggacgaa tcagaacaca aagcaggcga aatgacttat gagttcaaag
     9121 cggtttataa ctacaaagcg aatactttga aagacggcga aaaattagtt gcgactcata
     9181 ttgcttatac agacaaagaa cacaaaaacg aatatgccaa acactttgat ttgaaaaatg
     9241 aaaaacaaac actaacggca aaaacaccag ttacgccagc aactccaaca actccaacaa
     9301 caccacaaaa atcaattcca caaacttctg gatcattggt taatattgga agcttaatta
     9361 ttttaggaat tgctgctgtt gcaggtgcag cattgtatgt tacaactaag aaaaagtaaa
     9421 taaaaccgaa aaataagaat gaaggggaag cactttgaaa aaggtgcttt tcctatttta
     9481 gttattgttt ttagggaaag gagagatcaa tatagtaaag aaaatcatta aagccttacc
     9541 gttattgtgg ctactaagta gcctgatttt agcaatctgt ggattttatg gttataccgt
     9601 tcttcttcgt cccgaaaata caatcaaaca agcacagcaa atcactcacc ctatacagtc
     9661 agagaaagag atcaaggaga tacaacaaac gacaataggg aaagcaacat acaatgaaaa
     9721 tcaaatccaa cctgttacac ctacagacta tgcggaagca cagctgagtt atgaggctct
     9781 tgttaaccag tggggaatcg gttctctcta tattccgtct tctggtatat actccaagat
     9841 tcttgcagga atgagcaatg acaatttaat ggttggcttg ggaacctatt atccaaacca
     9901 gttacttgga aaaggaaact atgttttgat ggcacataat ttagttcaag gtgggggagt
     9961 ccttcgtaat ttatcacaat ctagcgtagg atcaacaatt tatgcgactg attttagcaa
    10021 gatttatgaa tatgagatca ctacaaataa aattgttaac caatcagaga gtaagttatt
    10081 ggatatccca caagaaggtg attcgccttt aatgactatt tttagatgtg agggaggatt
    10141 acacacagct aatcgtgcat taattcaagc taggtatgtt cgatcttact cagctgaaaa
    10201 tggttcacat gatattaaac aagctttagg attagaaaca acgagaaata agacagtcaa
    10261 taaacaacgt ctaaatgacc aacaggcgac ttcaactaat agaactgaag cagcaaagga
    10321 aagtattact cccgataagg atactaaaaa ggcaaagcaa acaaataaaa tagaatggcg
    10381 cttcacagaa aagaaagcta tttattctaa cttccaagtg ttttctatcc tagtatttca
    10441 attagctaat gcttatccta ttctgattgg gttagttttt cttgttggtc tctcaagttg
    10501 tattctattg aacagaatat aatgagtgac aaaacttata tatattttta taaaaaattc
    10561 ataaaataaa gttatcttag atgtaacaca ggaaaggaaa gagaaaaagt gaaaaaaaag
    10621 catatttcaa aagtagcttc cgctgttctt ctttctcaaa tgctattgac aacaccgtta
    10681 caggtattag cagatgaaca gctttcaacg ccaaccagtc aatccagcac gatgaataat
    10741 cagtctgaag aatctacttc aagtagtgat gaacaaacag gaacgtctac tacacaaaca
    10801 acgactgatt ccacagaaag tagtacagat caaagtacaa ccgatgatac aacaactagc
    10861 tcgacagata cgacgtctac aacagaatcg tctgaaaagc ctacagaaga cagtacgaca
    10921 gattcaacaa gtacaacgga atccagtagt gagtctaaac cagatgaaac taaacctagc
    10981 gaaataaagc cgagtgaatc gaaaccagaa aagccgaaca ctggcagttc tacgaatgaa
    11041 tcacagacta agccaacaac tcaaccttca aacaatcagc aagcaccgtc tgttgatcca
    11101 aacaatcaag caacggcaac aaatgaaggg cagcaagcta aaccagcaga aagcgagtca
    11161 aaaccgtatc accaaacgcc aactgcgcct attcaagaat tagttccttc aagcaaagaa
    11221 acggctgaag gaaacattca ttttgaaaaa gatgagtccg tagaaagctt tgtccgaaaa
    11281 atcggcgagt ctgctcgcaa agtaggacag gagaacgact tatacgcttc tatcatgatt
    11341 gcacaagcca ttttggaatc agctagcggt caaagtcagt tagcgcaagc gccaaattat
    11401 aacttgttcg ggatcaaagg aacacataac ggaaaaggtg tttcttttgc tacacaagag
    11461 gatttaggtg acggcactct ttacaccaca caagctactt ttagacagta tgaaaactat
    11521 gaggatagct taaatgacta tgcacagctg cttaaagaag gtttgactgg taatagtcaa
    11581 ttttatagtg gtgtatggaa aacaaacgca aaaacttatc aagaagcaac caaattctta
    11641 actggcagat atgcaacaga tacaaagtat gatcaaaagc taaatggttt gatcgagacc
    11701 tatcatttaa cggactacga caaggaagtc gcaggaccac aattaagtaa agaaggatat
    11761 attgtaccag tgaaaaacta tacgatttct agtccgtttg gtactcgtgg gggagaattt
    11821 catagaggat tagatttggc agcagcacaa ggcgagccaa tctatgccag caaagcagga
    11881 acggtcgtta aagcggagtt tcacccttca tgggggaatt atgtagctat tgagcatgaa
    11941 gacggtacaa cggctttata tgctcaccaa caagaatatc aagtcaaagt tggcgacaaa
    12001 gtaaaacaag gtcagatcat tggctatgtt ggctcgacag gaaatagcac aggtagccat
    12061 ttacattttg aattatgttt agatcatagt ttaagtcaat cacaattggt tgatccagaa
    12121 acagtcttat tttaaaaata aggctgtttt ttttattcaa attttaagga ggtattgtct
    12181 atgttgctat ttataagtgg tttattttta ggcggattta ttggtgtggc gttaatgtca
    12241 ttagtagcaa ttgatcgtta tgaagatatg attgatgaaa gaagataatt aagaaagagg
    12301 gagtacaaat tttatgacga atagaaatga aggaatcact atctacttga ctattggtaa
    12361 agaagttaag ttaaattttg attcagagaa taacttatca agtattcgtc tatccattaa
    12421 taaagttgca gaacttttcg gaaataaagt agtcaatgaa gaaagtatat tttatgtttc
    12481 aacagtagcc ttagtgacaa aaagtaacta taagactttt atttcagtag taggtggaaa
    12541 gcaagttatt agtggagacg cagtttatga ggaagtaatg attgatcaag aaagaactag
    12601 caataaaaga ggaaacaaaa atgtatatga gcaaggatac tttgaatttt tcgtgtcaga
    12661 agaagacaaa attcctaaag agaaactaat tttgccttac aacgtaaaaa aacgtaggta
    12721 aaaagcttat gtgaggatat atctatgaat ataacagtaa aagaattagg cgaacctata
    12781 gaactcgaag attatttaga agaatataaa ttccaagtca tatttttatt aaaaaatatg
    12841 acttttattg atcgtaaaga taatcgggta agacaacttt ttaatatgga actgattcga
    12901 gcggaaacta agtggtattc tttttaccaa aaaaatcacc gtccacctga ttataacttt
    12961 ctacaagaag agattacgtc atttggtgtc aatcgtttgc cgatgtttga tagaagatat
    13021 gagggacgtg tgaatgacga attaagcgta gacaactttg ttcactatca tatacaacaa
    13081 ttaaagcttg aaaaaatact gtccccagtt ttttttgaaa aaaatgattt acgattcatg
    13141 gagagatacg caaaaaataa agcgaaagct tacttcaatg aacgtgatca atatataaaa
    13201 ggctatgaaa gttttagaat atctcgaaat aaaatcatgc aacgagcagg atatgaacaa
    13261 cttaaatatg aatttgtttc tgataatatg gttcaaggtt attttaaaga ggaggttgct
    13321 aaaatggcag caaatgacga acgagtggcg gaatttgttt ttgatgaagg taaaagctta
    13381 atgcgaaact taacacagaa aatgagcagg ggaggggtat caattattaa tggctcaaca
    13441 aaagcagtaa ctaatcaaat taaaaagcag agaactacag gaaaacagcg ctataatcaa
    13501 ctaaaaaagc atggagcgtt agaacatatt gatgtctcaa agactgattt aaagccactt
    13561 gcaaatgaac taaagaaata caaggtagat tttgcagtaa aaagaaacaa agagacgggt
    13621 aaaatacatg ttttctttaa agctcaagat agagatacac tcaatttagc attagaaaat
    13681 gtgctacaaa aatttactaa tgaaaaagaa cagaatagag aacaacagca ggagaaacct
    13741 aataaagata ttgaaaaatc ttctgtcgat gacaaggata aagataaatc gttaactaaa
    13801 aaacttgata aattagagga taaagcgcaa gaaataaatc aacaaagaga acaactaaaa
    13861 gagaacacga aaaataaaac taaggggcaa gaacgatgaa catcaccaaa aaagatctta
    13921 tttttggtgt cgcacttgct gcttttttct tttatttagg tactttattt tttatggttt
    13981 atttctcgat tcctcaaaat acagattctt catggataaa tgtagtaaat gaagcatttg
    14041 ataagttctt agaacctaag acatttacca tttctccatt tactttttca aaaattgcta
    14101 tgaaaggtgg ggcgataatt gcggttattg ttttactgat tgatttatat attataagtg
    14161 gcaagaaaaa tttaagaatg ggcgaagaac atgggagtgc caaatggggt acttctaaag
    14221 acattgctcc ttatgtagat aaaaaattta aaaataatct tttatttaca aatacagaaa
    14281 gaataacgat cgaaactaag ttaaaaaaga ataaggaata tgaacgtaat aataacgttg
    14341 tagttgtagg tggagctggt tcaggtaaaa ctagatttta tgtattgccc aatttattac
    14401 aaatgcactc aagctatgtt attaatgact ccaaaggtgg aatcttgagt gatacagctg
    14461 ccattatgca acaaaatggg tatcggataa aagtatttaa tctgaatacc atgaaaaatt
    14521 ctgatggtta taatcccttc cattatatta gaagaacaga aggagattgt gaaattgata
    14581 tattgaaagt agttaacaat ctaatgaaga atacttcaga ccctaacaaa aagggtggag
    14641 attcattttg ggacgatgca caaagatctt ttttggcagc tattttccat ttgttgattt
    14701 tgaaaggaaa acctgaagaa caaaatttag ggatagtagc agacttagta agacgcgcaa
    14761 gagtaaaaga agatgatgaa gaatatattt cttcgattga tttagtattt caagaattag
    14821 agcaagaaca gcctagttgt tttgcagcaa aacagtatca gaattttaag atggcagcag
    14881 gaaagacggc taagagtatt ttaattagtt taggtgttcg tttgtctcct tttgacgttc
    14941 cagatatcag aaaattaacg tcaacggaca ccttagaatt agatacaata ggagatcgta
    15001 aaactgtact ttatattatt ttgccagata ctcatactac tatgaacttt ctagcaagta
    15061 tgatttacca acaacttttt gatacgttag tttataaagc agataatgtt tataatggat
    15121 cgttaccaat tcctgtaagg tgtttgttag acgagtttgc taatactgga caaattccag
    15181 attttgaaaa tgttattgca acaacacgta gtagaggaat ttcagttgat gttatcttgc
    15241 aaaatttaac acagattagt aaaaagcttt ataaagacag ttgggaaaca atcataggaa
    15301 actgtgattc ttttttgtat ttaggaggaa acgagcaaag tactcataag tatatttcaa
    15361 ctcaattagg aaaagaaaca attgatgtag taacttataa cgaatcaagg ggaactacgg
    15421 gaagttttac taaaaactct caaaagcagg gaagaaacct acttgatcca aatgaagtgc
    15481 gtgagattaa aggcggaaaa tgtatttata tgttacgtgg cacaaagcct tttttatctg
    15541 atcgttttaa gttggaacgc caccctttat ttaaaaagtt aaaagagaca ccttcaggtt
    15601 atatgtatca atcaccgttg gatcaagaag aatttattgc aaataatgat ccaattgatt
    15661 ttgaattatc aaaaaatgtc gatatgaagg agtttttgaa acatacggaa atcgaaactt
    15721 atgattttga agacgaagaa attgaaacaa taaatttaga tgaattataa aaggagagaa
    15781 taattaatgg gaacatttaa cgctattata gatattttgt ccaaatacgc tattgtaggc
    15841 ggaggcttat atgcagtatg gggtgcagtt gttttaggtg gtgcattaaa agaccacaat
    15901 ggtccacaaa tgcaatcagg catttggcaa atcgttggtg gagctttaat tgtagcagca
    15961 gcggcgttat ttaagacaat tgtttagagg atagtcttta atgaaaaggg gatgaatgat
    16021 ggacggattt ttaacatggt gtgtcgaatc tattttagaa gtaatctcct ttagtagtaa
    16081 gacaagccac ttaactagca acttagaaaa ctttagtcct cagttataca aatattcaac
    16141 aactattctt gattctgtct caaagccaat agctttcagc atattggctt tgttttttgc
    16201 tttagaatta caaagaattt ctaatcgtgt ggagactaat ggtggaggtc aaactggttt
    16261 tgaaatgatt tttaaatcct tagtgcgact aggtctatgt aaactagcaa tggatcaagt
    16321 atctttaatt atgcgagcga ttatggatat tacaggatat atgactgata agattaatca
    16381 tttaggtatg ggaaacaagg aagtaagcga tatgctggac gcaaaaaaaa tattagctcc
    16441 cattttaaaa gaaggactaa tgtttaaagt aggtgtatcg actgttttaa ttatcgcatt
    16501 actaattact attggttcga tagtaatcgt acaggtagtt gtaaatatgc gttttattga
    16561 actatacgca ttcttagcta tgtctccaat tccaattgcg acatttccta atgatgaatg
    16621 gtcgagtata ggtaaaaact ttctaaaatc atttacagca acagcgttac agggaacttt
    16681 aatatttatg gttatgcagt tttatccata tctcttaagt tcggctttca gtgatataac
    16741 aaatagtggt tcagcaagtg aaacattctt aatgacactg attgggataa ttggtaatag
    16801 tgtattacta attttcgcag taatggcgac tggaagatgg tctaaatcca ttacaaatgc
    16861 aatgtaggaa ggtcttttca atgaaaaaat ctcgttgtaa cagcactcta attcttggag
    16921 tgctatttgt ttttttgcaa atactatcca tattattaac aacttatgat atttcaaatc
    16981 cattaacact aagtatactt tatgtatttt ttattgtttc tcttgttttt acactcataa
    17041 tgatcgtaat tattacaaaa gagatgttgc gagattttaa gaaattaata tctgatattt
    17101 tttcacagaa gaaagaaaag taggtgtttg agtggcaatt agagtaaaag taccaaaaga
    17161 aattaaggat tatcaagaaa aattaatggg aggaatgagc gttaggcagt tagtattttt
    17221 tgtattggca gtattaatct cagtaggagt aacagcatat tgtgtattgg tattgcatat
    17281 gtcggttgac gcagccggtt atatagttat cccactattg atacctattg tagcatttgg
    17341 ctggataaga aaagaaggta tgcctttaga aaaatatggc aagattattc taaattatta
    17401 ctcgaaaact ggaataagaa tatatcagac taaagaaagg agcaatctaa atgattttag
    17461 aaaaaaacga ttccaaaaag tcaaagaaac aaccaaaaga taagcaagca aaaaaagtat
    17521 taaaaatctt agcaaagcag aaaaaacagc gtggaaaaaa tcaacgtaga gacgctccac
    17581 ctttatctag ccaacgatca ttaacatata aagcaatgta tgagaatgga gtgtgtgagc
    17641 ttaaagaagg gctttattct cgtacattca aattttcaga tatcaattat gcaattgcag
    17701 atcaatctga gcaagaatta acttttagct cctattgtga gttactaaat agttgtgatt
    17761 ctgatacttc tttgcaaatt tcgattatta atagaagtaa agacaagaaa caatttgaaa
    17821 aagacttatc ttacgaatta gttgatgatg gactagatgt atatcgaaaa gaaatgaatc
    17881 atatgatatc taataagatt aaatatggga atagtagttt acaaaaagaa aaatatatta
    17941 cttttaatca aagagcaaaa actttagaac aagctatacc agctttaaat agacttcaag
    18001 gcgatttaga agatcaattt agcgaaatgg ggtgtgaagt agaagaactg tcaggaattg
    18061 agcgtctaag ggttgtcaat gatattctac gtcccaataa aaaacttcat tttgaatatg
    18121 aggatttact tttcagtggc ttaactacac attcagcaat tgcgccagat agttttgatt
    18181 ttaaatatcg taaggatcgt ttcaagatgg gggattatta cggtcaagtt ttgtatcttc
    18241 gtaattatcc tgctgaactt tcggataaat taattactaa tatcacagat atccctacta
    18301 atttacagat tactttgcat attgagcctt tagatcaaca aaagggatta gatttagtaa
    18361 aaacaaaatt atcatttatg gagcaacaaa agattgatga acagaaaaaa gcggctagaa
    18421 gtaattatga tcctaacttg ataccaatgg aattaagaag aagtatggac gaagcacaag
    18481 aattgttgga tcaaatgcaa ggagacaatg aaaagctctt ttcttttact tttttagtga
    18541 tggcggttga taaagatgaa agaaagcttg atgacacaat tgagcaaatt ttaactgcag
    18601 gtcggaaaaa tgactgcgag tttgcaaagt taaattatct tcaagaagaa ggtctaaact
    18661 ctacgctttt atggggaaac aattttgtcc ctatacatcg aacattaaca actgctagta
    18721 ctgctgtatt tattcctttt actgcacaag aacttatgca gccaaatggg aaatactacg
    18781 gtttaaactc agtaacaaaa aacttagtga cgttagatag gagaaaacta aaagctccta
    18841 atggttttat tttaggtact ccgggtagtg gaaaatcttt tgctactaaa aaagaaatga
    18901 cagctgtttt actgagtgat cccgatgctg aagtaatagt aattgatcct gaaagagaat
    18961 atacaccttt agcagaagct ttaaatggag aggttattca gatatccgca ggatcaaaaa
    19021 atcatgtgaa tccgatggaa attaataatg actatgcaga tgatgatgat ccagtcattt
    19081 taaagactga ctttgtgtta tctctttgcg aaatgttggt aggggggaat attggtctta
    19141 gtccgactca aaaaacttta attgatcgtt catgccgatt tgcctacaag aaatattttg
    19201 aaaatgggtc tggaatgcct actttaaaag atttttctaa tattcttaag gatcagcctg
    19261 aagatgaagc aagacaatta gcagtagagc ttgaacttta tactgtagga tcattatcag
    19321 tattttccca ccaaaccaat gttgatacga gcaaacgttt tatcgtattt gatgtaaaag
    19381 atttaggaaa acaattgcga tcgatgggca tgttgattat tctagatcaa atttggaatc
    19441 gtatcacaag taatcgaaat gcaggacgtc ggacatgggt atatattgac gaaattcagc
    19501 ttcttttttc aaatgaatat gcagagaatt acttttttga attatggtca agagcaagaa
    19561 aatggggagc aattcctaca ggtatcacac agaatgtatc tacgctccta tcttcattaa
    19621 atgcagaacg tatgctatca aatagtgatt ttgttctaat gttaaatcaa tcccaacgtg
    19681 atcgagaaag actaggcgaa ttgttacaca tttctagccg tcagaaaaaa tatattacga
    19741 atgccaaaga aggtgcagga ttgatgtatg cgggcgattc tatgattcca ctttatgatg
    19801 agtttccaaa agatactaac ttatataaag tgatgtcaac taagattgag gacgtgtata
    19861 agtatcaaaa tgagcaaaga gaacaaggta ctacagtatg aacacagatt ttcgagaacg
    19921 gattatccga aaacaaaaga cagcaaaaaa gtttgtagta aatcgagata ataatcagtt
    19981 caagaaaaaa attgtatcta ctgactatac aaaacgagct aagaaagcta atcatagtgg
    20041 aaaaataaaa agtagtcggg gcacttccta tttaataaaa aaagattcag caaaaaatgt
    20101 agtcaaagat gttaactcta aagcacaaaa aaatggtgtt gaacagggga atgcaactgc
    20161 tgaattagaa acgagtaaaa caataggacg tgggataaaa aatagcacta aggcaacaaa
    20221 aacagtggct aaaactacaa ctaaagttac taaaaaaagt aaagaattga ttcaaacaaa
    20281 ggcttcaaaa aaagaagttt taactgttgt taaaaaagga agtaaaaaag gtctcgtaaa
    20341 cacaagcaaa gctgcaaaag atacagtaat aaatgaaatc caagagttta gaggtgattc
    20401 agaatctcaa gggattaatg aagcgattca attaaaagat ttcattgata caggtatcag
    20461 tggtgctaaa aaaacaaaaa aagcagcaaa tactgctaaa aaaagtagta aagcactaac
    20521 aaaatttggt aaatcaagtt acaaatatac acaaagaatt gctgaaatag gcatacgtag
    20581 agttcaacaa gttttatcaa aagaagtagc aaaagcactc attgctaaat tagcgattcc
    20641 actaacttta gcagtgctta tattatcaat tgttacggct gtaatttcaa ctgttaacat
    20701 ttttaatttc ggagcaggcg actgtgatac tagtgccaac tatgacaata taactaccaa
    20761 agatatagag tcaaatgcta aaagtattta ctcaattatt cgtaaagaaa tacctgagtc
    20821 cacccctcaa gggatttctg gagcattagg agctatgcaa tttgaatctc aattgaatcc
    20881 aaatgcagtt aatccaagct caggtgcgac aggaattgcc cagtggttag gttctcgttt
    20941 aacctcttta aaagaatttg cacacaaaaa aggaaagaag gaaacagatt taggtgttca
    21001 agtagaattt ctgttatctg aattaaatag ttcatattat caatctagca aaaaaatatt
    21061 agcaatgaca gatgttcatg aagcatgcaa agagtgggtc atgaagtttg aaggattatc
    21121 tcaagactct agccaatggt atttggatca aagaaactct tatgctgatc attggtacgc
    21181 aactttaggg acgagtgatc caattgctga aggtactatt agtaatggtg cagatagcga
    21241 attaaatacg cttacttgtg atttagaatc tgatggtggc gatattatgg aaattgctaa
    21301 gagttggcta ggctggtttc actatgatca gatacaccct gctcctgatt taggaacaga
    21361 tttaaaaaat cctaataaaa atggaagaac tgattgctct ggttttgttt ggctagtttt
    21421 aaataaagct gggtataaag tacctgcaaa tatgcaatgg tttactggat caatggcaag
    21481 tgacgctaga ggatcacacc aatatttgaa gcaaatttca gagacagaag caaaagcagg
    21541 cgatattgtt attgttaatc aaggtgctgg agcaggtagt aatggacaca caggaatttt
    21601 gactgaaaag tggagaggta aagataccaa aatcattcaa gaaggtggaa atggcgatag
    21661 tgtaaatatt gaagcttttg gtacgtcttt tatgagctta ttagatggtg gcgatgttgt
    21721 attagcacgt cctataaaaa aataattaga ggggaggaag tactttgctc aacagaaaat
    21781 cagctactat aactatattt gtattgatcg tatcagtatt ttctttatcc attggattaa
    21841 atattaagca agagttaact gtaaaaactg caaattctaa aatagctgct ttagaaaaga
    21901 aaaacgatat tttaaaaaga gaaacgaaag accaaaagag ccgaattagt aaacttgaag
    21961 atgaaaataa taaaatgatt gaaacacaaa tagtcaataa agatggtgat gtctacaaag
    22021 aatttacaaa cattgctact aaatacttta aatcaagatt taattatgat cccaaaacat
    22081 acaaagaaag taaggaaaac gtaagagatt tgatttctga agagttgtat aagaaatttt
    22141 cggagaatca gctaacttat ggagattcta ataatgtgag ttctcgatta gataatttag
    22201 aaatatatag tggttcatta attaaccaaa aaactattac tggtcttttg attgttgact
    22261 atgaaagtaa attaggagaa actgactggt ttaaaaattc agaaatctat actgttcagt
    22321 atgatattca tgcgaagaaa ttgactaaaa tacaaagctt ggggtcagtt ttaaggggag
    22381 atatgattga ataaaaaatt tttttctatt tgtgtcttaa tttttactgt gtctatcata
    22441 tttttagcag atcattctta tatcaaatat atagaagcac aaaatgatcc agtctattta
    22501 gatgaatcaa atcaagagaa aatagttttc tacagaaacg attgtagaaa ctgtgaaaag
    22561 atcatgcctt acctgatacg tcacaatttt ttataccatg acttaacttt tgttaacatg
    22621 aataacaaag aaaataaacg ttatattaag gagtatggtt tgacttccgt tcctacaatc
    22681 atttccctta agcagcgtta tgtaggtaca gatataagta aaataaaaac attagttatg
    22741 tcaaaaggag atgatttctg atggcaagaa agagcttaac agaacgtatt cagcagaaga
    22801 aagaagaagc aacaaagctt gaaaagagga ttcaagaaga tcaatataag ctagaaaaac
    22861 taacaaagca aattagagat ttagaagcag aacaggtttt agcgattctt gataaccata
    22921 agcttagtgt gaaagatttg aataaaatac taagtaaaca gcctgataac aataatttta
    22981 tcaaccataa ttaattaaat atatatcttt ttaattgaga taataagcga tataataaga
    23041 taaacaagta ataagagggt gaaagtatgt taatagctaa aagaatatta gcaagtatag
    23101 tagcaattcc ttgcttttta ctttccataa ttgttattgc cgttaacata atttccatac
    23161 ctattttgct tgttatacag attcttaaaa aaattgtgat cctttttatt acattagtat
    23221 ggattggttt tataggtctg tttatttatg gattaagttc tttaggcttc tctgaaacgc
    23281 ttagtatgtt tacagtgcct actataggta tattaatcct tacattaata ctatctattt
    23341 ttacttttat ctcagataaa attcctgatt ttcttggaat tgttagttca tttttccttt
    23401 ctatcccaaa atttctatgg ggggctgaag aaactgaaga ataaaacatt caagggggaa
    23461 aaatatgttt ttaaagaaaa aatatgttcg gtttatagaa caaagagata ttgaattgcc
    23521 agaaacccct gtagaaattt acaggggtat ttatttactt gaacaggaaa acgatcggct
    23581 aacagacaag catactaaag gttctacatt aatattatta accgataaaa agggaaatga
    23641 actaaataga cgactaatcc aattaccaat agaacaaggt atagagacta ttttgactaa
    23701 ccttttaatt gaacctacct caaataaaaa gcaaagttct tctaagtcac aaatgatatt
    23761 gaatgatata gaagaaccaa aaataaagaa taaaaagaag accgtaaatt atcagacggt
    23821 cttcttttta tttactacta atttagcaaa aaaaatctac atggctttga taatagttct
    23881 gttcctattt ttcacaagtt tttttgtatt aaataatcat tcatcaactt ctactgattc
    23941 tcaaagctat catgagctgt taaagaagaa agattatgaa aaaataataa aacaatatcc
    24001 tgaaaaagaa aacgaattag tagaaaaact gtatagcgaa aaaaattcta aggaattaaa
    24061 agaattggca gagaaatctg attcacagat agctcttttt tattgggatt tcttgaatga
    24121 gcattggcaa gcagtaacta cgatgaaaaa tgttacacca aacgaaacaa tacaagctat
    24181 gcgtggttat gcatacttaa aacaaggaaa actagacgaa gcagaactaa ttaacaaaga
    24241 aattgataat caaacattaa aagatcaaat ttatcaattt aaaaagcaag aagcatataa
    24301 agctctgcat gaaaaagata tcgagaaggc agagaaaata aataaagaaa taaatgattc
    24361 tgaactatcc gaagatatta aagtagctaa aagtatggtt aatctacttc aaaaatatga
    24421 aaaggacaga tcggattcaa aattatcaga agatgaaaga aaagaagctg agaacaatta
    24481 caaaatgtgg tcagaaaact taaaacaact aggaggaaac gataatgcat aacaaaaaga
    24541 aatttttagt agtgggacta ttttcagctg ttttattagg gggtgcagtt acttatggca
    24601 tgtattctca acaaccacac aatgagactg aaaagacaga acaaacaact aaatcagagt
    24661 ccagcaaaca caaaagtaag aaatcagcat gggaagaaag tgtgaaaggc aaagaagaca
    24721 aaaaggacaa aaacaaacat tctacaaaga aagctgatga tatattggct gttattgctg
    24781 atacaccgtt ggttaaacaa caacctaatc aaaacaaaaa taatctaatt gatagattag
    24841 cagtagcttt agatgatcag actaaaaaag aggcagacag acaagaaaaa ttaattatta
    24901 aagataaaaa atctgtagat gagccagtac tagttgattt gagttataaa cctcaatttg
    24961 aggatactaa accttcaaat aaagagaacg gtaaaacaga aaataataaa gatgaaaata
    25021 gcgaatcaga taaaggaaat gatactaaac ctgataattc attacctgat ccattgcctg
    25081 taccagagcc aaatccattg cctgatccaa cacctagtcc agacccaaat ccaataccaa
    25141 caccagatcc agaccctgat ccagatcctg atccaacacc agatccagaa ccttcaattg
    25201 atgaacaaat taatcaatct aaggaagaac tcaatgattc taggaaacag gctaatgaat
    25261 taaataaaca actaactcaa gtaaaagata gcttggataa cttacaaagt attggagcaa
    25321 gtactgaaaa gaacgtggat aaatcagcat tacaatggca aaaagttgct gacttagtca
    25381 atgaatataa tcaacttaca aatgagatca agaaattaat tgaagaagac ggaacagtgt
    25441 tgccaatcaa ttatgatctt tataaagaaa cgtatgaaaa actaaaccaa aaagtcactg
    25501 aagtaaaaac tgcacaaaaa gaagcaacta atacgacaaa tgaaataaat aaaaatgtag
    25561 aacaagcaac tgatacgcta aataatttag aagaaactaa aaaagaatat caaaataaac
    25621 agaaagatgt ttctgaggtg gattctaacg tcaattcggc tattaaccat gcacaatcta
    25681 acgaacaagt tgcttcagct gttcaagatg agataaatca ggctgcacaa tctaatgagc
    25741 aactaaatga aactaataag actgttggag aacagttgaa tcaagttgat ccaattgaaa
    25801 gcgaaactga tattgctagc gctaaggagc aaactgctga tatcaataat caagctgaag
    25861 aacaaaataa taatgttgac gatatgatga atgattttaa caatttgcca aaaccgagtg
    25921 atagtgaaga acctagcgtt ccagaaaata cagatcaaaa tcaagattca acaagccaac
    25981 cacaaaaaat agataatgct agtgtttctc aaaacaaatt agaaaatgaa caggttagcg
    26041 aaaaaacatt accagttact aatcaaaata gtaaccaatc cgaaattgaa gatattcaaa
    26101 aataaaagtg aggaatgaaa taaatgtctt ataaagatag tgaagcaaaa aaaaataata
    26161 aaaccgctga attactttca caggcagaag aaggtattaa agaagtatta tcttcagaca
    26221 aatacaaaaa ttttcttcgt gttatgtcta agtttcataa ttacagctta aataataccc
    26281 ttttaattct tatgcaaaat cctaatgcta ctcatgttgc tggttacaaa acatggaaaa
    26341 actcttttaa tagacaagta aataaaggag aaaaatccat taaaataatt ggcggtcgtc
    26401 cctataagag gaaagtagaa gaaataaaga atgggcaaaa agaagaaaaa gaagttgaag
    26461 gtatttcatt tttccctgtt tcagtttttg atgtttctca aacttcagga gaacctttac
    26521 cgcaactaat gaatgaatta agtggagaag taaaaaatta tccagagctt tatgttgcct
    26581 tgaagcaaag taccaatttt tctatagaat ttgcagatat acaaggtggc acaaaaggat
    26641 attgttctcc attagaaaag aaaattgtat taaataatgg tatgagtcaa gcgcaaacaa
    26701 taaaaactct gattcatgag atcactcatg ctgatttaca tgcaccagaa tttgataaaa
    26761 attcgtcaat aaaaactaca aaatctacaa aagaagttga agctgaaagt acggcttttg
    26821 ttgtttgtga acattatgga atagatacaa aagactatag ctttccatat ttagcagctt
    26881 ggtctagtga taaagagtta aaggaattga aaaatagctt tgaagtcatt ttaaagcaag
    26941 cagataattt aattaaaaaa atagacaaga atttagcaga attacaaaaa gaagtgacaa
    27001 aagaaaaaga agaaaaacag agtactttgt cattatctga tcaattagaa gaatttgaag
    27061 ataaagcaaa atctttaaat gaacaacgtg acaaagaaaa attaattaat aaaattcgcc
    27121 aaacaaaaga gcaaaaagac gtttctacac tatagtttaa agtagatgaa acgatctatt
    27181 ttgctttttt aatttatcag aaaaagtaac ctttataata aatgagggat ataaaaatga
    27241 aacaatattt agaccaatgg aatataattg aaggagcatt aactgtagaa agaattaatg
    27301 aaatacctga ttgtcttgaa aaggatcatt tatttctaat tagaaaaatg ctaaaagatg
    27361 agaatcttga acccaatgat tttattgttg ttgaatatcc agctactgga atatattgct
    27421 gtaatcatat taacaacggt caatatttta ttattcaaga aggaaaaaat aaattagttc
    27481 catactatac tacttgtgat atgaatgaag agggaattaa taattttcct tgtacgagta
    27541 tcaaagaatc catgaaatta atagaaatgt aaaagagagg gacatataaa tcatgcaaac
    27601 atttactcaa cacgaattag aaattattag cggacacaaa cagattgcag gttcaaatgc
    27661 aaataaatta agcgtcataa aaagcgaagt tggagaaatg ctttcaatgt tagaaagtga
    27721 tttaggaaaa caatatattg aagatgatga acaacgacat tacacgttaa agaattatca
    27781 gtctgtttat gataaactcg aaaaaatgag tgaagatgaa tttaaacaaa ttgatttaag
    27841 cggtattgaa gaattggaat agaaacgggg attggggcag cgccccaaaa tgcaaaaaga
    27901 agaagtcgcg tgagcggctt tttctttttt ttccgcaagt tggtaccaac ttgcaaatct
    27961 tgctgtaaga ccgcccccct gaaatcttcg atttctaatc ggagggcggt cacacagtgg
    28021 aaaatttcgt ttttaaacaa agataaaaca ctaaactagt gaaaaaaaac aaaaaatatt
    28081 ggtaaggagg gggaaatatg gagcaaaaac tagctaaaac tcctgaaaat attctacaaa
    28141 tcgctaaaac ttacgacaac gatgaaagaa atattcaaaa aaaatttaga gtatctgaaa
    28201 gagaaaaatt cttaatagac tacaaaatga gtcaagcaaa tacaagaaat ttttcccgat
    28261 acgctcgtat tatgtgttta agtggtttaa tgattacaac agatttttct gatttaaaaa
    28321 atttaactac agaattagga cgaattggaa ataatatcaa tcaattagct cacagagcta
    28381 atatttcaaa cagtgtttca aaagaagact ttttagaatt aaaaataaat tatgatcaat
    28441 tgactaaatt aattaacgaa gaattacttt ttagaatgcg acgtgctaga gataatcaga
    28501 ggtttattta atggcagtga caaaaataca ccaaataaaa actactttaa ataaagcaat
    28561 tggctatatt acaaatccag aaaaaacaaa taatggaatg ctggttagta gctataattg
    28621 tcaaagtcaa tttgcagaag ttgaaatgca aatgactagg gaatatgcac gtgaagtaaa
    28681 aggagattat cgaaaagtcg gtgggagtga ggttttagcg caccacttaa tccaaagttt
    28741 ttcgcctgaa gacaaagtga caccagagct tgctcatgaa ttaggacgac aattgattga
    28801 tgaattgact gaaggaaagt ttgaatatgt gattgccact cacatagatc aagaacatat
    28861 tcataatcac attattttta acagtgtttc tttttatgat ctgaaaaaat tcagaagtca
    28921 accttatcga acagcaaata aaattaaaga aattagtaat cgtatttgtg ctgaacatga
    28981 tttatcttta tcaccgaaaa aagaaaaatt aaaagattcc tacaaatctt attctaacag
    29041 aagaaataac acgagctttc gttctgaaat tcgtaaacga ttgaccatgt gcttaaatga
    29101 atcaacttca tgggaggaat ttgaagaaaa agcagatcat ttaggggtaa atattgatcg
    29161 aaaagggaaa catatttcgt atgctttatt atccgaagga caacaaagac ggactagagg
    29221 ggacaagtta gatgacttag aaacatttac agaaagtaat atttcccaaa aactttctgc
    29281 taatcaatcc tacattaatc agttgaaaga agcgattgaa gagacattct tttctagtga
    29341 caacttggca gattttagtt ttcttttgga acaagagcat ggaattacct ttaagttaaa
    29401 taaacaagat caatttgttt tccatttcaa tgatgtaaat gattttaaat ggaatgaaag
    29461 cttgctgcca gatagttatc agattaaaaa tctaaaagat tattttagag aatcctatga
    29521 gtttacgaat gactcttcct taaattcaat tgaagaaaga tatcgtgaaa aagaaaaaag
    29581 tgtgattgtt caagaagata cgcctattca attacaagaa attcagatag agaaagcgac
    29641 caaagaagga ttgttagtgt ctatcgaaca tgacgactta aatggtttag tgtttgtccc
    29701 aaataattat gttgatgtaa atgaagaaac tggagaatat acaatttgga tcaatgacaa
    29761 atttgattat acacttactg ataaacagat aaacgaacaa actagtttta ctgtaaaagg
    29821 ggaaggaatc attcgtggac tggaaaaaat gcaagggatt cagccacaag agataaaact
    29881 ttctagcaaa aatattttat ctatgagtaa tcgtggtgta tctatttcga taccagaact
    29941 gagtatcaag cggctcttta ttcctagtga gtatgttgta atcgatgaaa ttagtaaatc
    30001 ttgttctatc ttaattgggg aaaagtggaa ttactatgca gaaccactcc ctgagaacga
    30061 tgataattca aataaaaaat taccttatca aaaatttact ggacgttcat taatcagtag
    30121 tttaaaagaa actcctgaat tacttgatgt gtttctaaaa cctaaatttg atcagctaca
    30181 ttatcaatca aggaaacgat atactaatca attagtcgaa gctttaaata cgattcgtga
    30241 agaaaaaata gtttcagctt cacaattaga acagagatta aatgaaattg ttgagcaaaa
    30301 aaaccaagca aaagagaaaa tagctgagat tgatggaaaa ataaaaaatt ataacaatgt
    30361 cgcaaaactt ttagtaactt acgaaaaata tttaccgatc atgaatgaaa ttgagcgtgc
    30421 tacgatgctt caaaaaatta aattagaaca aaagtacaaa gaagaagtat gccaatttaa
    30481 atttgctgaa aagaaattac tagcttcaga taatctacgt cctgatttaa cgaaagaaaa
    30541 gataatatct gtggcgaaaa atcaagagaa acagaggaaa cgacttatag agggctataa
    30601 gcattatgaa aataaattaa atagattgat cgatgttcaa gaaatattgc aagaactaga
    30661 tagaaatgga ttatttgatc ttagtggtta tcgtttgatt aatagagaag aaaagctaac
    30721 ggacaagatc gagaagtatg aaaaagaagt agaagaaact caacaaaaaa attctaaaga
    30781 taagagtaag gaacgtgaaa tataaataat tgaaaacact atatattccg aaaagtgaat
    30841 atatctcttc ataaaataat caacatattt aaaaaattca atcagccttt tttgtcgaga
    30901 taaagttctt cattctatga tataatgttg ctgtagaaag tttgtggcgg tttggcaaat
    30961 ccaattgaag gagcgtggtg cctatgggct tattagctta tcggacgtac tctgtagaaa
    31021 ggaaatgcct gatttgttct cattggatca aatcattgct attatcggag cagcagcagt
    31081 agtaattact gctatagcga aagctagtga ttcggttttg gcatggttaa agttcaaaaa
    31141 agaacaaaaa aaatagagcc gtctcaactt ttggcgaagt agacgggctc taaacctgat
    31201 caaaaatgcc atccgctttg aacggactac actgggcatg ttacagcatg ccctttcatt
    31261 tttattataa ctcattaatc taaatttgaa aagtaacttt gtttgctaaa gggtgttcta
    31321 catgacaata aacatagcat atatatttta tttttatatt atcaactttg aaaaaattta
    31381 ttctcttcta gaaatagtat gtgaatatat tacaattgaa ggattaattg ctacttcttc
    31441 gatattagta ggattattaa tcccaattgc tattctatta cttgaaaata gtagtaatct
    31501 cattgatcag tctttcaaat gggataaact tgtaattttt tcgcaagtta ttaaaattcg
    31561 ttccattatt ttgggattaa catttataac ttttccttta attttaaatg ataaaactaa
    31621 aaatattttc cctataattt tagccttata tgttatcggg ttatatttta tgttttcact
    31681 tttaaaaaat gcatactatt ggattataag taaagaaaat gaaaaagaat cttatcgtaa
    31741 ttccaaaaga tatcaatatt taaatagtat gactggtaat atagctaatc aaataactat
    31801 ctgggataca atttgggaag atgctgaaca acgaatagga ttagacgagt tgaaacttct
    31861 gaaaattttt tttaaacact gtaaagtagc agaaggtcag aacaagttga atttgttaaa
    31921 tacgtattta cgagattatg aagaagacac tttttttaaa gttacttata gcaattatga
    31981 aataattcaa gattatattt accatgattt atccataata ttaaatgact ctttatctga
    32041 agaatttgga gattatgatt tacttgttgt tattagaaac atgtttttta aatttacaaa
    32101 acaatgctat aatgcttcaa gaataattat atataaatat aatagtaaat ttaattcttt
    32161 tttcattagt tctgactctc gaatactaaa tagactattt ttaaataatt ttgacataag
    32221 atttattgaa actattttaa atagtagatt tactaatgat ataagcctaa aagaaaattt
    32281 accatcctgt tttaactatg ggaatagcaa tttaactgct gaacaccaga acataatagt
    32341 ttcaatgttt agtaaatatt ttgataatta tttaaatagt gctgatcatt atgatgttga
    32401 aaaagtactt cttcttaacg aactattgaa agtagtcttt aacaaagtag atttatcttt
    32461 atatattaca atggaaaatt ttagaaatta ctattctaag aaaattcata accttcttga
    32521 tcttgatgat gactctttta tgataaattt cttcaaagaa tatgcactta tgggagattg
    32581 tgtatctact atttccgaaa atctatttaa agatactgag tggactagga atttgttaat
    32641 gagtatatat atttctcgat atcccatttt tacagatgaa gatcgattaa ataagttaag
    32701 tcaaggtatt tcttctacaa tagaaagtgt tgaattggac acaaaaggac agaaaaaatt
    32761 aaattactta aaacaaaaaa ttgatatgtt gatcaattta taaacaatta gtgtttttgt
    32821 accattagag tatactctaa tggtacaaat ccatatttgc ttaaagcgtt gatttttcag
    32881 cgttttttct ttattgcaaa gtgtacccta agacctgtat actaatagta aatagattgg
    32941 ataggaatga tggcaaatgt atttagctta tttaagagtt tcttccgacg atcaaagttt
    33001 agctcggcaa cataagctaa ttgaaaactg gtcagtcaaa aataatattg ctgaagacga
    33061 tttaaaaatt ttcgaggaaa aagtctctgg aaaaaatatt gaaaacagac cagagctgca
    33121 aaagcttatt tctttcatac gtgaacgaga tacgattatt attcctagtc ttgatcgatt
    33181 agcaagaaat agtaaagata caaaagactt acttcaattg tttcgtagta aaggtgcttc
    33241 tattgaaata ttagacctac cgtcgtttaa tggggtaact gaccctgcat taagagactt
    33301 gctaacaaat ttagtcatag aagtgtttag ctatgttgct gaaaacgaac ggaaaaaaat
    33361 caaagagcgt caaaaacaag gcattgaaat tgcaaagaaa aatggtcgtt atactggtaa
    33421 aaagattcaa taccatgcca atgcgacagg aaaaaacaaa ttgatttatg atgaaattgt
    33481 tgatggtctg aagaaaaaag aaactatttc taaaatagca aagaatgcag gggttacaag
    33541 aaaaactgtt tatcgagtaa aaagagattt aggaatgtaa taagttccag ttagaaagaa
    33601 gcgtaaataa tgcaagaaat agaaatcggt aaaatttatt attgcgaagt tcctagtttg
    33661 gaaggttctt ttgaagttca agttttacaa atgctaaaag acaactgtgt tttagtgaga
    33721 gtcgtacgtt gtacattaat gcaaagtaaa tatttaaaga aaagcaaata tcgttttatt
    33781 gtcaatatca agtccctaaa aggacgatcg ttacactttt aataaagaga tggaggacta
    33841 aataaaaatg aagaataaac tgacattatt aatttgtgca accattttgt caacgtgggc
    33901 agtccctaca caaaatgctt ttgcagaaga aacagggaat gatactacgc agcaaacagt
    33961 gactagcaat agttctagcg aagagcaaac agcaattgta aatggagaag aagtaaaatt
    34021 agaagcgcca gtgaaagaag gacagtctga agctgaagcg actcaagcac caaaagaaga
    34081 gttacaacaa gatcaaatga gtcggtttgc tcgtagtgca gtaagaacaa tacctatgta
    34141 tcgcatgtat aatccaaata gcggagaaca cttctacact caagatactg gagaacgaga
    34201 taatctaaga agaattggtt ggaattacga aggtattggt tggcaagctc caacaagtgg
    34261 cgatccagtt tatcgcttat ataatcgcta taacggggaa catttctata ctttaaatgc
    34321 caaagaacgt gataatatca caaaacaagg gtggacgtat gaaggaattg gctggtactc
    34381 ttataaaggt gctaatggta ttccagttag tcgcttatat aataaacgag taaattggca
    34441 ccactacaca ctagatgaac atgagaaaaa cgtgattagt aagcaaggtt ggaattatga
    34501 aggcgtaggt tggtacgcag tcggcaatgg acaaccaagc aatgacgact ataagcttct
    34561 tggcgtaaag aattacaatc aatatgcttt aggcgctcca agtggctgtg aaggggcttc
    34621 attgctacaa ggattacagt ataaaggaaa gatcacaaat tggagtttaa ctcaattttt
    34681 aaacacgatt cctaaatctc caaatggtaa tccaaacaat gggtttgttg gtagtccatt
    34741 cgttgaaaat tcttggactt actcagctat ttatcctgct ccattagcaa cttgggggca
    34801 aaaatatgga aatgtgcaga atatttcagg tagttctatg aatacattgc ttaatgaagt
    34861 aaagaacgga aatcctgtgg ttgcatgggt aactatcaat ttccaaccaa ttcgttgggg
    34921 aaattggagt tttggtgtag cagctaataa taatcacgca gtgacacttg atggatacaa
    34981 taaaggaagt aatcaagtac atgtatctga tccaattagt ggttcttatt ggctgaatcg
    35041 aacaacattt gaaaatattt acaacgcacg aaaatatgca gttgtagtca gataacattg
    35101 tatgcttcaa gatcatttgt taatattaag gaacggaact cccaaaaatt ccgtactcta
    35161 atttgcagtc tctcactcat ttagagtgag agtttttgtt aaattttcaa attatttaat
    35221 aaagtgatac aatgagttga tcatacaaat caaaagaaga agcagggtaa gagtttacct
    35281 acgcttcttt ttttgtgtgt ttagctattt cttctttaca atatcgaaca tacgtttgta
    35341 taatgtaaaa agaaggggtg ttattcatgt ttgattattc gaaagaacca gtaaatgatt
    35401 attttctaat cgacatgaag tctttttatg cgagtgtcga atgtatagaa agaaatttag
    35461 atccattaac aacagaactt gttgttatga gtcgagctga caatactggt tcaggattga
    35521 tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg agtagaccac
    35581 gtgatttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt atgaaactat
    35641 atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct gatgaagatc
    35701 tactgattta ctcgatcgat gaatcaatct taaaagtgac ccgatcactg aatcttttta
    35761 cgactgaagg aacacgaagt cagcgaagga agaagctcgc tcaaatgatc caagaacgta
    35821 ttaaagaaga gctaggattg attgctgcag taggtgtcgg agataatcct ttgttagcca
    35881 aacttgcctt agacaacgaa gcgaagaaca atgaaggatt tattgccgaa tggacgtatc
    35941 aaaatgttcc tgataaagta tggaatattc ctgaaatgac agacttttgg ggtatcggtt
    36001 ctcgaatgaa aaaaaggctc aaccaaatgg ggatcatgag tatcaaagat ttagctaatt
    36061 ggaatccata caccattaag agccgtttag gcgtgattgg gttacagctg tacttccatg
    36121 caaatggtat tgatcgaact gacatagcaa tcccaccaga accgtcaaag gaaaaatctt
    36181 atggcaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc gaactggtcg
    36241 taaaagaaat ggctgagcaa gtagcaatcc gtattaggca acaccactgt aaaacaggtt
    36301 gtgttcattt acacattgga acgtcgatct tagaaacgag accagggttt tcacaccaga
    36361 tgaaaatacc tatcacagat aatacaagag agctacaaga ctattgtctg ttcttattta
    36421 ataaatacta tgaggggcaa gaagtccgac acgtcggaat aacctatagc aagctagtat
    36481 acactgattc gcttcagtta gatttgttta gtgatccgca aaaacagatt gatgacgaaa
    36541 aactagataa aataatagat aaaattagac aaaaatatgg ttttacgtca attatacacg
    36601 cttcttctaa gcttgaaggt gctagaagta ttacaagaag cacgttagtt ggtggtcatg
    36661 caggcggcgt aggagggctt aaaaatgatt gattacaatg atcgtggacg tttaaagtgg
    36721 gcaggattct atttatcaga tcatactgaa gaaatcgatg ctgagaatga acagcgagcc
    36781 aatcaaaact tagcaaaaga acaaatgaca actgaagaga ttacagaagt attgaacgct
    36841 gcagtcataa aaaatagatc agtatgtatt caaaaagaag aacgaaatgc tgaaggatac
    36901 tatccagatg atattatcgg taaaattttt ggaagtgatg agctaagaat ctatgtcgga
    36961 aaaaacaaag ttcactacga tgaaatacgt catattgaat tttaccagaa acataaatgg
    37021 agctgtttag aatgaacatt ataaagcaat atgagcttaa gtatataaca cttgaaaatc
    37081 tttcagatga aatctgggga tatggtcaac aactaattaa tgaaatagga atcgactgtt
    37141 ttaagtttta cgtattaata gctggtggat acttaaattt tgaatatcat atattgaatt
    37201 atagtgattc tcacttttcg gtataaataa aaaggataga aaaacttcta tcctttttat
    37261 agttttataa acttataaat tcataatttt ataagtttat aattttataa attaattatc
    37321 ggtatcttta tagatgtttt gcactgcttt tatactattt tttacaattt ttttatttgc
    37381 tggattcatg ttgtttatat aaaattctac aattttattt aacgtttcat atattttttt
    37441 atcttcaatt gtcgcaatcg tgctgattgc agtgtgagta tctagtggca ttcttataga
    37501 tttaggtaaa tctttagttg tgagtcgttt tttggattgt tctagaattt tgcctgaatc
    37561 agttgtatta ttcaaggctt cagaattttc tggaacaata ttttcaggtc gttgtgtttt
    37621 attttttctt tttatggcat ttaaaccaat tgattcattt ttttcattag tcatttttaa
    37681 aagctcctta tcctaaaaga ttaatacgtt gtattacttc gtcactaaca ctttgataaa
    37741 cttgttggac tcttttatca tgaatgtctt ttgggttatc ggttatgcca gttctatcat
    37801 accttttcaa tctctgcatt gttttgattt ttgtattgaa aatattcgat tttccaaata
    37861 atgactctgc gtcttcaata atatcgaggt ctaaatcatt accgttttga actagtaccg
    37921 gtagcaagcc taaaatatct atttctgttc cataatcatt gtttaattga attaaatgtt
    37981 ctatatattt ttcagctcct tttaacgagc gttcctgtgt ttgcagaatt acgataacat
    38041 agtcacttgc gactaaagca gaattagtaa atttatttaa ttgtggtggt acgtcaatga
    38101 taataaaatc atagttctct ttaatttcat ttaataaatt tgagaaatgc gtatctttag
    38161 aataatcatc gtcatataat tccgctaaat aatcaatata cttttctaaa tcagtaaatg
    38221 atggtaatag atctaaattt tctttcactg agattagtga attagaaatt ttattatttt
    38281 gaatagaaac aaataatgtt tctttaaatt caggctttat tttgtataca ttggtcatag
    38341 tcgcaaataa tagctctgta gcattagctt gcggatcaag atcaacaact aagactttgt
    38401 aacccttgtt agataatgta taagctgtta acacagcatt agttgtttta ccaacaccac
    38461 ctttaaaatt ccctgtggtt aatacttttg tcatttgtta atacctcctg tttataattt
    38521 tataatttta taaaaatatt atactgctat cgaaataaaa tgtaaacagg aata
//
LOCUS       DACEJR010000020        38324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEJR010000020 DACEJR010000000
VERSION     DACEJR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.020"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            151..381
                     /locus_tag="IW651_001043"
     CDS             151..381
                     /locus_tag="IW651_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0813787.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            478..1779
                     /gene="murA"
                     /locus_tag="IW651_001044"
     CDS             478..1779
                     /gene="murA"
                     /locus_tag="IW651_001044"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0813788.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1792..1968
                     /locus_tag="IW651_001045"
     CDS             1792..1968
                     /locus_tag="IW651_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0813789.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2103..2399
                     /gene="yidD"
                     /locus_tag="IW651_001046"
     CDS             2103..2399
                     /gene="yidD"
                     /locus_tag="IW651_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0813790.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2583..3050
                     /locus_tag="IW651_001047"
     CDS             2583..3050
                     /locus_tag="IW651_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813791.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3135..4019
                     /locus_tag="IW651_001048"
     CDS             3135..4019
                     /locus_tag="IW651_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0813792.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4136..4369
                     /locus_tag="IW651_001049"
     CDS             4136..4369
                     /locus_tag="IW651_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0813793.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4457..4537
                     /locus_tag="IW651_001050"
     tRNA            4457..4537
                     /locus_tag="IW651_001050"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4491..4493,aa:Tyr,seq:gta)
     gene            4544..4615
                     /locus_tag="IW651_001051"
     tRNA            4544..4615
                     /locus_tag="IW651_001051"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4576..4578,aa:Gln,seq:ttg)
     gene            5130..5837
                     /locus_tag="IW651_001052"
     CDS             5130..5837
                     /locus_tag="IW651_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0813794.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5897..6541)
                     /locus_tag="IW651_001053"
     CDS             complement(5897..6541)
                     /locus_tag="IW651_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0813795.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6668..6955
                     /locus_tag="IW651_001054"
     CDS             6668..6955
                     /locus_tag="IW651_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813796.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7063..7383
                     /locus_tag="IW651_001055"
     CDS             7063..7383
                     /locus_tag="IW651_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0813797.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7464..8777
                     /locus_tag="IW651_001056"
     CDS             7464..8777
                     /locus_tag="IW651_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0813798.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8774..9493
                     /locus_tag="IW651_001057"
     CDS             8774..9493
                     /locus_tag="IW651_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0813799.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9621..10172
                     /gene="raiA"
                     /locus_tag="IW651_001058"
     CDS             9621..10172
                     /gene="raiA"
                     /locus_tag="IW651_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0813800.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10394..12928
                     /gene="secA"
                     /locus_tag="IW651_001059"
     CDS             10394..12928
                     /gene="secA"
                     /locus_tag="IW651_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0813801.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13054..14155
                     /gene="prfB"
                     /locus_tag="IW651_001060"
     CDS             join(13054..13116,13118..14155)
                     /gene="prfB"
                     /locus_tag="IW651_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0813802.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14260..14946
                     /gene="ftsE"
                     /locus_tag="IW651_001061"
     CDS             14260..14946
                     /gene="ftsE"
                     /locus_tag="IW651_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0813803.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14930..15823
                     /locus_tag="IW651_001062"
     CDS             14930..15823
                     /locus_tag="IW651_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813804.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15975..17423
                     /gene="cls"
                     /locus_tag="IW651_001063"
     CDS             15975..17423
                     /gene="cls"
                     /locus_tag="IW651_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0813805.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17760..18617
                     /locus_tag="IW651_001064"
     CDS             17760..18617
                     /locus_tag="IW651_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0813806.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18732..19652
                     /gene="pstC"
                     /locus_tag="IW651_001065"
     CDS             18732..19652
                     /gene="pstC"
                     /locus_tag="IW651_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0813807.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19652..20536
                     /gene="pstA"
                     /locus_tag="IW651_001066"
     CDS             19652..20536
                     /gene="pstA"
                     /locus_tag="IW651_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0813808.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20551..21357
                     /gene="pstB"
                     /locus_tag="IW651_001067"
     CDS             20551..21357
                     /gene="pstB"
                     /locus_tag="IW651_001067"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813809.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21373..22131
                     /locus_tag="IW651_001068"
     CDS             21373..22131
                     /locus_tag="IW651_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813810.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQVSRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22144..22821
                     /gene="phoU"
                     /locus_tag="IW651_001069"
     CDS             22144..22821
                     /gene="phoU"
                     /locus_tag="IW651_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0813811.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23059..24090
                     /locus_tag="IW651_001070"
     CDS             23059..24090
                     /locus_tag="IW651_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0813812.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24087..24854
                     /locus_tag="IW651_001071"
     CDS             24087..24854
                     /locus_tag="IW651_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0813813.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24954..26522
                     /gene="liaX"
                     /locus_tag="IW651_001072"
     CDS             24954..26522
                     /gene="liaX"
                     /locus_tag="IW651_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0813814.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26822..27139
                     /locus_tag="IW651_001073"
     CDS             26822..27139
                     /locus_tag="IW651_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0813815.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27139..27489
                     /locus_tag="IW651_001074"
     CDS             27139..27489
                     /locus_tag="IW651_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0813816.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27656..28594
                     /locus_tag="IW651_001075"
     CDS             27656..28594
                     /locus_tag="IW651_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0813817.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28609..29439
                     /locus_tag="IW651_001076"
     CDS             28609..29439
                     /locus_tag="IW651_001076"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0813818.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29483..30508
                     /locus_tag="IW651_001077"
     CDS             29483..30508
                     /locus_tag="IW651_001077"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0813819.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30523..31461
                     /gene="galU"
                     /locus_tag="IW651_001078"
     CDS             30523..31461
                     /gene="galU"
                     /locus_tag="IW651_001078"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0813820.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31586..32026
                     /locus_tag="IW651_001079"
     CDS             31586..32026
                     /locus_tag="IW651_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0813821.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32029..32631
                     /locus_tag="IW651_001080"
     CDS             32029..32631
                     /locus_tag="IW651_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0813822.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32694..33797)
                     /locus_tag="IW651_001081"
     CDS             complement(32694..33797)
                     /locus_tag="IW651_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0813823.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34007..35008
                     /gene="ccpA"
                     /locus_tag="IW651_001082"
     CDS             34007..35008
                     /gene="ccpA"
                     /locus_tag="IW651_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0813824.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35363..35758
                     /locus_tag="IW651_001083"
     CDS             35363..35758
                     /locus_tag="IW651_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813825.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35758..36009
                     /locus_tag="IW651_001084"
     CDS             35758..36009
                     /locus_tag="IW651_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813826.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36052..36447
                     /locus_tag="IW651_001085"
     CDS             36052..36447
                     /locus_tag="IW651_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813827.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36444..37700
                     /locus_tag="IW651_001086"
     CDS             36444..37700
                     /locus_tag="IW651_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0813828.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37712..38035
                     /locus_tag="IW651_001087"
     CDS             37712..38035
                     /locus_tag="IW651_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813829.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38028..38294
                     /locus_tag="IW651_001088"
     CDS             38028..38294
                     /locus_tag="IW651_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813830.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12858 a         6511 c         8259 g        10696 t
ORIGIN      
        1 tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat
       61 ttttaagtat acttgttttt ccttttatac attttatggt attatcattt tattagttat
      121 tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt
      181 cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc
      241 attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt
      301 tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc tttatgccga
      361 aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta
      421 taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg
      481 gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct
      541 aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca
      601 ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta
      661 aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc ttcaagacaa
      721 ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg
      781 ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt
      841 ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt
      901 caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta
      961 gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga
     1021 acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg
     1081 aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg
     1141 gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt
     1201 atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat
     1261 aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc
     1321 ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat
     1381 cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt
     1441 acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc
     1501 cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa
     1561 ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct
     1621 ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat
     1681 tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt agacatggat
     1741 caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac
     1801 caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt
     1861 ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa
     1921 gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc
     1981 acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa agaaaacaga
     2041 agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat
     2101 tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat
     2161 ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa
     2221 cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt
     2281 ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga aataagaaag
     2341 ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact gagcgataaa
     2401 gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt
     2461 tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact gagcgataaa
     2521 gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa
     2581 aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc
     2641 ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa
     2701 aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca
     2761 atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag
     2821 cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata cgaaccggca
     2881 gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag attggtcgag
     2941 tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa
     3001 gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa taaactttca
     3061 attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa
     3121 aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg
     3181 gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg
     3241 aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg
     3301 aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa
     3361 tcagtgcgat tccttatatt catggattta atattttccg agatttggaa actctgtttc
     3421 aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag
     3481 gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg
     3541 cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga gaatttgggt
     3601 tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt
     3661 ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa gatgtctttg
     3721 aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc
     3781 ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg
     3841 gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga
     3901 cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa tacagaaacg
     3961 atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg gagaattaag
     4021 agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag gagattttta
     4081 cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc
     4141 aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa
     4201 gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta
     4261 tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct
     4321 tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa
     4381 aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg
     4441 ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt
     4501 tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg
     4561 gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac
     4621 cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta ccttgttcct
     4681 tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga gggatccaca
     4741 tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg gtcaaatcag
     4801 aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc caggtaaaat
     4861 caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa taataacaaa
     4921 agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc taggttgaac
     4981 atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt ctaaatcctc
     5041 tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata ccagttataa
     5101 tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta tatatacaga
     5161 ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga gaccgtcttc
     5221 cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg agacaagcaa
     5281 ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt acatatgttg
     5341 cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa attatggaat
     5401 cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg ccaagctcaa
     5461 gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg gaacgtattc
     5521 gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa aagctgatcg
     5581 atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa gaaaaaggcg
     5641 ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct gagaaaattg
     5701 ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag atttcttatt
     5761 ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat cgttttgagt
     5821 tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa taaaagagaa
     5881 tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa gaaacaggac
     5941 cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggtcag
     6001 cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg atttctttta
     6061 taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca
     6121 tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag
     6181 agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat
     6241 atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg
     6301 gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt
     6361 ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt
     6421 gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg
     6481 attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca
     6541 tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc
     6601 aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa ggtaggtgga
     6661 tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa
     6721 tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa
     6781 cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc
     6841 tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga
     6901 caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa
     6961 ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta acagacgcga
     7021 agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa aaaaacaatc
     7081 atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct
     7141 gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga cgcagataac
     7201 aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg ctttatgaaa
     7261 gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat caatatggct
     7321 gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct cataggtgaa
     7381 taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg
     7441 tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag
     7501 atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag
     7561 tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg
     7621 atggacagct tgcagattac ggttattttt gtacacattg tctatattta gggcgatgcg
     7681 acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct
     7741 ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc
     7801 attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc
     7861 tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc
     7921 gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa
     7981 tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta
     8041 cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg
     8101 atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca gcgattaaac
     8161 cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta aaggaaatca
     8221 agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta cttcctgaac
     8281 ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag aagataaaat
     8341 ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta ttttgtccaa
     8401 gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg agatatgaga
     8461 tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa atgcgagaag
     8521 caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca tttgaaagaa
     8581 tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct ttggtacaaa
     8641 tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac tttttctatg
     8701 aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg aatgaagaag
     8761 ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa atttaacatt
     8821 cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag tttgtcgcgc
     8881 acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa tccatttcaa
     8941 tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg actgtcaaga
     9001 atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg tgtacgatga
     9061 aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac tagcaggcac
     9121 ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct attgtccaat
     9181 acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg agatgttgaa
     9241 agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc tggctcaagc
     9301 aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg cagtcacctt
     9361 ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa caggacagac
     9421 tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc gaaccatttc
     9481 tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa ataacctaga
     9541 gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt
     9601 agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa
     9661 ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg
     9721 acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag
     9781 tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt
     9841 tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa
     9901 caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct actgctttat
     9961 tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc
    10021 tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata
    10081 acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag
    10141 atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac
    10201 ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa
    10261 atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa
    10321 ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga
    10381 gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact
    10441 aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt
    10501 aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga
    10561 aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt
    10621 attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa
    10681 tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct
    10741 aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg
    10801 tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat
    10861 caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac
    10921 gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat
    10981 ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga
    11041 agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag
    11101 agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc
    11161 aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga
    11221 taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc
    11281 caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt
    11341 tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc
    11401 aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac
    11461 tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac
    11521 agaagaagaa gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg
    11581 tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca
    11641 cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac
    11701 tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca
    11761 tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca
    11821 aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg
    11881 acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg
    11941 tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca
    12001 gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaatc
    12061 tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac
    12121 gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa
    12181 cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca
    12241 agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac
    12301 aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag
    12361 tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc ttgcagatat
    12421 cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt
    12481 taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt
    12541 gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg
    12601 tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga
    12661 aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt
    12721 tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc
    12781 tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat
    12841 ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa
    12901 gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct
    12961 ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta gactagatat
    13021 aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc
    13081 ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact tagaccagct
    13141 ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgatag
    13201 cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt atgatcaatt
    13261 ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt tgcaggaaga
    13321 acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag atgaaaaaat
    13381 gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca acgccatcgt
    13441 tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta tgcttttacg
    13501 tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag attatcaagc
    13561 aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca acgcatttgg
    13621 ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt ttgactcagc
    13681 gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat
    13741 cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg
    13801 tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac
    13861 agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag cgatggggat
    13921 gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact
    13981 tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc
    14041 atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat
    14101 ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc
    14161 cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct
    14221 ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta
    14281 atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt catcgaacaa
    14341 ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg
    14401 atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata
    14461 gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac
    14521 tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc
    14581 ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct tgttggtttg
    14641 aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct
    14701 attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat
    14761 ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga
    14821 actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta
    14881 attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat
    14941 gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg
    15001 gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg gtgtattcat
    15061 ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg ttgatgtctc
    15121 tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa
    15181 tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat
    15241 ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga
    15301 tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc
    15361 agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca
    15421 aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc
    15481 gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca
    15541 aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg
    15601 tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat ttggttatgc
    15661 atggtttttc caattgatca atccatcatt attacgttcc cattattcat tgattcatcc
    15721 acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc
    15781 acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa
    15841 aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa
    15901 ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa
    15961 gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga
    16021 atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg
    16081 catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag
    16141 gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga
    16201 acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta
    16261 ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct
    16321 atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga
    16381 ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg
    16441 atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga gggggaaaag
    16501 tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt
    16561 tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc
    16621 ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa atcggatata
    16681 caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc
    16741 gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga
    16801 tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca
    16861 ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg
    16921 aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc
    16981 aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt
    17041 caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg
    17101 caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa acatatgaga
    17161 atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag
    17221 caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg
    17281 aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc
    17341 tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa
    17401 gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag
    17461 atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt
    17521 ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa
    17581 gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag aaacttttaa
    17641 tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat tttgattcgt
    17701 tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg agatttctaa
    17761 tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta tcaggctgtt
    17821 caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct ttacagcctt
    17881 tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc atcaatgtac
    17941 aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc gacatcggaa
    18001 actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta gtcgatcatc
    18061 gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta aaagacatca
    18121 gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt gaagtcggtg
    18181 gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc cgtagcacgt
    18241 ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa gattcaagcg
    18301 gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt gctttttctt
    18361 atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca gatgaaaatg
    18421 tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga gatcatccaa
    18481 aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa gataacatcg
    18541 ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat tggcaaggaa
    18601 atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact tttacatgtt
    18661 attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac ctgattaagg
    18721 gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa cgttcaaaaa
    18781 tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg ttggttgtat
    18841 tatctatttt ttattttgta gctagtaaag gattggccac cttttttact gataagatca
    18901 atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga gcaaatggga
    18961 aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc tttttatctg
    19021 ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag atctctccta
    19081 aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt ccttctgtcg
    19141 tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt tttggcggga
    19201 ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg ccgactgtca
    19261 caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa gcttcattag
    19321 cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca gccgtgcccg
    19381 ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc ttagctatcc
    19441 aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct gcttctacat
    19501 tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt gaaaataatg
    19561 tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat atcgtgatcc
    19621 gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag ataaaattgc
    19681 tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag ctttactact
    19741 ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg aaccttccaa
    19801 agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt atttgctttt
    19861 gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc tttcagaata
    19921 tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct tgagctcgtt
    19981 gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat tccagtatgg
    20041 tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc ttttgactag
    20101 aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag gattggcctt
    20161 aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga caccagggat
    20221 tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg cattgatcta
    20281 tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat taagtgtttc
    20341 aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt ggaagatcaa
    20401 tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg ccgtgttgat
    20461 tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat tataccgaaa
    20521 aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa tgaaacaaat
    20581 gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct acatgtctgg
    20641 tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa taagatcact
    20701 gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt gaatcgaatg
    20761 aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg cgtagatatt
    20821 aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt ttttcaacgt
    20881 cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa acagcacggc
    20941 gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca agctgccttg
    21001 tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg cgggcaacag
    21061 caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct gctagacgag
    21121 ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact tgtaaacttg
    21181 aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc tcgtatcagc
    21241 gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac taacaagata
    21301 tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt tggttaagac
    21361 ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct gtattatgga
    21421 aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat cactgcgatg
    21481 atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg gatgaatgac
    21541 ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga catctatggt
    21601 ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca gcagccaaac
    21661 ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa aggggaaaaa
    21721 gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc tgtttgggat
    21781 gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca gcagcagcgt
    21841 gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga tgaaccaaca
    21901 agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga attaagagag
    21961 cggtatacga tgatcatcgt gacccataat atgtctcaag tttcacgtat ttcagataag
    22021 acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa gatattcatg
    22081 aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata aggaggaaac
    22141 ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca gttctacgaa
    22201 atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat caaacacgat
    22261 aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat ggaaacacgc
    22321 ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac tgatttgcgt
    22381 atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga tcatgcggtg
    22441 tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga aatcgaaaaa
    22501 gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct agttgcttat
    22561 gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag agtcaatgac
    22621 tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc tgagacagtc
    22681 atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg agactatgtg
    22741 acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga gttaaatacg
    22801 aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta ggaaaagagg
    22861 ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagtcaaaca
    22921 aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg caagcttttg
    22981 aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct tttcatcatt
    23041 tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt ttttatttgt
    23101 gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc tgtcatcgag
    23161 atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact gatgaacacg
    23221 gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca aatagaaaca
    23281 gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga atggcagcta
    23341 gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat tggcgccatg
    23401 ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat caatatcgtt
    23461 ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct attaaatata
    23521 tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga tggatggaat
    23581 agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc attttttatt
    23641 tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga actgaattat
    23701 ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac agctggagga
    23761 acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa gcgacaacca
    23821 gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga tgtgattggc
    23881 tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc gtttctttta
    23941 tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc attggaattt
    24001 ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc ggtcctcttt
    24061 tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt gggattttag
    24121 caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta ggcttattca
    24181 tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg ccgatactgg
    24241 cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt atcaacggtt
    24301 ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt tgtttcctac
    24361 tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta gaactagaag
    24421 aattatctgg cttagataag actttagcga ttgattttag attacttacg agatcgttgc
    24481 ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct agtgggacat
    24541 tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat tctagtttcg
    24601 ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca ttcatgggaa
    24661 gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag caagtgatca
    24721 atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg tccgcactac
    24781 ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag aaagaacaac
    24841 tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa tcagtgcata
    24901 atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag
    24961 aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag
    25021 atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa gaagcagaca
    25081 aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat aaacttgaaa
    25141 acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga caagatcaag
    25201 aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact tcggcgcgtc
    25261 tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag aaacaggaag
    25321 cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa gaattagcac
    25381 agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat acactgttag
    25441 aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa tggtctgaga
    25501 aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat caagcaacag
    25561 atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta ggcaaattct
    25621 tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa tggaaagatg
    25681 tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt tactatgaag
    25741 ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt
    25801 gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa atgggtgctg
    25861 aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat catatctcct
    25921 tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct aaacgagtat
    25981 acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca ttggaagcta
    26041 aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta actgcaacga
    26101 tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac atcttagatt
    26161 ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa aacctttctg
    26221 tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg aaaaaagtag
    26281 aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc ggtttggaag
    26341 gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac gaagtagttc
    26401 gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca gatggtgaaa
    26461 tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat acagataaat
    26521 aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt gtaaaatagc
    26581 ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga acaccgttta
    26641 ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt cagaaaaagc
    26701 tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa gacaccgatt
    26761 aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga ggtgagacat
    26821 gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga ctttagcagg
    26881 aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg tattcctgag
    26941 cttagttgct tttggaagtc caattatttt atacatttta ttagctttga ttatcccatc
    27001 tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa aaaacaatta
    27061 tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga
    27121 cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac gttaacattc
    27181 atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg gacggccgtt
    27241 cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt gacgatactg
    27301 tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa tgcagcgatt
    27361 cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc attcggtgca
    27421 gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga acataacagt
    27481 agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta gaatcaggaa
    27541 tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg tagtactttt
    27601 ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag catgaatgtc
    27661 agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg tgacaggcga
    27721 cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag gactagagtt
    27781 gactggttat tttaattatt attcccataa tcgtttacag ctatttggca gtaaagaaat
    27841 cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa gattatgtag
    27901 tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag aaatgatcca
    27961 ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt ctcgtttatt
    28021 aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg tccacggtgt
    28081 tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga taggaaaaag
    28141 tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg atcgagtaga
    28201 tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc tggaacatct
    28261 gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg cgagtgcggt
    28321 acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa aagataagaa
    28381 atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg gcattccaca
    28441 aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag tagccgcgat
    28501 gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag aaagattaag
    28561 tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat gttagcacaa
    28621 atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc attgatcatc
    28681 gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg ggtaggatta
    28741 aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat tattggcgct
    28801 cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat ccaaatattc
    28861 aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc gatcacgctg
    28921 tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat cgctgcacca
    28981 agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca cgaagcatat
    29041 ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt catcattgat
    29101 aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc actttggagt
    29161 ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt gaaaaaaggc
    29221 gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat tgaaggatta
    29281 cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt atctgctgtg
    29341 ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa tttaccggat
    29401 tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg ttaaaaatga
    29461 aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac caggctcttg
    29521 gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga tttggggaca
    29581 tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt atttaccaga
    29641 tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag taaaagacgc
    29701 agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa aagaactagt
    29761 gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag aacagggaac
    29821 acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa gaaaagctgt
    29881 tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca tcactacgat
    29941 cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt tcatgaataa
    30001 ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg cagctttgaa
    30061 aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg ataatgcaaa
    30121 ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag caatgggtgc
    30181 caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta cttgcacaag
    30241 tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc ccctagaaga
    30301 ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag cagcgatgga
    30361 actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg aagtgctgta
    30421 tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg gaaaaaaaga
    30481 aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt taaaaaagca
    30541 gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc tatggcaaag
    30601 gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga agcgttgaaa
    30661 tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat cgaagatcac
    30721 tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac cgaattattg
    30781 aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc tcatccaaag
    30841 ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc atttgtcgtt
    30901 atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt gatcgatgac
    30961 tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga tgaaacttca
    31021 aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa tgtcaaaaac
    31081 tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat tgggcgttac
    31141 ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc tggaaatgag
    31201 atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt tgcccgtaaa
    31261 ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac aagtattgaa
    31321 tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat gtctttaggt
    31381 aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat aacagaaatc
    31441 aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagcggcta attgaataaa
    31501 aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc cttatagaaa
    31561 agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat tgatcgcagc
    31621 aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag tttccaaaac
    31681 tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga tcgtcacaca
    31741 agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta atgagctgct
    31801 cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag ttgctgattt
    31861 aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga aagtcggcgg
    31921 aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa cagcgatgaa
    31981 attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat ggctaaaaaa
    32041 ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc cgcattattg
    32101 ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc agatgatttg
    32161 atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga gttaacaggt
    32221 attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc aggcaatgtt
    32281 aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga tatcttgtta
    32341 aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc agaagatctg
    32401 aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga tatcatgatc
    32461 gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat gtcagaagca
    32521 aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga tgcaaaagaa
    32581 gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta agattactca
    32641 tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt tcattactct
    32701 aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact tgtcacatac
    32761 acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg ttcaagagag
    32821 aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg gaattcgtgg
    32881 acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta gccatagctg
    32941 ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg gcgaactgcc
    33001 tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg gaactcagtt
    33061 ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca aataacgcca
    33121 agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt accatgaggg
    33181 cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga aacaccttgt
    33241 ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc ttcttttact
    33301 gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat cagtttgtct
    33361 ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag atcggtgtaa
    33421 gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt cagagcattc
    33481 ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt tccgatgatc
    33541 tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct gctgttttga
    33601 gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag agggataaaa
    33661 agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc gatgtggcca
    33721 gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg tagttcattg
    33781 atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt caataattgt
    33841 accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa attttcatga
    33901 aaacagagat aaaacggttg caattgttga gattatttga tatcctattt tagaaatgta
    33961 agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg aaaagcaaac
    34021 aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg tttcacgtgt
    34081 cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg aagttatcga
    34141 tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga aaacaacaac
    34201 agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag cacgtggtat
    34261 cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag atggcaacga
    34321 tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg gcgtgatctt
    34381 tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa aaacgcctgt
    34441 tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca ttgattatgt
    34501 gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa aaatcgcttt
    34561 tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag gctataaaga
    34621 agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg cacaatacaa
    34681 ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa cagctgtcta
    34741 tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc gcggcgtaaa
    34801 agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag aagtagctcg
    34861 tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat ccatgcgcct
    34921 attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct tgccttatgg
    34981 aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg ttgttccaaa
    35041 agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa aattttgtca
    35101 taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa accttacgtc
    35161 catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact atcaaaatta
    35221 ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa ctctaaaaaa
    35281 tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat ccatcatata
    35341 taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag tcatcatttt
    35401 gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag aaatgcaaag
    35461 acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa atacctttgc
    35521 ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg gttcataccg
    35581 gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt atagtttttg
    35641 gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc aaaaagaagg
    35701 aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa gtatttaatg
    35761 attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga tcctaaacat
    35821 agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg cactatgtat
    35881 aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt agcgccaatc
    35941 gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa ctttcgcgta
    36001 cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt cttgtcaagg
    36061 aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat cggagggaaa
    36121 gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt agaaaaacag
    36181 tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt agaagaattt
    36241 aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa taatgaaggg
    36301 aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg tggttttggc
    36361 attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa agtaattttt
    36421 tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat gatattagtg
    36481 agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac aaaaagaact
    36541 ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac tcattggacg
    36601 gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt tctcaagtag
    36661 tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg gatgcaagaa
    36721 gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa gcaacaacaa
    36781 acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca gcgattgcat
    36841 ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc ggccattcat
    36901 taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat gtcggcttca
    36961 acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg gaagaaaatc
    37021 ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt ggtggtaatg
    37081 gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg cttgcttccc
    37141 acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct gaaaatgaaa
    37201 acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg ttttcctatc
    37261 agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc cagctgacag
    37321 ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa aagttatcgg
    37381 caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga atttttgagc
    37441 tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct tcgttgactg
    37501 aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac ttagtcgatt
    37561 ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg gaggcctttg
    37621 atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga gatcgagaat
    37681 tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg aaagagatta
    37741 tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact ttaagaatca
    37801 aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa agaacttagc
    37861 tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc aggagcatta
    37921 tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg aactagaacg
    37981 ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg aatgaatttt
    38041 taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta cgttcagttg
    38101 gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa aactaccaaa
    38161 tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg aaaggatcag
    38221 taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa aagaaattaa
    38281 aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaat
//
LOCUS       DACEJR010000021        38242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEJR010000021 DACEJR010000000
VERSION     DACEJR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.021"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(66..506)
                     /locus_tag="IW651_001089"
     CDS             complement(66..506)
                     /locus_tag="IW651_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0813831.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(563..973)
                     /locus_tag="IW651_001090"
     CDS             complement(563..973)
                     /locus_tag="IW651_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0813832.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(1153..1587)
                     /locus_tag="IW651_001091"
     CDS             complement(1153..1587)
                     /locus_tag="IW651_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0813833.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(1687..2535)
                     /locus_tag="IW651_001092"
     CDS             complement(1687..2535)
                     /locus_tag="IW651_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0813834.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            2924..4690
                     /locus_tag="IW651_001093"
     CDS             2924..4690
                     /locus_tag="IW651_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0813835.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            4792..6051
                     /locus_tag="IW651_001094"
     CDS             4792..6051
                     /locus_tag="IW651_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0813836.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            6198..7499
                     /locus_tag="IW651_001095"
     CDS             6198..7499
                     /locus_tag="IW651_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813837.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            7502..8353
                     /locus_tag="IW651_001096"
     CDS             7502..8353
                     /locus_tag="IW651_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813838.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            8366..9181
                     /locus_tag="IW651_001097"
     CDS             8366..9181
                     /locus_tag="IW651_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0813839.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            9332..10498
                     /locus_tag="IW651_001098"
     CDS             9332..10498
                     /locus_tag="IW651_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0813840.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            10728..11375
                     /locus_tag="IW651_001099"
     CDS             10728..11375
                     /locus_tag="IW651_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0813841.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            11459..12403
                     /locus_tag="IW651_001100"
     CDS             11459..12403
                     /locus_tag="IW651_001100"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0813842.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKVNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            12538..13989
                     /locus_tag="IW651_001101"
     CDS             12538..13989
                     /locus_tag="IW651_001101"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0813843.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            14010..14846
                     /locus_tag="IW651_001102"
     CDS             14010..14846
                     /locus_tag="IW651_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0813844.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            14846..15505
                     /locus_tag="IW651_001103"
     CDS             14846..15505
                     /locus_tag="IW651_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0813845.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            15631..16320
                     /locus_tag="IW651_001104"
     CDS             15631..16320
                     /locus_tag="IW651_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813846.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            16326..17282
                     /locus_tag="IW651_001105"
     CDS             16326..17282
                     /locus_tag="IW651_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0813847.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            17326..17838
                     /locus_tag="IW651_001106"
     CDS             17326..17838
                     /locus_tag="IW651_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0813848.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(18094..18642)
                     /locus_tag="IW651_001107"
     CDS             complement(18094..18642)
                     /locus_tag="IW651_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0813849.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(18731..19912)
                     /locus_tag="IW651_001108"
     CDS             complement(18731..19912)
                     /locus_tag="IW651_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0813850.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(20148..21656)
                     /locus_tag="IW651_001109"
     CDS             complement(20148..21656)
                     /locus_tag="IW651_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0813851.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            21815..22006
                     /locus_tag="IW651_001110"
     CDS             21815..22006
                     /locus_tag="IW651_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813852.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            22209..23126
                     /locus_tag="IW651_001111"
     CDS             22209..23126
                     /locus_tag="IW651_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0813853.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            23119..24192
                     /locus_tag="IW651_001112"
     CDS             23119..24192
                     /locus_tag="IW651_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813854.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            24340..24858
                     /locus_tag="IW651_001113"
     CDS             24340..24858
                     /locus_tag="IW651_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813855.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            24839..25231
                     /locus_tag="IW651_001114"
     CDS             24839..25231
                     /locus_tag="IW651_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0813856.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            25307..26242
                     /locus_tag="IW651_001115"
     CDS             25307..26242
                     /locus_tag="IW651_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0813857.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            26375..27154
                     /locus_tag="IW651_001116"
     CDS             26375..27154
                     /locus_tag="IW651_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0813858.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            27147..27464
                     /locus_tag="IW651_001117"
     CDS             27147..27464
                     /locus_tag="IW651_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0813859.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            27708..29318
                     /locus_tag="IW651_001118"
     CDS             27708..29318
                     /locus_tag="IW651_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0813860.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            29475..30323
                     /locus_tag="IW651_001119"
     CDS             29475..30323
                     /locus_tag="IW651_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0813861.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            30554..31033
                     /locus_tag="IW651_001120"
     CDS             30554..31033
                     /locus_tag="IW651_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813862.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            31066..33294
                     /locus_tag="IW651_001121"
     CDS             31066..33294
                     /locus_tag="IW651_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0813863.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            33423..35066
                     /locus_tag="IW651_001122"
     CDS             33423..35066
                     /locus_tag="IW651_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0813864.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35139..35684)
                     /locus_tag="IW651_001123"
     CDS             complement(35139..35684)
                     /locus_tag="IW651_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0813865.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(35707..36192)
                     /locus_tag="IW651_001124"
     CDS             complement(35707..36192)
                     /locus_tag="IW651_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0813866.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(36182..36994)
                     /locus_tag="IW651_001125"
     CDS             complement(36182..36994)
                     /locus_tag="IW651_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0813867.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(37133..>38242)
                     /locus_tag="IW651_001126"
     CDS             complement(37133..>38242)
                     /locus_tag="IW651_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0813868.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        12512 a         6923 c         7536 g        11271 t
ORIGIN      
        1 aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag
       61 cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt
      121 tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata
      181 ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt
      241 tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag
      301 ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt
      361 ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg
      421 aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact
      481 tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca
      541 tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc
      601 cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt
      661 cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt
      721 cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt
      781 gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt
      841 gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg
      901 tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat
      961 acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa
     1021 tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa
     1081 acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc
     1141 tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc
     1201 acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt
     1261 cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc
     1321 tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg
     1381 ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt
     1441 accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag
     1501 ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac
     1561 aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta
     1621 ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt
     1681 accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct
     1741 gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc
     1801 atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc
     1861 ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa
     1921 ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt
     1981 catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa
     2041 aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt
     2101 caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt
     2161 gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa
     2221 caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaccaa
     2281 gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa
     2341 cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag cagcttccag
     2401 taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt
     2461 gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg
     2521 tctcgtttct ttcatttatt cacttcctta attcattaat aatactctta tcttattata
     2581 acttactttt caccttaagt taactttaat taataaaaaa attaggctag tctaaattat
     2641 tatgtttcat tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa cttttacttt
     2701 agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag tacaggaagt
     2761 agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt
     2821 cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg cttctctatc
     2881 acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat
     2941 ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg
     3001 gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag atccatatac
     3061 cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa
     3121 tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt
     3181 ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta
     3241 tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat
     3301 cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga
     3361 aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa
     3421 agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg atcatttgga
     3481 ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta aagccacatc
     3541 caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa
     3601 acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc
     3661 tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga
     3721 aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat
     3781 gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt
     3841 catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac
     3901 ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga
     3961 tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat
     4021 ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt
     4081 gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga aaatcacagc
     4141 ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga
     4201 aggaacattc aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg
     4261 taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg
     4321 tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg
     4381 tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt
     4441 ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt
     4501 aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga tcatctatgt
     4561 ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag
     4621 tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat
     4681 catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc
     4741 ccattataat aagggtgtaa cagcacgtag aatacaaagg aggaaatgaa gatgaaaaaa
     4801 agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg
     4861 gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca
     4921 gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa
     4981 gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta
     5041 gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc
     5101 ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac
     5161 gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta
     5221 atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt
     5281 aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa
     5341 aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt
     5401 tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat
     5461 gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag
     5521 ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa
     5581 actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat
     5641 tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga
     5701 ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt
     5761 gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct
     5821 gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg
     5881 attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg
     5941 acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc
     6001 gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga
     6061 taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag
     6121 atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt
     6181 ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca
     6241 cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta
     6301 tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg
     6361 cattggtagg attggttact ttgggaagta taccaatgga agatcattca ttatttattt
     6421 tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga
     6481 atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga
     6541 cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc
     6601 ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg
     6661 catttactaa ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg
     6721 ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct
     6781 tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc
     6841 tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt
     6901 tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca
     6961 acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg
     7021 tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct
     7081 tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat
     7141 cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct
     7201 tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt
     7261 atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc ggtcccggaa
     7321 gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga
     7381 ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg
     7441 ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac
     7501 aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta
     7561 cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac
     7621 tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt
     7681 aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt
     7741 gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac
     7801 ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt tagtgattca
     7861 gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct tattagaagc
     7921 gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac
     7981 ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact
     8041 tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat
     8101 gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa
     8161 gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc
     8221 tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc
     8281 gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg
     8341 aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt
     8401 acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt
     8461 gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca
     8521 gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg
     8581 aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac
     8641 gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga
     8701 tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc
     8761 tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac
     8821 gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat
     8881 cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt
     8941 attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac
     9001 attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc
     9061 agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac
     9121 tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg
     9181 acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt
     9241 tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg
     9301 taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt
     9361 attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca
     9421 atacggagat acggacactc ctacccaaat caaaatcaat atccatgaag aaatcccaat
     9481 gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg atcatcgaat
     9541 cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt
     9601 tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa
     9661 aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata aactttggct
     9721 agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca
     9781 agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac
     9841 gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa
     9901 tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat
     9961 aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa
    10021 aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa
    10081 tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat
    10141 ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt
    10201 ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt
    10261 gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac
    10321 aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg
    10381 aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt
    10441 gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt
    10501 gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga
    10561 agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat
    10621 attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat tttttcttcg
    10681 ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg
    10741 ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc
    10801 aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact
    10861 cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta
    10921 ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc
    10981 cagatactgc aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga
    11041 tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa
    11101 tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag
    11161 aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg
    11221 acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt
    11281 attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa
    11341 caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg
    11401 atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt
    11461 gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg
    11521 tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt
    11581 tatcactaag agctgtacga tcaatgaacg aaaagtaaat cccgaaccac gctattttga
    11641 tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt cttattatct
    11701 agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc
    11761 cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg
    11821 gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact
    11881 agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa
    11941 gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga
    12001 tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt
    12061 gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat
    12121 tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt
    12181 gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga
    12241 acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata aagaagggcc
    12301 ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac
    12361 gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca
    12421 tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta
    12481 ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg
    12541 aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta
    12601 gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg
    12661 cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca
    12721 ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct
    12781 acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg
    12841 caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc
    12901 aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt
    12961 acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta
    13021 gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt
    13081 gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa
    13141 ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat
    13201 gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca
    13261 catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag
    13321 attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg
    13381 catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat
    13441 cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga
    13501 gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag
    13561 ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg
    13621 gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct
    13681 aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat
    13741 tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc
    13801 acaccttata cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat
    13861 ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat
    13921 ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca
    13981 tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa
    14041 ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca
    14101 gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc
    14161 ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag
    14221 aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa
    14281 tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag
    14341 aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg
    14401 tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag
    14461 ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca
    14521 catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg
    14581 ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg
    14641 taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag
    14701 gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga
    14761 cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa
    14821 cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg
    14881 gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc
    14941 gtttctttta ggaaatctgg tagggttgct tttgacagtt ttaggtcttt tggattggct
    15001 gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt
    15061 cctgctgttt ctgttatatt ttgggttacc ttatcaatta agtgccttaa cggcttcagt
    15121 catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc
    15181 aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt
    15241 attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt
    15301 agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt
    15361 tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct atattttagt
    15421 tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa
    15481 acgttctatg catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt
    15541 tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt
    15601 aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa
    15661 tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg
    15721 cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga
    15781 gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg
    15841 tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa
    15901 actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag
    15961 atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta
    16021 ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca
    16081 aagcaaatgg agcgaggcgt aaaatatttt ccaacagatc aattgtacaa agaattgaaa
    16141 agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag
    16201 gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat
    16261 tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga
    16321 aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag
    16381 cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt
    16441 ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcga atcgctgtga
    16501 tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag
    16561 tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag
    16621 tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag
    16681 aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct
    16741 ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc
    16801 atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc
    16861 acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata
    16921 aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata
    16981 tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt
    17041 ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga
    17101 ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg
    17161 gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc
    17221 atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat
    17281 gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa
    17341 acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt
    17401 gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg
    17461 aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca
    17521 gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa
    17581 tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa
    17641 aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa
    17701 gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct
    17761 ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat
    17821 caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact
    17881 gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac
    17941 aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa
    18001 atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct
    18061 ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt tttttatttt
    18121 atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt
    18181 caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc
    18241 agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa
    18301 gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa
    18361 aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata
    18421 tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa
    18481 tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg
    18541 attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt
    18601 tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat
    18661 gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct
    18721 tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg
    18781 ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc
    18841 ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga
    18901 cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca
    18961 tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt
    19021 ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt
    19081 ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa
    19141 aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc
    19201 ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt
    19261 cttctaatac atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa
    19321 caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca
    19381 tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg
    19441 gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc
    19501 cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt
    19561 tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata
    19621 caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa
    19681 aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata
    19741 cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta
    19801 aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa
    19861 aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac
    19921 tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg
    19981 aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa
    20041 caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat
    20101 cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta ttggtaatat
    20161 agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt
    20221 tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc aagttcagct
    20281 agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga taaagcgatc
    20341 aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct
    20401 tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat atgggctaaa
    20461 tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact tgtacgcccg
    20521 gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc ttttgtagga
    20581 ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt tcggtcttca
    20641 ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc ttcgtcgatc
    20701 aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc ttgtttagag
    20761 gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac acccatgtca
    20821 gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg tttgattaca
    20881 ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc atcaaagtgg
    20941 aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata agcaatgttc
    21001 agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga gtaaccagct
    21061 gcatgtcctt tttggctttc atgatacagt tggttcaagg aagtagcgag ctttccactt
    21121 cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata ctcatttttt
    21181 ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc gatttctgag
    21241 tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt cgtggcaggc
    21301 tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc acgtaattca
    21361 tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg gatcttatta
    21421 cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt caatttttgc
    21481 agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg
    21541 tcaactagct tcccaccaaa ttctaaatat aatttgtcat agtggttcac tcgttccaaa
    21601 atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt tttcactagg
    21661 ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca actggaagag
    21721 cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga tagttattca
    21781 ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa
    21841 cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc ttgaagatcc
    21901 cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa ctcgtgatga
    21961 gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa taagataaag
    22021 gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa aaaaaggctg
    22081 taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt ataacaattt
    22141 tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag taagaggagg
    22201 caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa ctagaggaga
    22261 cgaatgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag
    22321 caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa gctgtccgcg
    22381 aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa gaattaaaaa
    22441 gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc catgatcgtt
    22501 ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg atgagctatt
    22561 acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca agtctgccag
    22621 aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa ccggagtcaa
    22681 cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct cagacagaaa
    22741 ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca
    22801 ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga
    22861 agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg cctgaaacaa
    22921 tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt cgtgcagcca
    22981 tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag ggattcggtc
    23041 aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat aatcaatcta
    23101 atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa aaatgaagaa
    23161 caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag taatcctctg
    23221 gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta
    23281 actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac tgaaaatccc
    23341 gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat tgaaacagcg
    23401 gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga ttttccacaa
    23461 ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac gatcggaaag
    23521 acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga tattggtctg
    23581 gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat caattatacg
    23641 ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga tcaacgatac
    23701 caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat caatgtccaa
    23761 gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt ctctgcaggg
    23821 cagatcacga tattcaatca caaattgaat caattattag cccatgcaag aacacttggt
    23881 tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa tcttcagagc
    23941 gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat gaaaaatcca
    24001 tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga taaaggacag
    24061 tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc atctggagac
    24121 tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc attaatggtg
    24181 agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt ctgttccaaa
    24241 ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt actaaatgga
    24301 cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa taaaatagca
    24361 cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca atttattccg
    24421 aatttccagc caatgactgc tattttttta tttatagtac tttacttaag cttaaaagat
    24481 gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg tgttggacca
    24541 tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc aatcgtctgc
    24601 ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt tctggcgggc
    24661 atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt gcctcaacct
    24721 tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg caatgttgtg
    24781 ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat
    24841 gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt tgacaagttg
    24901 cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa
    24961 agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa
    25021 aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag aagataacgg
    25081 cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct attggatgtt
    25141 tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga atgatggaca
    25201 atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta aagggcgaat
    25261 attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg aaaattttaa
    25321 aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag ttgctatcag
    25381 caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa gcatccacag
    25441 tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag ccaataaagg
    25501 acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca gtacaatcgt
    25561 cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt ttgaaaagtt
    25621 gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac tgttatttgg
    25681 tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac
    25741 tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa tactgattat
    25801 ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag gaacaatatt
    25861 gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga tcggtttctt
    25921 gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca tcggtgaaag
    25981 cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg agatcagtcg
    26041 gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg cagcaaaagc
    26101 agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa
    26161 tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag ataaatatga
    26221 cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat
    26281 ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg
    26341 ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg
    26401 gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc
    26461 ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat
    26521 tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga cgcaagcatc
    26581 atttgcaacg acattattga tggttttctt tttagaatta cgctatacat tattaggagc
    26641 aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc
    26701 actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc cttcctggaa
    26761 taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg gtagcaatat
    26821 gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc attttgcttt
    26881 gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac tgatctttac
    26941 aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa atactttcgg
    27001 attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga aggcattcaa
    27061 aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc cgctcttcaa
    27121 cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt ttagttgctt
    27181 gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt actcatcgaa
    27241 aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta tttgcggcgt
    27301 tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg aatatcaaaa
    27361 tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag
    27421 ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt ttattttatt
    27481 aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc atttgcaaca
    27541 gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg gcttatgatg
    27601 caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat tttttgctat
    27661 aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg aataaaaaat
    27721 tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt ttaggggtag
    27781 tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg cttgctacga
    27841 cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt acagtcggat
    27901 ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat aacgctgctt
    27961 gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc gcctctgctg
    28021 cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct aacgttaata
    28081 atgggatact agctatcaga agtttatgcc catctttgat tgctatccca attcttagtg
    28141 caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact tctttgatta
    28201 tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg cgcgtattga
    28261 ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc ttcggtgggt
    28321 tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc gaacttaaag
    28381 attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct gtctctatca
    28441 ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt caaatgatcg
    28501 accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca gatcagcaac
    28561 tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg atcctgatct
    28621 ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg aagaaaaaag
    28681 atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta ttgattcttc
    28741 ttccttcatt gactggaatg tctttattag ctggaccact atacacattg ttttttggct
    28801 atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg tttttctctt
    28861 tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt gcgatccgat
    28921 taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta ggattagttg
    28981 gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt gttcgcggct
    29041 atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc aattttttct
    29101 taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc
    29161 tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt gtggctgtag
    29221 gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt
    29281 tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg ttttttgctg
    29341 tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt tccaatttta
    29401 gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt ctttgaaacg
    29461 aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat ctgccggaac
    29521 gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg gatgggaaaa
    29581 tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta aaaaacagta
    29641 aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta tatgaggaat
    29701 tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg atttctggct
    29761 ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc attccttatg
    29821 actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta gacttaaatg
    29881 atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga gataatacct
    29941 acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt ttgacaaatg
    30001 gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt tctgaaggga
    30061 aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa
    30121 ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg atacatgcaa
    30181 ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat ccaaatgcag
    30241 aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa aaagccatcg
    30301 gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa aatattccaa
    30361 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
    30421 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
    30481 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
    30541 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
    30601 agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag ggctttcact
    30661 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
    30721 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
    30781 agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
    30841 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
    30901 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
    30961 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
    31021 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
    31081 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
    31141 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
    31201 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
    31261 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
    31321 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
    31381 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
    31441 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
    31501 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
    31561 gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc gactgtcatc
    31621 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
    31681 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
    31741 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
    31801 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
    31861 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
    31921 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
    31981 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
    32041 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
    32101 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
    32161 ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
    32221 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
    32281 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
    32341 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
    32401 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
    32461 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
    32521 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
    32581 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
    32641 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
    32701 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
    32761 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
    32821 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
    32881 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
    32941 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
    33001 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
    33061 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
    33121 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
    33181 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
    33241 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
    33301 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
    33361 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
    33421 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
    33481 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
    33541 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
    33601 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
    33661 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
    33721 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
    33781 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
    33841 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
    33901 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
    33961 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
    34021 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
    34081 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
    34141 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
    34201 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
    34261 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt gcattaagcg
    34321 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
    34381 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
    34441 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
    34501 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
    34561 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
    34621 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
    34681 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
    34741 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
    34801 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
    34861 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
    34921 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
    34981 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
    35041 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
    35101 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
    35161 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
    35221 cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt gtcaccgata
    35281 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
    35341 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
    35401 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
    35461 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
    35521 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
    35581 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
    35641 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
    35701 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
    35761 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
    35821 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
    35881 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
    35941 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
    36001 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
    36061 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
    36121 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
    36181 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
    36241 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
    36301 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
    36361 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
    36421 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
    36481 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
    36541 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
    36601 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
    36661 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
    36721 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
    36781 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
    36841 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
    36901 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
    36961 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
    37021 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
    37081 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
    37141 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
    37201 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
    37261 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
    37321 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
    37381 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
    37441 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
    37501 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
    37561 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
    37621 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
    37681 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
    37741 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
    37801 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
    37861 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
    37921 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
    37981 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
    38041 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
    38101 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
    38161 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
    38221 gttccattaa ataaagaata gg
//
LOCUS       DACEJR010000022        36937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEJR010000022 DACEJR010000000
VERSION     DACEJR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.022"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..521)
                     /locus_tag="IW651_001127"
     CDS             complement(93..521)
                     /locus_tag="IW651_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813869.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(549..1187)
                     /locus_tag="IW651_001128"
     CDS             complement(549..1187)
                     /locus_tag="IW651_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0813870.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1191..3227)
                     /locus_tag="IW651_001129"
     CDS             complement(1191..3227)
                     /locus_tag="IW651_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0813871.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3353..4018
                     /locus_tag="IW651_001130"
     CDS             3353..4018
                     /locus_tag="IW651_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813872.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5953..9102)
                     /locus_tag="IW651_001131"
     CDS             complement(5953..9102)
                     /locus_tag="IW651_001131"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0813873.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSVENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9115..10314)
                     /locus_tag="IW651_001132"
     CDS             complement(9115..10314)
                     /locus_tag="IW651_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0813874.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10304..11899)
                     /locus_tag="IW651_001133"
     CDS             complement(10304..11899)
                     /locus_tag="IW651_001133"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0813875.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12483..13190)
                     /locus_tag="IW651_001134"
     CDS             complement(12483..13190)
                     /locus_tag="IW651_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813876.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13255..14091)
                     /locus_tag="IW651_001135"
     CDS             complement(13255..14091)
                     /locus_tag="IW651_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0813877.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14109..15518)
                     /locus_tag="IW651_001136"
     CDS             complement(14109..15518)
                     /locus_tag="IW651_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0813878.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15532..17355)
                     /locus_tag="IW651_001137"
     CDS             complement(15532..17355)
                     /locus_tag="IW651_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0813879.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18198..18524)
                     /locus_tag="IW651_001138"
     CDS             complement(18198..18524)
                     /locus_tag="IW651_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813880.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18570..18932)
                     /locus_tag="IW651_001139"
     CDS             complement(18570..18932)
                     /locus_tag="IW651_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0813881.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18969..19184)
                     /locus_tag="IW651_001140"
     CDS             complement(18969..19184)
                     /locus_tag="IW651_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0813882.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19311..19646
                     /locus_tag="IW651_001141"
     CDS             19311..19646
                     /locus_tag="IW651_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0813883.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19722..21719)
                     /locus_tag="IW651_001142"
     CDS             complement(19722..21719)
                     /locus_tag="IW651_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813884.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21817..22092)
                     /locus_tag="IW651_001143"
     CDS             complement(21817..22092)
                     /locus_tag="IW651_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813885.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22324..23058
                     /locus_tag="IW651_001144"
     CDS             22324..23058
                     /locus_tag="IW651_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0813886.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23097..24239
                     /locus_tag="IW651_001145"
     CDS             23097..24239
                     /locus_tag="IW651_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0813887.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24334..25725)
                     /gene="sufB"
                     /locus_tag="IW651_001146"
     CDS             complement(24334..25725)
                     /gene="sufB"
                     /locus_tag="IW651_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0813888.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25730..26200)
                     /locus_tag="IW651_001147"
     CDS             complement(25730..26200)
                     /locus_tag="IW651_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0813889.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26187..27422)
                     /locus_tag="IW651_001148"
     CDS             complement(26187..27422)
                     /locus_tag="IW651_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0813890.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27419..28705)
                     /gene="sufD"
                     /locus_tag="IW651_001149"
     CDS             complement(27419..28705)
                     /gene="sufD"
                     /locus_tag="IW651_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0813891.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28718..29491)
                     /gene="sufC"
                     /locus_tag="IW651_001150"
     CDS             complement(28718..29491)
                     /gene="sufC"
                     /locus_tag="IW651_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0813892.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29688..30536)
                     /locus_tag="IW651_001151"
     CDS             complement(29688..30536)
                     /locus_tag="IW651_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0813893.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30560..31246)
                     /locus_tag="IW651_001152"
     CDS             complement(30560..31246)
                     /locus_tag="IW651_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0813894.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31239..32273)
                     /locus_tag="IW651_001153"
     CDS             complement(31239..32273)
                     /locus_tag="IW651_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0813895.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32668..33006)
                     /locus_tag="IW651_001154"
     CDS             complement(32668..33006)
                     /locus_tag="IW651_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0813896.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33008..33364)
                     /locus_tag="IW651_001155"
     CDS             complement(33008..33364)
                     /locus_tag="IW651_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0813897.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33370..34557)
                     /locus_tag="IW651_001156"
     CDS             complement(33370..34557)
                     /locus_tag="IW651_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0813898.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34748..35593)
                     /locus_tag="IW651_001157"
     CDS             complement(34748..35593)
                     /locus_tag="IW651_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0813899.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35827..36792
                     /locus_tag="IW651_001158"
     CDS             35827..36792
                     /locus_tag="IW651_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0813900.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVISTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10953 a         7584 c         6120 g        12280 t
ORIGIN      
        1 caactaaaaa tgaatatatt aaaactacca aaaataactt ttaacgctgt ttcattatat
       61 gttttttcta ttttatataa acttttggat acttaatacc aaccggttgg attgtaccaa
      121 catgagaatg tgtcagttct atttcctgtc gcacttgcat aggcccatac tccttttgtt
      181 gcatttgctg atcgaataga gtttccaact ttcgcttttg caccgtgacg attatagaca
      241 cgactatagt ggttagaatg acctgttttt ctcgtacaag ttgtatcact ataataaacc
      301 catcttccag agctatgatt aattgctgtt ctagaaattt cccggtctac tggtaaattt
      361 gacttaataa tattaccttt agcatcaaaa tgaatctctc ctggctttaa agatttgtaa
      421 tagatttcac cgtgaactga atttgcttcc acttcagatg acatagcgtt tcctccaaca
      481 aaacctatcc ctagtgcaca aataataact gcttttttca taaaacgaca tcctttcctt
      541 tttgattttt aaagcatgat cttcttgttt cctattttgg tcagatatgg atcatgcgtg
      601 actacgataa ctaacgtacc ttgggaagct tgttgcgata agatttcaac tactttatcc
      661 ttatttcttt tatccaatga cccagtcggt tcatcggcta aaatgatttt gctattcttc
      721 aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt gtagataggc
      781 gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc tgttcgctta
      841 tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc gattgtttca
      901 tattcaatca acccaaaatc ttgaaaaaga aaagatatgg tatttctttt tatcaagcgc
      961 ttctctctcc cagttaattg tttcgtctct tgatcgaaca aatagaattg tcctgaactg
     1021 aactgatcat tcaatcctaa tatattcaat aacgtacttt tacctgctcc actttctcca
     1081 acaacagtca caagatcccc agcatccgca gtgaaacgat ccaaatgaag aatcgttttg
     1141 tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg ttaacctcca
     1201 ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac tgaaagaata
     1261 aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct ttctataaga
     1321 aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa gaaatcttta
     1381 tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg acaattgtag
     1441 acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa agtgagtaaa
     1501 aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat ctcttcagtc
     1561 tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc aatcagctca
     1621 aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata gatttctccg
     1681 ctgagaatat ttctttccaa atacatactg tttttcaatg tcagtatttc aacaaccgga
     1741 taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt gttttttata
     1801 aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat ttgcgccagg
     1861 tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg gtgcgtttct
     1921 ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt cttaagataa
     1981 ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa gtaatctgat
     2041 acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg atctgctgct
     2101 gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt tactggataa
     2161 aatacatgat agtcctcacc agcgatttct tttggtggtt catgggaaac agctaactgt
     2221 gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag aagagaaaac
     2281 aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct taacgaagcg
     2341 attttttcaa ctaataaaag agaaatcaaa gagataccat atattccgct gaacaagaaa
     2401 acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat aaaaccacca
     2461 atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa acaccacaat
     2521 acttgatgta atgaaaagtc attattcaac aataaactga tttctttatt catcaataag
     2581 aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata cactcgataa
     2641 ttatatacgt ctgtatttga aataaccgaa tacgaatagc tattttcaaa gtcttcaaaa
     2701 atattagtgg cataatcagt gtgaaatgct tgattgaatc gatcagtgaa tagtgaaatg
     2761 acccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc aaaatctttt
     2821 attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata gggtaccttc
     2881 tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt tggctgtatc
     2941 ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt catataagaa
     3001 agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata ttcttcttgc
     3061 ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg gaaaaattca
     3121 tttgtccgat agagtacctg tttatttata ctaaccaagg caaaaccact catgataccg
     3181 gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac tctcccttac
     3241 ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat ctatcaagtg
     3301 tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga agatgaacac
     3361 cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag aattaggcga
     3421 aaaaattggt gtttccgata aaacgatttc tagttgggaa aattctcgta caatgccaga
     3481 ctttgaaatg ctgtcacttt tacatcatac tttaggtctg ccgattgatt ttccttctat
     3541 tgaatcagct accgaatcaa tagaaccaac attgccagat gaacctcttg ttaacaagaa
     3601 atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct ttctgctcgt
     3661 cttttgtttg gtctatcttt ttcttatcaa ttatcaatac aaaaatgaaa cgatcgatcg
     3721 attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag gatacgtgga
     3781 caattccatc gaacgttggg tatgtaatga tatctttggc catggtacat ggctgtcttt
     3841 tcacgctgga gaggtagaaa aagagaaaaa ccatgctgta atcaaacaca aaggaaaaca
     3901 cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc aagaaatcct
     3961 cagccaagac tatgacgacg ccttaaaaag aagccctcag gagccagata ggcattaagt
     4021 aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatccaa aaaacagggg
     4081 tacaaattaa ttgaaaaagc cttgagatga aagttcctaa ataaaaaagt tcagaatgat
     4141 aggtatttct ggcttatagt ataatattta cgccacagcc tcccaatgat tctttttgga
     4201 atcataatca aggatggaca ttcacaaaaa tattttgctc attcgatccg aatagattaa
     4261 cgaccaatct agtaagtaat atagattggt acgtagtcaa gctgcacata atttagatca
     4321 attttagttc ttttttatat ttctatgatc tgctcattta cttttatatg atggcttaca
     4381 gtaacataaa taaatatgat aatcgctggt agtttatcca agagtactca ataaaataca
     4441 aaatggattg ttcaagcgcc tatcataatg ataaaatgaa acaaataaac gatacaagga
     4501 ggaagagact ttgagtaatg gcagaacaat tttttcattg taaaaattgt tcagttgtta
     4561 atctatggga tttcggtagc atgggcattt ttttatagag gaatcacacc aacctggccg
     4621 ttttatctct tgttagtggg aatattagta ggaaattttg cccctcttca ctatcgtacg
     4681 aacttttttc caactgattc tggccttttt cttaaaacaa agacgcgaaa attagaaaac
     4741 attattgaaa tgacaattac ctgtataatc attttcttag ttatattcct aaacgtcttc
     4801 taataagaat ctcttaaaat tagcttgtta cagttagtta cctagtttat ataacacagt
     4861 tatcttatct tccttatcaa catcttttgc caactctttg tcttaaattc gaaacatata
     4921 agcagagcta cgaacaccaa gactgtttcg gctctgtttt ttgcatcaaa ttcactctta
     4981 tatagctgat aatctcggct ctttaaaaga aattattttc gcttcatttt attataaaaa
     5041 ggctaggaac cttgatgtta tcaggttcct agcctttatt taagtattac cccacagacc
     5101 cttccatata gatttttaaa ttacacacta gtttaaacta aaacaaacaa ggaaaaactt
     5161 accatttaat caaaaaatta gctcatatga atatattaga atgtaaatta gactggtcaa
     5221 aatctggtca aaaaagagga cgaatacagg tagtcctctt ttttgttatt ttgatattct
     5281 aaatcatttt ttattggatt attcccttgt tcgatataga cccaaattga tctatcaata
     5341 attatcaaac tgagtcatat agagacaaaa atttcaaaaa tgacctgtta aatggtcatc
     5401 gaatatcatt taaaatcaat acgacggagt cagacacgga gtcaaaacaa gcccgctgag
     5461 agtaaaaaat atacagtccc ataggtaaag tgccacagga ctgtatattt ttttatttga
     5521 gattacctga aatgattcca tttaattttt atctcatttt ttcctactag ttagtatcaa
     5581 atactctcct attttctcaa taaactattt tgggttacat ggtatctgaa acagctaaaa
     5641 ccttgtcatt atagcaaaaa atcactttat ctccgtttca tttcagatac atttcactta
     5701 agcggattcc aaaaaaacaa aagaaaaagg aacccctagt tagcctatgc tttttattgg
     5761 ttcctctcaa aacacggtca tataacaacg gggtggggta aatgaatgaa aaaggtggag
     5821 gaaacattat tcaatagctg tcttaaatac ttcgttataa aatgtgaaca aataaaagac
     5881 atctatccct attttctaca cttccacttg actaccatct ttgaatcgta caattttctt
     5941 aactcttctc gtttacctca attcattctc aagtggaatc aactcatcat ctaacatttt
     6001 acgccatgct cgcttcattt cttgtgggta tttaaatggc ttcgcttctg gatgtttggc
     6061 tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa ttggatcttc
     6121 acctggtaga tattgcagat tagaagcatg taattctttc tcagaaacaa accactcatt
     6181 ggaaaagaca tgaatcaaat catcactcgc ttgtgtaaaa tagcgacgtt taatgacaaa
     6241 gatatcttca ttactatcta aagtccccac atcaaatgca tttaaaattt gtcggtaaac
     6301 ttctttgact ccttctgttt tattcaattt agccaaagct ttttcaattt cttctcgtgc
     6361 atctgaacta ttcgcagcat aagttcctag taattgatca atataagttg aatcaatatc
     6421 atacaactta gtggtatttt cactataaaa ttcgatcgtt gaaaaatctt ctccttcttt
     6481 ttctggttct tcctctatta atgaaccctt cacggtctcg tacacgccaa tttgttcttc
     6541 caaaagttga atttgattgt tcaaagttgg agatttcacc atgtcatcat tgtaatcgtc
     6601 ataagtcact aacgattcat aagcacgatt caattcttga aatgctttga cgtactcgat
     6661 acggatttca agtggcgtat gttcatcaat ggattccatg gatgaaacct cttttaactt
     6721 accatatgct tgtttgaatc tttttttgga ttcctgataa cttggataaa tcaaacctga
     6781 ttcttctttt gcttttgaat acagttttgt cgcatcctcg acattcttct ccattgtcgc
     6841 tggcttacga aacgtcacaa tcaatccttt tgtcttatca ggatacgtac gattcgtacg
     6901 tgaaaaagct tggatcaatc ctgcatactc tagattacga tccacaaata gggtttgaat
     6961 cgtaggggcg tcaaaacctg ttaatagacg atccactaca atcgctaaat cgatttgttt
     7021 cccaaattgt ttgaattccg ctctttttcg tgccaaacga ttattgatgt ctccgttata
     7081 acgctcaatg tctgctaatg accaggaagt atcataatac tgattatatt cctcaataat
     7141 tttttgcatt tcttcttgtt gggcacttga atctcggcta ttttcttcta atgaataggt
     7201 aatagcgatt cgtggaaaat caggatcctc catcgttcgt ccttcacgaa tgagctgatt
     7261 gggaaactct tttgttaacc aatcaggctc tttcgtcatc tctttgatgg cccgataata
     7321 ctttttggcc atgtcaatgg aactcgtggt taagattgca gatttcgttg gcctgccatt
     7381 tcgaaaatca aattttgtat aggcattatc tggacggaac attttatgaa tgactttttg
     7441 gatatgttca tctgcttcaa aaacagctgg gtccaaataa ctttcttttt tgacaggttc
     7501 catttgatca atcatacgat tgatctgttc tgatgaatac tctgcatatg tttctacttc
     7561 ttttaatttc cgatagattt tattctctac tgaagtcggc tccacggtgt tttcatgctc
     7621 tacttgaaat cctaagacag agccatcttc tagcgcattc ttaatggtat aggtatgcaa
     7681 gacctctcca tactgatcat gggttgtacg tgccaattgc cctttggctt gtttccgatt
     7741 ctcaggaaaa atcggtgtac ctgtaaagcc aaaccaagtg gatttcggaa acattttttt
     7801 gatttcttcc atattttcag cacttaacgc tcgatgacac tcatctacta caaacacgat
     7861 atgttggccc attaattttt ggaagcgatt ggttcctttt ttctcttctt gtttctttgc
     7921 atatttcaac gccgcatcta gtttttgacg ggttgtaatg attacaacat ttgcattggc
     7981 atctgccaac agggtttcac ttaattcttt ggcacttccc gttccaacga tcaagctatt
     8041 cgctttggcg ttcccggaag aaatcccagt attaaattcc gaagcaaatt ttgtaaactc
     8101 tgtggtggtt tgattatcta aatctttgcg gtccactaac atgatcgttc gatcgatccc
     8161 cgattttcgt gctaaaagct tagtagatac aaaactagtc aacgtctttc ctgaaccagt
     8221 cgcatgccac acataccctg attgatgttt atttgcggca ataaacaagg cttgaatcgc
     8281 atgtacttga taaggatgta acaccatgag ggttttatta tcttgatctt cactgacgat
     8341 tgtataatcc gcaatcaaac gatgagcatc cgggatattt aacacttgtt tacaaaactc
     8401 atataagttt tctacttttt cattgtcttt cgttcgccaa ctaaataaaa acttagggtg
     8461 caagtctttc ggtaaggcat ttgcaaaata ccgagtggtt tgttcattgg aaaccacaaa
     8521 caactgtaag gtcgaaaaga tattgttccg aaacatacct tcttctgcat attttttgat
     8581 ttggttaaat gcctgataaa caccatcttt tgcagttact tgtttcagct cgatttggac
     8641 aatcggtaat ccattgatta ataaggtgac gtcaaaacgt cgatcccgtg cttctatgtt
     8701 acttccacgt tttgcaattt gatggaccac ttcataagta gagattcctc caccaatgtc
     8761 ttgattggaa taaaggataa gtgagacaga acccaattgc gaatcttccc gttcaatcgt
     8821 gatccgagcc atcccatttt caccttttaa ccatttcgca gcatcaaatg gggttttcgt
     8881 tcgcagcagt aattccgttt tgatcgtttc aaattcttta tcggtcaaag gaaaatcatt
     8941 cagttctgct tgattatttg agataatttt ttggcgtaaa ttgacccaaa ggtcttcttc
     9001 tgattttaga tccggacgat acgtccactg attatgccct tctccaagta cacgaatcaa
     9061 ctgttcttcg acttctgctt cctcacggtg actaattttc attcttcttc cctcctagac
     9121 aaacatcttt tgtaagaagc tttttttcgt ttcttttagt aaatctaact tacgttgatg
     9181 aagagcgatc gtatcatcta gctgtttgaa gaaagtaccg attttttgtt gttcatcctt
     9241 tacaggaacc ttaacactga tattctcaat atcagaactg ttataagaag gcaatgcccc
     9301 tacttgggag tatcttggca aatttatagt ttcaaacaat attttaccaa aatagatatc
     9361 ccaagtatta ttaaataaat aagccatggt attgttatct attaagaaag ggcttgtgac
     9421 caaacgtttt ctattaagca ttattgctgc gccaacttta gcaaatataa tcgctggcac
     9481 atcttttatt ggcttccatt ttttacgctc aatttgctca ttagatacat aatgatttgc
     9541 atttctcatt tcatactcat taccaatgtt attcatgtcg gaaactttaa aaaatggagt
     9601 accttcagaa ccaccttgct cggcatcagg aaatccaatt cccgattgag ttttacctaa
     9661 ttctcccaac ttacgctctt cccaatcttc cgtaaaacca ggaaaccgga tttctggcac
     9721 ttttgctccg ttttcaggga acattttttg taagaatcct tttttcgttt ccttcagcaa
     9781 atctaactta cgttgatgaa gagcgatagt gtcgtcgagt tttttgaaga atatgccgat
     9841 tttttgttgt tcttttaaat cattgggaat atagaatact gtatctgaaa cttttttcca
     9901 gtctgaacga ggcatttttg ttccagatgt atcatttgca gccttttgat atctatcagc
     9961 ttgaataaga gaataaataa agtccgagtc gctcttctta gctctaaaaa cccagaaatc
    10021 cccaatcgct acgcctgtaa aagttgcctt gagccaattt tttaaatatg gacgtaactt
    10081 tccatataga atatcatttg gtaaaaattc aatccctttt ctattatcaa atttctggct
    10141 cacatcttta ttcagtctac cttctcctgc cacgatatct tcaaattcaa cttttggaag
    10201 tctactggag tttgtacttt tattaagtct aacaactaaa tcaattaact tacgctcttc
    10261 ccaatcttcc gtaaatcctg ggaaacggat ttctggtcgt gtatcattgc tcatgtttga
    10321 acacctccag cgcaccttgt aaccacattt cgttttcagg tgttgtttgc agggaagaga
    10381 tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc ttgatgtcat
    10441 ttccgacatg aaccatatca attggttctt cttcttcgaa agtatctaca tagcgtggga
    10501 tatttaagtt atagtcattt tctttgattt catcaaaggt tgccaaatga gcgtatttct
    10561 cgacatcttt tctttcagcg taggtttcga ggattttttg aatgttttct tccgatagtt
    10621 tattttgatt tttcccttta acaaattcac gactagcatc aatgaataat acatctcgag
    10681 tttgacgatt cttcttcaac acaatcaccg tcgttggaat cgatgtccca aagaacaagt
    10741 tcgcaggcat accgatcaca gcataaatac tgccatcttc tagtaatttt tgacggatga
    10801 ccccttctgc agcgccacgg aataaaactc cgtgtggtaa gacgatggcc atggtacctg
    10861 tttccttcaa atgatagaac ccatgaagaa gaaacgcaaa atctgctttg gattttggcg
    10921 ctaatttccc ataacgatta aaccgagaat catctaagaa agtcgtatct gctgaccaat
    10981 tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca tctgttggcc
    11041 agtccttatt caacgtatcc ccattgcgga gattcatttc ttcggcgtcg acaccatgta
    11101 aaatcaagtt catttttgct agattgtatg tcgttgtatt tagttcttgc ccatgatatt
    11161 taacgttatc tgggtgtgtt aagtagttac gtacgtttaa catcaatgaa cctgaaccca
    11221 tcgttggatc aaaaacacta aagaatcgtc gttccttttg atcgagcgtt acgatttgcg
    11281 ccatcatatc agaaaccata tgaggtgtgt aaaattctcc ggctttcttc cctgcttctg
    11341 aagcaaattg actgatcaga aactcatacg catccccgat cacgtcccca tcgtgttcta
    11401 aaacatctac atcattcaat ttcttgatga cttcggtaat cgtcacattt cgttgttgct
    11461 catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca ttaaattgat
    11521 tgtaggtaga tgccaattgc acaaaggctt tgtttagatc gttcaattgg aacgttgctt
    11581 gttttgcttg atccgctaag acgttgaaaa gatactctgg tgcaattgca tatcccaaaa
    11641 tatcaacgat tgtggcaatc agatcttctt ttgattcttc atctgaaagt aattcacgat
    11701 acaacattgt ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa
    11761 ccacttgttc caacaatcga tcggataaat atttatagaa aatcagtccc aataaataat
    11821 ttttatattc tgacgcatcc attttactac ggagattatc tgccgcacta aataattttt
    11881 gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt gctatatacc
    11941 ctcttatttt agcatacccc cttttataaa aagcaaaaaa aaaagaaaga aacttattct
    12001 tgctaataaa ctacgcgaaa ggagtctaac gttgtttgat ataacttaga ctgatcgtta
    12061 acttattttt tatatttcta catagttatc tttagaacca tttcagatac atatgaaaca
    12121 attacatatg attttttcat aaaaaaacag tactgtctag tagtataaaa tatacaaatt
    12181 tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa
    12241 cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa
    12301 tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc
    12361 atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact
    12421 tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct
    12481 ttttacccat tttcaagtaa ccgaagtgga atgtctttac caacttgctc tgtcactgca
    12541 ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc
    12601 tctgtatcag tatttatcgt gattccttta tcttcattta caagcttcca agtaggtggt
    12661 atagctgtgt atgtttcgat gaatgaatca attctttcat ctatcgtctt cccagtatct
    12721 acttctgtct cattactctt agatgaatcg attctttcat ccatcgtctg ctccgtaact
    12781 gcttctgtct cattattctc accggaaact ctcttcacac ccaaacttat atattgtcca
    12841 tctttagtgt gcatagttaa aattcccacg cttttactaa taaattcact ataaccagtt
    12901 ctttcaatag agacttcaat gtatttcaca ctatcatact caaaagaaaa atagacatgt
    12961 aaaagcattt tgttgtttac atctacataa taatcctttg actctacttg agaccttcct
    13021 atacggaata cagctttact gtctgatatc tctataccag catccttatt attaatctgc
    13081 caatctccaa gtagctcttc tggcaagcta gtagtttctt gtgaacactt taccacttca
    13141 ctggtagagg tcgaaacatt cacctccgca ctgttagaag aattttccaa ggaatcagga
    13201 gccttagcac aaccaacaag tctttatata ttgtgcagcc gatattttct tcattcagta
    13261 cacccttgaa atatgcagtg ttagataaag cagttcgtcc tgatttgctg aggcacccat
    13321 ctcttcttga acgtactcct tgattttcaa ggccgcccga tactctttcg gaaaacgctg
    13381 cttcactgaa gccagcaaaa aatcatcctt ctccggcaac tcttccttct taatcattcg
    13441 ggcaataaaa aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac
    13501 ctttttttcg ggaaacagat atcctagcat agccaagata tcctttagtg ctcttaccga
    13561 gagcatggtt tcttccatat ttgcttgttc actttgggca tttacaatgt gaaacgcaat
    13621 gtttccagcc tcctcttctg gcaactgaac attcattttt ttgttgatca attggatagc
    13681 atagcagccg atctcaaatt cttttgggta atacttttga atttcaatca gcaggcgatt
    13741 ttgaaaaaca atgccttttt gactgcgttc aatggcatgc ttcaagtgat caaccaaggt
    13801 aaaaaataga cttttattca gcttaaagtc aaacttggcg ttggcataat cgataattcg
    13861 cttgacgcaa ataattagat catccggagc agcctcaaac agtttaatgt aatccttttt
    13921 tagagcatca gaaagtgaat ctaatttaaa tactttttgg attttgtcag ccggaaccaa
    13981 atcgcctttc tttagttgaa atccaatccc tttccccatc aaaattactt ctttgccttg
    14041 cttatctaag caaagaatta aactgttgtt taacgtttta attactctca ttagattcac
    14101 cgttctctct aagctaagcc atttcttgcg attacttttt gataccaaaa gaagctttct
    14161 ttttttattc gttttaattc tttaagattc tcatcggttc ggtcaacata tataaaacca
    14221 taacgcttat tgaagccttg atgggtacta acaacatcaa tcgctgacca aggtaaataa
    14281 ccaaaaacat ctacaccatc tgttaaagct gcttgaatag ccagaatatg tttggctaga
    14341 taatcaattc gatcctggtc attaattcgt ctatcttcct caactatgtc gggtgcccca
    14401 tagccatttt cagtaattaa aataggtaaa tcatagcgat cgtacaattt cctcaatgtt
    14461 aatcgtagtc cagtagggtc aactacccag ccatatggcg ttttatctac ataaggattt
    14521 tcagctgccc ggtagacacc ttcttctccc aacatgatct gttgatcccc cgctctagcg
    14581 gaaacatctg atccgtcatt tttgctggca gcgatggtgg cagttgaata ataattaatc
    14641 gcaataaaat cgggtttacc ggaggataaa attgcctgat cctctgcctc aattttagga
    14701 tataatccac gatcattcaa atagttttca aacaaataat tgtattttcc ccgaactgct
    14761 acatctaaga aagaccagcc tctcaatgtt tcccaattat gggctgcaat tgcatcttct
    14821 ggactggagg tagcttgata catagccgtt aaattgattg ctggaccgat ttttccttta
    14881 agattcaact gatgatacaa gtgaattact ctagcttgag ctaataaaac atggtgatta
    14941 atttgaaaga gttctttttt tgaaggcagc tctccacttg gtggcaaacc aatcgctcca
    15001 ggatgaagga tcatcgtgtt ttgctcatta attgtcagcc aatagttcac cttgtcacca
    15061 tactcagtaa gcaatacctg tacatagtcc acaaaataat cgatagttgc tcgatttaac
    15121 caaccgccct gctcctctag aaaataaggt aaatcgaaat ggtacatggt gacaatcggt
    15181 tcgattccct gattcaggca ttcatcaatt acttgatgat aaaaagaaat tcccttttga
    15241 ttggttttat tattttcaat atccataatt cgcgtccaag cgatggaaaa gcgaaaagct
    15301 tttaagccta attctttaaa cagctgtata tcttctttat agtgatggta aaaatccgag
    15361 gcaacttcaa aattagctac cccttctgta tgctgatact tgtcaataat agacggttgt
    15421 ttcccatcct cctgataggc accctctgcc tggtaggcac taatggaggc accccataaa
    15481 aacttctctg gaaatgctga aagctgtttg tattgcacaa aaatcctctc cttaactaat
    15541 ttgcatcaat ggttcgtgtt cctttaagct tccgcttgct tcactaattc ctgcgtaatt
    15601 ttcactgttg gtcactacca caatcactgt atcatcgtag ccagcgtttc taatggcagc
    15661 gaaatcggcg ttgattaata attgtcctgc tgacacctga tctcctgctt ttacaaatac
    15721 ttcaaagggt tcccctttta aattcacggt gtcgatcccc acatggatca ataattcaat
    15781 accttcttta gaacgtagac caatcgcatg tttagattct aaaatagtaa tgacttctcc
    15841 ctcaacaggt gaataaatgc tgttttcttc tggatgaatg gctacgccct ttcccaatag
    15901 tcccttagaa aagacagcat cgttcacttc agaaatagga cacacggttc cttttaccgg
    15961 attttcaaca atgactgctt cggctattgc cgtttgctct gattcatcag aagtatcgtc
    16021 aaatccaatt agataagcaa tgataaaggt tgcaacaaag gcgattacac acgtaataat
    16081 tgcgtgaaca atattcattg gattttctcc aataaaggct ggtaaggctg ctaagccagg
    16141 ggaaacaaag gcatatctga ctaaaccgct caagccagca tacaaaccag caattgcacc
    16201 accaatcatg gaagcaacta atggtttttt cagcttcaag gttactccat agagtgaagg
    16261 ctcagtaatt cccattaaag cagtgatccc agtggacaca gctagctgct tcaactcttt
    16321 gtttttagtt ttcgcggcta cagcaaaggt agcacctgat tgagcaatat tagaagccag
    16381 cattcctggc ccattgatca tttcagaacc cgtactgctc aattgcatag tagcgattgg
    16441 cgtcattgcc caagctgtac ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa
    16501 tggaattaac caagctgctt tgccgttaat ccattcagcc cccattgcta taagatcgtt
    16561 cagcaacgtt ccaaacggtc caatgacaac caacgctaaa ggccccataa ttaaaaatac
    16621 cagcatcggc ttagtaaaga atttaataaa cgaaggcgaa attcgatccg caaagggctc
    16681 tacataggac atgatccaaa cagtaaagat aatcggtaaa actgatcccg cataatctgc
    16741 agctaatacc ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat
    16801 atttggatga agcaaaatgc cagcaacagt aatcgccaaa attgggttga catcaaactt
    16861 tatggctgcc ccataggcta atagtactgg cataaaataa aaggctgcat cagcaatagt
    16921 attcaaaatg atgtaagttt gactactgtc tgatagtaca ccagtcaaag tcaagacggc
    16981 caaaatggct tttaccatcc cggcacctgt tatagctgga atcatcggtg taaaggtggt
    17041 agaaatgaca ctaatcaatc ttgaaataat actttcttga ttgtctgaag aggggccctt
    17101 tttaattgaa gttcccactt ccttttgaat ttccttgaag gctgtttgga cttcattgcc
    17161 aatcaccact tggtattgtc caccgctatc caccacacta attacaccaa gtgttttttc
    17221 taaggctttc gtatcggctt tccttttatc tttgagttca aaacgcagtc tggtggcaca
    17281 atgggttaag ctgaccacat tttcttttcc cccaacctgt tgcaatacat ttcttcctac
    17341 ttctctgtaa tccattattc tctctcctta aagcagtaaa aaagaccaag tcaaaccacg
    17401 ccccaatcaa cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc
    17461 caataacgtt attttgctgt aatccaataa tagcacatta tgatagcgca atcaacaggg
    17521 aatttaattt tttatctttt cgtttgaccg tatagggtaa tataacagtt agatcacatc
    17581 aactagggta tatctgcgta tacttactgc taaaataagt atatctaata ttaactatac
    17641 cagtaaaacc attcctacat aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa
    17701 tgcatataac ggatcacaca acttcaacaa ttctgctctt gggaaaaatt gatgaatttg
    17761 gtgagtaaag gatggaataa acagcattcc aacaatatag gcaacaatag tgatacttgt
    17821 ttttttggta aaaatcagta agcaatagcc aatttcaatc attccgagat ataaaggtat
    17881 caatagaatg atttgggcta gtattcttaa aaattaacta gcaaatgttt aaactgattt
    17941 atttctaatc tacatgtagt aagtatttca attccaccaa ttatcaataa aaataccaaa
    18001 catatccata ataatttttt ctttttcatt tcttttatat tgttataaca gtgaataaca
    18061 taaaaattcc cgacaatcat tgtaatattt ttttgtcacc tgtattagta tttaatttat
    18121 aggaaactat aaaaactccc tagtgaattt acacctttgg gagttcctgt ttggacggta
    18181 tgcataatac atttaaatca atttaacatt aacacctcag catatggtat ctccacatat
    18241 atacggtctt tctttggata gtactcaata tttatgttga tagcacttcc attgtctaat
    18301 atagctttag cccggtatgt gttatctcca ttttcatgag taatcgtcac atcatcacat
    18361 gatcgattta agttgtactg ttcttgtaaa atctgtgtta ccaatggttt tgatgacttc
    18421 tctaatccag atgtacctgt catttttgaa aaaataatac ctataatagc caagacaaca
    18481 atacctataa tgacaaactt cttgttcata tttactttat tcaacttttt atctcctatc
    18541 tccaaaataa tttgttctta tccctcttat cagtacattg acatccctaa aagtacacca
    18601 aagattgctc cagctacccc tgctatcatc aaaatcgtac ccattttctc atctttttct
    18661 cgatataagt agcccataat aacagcaaca gcaccaaata caacaggaat aaatgctaac
    18721 gatactacca tagaaatcca accaataatc aaatacagct tccctgtctc ttgtccatgt
    18781 acgatttgtt ccttcatttc attaattgtc tgctcagtac ttttctttac tgtgtctgtt
    18841 ccggttgtct catctgtttc ttgtagtgct ccacaaaact ggcaatactt actttcatct
    18901 gttatttctt tgccacattt tctacaatac atgtcttctc ctcctaaaat cattcaattg
    18961 cttattagtt aactgtttac tttattaaat acatcagaaa acagctcatt cattggtaca
    19021 cttaatatct cagaaagcat cttcatttga tagggtttag gaatcgttct ccctgtttgc
    19081 cacgaactta cacattgctg tgtaacattc attttttttg ctaactccgt ttgagaaagt
    19141 cctttttctt ttctgtatcg gctgagatta cttacttttt tcatgattct cccctcctgt
    19201 tttattatat acaacaatat gatgtatttc aactcataaa atataaaaaa cttcaaatac
    19261 cattacaaat acaacaactt gttgtactat actgagatgg gggtgtatta atgaaacaga
    19321 aacaatatgc aaaggtcatt agagaaaagc gaaaagctct cgggtggaca caaaaagaat
    19381 tagctgaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
    19441 agcctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
    19501 ttctagacaa tgttgtcgta gattatgagg aagagttttt ggctctctat cgttctctta
    19561 gcactgaaga tgcagttcga acaattgact atatgaagct attaaaaagg caagaaaatg
    19621 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
    19681 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tacaattggg
    19741 tcttttctct tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgatacaga
    19801 ggaactatcc cagctcctga taatatcaat tcttttattg tttcatctat ccctgcccct
    19861 ttcgctaaat atattaaaaa actactactt gctttcacat tgtatagttt cttaatatcc
    19921 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttttattaaa
    19981 gcatttttca cgctgttatc cccaacatta cgtaggcgtt cacaaacagt gttaataagt
    20041 tctttctctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
    20101 tcatctaaaa tcaattgttt ttccttgctg agttctgatc gggaattttg aggaaatact
    20161 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
    20221 gtaaaatgaa ttttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
    20281 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgattaaa gtttcctaac
    20341 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
    20401 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
    20461 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
    20521 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
    20581 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
    20641 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
    20701 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
    20761 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
    20821 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
    20881 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
    20941 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
    21001 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
    21061 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
    21121 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
    21181 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
    21241 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
    21301 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
    21361 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
    21421 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
    21481 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
    21541 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
    21601 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
    21661 ttctctaata atttttcttg atctacataa attggaatat taatcattga tgtttccatt
    21721 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
    21781 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
    21841 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
    21901 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
    21961 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
    22021 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
    22081 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
    22141 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
    22201 atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaacagct
    22261 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
    22321 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
    22381 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
    22441 aacggtcaat taattgacaa aaaaactatc tacgaatggg agaagggaac ctatttacca
    22501 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
    22561 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
    22621 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
    22681 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
    22741 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
    22801 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
    22861 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
    22921 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
    22981 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
    23041 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttaatgg
    23101 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
    23161 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
    23221 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
    23281 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
    23341 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
    23401 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
    23461 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
    23521 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
    23581 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
    23641 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
    23701 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
    23761 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
    23821 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
    23881 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
    23941 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
    24001 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
    24061 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
    24121 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
    24181 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
    24241 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
    24301 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccatctcata
    24361 actgatcaat cgattgagtt cgactgcata ttccattgga agttctttgg tgaatggttc
    24421 aacaaagccc atgacgatca tttcagttgc ttcagattcg gtcaggcctc ggctcatcag
    24481 atagtagagt tgttcttctg agatttttga gactttggct tcgtgttcta aagagacttg
    24541 gctgttgtgg atctcattga atgggatcgt atcggatttc gaccattcgt ccatgatgat
    24601 cgtgtcacat tcgatatgag agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc
    24661 tcgatagttt acttctccgc cgtcttttga aattgattta gagacgatag agctagatgt
    24721 atttggtgca ttgtggatca tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa
    24781 ggcgatggag agcatggtgc ctcgtgcacc ttttccatct aagtaaacac ttgggtattt
    24841 catcgttgtt tttgcaccta ggtttccgtc gatccattca acggttgcgc cttcgtatgc
    24901 ttttgctcgt ttggttacta agttgtacac attgtctgac cagttttgga tcgttgtgta
    24961 acggcaatac gcatcttttc ttgtaaagat ttctacgatg gcggcatgca ggctgttact
    25021 tgaataagta ggtgccgtac aaccttcgac atagtggatg cttgctcctt cgtcaacaat
    25081 gatcagcgta cgttcgaatt gccccgtgtt ttccgcattg atccggaagt acgtttgtaa
    25141 tggaacatcg actttgacgc cttttggcac gtaaataaag gtcccacctg accatacggc
    25201 tgaattcagc gctgctaatt tgttgtcggt tggcggtaca agttttgaaa agtactcttt
    25261 gaataattca gggtattctt tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt
    25321 ttcgaattca tctttcatat tatggtacac gacttctgat tcatattgtg cggaagcacc
    25381 ggctagataa gctcgttcag cttctggtat accaatcttt tcaaatgtat ctttgatttt
    25441 ttctggtacg tcttcccagt cacgagcggg tttgtcactt ggtttttggt aatatttgat
    25501 ggcatcgaaa tcgatgtctg aaaggtcggg gccccatgtt tgcattggca ttttgttaaa
    25561 tgtttctaga gatttcagac ggaattcaag catccattct ggttcgcctt tgattcttga
    25621 catctcacgg atcacttctt ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc
    25681 atcgtgaaac ccgaatttat attcttctaa ttctggtacg cccatgttct cactcctctt
    25741 ccttgtggta atgatggaaa gctgcggtac catcgttttc cattgctttt cctagtgctt
    25801 tccaggcaag tgtcgcacat ttgatccgag cgggaaattt agctacacca ccaagcatag
    25861 cagcatcgcc taattggtcg ggatctttga cttcttttcc ttggacaagc tgtagaaagt
    25921 ctacagttaa atgttctgct tcttctattg attttccgat gacggcgtct gtcatcatcg
    25981 aggcacttgc cgtactgatg gagcagccac tgccgtcaaa ggcgatattt ttgatgattc
    26041 cgtcttttac atcgacttgc aattcgatca catctccaca tgtggggttg ttcaattcga
    26101 tcttgcttgt cgagtcagtc aagcttccac gatgatgcgg atgggaagaa tgatccaaga
    26161 tcacttgacg gtataaattg tctaatttag atagtcccat gttggaaaaa ctcctttgtt
    26221 gccatgattg cttcaactaa acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc
    26281 gcgcgagcag tcgctgatac ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct
    26341 gctctgacgg ctaccccttc catgtctaaa gctgtcgcga catcatgggg atgtaaccca
    26401 tccaaattga atgcaacgac tcctgtccga aaattcgggt cttgtggacc ataaacagtc
    26461 aatccatcaa ttgcttgtaa ttttggcaac acataagcgg ctagttcttc ttcatatcga
    26521 tggatattgt ccataccgag tttttgcaaa taatcgattg catggcctaa tgcgatagcg
    26581 ccggcgatat ttggtgtccc tgcttcgaat ttccaaggta gttctttcca tgtactgtcg
    26641 tataattcga caaaatcgat catttcgcca ccaaattcaa caggttccat ttcttctaac
    26701 cattttcgtt taccatataa aacaccaata cctgttggtc cgcacatttt gtgtccgctg
    26761 aaagcaaaga aatcacagtc tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga
    26821 gcaccatcta ctacgatgac cccgccgttt tcatggacaa gttcagctaa ttcttgaaca
    26881 ggattgaaga cacctaagac attggataca tgagcaatgg agacgatttt ggttttgtcc
    26941 gtaatctttt tcgctgcatc ctccatatcc aggaagcctt cttctgttag ttcgatatat
    27001 ttcaagacag cacctgttcg ttttgccaac tgctgccacg ggataacatt agaatgatgt
    27061 tccatgtaag agagcactat ctcgtctcct gctttgatat agcggtcgcc gaaggttctg
    27121 gctacccaat tcaagcttgt cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt
    27181 gcatggatga acgcccgtac tttttctctt gcttgttcat aatcatgggt cgcgcgttct
    27241 gctaacgtat ggacaccgcg atgaacattg gcgttatcgt gttcgtaata gtacgctaat
    27301 gcctggagta cttgttttgg cttttgggtc gtcgctgcat tatccagata aactaaaggt
    27361 tcatcattga cgtcttgaaa taagatagga aaatcattgc gtagcttttt agcatcgatc
    27421 atccattcaa cttcccttcg attacttcga ctaattcttt ccgaacttgt tctacaggga
    27481 ttgctgttaa gacagaaccg aggaatcctc gaatcactaa gcgctctgct tcttctttat
    27541 gcaatcctct actcatcaga tagtacattt cttctggatc aactcgtcct acactggcag
    27601 catgtccagc agttacttcg ttttcatcga tcaaaagaat aggattcgca tcaccacgtg
    27661 ctttatcaga aagcatcaag acacgacttt cttgttgggc atctgcacct tttgcccctt
    27721 tcaagatgtg gccgatacca ttaaaggtca atgttccttt ttctcggatc actccgtgtt
    27781 gcaaaatatg cccgatcgta tgaggggctt tattggttac tcgtgtatcg attccttgga
    27841 tttgtctgcc cgagctgatt gccacgattt tgacttctgc gtgtgcacct tctcctgcta
    27901 aatctgagtc aaagtcagcg atcacatgac cgtcattcat gacaccaatt gcccagtcga
    27961 cagaagcatc tcgtaaaatg tgcccacggc gattcatata agaagtaata ttttctccta
    28021 attgatctac ggctgaatat ttgatttttg aaccagcttt agcgatgact tccacgatga
    28081 tattacctga tgattttgct acttgttctt tggttgtctg gaaacgttcc agataagaaa
    28141 attcactatg ttcatctgcc acgatcaaca catgcttgaa gaagtgctca tcggatgcgc
    28201 catcttgaat gaataaggac tcgagtggtt catcgatcac gacattttta ggcacataca
    28261 ggaaaatacc gctgttcata aaagctacat ggagtgcagt caattggtct tcgttcatgt
    28321 ttactgcttt tttcatataa tattcttgga ctagttctgg atattcgatc attgcagtga
    28381 acaagtcggt aaaaatgact cctttttctg ctaattccac aggcagttgt tcaaaaatcg
    28441 tccatgaacc ttgttggaca agcacaggat tgtctttcat tgcatcaaat gccggaatcg
    28501 tgcctgtttc actgatggtt tgtgtttctt tcacatcaaa taacgaccag cgatggaatt
    28561 tcacccgatc gatatgcggc aatggcagtt catctgcttt cgctagtgcg tcttgacgga
    28621 aggttctcat ccaatcaggt tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat
    28681 aatcgagcca gtttttttct ttcattcttg ctccccctta cgcttcttct tctttatagt
    28741 cgattcctaa ttctttactg ataccagcgt agccttctgc ttccagacgt ttagctaggt
    28801 cagcattccc tgtcatgact actcggcctt ccatcatgat gtgaacgaca tcaggtgtga
    28861 tatagttcaa taatcgttga tagtgggtga tgatcaatgc accgaaattg tctccgcgca
    28921 tttcatttac tcctttagca acgactttca aagcatcgat atccagacct gaatcgattt
    28981 cgtctaaaat tgcaaatgta ggttctaaca tcaacaattg caggatctca ttccgtttct
    29041 tttctccgcc agaaaaacct tcgttcagat aacgttcagc catttcttct ggcatattta
    29101 gtaaagccat tttttcatct aactttttca aaaatgccat aacagaaatt ttgtcgtttt
    29161 catcacgttt cgcattgatc gctgcacgca tgaattccgc attcgtgata cctggtatct
    29221 cacttggata ttgcatagct aaaaataagc caagtcgcgc acgttcatct acttccaagt
    29281 ctaaaacatt tttcccgtca aacaaaattt cgccttctgt tacttcatag tttggattgc
    29341 ccatgatagc ggcagataag gtagattttc ctgtgccgtt aggtcccatg atggcatgga
    29401 tttctccggt tttcattgtt aggtttacgc ctttaaggat ttctttttcc tcgatcgaca
    29461 catgtagatt ttttatttct aagacagaca tgcattatac ccccaaattc tctttcgttc
    29521 tcactcattt tagactaatt gagaatgaag cgcaatgacc ttccttacta aaatcaaata
    29581 tcttttattt attattaaac aattttgatt tctaaattag aataggtata aaaaagatga
    29641 gaaataagcc attacagact tatttctcat cttttttgtt ccatacctta ttttgcatca
    29701 gcctttactg gtttaacaga gccgccccat ttgtctgtga tccattcttg gacttctttg
    29761 cggtgaagca cttcaatcag ttttttgatt ttgtcactgt cttcatcacc tttacgagtc
    29821 gcaataatat tcacgtaagg agagttgtcc ttttcaagca atacggcatc ttctaatgga
    29881 ttcaaccctg cgccgtaagc gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt
    29941 tggtacgtag aagctaaaag tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg
    30001 ttttcttcaa tatcatcgaa tgttgctgtt tccagatcta ctccatcttt gactttgact
    30061 aagccagcat cttgtagaat tgtgatgatg cgtccccagt ctgattcaga atttgaagta
    30121 atgattgttg caccatcttt taattcggaa acatctttga cttttttaga gtacaagccc
    30181 attggttcga tgtggacagc accggcattg acaaagtcat atcctttttc tttgatttct
    30241 ttgttcaaat aaggaatatg ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat
    30301 tggttcggta atacatagtc atcgaatttg acgatttcaa gatcgattcc ttcatctttc
    30361 aataatggtt tcacatgttc caaaatctct gcgtgcggtg tcggtgaagc gccgactttc
    30421 aatgtcgtgt cgtctgcttt atttgctgaa tctttgctgt cgctgttgtt accgcatgct
    30481 gctaatccta cagtcagcaa aagcgctgct gcaaatccaa aaattttctt tttcatatcc
    30541 tacatccccc tatatcctat tatcttttgt ctgttttctt agttaagaaa tcaccgattc
    30601 cttgaacgat aaatacgatt agtaaaataa ttaaagtcgc aacgaatgtc actgttagat
    30661 taccacgctg gtagccttcc tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc
    30721 cggccatcgc agtaaatccg atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg
    30781 cgggaatact ttcaggtatc aagaccttcc agatgatttc ccagtaactt gctcccattg
    30841 catctgctgc ttccagtaca ccagggctga cttcacgaaa agcaatctct actagacggg
    30901 cataaaatgg tgctgcggat aaaacaagag ctggaatggc tgcttgcgca cctagcatag
    30961 tgcctacgat tgctttagta aatggaatga tcaaaaccat caggatcata aatggcgttg
    31021 accggaaaat attactgatg atcgatacga ttccataaat gattcgagca tacggtttgt
    31081 ttttgcgtcc aatagagtaa aggatcaatc ccaaaactaa tccgatgatc gtcactaaga
    31141 ccattgagat taatgtcata tatagtgtat caaggatagc ctgattcatc gtttccgggt
    31201 tgatttgttt aaaatctaga tatgtttctg aaaagctttt atccacgatg gatcacctca
    31261 attcctactc cttgttcttt gatgaacact tctgcttgat ctaattcttt ggtttcacca
    31321 gtcatcaaga cagtcaatga accaaaggaa ccttctttca cttgtttgat ctttccatac
    31381 acaacgttga catcgatgtt atagcgtcga atcgcttggg aaatgactgg ctcgttggca
    31441 tttctttcgc taaaggtcaa agttgccaaa cggccgcttg gattttcttt gatgagttca
    31501 gctaatagtt ctgtcgaatc agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt
    31561 acttcttttt tcggttgtct gaagacagtc aatacgtctc cttcttctac gactttcccc
    31621 aactccatca cagcgacttt gttgcagatt ttgcggatca cattcatttc atgtgtgatc
    31681 aagacgatgg tcaaattcaa tttcttattg atcgctaaca ataagtctaa tacttcttct
    31741 gttgtttgcg ggtccaatgc gctagtagcc tcatcacaaa gcaggatctc aggatcattt
    31801 gccaatgctc gtgcgatacc gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat
    31861 gcttgtccgc gtccttctaa cccgactaaa cgcaacagtt cttctgcccg ttctttacgt
    31921 ttgctctttg gtacgccagc taattccagc ggcagctgga tattttcaag tacagttctt
    31981 gaccataaca ggttgaaatg ctggaagatc ataccgattt tctttcggaa tgtacgtaat
    32041 tctttgtttt ttaattttgt aatatcttga tcgtttacga tcacttcgcc gctcgtggga
    32101 agctcaagtc cattcaggag acggactaat gtactttttc ctgcgccaga atagccgacg
    32161 attccataaa tatccccttt ttcaacgttt agtgaaacgt cattgacggc ttggatgttt
    32221 ccgtttttcc cattgaatgt ctttttgaca ttcgttagtt ggattaatga catgccattc
    32281 acatctttct cttatgattt ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa
    32341 ggacgaaagc ttgaacttcc gtgttaccac cttgatttac cgttacttcg caataacagc
    32401 ctcttccagt acttggactt agcttctttc cgtatactgt cgcgctgtaa cgggcactcc
    32461 cgtagtaagt ttgcttacgc tcgcttactc tgctctaaga ccatcttcca tctcattttc
    32521 gttacccatt ttcagcaaac agggctctct tcaaacgtcc gtgacatgta ctcttctttt
    32581 caaagctttc tcgtttcata taggattgct tatgattata gcaactggaa gaatcagtgt
    32641 caactttttt caagtgattt tctgatttta taattcgtca aattcttttg gatcaacatc
    32701 tttgaaactt aagatccacg cattcatttc atctgaatca ttcaagatat tgatatcttc
    32761 accgatttta tcattaacag aagaaactgt acccgttagc gggctggcaa attcattgac
    32821 cgctttttcc gcttcaactt cgatcagagg ttcatctttt tttagcgatt gtcctgcttt
    32881 tggcagattg gcaaacgtga tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac
    32941 acagtattct tttccattaa ataagatcca aaggttgtcc tttttcttca aatgcttgcc
    33001 tgccatttta tatcagttct ccttcataaa ttttttcatt gaatccaatt gcaacaagct
    33061 ggtcctctcg taagatcagt ggtcgcttga tcagcatacc atctgtcgat aagacttggc
    33121 tcgcttttga agccgtaaac tcgcctactt gatctttcag tccaagttcc cgatatttca
    33181 tcccgcttgt gttgaacagg cgacggattg gtacttgact cgtttctagc caagtctcaa
    33241 tcgttgaggc cttcggtact tctttgatca tatcaacggt ttgataacta acgccatgct
    33301 ggtctaacca cgctttggct tttttgcatg ttgaacattt cgggtaccag taaaaggtgt
    33361 acaattttct tacctccttg aagatcttac aggtgcttca ttttggtatc tcatcgataa
    33421 tatcaacccg ataccgatca tgttccctaa aatcgaggat cctccctgac taatgaaagg
    33481 caatgggatc cctgttaaag gaagtaatcc gatatttgct ccaatattct caaagacatg
    33541 gaacagcagc atcatgatca aaccgcttgc gatataggcg taaaattcat tattcgtatc
    33601 gaaacacacc cgaatcatac gatagattaa aataaaatac aagaaaatga cgaatgcacc
    33661 accgataaag ccgaagtttt caccgataac ggtaaaaatc atatcggact cacgaacagg
    33721 cacatagata ttgcttacgt tgaatccttt accaaacaag ccaccggtac caattgccaa
    33781 aagtccttgg gccggctgat aactcattcc tgacgtatca tggaaagggt ccagccatga
    33841 atcaatacgt gcaaactgat aagatttgaa tccaacttta taaagcaagt ctcttccgcc
    33901 ttcagtcgta accaagaaga tcgttcctgc tccaacgact actgccaaga ttactacagg
    33961 tgcgatgatg cgccaagaga ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa
    34021 gacaagcatc gttccaaagt catcttgggc catgatcaat gctaaaaccg gtattgtcac
    34081 gatcaagagc ttcccaatca gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt
    34141 attatgttta gttacgatca gtgccatcat caagatgtac gcaatcttca tcaactctgc
    34201 tggctggaaa gtaaacgcac cgaatcgaaa ccagttttta gaccctgttg aagcagctaa
    34261 attctgatca tagaatttga gcaagagtcc cattaccact aaccccaaac cataaaggta
    34321 aggagtcagt ttccagatcc atttggaatt caaatgcatg atgacaacga ttgctgccgc
    34381 tccaactaaa taccagacaa cttgcatccc tacgcctttc aagactgctg gattgttcgg
    34441 atcatggcta agtgctacat ataatgagag cagaccaatc aaacataaca gaaagactgg
    34501 taaaatcacg ccataatcaa tacgattatc gttcgaagct tttaattttt tttccatgtt
    34561 tcatcccttt tcttaaaaac catactgctg attattatag cggtccagtt agataatgga
    34621 aagtctttcc atagacattt gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa
    34681 atgcagtaca aaagcctact gcttatcttt agtgtatcat agaacttttt ttgatacatg
    34741 caattatcta ccagctgaga ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc
    34801 gaaggatctc ttcggcaatc tctagcagct gatcagaaac cagattggtt cgttggatga
    34861 aataaaaata tcgatggaaa ttcaacggag tcattggcac attttctgga acagagcgct
    34921 tagacaaaat cgagcgaccg attccttcct tcagcaacgc aacaattaca tcgttacttt
    34981 tcacgatcat cttcttatct tggatattca tctcttcaaa ataacgctgc gtgtaatgaa
    35041 aaacaccaga gtttgcttct cttacaagcc acaatttgct ttttgggtcc ccagcgatga
    35101 ctagctggtc ttccatcaaa gagtatcttt gtacgccgga agtctctaat ggcttttcga
    35161 taaaaccaaa atcggattca tgtttcgcta cttgttctaa tgattcatga gaattcgtga
    35221 tttcgatctc aaaatgcact ttaggaaacc ttttgatcag atgtttcatc aactgaggca
    35281 aaagatagac agcaaaggtg tgggaagcaa cgatccgaca tacttcttct tcattttgtt
    35341 cattgagcaa tttttgctga gtttcttccc attcttctaa caaaaccagt gtccgttcat
    35401 ataataagtc ggcttgctta gttggaagca tctcttttct tccgttacgt tgaaaaagat
    35461 cgatattcaa ttcccgctcc aactgtttga tttgattcga gactgctggt tgagataaaa
    35521 acagtacttc tgctgctctg gaaaaatttc ttgtttcata tactgctcga aaagtcgtta
    35581 ataacttaaa catggcacca cttccataac tttttatgat aattactata aaaactattt
    35641 gatttaaata tacaaaaaac catctataat agaaatgtac aacaaaacag ttgctgatcc
    35701 aactagtaac ttagatgaag acaatcagct gtctgcttgc tcgtaatcat acattcctat
    35761 tcattttaca atgtattcgt tcatcttggt actaaaatct attaaataaa aaggagaata
    35821 ttatatatga agaacttttt gaaaattatt ccgatcccga tctgcgggtt gatccttggg
    35881 ttagcttccc ttggaaatct gtttaaagac tatcatcatg ttgctttagg caatctaact
    35941 ggcggtatct ctatgattct tatgatattc atttttgtta aattgtttat cttattcgaa
    36001 cacacgaaac aaacgttgca tgatccaatc atcgcctccg tttcaccaac ttttacgatg
    36061 tcactcatgg ttatttctac ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa
    36121 ttcatttggc tgtgcgctgt tatcttccaa gtgatccttg tcttttattt caattatcac
    36181 catgttatca aagctgatct ttcaattgaa gcaatttatc caagctggtt tatcatttac
    36241 gttggatttg gggtcatcac tgtaactgcc gggaacttct tcccacttat tggaaaaatt
    36301 ttcttttgga tttcattgat ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc
    36361 tttttcgtaa gaaatatggc tcatccgaca cgtccgctga ttacgatcgt tgcagcacct
    36421 ggttctcttt gcctaacagg ttatcttaaa aactttgctg aacctaattt ttatcttgtc
    36481 gtagcattgt tcgctttatc tcaagtattg tatttcgttg tgctgtctat gcttccacgt
    36541 ttgttcaagc ttcgttttta tccaagctat gcagccttta cattcccgct tgttatctct
    36601 gcaacagcta tatttactac tgttcattac ttcaacagtt taggtatcta tagtagcgta
    36661 ttggatttct taaaagttgc ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt
    36721 gctcattacg tacgtttctt aattaaagaa catagcaaac atggaaaatt tgtttctaaa
    36781 aaaatcgctt aataatcgat tcaataggtt gtgaaaaagc tgttctgcat caagtaataa
    36841 gaacggccaa acaaaaacag ctgttcatgt ttttgtactg gttgggctta ttaccgcaga
    36901 tgcaagcttt tgaacaccgt ttaccaagag gtcgtaa
//
LOCUS       DACEJR010000023        34592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEJR010000023 DACEJR010000000
VERSION     DACEJR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.023"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(41..889)
                     /locus_tag="IW651_001159"
     CDS             complement(41..889)
                     /locus_tag="IW651_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0813901.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1113..3053)
                     /gene="thrS"
                     /locus_tag="IW651_001160"
     CDS             complement(1113..3053)
                     /gene="thrS"
                     /locus_tag="IW651_001160"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0813902.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3390..3569)
                     /locus_tag="IW651_001161"
     CDS             complement(3390..3569)
                     /locus_tag="IW651_001161"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0813903.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3616..5439)
                     /gene="cadA"
                     /locus_tag="IW651_001162"
     CDS             complement(3616..5439)
                     /gene="cadA"
                     /locus_tag="IW651_001162"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0813904.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5717..7996
                     /locus_tag="IW651_001163"
     CDS             5717..7996
                     /locus_tag="IW651_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0813905.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8089..8736)
                     /locus_tag="IW651_001164"
     CDS             complement(8089..8736)
                     /locus_tag="IW651_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813906.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8915..9634)
                     /locus_tag="IW651_001165"
     CDS             complement(8915..9634)
                     /locus_tag="IW651_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0813907.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9765..10916)
                     /locus_tag="IW651_001166"
     CDS             complement(9765..10916)
                     /locus_tag="IW651_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0813908.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11133..11873)
                     /locus_tag="IW651_001167"
     CDS             complement(11133..11873)
                     /locus_tag="IW651_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0813909.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11877..12200)
                     /gene="rsfS"
                     /locus_tag="IW651_001168"
     CDS             complement(11877..12200)
                     /gene="rsfS"
                     /locus_tag="IW651_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0813910.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12257..12850)
                     /locus_tag="IW651_001169"
     CDS             complement(12257..12850)
                     /locus_tag="IW651_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0813911.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12834..13481)
                     /locus_tag="IW651_001170"
     CDS             complement(12834..13481)
                     /locus_tag="IW651_001170"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0813912.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13494..13805)
                     /gene="yhbY"
                     /locus_tag="IW651_001171"
     CDS             complement(13494..13805)
                     /gene="yhbY"
                     /locus_tag="IW651_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0813913.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13821..14930)
                     /gene="yqeH"
                     /locus_tag="IW651_001172"
     CDS             complement(13821..14930)
                     /gene="yqeH"
                     /locus_tag="IW651_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0813914.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14927..15454)
                     /locus_tag="IW651_001173"
     CDS             complement(14927..15454)
                     /locus_tag="IW651_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0813915.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15634..16419)
                     /locus_tag="IW651_001174"
     CDS             complement(15634..16419)
                     /locus_tag="IW651_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0813916.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16412..17281)
                     /locus_tag="IW651_001175"
     CDS             complement(16412..17281)
                     /locus_tag="IW651_001175"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0813917.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17282..18661)
                     /locus_tag="IW651_001176"
     CDS             complement(17282..18661)
                     /locus_tag="IW651_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0813918.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18669..19088)
                     /gene="fabZ"
                     /locus_tag="IW651_001177"
     CDS             complement(18669..19088)
                     /gene="fabZ"
                     /locus_tag="IW651_001177"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0813919.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19113..19589)
                     /gene="accB"
                     /locus_tag="IW651_001178"
     CDS             complement(19113..19589)
                     /gene="accB"
                     /locus_tag="IW651_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0813920.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19592..20827)
                     /gene="fabF"
                     /locus_tag="IW651_001179"
     CDS             complement(19592..20827)
                     /gene="fabF"
                     /locus_tag="IW651_001179"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0813921.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20846..21583)
                     /gene="fabG"
                     /locus_tag="IW651_001180"
     CDS             complement(20846..21583)
                     /gene="fabG"
                     /locus_tag="IW651_001180"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0813922.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21595..22512)
                     /gene="fabD"
                     /locus_tag="IW651_001181"
     CDS             complement(21595..22512)
                     /gene="fabD"
                     /locus_tag="IW651_001181"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0813923.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22619..22843)
                     /locus_tag="IW651_001182"
     CDS             complement(22619..22843)
                     /locus_tag="IW651_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0813924.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22896..23858)
                     /locus_tag="IW651_001183"
     CDS             complement(22896..23858)
                     /locus_tag="IW651_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0813925.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23868..24308)
                     /locus_tag="IW651_001184"
     CDS             complement(23868..24308)
                     /locus_tag="IW651_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0813926.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24614..25579
                     /locus_tag="IW651_001185"
     CDS             24614..25579
                     /locus_tag="IW651_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0813927.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25636..25866)
                     /locus_tag="IW651_001186"
     CDS             complement(25636..25866)
                     /locus_tag="IW651_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0813928.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25952..26839)
                     /locus_tag="IW651_001187"
     CDS             complement(25952..26839)
                     /locus_tag="IW651_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0813929.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26928..28151)
                     /locus_tag="IW651_001188"
     CDS             complement(26928..28151)
                     /locus_tag="IW651_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0813930.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28168..29247)
                     /locus_tag="IW651_001189"
     CDS             complement(28168..29247)
                     /locus_tag="IW651_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0813931.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29378..31105)
                     /gene="ptsP"
                     /locus_tag="IW651_001190"
     CDS             complement(29378..31105)
                     /gene="ptsP"
                     /locus_tag="IW651_001190"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0813932.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31105..31371)
                     /locus_tag="IW651_001191"
     CDS             complement(31105..31371)
                     /locus_tag="IW651_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0813933.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31850..34084
                     /locus_tag="IW651_001192"
     CDS             31850..34084
                     /locus_tag="IW651_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0813934.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34232..34552
                     /locus_tag="IW651_001193"
     CDS             34232..34552
                     /locus_tag="IW651_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0813935.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9771 a         7483 c         6036 g        11302 t
ORIGIN      
        1 ttgttattct aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat
       61 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
      121 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      181 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      241 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      301 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      361 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      421 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      481 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      541 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      601 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      661 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      721 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      781 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      841 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      901 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      961 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
     1021 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1081 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1141 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1201 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1261 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1321 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1381 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1441 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1501 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1561 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1621 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1681 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1741 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1801 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1861 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1921 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1981 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     2041 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2101 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2161 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2221 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2281 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2341 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2401 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2461 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2521 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2581 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2641 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2701 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2761 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2821 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2881 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2941 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     3001 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3061 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3121 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3181 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3241 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3301 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3361 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3421 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3481 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3541 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3601 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3661 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3721 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3781 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3841 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3901 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3961 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     4021 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4081 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4141 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4201 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4261 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4321 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4381 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4441 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4501 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4561 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4621 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4681 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4741 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4801 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4861 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4921 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4981 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     5041 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5101 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5161 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5221 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5281 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5341 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5401 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5461 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5521 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5581 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5641 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5701 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5761 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5821 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5881 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5941 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     6001 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6061 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6121 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6181 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6241 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6301 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6361 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6421 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6481 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6541 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6601 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6661 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6721 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6781 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6841 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6901 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6961 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     7021 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7081 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7141 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7201 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7261 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7321 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7381 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7441 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7501 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7561 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7621 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7681 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7741 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7801 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7861 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7921 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7981 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     8041 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8101 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8161 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8221 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8281 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8341 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8401 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8461 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8521 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8581 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8641 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8701 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8761 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8821 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8881 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8941 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     9001 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9061 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9121 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9181 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9241 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9301 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9361 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9421 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9481 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9541 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9601 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9661 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9721 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9781 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9841 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9901 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9961 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
    10021 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10081 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10141 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10201 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10261 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10321 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10381 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10441 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10501 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10561 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10621 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10681 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10741 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10801 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10861 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10921 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10981 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    11041 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11101 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11161 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11221 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11281 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11341 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11401 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11461 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11521 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11581 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11641 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11701 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11761 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11821 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11881 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11941 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    12001 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12061 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12121 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12181 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12241 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12301 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12361 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12421 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12481 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12541 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12601 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12661 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12721 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12781 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12841 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12901 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12961 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    13021 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13081 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13141 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13201 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13261 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13321 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13381 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13441 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13501 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13561 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13621 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13681 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13741 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13801 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13861 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13921 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13981 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    14041 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14101 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14161 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14221 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14281 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14341 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14401 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14461 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14521 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14581 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14641 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14701 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14761 gagacatctt ggatctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14821 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14881 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14941 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    15001 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15061 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15121 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15181 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15241 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15301 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15361 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15421 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15481 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15541 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15601 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15661 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15721 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15781 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15841 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15901 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15961 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    16021 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    16081 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16141 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16201 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16261 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16321 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16381 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16441 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16501 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16561 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16621 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16681 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16741 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16801 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16861 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16921 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16981 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    17041 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    17101 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17161 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17221 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17281 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17341 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17401 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17461 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17521 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17581 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17641 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17701 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17761 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17821 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17881 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17941 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    18001 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    18061 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18121 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18181 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18241 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18301 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18361 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18421 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18481 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18541 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18601 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18661 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18721 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18781 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18841 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18901 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18961 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    19021 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    19081 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19141 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19201 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19261 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19321 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19381 attatttctg gagcagttgt tgcttgttca gatatgacat tgactggctc cgaaactggc
    19441 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19501 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19561 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19621 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    19681 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19741 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19801 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19861 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19921 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19981 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    20041 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    20101 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20161 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20221 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20281 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20341 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20401 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20461 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20521 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20581 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20641 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20701 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20761 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20821 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20881 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20941 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    21001 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    21061 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21121 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21181 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21241 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21301 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21361 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21421 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21481 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21541 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21601 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21661 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21721 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21781 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21841 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21901 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21961 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    22021 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    22081 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22141 tccagctggg gtagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22201 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22261 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22321 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22381 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22441 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22501 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22561 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22621 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22681 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22741 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22801 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22861 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22921 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22981 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    23041 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    23101 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23161 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23221 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23281 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23341 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23401 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23461 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23521 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23581 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23641 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23701 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23761 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23821 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23881 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23941 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    24001 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    24061 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24121 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24181 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24241 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24301 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24361 atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt
    24421 ttttaagatt ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta
    24481 gaaaaatcat ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata
    24541 gaattcccta aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt
    24601 aaaaggagat cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta
    24661 tcttatgaaa gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa
    24721 tagagtaagc agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat
    24781 tcatcatata acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct
    24841 cgagtgtcaa ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag
    24901 taattataat tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc
    24961 tcgaacatac gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga
    25021 agaaacttat caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct
    25081 tcctgaccta caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca
    25141 aggccgaaat aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca
    25201 gttgaatttc gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat
    25261 tagacggatc caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc
    25321 attagcagca gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga
    25381 aacaatgatt acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca
    25441 tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag
    25501 ttaccgcaaa aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc
    25561 tgaaaagcaa ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt
    25621 caattatcct aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt
    25681 atcaaattcg tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt
    25741 atcataagtt actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc
    25801 attatctgct gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg
    25861 tgtcatcgga ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact
    25921 tgcattgatt ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt
    25981 caggccacca aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca
    26041 actcttcata tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg
    26101 cttcttctaa tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt
    26161 aatcttcttt taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac
    26221 ctgaaagcgt ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca
    26281 tttccgtcat tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct
    26341 gacctttccc agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca
    26401 gtacttgatc ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg
    26461 ctcctaagtc accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg
    26521 tttttgcaat tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc
    26581 ctgagatatc tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg
    26641 ggtaacctac cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg
    26701 cccagatagc tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag
    26761 ctacttcgtg tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc
    26821 cgataaaacc aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc
    26881 tagtatacgc tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat
    26941 gaatttaatt ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct
    27001 tcttcatgat aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta
    27061 gatgaaatat cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa
    27121 atataatcaa taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa
    27181 ggatgatcga acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct
    27241 gccatcgctt ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa
    27301 tctgccgctt tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca
    27361 aattcttcta actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt
    27421 cctacgataa ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc
    27481 aaggcttgga tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt
    27541 tcctgaagac ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt
    27601 ttgaattttt caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag
    27661 ctgcgtaatg tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca
    27721 aataatctag acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa
    27781 tgaaggtagt cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta
    27841 gctaccatct tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt
    27901 tctagttctt tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc
    27961 ttgaaagaaa taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca
    28021 ttaggatcgg tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt
    28081 aatgttttga tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag
    28141 ccgattttca tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt
    28201 gctagagact ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt
    28261 attgatactt cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg
    28321 attggtaatc tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt
    28381 cttgctatct tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt
    28441 gcttgctaaa gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa
    28501 tagatctgca cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag
    28561 gacattttca ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat
    28621 gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa
    28681 atcaatgatt cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg
    28741 agacagtttg aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc
    28801 taagtcgatc ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa
    28861 ctgttttgaa taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag
    28921 atattttttt acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc
    28981 ctctgtagaa tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat
    29041 attatgacta ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc
    29101 tagtgtataa gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc
    29161 gattccagat tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg
    29221 tcccccttca cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta
    29281 tttttgtcta ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta
    29341 gctcttttac aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa
    29401 gttcaacgac ttcttccatc atatcgcatt ctttcaatgc acgatcagca agttcagcca
    29461 ttttcgctgt gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca
    29521 ttgagaactc atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca
    29581 tttctccaca catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga
    29641 tcaaacgtaa aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac
    29701 ggtctgctgc cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt
    29761 cttttgcaaa cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt
    29821 ggatcgtatc agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga
    29881 tcgcttttgc tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat
    29941 taccatgaac agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt
    30001 gttcagataa actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta
    30061 attgcagata tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt
    30121 ttccttccat accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga
    30181 agtctggaga atccatatac aggaattcag tacggtacag accaactgct tctccaccat
    30241 tattatggac accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct
    30301 tgccgtcagc tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg
    30361 cgtaatctgc acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta
    30421 catcgccttc gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt
    30481 cttttgtccc aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag
    30541 tacgtccacc aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat
    30601 cacttggagt caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg
    30661 gtaaagtaac gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag
    30721 cacgctcttg catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg
    30781 ttacttcttt tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt
    30841 gacctaccat ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg
    30901 cttcacctaa gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag
    30961 ctaatgcagc atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga
    31021 atgttaaatc cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg
    31081 caatcccttt tagcatttca accattattc agataagcct tctttcttca ttgtttcaac
    31141 gattgctgcc attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc
    31201 ttgacctaca cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt
    31261 atactctaag tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg
    31321 acgagcgtgg atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg
    31381 tctcctttaa gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat
    31441 cctaacaaat gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca
    31501 gttttcattc aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt
    31561 cttcacattt tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga
    31621 cgtttatcat tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc
    31681 ctttttattt ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct
    31741 tttctacctc ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga
    31801 gatgtaaaat atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca
    31861 aaactgcggc aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac
    31921 acaactcgat tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct
    31981 gactaatatg gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc
    32041 catgtctcgc caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg
    32101 cggaaacaac aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg
    32161 agaatacgga atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg
    32221 acgcgatgac gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc
    32281 tgtcttgatt ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa
    32341 aatcgtagat ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt
    32401 cgtatcttta gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt
    32461 gattgaagag atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat
    32521 cgtcggtgct ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc
    32581 tttagctcga ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat
    32641 cgaaaaagac gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt
    32701 cgaagaaacg atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt
    32761 aaaatatact gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga
    32821 tcgcttcttg ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt
    32881 gacgatccaa atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca
    32941 aaaagtttta gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat
    33001 caataaactt caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac
    33061 cgaaaaagat atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa
    33121 agaaaaagaa cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg
    33181 acaagataac gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa
    33241 caaacagaat cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac
    33301 agagcttgcg aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt
    33361 cgatatgtct gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg
    33421 ttatgtcgga tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag
    33481 tcttgtcttg ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca
    33541 aatcttagat gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac
    33601 gatcatcatt atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg
    33661 tgcggcacgc gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc
    33721 agaatttctg aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt
    33781 gatgacaatc gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact
    33841 tcacatcgaa gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc
    33901 tatgggtgct cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga
    33961 attttatctt gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa
    34021 aatcatcgtt acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga
    34081 ataaatccag ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt
    34141 ttcagaaaag tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag
    34201 caattaaaaa tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca
    34261 ttcccgtctt gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac
    34321 agccggaaat accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat
    34381 caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga
    34441 taaaatcaaa gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct
    34501 tgttgaaatc tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt
    34561 tgaacataat aattaaagag cgtgggacaa aa
//
LOCUS       DACEJR010000024        33706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEJR010000024 DACEJR010000000
VERSION     DACEJR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.024"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW651_001194"
     CDS             4..816
                     /locus_tag="IW651_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0813936.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW651_001195"
     CDS             813..1613
                     /locus_tag="IW651_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0813937.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW651_001196"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW651_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0813938.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW651_001197"
     CDS             3110..4819
                     /locus_tag="IW651_001197"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0813939.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IW651_001198"
     CDS             5031..9383
                     /locus_tag="IW651_001198"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0813940.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IW651_001199"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IW651_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0813941.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IW651_001200"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IW651_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0813942.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IW651_001201"
     CDS             11221..11514
                     /locus_tag="IW651_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0813943.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IW651_001202"
     CDS             11511..11822
                     /locus_tag="IW651_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0813944.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IW651_001203"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IW651_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0813945.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IW651_001204"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IW651_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0813946.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IW651_001205"
     CDS             complement(14623..14844)
                     /locus_tag="IW651_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0813947.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IW651_001206"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IW651_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IW651_001207"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IW651_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0813948.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IW651_001208"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IW651_001208"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0813949.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IW651_001209"
     CDS             20242..21027
                     /locus_tag="IW651_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0813950.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IW651_001210"
     CDS             21040..21834
                     /locus_tag="IW651_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0813951.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IW651_001211"
     CDS             complement(22014..22466)
                     /locus_tag="IW651_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0813952.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IW651_001212"
     CDS             complement(22931..23299)
                     /locus_tag="IW651_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0813953.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IW651_001213"
     CDS             23449..23931
                     /locus_tag="IW651_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0813954.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IW651_001214"
     CDS             23968..24978
                     /locus_tag="IW651_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0813955.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IW651_001215"
     CDS             complement(25093..25806)
                     /locus_tag="IW651_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813956.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IW651_001216"
     CDS             complement(25996..26586)
                     /locus_tag="IW651_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0813957.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IW651_001217"
     CDS             27070..27297
                     /locus_tag="IW651_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0813958.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IW651_001218"
     CDS             27313..28479
                     /locus_tag="IW651_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0813959.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IW651_001219"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IW651_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0813960.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IW651_001220"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IW651_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0813961.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IW651_001221"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IW651_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0813962.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW651_001222"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW651_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0813963.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11454 a         5812 c         7275 g         9165 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaa
//
LOCUS       DACEJR010000025        33509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEJR010000025 DACEJR010000000
VERSION     DACEJR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.025"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            553..885
                     /locus_tag="IW651_001223"
     CDS             553..885
                     /locus_tag="IW651_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0813964.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1476..1805
                     /locus_tag="IW651_001224"
     CDS             1476..1805
                     /locus_tag="IW651_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813965.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            1792..2133
                     /locus_tag="IW651_001225"
     CDS             1792..2133
                     /locus_tag="IW651_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0813966.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2247..2486
                     /locus_tag="IW651_001226"
     CDS             2247..2486
                     /locus_tag="IW651_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813967.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2553..2792
                     /locus_tag="IW651_001227"
     CDS             2553..2792
                     /locus_tag="IW651_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813968.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            2919..3608
                     /locus_tag="IW651_001228"
     CDS             2919..3608
                     /locus_tag="IW651_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0813969.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            3614..4780
                     /locus_tag="IW651_001229"
     CDS             3614..4780
                     /locus_tag="IW651_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0813970.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            4862..5533
                     /locus_tag="IW651_001230"
     CDS             4862..5533
                     /locus_tag="IW651_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0813971.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5586..7562
                     /locus_tag="IW651_001231"
     CDS             5586..7562
                     /locus_tag="IW651_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0813972.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            7575..8327
                     /locus_tag="IW651_001232"
     CDS             7575..8327
                     /locus_tag="IW651_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0813973.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            8343..9104
                     /locus_tag="IW651_001233"
     CDS             8343..9104
                     /locus_tag="IW651_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813974.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            9117..9377
                     /locus_tag="IW651_001234"
     CDS             9117..9377
                     /locus_tag="IW651_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813975.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            9374..11464
                     /locus_tag="IW651_001235"
     CDS             9374..11464
                     /locus_tag="IW651_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0813976.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(11500..13557)
                     /locus_tag="IW651_001236"
     CDS             complement(11500..13557)
                     /locus_tag="IW651_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0813977.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(13702..13893)
                     /locus_tag="IW651_001237"
     CDS             complement(13702..13893)
                     /locus_tag="IW651_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813978.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            14081..14536
                     /locus_tag="IW651_001238"
     CDS             14081..14536
                     /locus_tag="IW651_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813979.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            14533..14775
                     /locus_tag="IW651_001239"
     CDS             14533..14775
                     /locus_tag="IW651_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813980.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14793..15095
                     /locus_tag="IW651_001240"
     CDS             14793..15095
                     /locus_tag="IW651_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813981.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            15164..17218
                     /locus_tag="IW651_001241"
     CDS             15164..17218
                     /locus_tag="IW651_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813982.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            17218..19830
                     /locus_tag="IW651_001242"
     CDS             17218..19830
                     /locus_tag="IW651_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0813983.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            19827..22205
                     /locus_tag="IW651_001243"
     CDS             19827..22205
                     /locus_tag="IW651_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813984.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            22266..22808
                     /locus_tag="IW651_001244"
     CDS             22266..22808
                     /locus_tag="IW651_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813985.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            22886..23218
                     /locus_tag="IW651_001245"
     CDS             22886..23218
                     /locus_tag="IW651_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813986.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            23219..23812
                     /locus_tag="IW651_001246"
     CDS             23219..23812
                     /locus_tag="IW651_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813987.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            23834..25858
                     /locus_tag="IW651_001247"
     CDS             23834..25858
                     /locus_tag="IW651_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0813988.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            25858..27057
                     /locus_tag="IW651_001248"
     CDS             25858..27057
                     /locus_tag="IW651_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0813989.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            27073..27717
                     /locus_tag="IW651_001249"
     CDS             27073..27717
                     /locus_tag="IW651_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813990.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            27728..28534
                     /locus_tag="IW651_001250"
     CDS             27728..28534
                     /locus_tag="IW651_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813991.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            28557..28786
                     /locus_tag="IW651_001251"
                     /pseudo
     CDS             28557..28786
                     /locus_tag="IW651_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28945..29880
                     /locus_tag="IW651_001252"
     CDS             28945..29880
                     /locus_tag="IW651_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ0813992.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            29924..30193
                     /locus_tag="IW651_001253"
     CDS             29924..30193
                     /locus_tag="IW651_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813993.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            30469..30744
                     /locus_tag="IW651_001254"
     CDS             30469..30744
                     /locus_tag="IW651_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813994.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            30786..31208
                     /locus_tag="IW651_001255"
     CDS             30786..31208
                     /locus_tag="IW651_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813995.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            31228..32820
                     /locus_tag="IW651_001256"
     CDS             31228..32820
                     /locus_tag="IW651_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813996.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            32841..33176
                     /locus_tag="IW651_001257"
     CDS             32841..33176
                     /locus_tag="IW651_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813997.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            33322..>33509
                     /locus_tag="IW651_001258"
     CDS             33322..>33509
                     /locus_tag="IW651_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0813998.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        12025 a         5164 c         6841 g         9479 t
ORIGIN      
        1 ctgatattac ttcttttcag gcagtgctgc taatattcaa ttacactttt ttgtataaat
       61 ttagttgttt taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttctgcac
      121 ttttggacga taaatttgga caattgaaaa tacaaaaaaa agaaaggccc tgtgagactc
      181 ggaaggctac caaccttcgt cgacgtccac aggtggcatt aagtctatac cccttaatgc
      241 gtttaccttt cagtgttacg gtattacccg taacggattt agtataacag atattcaaaa
      301 gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta
      361 tgcctaagca taatgttata aaaatgtccg tggggctctt tgccgtatga cgaacgctga
      421 aattagaggg aacgcatgtt tgaatgcgtt ccctcttttt tgtttgtcat gaacgaaaat
      481 caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagtaagtgg
      541 gtggattatt ttatgtcata tacaggaatt ttgagtctcg aagatatttg tcactacggt
      601 aaacggtgta ccgctacaga aaagatcacg aagaaactat caactggtca aagtaaaacc
      661 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagaaga aatgatttat
      721 tacattggga aacaaaaaca aataatttta aaagatccta tccctttaaa agagctatat
      781 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
      841 gtcttgcgtt gtaccgcaaa agggatgggg cgtttaatca gctgaattga tcattctcat
      901 aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact
      961 tagaatcact agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg
     1021 acggggtagg tttttacgat tggtaacgta aaaaattggt caatcgagta aatgcccttg
     1081 tttggatagt ggtgaaatga atttgtgtaa ataatatgca cagagtaaac aagagggaat
     1141 ttagaagtta gacaagttag aatagcgcct agattaatca tgagcagcta ctaagggatc
     1201 taggctttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca
     1261 caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgatcattgg
     1321 atcaatcgag catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt
     1381 cgatttattt tatatatttt tgagtgaacg aaaaacgata aagatattcg ttatgaagta
     1441 aaactaattt agtagatagc aaaggtggta gaggaatgat tcaaaaattt gcgaaaatga
     1501 gtaaaactgc atggattgaa aagcttgaaa atgacttgag tgtcatgcga ttagctccaa
     1561 agaaattcaa acgagagatt gcagaaaaag aagcaatttt gtcggtattg aatgcggtag
     1621 acgataaatg tccagtaaaa attagttttt cagatatagc aacaattatc gaaggtattt
     1681 attatggtga agttgatgaa gagatatctt ctacaaaaga tttattagat tttttggata
     1741 aaaacaaaga tacctttata gtactaactg attatgagga ggttacaaca aatgaaaaat
     1801 tatgatccga atatacgatt gggcacacat acgattaagg tttctttcca acgttgggat
     1861 tacaaaggat ttgtgacgtt tagaagaggc ggaaattgta aaggattaga tgttttagca
     1921 ttagacgagg atgatttata cgatcaaaca ttgactgata atccaattgg attcggcttg
     1981 ctacctgaag atgatgaagg taatgaatgg tttaaaatga cgcttacgga tgataatgga
     2041 gacgaattat ctgttgaaga tacttggtca tatttgagtg attatattgt cagcgttgaa
     2101 atcattgaat ttgtagcgga taaagaagaa taaaaactgg aggaataaat tttacagata
     2161 aaggcttaga aggagtcacg acgaataata aaaaggtagt attaccttag gcattgagta
     2221 aggaatttat ttaggaggaa aattagatgg cgcgaccagt tttagaggcg atggatatgg
     2281 atactttgac aaaaggaagg tatgtacagc tgttagctcg tggttattca cctaaagatg
     2341 tattcaaatc cctgtctaaa ggaacagata tggaaaaaga aagattacga aaagaattcg
     2401 attattggag aacccataat ggaatcaagg atttgaaacc agcacggcca gtaattaaac
     2461 gaaagaaaaa gataacccaa gactgaagtg tagagcgtat gtgatcatag gcttttttat
     2521 ttttgcaaaa aacaagaaag gtaggaaaac tcatgaatta tgaagaaatc gaaaacagaa
     2581 aaaaagtgtc gaaagaaatg gaagaaaagt tgctaaaaat gatgaaacaa aaacacctta
     2641 aaagattatc tgtcatgcaa tatatcaatg atatgaagat aacaggaaaa gagaaagctt
     2701 gtttgttagg ttcaatgaaa aactttgaac agttgaggcg aacttatgta aaaacaggtt
     2761 ccaattgcca actattatta gaagtttctt gacgattatg atatgaaaat gatcaattat
     2821 gatgtggcat tgttattgaa taaaaagaat agtaaaattt aacatggttt atcttggtta
     2881 gatgtaggta aattaattca tatatagaag gtagatagat gaataaaaac aataaaactt
     2941 tatttaatcc ttacgtgggc gtaatcattg tttttcttct ttttagttat tttgcttatc
     3001 aaggttatca aaaggacaaa atcgctgaaa aagtagatgg tgtaacaaaa acaatccata
     3061 ttcaggaaca ggctaaaacc tatcaagata atttaaccag tttaacggat atttctaaga
     3121 gacaactgat tactaatcaa aaacaggcat tagaaaagga tctcaaaggt gaaattgctg
     3181 gaaaacttgt tttttcttct acaggattta ttctccctgt ttttcaagga gcgaatagcg
     3241 acacgcttag tttaggagta gcttctactt attatctaga tactgaaatg ggaaatggaa
     3301 attatattct tgctggttat aacgtagata caccaagcgt tttattatcc gatatgactg
     3361 atttatctga aggagaagaa attgatctaa tcgatgctca ttacaagtat cagtatgaag
     3421 tctccaaaag aggaaaaatt tctgattaca gccaggtagt tcaatctaag gaaaacaatt
     3481 cctttttatc cttgccaaaa gcaggtaaaa aaccactact cacactgtta gcctttcaag
     3541 caaataatat gaaaaagtgc gatgtgatcc agtgtgaatt agtcaatgta gaaaaaatca
     3601 ataaataagg gaagtgagtc acattcaaga acttgtagaa aaattgatca ataaaatgag
     3661 tgccattttt ctgttactgt tggtatccat tctatattta atatggacgg tggggttaaa
     3721 tcctcctacg atattattta tttatgataa tgctgcacag ttagtgacgt ttttcgtagg
     3781 ctgtttgttt cttttgaatc tgtacaaggt aaagagaaac gatctattct tcattgcagc
     3841 aggcattctt ttttaccttt tcttttcttt ttttagaagc taccgaggag atactttcta
     3901 tggtgatctg atcgttccga caattattgc tattattttg attgtaaaat ggacgaaatt
     3961 tactgtaatt gatcgtagct tgtatcttgt agtattctat gtctttttac tcgtaacggt
     4021 ttatcgagta tttactgaga taccagtacc agaaggacaa agtatttgga atccatcgaa
     4081 caaattatca gatatttgga tcaatacaaa tacgataggt agttctttaa tgacattagc
     4141 ccttcttatc acaggatttg ctagttcatt tggaaaatgg tacatgcgat tgctatcatt
     4201 accagcgttg attcttgctg gggcaacgat ttgggtttgc caatcgaaag cagcattata
     4261 tgcgcttgtt atcttcatta tcttagaaat tttacctaag aaggtttttc gaaatacaca
     4321 cttgtccttt tggatgtatg cagcagtttc ggttctagct ttaccgattt cttattttgc
     4381 tgcaatgtca gacaaagttc acttatttac aggacgagaa gaaatttggc gcaaattcta
     4441 tgaaacatta ggcgaaaaaa caggacaaat atttgtaggg atgaaacctt tctttttcca
     4501 tagaggaaat cagattctag gtaatcataa tacgtatcac gcactcgtca atcactatgg
     4561 attgattggt tttgttctat cgattattct tttgcttagt ttcttatggt tgctcgtaag
     4621 aaaacgtgag ctgacaaatg gacaactaac attcttgtgg gcttttttag cgatcatgtg
     4681 tcaatcgttc atggaagaaa cattaacgac aatcagttgg gtgccaattg tattcttaat
     4741 gttagcaatg gctacacacc aatataaaac caccagataa atgaaataga atagtaataa
     4801 gagaaaacac tttttcgttg tgaaaaagtg ttttttgttt tgtctgaaag gaggcagatg
     4861 gatgaaaaaa tggttatttg gttttttagg agtcgcttta atcgtcgttt gcagtgtgtt
     4921 tggctatgtt agctaccaaa aacatgaagg agaggtgttt aagcaaaata tagaaaaaaa
     4981 gatgcccgta gatcaaatca atgcacacgc aaagagttat aaggaggatg caacgaacgt
     5041 caataatgat atgtctttgg ggcaaatgtt gtccatacaa aaagaagcaa tcgaaatggg
     5101 cgtcaataaa caagtatttg cacaaattca gattcctgca cttggtttag cattgccaat
     5161 ttttaaaggg gcaaatcaat acactttgag tcttggagcg gctacttatt tttatgaaga
     5221 tgccgaaatg ggtaaaggaa actatgtcct agcaggtcat aacatggaaa tgccaggtgt
     5281 tttattttct gatattcaaa agttgtcatt aggggaggtg atggacttag ttagtaacga
     5341 tggcgtctat cgttacaagg taacgcgtaa atttatcgta cctgagtact ttaaattaat
     5401 cgatggcgtt cctgaagaga actctttctt gtctttacca aagaaaggag agaaaccctt
     5461 attaacgttg tttacttgtg tttatacgtc acaaggaaaa gaaagatacg tggtacaggg
     5521 agagctacaa taacaagaat ttaatcaacg cgagtcgttg aaatatgaaa tgaggagagt
     5581 aaaaaatggc aataaatcgg tatgtaaaaa aagctggaat gattgttgga atgttaggaa
     5641 tcattgcagg ctgctttcag gggtatcgag cttatgcaga agatgtcacg caaaaaacgc
     5701 caccagaaag ggtcaatatc acggttcata aattaatgta cgatcaaagt acgcaattga
     5761 acgttgatat tgacggaatc aaaaatgatg gttatacgca cgatcaatat cctacaggtg
     5821 taacgaaata caacaaggca gattatggtg atgttgagtt tacgttagga aatattacgg
     5881 atcaagtatt accgactgaa gattctgatc taaccaacgc gaaagtggac gaaatcgtta
     5941 aagatgtaga agacaaggga tctaacagtg agtacgttaa gaatgcaacc aataaaatta
     6001 cttcagctgt agatgaaaat ggtgagatca cttttgctga tcaaccagcc tatgtcaatt
     6061 ccaaagggaa tgtatatgtg gtttatgaaa gtaaatccgc tgctggatta gtcacacaaa
     6121 aagcaaaacc aatggttgtg attgcgccaa tgacagacaa cacaggatca ggatttttaa
     6181 aagatattca tttgtatcca aagaacattg tttctaagtt gagttttgaa ttaacgaaat
     6241 ttggtgacga tggcacagct caaagcaaac aaacaccttt aaaaggggca aaatttgagt
     6301 tatacaaggg cgaacctgga aaaggaacaa aattaggaga gcttgtatcg gatgatcaag
     6361 gaaagctgac tgcaactgat ttgacgttag gaaaatacta ctttgtggaa gttccttcag
     6421 aagttgtagt tggatcagat caagaaccaa cagcagacca atatttgtta ggcgcagacg
     6481 ctcgcaacga tgcgcacaat aagttaacct ttgaaattac gaatgacggc gtgacaagtg
     6541 atttaaaagc ttcctacgtg aactataaag caccagtgat cgataaaaca gtgacaaatg
     6601 gaacaggaga agaacattct ttccaaattg gtgacgcagt gaattatcaa ggtacaattc
     6661 atatccctac agatatcgca ggtggaacag atgggattac agtaaatggt gtaaaaagtg
     6721 aaacttctcc ttacagtgta tttaaatggg gcgacacagc aggtaaagga ctttcttatg
     6781 ttgctgaaaa agcagatatt aaagtaacaa ataatgatgg ttcagtcgta ttaaaagaaa
     6841 atactgacta taaaatcgaa aattctgata gtggttttgt gatcgatttt atcgtaaata
     6901 atggacaagt aagtgataca gttgcgaatt tacatggtca agatctaaaa atgacttata
     6961 acatgtttgt gaatgatagt gctgaaatcg caaatccatt aacaaatagc gtggatttcg
     7021 tttataacaa taatccattt aatcaggaag aacaccacga aaaaacgaaa gctgacgttg
     7081 tgacttatgg cgcaaaattc ttaaaagtcg atagcggcct atttggaaca ggtatcaaag
     7141 caacaccgct tgaaggcgca gaatttgcag ctaagaatgc agaaggtaag tactacggtg
     7201 gacttactga tacagataaa gatggtgtca aagaagcagt ttgggtagac gatgtagcaa
     7261 atgcagcaat cctaaaatca gataaagagg gtcattttga aatcactggt ttaactgaag
     7321 gagattactc actggaagaa acaaaagctc ctgaaaacta tcaaaaatta acaaaagaga
     7381 tctctttcaa agtagacaaa gattcataca aagaagaaaa ccgaatcacg atcaaaaata
     7441 atcaaaaagc ttccgtacca atgactggta gcaacggttt ccaatcttac gtattgatct
     7501 cttgcttgtt gcttggggca ggcgcaattt ctgcagttgt ttactttaag aagaaagcct
     7561 agaaggatca gctaatgatt atcagacacc ctaagaaaaa gcgaatcatg ggtaaatgga
     7621 tcattgcttt ctggttactt tcagctgttg gtgtcttact tttgatgcct gctgaagcga
     7681 gcgtggcgaa ataccaacaa aatcagcaga ttgccgcaat cgatcgaaca ggaacggcag
     7741 cagaaacgga ttcttctttg gatgttgcta agattgagtt aggcgatcct gttggtattc
     7801 ttacaattcc aagtatttct ttaaaactac caatttatga cggaacttcc gacaaaattt
     7861 tagagaatgg tgtagggatt acggaaggaa ctggcgatat tacaggagga aacggtaaaa
     7921 atcctttaat cgcagggcac agtggacttt ataaagataa tcttttcgat gatttgcctt
     7981 cagttaaaaa gggagaaaag ttttatatca aagttgatgg tgagcagcac gcttatcaaa
     8041 tcgatcggat tgaggaagtg caaaaagacg aactacaaag aaactttgtc acttacttag
     8101 aaccaaatcc caatgaagat cgagtaacgc taatgacatg tacgcctaag gggatcaaca
     8161 cacaccgttt cctagtgtat gggaagcgag taacctttac aaaatcagaa ttaaaagatg
     8221 aagaaaataa gaaacaaaaa ttatcttgga aatggctttt aggaagtact gtttttcttt
     8281 cagtcatgat cattggttcg ctatttgttt ataaaaagaa aaaataaagg agggtaacca
     8341 acatgagaaa tagaaaatgg ctatttgttt gtgcagccgc tcttttttct ttcactgcct
     8401 acacacagtt gacgtttgca gacagacaag aaagcacgga tagcacagaa caaacaatcc
     8461 aaacgtcaga tagcagcacg attcaaagca gcgatgaaac cgtcgattcg tcaacagttg
     8521 attcttcaga agaacctaaa attgctgaag acgatcaaat agtcaaacag accattcacg
     8581 ttcaaaaaat tatttcttcc gaacaattag acaaccaggg gcaaaagctt gaaaaacaag
     8641 aaggagtaaa tggcgcgaag ttttctgttt atgatgtatc ggacatttta caaaagatgg
     8701 atgttaagga tctcactact gaccaaattg aatcccaatt aaaagatcga gtgaaaaaac
     8761 tatccagtga tcaactgaag ctaatttcta atggagaaac aaagacaatt gatcaacaaa
     8821 caggtgtctt tgaattctcg gtagaagtac aggcaaatca gaaacaagct tattatattg
     8881 tcaatgaatc aagtcctgaa aacatttcta atagcgagga tattttgcta ttaacacctg
     8941 tttctgataa aaatggtgaa ttcttaaagg atgtgtggat ttatcctaaa tcagaagctt
     9001 cgcaacctaa agaagaagta aaaaaaatcg tatctactgg cgtaaagaaa aacttctttg
     9061 agaattgttg ggattttgtt actcacttgt tttctagaga ttaaggaaga aataaaatga
     9121 aactgatcaa attattatta ttcaattttt cttgtttaac cgcagtcttt ttgtactgcg
     9181 gtttattatt ttctaaacta tctattttgc caagctacca actataccta atgaggattt
     9241 taaacatacc agctgcttcc atgccgctag atattatgga aaagctcatt aggaatccat
     9301 tatttactac gatcaatttg atcgttattg tcggaatttc actagtcatt tataaaaaag
     9361 aggggatgaa gggatgaatt ttaaacactc gtggacaaga cggttaaaat atgtttttcc
     9421 cataatgatg atgttaggta cattgtttgg gttaaaaaca gccaatgtct ttgctgaaaa
     9481 agttattgtc gatccgtcaa atccaatcca gaacgtaaag aataatttta ttattccaaa
     9541 agatgttcct aatgcaaaaa caaatattac tgtaaacgga gcgtcaaaag tttacaaata
     9601 tcatgttgat gccaacaaag gtggttttac agacgactac gtaggattag tggagggaaa
     9661 caaaaatata cgctatcctt ctttccaaaa agaatacggt gaaacaaacc ttgttttaga
     9721 aggcgtaaca accaacacca aagtcaatat tgactatgga aaaatcggta cgtataacgg
     9781 gaaaaaagtc aatatcaagc tagttctatc taatattcac ttatactcgg atacgttgcc
     9841 gtggaatcta ctagataaca actatacaaa aactcatttt cgagacgatg gctataaaaa
     9901 tacgaatggt gcaatgagta aatctaaaaa aagaacagta ctttggatct cagacaatct
     9961 tttctcagga attgtttacc attcaacaca aatgaatgtg cagctagtag ccacatatga
    10021 ggacggtagc cccgttcaat tttcaggaga tacatttatc agttttaact ctttgaaccc
    10081 tgcaggagga aaaagcacgg atcttaaagg agaatatgcc cactatgaca aaatgaatac
    10141 gacagattgg tatgtcagaa aagatacggt tctttcagaa tttaaatctt tctacaacaa
    10201 tctgaacgtg gtaggtggcc acccaggagg aagctctaag ctgacacaag cggataatga
    10261 tttcaataat ttgcatgata aattaggaga tccgaagttt ggtcaaggta ctgtgtcctt
    10321 taaaatatct gaagcaaatc cgacgtttgt gattggttct tctaatgttc aaacctggtt
    10381 tactttatct tctgcgacaa tctttagtgt tgttcctgat caacctgaaa aaacaggggt
    10441 tgataaaaat ggaaataacg tgaacgataa aatgttgcaa gtaggagaca cgattcaata
    10501 tcgaatcaaa cagaaggtga atcgattagg agtggattta ctagcggttt acgatcgatt
    10561 tgagttaatc gataatttac caaaagaagt aaattacgtc gatgcacacg ttgaaagtgg
    10621 tacaaataaa aaattcgatg tatctggtga agtgacctat gacaaaacaa aacaccaggt
    10681 aaaatatgca gcgaaagctg atacattaaa aaatcgaatg aagtataacg gcgaaactta
    10741 cgaattagtg atcaatgtaa aagtcaatga attggccaat caaaatagtg tagccaaaaa
    10801 tcagggaacg tcgatcatta ataaagtaga aaaggataca aatatcatta ccgtttattt
    10861 ccctaaaatt ccagtgaaag aagtacaaca aaatggaaaa gatgtaaacg gacgaaatga
    10921 cggtaagaag ggtacgccga cagctccttt aaacgcagga tctgaagtac aatatctagt
    10981 cacacaaaaa tggcacacaa aaggcgtaga cgctgcgtca gatcactaca aacaatttag
    11041 tattcaagat ccaattgaag ctcgtttgac atataaagaa ggttctgcgc aagtaataga
    11101 taagagtaaa ggaaaagata ttacttctga aggcacacta acctatgata gcaattcaag
    11161 aacgctgaag tgggaagctt ccgctgactt cttaaataac aatcttttag atggacgaga
    11221 aattcagctg atatttacag ctaaaactcc attacaatca gaaaaaaata ttgataacca
    11281 agccgtagta gcagtagata atgtttctaa taaaacaaat gttgtgacaa ttggggttga
    11341 tcctaacttg ccacaagtta ttgttcctaa aacaggttct acacatttag taacgatttt
    11401 agcggtcagc ttactattac ttgtgttagc aacttttggc tacgcagttc tgaagttcaa
    11461 ttaaacaaaa aaacaaggct gaaaatcagc cttgtttttc tatttgaaaa tgggattggc
    11521 actaataaat acgttgtctg aagaaagtga tccgtctgca gaaacttcat aagcatggga
    11581 taaaccaacg atactttgat tgttgtaaag caagtttgcg ttatgagcag cgctatcttt
    11641 ccacataacc atagcattta ctgctcctgt atctgaaagg atctcgtcaa cgtaaaggta
    11701 gaaactattg atattttctc ctttagcacc gtagcagcct aagctttcta agagatcttg
    11761 gaaagtaccg ccgccaggtc ggtaatgact gaagcttact tgtaattctt ttgcacgaat
    11821 atctgcagct tgttgaacga tatcattgaa ttgcattggg gcaacgccat tttgttgacg
    11881 gtagctattt aaccaagcaa gttctttttg acgcacatag ttgtttaaat cacttagtga
    11941 gatatttttg tatttgaaag tgatcgttcg attttggtca acattattga ttacacgttg
    12001 tccattttta tcaacggtaa cagatgaatc gtcatttcta cacaagaagt agttttgatc
    12061 ggtcatattt ttccaagaag tagagaaact atctccctcg taaaccgttt gtgtttcagt
    12121 agataaaaca gcgtttgtgt caacgtttat atggttcaca gtgacgttgt attgatgagt
    12181 ataggcaaaa ttgattcctt gattacttgt aacgttgttt aaagtcacag tgttgctaga
    12241 gctttctcta ggatcaagaa caaaattagt gaacataggt gcttcaattg tgatgctttg
    12301 tccataaacg acgggataac tcttactgta attatagagt tcttggatat ttggatcagt
    12361 atccgagact ttatttggac gagctttcac ttcaactaga aagacttcat catagtaaaa
    12421 agtaaatgtg gtatttgtgt taatcgtttg tgtttgtgtt tcattcccat ttacttgata
    12481 ctgtggtgct tcaaagttag tcaagtcggt tctagctttt gcagtgtaac ttgtaccagc
    12541 ttttactgag acatgttctt cttttagcag cgttccagtt tgtcgattca tatgacggac
    12601 agttaccgca tattctgaag gtttaggttt gtgttgtaac cctttaaatg cagaatcaag
    12661 actattttta gcattattta cttgggtttg agaagcttta gaatcgtcta gtactttttt
    12721 agcattggat aaagcagtct gaaaattatt ccaagatcct tctgtaaaat ctcctttagc
    12781 agttgatttt acttggttgt acaaggcttg taattccgtt ttgttcacag taggagctgg
    12841 ttttttcttt aatcccttgt aagcagaatc taatgtgttt ttggcactat ccacttgttt
    12901 ttgagtcgct tttgagtcat ttaacacttt tttagcattg tttaaagcgg tttggaatgc
    12961 gttccaggta ttgtcggtat agtcaccttt tttagttccc ttaacgctcg tataaagagc
    13021 ttctaaagct gatttattca ctttgggttt agaggtgtca gcaccatacc aaccaatacc
    13081 ttcgtatttc cagccatagt ctttggttaa ccattgtact tctttccaac ttgcagtgta
    13141 gtgatgcgaa ccagaggtaa cacctggaag gaaaaggcgg tagatcggtt tattattttt
    13201 ttcggcagaa cgccaggcag gcccttcata agtccaaccg taatttttgg tcaaccattt
    13261 cacttcatcc caactagctg tgtaatggtg atcacgaagg cctttattgt ataaacgata
    13321 gacaaggtgg ccaccgctag tcggtgcttc ccaagcgggg ccttcataat taccccactt
    13381 aattttcact aggtgatcac gttcttttgt gctagaggtg taaaaatgtt catggttatt
    13441 tggattataa aggcgataca tgacgttagt tgaagcagcg tcagcagatc cagcaagagc
    13501 aactgtggca gcaaatgttc caatagctat aacagttttt gggtttaata atttcatgtt
    13561 ttcaactccc tattaaatga ttaaatattt tgtttacatt cataatatac cacggtatac
    13621 cgaggtagtc aacggtatac cgtggaaatt ttgaaataaa aaagccgccc tatgaagagt
    13681 gtaaggatgg ctgtttatat gctatgaaga aatggattcg gcgtatttgg ttagtttggc
    13741 agcattatca aatcgcattt tttcaatcgc actttttttc tttattaaat ccgcaattgt
    13801 tgattctgcg actgaagaat gtttgctaat ttcatatgct gtcttgccag tttcaaatag
    13861 taaccattcg attttctctt tatcagcgta cattgcaatg ctccctcttg attttttctt
    13921 tattatagct cttttttgta ataaatcaca atatcaagag gaaaagtttc tatagaaagg
    13981 tgggaaatta tgagtatccg ttccccgtgt atcaaaatga ggtttgttgc tactttcgtt
    14041 atttaatata ctaaaataaa acaacgaaag aaggaataga atgaaaaagt taataatggt
    14101 atttacttcg ttgattgcca tgattgttat tttaggtgga tgtggtagtg aaaaaaataa
    14161 gcaagatagt caagataatg gcgtgttaaa accaggaaca gtgtggaaag aagaagtagg
    14221 cggacttgtt cataatttaa aaattataga tgatacaaca tgggaatatt ccgaaagtgt
    14281 ttggcatcct gaacaagtac aaataacagt tgagaagcta aaagactata aaggattaga
    14341 aagatataag gtaatcgatt caggtggagt agatttattc ataggtaaaa cagccttaat
    14401 agttccctat aacaaagacg gtttagaaac gtttaaattt attccaacaa acgaaaaaga
    14461 acatcgagat aaaagtgaaa tattaaaaga atacagtact aaatctggat acaagttaca
    14521 aaagactagc gagtgattgc tagtcttttt atttatcctg aaggaggaat cgcagtggca
    14581 aaagctaaaa cgccatttga aaaagccgcg tctaaaatct tagaacgtcg cggtgtatct
    14641 gaaacacagt ggaaacaaga agtccttgaa aaagcacaat ttgacctatt acggggaaaa
    14701 gacaaggact tagaaggata tatctatcaa cgtgaacgtg aaaagcttgt tttcgatgaa
    14761 attattaaac actaaaagga gcgaacaatt ttatgtttga atcaattaca atgaattttt
    14821 taacacaacc gaaagtagta gctgcggttg atatttttag agcattacaa ggtgcagcaa
    14881 ctagtttaaa aaacaatctg atgatttttg cggctgtagc tgccgttgcc tccattgttc
    14941 ttggggggat tttcttcttc tttggccgaa gaggagctga aaacgggaag atgcacgtag
    15001 gttatgtatt gatgggggta ggagttatat tagctgccgc ctctattgtg gtttcgttat
    15061 ttggaatgtt tggacaaacc ccacagggac tataaaaaat aaatagaagg agtaaaaagt
    15121 acgtggctac tttttactcc ttttttatga aaggagaata ctcatgtact ttacgatttc
    15181 aggcaatacc aaaaaagata aagagttaat aaaaaaagca acgaagaaag tgggaaagcg
    15241 tactgccttt ttaccgaaaa caattaaata ttctgtcatt gaaacggaag aacagctaca
    15301 tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga ctctcacgtt
    15361 acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg gcccactgga
    15421 ttatcaaatt tcagaaggtt tttatcctac attgatggat ttgatccgcc ttgatgtgtt
    15481 taaggaaacg gatgaaacca tgagtgtgga agagtcttat cgaaacgaaa aactgctaca
    15541 agttttagaa gaagagttag agagcgacca aagaaaagtg agtcaagtga gtgaagtcga
    15601 agtaaatgat gaagtaccat gggaacgacc aatctttcca aagaaggagt caaaagaaaa
    15661 agagacagtc tatgttgacg aacaagctga tattccgact cagaaatcga tatttgagga
    15721 agagaacgat gtaatgggtc aattggaggt agatcccttt gaagaaacaa tggaggatga
    15781 agagacggcc gaagtagaag ttgatctagt tgaagctaat gaagagccaa catggactga
    15841 aggggattct caggaatata ttgaaccact tatcagtgaa gaagtagaga aaaacgaaac
    15901 agagatacaa aaggcaactg tatttgatta tattttggat caatatccgc cagagcatga
    15961 atggatgaaa gaaaaaaata aattattttt aaatgagctt tattcgaaat atcaattacc
    16021 ccaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg gtaaaggcac
    16081 acttttagaa cagttagagt ctgttcaatc aacaaattgg catgagcttg cacaagaaga
    16141 tttattagcc acttttgaag aacgtcaaca agaaacagat caagaaataa atgagtatgc
    16201 gacggaacag tcatctaatt gggagagaac aaaagaggaa ctaaataaag aagaacaacg
    16261 agtgattgaa gaagaagtac aaaaaattaa agagcgatac gaaaagaaac gcgacaatgc
    16321 gtattccttg tgtatagaga aaattaataa ctttactaaa actaatcaag aagctttatc
    16381 gaaagaaaaa gaaaacttgt taaaagaacg tataaaagaa cgaaaagaag agttctaccg
    16441 aggtttacgt acagataagc taaagattag caaagaatta aatgaacaac tgaataaact
    16501 ttatcaaaca acgactgaag ttcttatgga aagacacggg cagatccagc aagaattaca
    16561 agcacaaatt ccaaaatgga aaaaagagta tgaaattcaa caacaagaat tgaaagaaca
    16621 agaagaaaaa caacgacaag aagaaaaaga gcgcgaacaa ttagaactaa aaaaacgtga
    16681 gcagcatctt gcagaacaaa aagtggcgat agaaaaacaa cggttagagc aagaaaagga
    16741 aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagcaca
    16801 gttgaattat ctagcacaac ctcaaccagt agctgctgtt caaacgccag tgaacaacca
    16861 agcaaattta actccttcac cacagcgttc ctttaacgga tggatggtgg gatgtattgc
    16921 aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata tcaatcaatc
    16981 ttcacaggca gattctgtag ccacaacggc gtcaatcaag gaagaagccc aggaacaagc
    17041 aagaaaagag tatgaacaac aattggaaga attattgaat cgtaccaatc agtcaacagc
    17101 agagtcaagt atggaaaata gcaaagagag tgatcctagt aaggctaaag aaggatcaag
    17161 cgattcaact gtacactcaa acacttccga aagttcagaa tcaaatgagg tgaaataatg
    17221 cgtattagag aaacaacgag acgaaaagac aaacgattca atttagaaaa gaataggtat
    17281 ctttctcatt tctatcgcta tactccttca ggagaagagc taccagaaaa acatgacaaa
    17341 cgtatttctc aaaagatttt ttatagtggc ttagcttatc ttcctttaaa ctatgttgtt
    17401 acaagaatac cagaagcatt tcatcgattt gagattattc caggaagttt acaaggagtg
    17461 aatatttttc agttacaaga aatgccgcag ttattccatt ttgattcaac tggtttttgg
    17521 aatcctttga ccccaacaac aagtttaggc acacgtttag ttgtttctac gatttgtgtt
    17581 gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtccgct agcagatggg
    17641 caaaaaggag ataaccgatt aaaaacgtta aaggaaatcc aacaaattta tccgcaaata
    17701 ccagaatcaa agggaagttt tcctggttat ggcggaatac caattactca ttataaaaaa
    17761 tactggtata ttcagacgga tacagtaaat acttgtattg ttggtacttc ccgctcaggg
    17821 aaaggccaga tagaagtttt agcaaccatt gacaatttgt cgcgtgcaga aagacaaagt
    17881 tcaatggttg ttaatgatcc aaaaacagaa ttatatgttg ctagtaaaga tgaactagaa
    17941 gctagagggt atgatgtgta tgcctttaat atcaccgatc cactgcaatc aatgagtgat
    18001 aatccattgg cactcattgt gaaatactgg aagcgaggag acatagacac cgcaacacag
    18061 ttaaccaata cttttacgaa tacgatttat tatgatgcaa atgcttcaga caataaatac
    18121 tttaacgaaa atgcgcagaa agctgtcaat gcgcttattt ttgcgttatt ggaacaagcg
    18181 gatcaaacag gggattactc caaattaaca ccaaataatc tcgttgaact actttcagaa
    18241 attggcggtt ttaattatta tccagatcct gagaatgaat tcaagcagct caatgcctta
    18301 gatgagttca tgaatcagtt accccctgga aatatagcaa aaaaacaata tggggcaaca
    18361 aaaattggat ctgataaagc gaaagggaat atcttgtcta cggcaattac aggattgaat
    18421 ccatttaccc taacgaaaat tgcgaagatg acttctcaat cgaccattga ttacaagtcc
    18481 attggttttc cgaagtattt ggatctcaaa ttacatgaaa gcctattaaa tcaacgcatt
    18541 gtcgtggaat tttatcataa gggaaagaaa tttcacgagg aacgagtcaa agttggctat
    18601 cgtggttttt gtgaaatcaa ctttgattgt catttagagc agggagatca tgtttgctta
    18661 aaatacaatc atcaaggaaa acaatatgtt gcttggtatg agttttctca aaaagtattg
    18721 aaagacgaag agggaaaaat cgtttatcga aaaaaacgag gagaaactca tttgccagaa
    18781 ctagaaaaag aagccgttct aacattagat gagaagaaaa gtaacctatt agtagagtct
    18841 attcggatgc actataccga taaaccaatg gctattttta tgttaacgcc tgattatgat
    18901 ccatctaacc atatcattgt tagcatcttt cttagtcagt tgtataaaga attatctacg
    18961 caatgtgtta agactaaagg agataaatgt caccgtagga ttcattttat tttagatgaa
    19021 tttggaaata tgccaccgat ggacaacatg gaaggaatca tgacggttac ggcaggtcgt
    19081 aatatgttat gggatctgtt tattcaatct tatcagcaac tttatgcgaa gtatggagaa
    19141 caaaacgcag cgattatcaa agaaaattgt cagacacata tttatattat gtcgacagat
    19201 gataatacca tcgaagaatt ttctaaaaaa gtggggaata agacggttga acaagagaac
    19261 agtaccatca attcattagg tatgaatact catatcaatc gcaatgttga ttcagatcgt
    19321 atcttaactc ctgaaagaat ttctactttt ttagaaggtg aaacgattgt actcgctcct
    19381 ttaaaacgac aagatttgaa aggtcataaa atacggccgt atcctatttt taatcatggg
    19441 gaaacgaatc ttccttatgc ttatcaatat attgaagatt tcaatccatc gtgggatatc
    19501 aatgatatag acatacattg tacacatgcg gacttggatc ttaaggcttt acaaattgat
    19561 tatgattatt tcctaccaga tgaatatgct agaaatctct atcatggact gcatgatcaa
    19621 ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg ttcaatcttt tgagaatgag
    19681 acggctaaaa aattgttaat tcaattttac gaagaacaag caacggatcg tcttcaagaa
    19741 tatgttgaaa agttgatcaa aaatgaagaa atttctgaaa tgattaggaa acggctgtta
    19801 ttggaagctg aaaaggcggt gaaagaatga tcattggagg aattttctct ggcatagccg
    19861 actggtggtc aggtttattc ggagaagggg atgcagatcc caaaaccgta acgaaagtct
    19921 tacaagatta tggtaattat ctgcagtatc aagatgtatt cagttggatt tgtaatactt
    19981 ttttatgggg attgatcaaa gggttatata atctcaatac tttattagaa aaaacaattt
    20041 atcaaagctt tgatttaaaa gacttattga acgccgctgg tgttaatgaa ctatttcaaa
    20101 gtttgattag taaggtattg gctctttttt gtgtcgtcac attaatttat ttaggcttga
    20161 aattttttat gagtaaacac ccaccgaaag tcaaaaatat cttggttaat ttatttatgg
    20221 ccatgatctt gatttttggt ggtagttctt taatcgatga agggttaaat atcagtaaaa
    20281 acttttatgg agacgtcact gcagcaaata aagcagataa aacgggttca cctgcttttc
    20341 aaattattca aaataatgtg attgatgtca gtacattact agcgactgac ccgaataaag
    20401 tagaatcctt accaacagat aaaagaaatg ctttaacagc gaaaaatttc aaaacagcga
    20461 atatcaatgg gattattaca cctgatcaag ctaagaaaat gtctgaatca agtgatatta
    20521 gtacagtaca aaaagatcgg ttaaaggcat tacagtatcg actagagtta gacgataatg
    20581 ggaaagaaat ccctgttcaa attagtgatg aaggtttagc aaaatatgtg tatacgtcag
    20641 gctatcgtcg atacattagt agtccaggga ttattttggc tggggaatct tctttgtcgg
    20701 tagcctattt atttattttg tttacaatcg ttacttgcat tattgagcta gtatttaaaa
    20761 aattctattt agtaattgcc gcgtcaacgg actttgagac aggacaacgg atgaaaagtg
    20821 cgatagaaga tatttctcaa tccttcttgt tattggcgtt tacgatttta gaattacgaa
    20881 tctatacatt aatgctttca ggtatcggtg atttgcatgc ggtaggaaag atcaatggat
    20941 tcttgtatgt tgttgcttta attgtgctaa ctattgcgct attcaaaggc tctcaatcgg
    21001 ttacgaagat tttcggtgtt gatacgagtc tgaaaaacgg cagcaattct cttcttagca
    21061 tgtttgcctt aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg
    21121 ctggtaagag tggtttaagc catttaaatg atattcgtaa gaatggattt ggccatcgaa
    21181 aagatattac ccatgataac ggggaagaac aatcagacga taaagctaca actgtaccta
    21241 atagtaagaa gacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg
    21301 cagaaggtct tggttacgtc aatgaacgtg ggttaaaagg aacagttgaa gatcttacag
    21361 aaaaagccaa agacacctta gaagagaaat tagacggcac gagagctaaa gaatttgctg
    21421 aagctgtgaa gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagcagtca
    21481 aagaaggact aaaagatgcg tatggtgaag gacaagtcaa tgcagcagtg aaggcaaata
    21541 caacaagtga aaaagaaaat caagaaggtg cagcgattcc ttcagccaaa gatcaccaag
    21601 atgagttaca ttctaacgaa aattcgatcg gagaaaaaga ttcagaagaa ggacaacatg
    21661 gtcagttacc aaaaaccgat ccagaacaat atcgttataa atctaacatt aattctgaaa
    21721 acgaaaaacg catttcagat ttaccaaaac gaaatcaaga agtacaaacg gatgaggatt
    21781 ccatcgatct gccaaatgat aatggcatcg aagttggaac tgaaaaatca acgaaaattg
    21841 gtttgcctaa gacacaaaat ttaccaatga aagatagtcg tttgaatcaa ttcaaagaga
    21901 atgatcaaag ctttgttcat caatcacctg caggtactgt tcaaatgaaa tcaagtcgtt
    21961 tgcctgaaaa tggaaatctg acaactgttt cttacaattt agctgaaagt ggtgaaacag
    22021 agaatgcgtc cgttcaaaat attgtgacaa aagaagctgc acctaagcca atcaataaag
    22081 ttcataatgg atacagacca gcaaacgtag catttactgc accgacaaaa ataaatgtga
    22141 caagagaaac taaaaacagg aacaatgaat tgaaagaaca actattgaag gatatcaaac
    22201 cataaaaggc ctatttaagg gcctttttct ttataaaaca agtttaaaat ggaaaggaga
    22261 aaattgtgga ggatatttta atagctacgt ttataactgc actgggagca tttttaacga
    22321 tatttgtggt gactattaaa ggatttaaag atacggatag tttacaaaaa gatcatcagt
    22381 ctattttaga tggtcaaaaa acacataccg aaagcattat tgtacacctt tcagaaggat
    22441 tcacttattt gaaagatgct atgcccacat tcacagtaaa attaacggaa atatttcatt
    22501 ctgttgagca aatatcgtca gaattgaaag agcaaagaca acaaaaagat cgtcaacttg
    22561 gctcacttaa ttttaggcaa gaaacgatac aagaatcatt ggagaatgta aatcgattaa
    22621 acgatgaaat gcaacgagtt catttggaaa atgaagaatt acgcgcacaa aattatgatt
    22681 taattcaaaa gaaaagaaat ctggaacaaa aaattgttga attaaaagaa gaaaatcgtc
    22741 aattgaatta tcaattacga cgaggaaaaa atcgttctcg tgatttagga aatgaattag
    22801 agttgtaata tttagaaaaa cgctgtgaga aatcacagtg ttttttattt ctaaaaatac
    22861 aaaatataag gggtatggtt atattatgga taaatacatt catatgaaaa atcctccaag
    22921 atttagtggg gaatggaagt ttttcgcctt tttcttttta gatctattaa cgcttggtgg
    22981 ctgctttttt tgggcgaaaa tagtcaataa tcttgtccac ttaccactct tttttgcact
    23041 cttgaattat ggattatggc ttgcgatagc gttagtgatt gtttggcgac cctataataa
    23101 ccctggtcaa cgccaaattt ttgcgattat cgaatctatt ttctttacgg ataagaacca
    23161 ctatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg atgaataaat
    23221 gcgagtcaaa ggacaaaatt cagttgataa gaaatccatt ttgcagtttg gaaaaccaga
    23281 agagcgctct atcttggact tatttccata taaatcctgt acagaagagg gagtcttaat
    23341 tactagagaa gatcattttc agcgttatta tcgtgtcgtt tccactgacg tggagggatt
    23401 aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc gtacgtatgt
    23461 gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac aaatcgtgca
    23521 aaagcggcag ttattagata aaaatcgtat ggaacagtca ctaggaagaa atctacagcg
    23581 attaagaaaa tatgaaaaga aattagtaga ggagatccag gaattaaaac gggcagaaca
    23641 agaacgtcct gacttaagct tctttttcgt tattgaagga aagacaatca aggatttaca
    23701 aactagaaat cgtcagttaa tgcgatctag tggcatttta gggttaaaac cactctacaa
    23761 aaaagaattg attaagattc tttatcgaat gaacaacatg aatgacgaat aaaaaagatg
    23821 gaggaaatga ttcatgctag atgtgataaa aacagctttt tcaacgcaaa gtaatcaaaa
    23881 ggtgattgat ccattaaaac aacttggtaa tcgtgaaata gagattttaa aatcaaaagg
    23941 ttatgattta gatttcttaa aacaagtgat gccacaaggt gggttgaatt ttgatgaaga
    24001 atatgtgacc tatggaaatg gttattcacg atgtgtgaca gtttatcatt acacaaaaaa
    24061 tcctactctt ttttggttgg ccaatctcat gaacaatcct catacagtgg ccacactaga
    24121 tgttagcaca gatgaaaagc aaaaagtgat tgatcaaatc aaccgcgcat tagatgaatt
    24181 aaatgatcga tcagataatg aacgaaaagc gactgaccga aataaaagtg cggaagaata
    24241 cctggaacta gcggaatatg cccgaaaatt gacttctaat ggggaaattt ctaaacaagt
    24301 caaagtgaga gtacatgttt acggaaatac gatccaagaa gtggatgaac gggcgcagga
    24361 actgatcgaa aatctaaaag ggatggatta tcgtgcggtg acttatctgc ataaaaccaa
    24421 acgagattgg caatcgatgt ttaccatgtt cgatgaacaa gctgaatggt ttggaaatat
    24481 caaagggcaa tcacttccat cgttaaacat tggcggagga ttcccgttcc accaccaaac
    24541 gctaaaagat ccaggaggca cacaatatgg ggaaaccatg acaggcggcc catttgtctg
    24601 gaatgccacg tataaaaata gtgtacgttt atctaccaat accttgcttc taggaatgat
    24661 gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga
    24721 ctttatttgg ggctttgaaa agggaaaaga ctttattcct ttcttaaagg aatataacgg
    24781 tattatggtg cgtttagacg gatctgacgg gatgatcaat cctttagaga tctttgccac
    24841 acggacgtat gatgaagcat ccagtttata tgacgatggt tcagtcaaag atttgaagat
    24901 caatgaagca gcgtcctatc aaacgcactt agacaaagta gtctatcagg tacagttagt
    24961 gagtcctcag ctaaaaggaa cgatgaaagc agaattcaag acctacttga atcgttttta
    25021 tgaagagtat ggaacagtac cacgcggttt tacttcctca aattcgcgtt caaatactga
    25081 tacgcaagtc acagggaaag atccagaagc ttatccgacg ttcaaagaat ttttagcctt
    25141 tttaggacaa ctggaactac caggggcttc ccaagagaaa aagaatcgga aagaagaaat
    25201 ggagtctatc gtggaatcct tatgtgagac ttatggcatg atttttgatg gtcatagtac
    25261 gattcgacac cttgaccaac aacagttggt cttttttgac attgactcta ttacaggcct
    25321 agatcgaggg gtacaacaat gtcagatgta catggccatg acgttgattt ggaatcaggc
    25381 attgattcaa ggacgaagac aacgacgctt aattaaagaa aaacaaatgg aagaacaaga
    25441 cttcaaacgc ttcttggcga tgattgatga gtgccataat gcgattaatc cagatttcat
    25501 tgaaacggta aattatgtca caaacttcca acgagaaatg cgtaaagtag aagcaatgac
    25561 tgttttagct acacaatccc cacaagaaat ggtgccagaa aatatgtctt ctgataactt
    25621 ctcgaaactg aagaaagtct ttgaattttc ttctacgaag atctttatgc gtatggatga
    25681 atctatttta cgtcatatag aaggattagt tggtaaatca atgacgaact cagaattaga
    25741 atccattcct caacaaaatc aaggagacgc tgtgatcaat tttggctcaa aagaaaccat
    25801 tcgtgttcat tttagtccga acaaacgtca attaaaattg tttgatggag gaaaataatg
    25861 gaaaaccaaa atgaaagttt aatcaaacag tatgttaagc gtcgtgcaaa gcggcgctta
    25921 tttttgtggg tgtttggtac ttctgcaggt ttgattacta tattgatcac tgtgtttgtg
    25981 accctttttc taatacttgc ggcaggttct attgataatt ctgattcaag tagtggtggc
    26041 gaagctttta caggagaata ttcggaaggc ttacccattt ataaagaaat caaaggaaga
    26101 ggcccttttt ctgatgagat tgcgcaatat gctgtaggtg cagcagtgaa gtataaatta
    26161 ttaccaagtg tgatcctttc gcaatacggt tatgaatcag cgtttggtac gtcggcgtcc
    26221 gcaagaaatg atctcaatta ttttgggatt acgtggtttg atgggtgtct gtttcctaaa
    26281 ggaactgcgc gtggaattgg tggcattgaa ggtggctggt acatgaagtt tcccaactca
    26341 aaagctgcct tctcctatta cggctttatg gtagccacac aatcaaattt taatgcttgt
    26401 gtcggaaata aaagccctgg cgcaagctta ttgatcttag gacgaggagg gtatgccgcc
    26461 gcaggaatta ctgaagacag tgcctattat aaaaattgta tgtctattat taatacaaat
    26521 aaattgatgg agtacgatga gtttgcgatt aaacactgga gagaaggcgg cggaactggt
    26581 ggtacgatta caggaagctg gggcaatcca ttcccaggaa gtagcttaga taaaaattcg
    26641 ttcagtggag gacaactttt tgggaaaaat ccaggaggag aattccgacc taacgggttc
    26701 catgatggac tagactttgg ttcagtcgat catccaggaa gtgagattca tgcggttcat
    26761 ggcggaaaag tcgtgtacgt tggcaatcct ggtatttcag gtttgggtgc gtgtgtgatc
    26821 gtgattaatt acgatggctt gaatatggtg tatcaagaat ttgcaaatag tacaggaaat
    26881 tctcgagtga aagtaggcga tcaagtgaaa gttggtcaag taattgggat acgagataca
    26941 gcacacttgc atttaggatt tactcgaatg gattggcgtc aagcacaagg acatgcattc
    27001 attgatgatg gtacatggat cgatccgtta ccatttttga atagcagcaa aaaatagatc
    27061 aggagaaaaa atatggaaaa taaaacctta aaaatcgtct taacagctgc aattagcgtt
    27121 agtttaatcg gcaatattgc gttgttttgg cagcttaaag aagaacaaga gacaaatacc
    27181 tataaaattg aagaagtggc taaaaacgcg tctgataagc tcaaaaaaaa ttcttctgaa
    27241 gcacaggcga agattgacaa gttaaatcaa accatcaatg agaaaaacca agaaatcgat
    27301 cagctgaagc aacaagatgg cacaaaagca aaagaggaag taactgaagc acaaagaaag
    27361 acggcagaag agttcggtca attggccatt gataaatcat taagcagaaa tgaattaacg
    27421 gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca aattaagtcc tgtagacgat
    27481 gatcaccaac atgatgatta cggaagttac tcttttacat taggtgacgt gcaaacttac
    27541 gctcaaacaa gcagtgtaaa agaggaacaa aattttgtcg tatttgttga ttatacaatc
    27601 ggcaatcctc aattcaaaga tgtcaaacca caaaaaataa aaggtggatt aactgtcact
    27661 gaaaaacaag tagatggtca atggaaagtc acagacttta gttatttcac tcgttaggag
    27721 gaagaaaatg agtaagaaaa aaggagcaag acaacgtttt aaaaaacgaa aaagttatct
    27781 tagatggctg gggatcttta tgattctcgg ctttttgatt cttgctagtc cgatttacac
    27841 acataaaaag gtagacatac cagatgaagt aagcacgaac gcatttttaa acacgaagga
    27901 actgacgatg gtttcaaaag aaaaaggagc gaaccagcaa ttagtttttg aatttgcgat
    27961 cgacgatgaa gatcagagct tattaaaaga actagctaat cttaattatc gtgtagaagt
    28021 aaagacagct aaaggggatt atcaagcaat aaaagccaaa gtgatcaagg tgtctgatga
    28081 ttattttgta gtgaagttag cacatgtacc agaagaatat attgctatgc gattaactat
    28141 tattcctgaa aaaattgatc caaaggtgga tatgcaagaa ccgcaagatc tgatttttta
    28201 tattcacgaa gataaggtca aagatcacgt gaaagacgaa gattatgaac aacatgcgat
    28261 caattacaaa gtgaaaggat acaagaaaga acaaaaagca gcacaaaaac aaattgaatc
    28321 cttccaagca accattgatt tgaatgaaga attagtgaag aaactaaaag atcaattgcc
    28381 ttatcaagtc gcagaagatc aggaaaatac tgaaaataag attaatagtt accagcaaga
    28441 aattgaaaca tcgaaaacgc aaatgaaaga tcaagaagag agcattcaaa aacttcaaga
    28501 aaaaattgat cgtatgacaa aagaaaatat ttagaagaat aggtggagta aaagaaatga
    28561 agcgagcgaa cttggtcggc aaagatactt tcgctgagca gaaagtattg attaaagcct
    28621 atctcaaagt tgtaaaagct aaagaaatat gtcaagaagt gaattatcta acacaacaat
    28681 atctgagttg aagaatggta gaaagagttt agatacctta aaattcagat catttgaagc
    28741 tctatatata gctgctaaaa aatattttac agctgataat aaataaaaaa ttaaggtggg
    28801 ttctatgtga aaaaaacgta tcaaaagttt ttatggatta gctatatcgt cctatttatc
    28861 gttgggtttc tcgtcagtgt gtccgtgatc gtcggaacgc tttttctttt gttctctgac
    28921 gaagatctgg tacttaaggg gttaatgagt acgttatcgt gtgtcatgtt actttattta
    28981 tgtatacaga tcaacgaaat tatgccccct cattggcaag aaaagaagaa agaatttaag
    29041 gaagaaatcg aaataaaatc caatgactgg attgaataca catggcttcc tctgaaaaga
    29101 aatagcaaac gaaaggaatt ttgggcattg caattatggc caataggaaa actttttatt
    29161 tatatggctt cacttatggt tactttaatc ggttatttgg tggtggtcta tggggtggcc
    29221 tattacttgt taagtgagcc aaaacagatc gcggcgtcaa atattttcac ggcagtaatg
    29281 acattaatgt gtgtcatgtg cttttctatt tttagttact tactcgtcca atttatcagg
    29341 caaaatttaa caatgagtaa acttttcaaa agtttattgg acttacttat cttaggcagt
    29401 atgttaacga ctctaacaga tcggttcgaa caatctgaaa aaacggtaga ggggataaag
    29461 aacttcttaa cgacgattga tcaatttata aatggatata tgtatttgtt tgaagtaggc
    29521 tttattctca ctttgtttgg ttgtgtattt ataaaactca ttaaaagatc aatcgaaaaa
    29581 gaaatggtta ttgatctaat tccgacgctg agcggctatg agtattacgt tgattttgga
    29641 aagtatgagg agttgatcca tacggatatc gttcacacga gtatccatga agtaaccgtt
    29701 aagggcatca gatctacttt aaaagatgga caggagacga aaacaatcga ccgaagaaaa
    29761 gaggtctaca atttttataa agttgaagca agtctacgat atggcattgg gaaattgagg
    29821 ttagatggga gtaatattca acaaaacaaa ccatatgttt taggaaaagt ggtgatttag
    29881 aacttcctat gaaagatgaa taaataagga gaatcttttg aaaatgattc ttctttacag
    29941 aattgtgctt atagttggca cggagttaaa aatcttaaag aaggagcagt tattcaaagt
    30001 gaaagaacat cataatcagt tactatgtat taaaacgggt acgatgaaca ttcaataccg
    30061 aacagaatat ttagaattaa cgaatcaaac tgattttttg tcgattcgag agcaacataa
    30121 gcaagaaaaa ttaatggcta cttatagcca attgattgat gagtgggaac aattcaagga
    30181 atctaaaaca taaaattcca tttaaacacc ttatttttta aagagcataa ggaaacgtgt
    30241 acccactttc aaaataaagt gggtagatag gctgtgccta tcacgtttct gaaaaaaata
    30301 aatgacccca aaattttcaa tttatgtgta ggtttatggg tagcaaaaat atgaatgtgg
    30361 gcagcaaaaa gttcatttgt gggtagtaaa attcaaatgt gtgtagcaat gtgtgtagct
    30421 acacacattg ctacacacgc tataacatat agtgaaaagg agatatttat ggaagtaaaa
    30481 ataagagata ttgatgaaaa aacgattcaa agaattgacc gaagatgtat tgaattaagt
    30541 gaacgcaccg ggactaaatg gagccgaaat cgatacctaa aagttttgat tgaaaatgat
    30601 tttgaccatg aacttatgga tcatagaaaa gatcaatttg atcggttgtt agaaaaattt
    30661 gtggatattc aaacttataa tacgaaaatt ttagaagaat atattgcaac aaataatcaa
    30721 ttaatcgcct tgttaattga ataataaaaa tagtattgtg aatttataaa aggaggaaat
    30781 caattttggc aaaagcaaca agaaaacatg tatatcttta cgatatgcat atcgaagtac
    30841 ttgatgaaat aatttctact catgaagata tatctaatta tagtgaagca gtcagacatt
    30901 tgtgtatgat gtatgatcca aaaatcgaag aaaaaataaa tccaattcaa attaaattga
    30961 atgcaatggg aaaagaaatt tctatattaa ccgaaatggt ttcagagttt gcggatcttc
    31021 atttgaaaga tacaggaata ctaattggta caaattcaac tgtctatcaa gaagcaaaag
    31081 agcgtgtgga agggaaaata aaagcgaatc aaacgaaaaa gtattctcct aagatcaaga
    31141 aaccaataaa tgaatctgtt catgccagag tagacccgtt tgatccaaca aagtttttaa
    31201 aaagataaaa agtagaaggg gaacatgatg aataaatcgc ctgcagtcat tctcacttgt
    31261 caatttacct tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa
    31321 aaagcgctgg aagaaaagga acgacgcacg ccggacgaag aacaggaact gcaacgaatc
    31381 aagaatgctt tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagaca
    31441 gataaagaat tcagtgaaac gtcgaatgaa gctcaatcgt tgatggaaca tggaatcaat
    31501 tttgacaagt tagaggaaca agattttacc aaatatcttt cttatatgac ccgtcactat
    31561 gcgttagaac aaaagaaaga actgacgcaa agggaacaaa aagaatacca acggttgaac
    31621 caagcaatga aaaaatatca aacagagcca cataaaaaag cttcaggaaa tcttcctgga
    31681 gttttttcta tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caaacaaatc
    31741 ttccaaagag ggcaacaaaa aggctcaatc atttatcaag atgttgtctc tcacgacaat
    31801 tcgtatcttg aaaaaatggg cttgtacgat cccaaaacag ataccttaga tgaagaagga
    31861 ttaaaagcag ctggaagaaa aatgatggag acattattcg agaaagaaca attgaatgaa
    31921 acaggttact ggatggcaac gattcaccgt aacactaagc atatccatat tcattttgca
    31981 gttgttgaaa aagaaaatac gcgtaaacct tacacggagg ttgaaaatgg tgtgagttat
    32041 atcgtaccga aaggtaagag aagacaagca acattagacg caatgaagac caattatgta
    32101 catgagttag aacgtttttc ttatgaaaag gatcgaatga ttggtatgga gaaacgaaat
    32161 ttattgacgc ggaaaagtga tttgcgtaac accttaacaa aagtggtaaa agagccagcg
    32221 cgttacgatc gacacgcaat gaacttatta aatgaagtat ataaaaccct acctgacaaa
    32281 cgttcggaat ggcattatgg ggatgaaaaa cgaaccaaac tttcaccata cacaagagag
    32341 aaactcgatg agttaaccaa atatgtgtta acaaaagaac ctgaatatca agaatatata
    32401 cagatttcaa aatcattgaa agaagaagca aaaagtcttt atggggaaag caagagagaa
    32461 tcaaaggatg cagaaaaaaa tgctctcttt gatttgaaga agcgcacagg aaatgccatt
    32521 ctaacagaac tgaagaaaca ggatagtcaa ataaaacctt taatagaaag tatggatcag
    32581 atgcaatcta tttacaatga acgatccttt cgaacgctta aacagttacc aacgaatgaa
    32641 tacaaatata gttacgaaga attcatgaag cgcaggaaac aattccaaca aagagttgtc
    32701 acgcaacgga gtttgcgtca attaaatcga acgattaatg cccatgaaga aaaataccac
    32761 gcaagtaaag cctacgaaga gcaacaacga agaattcaaa tggaacaaga aagacagtaa
    32821 agacagtagg aggaatagca atgaaacaat atttagatca atggaaagtt atagaaggat
    32881 cattaagaga agaacgaatc gaacaacttc ccgattgttt agaaaaagag catttgtttc
    32941 agataagaga aatgttgaga aacgaacagt ttgatccaaa tcaattttta gtcgttgagt
    33001 actcagctac tggtgtgtac tgttgtaatc atgtgaaggg tgaaaaatat tttatcattc
    33061 aagaatatga gggcaagtta gcgccttatt atactacttg ggaaatgaat gaagagggga
    33121 tcaataactt cccgtgtaaa agcattgaag agtctatttc actcacagaa tgttagttgt
    33181 taagtgaata aaagaagtaa aagagtcctg aacgtaattg tccaggactc tttttttcaa
    33241 tgaattgttt tccgccaatt caatgagtgg tgcgatttgc gactacatta tatcaaaaga
    33301 aagagaaggg aggaagaaga aatggcttat tcaacgattg gagttgttgt tgaaaaaagc
    33361 agagacaatc tcgtttttgt gacagaaatt caaacgggca gagcttttgt cgtcacagac
    33421 aaagcggcaa aagcgtatca aagtggcgat attttaacgt taaatctgac tacgaagacg
    33481 ttcgtcgatg cggcagaaga ttatccatt
//
LOCUS       DACEJR010000026        33259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEJR010000026 DACEJR010000000
VERSION     DACEJR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.026"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..226
                     /locus_tag="IW651_001259"
     CDS             <1..226
                     /locus_tag="IW651_001259"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0813999.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IW651_001260"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IW651_001260"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IW651_001261"
     CDS             533..1336
                     /locus_tag="IW651_001261"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0814000.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="IW651_001262"
     CDS             complement(1585..2649)
                     /locus_tag="IW651_001262"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0814001.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="IW651_001263"
     CDS             complement(2646..3731)
                     /locus_tag="IW651_001263"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0814002.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IW651_001264"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IW651_001264"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0814003.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IW651_001265"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IW651_001265"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0814004.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5980..6633
                     /locus_tag="IW651_001266"
     CDS             5980..6633
                     /locus_tag="IW651_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0814005.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            6718..7623
                     /locus_tag="IW651_001267"
     CDS             6718..7623
                     /locus_tag="IW651_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0814006.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="IW651_001268"
     CDS             complement(7625..8257)
                     /locus_tag="IW651_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0814007.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="IW651_001269"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="IW651_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0814008.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="IW651_001270"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="IW651_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0814009.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="IW651_001271"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="IW651_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0814010.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10137..11375
                     /locus_tag="IW651_001272"
     CDS             10137..11375
                     /locus_tag="IW651_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0814011.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11423..13552
                     /locus_tag="IW651_001273"
     CDS             11423..13552
                     /locus_tag="IW651_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0814012.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13527..14216
                     /locus_tag="IW651_001274"
     CDS             13527..14216
                     /locus_tag="IW651_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814013.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14624..15310)
                     /locus_tag="IW651_001275"
     CDS             complement(14624..15310)
                     /locus_tag="IW651_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0814014.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15446..15640)
                     /locus_tag="IW651_001276"
     CDS             complement(15446..15640)
                     /locus_tag="IW651_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814015.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15690..16130)
                     /locus_tag="IW651_001277"
     CDS             complement(15690..16130)
                     /locus_tag="IW651_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0814016.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16134..16979)
                     /locus_tag="IW651_001278"
     CDS             complement(16134..16979)
                     /locus_tag="IW651_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0814017.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17128..18546
                     /gene="pepV"
                     /locus_tag="IW651_001279"
     CDS             17128..18546
                     /gene="pepV"
                     /locus_tag="IW651_001279"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0814018.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18622..19824)
                     /locus_tag="IW651_001280"
     CDS             complement(18622..19824)
                     /locus_tag="IW651_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814019.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19811..21598)
                     /locus_tag="IW651_001281"
     CDS             complement(19811..21598)
                     /locus_tag="IW651_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814020.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21611..22564)
                     /locus_tag="IW651_001282"
     CDS             complement(21611..22564)
                     /locus_tag="IW651_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0814021.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22749..23099)
                     /locus_tag="IW651_001283"
     CDS             complement(22749..23099)
                     /locus_tag="IW651_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0814022.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23299..24378
                     /gene="pepA"
                     /locus_tag="IW651_001284"
     CDS             23299..24378
                     /gene="pepA"
                     /locus_tag="IW651_001284"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0814023.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24521..24841
                     /locus_tag="IW651_001285"
     CDS             24521..24841
                     /locus_tag="IW651_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0814024.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24863..25327
                     /locus_tag="IW651_001286"
     CDS             24863..25327
                     /locus_tag="IW651_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0814025.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25523..26128
                     /locus_tag="IW651_001287"
     CDS             25523..26128
                     /locus_tag="IW651_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0814026.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26167..27456)
                     /locus_tag="IW651_001288"
     CDS             complement(26167..27456)
                     /locus_tag="IW651_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0814027.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27884..28363
                     /gene="rlmH"
                     /locus_tag="IW651_001289"
     CDS             27884..28363
                     /gene="rlmH"
                     /locus_tag="IW651_001289"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0814028.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28716..29030
                     /locus_tag="IW651_001290"
     CDS             28716..29030
                     /locus_tag="IW651_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0814029.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29046..29432
                     /locus_tag="IW651_001291"
     CDS             29046..29432
                     /locus_tag="IW651_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0814030.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29461..30588
                     /locus_tag="IW651_001292"
                     /pseudo
     CDS             29461..30588
                     /locus_tag="IW651_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            31321..33180
                     /gene="ltrA"
                     /locus_tag="IW651_001293"
     CDS             31321..33180
                     /gene="ltrA"
                     /locus_tag="IW651_001293"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0814031.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT        10717 a         6146 c         6751 g         9645 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcaa gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
    10561 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
    10621 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
    10681 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
    10741 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
    10801 gggtcgttct tttagatctt caaatcatct taggttttat tctagcaggt atctgggcat
    10861 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
    10921 tatttacaga tggcgcagtc gatgttgcac caatggttgg tttcttgatg accttagcga
    10981 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
    11041 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
    11101 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtgttagctg
    11161 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaattaca acgattgctc
    11221 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
    11281 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcggt gcgatcttat
    11341 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
    11401 tagataaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
    11461 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
    11521 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
    11581 aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
    11641 cacacaagcg actaatgcac tgattgaagg agatgtagca aaagtcggcg taatcgggat
    11701 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
    11761 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aaaacttaaa
    11821 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
    11881 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
    11941 atctgtgaaa gcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
    12001 aagaagaact cggacagcag caatcaacgc atctatttta ccaaaaatgc tagatacggc
    12061 aacctcgaca gaagactccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
    12121 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
    12181 ttccggtcct gctgcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
    12241 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
    12301 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
    12361 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
    12421 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
    12481 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
    12541 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgttct
    12601 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
    12661 acaggcatta gcagactatt gtcatacgac ggttgaagac atcgcagagc agatcatgga
    12721 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
    12781 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
    12841 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
    12901 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
    12961 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
    13021 accagaatcg atcgaagtcc acgtagaaat cgatccacat acttcaaaag taacggctat
    13081 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
    13141 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
    13201 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
    13261 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
    13321 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
    13381 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
    13441 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
    13501 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
    13561 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
    13621 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
    13681 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
    13741 gagaaatcta tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
    13801 aacgaatcac ttttgcaact tatgcagaag atgaagggat ccgattgatg acggagccgt
    13861 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
    13921 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
    13981 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
    14041 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
    14101 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
    14161 aagaaataaa agaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
    14221 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
    14281 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
    14341 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaaaggtg ttcaaaagct
    14401 tgcatctgcg gtaataagaa cagccaagtt aaaaacataa gaagctattt ttacttggct
    14461 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
    14521 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14581 tatttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
    14641 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
    14701 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
    14761 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
    14821 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
    14881 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    14941 cttttttatt ttgagcgatc actgcctgat cggtttgttc tagtattttt tctagtttag
    15001 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    15061 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    15121 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    15181 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    15241 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    15301 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    15361 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    15421 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
    15481 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    15541 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    15601 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    15661 atacaagaaa aatgcttaga cggtatttat taagaattta catcatccaa agaagcgtac
    15721 gcttggaata actgttctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    15781 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    15841 cccacaattt cgtagctaaa atccaaccgt gttcccgtat acttttggat agttgggtgg
    15901 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    15961 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    16021 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    16081 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    16141 tcgttttcca ttgatttttt acaaaaataa ttcatccact tcggaagtgc ttcgctgatt
    16201 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    16261 taaacagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    16321 atcccagcta aagtgtcacc catgccgcct gttgccatag cagcagaacc taaaggattg
    16381 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    16441 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    16501 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    16561 aaaagatgat tagcaacaag taaacttaaa gcagagccat caatcaccag ccattgttct
    16621 tttgattgtt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    16681 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    16741 gtccaatcta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    16801 tcagttacta cagtcgtgag acccgctcca ctgtgtacgg cagctaaagc actcataatg
    16861 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    16921 tgtgaaatct ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    16981 gaaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    17041 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    17101 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    17161 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    17221 gattcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    17281 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    17341 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    17401 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    17461 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    17521 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    17581 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    17641 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    17701 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    17761 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    17821 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    17881 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    17941 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    18001 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    18061 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    18121 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    18181 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    18241 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    18301 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    18361 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    18421 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    18481 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    18541 aaataaaaat actaaaaaac tccggctatc tctgacagct ggagttttta tgtttaaaaa
    18601 tgaattagta catcggtatt cttaaaagta atttagttcg tcttttgatt tctcaggagg
    18661 gatcaaaaag gcgaaagcac taagtatagc aaaacttgtg atgatttgcg tacgatacgt
    18721 aaaccaataa tgaatctgtg agtgattagc taaaatattg taccaaatat acggcgttac
    18781 agcagctaaa acaaacaaaa ctagaagttg gaacggacgc cagctatttc gtttacgata
    18841 agcaaaatag agaaaaacaa gacaagtaat caatagcaat aatattgcta cttttggata
    18901 catcaatcta atattgcttt tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc
    18961 ttccgttcga aagaggattt ggttgattgc atcttttaca atgtttttgt tcaatatgat
    19021 cgatgatagg atccatttag ccacccatgt caaaccatag cctaatcccc aaaagatacc
    19081 ggtaaaaaca aaactgccta cttgtttgct caatgaactg cgtttatcca ttaaatactt
    19141 atttgttaca taataaataa tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa
    19201 caagtcaaaa aagttagcta aagaaccaat gacaaagaaa tacaagaata attgttggtt
    19261 ggtgttggag agttgtggtt ttgttaaaaa tagtaataca gacaataaag tcaaaatcca
    19321 aatatttgaa aattgcatac tgaagaaaaa ggttgcagca tttcctatag caagcgaaat
    19381 caacaaggct gtcgttaaat agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc
    19441 aatcagcaga aaaagaatgg ctgcgtaaat cattcgaata ctccccaaac tcatccctag
    19501 cagtaaaggt cgtaaaaaaa gctgataacc atgccaataa cggggataat ccgccataga
    19561 catcgcgttc tccaaggagt tcaattcagg atctgctttt gtttttcgta tcatcaaatg
    19621 atcagtaaaa ttatctaacc gagtgcctag catatttcct tgattgatag aaggatacat
    19681 tccttctttt tcaaccatct gtagagattt ttctgcattc gattcaatag agtgctgcgg
    19741 aatgaaaaaa gtaattgtca gtagtacgca atagctagcg atcaagagaa caaaagcaaa
    19801 aaatatcttt ttaaatagtt tcatgagtat ctcctatatc tgttttcatt ttttctcttt
    19861 tatctagtaa aaaaggagag aggatataaa caggtagacc taatgctaaa acaaacacat
    19921 accgtaaact aaaagccaca ggagtagcaa tcataatagt agcaaaaaca agcataccgg
    19981 gaagaaaaag gagtaaatac cgatattttt tcttaaagat tgctaaaagt gcactgccga
    20041 tagctaacca cagtaatgtg ccggaaccaa agaaatcacg agcatcgatg atttttttca
    20101 ttcctgacaa tccagtaaga tgttcaatca aatctacttg cttcatacca taattattcg
    20161 tgtttacacg agtatctaaa aagccgtaac tattttttgt accaggtacc cagaacccga
    20221 acgtttctaa aatataagca tctgtataga tttttatatt acttggtaaa tttcttgccc
    20281 atagtgatag aaattctttt ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc
    20341 acttgattga gtctgcaaga ctaggagaat acgcttcata atcttttatc gggagtagct
    20401 gattcatgaa tgctttatct ttcttagaca agtcaccatc cataacaata gttctagaaa
    20461 tttgctgcaa aggaacacct aattgttcag cagtttctgt aggaatagca taacgagcca
    20521 tcaacgggtg gatgagtagt ccagcaatca ctgtataacc cagcaccact tgaaagaatg
    20581 cttgatacct tttttgtttc aaaataatca agccgcaaat caaacctgtg ccggttacta
    20641 cataaatccc attatttctt agataggtgg tcagtaaagc aaggatgatt acgtaaatct
    20701 gattgagaat cgttggcttt tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata
    20761 aaaagcaagc gatagagaac aaagtatctt tttgcatgtt caaggcgtag attggaaaaa
    20821 ccggtgcaaa tgcaaaatag cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt
    20881 ttaacagcca gcaaagagaa taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg
    20941 aaaataaaaa tacaccgtga ttgatcgtat cagttggtaa atcatgaaca aatagcttga
    21001 ccagtaaggt gaataagact ggatgatgat tgcttatagg aacatcaccc atagattggg
    21061 cgaccgaact taacgaatca ggcaaaacca ctccaggata caatgccaac agataaggaa
    21121 gccaacacag aactaaaaag acagtaaaga ataaaaaata ttttcttgaa gagaaagcct
    21181 gtgtgttttg agcagtaaaa aatgtttcca gttttggcat tgataaataa atcgattgta
    21241 aaaataaaaa agaatacaca aaaatggaga aataaacaag aaagtaaaaa atacttccat
    21301 cagcaatcgt tgtctttgta tagtttgctg agacatactg aaattgtatc tgtctgccaa
    21361 tagaaagtgt aagagcaaaa gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt
    21421 tttttattga aaacaactct attccatatt gcttaaaagc aagtagcaaa accatggcta
    21481 aaccaatgat tactgttgga ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac
    21541 ctgacgcaaa taaagttgca aaaaaagtag gaaatagatt ctcctttttc ttatgcataa
    21601 aaaaggcctc ctacttcatt tctttatttt gatattcgtg gtagacatgg tataaattca
    21661 attcatattc tttcttggca tttgtgacga ccgtatccaa aatcaaccct gttactaatg
    21721 aaagaagggc taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa
    21781 agcttgtcag catatattca cgaatcacag gaatccccat gaccaatcca aataaaaaga
    21841 agaagatggt ccagatccca aagaaaagca aaggtttata atctttgcac attttgatga
    21901 tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctaccttcag
    21961 gacgatcgcg ataatcgatt ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa
    22021 tcgtcaattc tgtttcgatt tgaaaaccag aactcataat agggaagctt ttgacaaaaa
    22081 ttcgattgaa tcctcgatag ccggtcatga catcgcgaat ttttgtttta tataagtaag
    22141 taattgtatt tttaaccaga ttattcccaa aatcatggaa aggacgctta ttttcatcaa
    22201 aataggttcc atttgataga cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt
    22261 tctcaagcaa accatgtgca gcttctgcag gataagtatc atcaccatct accatcaaat
    22321 aataatcggc atcaatatca aaaaacattt gacggattac attccctttt ccttgacgtg
    22381 gttctttctt tacgattgct ccgccttgaa cggccaattc gtatgttcga tcggtcgaat
    22441 tattatcata gacataaata tctgcttctg gtaattccct tttaaaatct tcaataacag
    22501 ttgaaattgt cgcctcttcg ttataacaag gaatgagcac ggcaattttt tcacgtgatt
    22561 ccatttgtca ctctccttat ataataaaac taatcttatt tcaataatac cgtataataa
    22621 ctacaaatac tagctattcc aagaggaaat agccctattt ttataaaaat cacccactat
    22681 aaaagaagga taggatacct ctcttttaga tagtgggtgc gtattcgtta tttcattttt
    22741 ttgacgtatt aatctattgc gccctttttt cttgaaatgt ctaatacggt tcctgtatga
    22801 gcatctgcta aaaattcata ataaacagtt tcgccatctt ctatccggga aagcccaccc
    22861 cgataagcac tcgaccgaac ggcgaatttc cgaataggtt ctttttcaaa agagatccaa
    22921 gcaccttcga ttgcgccttc ttttaaaaaa gatgttttga tattttcaag cacttggtct
    22981 gctgataaca cttttttgcg atgataccaa atcgttgaag ctaccccact caataatcct
    23041 agaccgatcc caacggctaa cccgccttta aaatagctta attcgctttc ttcatacatg
    23101 tggatatacc tccttcttct cacttgctac tctttgtgtt tattttacca cactctcata
    23161 ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat acgatcggtc
    23221 tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag tttagtctga
    23281 aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa
    23341 ttacaaggaa caagcggttt tgaacaagac gttcgcgcct atatggaaag tcatatggaa
    23401 ccgctggtag atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat
    23461 gaagaagcgg atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg
    23521 ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct
    23581 tacgttgttt ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt
    23641 atttcttctt ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa
    23701 gtaacagata ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt
    23761 gttcttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag
    23821 aatatcatca gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta
    23881 gaagcgttac aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa
    23941 gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt
    24001 gcagtagact gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta
    24061 ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt
    24121 gagtacttgc tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa
    24181 ggcggtacag atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt
    24241 ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa
    24301 gctgcacgag aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg
    24361 atcgtatacg ggaaataacg cgatagtgaa actataaaat ctggcaaaag caaccttttg
    24421 tcagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    24481 ttaattgaaa aatacataaa cagaaagtag aggaaaaaga atgattatcc caaatagttt
    24541 agaagaatta gcaggctacg tagaaactgg aaaaagtgta tttttcttta ctgccggctg
    24601 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa atcgaaaatg acttttctga
    24661 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    24721 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    24781 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa taaaaggctg
    24841 acatctgtta ggaggggtca agatgaatca agaatataag aacattttag taggtattga
    24901 cggtagtgaa caagcacttg ctgctttcaa aaaagcgtta gaagtggctc gccgtaataa
    24961 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataatttta tgggctatgc
    25021 tcctattgat caaaatctag tcgatcagca aactgaaaca gccaagcaaa tgatcgaaga
    25081 atgtaaagaa tatggaaaat ctgttgatta cacaaatata gaaggaatta ttgcatacgg
    25141 ctctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgcaatcg atctaatcat
    25201 ggttgggcag tctggactaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    25261 cgtcatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    25321 taattaagca agtggaactt gtataacctt tttaatcaaa cagtaaaggg actgtgacaa
    25381 aagtcttgtc acagtccctt gttacgaata aatggtgttc cagaagaagc ttcttcggaa
    25441 ataaagcatg aaaaaaatct catgcagtga atgcaatttt ttgttaagat aaatctcagc
    25501 aaaggataat ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac
    25561 tttgatggtt atcatagccg acgataaagg aacagaaaac actgtcgaac gtaaatggaa
    25621 tgttgctcgg atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc
    25681 cgatcatcta acaattgaag ggaacggcca agtaagctta acagatgaag aattagcgaa
    25741 attgaatcga ttgattaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc
    25801 gaagatcgtt gtcgggtatg taaaaacatg cgtcccgcat cctgactctg accatttatc
    25861 gattactgag acagaagtgg acaatgggac agtattgcaa attgtttgcg gtgcaccaaa
    25921 tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga gccatgatgc cagatggaat
    25981 gatgatctgg ccaggaactt tgcgaggggt agccagctat ggaatgattt gttctgctcg
    26041 tgaattgcat ttgccaaatg ctccagaaaa aaaaggaatc ttagtattgc ctgaggatgc
    26101 cgtagtcgga gaagtatttc caaaataaag aggttgtgac aagtctttgt cacaacctct
    26161 ttattattaa ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc
    26221 aacagtagtt gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac
    26281 ttttttctta tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat
    26341 cttagtaatg acgtcgtatt gtttcaaccc agctttttct gccggtgtag ctgtttggac
    26401 atttgtcacg atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg
    26461 ctgagtagaa acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt
    26521 tccgtctttt tccagttggt tgatgatatt cactacatcg ttacttggga tggcaaatcc
    26581 cattccttct acgcttacat tagaagtcga ttcagaagta cttgcgatct tacttgagtt
    26641 gataccgata acttgtcctt cgatattcac taaaggacca ccagaatttc ccgggttgat
    26701 cgctgcatct gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt
    26761 tacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca gaattcgcat attcagatcc
    26821 taatggggaa ccaatcgcaa tcgcaggttc accaaccttc aaagcacttg agtccccaaa
    26881 agaggcaacc gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataggc
    26941 atctgttcca actaattcag cttttacctt cgtaccgtct ttcatcatga cttccaaacc
    27001 ttgctgtcca tctactacgt gattgtttgt gactacataa gcagtattcc cgtctttttt
    27061 gtagattacg ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact
    27121 ttcttcttgc tgcccgaata atccgccaaa accgcttgat tggttttgac tttgtaagtt
    27181 gataacagaa acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac
    27241 attgactttg acattttcca caactgtttc accggcagaa ttttgattgc cgcttgtagc
    27301 agtagaacta ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc
    27361 tccaacgatc cctccaacaa gccccagacc caacttacgc cagaggctat tatttttatt
    27421 ttttttcatc tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca
    27481 tttatctact gttagtatag tcttatttgt tgactttagt ctactttgag actttgaact
    27541 ttttatgaaa aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt
    27601 tccacagata ttgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat
    27661 ccacagcctg tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt
    27721 gatgaaataa ggtaaaacaa cgctgttttc tgtggataaa cctgtggatg ttgtgcataa
    27781 ctgttgggga aatcttttta acaaaaatag tcaaaataaa catatagacg aaaaaattga
    27841 ttataataaa caaagtgagc gtgaagatca aaggagagaa aacatgaata tcaaaattat
    27901 ttcagtagga aaattaaaag aaaaatactt gatccaaggt atcaatgagt atgtcaaacg
    27961 gctgaatgcc tatgcgaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa
    28021 tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa ggagaacgga tcttagcgaa
    28081 aatcaaagaa caagaatacg tctatggctt agcaattgaa gggaaaaatc ccacaagcga
    28141 atcttttgct aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt
    28201 tatcggcggt tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc
    28261 ttttggcaaa atgacgtatc cacatcaatt gatgcgcctg atcctcgtcg agcaaatcta
    28321 ccgtgccttc cggatcaacg cgggggctcc ataccacaaa taatgatatt ttttaagaga
    28381 aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg
    28441 tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt
    28501 tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta
    28561 gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat
    28621 gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc
    28681 attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca
    28741 acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa
    28801 gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa
    28861 gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg
    28921 aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc
    28981 gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag
    29041 gaaaaatgat gagattagca aatggcattg tattagataa agacacgact tttggagaat
    29101 tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag
    29161 atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag
    29221 taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta
    29281 tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt
    29341 atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac
    29401 aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc
    29461 atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg
    29521 atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc
    29581 cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga cattccctat
    29641 ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa
    29701 cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata
    29761 cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt
    29821 gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat
    29881 ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac
    29941 ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat
    30001 tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa
    30061 gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag
    30121 ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca
    30181 ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct
    30241 gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt
    30301 tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg
    30361 aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc
    30421 tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg
    30481 aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct
    30541 tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatcagtg tgcgacatgt
    30601 ttctaactga aaaggttagg cacttaaaag aagaaaacat aagtttcctt ttaaggagaa
    30661 ctgatatttt gagcagtgga atgatggggt aacgccctga aacgctccct taatactccg
    30721 actggctgtt taacatatag ctgttagatg gtcagaagct cggtgaagtc ggctgaaagt
    30781 taccgtaaat atgataaatt catatacgaa agttgttcag aggtgaatga tgtaacctat
    30841 aggtcggagg tctataaaat actcacggtg agaatgtaag taatactact gacgaacttg
    30901 cgaatgaaag aatctataca cggactgtat agaaatgtac aggattctac cgtatcaacg
    30961 gaacagaatg tatgttgtgg tttagtaaga ttattggtta tgaacaacca tgcaatgtta
    31021 caggcattgg aaatatacag gttcaaagca agcacctaaa agtatatata aagataaaaa
    31081 tatcgaaaca tgggaagctg gcaatgtgga ggaaactata ttccattgaa acatttgtga
    31141 cgaagaataa taagcacatt tatgccagtg aaagtggtgg cacagtacca atgaggatat
    31201 agaaatatat cggaggaata gccactagtc aaaagaaatc gttaattaaa tgatgtattt
    31261 catagaatcg agtaagacaa aataaacact acaacctaag taaacaaagg tagtggtgct
    31321 atgaataaag taaaaacaaa taaaacactt cgtcatagtg aatactatgg catgacacaa
    31381 atatttgatg aactgtatgt aaaaagtgat aaaaaagaag tttttacaga gctcatgaca
    31441 attattatga gtgatgaaaa tattcgacta gcatacagaa atatcaagaa taacaagggc
    31501 agttatacag cgagtaccga taagaaaagt attcgagaca ttaaaacact gtcagaagat
    31561 acctttctaa ctgaggttaa agttcttttt cgctgttaca aaccacgagc agttagacgt
    31621 gtagaaattc ccaaaccaaa tggaaaaaca cgccctctgg gcattccatc aatctgggat
    31681 aggttaatac aacagtgtat tttacaagtc ttagaaccga tatgcgaagc taaatttcac
    31741 aattatagtt atggatttcg accaaaccgt tccacacatc atgcgattgc tacctgcaat
    31801 cgactggcaa atagggggaa gctacacttt gtagtagatg ttgatattaa aggattcttt
    31861 gataatgtca atcactctaa acttttgcgt caaatgtgga cgattggaat ccgtgataaa
    31921 tggctattag gtattattcg atccatgcta aatgctccta tagtcctacc aaccggtaaa
    31981 gtcgaacacc ctaaaaaagg tgtacctcaa ggtggtgtcc tatccccttt actatctaat
    32041 atcatattga atgaattaga ttggtgggtt gcttcacaat ggcaaaatac cacgattgag
    32101 acagaaaaga ttatggatag gcgagataaa ggcaaaggca ttgataagac taatcagtat
    32161 cgggcattaa gacgaacaaa cttaaaagaa atatacattg ttcggtatgc tgacgacttc
    32221 aaaatctttt gtagaaaacg aagtcatgca gataaaatat ttcacgcaac gaaaatgtgg
    32281 cttaaagaac ggttatcact tgaaattagc gaagaaaaat ccaaaattgt caatctcaaa
    32341 aaaggtaaat cagaatatct aggatttgaa ttaaagctaa tcaagaaagg taaaaaatac
    32401 gtagtcgaaa gtcatatgag caaaaaatca atgacaagag ttaaaatcca attaaagaaa
    32461 caattacgta aagttgccag accgaagaat cattgcgaac aagcaaaaga aattggatta
    32521 tataattcta tggtgattgg aatccatcaa tattatggaa ttgcaacctg tgtaaatctc
    32581 gattgcagta aaatagccta tacactaaaa ccgttcatca atcacaaact gccgacaaag
    32641 aaacatggaa agatattaaa tacatttatc aaatcgaagt atggaaaaag taaagaaatg
    32701 cgatggttga ataatgtgcc aattgtaccg ctcaactatg ttcagtttag acttgctgtc
    32761 cccctatcag agggaacttg caagtacact gaaagtggac gaagcaagat acacagtaaa
    32821 ttaaaattag acctagccct tttacttcac atgatgagaa atagtaatta tgaaagaagt
    32881 attgaatttg tggataacag aatatctaaa tattctgctc aaaagggaaa atgtgctatc
    32941 acgggtaaat ttctcgaata cgaagaaatc cattgccacc atatcgtacc agtaaaacaa
    33001 cagggtacag acaaatacag caacctcatt attattcaca aaaatgttca cgcattagtc
    33061 catgctatcg aagaaaaaat catacacaaa tacctgaatg tattaaatct aaccaatgaa
    33121 caaatagaaa aactaaatga attacgtgtc aaagctggta atagtgtttt aacagtttaa
    33181 cttgaaaaat atatcatact taacattctt ttgatggaac gccgtatgaa gtgaaagttt
    33241 catgtacggt gtgaagcgg
//
LOCUS       DACEJR010000027        32746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEJR010000027 DACEJR010000000
VERSION     DACEJR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.027"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW651_001294"
     CDS             131..505
                     /locus_tag="IW651_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0814032.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW651_001295"
     CDS             complement(619..1248)
                     /locus_tag="IW651_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0814033.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW651_001296"
     tRNA            complement(1382..1454)
                     /locus_tag="IW651_001296"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IW651_001297"
     tRNA            1668..1741
                     /locus_tag="IW651_001297"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IW651_001298"
     CDS             1696..1845
                     /locus_tag="IW651_001298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814034.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IW651_001299"
     CDS             2338..3666
                     /locus_tag="IW651_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0814035.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IW651_001300"
     CDS             3868..4701
                     /locus_tag="IW651_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0814036.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IW651_001301"
     CDS             4717..5454
                     /locus_tag="IW651_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0814037.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IW651_001302"
     CDS             complement(5609..6577)
                     /locus_tag="IW651_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0814038.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IW651_001303"
     CDS             6809..7642
                     /locus_tag="IW651_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0814039.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IW651_001304"
     CDS             7676..8515
                     /locus_tag="IW651_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0814040.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IW651_001305"
     CDS             8541..9101
                     /locus_tag="IW651_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0814041.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IW651_001306"
     CDS             9300..11021
                     /locus_tag="IW651_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0814042.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IW651_001307"
     CDS             11186..12328
                     /locus_tag="IW651_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0814043.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IW651_001308"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IW651_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0814044.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IW651_001309"
     CDS             14128..14775
                     /locus_tag="IW651_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0814045.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IW651_001310"
     CDS             15168..17813
                     /locus_tag="IW651_001310"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0814046.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IW651_001311"
     CDS             18123..19436
                     /locus_tag="IW651_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0814047.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IW651_001312"
     CDS             19426..20079
                     /locus_tag="IW651_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0814048.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20805
                     /gene="radC"
                     /locus_tag="IW651_001313"
     CDS             20134..20805
                     /gene="radC"
                     /locus_tag="IW651_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0814049.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(20700..22052)
                     /locus_tag="IW651_001314"
     CDS             complement(20700..22052)
                     /locus_tag="IW651_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0814050.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(22118..23407)
                     /locus_tag="IW651_001315"
     CDS             complement(22118..23407)
                     /locus_tag="IW651_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0814051.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(23494..24198)
                     /locus_tag="IW651_001316"
     CDS             complement(23494..24198)
                     /locus_tag="IW651_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0814052.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            24398..24664
                     /locus_tag="IW651_001317"
     CDS             24398..24664
                     /locus_tag="IW651_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0814053.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(24832..25062)
                     /locus_tag="IW651_001318"
     CDS             complement(24832..25062)
                     /locus_tag="IW651_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0814054.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            25134..25289
                     /locus_tag="IW651_001319"
     CDS             25134..25289
                     /locus_tag="IW651_001319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814055.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(25598..25822)
                     /locus_tag="IW651_001320"
     CDS             complement(25598..25822)
                     /locus_tag="IW651_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0814056.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            26089..26424
                     /locus_tag="IW651_001321"
     CDS             26089..26424
                     /locus_tag="IW651_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814057.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            26657..27598
                     /locus_tag="IW651_001322"
     CDS             26657..27598
                     /locus_tag="IW651_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0814058.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            27600..28490
                     /locus_tag="IW651_001323"
     CDS             27600..28490
                     /locus_tag="IW651_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0814059.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            28533..29333
                     /locus_tag="IW651_001324"
     CDS             28533..29333
                     /locus_tag="IW651_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0814060.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            29348..30199
                     /locus_tag="IW651_001325"
     CDS             29348..30199
                     /locus_tag="IW651_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0814061.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            30196..30555
                     /locus_tag="IW651_001326"
     CDS             30196..30555
                     /locus_tag="IW651_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0814062.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            30570..30731
                     /locus_tag="IW651_001327"
     CDS             30570..30731
                     /locus_tag="IW651_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0814063.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            30728..31054
                     /locus_tag="IW651_001328"
     CDS             30728..31054
                     /locus_tag="IW651_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814064.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            31051..31866
                     /locus_tag="IW651_001329"
     CDS             31051..31866
                     /locus_tag="IW651_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ0814065.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            32005..32316
                     /locus_tag="IW651_001330"
     CDS             32005..32316
                     /locus_tag="IW651_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814066.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            32313..32543
                     /locus_tag="IW651_001331"
     CDS             32313..32543
                     /locus_tag="IW651_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814067.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            32550..>32746
                     /locus_tag="IW651_001332"
     CDS             32550..>32746
                     /locus_tag="IW651_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ0814068.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
BASE COUNT        11100 a         5420 c         6654 g         9572 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcaga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgact gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcgcat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tccggaatat ataagttagt acacataaat
    20701 tatataagga aatttactgt tatattgcca tctggaaata ctattatgtt atcgataatt
    20761 gaacgccata atgcattttt ttcttcttcc gaagattctt tataaatttc atcgaattct
    20821 tttgatagaa taaattcgac attttttata tctattttag ctggctctac ttcaatttca
    20881 tttaattctt tcattaaagc ttctcgatct tttttgtatt cctctaagtc tataacttca
    20941 ttcaagtagg ctttcttcaa tctatctaat ttattctcaa tatttttctt actatttttc
    21001 tttttaataa tctttggttg tttttttgaa tagtttattt gtatttgttc aaatttttgt
    21061 ttagtagctt caaaaatata acgttctaaa tttttttcgt aaaaagcgcg cttatttgtg
    21121 caacgtaatt tattatttct tcgttttaca catctataac aagtcctttc gtatatagat
    21181 tctgtaccgt cttttttctt atatcttgct ttaatagttt gaccagctac tgatccctga
    21241 cagtcagaac attttaataa acctttaaaa atgtaatcac gttttgcatt tgtttttaaa
    21301 tttttaggta aggctttttg aacagcataa aaaagttttt tatcaataat aggaggacag
    21361 taatttttat tatttcttaa ttcaccaatg tattttttgt tcgtcaacat atttttaaca
    21421 ctttgataat ctctaacgat tccatactga gagcctaaat aataaacagt ttttcttaaa
    21481 ctaccactag aaagaaagta gttgaatata tctatcacga taggagcatc atcatttatt
    21541 actaatttct tattttctat agagtagcct aacggagttt tcccgcttac tacttcacct
    21601 tgtgcaattt tatttgccat aacagctttg atgcgttctg aatcaatttg ggcttccaat
    21661 tcagcaaaac tcattacttg tgcaatgaaa gttctgccat acgctgtagt tgtatcataa
    21721 tattgttgag aaacagcatt ccaagaaaca ttgtgttttt ctaaaatttc ttgagtgttt
    21781 aaataatgtc gtagatttct aaaccatcta tctaattttg taaataaaat tagatctatt
    21841 ttattttctt ttacatcttg taatagtttt tgaaattcat cacgatttat tttttgacca
    21901 gaaataccac cgtctacata ttcattaact actttcaaat ccttgtgttg ttcgatatat
    21961 tcatagaggg tttctttttg ttctctaaga ctatctccat gtttggcttg ttgttcagtt
    22021 gatactctca tatataatgc tactcgtttc atgttatctc ctttcgaatg tatgttctta
    22081 tcgttgtaaa agaaaagccc gaaggctaat ctttttacta cacatctgtt aattctaatg
    22141 atcttctgta ttgttcagct tgagctagtt tagtaaaaga tacagttccg ttataatttt
    22201 ctaaaacaac tttttcaatt tcatcaagag atactttaaa gaattctttt cgttgattaa
    22261 ctttattgac ttcgtttttt ctaaaatgcg aatgtagttg attttctaat gttggtgcat
    22321 cttctgaaaa tatcattgca tgaacatcaa attcaaaagg tacagaagcg ctactaagtt
    22381 ccttaattct atccataggt tctaaccgtc tagtcattcc tattttatag acatcttcac
    22441 cgaaagatcc gatattactg atgacataaa catagccagc tctagtatta agttctcttt
    22501 gcaatacatc ttctttgaca gtttctagtt ctcttaattt ctcttccagt tctttaattt
    22561 tatcagcata gagttctttt tcaacatcag cactagcttt ttgcatgtat gccattaact
    22621 tagttatctc tgagttaaac tgtcgttctt ctttatcaat gcgtttcttt tcacgttcta
    22681 tttcacgtct taccttttct tcttcaatca tttgttcttt tattgctttt tgctgttctt
    22741 tttcatcgtt aactttaact atgtatgaat agttgagttc taattcttga agttttaatt
    22801 caagtaattc ttttttgaat tctgcttcat cgtttttaaa aagcttgttt attttatcaa
    22861 aagttcttat aattttttct ctagttgaat ctacgttaga aatagtaagt ttattgagaa
    22921 gtccagttat ttctgattct aatggtataa gcattttctt tttaatatta ttagcttctc
    22981 tattattcaa ataagaagga atattaattg caccgtctaa aataaaatcc ttttctttaa
    23041 tctttaataa agaaagtttg ttttttattt cggatgaaga agtttcatct atattcggaa
    23101 taattactga tttatagata gtgaccagag atgtgtcata tttttgatta atttcatcgt
    23161 ctaaccgctg gctttcagca ttaagaatat caattttgtt ttctagcgtt gacaagtttt
    23221 tattcaattt acttatgttt ttctcttttt cagatataaa cgtttcccta gcatttaatt
    23281 cttgagaata ttcctttacc ttactctcta aggcagaagt tttatctttt aattttttag
    23341 cttcagataa tttgctacta cccataatta ttaaaactat tccaactaaa aacaatagta
    23401 ctcccattca aaacgcctcg cttcttttat aaaataattg tcaaaatcat ataaaccctt
    23461 accgaagttg acggctccgg catgggaatt tttttaaaat acaacttttc cgataattct
    23521 aatctgttct ggcgatacaa ttctatcttc gtataagtcg tttatagaac gtaaaatgat
    23581 tgtttgattt tcgtaatctt tatatatctc tttacatgta actccgtcac cacctaattc
    23641 cacaatagca atttccccat cttctacgtc cggttgttct ttaacgaata cgaattgtcc
    23701 attcctgata acaggttcca ttgaatcgcc tataataggt actgcgaagt cagcagcact
    23761 tggaactctc tcaaattctt cctcttcgac aaccgtatca ccgtaagtaa gttctgttgg
    23821 attggcagcc gaagccgaat tgtgaacagt tgggattttt tcttttttag gaaaatctag
    23881 gacattttct tgtttttgtt cttctaatcg attacttgca aattcatagg tagcaacttt
    23941 attttcgtcg ttaagctgat tatatatagg aaggatatta tatatctgat tagatataga
    24001 taaatcaata ttcatcaaat catctatatc aacattgaat atcttagcta tctcatttaa
    24061 ggtttttaat tttggtgtat attttccttt ttcccattcg cttattgagg atgaactttt
    24121 tcttcctagt ttttcggcta gttcttgttg atcaatatta tacttttctc taagaaactt
    24181 tagattttca gcgaacatag taacacctcc ttgagttaat tgtaacaccg tttcggaaaa
    24241 aatgtaacag gtttcttaga aaatatttta gtgtttttaa agaagtattt acgcaaaata
    24301 gaaatcgatt tcaggaaaac tgaaatatgc acttgacttc ggaaaaaccg aagtatataa
    24361 tttagtcaga aagttaaaga gaggaggcga ataaaatatg gataccgcga agaaagaaaa
    24421 atatacattg agacaattaa gagctttaaa gggttattca aaagaagaac tgtcaaggaa
    24481 aagcaaagtg acatcaagaa caatatttat ttacgaaaac gatgtagata aaatgaggaa
    24541 cggaaagtac gccactctag aaaaaattgc taaggcattg ggtgttagag tttccgatat
    24601 ttttttagac cctgattcgg aaaaaccgaa atcaataaca ggtgaaacaa aacaagtcag
    24661 ctaggaggtg tgaatagtgg aagagaagaa tataaaagta ccgactcttg aagaatatac
    24721 aaaaattgtt gtcgaaacag acgagcagaa cccgaaaaca atcgcagtta ttacggcgaa
    24781 tgatattgaa tcatgcgaag gttttcgagt tcgaatgacc cctagatatg attaatgttc
    24841 tttatcacgt ggaggtatag gatcgtttcc aaaactatct tttgattgta tttgtccatt
    24901 ctttttttgg acaataaact ctgattgctg ttttttagaa atctttttac catactcaac
    24961 agcatctgat ttattatcaa atagttttgt atcttcggaa ttaccttcac cacgtacagc
    25021 ccatttgcca tttctaggag atacccattg atttttaccc ataacaattc acctcgcttt
    25081 caagttaatt ctattaagag gtgaaaagaa aacaaactga tggaggtgag attgtgagta
    25141 acaagccaaa aatcactttg aatggcacac ctaatcttca aagattagca gatgaaatgg
    25201 ctagaatacg ttctagacat tggggagtta ctgtggtggc aaccataatt gaacaaaaag
    25261 aaaagcccac acagccggca agcaagtagt gggcaccata gaacaataat attatatcac
    25321 acactcgcca cttccgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac
    25381 gtttcttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg
    25441 atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa
    25501 ttattgaaac agaagaaggg gaaaaagtag cagaagttac attgaccgat gcaactcctg
    25561 ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat
    25621 cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag
    25681 atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat
    25741 gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta
    25801 caacatgttg attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa
    25861 tagaaatgga gaaattcaat aaacgaaagg agggatcaaa tgacattaca agagcatcaa
    25921 cacttgatgc gaaaactaaa tcaggaatac cacgaggaaa tgaaacctag attgctaggg
    25981 gaacaatggc gtgaacgcca gaagaagtgg ttagaaataa aaaagtgact cagccgtcca
    26041 aagcaatgag tcacaaacca aaaattatac ctaaggagat tttatcacat gaataagaaa
    26101 attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa agcaaggtgt ttctattatt
    26161 tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt atggtgagac aacagaaatt
    26221 ttactttgtc ttgctatgca agaagaacat ttagatgaaa atctcccatt gtcagcacat
    26281 attatgcgta gaattgctgt ggatgcttac gaacaagcga aaaatgaaga agaaaatcaa
    26341 ccttcaaatc atacttttgt tattaataac aaggaagatt tagctgatgt gatgactcgt
    26401 attctcaagg gggaatttca atgatgaatg aaaaaacaat tgatctactc attattttat
    26461 ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt tggcttgtta ttcgcaatta
    26521 ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt taaacatgaa cgagttagca
    26581 aatcttgaca actatttaac tgatcctgat tatgctaaac caccatatga agcgccaatt
    26641 gatgaggagg atgaagatga gtaaatctac cttagaaatg agccatcaag aatggctcga
    26701 agaccgcaaa agaggtattg gtggctcaga tgttgcaacc gtacttggat taaacaaata
    26761 caaatctcct tatcaattgt ggcttgagaa aacgggtcaa attgaattga aagatttaga
    26821 aagcgaacca gcttattggg gtaatgtttt agaagaagtg gttgctaaag agtttcagga
    26881 acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt gaacatccat tacatccatt
    26941 tttaagagca aatattgatc gggaagtagt gggagaaaat gccattctgg aatgtaaaac
    27001 agccaatcaa tttctcggaa aagagtggga aggtgaagag gtaccgctta gctatctctg
    27061 tcaagttcag cattacatga acgttttaaa caaagactat tgttatatcg ctgttttgat
    27121 cggtggacaa aaattcattt ggaagcggat tgaacgagat caagagctga tcgatacaat
    27181 cactgaacaa ttagtagagt tttgggaaac gaacgttctt ggaggtatcg agcctgtaat
    27241 tgacggaagt ggagcgactg ctgacttctt aaaagaaaaa tatgcagatg tagaagaaaa
    27301 tcaaacagct ctaccatcac gttttgatga acttatcgag caaaaaagag aactcaagcg
    27361 gacgaaaaaa gaaattgaat catctattcg tcaagtagac aacgagatca tcagtgagct
    27421 aggtaaacgt gaagccagta tcggtatcac acaaagaaac attatcagct ggaaacttgt
    27481 tcgtacaaga cggatgaaca cgaagaaact agcagagaaa tatccagatg tcgcaaatga
    27541 tgaagagatt tataacgtta ctgaatcaag aaggctaacc gaaaaggaga tcaaataata
    27601 tggcaacaaa tgaatcgtta aaaaatcaat tggcagaaaa gccacagaaa caagttgcac
    27661 caggacagtt agggcttaaa gctctaatga atacaccaac aatgagaaag aaatttgaag
    27721 aagtacttca tgacaatgct aatgctttta tgtcgaatgt tatgactctt gtatctaatg
    27781 acagttatct tgcagatagt gaaccgatgt ctatcatgag tggtgcgtta actgctgcaa
    27841 cattaaatct tgggctagat aagaatttag gttatgcata tttagttcca ttcaatagta
    27901 aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt catgcttggc tataaaggat
    27961 atatccaatt agcccaacga tcaggtaaat acaaagcatt aaatgtgatc gaagtttacg
    28021 aaggagaact aaaaagctgg aaccgactga cagaagagtt tgagtttgat ccaaatggta
    28081 gaacgtctga tgaagtcatt ggatatgttg gctatttcga gttactgaat ggattcaaaa
    28141 aaactgtcta ttggaccaaa caagaaatcg aagctcatcg gattgctaac aataaagatc
    28201 gcgataagac aaagttaagt ggtgtgtggg catctgatta caatgcaatg gcacgaaaaa
    28261 ctgttttgag aaatcttctt tctaaatggg gaatcttgtc catcgaaatg caagaagcca
    28321 ccacatcgga tgagagagtc caaagggttc aagaagacgg cagcattatt gctgaaacag
    28381 aagttgaaga agatattcct gaaagaaaag aagcagaggt tatatctgaa gaaaatgaag
    28441 atgtacaaac tggattattt gatgcatcta atccgccgtt aaacaaataa tgagggagct
    28501 ttctcccttg cttttctaga ggagaaatac gaatgaacac aggatatgtc aaattacatc
    28561 gaaaagtgat gaattcattc gtttggacca atccttatat gtataaatta tggagcttgt
    28621 gtttgatgaa agcgagtcat gagaatcgca agatactttt taatggaaaa gagatacaag
    28681 tgaacagcgg agaattcgtc acagggcgcg atgctatcac atctgagatg aacgaaggcg
    28741 tgaaacgtga acatcaagtg aacagcggtt ctgtatggag atggctaaaa caatttgaaa
    28801 aaaacggaat gttgaacatc aaatcaacta cgaaatacag cgttatatca ataaaaaact
    28861 ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa acgcacaaca agtgaacaac
    28921 aagtgcacac aaacaagaat gaaaagaatt ataagaatga aaagaataat aaaacatcat
    28981 cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc agtttactac attctcgcgg
    29041 aggagttatt caaacagatt tgtcagaatc aggaaatcaa aaagccgaat ctgcaaagtt
    29101 gggcagataa cattcggaaa atgatcgaga tcgacaagcg gaccgaaaac caggtacggg
    29161 gaatgattga atggagtcag cacaatgtgt tttgggcatc gaatatttta tctgctaaaa
    29221 aattgcgaga aaaatacgac acaatggcag cgcaagcaaa tcgtgattat aaaacaaaac
    29281 aaactaaaac gcttgaatac gagaaattta gcacagatga gttgcctatt tgagaggagg
    29341 cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg atacagaaag tgcttgtcca
    29401 acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga tggcgtacga agaaccctga
    29461 tggctcagaa cgttgtaagc caacctgtat gcaatgtggg tataaagcat tgcgagttca
    29521 ggaagactta caaacagaac gaatttacaa cgagagcctg aaagcaagag caatcaattt
    29581 tttcaaaggt ggttctgttg tgcctaatca agcgttgttt gattgcacat tgcagaatta
    29641 tcaaattgtc gatcaagaaa caagacaagc ggttgaagta accaaacgct ttgttaattc
    29701 agtcttgtta ggaaatccaa gtcaccttgt tttaactgga aaacaaggaa caggtaaaag
    29761 tcacctagcg atggcagcgg cttgggaagt cttgaagcga tcaaactacg ataaaaaaat
    29821 cttatttatc gggttacagg aaatgctgga tcaaatcaaa ttttcgtaca acaatcctga
    29881 actcagaaaa acgattgagg gatcgttgat tgcagatatc aaaacagcgg atttagtcat
    29941 cattgatgat atcggttcag aactaggaaa agatgcatca gatagtcgag cgtttggcat
    30001 aaacacgcta aattcgttct tggatgcacg acagaaccta gcaacgatta tcacaacgaa
    30061 cttgcttggt gaagaactga aaaaagctta cggtacgaga acaatatcaa gaatgtttgt
    30121 caactctgat ggatttacga tggtattttc tcaaacagca gacaagcgca taaaaccagt
    30181 gaaaggtagt atcgcatgaa taaataccgt aataaaaaaa ctgttcatcg aggtatcaag
    30241 tttgattcta tcgcggaagc agagtattac gatctagcct tgtggcaagc tgaagcgaat
    30301 ggttggaaag tgaagctcca ggaaaggttt gagctgatgc cgaagtttga actagagggc
    30361 aagaagtatc gcaagatcga gtatattccc gacttcacat tttacaaaag cggcaagctg
    30421 gtcaaagtcg tagatgttaa ggggatgcag acaaaagact ttaagatcaa ggcaaagttg
    30481 ttctgtcatc aatatcaagt gccgttgata ttagccaaaa aatatcggaa tacgttcaag
    30541 gaagagcgtt tttaacgagg tggtccatca tgacaacaga agaagtgatt caaatgcgaa
    30601 ttcgaagcct tcggcgtgag attgacgatc tggaacgaac aaaggcagtg atggtcaatg
    30661 aaacggctag aaaggcaatc gatttgcaca tagagaattt aagaagggaa atccgtagat
    30721 tggaggaatg agcgtggata aggaagcggc ttggcgaaaa ttaatgttgc tgattcaaga
    30781 tgagaactgg caagaagatg aagcagtggt tgctgaagtt cagcgtctag gaaagattgc
    30841 taacggacgt atacgaaaaa agccagacaa aagaaaacag cgcaaaggga aaatcgtcgt
    30901 tgttttacac gaaggcaaaa ttttgatgca aggaacagct agtgagctgt ctgcagaaac
    30961 tggatatacg cgtgggacta ttcgaacgta cgcttggcga aatcacaccg atcgaaaagg
    31021 gcacgaatat aagtatttgg aggaagaaaa atgaacgaaa acaaattaat caaattgggt
    31081 gtagcaggag cagtaatcgt aggtattgga gttatcggag gatttaagtt cttcgaaaaa
    31141 atcgataatg gatatgtggg tgtgcgctat tcaatgaacg gcggtatcaa agatgaagca
    31201 ctgacgcaag gtgtgaaatt tgtagggatt gacaaagtga tccaatatcc aattcgcttg
    31261 caaactatcc aatcaaaaaa tatttcagta tctacaagcg acggcaaaaa gacaacgatt
    31321 gatatcaaat atgactacaa agttgattca actaaagcag caaaaatgta caaggaattt
    31381 gggaatatca cttcggaaga tatcgaaagt ggatggttaa aatctaagct tcaaaaggtc
    31441 gctcgtgaag tttatgcgaa atatagtctg cttgatgtcc tttcaggaga ttcctctaaa
    31501 gttgaagctg aggtattaac gaactttgct aaatcagttg aatctaaagg gtttgaagtc
    31561 gaagacgtaa cacttggtgt tccagatgtc gataaagaaa cacaaaaatc aatcgatgcg
    31621 atcattcgag ctggtcaaga aaatgaaaaa gcgaagctag acgcagaaac tgcaaaaact
    31681 caagctgata gtgaagctta caagaaaaca aaagctgcag aagcagaggc agaatctaat
    31741 cgcaaagtcg ccgaatcagt aacagacaat ttgattcgtt atgaagaagc tcaagctcgc
    31801 aaaaagcatg gatgggtaac agtaaatgga gcagatactg tagttacgga tgaagcaggc
    31861 aaataatatg ggattcttta tggctaaaat tctcttgttc ttaggtttag taggagcagc
    31921 atatctcgtg tatgcgctcc tttcccaaac tgatgacaaa gaagatgaca acaacgatga
    31981 aatgaaattt taggaggaga aataatggac gaactaatca caaaagtaga gcagtgggct
    32041 aaagacaagg gactggatca agcagatcca aaagcacagt ttttgaaagt agctgaggaa
    32101 ttcggggaaa tagcttcggc gatggcaaga agtaatgatg aactatttaa agatagcgta
    32161 ggagacgtta tcgtcacgct gattatcctt tccatgcaaa aagggacaaa cgtacaagag
    32221 tgtttagaaa tggcatacaa cgaaatcaaa ggacgcacag ggaaaatggt agatggtgta
    32281 ttcgtgaagt cgagtgattt ggaggatgtg aaatgaaata cgaaatacca ctaagtgaag
    32341 cgggcattca agcaattatc aatggtcggg aggttaacat agaacttcct gatggtactg
    32401 aattagtcat cagacaaagt tatttgaaag atatggcagc tccagtatta attgatcgtt
    32461 ttaacgtgac tgattctgtg gtagagaacc acttaaaaga atttcgatca agtatagaca
    32521 acactttcag attagggagt tgattgacaa tgaacaccag acaccgcaga gtagcaaaac
    32581 taagaaaaca ggaactaaat gtactaaaga caaagtttga aaaagaatat ggaatttcag
    32641 cagaagaaac atataaagtg gcaagtcagt ttgttgctga tgcaagtgat gctattcgta
    32701 agtttgggat ttcgatatta aatgatgatc gtaaatggga ggaaat
//
LOCUS       DACEJR010000028        32590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEJR010000028 DACEJR010000000
VERSION     DACEJR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.028"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..514
                     /locus_tag="IW651_001333"
     CDS             170..514
                     /locus_tag="IW651_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0814069.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IW651_001334"
                     /pseudo
     CDS             515..934
                     /locus_tag="IW651_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IW651_001335"
     CDS             complement(1026..1376)
                     /locus_tag="IW651_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814070.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IW651_001336"
     CDS             complement(1342..2673)
                     /locus_tag="IW651_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0814071.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IW651_001337"
     CDS             2929..3495
                     /locus_tag="IW651_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0814072.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IW651_001338"
     tRNA            3640..3727
                     /locus_tag="IW651_001338"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            complement(3834..4982)
                     /locus_tag="IW651_001339"
     CDS             complement(3834..4982)
                     /locus_tag="IW651_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0814073.1"
                     /translation="MATFEQYKKKNGEKLWKFQTYLGVDPLTGKQVRTTRRGFKTKKE
                     AQLALTKLQLEYESNGLNKTKELTFQEVYDLWIVNYEQTVKESSFVKTKEQFANHILP
                     AFGALKINKISIDIAQKFANEKVKKFVLYREFINNASRICDYAIKLGYLQDNPFKKIT
                     VPKRKVSVHEKDTLNFFSKEELEIFLKSVEKKKDIRMYSFFRTLAFTGMRVGELLALT
                     WKDIDFDDNYIKINKTLARGKNRRLYVEQPKTKNSKRDIPVDDETMNTLKKWRLEQRK
                     WLLTLGINTLSKNQLVFSNQKNEYLQLSKPRKWLEVIIKQNNLKRITIHGLRHTHASL
                     LLEAGANIKDVQERLGHSSIQITMDLYIHITDKRKEKTAAQFAKYIGI"
     gene            complement(5080..5520)
                     /locus_tag="IW651_001340"
     CDS             complement(5080..5520)
                     /locus_tag="IW651_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814074.1"
                     /translation="MKFIKFGIIGLLAVTVMGGCGLSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVNTPLESDQ"
     gene            complement(5606..5806)
                     /locus_tag="IW651_001341"
     CDS             complement(5606..5806)
                     /locus_tag="IW651_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814075.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(5827..6663)
                     /locus_tag="IW651_001342"
     CDS             complement(5827..6663)
                     /locus_tag="IW651_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0814076.1"
                     /translation="MARPPLNNFEKQLRKEISENLKKVTRGKTQAQISQMTGIPASTI
                     SGYFSQRSTIKEENVKKIAKAFGVSEIDIDPRFAHNYDFLRLKKDVMDELSSGKKFNE
                     NDFLQLAKKTSALGVSLNQMKTERGNNITAVYHDTHDYNFFDTSVAAGIPSSVEAFDQ
                     DHIEQIAIPDYAMGKYAGYQDVFFTKVNGDSMNNVIPNGSLIAVKKVMSCEELKDGDI
                     VVFSNDNEFSVKRYINDQQNKRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYIV
                     QL"
     gene            6836..7078
                     /locus_tag="IW651_001343"
     CDS             6836..7078
                     /locus_tag="IW651_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0814077.1"
                     /translation="MKKKISLEAARILTGMTQKEAASKFGVNYQTLASWEQDSNKMKQ
                     KYVQMIPNIYNVLPEEIFFGNKNEFTRYWQGKTVFN"
     gene            7117..7866
                     /locus_tag="IW651_001344"
     CDS             7117..7866
                     /locus_tag="IW651_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAQ0814078.1"
                     /translation="MKELIKVTTNEENEQLVSGRELHEFLEVKERYNDWFPRMREYGF
                     SENVDFISFTEKSVKGGRPQKNHFLKLDMAKEISMLQRTEKGKQARQYFIEVEKEYKQ
                     QLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMDNITEIVALNTTDWRRDCRNFV
                     NKMAQTQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVSKSKRDKTNK
                     LDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            7890..8150
                     /locus_tag="IW651_001345"
     CDS             7890..8150
                     /locus_tag="IW651_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0814079.1"
                     /translation="MEVILTPENEAALRSYIHEIITDEIAKARRDASVDKRVLKQVEI
                     AKYFGVSTATIRKWEDKGLPFGRIGDQKFYDKEKCRAWVLAQ"
     gene            8199..8396
                     /locus_tag="IW651_001346"
     CDS             8199..8396
                     /locus_tag="IW651_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814080.1"
                     /translation="MDKLNTTIVFSAPIIIYLLSVWGSKQALIGVIVYLVWMFAGLDE
                     AEYQTKKRHERRQAESCPEKY"
     gene            8418..8747
                     /locus_tag="IW651_001347"
     CDS             8418..8747
                     /locus_tag="IW651_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814081.1"
                     /translation="MNEKIQNLIKELAAECANDDLGMSVSVVDEKGEVVLGQAGNDSL
                     VAWSVYRQYEKTKRDLQNNNCNCETHGTLKQLFGIEYEDPEFEEWYQDFLRFAEKMDR
                     KKGGIKF"
     gene            8747..8971
                     /locus_tag="IW651_001348"
     CDS             8747..8971
                     /locus_tag="IW651_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814082.1"
                     /translation="MISVKGLGDEIFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQCEDGSVKLFWDF"
     gene            8981..9175
                     /locus_tag="IW651_001349"
     CDS             8981..9175
                     /locus_tag="IW651_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814083.1"
                     /translation="MKDFDSLGARQQPQEEARAVGVDWQGNSLYPGDSCYLTEEGYVP
                     VDDILEYAQQHYPKIELGGI"
     gene            9178..10200
                     /locus_tag="IW651_001350"
     CDS             9178..10200
                     /locus_tag="IW651_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0814084.1"
                     /translation="MANDLTQTIQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSS
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDHSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            10163..10978
                     /locus_tag="IW651_001351"
     CDS             10163..10978
                     /locus_tag="IW651_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814085.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSRMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEVFMNIYQGEKEMIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLFQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKDGEEKPNHCGLCEYCRGNLPITGFTNMDDL"
     gene            10994..11776
                     /locus_tag="IW651_001352"
     CDS             10994..11776
                     /locus_tag="IW651_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814086.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNEKNEKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDF
                     WSGVILSPTSLRKHFDKMAIQKNKRTQQNISNDELPETGEDW"
     gene            11776..12624
                     /locus_tag="IW651_001353"
     CDS             11776..12624
                     /locus_tag="IW651_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0814087.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            12621..12863
                     /locus_tag="IW651_001354"
     CDS             12621..12863
                     /locus_tag="IW651_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814088.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            13006..13461
                     /locus_tag="IW651_001355"
     CDS             13006..13461
                     /locus_tag="IW651_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0814089.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            13463..14038
                     /locus_tag="IW651_001356"
     CDS             13463..14038
                     /locus_tag="IW651_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814090.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNVEYVVNADGTIDMLKEEQTSLDVGDGVEEVE
                     NVKILVSKETIDEFIKTATTLQLPENVTVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            14035..14223
                     /locus_tag="IW651_001357"
     CDS             14035..14223
                     /locus_tag="IW651_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814091.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            14231..14437
                     /locus_tag="IW651_001358"
     CDS             14231..14437
                     /locus_tag="IW651_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814092.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKCPDL
                     FLKDDQIMPEPMILTSKEGGKCES"
     gene            14601..14846
                     /locus_tag="IW651_001359"
     CDS             14601..14846
                     /locus_tag="IW651_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814093.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            14843..15247
                     /locus_tag="IW651_001360"
     CDS             14843..15247
                     /locus_tag="IW651_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814094.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVQWGDTPDWEEEPIRVAVVKINPDIQFDSNVG
                     IFEYGRFIYRDTERFLTVLGNVYENPELLEVE"
     gene            15248..15709
                     /locus_tag="IW651_001361"
     CDS             15248..15709
                     /locus_tag="IW651_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0814095.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            15735..16187
                     /locus_tag="IW651_001362"
     CDS             15735..16187
                     /locus_tag="IW651_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814096.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            16293..16487
                     /locus_tag="IW651_001363"
     CDS             16293..16487
                     /locus_tag="IW651_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814097.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            16518..16817
                     /locus_tag="IW651_001364"
     CDS             16518..16817
                     /locus_tag="IW651_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814098.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGI"
     gene            17059..17475
                     /locus_tag="IW651_001365"
     CDS             17059..17475
                     /locus_tag="IW651_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0814099.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            17791..17997
                     /locus_tag="IW651_001366"
     CDS             17791..17997
                     /locus_tag="IW651_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814100.1"
                     /translation="MLNKASDQVEQLQETLDEIAIVNKAESENSSFFVKANWNIDLPE
                     GDINNPVAEPIKYNPSKAKLDKVH"
     gene            complement(17951..18199)
                     /locus_tag="IW651_001367"
     CDS             complement(17951..18199)
                     /locus_tag="IW651_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814101.1"
                     /translation="MKITAAITKEQGVTFTVVVVKNGVISSSHREQVRSSIPRNFPRP
                     IILAEQSHGKMKYHGRTDIVKFLSNVPYQALPWKDYTL"
     gene            18610..18681
                     /locus_tag="IW651_001368"
     tRNA            18610..18681
                     /locus_tag="IW651_001368"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18643..18645,aa:Lys,seq:ttt)
     gene            18817..19164
                     /locus_tag="IW651_001369"
     CDS             18817..19164
                     /locus_tag="IW651_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814102.1"
                     /translation="MNDFHEAVLSIEVEPSLATAYKKAIEDDNSRHWIKNEIKDADGN
                     IVISDIKPVWNGNYCNVDITDGVRGHSKLTITLLSRTLPNLQEQVDWYKRMGAKVISA
                     NYKGENQNGNEKN"
     gene            19145..19531
                     /locus_tag="IW651_001370"
     CDS             19145..19531
                     /locus_tag="IW651_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814103.1"
                     /translation="MVMRKIRSSITGTEYWDSEKKKTVVVPKGQEPDFEVTEEKGILI
                     DDGSFMTIKGEPITNSNEVLDSDGNTTNDLDGDEVTNDQSVEEADKLDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDILKFIREAE"
     gene            19528..19908
                     /locus_tag="IW651_001371"
     CDS             19528..19908
                     /locus_tag="IW651_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0814104.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            20020..20472
                     /locus_tag="IW651_001372"
     CDS             20020..20472
                     /locus_tag="IW651_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ0814105.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            20469..22196
                     /locus_tag="IW651_001373"
     CDS             20469..22196
                     /locus_tag="IW651_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0814106.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            22260..23447
                     /locus_tag="IW651_001374"
     CDS             22260..23447
                     /locus_tag="IW651_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0814107.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            23413..23988
                     /locus_tag="IW651_001375"
     CDS             23413..23988
                     /locus_tag="IW651_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ0814108.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            24001..25365
                     /locus_tag="IW651_001376"
     CDS             24001..25365
                     /locus_tag="IW651_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0814109.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGD"
     gene            25367..25645
                     /locus_tag="IW651_001377"
     CDS             25367..25645
                     /locus_tag="IW651_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0814110.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            25626..25952
                     /locus_tag="IW651_001378"
     CDS             25626..25952
                     /locus_tag="IW651_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0814111.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            25942..26280
                     /locus_tag="IW651_001379"
     CDS             25942..26280
                     /locus_tag="IW651_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814112.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            26270..26635
                     /locus_tag="IW651_001380"
     CDS             26270..26635
                     /locus_tag="IW651_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0814113.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFCWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            26642..27268
                     /locus_tag="IW651_001381"
     CDS             26642..27268
                     /locus_tag="IW651_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0814114.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            27268..27732
                     /locus_tag="IW651_001382"
     CDS             27268..27732
                     /locus_tag="IW651_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814115.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            27927..30236
                     /locus_tag="IW651_001383"
     CDS             27927..30236
                     /locus_tag="IW651_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0814116.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            30248..30952
                     /locus_tag="IW651_001384"
     CDS             30248..30952
                     /locus_tag="IW651_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0814117.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            30949..>32590
                     /locus_tag="IW651_001385"
     CDS             30949..>32590
                     /locus_tag="IW651_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814118.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPT"
BASE COUNT        11440 a         5170 c         6827 g         9153 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat ataacatttc ataatagagg cgaaaacatt gatatacagg catttgcttg
     3781 caatgttttc gcccctgttt ttttgtttga atacgtcttt gaatacgttc tgcttaaata
     3841 ccgatatatt ttgcgaattg cgctgctgtt ttttcttttc gtttgtctgt aatgtggata
     3901 tataaatcca tagtgatttg aatagacgag tggcctaaac gttcttgtac gtccttaata
     3961 tttgcaccag cttctaaaag taaactagca tgtgtatgtc taagaccatg aatagtaata
     4021 cgtttaagat tattttgttt gataatcact tctaaccatt tacgaggctt agataattgg
     4081 agatattcgt ttttctggtt agaaaatacc agttgatttt tgcttaacgt attaattccc
     4141 aatgttaaca accattttct ttgttctaat cgccatttct tcaaggtgtt catagtttca
     4201 tcatcgactg gtatatctcg cttagaattt ttggttttag gttgctctac ataaaggcgt
     4261 ctattttttc ctctggcgag agttttattt atcttgatat aattatcgtc aaaatcaata
     4321 tctttccatg tgagagctaa gagctcgcct acgcgcatcc ctgtgaaggc tagtgtccga
     4381 aaaaaagaat acatacgaat atcttttttc ttttctactg atttcaaaaa gatttctagt
     4441 tcttctttac taaaaaagtt taaagtatct ttttcatgaa cagagacctt tcttttcgga
     4501 actgtgattt ttttaaaagg attatcttgt agatatccta atttaatagc ataatcacat
     4561 atacgcgaag cattattgat gaattctcta tacaacacaa atttttttac cttttcatta
     4621 gcgaactttt gagctatatc aatcgatatt ttgttgattt taagagcacc aaatgctggt
     4681 aatatatgat tcgcaaactg ttcttttgtt ttaacgaaag aactttcttt tactgtttgt
     4741 tcataattta cgatccataa atcgtaaact tcttgaaaag ttaattcttt agttttattt
     4801 agaccattac tttcgtattc caattgtaat ttggtcagcg ctaattgagc ttcttttttt
     4861 gttttaaaac ctcttcgtgt agttctcact tgtttgccag tcaagggatc tactcccaaa
     4921 taagtttgaa acttccacaa tttttcaccg tttttctttt tgtattgttc gaatgttgcc
     4981 atttttttcg tcctttcgct cgggtaagtg ttcggactaa aatagcttgc atcacctcct
     5041 tagttatacg cttgctttag gctggtaggc gtttatattt tattgatcgc tttctaatgg
     5101 tgtatttacg cctttattag ggtctaaatt actatataga tataggacag tgtaattagt
     5161 cgcatctttt tttgagtaaa tcaaagaaaa gttgtatttt ttgtcttgcc aagtaaaatc
     5221 tccagtacca ttgtagacat ttttgtactc ttcaccagtt tctgcatttt tatcgtcagg
     5281 aaattgagta actttgatag attttaaatc catcttcaaa ttatctatcg aatatatttc
     5341 tttaatgtgt ttttcggcaa tagttgcaaa ttcagcagtt tgatcagagt ctacttcttt
     5401 gatagtactg tttgtctcac tataattttt tgattccttt gtttcagctg ttgataatcc
     5461 acatcctccc ataactgtca ctgctaataa tccaataatt ccaaatttaa taaatttcat
     5521 tttctactcc ttatctctat ggtattattt tattggagaa tcttagaaat aaggtttcga
     5581 gtccgtgttg ctgcacgggc tttttttatt taaataaatc ccaaaaactg aatgtagttt
     5641 tcttgtatac tttattgtaa gcagcctttt ttgggtcttt aatccagccg ctaccttttt
     5701 tcccataacc aggaataact gcttttttta cttttctttt tgcttttcca gtagttctag
     5761 cgctaatgga tttttttata cttggttttc tcattccaat tttcatttta taccctctca
     5821 aattaattat agttggacaa tatatacaac gactttcccg taaatttgaa cgtatttagt
     5881 atcatcataa ttaaccacaa tatcagtgaa agaaatgttg gaagaatcgg gtctgaaaat
     5941 gatacgttta ttttgttgat catttatata tctttttaca gaaaattcgt tatcgttgct
     6001 aaaaacaact atatccccat ccttcaattc ctcacaactc attacctttt tcacagcaat
     6061 caacgaacca tttgggataa cattattcat agaatcccca tttactttag taaaaaacac
     6121 gtcttgataa cctgcatatt ttcccattgc ataatcaggt atagcaattt gttcaatatg
     6181 atcttgatcg aaagcttcga ctgaagaggg gatgccagca gcgactgaag tgtcaaaaaa
     6241 gttataatca tgtgtatcat gataaacagc agttatgtta ttgcccctct ctgttttcat
     6301 ctgatttaaa ctcaccccta aggcgcttgt cttttttgca agttggagaa agtcattttc
     6361 attaaacttc tttccggaac tcaactcgtc cattacatct tttttcagtc ttaaaaaatc
     6421 gtaattatga gcaaatctag gatcaatatc gatctcggag actccaaatg cttttgcaat
     6481 ttttttgaca ttttcttctt ttattgttga tctttgagag aaataaccag aaatagttga
     6541 agcgggaata cctgtcattt gggagatttg agcttgagtc tttccgcgag tcactttttt
     6601 taggttctca gaaatttctt tacgtagttg tttttcgaag ttatttaatg gtggtctagc
     6661 cataactgtc atacctcctg attcattatt atataaaaga ttgtaacgga taaactcgtt
     6721 atttacaact taatagcaat aaaataactt cgttttttaa ataaaaaaac gagtttacta
     6781 attgacataa cgagtaaact cgtttatagt gtttttatga aaaggaggtg tttaaatgaa
     6841 aaagaaaatt tcattagaag ccgctagaat tttaacaggg atgactcaaa aagaagcggc
     6901 ttctaaattt ggcgtaaact accaaacttt agcaagttgg gaacaagatt caaataaaat
     6961 gaaacaaaaa tacgttcaaa tgattccaaa tatttacaat gttttaccag aagaaatttt
     7021 ttttgggaat aaaaacgagt ttactcgtta ttggcaaggc aaaacagtat tcaattaatt
     7081 aaataactaa taaattcgtc gtgaggagtg taatcaatga aagaactaat caaagtaaca
     7141 acaaatgaag agaatgagca gttagtaagt ggtagagagt tacatgaatt tttggaagta
     7201 aaagagcggt ataatgattg gtttccgaga atgagagagt atggattctc tgaaaacgtt
     7261 gattttataa gttttactga aaaatcagta aaaggtggaa gacctcagaa aaaccatttt
     7321 ttaaagttag atatggcaaa agaaatctca atgctccaac gaacagaaaa aggaaaacaa
     7381 gcgcgtcaat attttattga agtagaaaaa gaatacaaac aacaattact tgatacttca
     7441 aagctaagtc cagaacttca aatgttccaa ggaatattta atgcagtagc taagcaagag
     7501 ttagagacaa aacgtttagc aacacaaatg gataatatta ctgaaattgt ggcactgaat
     7561 acaactgatt ggcgtagaga ttgccgaaac tttgtaaata aaatggcaca aacgcaaggt
     7621 ggctatggtg cttatcaaga aattcaaaca gcgatatatg aagaagttga tcgtagagct
     7681 ggttcctctc tcaaaactag attaaccaat ttacgtaacc gtatggctgg cgaaggtgtt
     7741 tcaaaatcca agcgagataa gacaaataag ttagatgtaa tcgaaagtga taaaaggtta
     7801 aaagaaattt atttatctgt agtcaaggat ttcgctatta agtatggagt ttggaaagaa
     7861 aaataaaatt tgataggagg ttcaggatta tggaagtaat cttaacgcct gaaaatgagg
     7921 cagcacttcg tagctacatt catgaaatca taactgatga aattgcaaaa gcaagaagag
     7981 atgcctcagt tgataaacgt gtattaaagc aagtagagat agcgaaatac ttcggagtat
     8041 caactgcaac tattcgtaag tgggaagata aaggacttcc attcgggcgt ataggcgatc
     8101 aaaaatttta cgacaaagaa aaatgtagag catgggttct agcacaataa aatatcgggt
     8161 aagtgttcgg aaataatgac agcaaagaag gggaatttat ggacaaacta aatacaacaa
     8221 tcgtattcag tgcaccaatc attatttatc tgctgagtgt ctggggaagt aaacaagctt
     8281 tgatcggggt aattgtctac ctcgtttgga tgttcgcagg gttagatgaa gctgaatatc
     8341 aaactaaaaa aagacacgaa cgccggcaag cagaatcgtg tcctgaaaaa tattaattac
     8401 aaggagagtt tatcacaatg aatgaaaaaa tccaaaattt aattaaagag ttagcagctg
     8461 aatgtgcgaa tgatgatcta ggaatgtcag ttagtgtagt cgatgaaaaa ggggaagtcg
     8521 ttcttggaca agctggaaac gatagcttgg ttgcgtggag tgtttaccga cagtatgaga
     8581 aaacaaaacg tgatttgcag aataataatt gcaattgtga aactcatggt actttaaaac
     8641 aattgtttgg tattgaatat gaagatccag agtttgagga atggtatcag gactttctac
     8701 ggtttgccga aaaaatggat cgcaagaaag gtggtatcaa attctgatga tctcagttaa
     8761 agggctaggc gatgaaatat tcgaagcaat gatgcacaaa gcacaacaag atgtacaaga
     8821 caaaatctta actgcagcaa agtatggaca aacaagttgc actgttcgtt caaaagggct
     8881 gacaccatca tttctagcag cattagaaag tgaaggtatt tcaaacattc aatgtgaaga
     8941 cggtagtgtc aaattatttt gggattttta ggaggccgtg atgaaagact ttgattcttt
     9001 aggcgcaaga cagcaaccgc aagaagaagc cagagctgtc ggtgttgatt ggcaaggaaa
     9061 ttctttgtat ccaggagatt cttgctattt aacagaagaa ggctatgtac ccgtagacga
     9121 cattctagag tatgcgcaac aacattatcc aaagattgaa ttaggaggaa tttagaaatg
     9181 gcaaatgatt taacacaaac aatacaacgt tcattagacg agcaggtaat cagtaatctt
     9241 gggcggttac aagaacaagg cttagaaatg ccaccaggat atagtccaca aaatgcgtta
     9301 aaaagttctt ttttcgaact cactaacaat acaggaggaa atttgttgca gatggctgca
     9361 aacaatcaag aaatgaaaac ctccatttct aatgcacttt tagacatggt cattcaaggg
     9421 ttatctccag caaaaaaaca atgctatttc attaaatacg gaaacaaggt tcagctaatg
     9481 cgttcatatt ttggaacaat ggcggtcctt gatcgtgtaa ctggaggggc agatattacg
     9541 cctgtagtcg ttcgacaagg tgatgagttt gaagttgcaa tggatggacc gaacatggtt
     9601 gtcaaaaagc atgaaacaaa attcgagaat ttagacaatg aaatcattgc agcctatgta
     9661 gttatcaagt tagcgaatgg taaagagacc acaacagtca tgacgaaaaa gcaaatcgat
     9721 catagttggg cgaagtcaaa aatgaaaggt tctggaccac aaaaggaatt tccagaagaa
     9781 atggctaaac ggacagtaat caatcgtgca gctaaaacat tgatcaacac aagcaacgac
     9841 aacgatttat tagttcaagc tgcaaaagat acgttagaaa atgaatttga caatgatcgg
     9901 aaagatgtaa cgccacaaac agaaaaagtg gctactctcg aacaaaaatt cttttcaaac
     9961 aaaaagatta ctgaaccaat acaaaaagaa ccagatccga ttgtgattcc agatgatatc
    10021 caagatgaag taactcgagt tgctgacgta cctggccatt cagaaataga acaagcacat
    10081 ttaatcgaaa atgaggatac cgatccgatt caagaagaat tattagatat tccggacttt
    10141 ggacgtgagg aaggtgtaga cgatgtctca gaatttgaag acgatgagta ccctttctga
    10201 tgaaaattat tactccaatg aagctgactg gcagtacatg tcgacatcac aatataagtc
    10261 ctttttaaaa tgtgaagccg cagccttagc aaagttgaaa ggcgattggt caccgacgtc
    10321 ggatcccaaa gccttactcg tcggaaacta cgtacattct tattttgaat caaaagaagt
    10381 acatgaagcg ttcaaagaag aaaataaatc cagaatgttt tctagtagga agccgtatgg
    10441 attgttgaaa gatttccaaa ttgctgaaca aatgattgaa cgacttaaac aagaagaagt
    10501 gttcatgaac atctatcaag gtgagaaaga aatgattgtg actggggaac tatttggaac
    10561 gacatggaaa ggcaagattg attgcttgaa tgttgaggat gagtattttg ttgatatcaa
    10621 aacaacaaaa gacatgcatg aacggaaatg gaacgaaaac tatggatcaa gagaaacgtt
    10681 cattgtcaat tttggttacg tgcttcaaat ggcgatttat caggaattac ttttccaaca
    10741 atatggaaaa agttttatgc cgatcatcgc cgcagtgtcc aagcagacac caagcgaagc
    10801 aagactgatc acgattgatc aggacaatat ggattatgag ctggttatgt taaaagaaaa
    10861 aattgaaaga attgtcagag tgaaggacgg cgaagagaag ccgaaccatt gcggtttgtg
    10921 cgagtactgt agaggtaatc ttccaattac tggattcact aacatggacg atttgtagag
    10981 tggtggtgat gaaatgaccg gaggatggat aaagctttat cgccaattac tcgagaaacc
    11041 tatttggcaa gaatctacac cagagcaaaa agttatctta attacccttc tttcaatggc
    11101 gaatcatgaa gaaagagagt gggaatggca aggcgaaccg tacaaagcaa gtccaggtca
    11161 attcgttact tctctcgata gtattagaaa aaaatgtggt aaaggtatct cattgcaaaa
    11221 tgttagaact gctctaaaga ggtttgaaaa atatgaattt ttaacaaacc aaccaacaaa
    11281 caaaaatagg cttataacca ttgtcaactg ggaggtttac caatcaaaag acaacgatct
    11341 aacaaaggaa ctcacaagca accaacaagc aactaacaag caactaacaa ctaacaagaa
    11401 tgaaaagaat gaaaagaatg ataagaataa taatcctcgc aactctcgaa aaacacgaga
    11461 gtatgcagat gacgatccaa ataaaaaatt ggccattctt ttattaaaac tcattcgaaa
    11521 aaatcaaaac atcaaagaac ctgatttgga taaatgggcg aatacgattc gtttaacaat
    11581 tgaatctgac aaacgaactg gtagagaagt tcaagacatg attgtttggg ccacaagtaa
    11641 tgatttctgg tcaggcgtaa tcttatctcc aacgagtttg agaaaacatt tcgataaaat
    11701 ggctatccaa aaaaataaaa gaacgcaaca aaatatttct aatgatgagt taccagaaac
    11761 aggtgaggat tggtaatgaa taaaaaacta agtgcaatgg ctgcacctta cggcggatta
    11821 aaaacagcag atcataattg cccgaaatgt ggtgatccat tgtatatctg gaaaacaaaa
    11881 aataaagatg gtactgatcg atgcggtcct acatgtatca ataagatttg tggttaccga
    11941 gaaatggtaa ctaaaaatca aaaagaagct atccaaaaag cgaatgaagc aatgaaaaag
    12001 gacgctatca atcgaatgct taacagttca atgattacag atgatgccat atggaccttc
    12061 gattttgatg gatacaaagt agttgatcag gaaacagcac aaataaaagc gatggctcaa
    12121 gaatgggcta aaaaaatcgt aagtggcagc acgattcacg cggttattac tggtagaaca
    12181 ggagccggaa aaacccatct aggtgctgca gtgatcaaag aagtaatgat ggcatctaat
    12241 tataaaattg cctgttcatt tataagctat cgagaattat tagagcaatt gaaatttgca
    12301 atgaatgatc cagaagcaag aaaagctgtg acaggttcgt tgatggctga aattaaaaag
    12361 acggattttg tagtgattga tgatttaggt gcggagctag gaagaatgga agaaaacaat
    12421 caggcaacgc cttatgatgt tgatgttctc acatcgctca cagaagctcg tctaaacaaa
    12481 gctacgatat tcacaaccaa tttatcatcg aagcaattaa aacacgcata cggcgagcga
    12541 gtgttctctc gtgtaatgaa tgggacaaaa ggaaacatag ccgtattcaa aacaacgaca
    12601 gacaaaagga ggaatccagt ttgacctttg tagtgaaaaa aatgtgctac ttagatagcc
    12661 gagggcgagg agaagccagt gtcgagtttg ctaaacacca cacgactaaa gaagaggctg
    12721 accttgttgc aagtgtttgt ggcggcgaag tagtcgaagt tattaaaccc aaaagacgat
    12781 tttcaaaacc taagacaaag ccagtgaaaa aagagtgttg ttgtccgaaa agtaaccaag
    12841 catggatgag aggtgcaaaa tgacttgttt aaaatgcaac gatgaaacag ttatttggta
    12901 taagacatcg cttggatggt caacttgtga accttgtcca atatgcaatg aaaatggacg
    12961 acgttcgaaa gaacgactcg aaagactaaa gaaggagtat agcaaatggc aacaagaagc
    13021 aaatacggaa acaaaaagca cgaagtagac ggcatcactt ttgattctaa agcagaagct
    13081 cgttattaca tgaagttaaa acgaaacggt atgagtttta tgcctttatc tgaaacctac
    13141 tgtgccatgc aagaaaatgt tctgctgcaa gaagggtatc tatgcaacga tcgtaagatt
    13201 gcaccgattt attaccgagc tgattttgtg atttatgaga atggccaagt gaaaaaagtg
    13261 attgatgtca aaggttatca agatgcgatc tctatgctga aaatgaaaat gtttgctcat
    13321 cgatacggct ttccagtgac atttgctaag ttcgattcaa aaatcaataa attcattgaa
    13381 atggattgtt ttgaatcagc aagacagcag cggaaaagac aaacggaacg aagaaaaaag
    13441 aaattaatgg agggaaaata aaatgacaaa acaggtgaat ttcagaccag aattaaagaa
    13501 agtaacatca aaatcaaatg ggaacacaga agtattacta gttgttagca acggatcatt
    13561 gagaggtagt actgaaaatt taactgaatt tcttggctca acagtgactg tagtaatcca
    13621 gccagaaact atcgaataca cagtgccagt aaataaacaa acgaaaaaac cgaatgtcga
    13681 atacgttgta aatgctgatg gcacaatcga catgcttaaa gaagagcaga cttcacttga
    13741 tgtaggtgat ggagtagaag aagttgaaaa tgtgaaaatc ctagtatcaa aggaaacgat
    13801 tgatgaattt attaaaacag caacgacatt gcaactacca gaaaacgtaa ctgtaaatat
    13861 tcgagacgtt cttatccgtt tagcagaagg cgatagtatg agtgaaattg ctgcagatca
    13921 tgaattatca gagattgctt taatagatca aatcgaatta gctagacatt actttgctcc
    13981 atatgcagat agctggtcca aacataagga cgacatcatt tttccagagg aacaatgaga
    14041 gcaactgatc cagtaattat ccttgaggaa gctaaattta tttggactca cgaagagata
    14101 gagcaagcac gcttgctctt ttctcaagga gttaagccga gcaaagtagc tgaaataatg
    14161 gatcaaaaga ttcttgatgt cggattgctt ttgttacatc tagcagaaaa aaatttgatt
    14221 tgaggtgaaa atgatgattc aacttgcagg catacaaaca gggaaaattt atttttccgg
    14281 agaaagcaaa agtgaagcta gtcaatggtt gcttaaaaca tatacgaaca ataagaagct
    14341 gagaaaaaaa tgtcctgatc tatttttgaa ggatgatcag attatgccag aaccgatgat
    14401 tttgacaagc aaggagggcg gcaaatgcga atcttagaga ttgtttggga agtaattaaa
    14461 tttatttcca atatgtattt tacgttttgg ttagtgtgct gccttctata tggattcaaa
    14521 tggcaagtag ggaatttcat gagcgttaaa ttgccgggga tattgagacg gagtaaaaga
    14581 atttacaagg aggacagcaa atgataaaaa agctcattca attcagcatg gatttatatg
    14641 atatcgaatc aggagcaaca gtatctgtgg agtctgatca tttaatcata agttttgctg
    14701 acgaacgtca aattatcata tgggtagttg acgatatgct atatccagaa attgtacatg
    14761 attttgaaga atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg
    14821 aaaaatacga ggaggacagc aaatgatacc gaagtttaga gcgtgggata aacgaaagaa
    14881 cgtaatgaga gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa actttattga
    14941 atattggata aatcctaccg aattgaaatc atatcacgtg cgaaacatcg acctcatgca
    15001 atccacagga atgaaagata agaatggtgt ggagatattt gaaggggatg tagtacagtg
    15061 gggagatact cccgattggg aagaggaacc aattagagtt gcagtcgtga agattaaccc
    15121 agatattcaa tttgattcaa acgttggcat atttgaatat ggacgattta tttatcggga
    15181 tacagaaaga tttctcactg ttttaggtaa cgtctacgag aatccagagt tattggaggt
    15241 agagtaattg ccaaaaatat tagatgcatg ttgcggtagt agattgttct ggtttgataa
    15301 gaacaattct gacgtaacat ttatggattg tcgtcagcag tatgaagaat tatcaacagg
    15361 tcatgtgatc aatgttgatc cagatgtggt cgcagacttt cgcgatatgc cttttgaaga
    15421 tgatgtgttc gatatggttg tatttgaccc gccacactta agacatgtcg gcgaaaactc
    15481 atggcttgcc aaaaaatatg gaaaattaga cgaactttgg ccagaagaca tcaggcaagg
    15541 ttttgcagag tgtatgcgag ttttacgacc ttcaggaaca ttaattttta agtggaacga
    15601 ggaacagata ccactttctg atgtgttaga ggctattgga gaacagccat tgtttggtaa
    15661 taaacgaagc aaaacacatt ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca
    15721 aaggaggaaa agtaatgaag attgtattaa ataaatgttt tggtggcttt gggttgagcc
    15781 atgaagcaaa aatggaaatt tttaaaagaa aaaatattga agtattccca tacatcaata
    15841 actttagcta tgactcagat gatgaataca cgagatacac aggccaaaag ctaggttcga
    15901 tggattttat ctactatttc aaaaaagacc cgaaaatcga caaagttaca ggaatttata
    15961 gcgaaattga ccgactgtac ggaattgctg atgattccag tttttcaagt gattcaaaca
    16021 gaggtgataa ggacctcgtt gctgttgtcg aaaaactagg aagtgaggcc agtggaccat
    16081 atgcaagtct taaagtcgtt gatatacccg atggggctga gtgggaaata agtgattatg
    16141 acggcgttga aactgcacat tatggttttc aaacaggtag tatttagtcc actaatgaca
    16201 actaaagagg aggcagaagg atgagtgaag atgaatactt tgaacagtag tctattaacg
    16261 acagagattt ttaactggaa aggggtgtag tcatggacgc atcggaagta gtaaataaaa
    16321 ttaatagtct caaagaggtt ttcggagacg tcgaagtagt gataaacgca caaggctatg
    16381 acaacgaatt ttttaagcag attaccgaca ttagcattca gcaaggaatg gaagacgaag
    16441 acggaaactt tatcgatgaa gtagccattt tagtgacttg cgagtaagta attgacagct
    16501 aaagaggagg aagcggaatg aaactaaaag acggatttta ctccagcggc cacggcatcg
    16561 gcggtttaat gctagatatg ccgacaaaga atcctaaaac acgtaagaaa ccaaaattca
    16621 aagtcggtga catggttcgc tgcgaagcag aaggattcat ctatccattt cgtggatatg
    16681 tagaacacgt ctataatcac tcagcaatca ttcgcattga aaacacgatg gaatgtgata
    16741 agtggttagc gaaaagcaaa gagaatttag cagtggtgag attggtggat atggaggtta
    16801 taaacaatgg aatttaaaat ctttgaagag gacactcgct ataaattaga agaaaaatta
    16861 aatgaatttg caaagaataa tgaaattcag catatatctt taacggcttc taagtccggt
    16921 tatacaactt actatgcagc tgttgtgagc tacgtaagtc aataaaagga actcgataaa
    16981 taaaaaagcc ggatcgctcc gactgttcta ataaattcca caaatttatt atatcacata
    17041 aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt
    17101 aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg
    17161 atagatatta agtcgccgat tattacggat atgccgaagg caccgaagca cggtaataag
    17221 gcagaagacg cgatcattca gatgatggat atagaagcgg agagagacgc aatactagcg
    17281 gctttgatgg ctcttagtct gattagtcgt cagatactct actacagctt ctgtgacgta
    17341 aacaagcact ctaattatga aatagggcaa ttgatacgag gatacggtga aaagaatgta
    17401 gagaagctga aatctatcgc attgattgaa tttgctgaag catacaaaaa aggcgtatta
    17461 gttcagtatc gttgattttg tagggttttt gtagggatag tgtagggttt ttgagtggtt
    17521 taacgtgata ttatgatagt gtcgaaagat tagtgatagg tctaagacaa aataataata
    17581 aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc agcggaaata
    17641 ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc tatcatccgt
    17701 tgctgtctat taatttatgt attggaggga aaacgaatgg ataaagaaat caaagtaact
    17761 gtcaaattag atttgactga actaaaagaa ctgctcaaca aggctagtga ccaagtcgaa
    17821 cagttacaag aaactttaga tgaaattgct attgtgaata aagctgaaag tgaaaattct
    17881 agtttttttg taaaagcaaa ttggaacatt gatttaccag aaggggatat caacaatccg
    17941 gtagcagaac ctataaagta taatccttcc aaggcaaagc ttgataaggt acattagaaa
    18001 gaaattttac gatatctgtt ctaccgtgat acttcatttt cccatgcgac tgttcagcta
    18061 agataatagg tcttgggaaa tttcttggga tgctacttct aacttgttct ctatgagaag
    18121 aagagatcac accgtttttc acaacaacaa ctgtaaaagt tacgccctgt tctttggtaa
    18181 tagcagcagt tattttcata tattcaccac ctataattta tttcagcgga ccactcgctg
    18241 ataaataaaa ttatacgctt agtatttatt ttcacaatat taatttgtca ctgtggcgga
    18301 aagggtagac gctatatctg gtaggtatgc ttgaagacca gagagacaag agctaagcgc
    18361 aaccatgcaa ggttcgattc cttgccagtg actttagtgg atatccaagg taaaaggaac
    18421 ggaagtggga atcaggcatg attcggtagg ataactgcga gggcatatta agttgaccac
    18481 ttaagtaaaa taattaggga gatgcaccta gaaatagagt cggtcgctct atagggtagt
    18541 catcaaatta gactttgcta tggttagacc ctaagccagt aaagttgtgg cggtgaaccc
    18601 ttgccgcttt gacgtgaagt atctgtggtg atacgtctga cttttaatca gaaggtgcag
    18661 gttcgactcc tgtcacgtca attagcaacc gagggtggta tgaactcgtg tggtgcgagc
    18721 cctagggagg aacaggataa ccgcctgtgt gtaggttgct attacatatt agatcactct
    18781 ttgagtggtc tttttatttt gaaaggagtt ttatctatga atgattttca tgaagcggta
    18841 cttagcatcg aggtagaacc aagtctggcc actgcatata aaaaagcgat tgaagatgac
    18901 aacagtaggc attggattaa aaatgaaata aaagatgccg atggaaatat tgtgattagc
    18961 gatatcaagc cagtatggaa tggtaattat tgcaatgttg atattactga tggagtacgc
    19021 ggtcattcga aattaactat cacattgctt tctagaacat tgccaaactt acaagaacag
    19081 gttgattggt ataaacgtat gggtgcaaaa gtaatcagtg caaattacaa aggagagaat
    19141 caaaatggta atgagaaaaa ttagatcatc aattactgga acggagtatt gggattcaga
    19201 aaagaaaaag acggttgtgg ttccgaaagg tcaagaacct gattttgaag taacggaaga
    19261 aaaaggaatc ttaattgatg atggaagttt tatgactatc aaaggtgaac caataactaa
    19321 tagcaatgaa gttcttgata gtgatggcaa cactactaat gacttggatg gagacgaagt
    19381 tactaacgat cagtctgtgg aagaagcaga taaactggac aacatgactg caaaagaatt
    19441 gcgtgcatat gctaagaaac atggtattga tattcctggc gctatccgtg caaaaggcga
    19501 catcctaaaa tttatccgtg aagcagaatg aagtattgtc agtttgacgg atgtacgaac
    19561 aagatagcaa agggaatata ttgtactgaa cacaagagat caagcagatc acgcaagaag
    19621 aagcagcaag caaagtctgt ttatcatcat gagaacaaac cattctatcg aacgcaagca
    19681 tggaaagata tgcgtcaatt tatttatgaa agagaaggtg gtcattgtca gcggtgtggt
    19741 cagttcatat ttggaaagag agcacacgtc catcacattg taccaatcaa agacaacgaa
    19801 ctgcttaagc ttgatccaaa caatctcatg ctattatgtt caaaatgtca tccaattgtt
    19861 gaaaacgaaa cggaagacaa aaaagttttt ccttcgtatt tcaattgaag cccccctatt
    19921 cattttcaaa attttttcgc gtggggagat agggtagcgg ggagtcacgc gcatcgttag
    19981 gtcaaatttt tcaaaaaaca aaggggggtg tatacaaaaa tgacgactaa agcgcaacgt
    20041 aaagcgatta ttgatgaaaa agtaagtgct gaaaaagctc gtatcttaga aataatgaat
    20101 ttgtctgatt tgtacaccat cactcttgat ccattaatcg aatcatactt ggatattttt
    20161 gaaatttacc aacacaaata tttattgtgg aaggaaaaag gatttccgga gacgcaaaaa
    20221 ttcacgaata aatcaggagc tactaatcaa tcaaaacatc cattggcaca acaagtagaa
    20281 acttgggcag ataagaagat gaaagctcta gatttattag gattaacaaa caaagcaaaa
    20341 actggtagac taattgctgg aggatcgacc gcaagaaaag atgaagaaat tacacgccca
    20401 gaagtaaagc cagtagatga actagcagcg cacagaaata aatggcgtaa gaaggcaggt
    20461 gctgaaaaat gattgaacct ggtgtaaatt atgctgattt atttgcaaaa gaagtaagaa
    20521 agaaacctaa gaagtatcct aagaccgttc gtttagcaat agatcgctgg tatcagtgga
    20581 aaaaaagaaa agatatttgg tttgatgtag atcgcgcgaa tgaaatgatg gattgggtag
    20641 aatcatttat tgttcacaca aaaggagaaa tggtgggaaa accgtttctt ttagaaccct
    20701 gggaaaaatt tatttattct tggatttatg gttgggttaa agaaaatgaa aaaggccaaa
    20761 ttgtacgcgt tactcgtgag gcttatgtcc agattccaaa aaagaatggt aaaacattga
    20821 ttgctgtcgg ttcattagga tatgcaatgt acggagaagg ggcgttatca gttgattgct
    20881 atgcttgcgc atctgatttt gctcaagcgc aatatgctgc caaacctttt gcagttacaa
    20941 tcttaaacaa tccaatccta cttgaaggaa ctaaaatatt tagggggcca aagggaactg
    21001 tttctagtat tacttatgac tatattcatg aagatatggc ttactcaaat aaatttattg
    21061 ttcaaaccaa gaatattgat aatatcgagg gatccaatcc atattttgta ttgaacgatg
    21121 aattgcataa acaggagaaa atggaacagt atgacaattt taagtctgct cagatatctt
    21181 taccacagcc attgatgttt aatatctcaa ctgctgggaa aggttcgtcg tctgttggaa
    21241 tgcgtgtata tcgcgaagca aaagaagtgt tgaaacgtga tgataatgat tcaaactttg
    21301 ttctaatcta tgagccaaat aaaaattatg attggacgga tagaaaagtc tgggaaatgt
    21361 gcaatcctaa ctggggaata tcagtcgatt tatctgcttt agaatcggca tttaaaacag
    21421 cgcaacggtc agctcattcc aaagctgaat ttctaacgaa acacttggat gtatttgtga
    21481 acggcgcgga taatttcttt gaacaggatc aagtagagcc atgtttagtt accacacaag
    21541 aacttggtga tttaagtggc gaaccatgtt atatcggttt agatttatca cgtacacgag
    21601 atttgacctg tgtatcttta aacttcccaa catgggatga agatggtaaa gcggttctta
    21661 aagtaaagca actttatttt attccgaatg aagatttaga gtttcgagaa aaagaagata
    21721 atgttcctta cagtgatttg gctgaacaag gtttcgttga attttgcgac ggaaaaatga
    21781 ttgatcaaga tcagattttg cagtacatag aggactgcat gaatttatat gatattcagc
    21841 aagtgaatta tgatccagcg atgagtgaca aacttgttga gaaattagaa aacttaggat
    21901 tggaatgcgt tgaagttgct caatatccta aagtattgaa tgctcctttt gacgatgtcg
    21961 aacggttgtt ttatgagaaa cgaattcaat ttgataatcc attattcctt tattgcactt
    22021 tgaacgttgt agcaattact aacatcaatg gacaaaaagc gccaagtaaa cgtcaatcaa
    22081 agaaaaagat tgatgggttc gtggcgtttt tgtgtggtca taaggaaacg atgaatcaaa
    22141 tgacagatat tgattcagat gagttagatg attacctaag ttccatctat agataaatag
    22201 aaaggcggtg aaaaaaattg aaattacgtg atcggttatc gaatgcagtc tattctttta
    22261 tggaaaaacg tggatatatc gaggatattt ttggacacta cacacgttat ggtcaaagat
    22321 atgtgacgga ttcatctatc atggaatctt ctgatgttta tgaattagtt caagacatat
    22381 ctaatcaggt ggcattggcc acaccaattg ttattggtcc cgacggcaac gaagtcaaag
    22441 atcatcactt actaaatatt ttgaagagtc ccaatgatta tttgacagga ttcgaattta
    22501 ccaaattgga aacaaatact ttattgatca atggtgagac attcccatta acggataggg
    22561 atcagcttca tttggcgtac ggtgtaacaa ctaaaatcaa tgaacgactt caagaagaat
    22621 ttgaaatgaa tggtcaaaaa atacctggtc aaatgattcg acacattaag aacatcggaa
    22681 ctgattcatt aaaaggtgct ggaataattg atcttgcaag aaacactcta gaaggcgttc
    22741 tgagcgctga aaaagttttg acggacaaat atactaaagg cggtttactt gcgttcatgc
    22801 ttaaactaga cgcccacatc aatccaaata atagcgccca aacaaaaatt gtcaaagcta
    22861 tattggatca actggaagga acgcaaaatg agagtgatca ttctgttaag atgattcctt
    22921 tgggaaaagg atattccatc gagacattaa aaagtcctgt tgatgacgcg gcaattttga
    22981 attatttagg tgtttacaaa aaagacttag gaaaatttct aggaatcaat gttgatacgt
    23041 atcaatcgct gatgaagaca gatattgaaa aagcgatgat gtatctgcac aacaaagcaa
    23101 tcaaaccaat attgaaaaac aagagcgaac attacaccgc tctttttttt atgcctaatt
    23161 ctggctatag agtggaatgg aaaattaata ttttggattt tgttccttac tcaacaaaaa
    23221 caaatattgg ctacaacatc gttcgtaccg ggattacaag tccagataac gtggcagaaa
    23281 tgcttggttt tcctaaacag aatactccag aaacacaagc tatctatatt tcaaatgact
    23341 tatctaggat tggccagaaa aatgcaacag atgattcctt accaacgaat gatcaagact
    23401 tgaaaggagg tgatggaaat gaagaagaag gaaattcgca cgattgacat caccaatctt
    23461 tcaacacgtt ccgatgaaga aactcatacg aggaccatta gcggctacgc tgctgtattc
    23521 aacagtcaaa cacttttatg ggatgactta agcgaagtga ttgttcctgg tgcattctca
    23581 aaggcaatta gtaattcgga tgttcgttgt ttgttcaatc acgattggtc caatgtgcta
    23641 ggacgtacta aaagcggaac ccttcgcctt gaagaagatg aacgtggttt gaaattcgaa
    23701 gttgatttac cggacacaac ggtagcaagg gacttagtta aatctatgga acgaggagac
    23761 atcaaccaat gtagttttgg ttttgtgccg actgaagaaa cgtgggacta caattctgaa
    23821 ccaatgcttc gaacaatcca tgaagtggaa ttatacgagg tttctattgt gcctttgcca
    23881 gcatacgaag atacagaagc tgcattaaga agtcgtgatg aattagaaaa aaacgtcgaa
    23941 gaaagaaaaa aattaatcaa aaaaattaat caagcgctag aagcgtagga ggaaaaatac
    24001 atggataaag aattattgaa aaaaatgaag gcacgtcgtg agcaacgatt gactgaatta
    24061 cgtgaaaaag ttgaatcagg agaattacgc gaagcagatt tagaagccgt gaaagaagaa
    24121 attgacagtg ttattgatga attgaacgga attaaagacg aattaggcgc agattctgga
    24181 actgatgaaa cagacgacaa tacagatgat caatcgaata gtacggattc agacgaaagc
    24241 cgttctggtg aagacaacga tcaagaagaa gattcagaca gtgaagatag ttcagaaaat
    24301 cgttctggaa tgattactca acagcaacga gatggattac ttggatcaat taagaacgga
    24361 ttggaggcac gtgcaaaaat gaccaagaaa caaaaagatc aacaactacg aaaagcattt
    24421 gctaattttg tagctggaaa tatttctgaa gcagaagctc gagctttagg gattgaagct
    24481 ggcaacggtt cagttactgt cccagaagta attgcatctg aagttattac ttatgctcaa
    24541 gaagaaaatt tacttcgtaa atacggaaca gtggtgcgaa catcaggaga tgtcaaatat
    24601 ccaattcttg tgaagaaagc agatgcgaat gtaaacaaga aagagcgttc aactgatatt
    24661 gctgaaacag ctattcagtt tgatgaaatt ttgcttaatc ctgccgaatt cgatgctttg
    24721 gcaacagtaa ctaaaaaatt attaaaaatg tctggtgttc cagttgaaga tattgttgtg
    24781 gaagaattga aaaaagctta tgtgcgtaaa gaaatcaatt atatgttcaa tggtgatgac
    24841 gctggaaatg aaaatcctgg tgcattagcc aaaaaggctg tagcatttaa aaaaccttta
    24901 gatctaactg cttcaggtgc tgggcaaaaa ttatatgatg cattaatcga atttaaaaat
    24961 acaccagtga cagaagtgat gaaaaaggga cgctttatta ttaatcgagc tgctttgact
    25021 gccattgaaa aaatgaaaac agatgatgga tttcctttgt tgcggccatt cacacaagca
    25081 gaaggtggaa ttggttacca attagttggc tatcctgtgg attggacaga tgcagcagat
    25141 aaaaagggtg aaccagacac gccagtttta tattttggcg atttttctgc atttaaaatt
    25201 caagaagtta ttggtgcctt ggaaattcaa aaacttgttg aaaaattctc tggtaaaaat
    25261 caaattggat tccaaattta caacttgcta gatggtcaat tggtttattc tccatttgaa
    25321 ccggcagtat atcgctacga aattacaaaa ccagttggtg attaagatgg aagagcaaac
    25381 taaagaattg tctttagagg aaaaattcaa atcacatatt cattttgaag agggcatgga
    25441 tgattctttg ctctcttttt atttaaatat ggcaaaagat tatgtcaaaa cagcaactgg
    25501 tggccaacaa gaatatctta ttttgatggt tgccggcatt gcctatgaat atagagtttc
    25561 agaggatgaa ctcgacaaag ctatgaatgc catgacgcca tttatcgtgc aaggagcgat
    25621 tcaaaatgcc gaagagacag actaataatc tcagatggaa agccgaattg ctagacatca
    25681 aaacaggaac agacggaaac gatcgtccaa ctacaattta cgaatttaag cgtctaatat
    25741 tctatgaaga actcggtgtg acttctcaag aaaaatattt atcacagcaa gccaagacag
    25801 acgttgtcag acgaattaaa gtcagatggg ataaatccat cacagagaaa ttaagtgcgc
    25861 tcaaaattga ttctgtaacg tataacatta ctcgcattta tacgaatccc gatgcaagag
    25921 aaatggagtt gagtttagct tatgtcgatt agctttgatg aattgaaaac agcgctgaaa
    25981 tcaacaaagt tagcagtgtt cagagacaaa gccagattag ggacgatgta tccatacatt
    26041 gtgtactcaa atgtgagtaa cagcaaaaaa atggcatccg gtaaagtcta taaaaaatta
    26101 ccttattacc aagtctcttt ttttacactt ggaacagaac aggatttagt tgttattgaa
    26161 caagcgctgc aaaatgctgg cattccatat tcagatttta caggtataca aggtgatgag
    26221 aacgacgata ccgtaaccaa ctattacaca tatgtgaggt gtgtggaaaa tgccaagtaa
    26281 taagaatggt ttttctgaaa tatcggatta tttaggaaac ctttcaaaag ttgatccgaa
    26341 aaaattatcg ttggaatctt tagaagaagc agctaacttt tatctaaagc agttgcttcc
    26401 taatattcct aagtcgctct taaaaaagaa acatatgcgc gatcaaataa aagtagtagt
    26461 tgaaaacgat caagtgaaag ttcaatttga agagacggcc ttttgctggc gctttgccga
    26521 aaatggcaca acaaatcaac gtgcccaaca ttttgcaagt ggtacgtatg aacaaaacaa
    26581 agaaaaaatt gaagaaatta tgactagaaa aatacttgat ttatgggaag ggtgatttta
    26641 attgggaaaa caagatactt tttattttga aggattggac gacattttaa ttgcgatgat
    26701 ggcaacgcca gattctgttg gaactgcacc gacttatagt gaagttttac gattgccaat
    26761 tgccacaaaa atcggtgtca aagggaatgg aacagcatta gaaaaatggg catccagcaa
    26821 aatgtttcgt cgtgtgtcgc gagaaacaaa acatgaactt gcgttggatc acgtgggtat
    26881 tccgatagaa gtgatggatg aaatcaaagg attagttgct aaaagcggag taacgtttag
    26941 caaaaatacc gcacgagaat ttccgtactt cgcctttgga tttattggaa atattgaaaa
    27001 tggcggtaaa aaagcagttt ggtatcctaa tacacaacta tccaatgtga ttgacgaaga
    27061 gtatgccact gcagaggaag aaacaaaaat tgacgatgta actgcaaatc tggtttccat
    27121 cggactgaaa tacaataatg tgatgtatgc aagttttgat tcaaatagag acggagcttc
    27181 tctagaatta tttaataaat tcattgctca acctgtatac gatgaagaac agtggaaaac
    27241 attggctaaa gttggaggtg cgggttaatg gctcggttat ctgattatgg aattcacgtt
    27301 gaagacttaa aaaattctgc tgttgtaatt attcaaggcg cagaattccc tatttcattt
    27361 actatgcaaa cgatggaatt tatagcagat gtgtatggtg gagattattc gcaatttgaa
    27421 tctgatatga acgccatgct atctaaaaaa gaaggacaaa tttcttctgc taacttatcg
    27481 cctagtgatc taaaaatcat gcgtgccttg atttatgcca tgctgcgtac tggtggttta
    27541 gaggaagatc cagaaactat tttcaaattt ttgggaatga gtggagaggt gttgtctgct
    27601 tatagtacct gtatggaaat ttttgctagc cagacatttc aggtggaaga cctaaaaaaa
    27661 tccaagaagc cacaagactt tcaaaaagcg caagcaaaaa gaaagaaaaa caaaaagaat
    27721 cggaagagat aggaactcct tggagttttt atatttacgt tgctctcact ctattgaatt
    27781 ggagtgagag tttcttttta aagtctacac ctaacttgtg gctcaaatct tatttacagt
    27841 ggttacaaca aaacaccgat tttgaaccac ctcaatctgt aactatggat aaatcgcctt
    27901 ggtggtagaa aggagcgcta agataaatgg ctggtaaaga atccgatgtc gttcttaatt
    27961 ttaaaacgaa tggcgaagtc agttattcga aaacaatcaa agaaatcaac aaagaaatga
    28021 acttagccgc tgccgagtac aaaaaccaag tgtctgcgat ggacaaggat gcaactcaaa
    28081 cagaaaaatt gcgagcggct aaacaaaaat tagaaaaaca attaggattg gctgaacaac
    28141 gatctcaaat gctgagagag gaatatgaaa aatctgtcaa agaaaccggt gaatattctg
    28201 ctgaatcaga aaaactttat aaacaattac tcaattctga aaccggagaa aataaacttc
    28261 gtacagcatt agaacagacg aacgatgcac ttaaagaaca aggtgacgtt tctgttgata
    28321 cagcaaaaaa actccagaaa atcgaagaaa caggtgagaa agtaaaaggc gttggtgaaa
    28381 aaatgtctgt tggagtaacc gcgcctattg tagcggcagg agcagcagga cttgcagcat
    28441 ttggtgaagt tgacgaggca cttgatacca tcattacaaa aaccggagca acaggtgatc
    28501 aagctgatag actttcacag tctttcaaaa acgttggttc aaatactcat ttacctttac
    28561 aaacggttgg ggaagctatt ggtgaggtaa atacacaatt tggattcatg gataaaaaac
    28621 tggaagattc aaccaattat ctcctacagt acgctgaaat caatgataca gatgtttcgc
    28681 aatcagcaat atctgctcga caagctattg atgcttatgg actagaatat gatgatttga
    28741 attctgtcct tgatgtaaca acgaaaacat cgcagaatac tggtcaatct gtagacgact
    28801 tgatgcaaaa agcaattgat ggcgcaccac aaattaaaca attagggttg agctttggtg
    28861 aaggggtcac tttacttgga caatttgagc aatctggtgt tgattcaagc gcagctttaa
    28921 gtagtttatc taaagcaaca gtggcttatg cgaaagacgg aaaatcttta agtcaaggac
    28981 tagaagaact acaagacaag gtaaaaaatg caggttctga aacagaagcc atcaatgcag
    29041 cagcagaagt attcgggact aaaggcggtc ctagaatggc cgatgcaatt cgtagaggta
    29101 ctttgaacct ggaagattta gcaaaaactg ctggagaaag tgggggagct gtaggagaca
    29161 cattcgatgc tactctcgac ccaattgatc aagccgatca agctatgaac aatgcaaaat
    29221 tagcaatggc tgacgtaggt gaagcagtcc aagtaagcct tttgcctttt tttgaaaaag
    29281 caacttcctt attacaggaa ttctctaaat ggtgggggtc tctagatcaa gatacaaaga
    29341 attggatcat aacaattgct ggtatagcgg cagcagttgg accagtacta atcgttcttg
    29401 gttcgctgat gggatctgta acaaaaatcg ttgggggagt taaaagtttt ataggagttt
    29461 ggcaaggact tgctggttta tttggaatgt ctggtggctg gtttgcatta gcagttatag
    29521 ctattggcgc attagttact ggactaattt gggcatataa taacgttaaa tggtttcatg
    29581 atggagtgaa ttctttcttc aaaggtgttt cagatgtagc tgttgaaata tttaattttt
    29641 taagtggtta ttttggtgga tttttcgatg gtgttatagc aaatttcaat aatttttata
    29701 atgctggaaa acgaattttc agtggttttc tagattttat cactggagta tttacaggga
    29761 attggtcaaa agcatggcaa ggtctagttg atatttttgg aggtattttc gatggtattg
    29821 ttgctgtagg gaaagctcct attaatgcca tgattggatt gatcaatgga tttattggtg
    29881 gattgaataa tataaagata ccaaaatggg ttcctggtgt aggtggaaaa tcattttcta
    29941 tttcaaaact gccttattta gcacaaggcg gtcatctaat caatggacaa gcgattgttg
    30001 gtgaagctgg acctgagtta ctgactgcca agaatggtaa aaccacagtt actccattat
    30061 cagacgaaga aaaacgcaaa gggatcagtg gaagagtttc tggaggtagc attgaacagc
    30121 atatacacat tggcaaagtc gatgcaaaca atccatctga gttagatcgt atgaatcgta
    30181 aatttgctaa agcaaacaaa caggctattc gtgatttggg aggtgttcct atttgagtcg
    30241 acaatttatg aatccagatg aaccaaattt tatttggaaa aatcggaatg cagtaattga
    30301 tatgaattgc atcattgagg cagagcttcc agatattatg ccgaacaagc gatacgaaac
    30361 ttacacgatt cagggccgaa gcggagaatt gacggagacg tttgatgact atgaaccttt
    30421 tgatttggaa atagaaggga tcactattcc acattcgaaa ctgagagaag tcaaacgatg
    30481 gctcactggt aaaagccaat taatcacaca taatgatcca gacaagtatc tagaggctat
    30541 ctgcagcatg gataaagaag tccctttcga aaacgagtgg ggcttctttt atacattcga
    30601 tgttactttt cgttgtcagc cactcaaaag aaaaatcggt gaagcaccta aaatttttca
    30661 ttctagtgtt atggatattt ttgatcatgg tgacgaaatt gcacatccct atctggaaat
    30721 agaatcgaac ggaggagata taacgatcgc tgtcggtgat aaaagcctaa caatacttaa
    30781 tacgttagct gcaacaatcg tggttgatac tcagcttgga aaagcaatac aagaaggttt
    30841 gaatttattt acgcgtggtg attggccagt attgcagcct gaatggaatc aggtaaaaat
    30901 atccggaaaa tttaaagaag tgcggttttg gaataggagt gtgtatctgt gacgcaagaa
    30961 tttatttatg cttataaaaa aatgccagat gatttgagcg tgaacggagc gtcgttagtt
    31021 gattgggaag atttgccaga aatcaatcgt gtgctgaatg gccaatatcg tttttatggt
    31081 aactattcaa gaagcggaca gtatcgctct tatttaaaaa aaggaaattt tataaaagcg
    31141 aaggtaccag atggatcatg gcaatatttt gaaatttata atgtcaaaaa gaatttgacc
    31201 tccgtttctg taacagctcg gcatattggt tttatggcca ataagaactt tattgtcaag
    31261 tcgtttacgg ataacggcaa tgggtctcag atcatgacca atcttaaaaa cagtttggca
    31321 tttgatcaaa aatttaacta tctttcgaat gtagggacaa cgcaccaatt tacagcaaga
    31381 caagtcgcgc cagttgaagc aatcattggt tcaaataacg gtaatcaaaa tttgactggc
    31441 gtgactagcg cggaacttga tatggataac tatgatttga aactggtaaa acagattgga
    31501 tcagataatg gttttcggat tgattttggc atcaatttag aagcaatcga agaagaaatt
    31561 gatgaagaat caattgtcaa tagtttgtat ttggttggcg gtgttcctga taacgattat
    31621 gatgaagaca aagaacctat cgaatatggt tatttagaaa ttgatggagt aaccaacgag
    31681 aatcgacgta ttgccaaacg tgaaaacgga gattgtaaaa cagttgatga attgatcaaa
    31741 tggggcaaaa ccttatttga caatgatcgt atccatgagc caaaagctac tcatacagtc
    31801 agtatggtgg cgttggaaca cacactcgaa tacggtgaca tgtatcaaga gctagcgtct
    31861 ttacactttg gtgatgtcgc tcatgttagg gcgaaagagc tagatatcga gataaaagag
    31921 cgtatggtcg aatacactta tttcccaacg cttgggaaat ataaggatat tgttttaggt
    31981 aatgatttat cactgtacac ttcggcagta aatactcaag cacaagagtt gaaaaagaaa
    32041 atcgacaatc gaacagaaac gttagttcaa aatgtgttga atgcgacggc atggatcact
    32101 ggtaattctg gtgggcatgt cgtttttcgt ccagaaaaag ctccatcaga gattcttatc
    32161 atggatactc caaatgtggc gaatgcaaag cgagtgtggc gttggaattt aaatggctta
    32221 ggttattcag acaacggagt aaatggcccg ttcggtattg ctatcacatc aaaaggagaa
    32281 attgttgctg acttcatcaa agtaggaaca ataaatgcag aagtattcga aacttccttt
    32341 aatgcttatg gtgatgtgtt aaaacttgta aaaggcacgc ttcaaatttg gaatgaaaac
    32401 aaaaaaatca tggagctaac caaaaaaggt atggaattct ggaatagaaa agaatccatt
    32461 ggaacgatcg gaacaacaga ttcagcagga aatccatttc caaatgctgt aacgcctaca
    32521 cctcttgagg aaaattcttt ggtaatacgt acaaatggcg atggaaaata cattttaatt
    32581 tctccaactg
//
LOCUS       DACEJR010000029        32367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEJR010000029 DACEJR010000000
VERSION     DACEJR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.029"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..722)
                     /locus_tag="IW651_001386"
     CDS             complement(24..722)
                     /locus_tag="IW651_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0814119.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IW651_001387"
     CDS             complement(715..2067)
                     /locus_tag="IW651_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0814120.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IW651_001388"
     CDS             complement(2290..3690)
                     /locus_tag="IW651_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0814121.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IW651_001389"
     CDS             3851..4588
                     /locus_tag="IW651_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0814122.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IW651_001390"
     CDS             4713..5192
                     /locus_tag="IW651_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0814123.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IW651_001391"
     CDS             5189..5956
                     /locus_tag="IW651_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814124.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IW651_001392"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IW651_001392"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0814125.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IW651_001393"
     CDS             complement(6859..7461)
                     /locus_tag="IW651_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0814126.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IW651_001394"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IW651_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IW651_001395"
     CDS             8142..8726
                     /locus_tag="IW651_001395"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0814127.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IW651_001396"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IW651_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0814128.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IW651_001397"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IW651_001397"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0814129.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IW651_001398"
     CDS             10627..10806
                     /locus_tag="IW651_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814130.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IW651_001399"
     CDS             10828..11853
                     /locus_tag="IW651_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0814131.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IW651_001400"
     CDS             12028..13272
                     /locus_tag="IW651_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0814132.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IW651_001401"
     CDS             13425..14372
                     /locus_tag="IW651_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0814133.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IW651_001402"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IW651_001402"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0814134.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IW651_001403"
     CDS             complement(15274..15516)
                     /locus_tag="IW651_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814135.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IW651_001404"
     tRNA            15637..15709
                     /locus_tag="IW651_001404"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IW651_001405"
     CDS             complement(15828..16466)
                     /locus_tag="IW651_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0814136.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IW651_001406"
     CDS             complement(16589..17221)
                     /locus_tag="IW651_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0814137.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IW651_001407"
     CDS             17572..17871
                     /locus_tag="IW651_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0814138.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18366..20531
                     /gene="acm"
                     /locus_tag="IW651_001408"
     CDS             18366..20531
                     /gene="acm"
                     /locus_tag="IW651_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0814139.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20803..21288)
                     /locus_tag="IW651_001409"
     CDS             complement(20803..21288)
                     /locus_tag="IW651_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0814140.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21768..22409
                     /locus_tag="IW651_001410"
     CDS             21768..22409
                     /locus_tag="IW651_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0814141.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22428..23063
                     /locus_tag="IW651_001411"
     CDS             22428..23063
                     /locus_tag="IW651_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814142.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23081..23896
                     /locus_tag="IW651_001412"
     CDS             23081..23896
                     /locus_tag="IW651_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0814143.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24215..25651
                     /locus_tag="IW651_001413"
     CDS             24215..25651
                     /locus_tag="IW651_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0814144.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25840..28287
                     /locus_tag="IW651_001414"
     CDS             25840..28287
                     /locus_tag="IW651_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0814145.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28517..29584
                     /gene="rlmN"
                     /locus_tag="IW651_001415"
     CDS             28517..29584
                     /gene="rlmN"
                     /locus_tag="IW651_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0814146.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29777..30880)
                     /locus_tag="IW651_001416"
     CDS             complement(29777..30880)
                     /locus_tag="IW651_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0814147.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31433..31981
                     /gene="aac(6')"
                     /locus_tag="IW651_001417"
     CDS             31433..31981
                     /gene="aac(6')"
                     /locus_tag="IW651_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0814148.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        11105 a         5504 c         6572 g         9186 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat
    18181 ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt
    18241 tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa
    18301 gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag
    18361 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
    18421 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
    18481 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
    18541 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
    18601 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
    18661 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
    18721 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
    18781 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
    18841 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
    18901 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
    18961 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
    19021 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
    19081 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
    19141 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
    19201 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
    19261 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
    19321 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
    19381 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
    19441 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
    19501 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
    19561 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
    19621 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
    19681 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
    19741 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
    19801 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
    19861 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
    19921 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
    19981 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
    20041 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
    20101 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
    20161 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
    20221 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
    20281 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
    20341 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
    20401 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
    20461 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
    20521 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
    20581 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
    20641 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
    20701 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
    20761 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
    20821 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
    20881 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
    20941 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
    21001 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
    21061 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
    21121 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
    21181 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
    21241 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
    21301 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
    21361 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
    21421 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
    21481 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
    21541 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
    21601 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
    21661 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
    21721 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
    21781 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
    21841 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
    21901 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
    21961 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
    22021 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
    22081 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
    22141 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
    22201 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
    22261 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
    22321 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag
    22381 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
    22441 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
    22501 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
    22561 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
    22621 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
    22681 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
    22741 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
    22801 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
    22861 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
    22921 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
    22981 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
    23041 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
    23101 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
    23161 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
    23221 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
    23281 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
    23341 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
    23401 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
    23461 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
    23521 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
    23581 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
    23641 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
    23701 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
    23761 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
    23821 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
    23881 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
    23941 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
    24001 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
    24061 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
    24121 caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
    24181 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
    24241 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
    24301 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
    24361 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
    24421 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
    24481 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
    24541 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
    24601 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
    24661 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
    24721 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
    24781 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
    24841 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
    24901 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
    24961 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
    25021 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
    25081 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
    25141 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
    25201 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
    25261 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
    25321 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
    25381 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
    25441 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
    25501 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
    25561 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
    25621 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
    25681 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
    25741 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
    25801 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
    25861 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
    25921 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
    25981 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
    26041 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
    26101 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
    26161 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
    26221 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
    26281 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
    26341 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
    26401 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
    26461 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
    26521 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
    26581 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
    26641 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
    26701 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
    26761 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
    26821 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
    26881 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
    26941 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
    27001 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
    27061 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
    27121 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
    27181 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
    27241 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
    27301 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
    27361 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
    27421 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
    27481 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
    27541 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
    27601 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
    27661 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
    27721 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
    27781 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
    27841 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
    27901 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
    27961 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
    28021 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
    28081 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
    28141 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
    28201 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
    28261 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
    28321 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
    28381 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
    28441 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
    28501 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
    28561 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
    28621 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
    28681 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
    28741 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
    28801 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
    28861 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
    28921 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
    28981 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
    29041 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
    29101 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
    29161 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
    29221 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
    29281 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
    29341 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
    29401 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
    29461 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
    29521 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
    29581 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
    29641 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
    29701 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
    29761 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
    29821 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
    29881 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
    29941 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
    30001 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
    30061 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
    30121 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
    30181 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
    30241 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
    30301 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
    30361 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
    30421 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
    30481 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
    30541 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
    30601 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    30661 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    30721 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    30781 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    30841 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    30901 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    30961 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    31021 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg
    31081 ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa
    31141 aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa
    31201 agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt
    31261 tcctgatctt tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa
    31321 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
    31381 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
    31441 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
    31501 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
    31561 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
    31621 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
    31681 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
    31741 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
    31801 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
    31861 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
    31921 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
    31981 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
    32041 ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac
    32101 cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa
    32161 aatggctttc tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
    32221 ccgtttattc agataaggga ctgtgacaag acttttgtca cagtttcttt ttgtttattc
    32281 ttttttatct ttgtatggca ataatagaca atataacgaa aattatttaa tggcttttgg
    32341 aagaccatgg aaaaccttgt acaaagc
//
LOCUS       DACEJR010000030        32241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEJR010000030 DACEJR010000000
VERSION     DACEJR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.030"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(88..1218)
                     /locus_tag="IW651_001418"
     CDS             complement(88..1218)
                     /locus_tag="IW651_001418"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0814149.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1867..3069)
                     /locus_tag="IW651_001419"
     CDS             complement(1867..3069)
                     /locus_tag="IW651_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0814150.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3129..3332)
                     /locus_tag="IW651_001420"
     CDS             complement(3129..3332)
                     /locus_tag="IW651_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0814151.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3474..4706)
                     /locus_tag="IW651_001421"
                     /pseudo
     CDS             complement(3474..4706)
                     /locus_tag="IW651_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4869..5681
                     /locus_tag="IW651_001422"
     CDS             4869..5681
                     /locus_tag="IW651_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0814152.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6705..6902)
                     /locus_tag="IW651_001423"
     CDS             complement(6705..6902)
                     /locus_tag="IW651_001423"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814153.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7048..7926)
                     /locus_tag="IW651_001424"
     CDS             complement(7048..7926)
                     /locus_tag="IW651_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0814154.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7965..9083)
                     /locus_tag="IW651_001425"
     CDS             complement(7965..9083)
                     /locus_tag="IW651_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0814155.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9080..11179)
                     /locus_tag="IW651_001426"
     CDS             complement(9080..11179)
                     /locus_tag="IW651_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0814156.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11230..12051)
                     /locus_tag="IW651_001427"
     CDS             complement(11230..12051)
                     /locus_tag="IW651_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0814157.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12048..12857)
                     /locus_tag="IW651_001428"
     CDS             complement(12048..12857)
                     /locus_tag="IW651_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0814158.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12878..13372)
                     /locus_tag="IW651_001429"
     CDS             complement(12878..13372)
                     /locus_tag="IW651_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0814159.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13384..13872)
                     /locus_tag="IW651_001430"
     CDS             complement(13384..13872)
                     /locus_tag="IW651_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0814160.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13949..16645)
                     /locus_tag="IW651_001431"
     CDS             complement(13949..16645)
                     /locus_tag="IW651_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0814161.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17073..18146)
                     /locus_tag="IW651_001432"
     CDS             complement(17073..18146)
                     /locus_tag="IW651_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0814162.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18143..18970)
                     /locus_tag="IW651_001433"
     CDS             complement(18143..18970)
                     /locus_tag="IW651_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814163.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18971..19777)
                     /locus_tag="IW651_001434"
     CDS             complement(18971..19777)
                     /locus_tag="IW651_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814164.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19789..20874)
                     /locus_tag="IW651_001435"
     CDS             complement(19789..20874)
                     /locus_tag="IW651_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814165.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20891..21433)
                     /locus_tag="IW651_001436"
     CDS             complement(20891..21433)
                     /locus_tag="IW651_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0814166.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21691..22680
                     /locus_tag="IW651_001437"
     CDS             21691..22680
                     /locus_tag="IW651_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0814167.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22677..23414
                     /locus_tag="IW651_001438"
     CDS             22677..23414
                     /locus_tag="IW651_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0814168.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23411..23962
                     /gene="coaC"
                     /locus_tag="IW651_001439"
     CDS             23411..23962
                     /gene="coaC"
                     /locus_tag="IW651_001439"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0814169.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23978..24610
                     /locus_tag="IW651_001440"
     CDS             23978..24610
                     /locus_tag="IW651_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0814170.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24695..25342
                     /locus_tag="IW651_001441"
     CDS             24695..25342
                     /locus_tag="IW651_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0814171.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25347..26330
                     /locus_tag="IW651_001442"
     CDS             25347..26330
                     /locus_tag="IW651_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0814172.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26733..28247)
                     /locus_tag="IW651_001443"
     CDS             complement(26733..28247)
                     /locus_tag="IW651_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0814173.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28385..29191
                     /locus_tag="IW651_001444"
     CDS             28385..29191
                     /locus_tag="IW651_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814174.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29366..29935)
                     /locus_tag="IW651_001445"
     CDS             complement(29366..29935)
                     /locus_tag="IW651_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0814175.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30018..32078)
                     /locus_tag="IW651_001446"
     CDS             complement(30018..32078)
                     /locus_tag="IW651_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814176.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
BASE COUNT         9649 a         6755 c         5491 g        10346 t
ORIGIN      
        1 ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga ctttttggga
       61 cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga cttgctgcaa
      121 taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt ctgttgaagc
      181 aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt gtgcttcttt
      241 tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag taactaatcc
      301 taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat ctcctgctcg
      361 gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact cttctgcaat
      421 gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc agatatgtgt
      481 tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct ctgtacaaat
      541 atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt tagtttgatc
      601 agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt tttcataaaa
      661 agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag atagatcagg
      721 ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc gtaaaatcat
      781 cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca ctacttcttt
      841 cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc tatctactac
      901 ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc cagtttctgg
      961 aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa atccacgccg
     1021 aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca ctaatttttt
     1081 tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt cttgaacttt
     1141 ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat ctactgtaaa
     1201 tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg gaataagaac
     1561 aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct
     1621 aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc
     1681 cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct
     1741 ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag
     1801 gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg
     1861 gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt
     1921 atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa
     1981 agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg
     2041 ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc
     2101 cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc
     2161 gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct
     2221 gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg
     2281 agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt
     2341 gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc
     2401 agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt
     2461 caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc
     2521 agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc
     2581 ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt
     2641 ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca
     2701 atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg
     2761 cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca
     2821 ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg
     2881 attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc
     2941 atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat
     3001 acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat
     3061 ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct
     3121 tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc
     3181 acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg
     3241 ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca
     3301 cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt
     3361 ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct
     3421 tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag
     3481 cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga
     3541 taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct
     3601 ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata
     3661 aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga
     3721 ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg
     3781 gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta
     3841 agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt
     3901 tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa
     3961 ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga
     4021 ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta
     4081 ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc
     4141 ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta
     4201 aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt
     4261 tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta
     4321 accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata
     4381 tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct
     4441 tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca
     4501 cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca
     4561 cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt
     4621 tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg
     4681 agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag
     4741 aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa
     4801 tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag
     4861 gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag
     4921 aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag
     4981 tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag
     5041 agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt
     5101 tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag
     5161 acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt
     5221 ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg
     5281 aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc
     5341 aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg
     5401 tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt
     5461 tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg
     5521 atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg
     5581 acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga
     5641 aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc
     5701 acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt
     5761 tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc tacgccttcc
     5821 tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga cctggagttt
     5881 gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca taatcagcgc
     5941 gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg gttagttaat
     6001 attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca acctttaaaa
     6061 atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat tctcaaattc
     6121 tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt tccatttttg
     6181 tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga aaaatttttg
     6241 tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc aaattatttt
     6301 tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc ctaataaaga
     6361 aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt cttatttttc
     6421 actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca atcatttcaa
     6481 ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt gcaagagaag
     6541 cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata ggaataaaat
     6601 atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa tcacaactct
     6661 ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca aaaaaattca
     6721 aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca ttggtaagca
     6781 actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa gtgtacgcca
     6841 ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa acgcagaagc
     6901 atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt tttatttgaa
     6961 ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa atacttttga
     7021 catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg cttttctggc
     7081 caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa taatgtagct
     7141 atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat cctgaacttt
     7201 tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat ctggcgaaaa
     7261 catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct gccaaaacga
     7321 atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg ctggtcctgc
     7381 tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat tgtcctttgg
     7441 atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca cgaaagtccc
     7501 attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa cacctgctaa
     7561 ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat ctgttgtcca
     7621 ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat tttgccaagc
     7681 aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa tatcaattgg
     7741 accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa aaaaatcaat
     7801 gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt ccaaaatcgt
     7861 taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa ttgccccata
     7921 tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg cggcaagcgt
     7981 tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata ccagtaagcg
     8041 acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc gatttgttgt
     8101 aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca acgataaaga
     8161 ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt tcccgtttca
     8221 aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa ggctgttgaa
     8281 tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca cgccccgcca
     8341 aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc atcaatgtag
     8401 aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc aaggtaagta
     8461 cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc ctgtaatttg
     8521 gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc caattcgtcc
     8581 accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg taaattctcg
     8641 atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa gccccctgta
     8701 ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc attacaaaag
     8761 aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata aattcttaag
     8821 attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt catccacata
     8881 tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc agaagtcagc
     8941 ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat cgcagcttta
     9001 cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt tttaaattgg
     9061 gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt tttccctttt
     9121 ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac aaatttgaaa
     9181 tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac ttcaaattct
     9241 tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt ttgaatttca
     9301 atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag ccgcatttgc
     9361 cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac ggccaccacc
     9421 accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt acgaaagctt
     9481 gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg gcgccggatt
     9541 ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa gaactagcag
     9601 aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga taaaaaggca
     9661 tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt ccatccacaa
     9721 tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag tgcttcactg
     9781 aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca aaaggaccat
     9841 cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt ttgtatcgct
     9901 tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata caggccttcc
     9961 aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct gataaccaaa
    10021 cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc tcaataattc
    10081 ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc ccagtatgtt
    10141 caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa tgattgatac
    10201 ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat agaaccgttt
    10261 tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac atatccttat
    10321 tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc tgtccctgtc
    10381 gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga tggacaactc
    10441 tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat gtttccttca
    10501 gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca tttgggtcca
    10561 caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta tttccttttt
    10621 gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc acatctttca
    10681 ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga acaagcatac
    10741 ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta aacgtcgaat
    10801 aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata cgatgatccg
    10861 attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac gaattggttt
    10921 gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa ttttctcgat
    10981 aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc ttttgattac
    11041 ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg tattcgtttg
    11101 aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg ggtgaataaa
    11161 ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc tagtagctgc
    11221 agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga ccattccaaa
    11281 aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca atgttaccaa
    11341 taacggtaca attcctttaa aaatactatc gaggatttct tggacattca ttagttcttc
    11401 gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg tcattgcacc
    11461 taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta acccgttcgc
    11521 atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc catagtatcg
    11581 catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca aaatatttcc
    11641 ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc cccagaacaa
    11701 tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt tgatactttc
    11761 cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa tccccatgac
    11821 aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca gcgccgcagc
    11881 tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat aggcgaaacc
    11941 accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc gccaaaacat
    12001 cttccgaacc aattttttct catctttaga aattccatca gctaatttca ttgaaagaaa
    12061 tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac tgcatgaaca
    12121 gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat cgttccaact
    12181 gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc aaagaaaaac
    12241 cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc aactgccggc
    12301 agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt atccaagacg
    12361 cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc ataaagcgca
    12421 tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc accagatttt
    12481 gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat catattcatt
    12541 aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc cacgtttcct
    12601 cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc cgccgtacta
    12661 gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc gacaatcaac
    12721 cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat cggacgactc
    12781 agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc caaagcgact
    12841 acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc aaaatagaga
    12901 tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga taaagttcaa
    12961 ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt gataaggttt
    13021 tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt aattcgattt
    13081 ccgttgggaa agctttcgca acttttaccg catctttgac attagggact aaaaccatca
    13141 agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca actgttttaa
    13201 taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga atatcgttgg
    13261 tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt gtccaagtca
    13321 tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc atttttcttc
    13381 ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa tggtcatctt
    13441 taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta attccttggt
    13501 tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag ttacagcaat
    13561 ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa ataaatcggt
    13621 aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca actcctgttc
    13681 aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct tccccgtcag
    13741 taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta ttagaatttt
    13801 cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc ctgtgccaac
    13861 ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct aacctaaaaa
    13921 aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct aataattcat
    13981 agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc attgattgaa
    14041 aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca taattcggtt
    14101 cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc gtatgcatat
    14161 agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca tatttagaaa
    14221 tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa cggtccgcaa
    14281 ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat tctgccggct
    14341 tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct aagatgtcat
    14401 aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg atgaccaatt
    14461 ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt tctctgtagt
    14521 ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca tctttcaatt
    14581 tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt tgagaaaagc
    14641 cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca atctgtttaa
    14701 atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc gtcaaattat
    14761 tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt tgatttaaag
    14821 agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc acgaaattcc
    14881 ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac gcttcaaaaa
    14941 cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta ctatctccgt
    15001 gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat aaagtcacga
    15061 caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa tgctccaaga
    15121 actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga tttatcccgg
    15181 ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt tctgcaacag
    15241 gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc tttctaaaca
    15301 tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg tgatgcacca
    15361 aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct tcatttatca
    15421 gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga tccgttttta
    15481 tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact tcggattcca
    15541 gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct tcttgtgttc
    15601 gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca atattcccac
    15661 taaagggatg agataacatt aaagacaagg cttctcttga aacaataata tctttttgta
    15721 aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct cgaatggggc
    15781 gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag gtgtctaata
    15841 aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca gaatgccaat
    15901 ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag agcttttctt
    15961 gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg ttggccgttg
    16021 ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg tatccaaaca
    16081 gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag atagcaaatg
    16141 gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt gtggctaaaa
    16201 aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta ggcggatatt
    16261 tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga ccaactacag
    16321 aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca tctaaaggct
    16381 tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact aatctcgtca
    16441 aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc tcggcagtga
    16501 cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct ttagcgctct
    16561 ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc ggctgcaata
    16621 tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc gaatatttgc
    16681 ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat atgattttta
    16741 tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat taatagatta
    16801 cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt aactttttca
    16861 atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc cacttttctc
    16921 tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt ttcatccgct
    16981 tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc tttagaacgt
    17041 tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt gaattctaag
    17101 aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac ttcaagatgg
    17161 gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt tggcaataac
    17221 gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg tgccgcattt
    17281 tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt cacggttttt
    17341 ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac ataatgtaaa
    17401 tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc cacggaggct
    17461 tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac atttggtgtt
    17521 aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact attcaaagaa
    17581 tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat cagcatcaaa
    17641 ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt tgcatcaaag
    17701 aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga atactgatta
    17761 tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc tttgatcttc
    17821 gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat atattcactt
    17881 ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat cgcttcatta
    17941 gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt cgtgatcaaa
    18001 ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc actcatgcca
    18061 cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat gagaatacct
    18121 aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt actcttctgt
    18181 ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca atgaccaata
    18241 tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg attccttgtc
    18301 ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg acgaaaaatg
    18361 taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct atgattcctg
    18421 gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca ccaaggtctc
    18481 tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc atcaagacga
    18541 cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct aatccgaatc
    18601 ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga gagactaata
    18661 agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg taatagattc
    18721 ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa gccagcatga
    18781 acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca taattttccc
    18841 aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa aaaatcagat
    18901 agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac gaccatttaa
    18961 attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc atcacgatga
    19021 acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt tgtgtaacca
    19081 agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca atcagacgag
    19141 tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt ttcacaccat
    19201 ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg cctaaatctc
    19261 tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt ggcataatca
    19321 taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg aataatatct
    19381 gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc acactgaaaa
    19441 tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa ataatcagca
    19501 tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg taactgatga
    19561 tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc atagaaagcg
    19621 atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca ttcatatcaa
    19681 aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg agccacaaaa
    19741 tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct attcttcgta
    19801 gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga catggatatc
    19861 ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg ttgaatggac
    19921 catccattca ttttgctgtt catcataaca gatgatttca taatggacac cacgaaacag
    19981 ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg tcaaatcctc
    20041 tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga cacattcaaa
    20101 ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca cgatattgct
    20161 ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat ctacgggtgt
    20221 accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca ttgccaacgc
    20281 ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt gcagatcccg
    20341 cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg gttcatctaa
    20401 tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct gtctttgacc
    20461 accagacatt tcactaatct cccgtttttc gtatccagga agctggacca tacgcaaagc
    20521 atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca atccaaaagc
    20581 aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga ctgtatttac
    20641 ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga tgtccccttc
    20701 tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac agccggaagg
    20761 accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt ttaatactgt
    20821 ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc ccacgttttt
    20881 accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt cgttttttga
    20941 gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt cctgcttttg
    21001 ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca aaaataaacg
    21061 tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa gtcagcaaga
    21121 caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca tacccgtttt
    21181 cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga ttcgctgctt
    21241 cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa aagaatgttt
    21301 ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt gacaaatctg
    21361 tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt cttaacttct
    21421 caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt cctgtttact
    21481 accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa aatttttttt
    21541 gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt atttttcacc
    21601 taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta tggtatgata
    21661 aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt tcaggtaata
    21721 aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt ggctgaaaat
    21781 gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt ggcaatgagc
    21841 gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt atccggaact
    21901 gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc tactggtttt
    21961 gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc aggagactgg
    22021 ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg tacagcaggg
    22081 tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc cgataaaaaa
    22141 gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct gttacataaa
    22201 ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac atggctaaaa
    22261 agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac aacaaaagct
    22321 ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca gctaagcagt
    22381 cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc tgggggaatc
    22441 aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat tggaattgat
    22501 tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc ggatttgcag
    22561 attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga aacagcatct
    22621 aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga ccataaatga
    22681 agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt gctattacaa
    22741 atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc aatccggata
    22801 ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac tccaatataa
    22861 cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa ctaacgaaaa
    22921 aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact cctgctttta
    22981 gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa gaagaactgg
    23041 acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc tcttctaaca
    23101 ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat ctacgtgaat
    23161 ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca aaggaatctc
    23221 ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt ttcgcaaatg
    23281 atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa gatggaacag
    23341 tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa attagactgg
    23401 aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg cttacaaaag
    23461 cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga ttatgtctaa
    23521 aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc gcgcagtcca
    23581 tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac tagcaaaaca
    23641 agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc tagcaaacgg
    23701 cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa caccaaaatt
    23761 gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc gtaatctgga
    23821 aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt tagcttgtgg
    23881 agattttgga aaaggtgcct tagctgatat tcagacggtc atcgaacagg tcatgctgac
    23941 tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa aaaactttac
    24001 attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt taggttttat
    24061 ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg cttcgatcgt
    24121 tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca gcatgatccg
    24181 tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc cagtcatcgg
    24241 aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca tcttgattgg
    24301 ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa aagcagattc
    24361 cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt tagtgatgaa
    24421 tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa atttaaatgc
    24481 tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag gaatcgtcgc
    24541 aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc acccttctct
    24601 tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt gttactattg
    24661 aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg taggtgcaaa
    24721 cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg aaatttttgc
    24781 aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt cgtatgtctc
    24841 ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca tcgatgtatt
    24901 gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg atggcgcaat
    24961 caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg tcagtgctgt
    25021 ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata ttactaaaca
    25081 ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc aaccagtcac
    25141 attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg agaaggcatc
    25201 aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg aagaaacgga
    25261 tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata cagcattcca
    25321 agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat cctggttctg
    25381 tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc gaattactga
    25441 tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa tcagtctctt
    25501 tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt gagaataaaa
    25561 gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat ctaggtggtt
    25621 atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat acgctagact
    25681 atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca ctttactggc
    25741 ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt ggtgtgggaa
    25801 cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt actgcaggat
    25861 cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc aactataaaa
    25921 aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc gatgttattt
    25981 tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc gttgatggac
    26041 gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac cttggtgtat
    26101 tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt gatcagtata
    26161 aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa aatggcaaga
    26221 ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg catgaatata
    26281 tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa aatcatgctg
    26341 atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga aattcacgaa
    26401 aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc ttctgcttcc
    26461 gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct
    26521 ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca accaagttaa
    26581 aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc
    26641 acaacctctt cactaaacag tgttcaatca gctgaccctc ggtattagct caaaacgctg
    26701 atttcacaac ctctttttat ttacgttcta tttcattcat tttccgaagc aaatcgagct
    26761 gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg caactgaatt tccaccgatc
    26821 cggttgtttc catgtaagcc acctgtgact tcaccagcag catataaccc cctaattggc
    26881 tggttttctt tatctaatac ttcagtgttc gtattgattt tcacaccgcc catcgtgtaa
    26941 tggattcctg gtgcgatttt gatcgcataa taattcggag acgataagtc atgttccatg
    27001 gctgtggcac ggttgaattg agcatctttt ttctccgcaa catccgtatt ccatccatca
    27061 actgtttttg ctagtgtttc ttttggtaaa tcgatttttt ctgctaattc ttccattgtc
    27121 tttccagttg tgacatatcc tttttggtca taaaattcaa tagctgtggc acgatcacgc
    27181 acgccttggt caaaaatcaa ataagctgat ttttcaggca atgctgtaat tgctgcagat
    27241 actttatccc ttgtatccat ttcattgaca aatcttttcc cctcttgatc gaccaagatt
    27301 gctccttctc cgcgaacaac ctcaccaatc aagaccccct catcctgctg cacagttgga
    27361 tgaatttgta ttttatccat atcaacggtt tgtccaccta atttttcaat catggtgata
    27421 ccatctccag ttgatccttc ttgatttgtc gtaatataat cagctaaatc gggacgatat
    27481 ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg taactacgat tgcttttccg
    27541 gtgacctctt ttggttcttc tccttgaaca tgtacagtca cttcattgac cgtttcccca
    27601 tttttcttaa tgtctgtcac atctgcgttg acaaaaatag ggatttcccg ctcatggacg
    27661 ttattcaata acccctccac taagtatcca ccaatagccg agccatcaga cggacgatgt
    27721 gtcctctttt cactcatccc tccagtgatc gttaagttgt ccagtttgat accgttttga
    27781 tctagccaat cgaccgcatc agcagagtga tcgacaaagt atcgaagcat ttctttatca
    27841 ttcgttcctc caccaccttt tagcgtttct tcatagaagg catcattcga gtctttgatt
    27901 ccttcttctt tttgaaattt tgtttctgaa gcattcatac cgcttgatga tttagatgtg
    27961 tttcctccgg caacaggcat cttttccaaa attactggat tcaacccagc atcttttgct
    28021 tcgatagctg ctgccatccc tgcaccacca gctccaataa tcacgacatc ataagattct
    28081 ttcatttctt ttggatctgt atatacttgt tcagaagcgc ctgatgatac ttcggcagat
    28141 tccttcgtac tagaacttgt ctgcgctgta tttttctgat ctgcgccgca tgcgctcata
    28201 gcaagaagtg caacggctgt taataaacca gcaactaatt ttttcatttg tatccccctt
    28261 ttctccttat actatattaa gtgatttttt tcacaatatc aactctgttt ttctttaagt
    28321 catatagctt tggtaaacac acgcttctta gtataattaa ggatatacat gaaggagtta
    28381 ttttatgaat catttgaaaa ccttttttac gaacagtaca aaaactgcag gtagaatcac
    28441 aaacggaatc tatattttat tacgtatcat cgtcgttatt atgctagtgg atcaggtctg
    28501 gaaccaacac tggggaaata caatgctttt attactgaca ttaattttat ttgaaatccc
    28561 ttattttctg gaaaagatat taaagataca gattccaaat atgctagaaa tcattttgtt
    28621 atcttttatt tttagttcaa cagttttagg agagttaagt gatttttatg gctatttttc
    28681 aatgtgggat acttttctac atgcattcag cggattttta gctgggggtg tcggcttttc
    28741 ccttgtttat ttgctcaata aaaatacaaa aaccatgaac ttgactcctt tattactagc
    28801 agttgtttcc ttctgttttt cgatgacagt tggtgttatg tgggagtttg tggaattttc
    28861 agcagataaa ttactgcaat tcgatatgca aaaagatacg atcatccagc aaatcaatag
    28921 tgtggagtta agtaaagacg gaaacactgt ctatcatgtt ccagatatca ctcggacaat
    28981 cattgaaagt caaaatgcag atggtaaaac agataaaaca atcatccaaa atggttactt
    29041 agacgtggga atcatggata ctatgaagga tttatttgtt aattttattg gagcagttgt
    29101 tttcagtgta tttggttacc tctatagtcg aaacgattac caaaaattta cgttcatcaa
    29161 gaattttatt cctaaaaaaa tcaaacgata aaacatgcta aaaaacaaga gaaggggcag
    29221 tgacaatact tttgtcactg ccccttcttt tttgaatgta tggcatgcca ttagttatcc
    29281 gatagtatta actcaaaaaa gtaagatatg aggaatgact atttcgtttt ttatcccaat
    29341 actgaggact tatttcacca tttcgttatt tatctaattt acttaaaacc tgcataactt
    29401 tttcataatc tggattaggt gtgccgtcta gattttcagc aataaaagta aaacccattt
    29461 ttttataaag agcaatcgcc tgatggctcc atgtctgtgt gtgaagatag attggtccgt
    29521 ccgtatggag ggtaggaaat tcttgcaaga ctctggcagt caatgctttt gcgagccctt
    29581 ttccttggta atctggtttc actgctaacc aatgaaaaac cggatacact tgctctcttg
    29641 atttttttcg ccaagcggta caggttgcta ctttttctcc tttttttgtc tctacaaaaa
    29701 acatccttct ttcaagttct tcaggataag gagcaaagtt ttgttgaaaa tactcaagtg
    29761 cttcatctgt cgtttgaaat tccccaacgg aagtctcaat ctccgcccaa gcagattcat
    29821 cttctttttg ataaaatcga aattcaaaac cttccgctaa aggcgtgatt ggaacatttt
    29881 gttgaagtgg tcttgtcatc cagatttctg cgtaaggtaa cgttttatct aacatttttc
    29941 tatccctcct cgtctccatt atgccaaata acatatgact cagctactta tttgactata
    30001 acagatagag atataaatca gaaccagtca tctgcgtcaa attcatttcg attagcgtat
    30061 acccatgttc ctgccatacc tcgcatgaaa tctcctaaag cagaagaagg aatacctcca
    30121 ccaggtgcgg gtgttcccgg actaggaagt ggtacttgcg gctgcagatg ctcatcggtg
    30181 gaatcaatag ttgacaagcg ttttttcttc gtttccttct ggtcatctga ttccttctga
    30241 tcttccgact gtttagcaac aaattctttt tctgattgtt gttttatttt aggctctaaa
    30301 atttcatttt ccaatccaga aacttgtggg gccaaatgag cttcttgcct tatcgtttta
    30361 ttagattctg agatttttct agatattctt gctaactcta ggcttcttgt ttgttttact
    30421 ttccctgttg gagaaactgc atgagccatt tgttgttttt tttctgacaa gctttcttct
    30481 tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg taatagtgac
    30541 tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat cgtaaaatca
    30601 cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt aaccgataca
    30661 tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa ctcttctttg
    30721 tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg attgtctggt
    30781 atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt catcagttct
    30841 gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc gtttgcttgc
    30901 atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa atctatattt
    30961 ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt tacactactc
    31021 atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg agtgatgaca
    31081 gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc cacatttgat
    31141 gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt agtatctact
    31201 gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt tgttacatat
    31261 tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc ttttcctttg
    31321 atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt ttttaaagca
    31381 attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt atccacgata
    31441 ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc gttcttcagc
    31501 ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat tttctttctt
    31561 tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc accgtccttt
    31621 atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc accttttgta
    31681 ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc cgatgtccat
    31741 aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact gactttatta
    31801 taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt atgagtaata
    31861 gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat ggtttgtgtc
    31921 gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag catcgtgcgc
    31981 atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa acgacaagcg
    32041 tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc ctccctactc
    32101 ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa taaaatcaaa
    32161 agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa gaaaatcgga
    32221 ataataactt atgtactttt t
//
LOCUS       DACEJR010000031        31750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEJR010000031 DACEJR010000000
VERSION     DACEJR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.031"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..731
                     /locus_tag="IW651_001447"
     CDS             363..731
                     /locus_tag="IW651_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0814177.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IW651_001448"
     CDS             911..1852
                     /locus_tag="IW651_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0814178.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IW651_001449"
     CDS             1921..2652
                     /locus_tag="IW651_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0814179.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IW651_001450"
     CDS             2796..4976
                     /locus_tag="IW651_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0814180.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IW651_001451"
     CDS             4996..5478
                     /locus_tag="IW651_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0814181.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IW651_001452"
     CDS             5494..6255
                     /locus_tag="IW651_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0814182.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IW651_001453"
     CDS             6386..7720
                     /locus_tag="IW651_001453"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0814183.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IW651_001454"
     CDS             8027..8512
                     /locus_tag="IW651_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0814184.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IW651_001455"
     CDS             8505..9188
                     /locus_tag="IW651_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0814185.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IW651_001456"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IW651_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0814186.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IW651_001457"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IW651_001457"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0814187.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IW651_001458"
     CDS             12804..13433
                     /locus_tag="IW651_001458"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0814188.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IW651_001459"
     CDS             13485..13817
                     /locus_tag="IW651_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0814189.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IW651_001460"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IW651_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0814190.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IW651_001461"
     CDS             14643..16016
                     /locus_tag="IW651_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0814191.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IW651_001462"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IW651_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0814192.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IW651_001463"
     CDS             17071..17511
                     /locus_tag="IW651_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0814193.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IW651_001464"
     CDS             17655..18356
                     /locus_tag="IW651_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0814194.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IW651_001465"
     CDS             18353..19474
                     /locus_tag="IW651_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0814195.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IW651_001466"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IW651_001466"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0814196.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IW651_001467"
     CDS             20853..21761
                     /locus_tag="IW651_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0814197.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IW651_001468"
     CDS             21796..22068
                     /locus_tag="IW651_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0814198.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IW651_001469"
     CDS             22079..23125
                     /locus_tag="IW651_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0814199.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IW651_001470"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IW651_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0814200.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IW651_001471"
     CDS             complement(26136..26807)
                     /locus_tag="IW651_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814201.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IW651_001472"
     CDS             complement(26800..27294)
                     /locus_tag="IW651_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0814202.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IW651_001473"
     CDS             complement(27314..28534)
                     /locus_tag="IW651_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0814203.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IW651_001474"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IW651_001474"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0814204.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31737)
                     /locus_tag="IW651_001475"
     CDS             complement(30610..31737)
                     /locus_tag="IW651_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0814205.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10568 a         5670 c         6794 g         8718 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcttt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaaccatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaacctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atatcttcgc cgtttgtata attaagcgag acaaataaaa aagccattta
    31741 tgttacactc
//
LOCUS       DACEJR010000032        31662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEJR010000032 DACEJR010000000
VERSION     DACEJR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.032"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..345
                     /locus_tag="IW651_001476"
     CDS             <1..345
                     /locus_tag="IW651_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814206.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
     gene            426..1046
                     /locus_tag="IW651_001477"
     CDS             426..1046
                     /locus_tag="IW651_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814207.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1376..2602)
                     /locus_tag="IW651_001478"
     CDS             complement(1376..2602)
                     /locus_tag="IW651_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0814208.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2674..2832)
                     /locus_tag="IW651_001479"
     CDS             complement(2674..2832)
                     /locus_tag="IW651_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0814209.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3520..4260
                     /locus_tag="IW651_001480"
     CDS             3520..4260
                     /locus_tag="IW651_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814210.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4352..5539)
                     /locus_tag="IW651_001481"
     CDS             complement(4352..5539)
                     /locus_tag="IW651_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0814211.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5861..6703
                     /locus_tag="IW651_001482"
     CDS             5861..6703
                     /locus_tag="IW651_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0814212.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6700..7650
                     /locus_tag="IW651_001483"
     CDS             6700..7650
                     /locus_tag="IW651_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0814213.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7665..7898
                     /locus_tag="IW651_001484"
     CDS             7665..7898
                     /locus_tag="IW651_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0814214.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8229..8813
                     /locus_tag="IW651_001485"
     CDS             8229..8813
                     /locus_tag="IW651_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0814215.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8944..9612
                     /locus_tag="IW651_001486"
     CDS             8944..9612
                     /locus_tag="IW651_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0814216.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9614..11038
                     /locus_tag="IW651_001487"
     CDS             9614..11038
                     /locus_tag="IW651_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0814217.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            11056..11625
                     /locus_tag="IW651_001488"
     CDS             11056..11625
                     /locus_tag="IW651_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0814218.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11735..13489
                     /locus_tag="IW651_001489"
     CDS             11735..13489
                     /locus_tag="IW651_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814219.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13443..15215
                     /locus_tag="IW651_001490"
     CDS             13443..15215
                     /locus_tag="IW651_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814220.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            15738..16007
                     /gene="rpsN"
                     /locus_tag="IW651_001491"
     CDS             15738..16007
                     /gene="rpsN"
                     /locus_tag="IW651_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0814221.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            16022..16201
                     /gene="rpmF"
                     /locus_tag="IW651_001492"
     CDS             16022..16201
                     /gene="rpmF"
                     /locus_tag="IW651_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0814222.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            16233..16610
                     /locus_tag="IW651_001493"
     CDS             16233..16610
                     /locus_tag="IW651_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814223.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            16631..16780
                     /gene="rpmG"
                     /locus_tag="IW651_001494"
     CDS             16631..16780
                     /gene="rpmG"
                     /locus_tag="IW651_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0814224.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            16804..17787
                     /locus_tag="IW651_001495"
     CDS             16804..17787
                     /locus_tag="IW651_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0814225.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            17817..17939
                     /locus_tag="IW651_001496"
     CDS             17817..17939
                     /locus_tag="IW651_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0814226.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            17957..19480
                     /locus_tag="IW651_001497"
     CDS             17957..19480
                     /locus_tag="IW651_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0814227.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            19504..19743
                     /locus_tag="IW651_001498"
     CDS             19504..19743
                     /locus_tag="IW651_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814228.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(19936..20397)
                     /locus_tag="IW651_001499"
     CDS             complement(19936..20397)
                     /locus_tag="IW651_001499"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0814229.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(20592..20810)
                     /locus_tag="IW651_001500"
     CDS             complement(20592..20810)
                     /locus_tag="IW651_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0814230.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            20928..22544
                     /locus_tag="IW651_001501"
     CDS             20928..22544
                     /locus_tag="IW651_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0814231.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(22785..24125)
                     /locus_tag="IW651_001502"
                     /pseudo
     CDS             complement(22785..24125)
                     /locus_tag="IW651_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(24401..24556)
                     /locus_tag="IW651_001503"
     CDS             complement(24401..24556)
                     /locus_tag="IW651_001503"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0814232.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(24748..24942)
                     /locus_tag="IW651_001504"
     CDS             complement(24748..24942)
                     /locus_tag="IW651_001504"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0814233.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(24852..25184)
                     /locus_tag="IW651_001505"
     CDS             complement(24852..25184)
                     /locus_tag="IW651_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0814234.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(25698..25889)
                     /locus_tag="IW651_001506"
     CDS             complement(25698..25889)
                     /locus_tag="IW651_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0814235.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(25983..26369)
                     /locus_tag="IW651_001507"
     CDS             complement(25983..26369)
                     /locus_tag="IW651_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0814236.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            27063..28268
                     /locus_tag="IW651_001508"
     CDS             27063..28268
                     /locus_tag="IW651_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0814237.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            28285..28719
                     /locus_tag="IW651_001509"
     CDS             28285..28719
                     /locus_tag="IW651_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814238.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            29336..>31662
                     /gene="esp"
                     /locus_tag="IW651_001510"
     CDS             29336..>31662
                     /gene="esp"
                     /locus_tag="IW651_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0814239.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT        10867 a         4615 c         5823 g        10357 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagctgga gtatgggaag
      301 caaaaaaaga gagataacaa tgatcaatat ctacctaaaa aataacgaat taatgatgca
      361 tatgttaaag ttgttttttt gaattaaaca actcaattta ttcagttata tgttcatcgc
      421 agcaagtgaa cattaatcga gttataaaat taataggagc taaaaatata aataaagttc
      481 gatcagaatt tatttttcca gaagattttt ttaactggtt tttttatcaa ttcagtgaat
      541 gcaagcgact cttaggtgag aattattact taaaaggaat cggcggattt atggggaatg
      601 tggctgatga acaaaatatt ataaaaggaa tatctgaaca aacatcagag cttacagtaa
      661 ctaaagcatt tataagtaat ggtgaaagtt tgaaaaatgt aactgcgcga attagaaaag
      721 aaaatgaagt agaaattgtt tttggaattg accatgaatg taattcagtg atatatgtaa
      781 cacaatcagc aaatttaatt ctttttgaga atggttgcaa ggacgatttt atggtggtct
      841 atttgtacga tatactaatt ccaaaacttc gttccttata tatgtcttct gaaagtattt
      901 ttgtgagtga acaaaaggga tctttctcga aaaaaatagg tattgaagta attgaatcga
      961 ttatgtgtca taatcatatt acgctagaag aattagaaat tttgataggg caaaaaaata
     1021 ataacacgaa aatacaaatt tcttagaaat cattttttat tgagatatct agataaatga
     1081 tttattcttc aatcaaccaa ctcgccaagc tgaggtaatg ttttgaggta atgataacat
     1141 acaacttttt ataccctagt aaaactctat aaacaaagac ttattaattt cgcatgaaat
     1201 acattcctgt aaaatgcaat aaaatagagt tttgtttaac caggtctcaa aaaggctcgt
     1261 aaagcatctc aattctctaa acgttaatat tatttatata tcaatgtttc aaggcacttt
     1321 tcaattgttt gaaaagtgtc tttttcttgt ttaagaacac atttaagaac acatactaat
     1381 cacatttatt caatcctctc tgaaaggctt caaaaacagt tttatctact acatctggtg
     1441 tatttacgta tattctcgtt gtagaaatat ctgagtgtgt taaagcacta gatatttgat
     1501 tcatatttgc tccgccttgt ctagctatag ttgcataggt atgacgaagc ttatgtggag
     1561 ataaatgagc caattcagga tgtcgtcttt taattgaatt gattcgatag tttaggtaat
     1621 cagcgtgaac agggcaattt acttctccgg atggtttagt ataggtaaag agatactgat
     1681 ctggtgtttg attaattttt aatctcaaca attgctcagc ttgcactgat ttccatttgc
     1741 ttagtaatgc cattacaatt tcgggtactt gaattacggt atcctttcga ccttttgtaa
     1801 atttcttttt ttgatattta tccaaagcat gtttaaggcg aaccgtttgt ttctcaaagt
     1861 cgatatattt ccattgcaaa gcatacgtct cactttttct atcaccaaga tatagggtaa
     1921 gcatgaaaag agtatagtca tgaagagtaa gcaagtgatt attcaagtca gtgtttactg
     1981 cttctatcca agcaataagc tcttctgacg ataatgcttg attaccttcc cgtatccttc
     2041 tttcttctaa agcaattttt ttaggtgcag tgatacattg aataattttg gtagttcggt
     2101 taaattcaat ataatttagt atttctgcaa tatcaaacat agaacggaca tatcctttga
     2161 taatttttat atttgcgtat tctttcgatt tttttgttaa ttcatctgat attatctcag
     2221 tatttgaatt tagatagttc attgcatact caccaaacat tggaaggatg tgcaatcgga
     2281 atatatcttt agtattacta attgttacgt aacttggagc tctttttgat ctagtagtct
     2341 gacctagttc atatctagga agccattttg attcatagaa ttccttaaat tttatttttc
     2401 cttttaattc tagtgaattt gcattctcct ctcttaaaac tgtttcaatt ttccttttca
     2461 ttgcattttc ggctttaata gccaattgcc tagttggaaa tactttttga tacttttctg
     2521 atgtaattcc agtgatttct ctgaattttt ttggatacct cagattagtt ttatatttcc
     2581 catttgaaag ttttgtaggc aaaattcctc actcccttaa aaagtaaggc agtatatttc
     2641 gctattctta gtatatgcgg cgagaagaaa ttttcaaatt gttttaaatg tattcataaa
     2701 atctttgata tcctctatgt caaagcgaac tataccgtca attctgatta ctggaaaatt
     2761 atatatacga atccacttat ctaaagtatt attatcaatt cctagaaact cacaggtttg
     2821 ttttttattc aagtagactc tcaattttat aaaatcatct cctaaaaaag attagaattt
     2881 ttgaattgct ttgggtaagc acataggaat ttcacctctc cccagtttac ggcgagcctt
     2941 acggaagtat cattatttgc tgctattgtc atggcatttt gttttcccag aacaaaaaga
     3001 aatctctaag tatttgtcgc taatcaggtc atggcgtact tgagtttcta gctcgaatag
     3061 cagggatcgt tattcaaaaa ttgtatttaa ttgtcaatga tcaaaaatag gttttttcct
     3121 attcactatt aataacgata aacatgtata aaaagacaca ctacgaaaaa agttaaatgg
     3181 agtttactga ttttcatatt tgtatatgga acagacatag tcttgaataa tttttataaa
     3241 tgttttgatg cttatcaact tattcaaccc gctatccact ttaagtataa tttttccata
     3301 cgccaataaa gtagttttag gagcatgttc tcatttgaga acgctccttt tttatatatt
     3361 ttgaaagcta cttactatac gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa
     3421 cagaaaaaaa tctaaattat aaaatttagg gttgactaaa atataatttt tgtataccat
     3481 atgatcgtga taatcattat catttagaag gagaaagtta tgttagaagt gaaaaagctt
     3541 aatgtttgtt ataacgattt tttagcattg aacgagattt ctttaaaaat acaagaaggt
     3601 gctttaacag gtattattgg tccaaatggt gctggaaaat ctacattgct taaagctatg
     3661 ttaaatatta tccctcatca gggagaggtc tccattaata atgaagatat caataaaaaa
     3721 ctggtgaaga ttgcatatgt agagcaaaaa gctgatattg attttacttt tccaataaaa
     3781 gtaaaagaat gtgtatctat gggaacatat gccggaatga aagtttttca aagaattaaa
     3841 aatgcagaat ggcaaagagt ttcaaaggct ttggaaaagg tcgatatggg aaaatattct
     3901 aatcatcaaa taggagaatt atctggagga caatttcaac gtgtactttt agcaagatgt
     3961 cttgctcaaa atgcggattt catattttta gatgaaccat ttgtagggat agacttagtt
     4021 agtgaacgaa taattatgga tacgttaaaa gaattgaaac atcagggaaa aacagtgcta
     4081 attgtgcatc atgatttaag caaggtaaag aagtattttg ataatattat tcttttgaat
     4141 cgaaatctaa ttgctcacgg aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa
     4201 gcctatggag atactatatt tattggagag actaactata tagagtcaag taaaaattag
     4261 ccaaatcaaa aaatatttta taacagataa ttccgaaaaa agggtacaac aaataagctt
     4321 cttaccatta gtttttttga atgttcaata gctaatcaga aacaattata taaggctcgt
     4381 ttttagtcaa aatagcatga atcacgtaac aaagcttacg agaaaccgca cctaaggcag
     4441 ttagatgatg ttttccttcg gaaacttttt tctggtaaaa cgcactaaaa acaggatcac
     4501 attgagaagc tactaaagcc gccgaaaaca aggcttttcg caaatatgga gaccctcgtt
     4561 tactcataac attatgcgtt gcctcatatt gccctgattg tgtcacggaa gcgtctatcc
     4621 cagcgtaagc aacgagtttt ttcgggttag aaaatttttg aatatctccg atctcaccaa
     4681 gaatagtcgc accgtttata ggaccaattc caggaattgt gagaataaca gaatttaaag
     4741 tgtccattat gtgcttgatt tctctttccg tttcctttat ttggctttca atgaatttta
     4801 attgttcaat cattgaacgt agttggaagg tgaacgcttc ttgagcaaat tttactccaa
     4861 aggagttaga agctgcttgc ttcaattggt ccgctttttc tgccttgatt cggttcctgc
     4921 ttaactcagc taagtattgg gccaaagttt ctgaggaaat gttttctaag tcgatcgggg
     4981 cactaaacgc cagcaataac tctttagaag cttttccgaa aacgccctgc ttggtaaaaa
     5041 tcgtcgcgta ttctggaaag acttgatcca atacggctat aatttttctt ttgaagtcgc
     5101 tcgctgtacc tactaaataa gttcggtaac gagaaagttg tttcaaagaa aaaacatttt
     5161 catcggataa gaccgtttcg acaaatgagc catagcgcat taagtcagca atcaaaacag
     5221 aatcaataat atcgtttttt cgtttccgta tttctgtacc tttacgccat ccgtctgttt
     5281 gaaggggatt cacaacgtga atcaaataat ctgattcgtg aaggtatgaa aagatggaca
     5341 gccaatagtg ccccgtggct tccatcccta taacaaagtc actgggtgct ccggaatagt
     5401 cgaccattcg ctcaattaat gactctgctc cttcagtggt gttaggaaat gaaaaacctt
     5461 ttagtaatac ctttccttcc tcgttcatga tagaagcaac gtgagtgcgt ttaccgatat
     5521 caatgcctaa aaagtacatg tatatctgac ctttcaaaaa aagaattttg gattgaaatc
     5581 cactcaactg aaaagcgtca ctaccttgta cgagatacgg agtaaagtaa ggactttcaa
     5641 catctaactc atacgcaatc cacttatcag agagaggagc cagtctctcg agtgcgggtt
     5701 caatggccca aggggggaac ggcttagcct ctcaatccaa taaatatatt atctcaaaag
     5761 aaatgagtta ataccaaatt aaaataacga tggttcatag ggttatccaa tcaaaaccgg
     5821 aaggttaaaa ccctaactct aatatacaag ggggaataat atgcttcaaa attttattaa
     5881 tggtctgtat gactttcact ttttacagaa tgctctaatt acatctgtag ttatagggat
     5941 tgtttctgga acagtaggtt gttttattat tttaagagga atgtctctta tgggtgatgc
     6001 tatttctcat gctgttttgc caggagtagc aatttcttat attttaggta ttaatttttt
     6061 tattggtgcg attgtatttg gtctattagc ttctatcatt attactttta taaaaagcaa
     6121 tagtgtaata aaaggagata cagcaattgg tattactttc agttcttttt tagcattagg
     6181 agttatcctt ataggagttg caaatagctc aacggattta tttcatatct tatttggaaa
     6241 tattttagct gtacaagata ttgataagtg gattacaatt ggcgtttctg ttttagtgtt
     6301 ggttacagtt gttctatttt ttaaagaatt attaatcact tcttttgatc cattaatggc
     6361 aaaagcaatg gggatgaatg tcagttttta tcattatcta ttaatgattc tattaacttt
     6421 agtagcagta acagctatgc aaagtgtggg aactatatta attgttgcaa tgttaattac
     6481 accagctgct acagcatatt tatatgcaaa cagcttaaaa actatgatat ttatttcggc
     6541 gacagtcgga gcgtgctcat cattgctagg tttgttcatt ggttatagtt ttaacatagc
     6601 agcaggatca agtattgttt taacagcggc attcttattt gtaattagtt ttctaatctc
     6661 accaaaacaa aaatttttaa gaaagaagga gagaaagtta tgatgagaaa atggaaagca
     6721 gtactgggaa gtctggggat tttgattgct ctttttatat tcggggcatg ttcaacaaat
     6781 agtaaagaca aagatacagt ggcttcgaac gaaaaattaa aggtagtagt tactaattcg
     6841 attttagcag atattactga aaatatagca aaagataaaa ttgatttaca cagtatcgta
     6901 cctattggga aagatcccca cgaatatgaa cctttgcctg aagatgttca aaaaacttca
     6961 aaagcagatt tgatttttta taacggtgtt aacttggaaa ctggaggaaa tgcttggttt
     7021 acaaaattag taaaaaatgc gaacaaagag gaaaacaaag actattttgc agcaagtgat
     7081 ggcatagatg ttatttactt agagggtcag agtgagaaag ggaaggaaga tccccatgct
     7141 tggttaaatt tagaaaacgg tattatttac gctaaaaata ttgaaaaaca gttagcggaa
     7201 aaagatcctg ataataaaaa attctataaa gaaaatctag ataagtatat tgaaaaattg
     7261 gattctctag acaaagaagc taaatctaaa tttgcttcaa ttccgaatga taaaaaaatg
     7321 attgttacaa gtgaaggatg ctttaaatat ttctcgaaag cgtataatgt gccttctgct
     7381 tacatttggg aaatcaacac tgaagaagaa ggaacaccag atcaaataaa acacttagtt
     7441 gaaaaattac gcacaacaaa agttccctcc ttattcgtag aaagcagtgt ggacgataga
     7501 ccaatgaaaa cagtatcaaa agataccaat attcctatct attcaacgat ttttactgat
     7561 tcaattgcag aaaaaggaca agatggtgat agttactatg cgatgatgaa atggaacctg
     7621 gataaaattg ctgaaggcct ttcgaaataa tttggagggt aggtatggta caaattaaaa
     7681 cgactactat actaatttat ttactagcaa ttcaggaatt atcaaaaaga aaaaagaaat
     7741 taaaaaataa tgatttagca aaagttctaa gtgttagtcc agcatctgta agtgagatgc
     7801 tggctaaact tagatatgag gactatttag atataggatt tcaattaact gataaaggga
     7861 aaaaatttat tgacgattac aaaaaaggtt ttaagtaaaa gactttattt ttagggagga
     7921 taagaaaatg gctattggag tgattatgat aggacttacc ctaatagctg gatataattt
     7981 ttttaaatct ttctcggatt attttagaaa aggtgaatga tttagatgag tctattaatt
     8041 cttgtaggat tgctttttgt atcagttatt ttgctctttc tcattagttt agctctaagg
     8101 aatgagttca gtaatagacg ttaacgattg aaacttcaag tcctgtgtaa gataatatag
     8161 ttaattcatc ttattaggag gaagtaccca tagaaaccag agattatatt acaactcacc
     8221 aaaaattaat agaaagtgga ctaagccatt ttgaaaaaga aggatttcag caaactaaga
     8281 taaggcaact gtgcttgacc gcaggcgtaa ctacgggtgc tttttataaa cattttactt
     8341 ctaaggaagc gttatttgaa gaaattattc agccgcatat taatgcgctt attaatatgt
     8401 ataatgaaga gtttaccaaa tacctcttaa acttagaaaa tatagatata gagaaaagtt
     8461 ggagagagaa cttcaatgaa ttagatccat ttattaatta tatttttgag aataaacgta
     8521 tattcgattt ggttttattc caatcagctg gaaccaaata tcaagatatt gttgaaaaaa
     8581 ttacatcatt tgtgacaaaa aagacagtct gttgtctaga taagttaaag actatcggaa
     8641 tgatcaatca aaatttagtc tttaatgaag aagaaatcca cttctacatt tactcattct
     8701 acgctacatt ctatgatata ttgaagcata gctattcaaa agaaaagact atgctattaa
     8761 ccagaagatt gtatcaattt tctgttcctg ggtggattga attactgagt taattaaaaa
     8821 aaagcttgca ttgagaattg ttctcagtta tgctttttga taagatcgca ctgttatctg
     8881 aataacggtg catttttgtc agcttaagat aacagtgcga tcttattttt tggaggtgaa
     8941 agagtgtcaa aaatcaatcc gttaaccttg attactttaa atattttttt tccggtcatt
     9001 atttttttag gaaagggaaa tatatttcaa gtggggtgtt tcattattgc tacagtttta
     9061 gtattgatgt ttcgtaaaat aaagacatgt atttatttca tcttgattta ttcagttctg
     9121 tttttagtaa tgaaaattat tgatgctatc aagatagagt tttttactat attttttggg
     9181 acatccttat atattgtatt tcgaatgttg ccggtcgtaa tgatctcatg gttgttggtt
     9241 agtgtctata ctagcaatga attattatcc tctttagaaa aaatacattt accgaaaaaa
     9301 ataatgttaa gtattacagt agtgatcaga ttctttccga cttatcgttc tgaaataaga
     9361 atgattaaag aatctttaaa aatgagaaac attcgcttgt ctttttttca gcctctgcaa
     9421 tatttggaat ttctgttagt tcccgttctt atacgtgcaa ctatgattgc tgaagaaatg
     9481 accgctaatg caataacaaa ggggatagaa tctcctgtga aaagaagttc ttactataaa
     9541 acaaacatga gcttattaga ctatatcatc ctgttttccg ttactatttt tttcttaagt
     9601 attttgattt gaggtgaaag aattgtttga atttaacaat gtttcttttt catataagga
     9661 tgttaaggaa caagcgcttg ttgaccttaa tttttcaata aaaaaaggca gtcttacatt
     9721 gattactggt tcttctggga gtgggaaatc cacactatta aagttaatga atggacttat
     9781 tccaagactt tatgacggaa aattggttgg ggaaatttta tttgaaaata actcgctaca
     9841 atccttaaca caagaaagta tatcaaaaaa tattggctac gtaagtcaaa atcctagagg
     9901 acaattcttt acaacaaata caacgagtga gttagtattt tcgatggaaa actttggatt
     9961 tacaaaagag catatggata aaagaatcaa tgaattagct gaattattag atctttcaaa
    10021 tatattagat aaaaacattt tttcgctatc aagtggtgaa cggcagaaaa tttctattgg
    10081 atgtagttta tcgctaaact ctaaattaat tatattggat gaaccgtctt caaatttgga
    10141 cttcaaagcg actaagaaac ttgctgactt actttgtgat ttaaaaaata aaggatttac
    10201 tattgttata gctgaacatc gtttttacta tttgaaagaa ttaattgatc aagttctatt
    10261 tgttaaaaat agaactgtaa aaactacaac tattcagttg ttaaaaactg aaaacaataa
    10321 cgcattaagg aaatttgaca tttttaatag ttcgattgaa gttccctata tcgaaaaggt
    10381 aagtgcagat gtattaaaag ttgaagaaat tacttatgaa aatattctaa aagacataaa
    10441 ttttgatgtc aaagttggag atgttattgg tttggtaggg aaaaacggtg tggggaaaac
    10501 aaccttacta agaatactca tgaagattat tcgccctacc aaaggaaaga taatcgaaaa
    10561 taaaaaaaat ttgtcatcgc cgtttttagt aatgcaagaa atggattacc aatttttcac
    10621 agaatcagtg aggagcgagt tagccctggg gaatgaaata gttagtaaag atcagcaaga
    10681 aaaaatttta aaaaaaatgg aactttataa aatgaaagat caattaccgt ttgatttatc
    10741 cggtggagaa aaacaaagat tattggtttc tatagcttca ttgtcaaaaa ctaatttatt
    10801 tctatttgat gaacctacaa gtgggttgga ttatatcaat atggaaagga ttgctgacct
    10861 tattaaagac ttaaaagaaa agggtgcagt agttatagcc actcatgatc cggaatttct
    10921 ttataaaaca tgcaatagaa ttatttattt aaaaaatggt ggaataaaaa aagatataaa
    10981 acttagagta gaagattcaa ctttgataga acagattttt aatgacataa cgaaatagaa
    11041 ttgaggagaa tatatatgaa aacaaaagat tatattttta ttggtttagc tacagttatt
    11101 tccctagtaa tttacttttt ttcaataatg gttagtagta ttggtggggc gtttggtcac
    11161 agcatatccc cagggatttt tggattatta tctggaatca tatttgttta tattagctat
    11221 aactacccta gaaaaggaat ttttactatt tacacaattg ttttattgtt attttttacg
    11281 ttaatgggcg gggcgtactt accttggttt atcagttcga ttagtatggc gatcttagct
    11341 gatattattc tatctgtatt tggatatgat cgagcaattc ctcaagttgt gtcttgggtt
    11401 ctaatgcaac taggcagtgc tgccggacag tggattccca tatggttctt tacagatcgt
    11461 tttcgtcaag actggataga tagaggacaa tctgctgcaa caatggatgc aatgatccac
    11521 tacgcggtgg gtatatgggg aattatttca gtgttagttg tagcaagttt atctatgatt
    11581 ggtgtattga ttggaagaaa agtcttaaaa aaatacaaaa aatagaacgt agtttacctt
    11641 ctatctgttt gtacatctct aatttacgct attacggaaa gcattaacag ttgtaaacga
    11701 ataagagaag aaattgaatg cgggggagga aaaaatgaaa gtatacaaaa aattatttag
    11761 ttatgtccct aaaatgaaat actacggatg gttagccact ttattttctg atttttctgt
    11821 cgtattaaca gtctatggat attacagtat ttacaaattt cttcagtctc taattattgt
    11881 aggagatgaa tatttagcta agagttatgc ggttcaaact gttgcttggt taactttagg
    11941 agctttattt tatatttttt ctggcttatt ttctcatatc cttgggtttc gcttagagac
    12001 taatttgaga aaaaagggaa ttagcggttt aacagaagca agttttcgtt tttttgattt
    12061 aaattcttcg ggatatattc gaaagacaat tgatgacaac gcggctaaaa cacatatggc
    12121 aattgctcac ttgattccag atagtgctca agccatagta acacctgttt tatcagttgc
    12181 cttaggcttt ttcattaact ttaaaattgg attggttcta atcgccatgt tagtaattgg
    12241 tgtattcttg ttaaagaaaa tgacagagaa tacaaatttt attcaaaaat atcaagaatc
    12301 gttagatgtg ttaagctctg aaacggtaga atatatccgc ggaattcaag ttataaagat
    12361 atttggtgct aaagtaacat catttaaagc cttgaataaa gcaattaacg attatgctaa
    12421 gtttgcatat aagtattctt tgagtgggaa aacacctttt gttctttttc aatggctatt
    12481 ttttggcata gtttgcattt tgatagttcc tataaccttt tttataactt ctcttaccac
    12541 tcccaagatc ttagctgttg acttgttgat gttgtttttt ttaagtggaa ttatttttgt
    12601 ttctttcatg agaataatgt atgtaagtat gcacttgttt gaagcaagat atgcagtcga
    12661 aacactagac tccctttatt tagaaatgaa gcaagaccaa ctaaaatatg ggaacgagac
    12721 acagattaaa aattgtgaca ttgcatttga aaatgtcagc ttttcataca aagaagagtg
    12781 tgttttagaa aatttcaact tagttttaga acaaaaaaga atttatgcat tagtaggaaa
    12841 atcaggaagc ggaaagagta cgatagctaa actaatttca ggtttctaca atgtggataa
    12901 gggacagatt aaaattggtg gtaagccttt aaatcactat tcaaaagaca caattattaa
    12961 cacaattgct tttgtctttc aagatacgaa actattcaat cagagtattt acgataacgt
    13021 ttctatagca aatcctcaag cgccagcaag taaagtattt gacgcgctaa gattagccgg
    13081 ttgcgaatcg attttagaaa aatttcccga aagagagcat accttaattg gtagtaaagg
    13141 agtttatctg tcaggaggag aaaaacaacg tatagctatt gccagagcaa ttttaaaaga
    13201 tgcaaagatt gttattatgg atgaagccag tgcatctatt gatccagata atgagtatga
    13261 actacaaaag gcttttcaat atttgatgaa agaaaaaaca gttattatga tagctcatcg
    13321 attatccagc attaaatctg tagatgaaat tcttgtcttg tcagaaggaa aaatagtaga
    13381 acgtggaaat cataatgaat taatggctaa acatcgagtg tataaacaat tggtcactaa
    13441 ttatgagaat gcaaatgatt ggaggcttga caatgaaaaa tttttataaa aaaaaatttg
    13501 ctttaactga tcagggcgca gaagctttaa ctaaagcttc tatttctagt ttttttgtat
    13561 actgcattaa tatggtacca gcattcatta taatgatgct gatagacgaa ttagttttag
    13621 aaaatgcaaa gccccgttgg ctttattttg cagtatcgtt tgttactttg cttttcatgt
    13681 attggttgtt agatagggag tatgaaaact tatataatag tacttataaa gaaagtgcgc
    13741 atttaagagt gcaaattgca gacgatttgt caaatttacc attatcctat ttttcaaaac
    13801 ataatttatc agatttatct caaactatca tgtctgacgt tgaaggtatt gagcatgcga
    13861 tgagtcatgc aatacctaaa gccggcggta tggctctgtt tttccctttt atttcagtga
    13921 tgcttttggt tggtaatgtc aaaatgggat tagctgttat tttgccaacg ttatttagtt
    13981 ttgtcttaat cttgttatca aagaaatccc aaacgaaagc caatactaaa tattacgata
    14041 ctttgagaga aaactcggaa gaatttcaag aaactattga attgcagcaa gagattaata
    14101 gctttaatct atctaaaaaa gttcaagaca gacttttcaa aaaaatggaa gagagtgaaa
    14161 ggattcattt aaaggtagaa ttaagtactt tttcagtcat ggccttatcc tctattttct
    14221 catatgttag tttagcagta gtcattctag taggtgttca cttactgtta acgggtgaag
    14281 tgactatact ctacgtcgtt ggttacttac tagccgcaat aaaaataaag gattccttcg
    14341 attctatgaa agaagcagtg cttgaaatat tttatttagc tcctaaaata cagcgaatta
    14401 gagctatgaa ggaaaccagt atccaagagg gatcagatag tcctctgaaa tcttttgaca
    14461 ttgagttgcg agatgtctct ttctcatatg ataacaatac cccaatttta gatcatatct
    14521 cttttacggc taaacaagga gaagtgactg cgttagtagg tgctagtggt tccgggaaaa
    14581 ctagtatctt gaaattagtg tctagactgt atgattatga tgaaggctgt attttaattg
    14641 atgggtatga tattaaacgt gtatctccag cttctttgtt tagcaaaatc gctattgttt
    14701 ttcaagaggt aactttgttt aatacaagta ttttggaaaa tatacgtatt ggaaatagtc
    14761 aagcgtcaga tgaggaagtg aaaaaagcag ctcgtttagc taactgcgaa gactttattg
    14821 aaaaactacc tgacggttat catacattgg tcggagaaaa cggaagttca ctttctggag
    14881 gagagcgaca acgcctatcc atagcacggg cttttttgaa aaatgcgcca attttaattt
    14941 tagatgaaat tactgccagt ttagatgcgg aaaatgaaaa acgtattcag gaaagtttaa
    15001 atcgtttaat acaagataag acagtactaa ttatatcgca tcgcttgaag tctattgaaa
    15061 aggtaaataa aattgtagtg atggatcaag gaaaactagt tgatcaaggt actcataatg
    15121 agttgtatcg aaggtcagaa atatacaaaa atctcataaa gaaaactaaa ctatctgaga
    15181 aattcgtcta tgaaaaagaa gctcaatcac gatgatgaaa taggctttaa attaacctac
    15241 aatttttggg atatagttat aattcttttt caaaaagaat aattttatta aacagcatct
    15301 aattttgaat acacgaaaga atcagtatga ttgaattagt tcacttaatg tctattctta
    15361 taatataagg aataatcaaa gttttaaaaa attggcgata aaccagttaa acataaaaga
    15421 ttgaataaaa agttatagtt tctttcatat tgccgtttta agtagaggaa aagctactta
    15481 aaacggtatt tgtgtttgat tggactaata aaagtgaatt cttatttttc agagcctaaa
    15541 ctgcatattc ttttaaatga tttgattatc tctatgtacc gataaattcg ttttcaaata
    15601 gttatctatt catatgagtt ttatttcatg ttatttattg ttgacaagag caactcttgt
    15661 tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg aggtgtctaa atcaaaataa
    15721 aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg
    15781 cgatgattga aaagtatgca gataaacgtc aggaattaaa agccgcagga gatcgcaccg
    15841 ctctcgctaa attgccaaga gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg
    15901 atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc ccgtattaag tttagagaac
    15961 ttgcccatca agggttgatt cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat
    16021 catggcagta ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa gaactcatca
    16081 aactgtagta aaaccagaaa tttcatttga cgaagcgaat ggtgattata gacgtagcca
    16141 tcatgtatct ttaaaagggt actataatgg aaagaaagta attaaaggag cttcaaaata
    16201 gtttaatata taacttttag gagggaatca aaatggaaat tatttaccca cctttagttg
    16261 aagaaggatt aaagtattat cttgaaacga cccagcaatc gctagataaa tgcacttttt
    16321 atcgttcgat ggtagagaga ggaattatta ctgaaacggg tttaccgacc caacaggcaa
    16381 ttgaaaatgg attagtcaaa gattactatg aggaccaagg cttatcattt gatgattttt
    16441 taagaatcta tccaattttt gaagagtatg acgaggagtt atttcaatgt atcgatggtt
    16501 attgggagat acctgttgat atgaaagaaa atttagtttc acaattggaa tctggcgagt
    16561 taacttttga agatgcacaa caaattcaag catatcttga agatcgataa gtattgaagg
    16621 aggaagattt atgcgtcaaa atattatttt ggaatgtgtc gaaactggag aacgtctgta
    16681 tctcaccagc aaaaataaac ggaataaccc tgaacggtta gaattaaaaa agtattcgcc
    16741 aaaattgcgc agacgggcaa tttttaaaga agtgaaatag ctaagtggta aaaaggtgac
    16801 aaaatgggca taccagttac aatcgtttct gggtttttag gggctggaaa aacaacgcta
    16861 atcaatcaag ccttgcaagt ctcaccctac cccagggaag aaattattat aattgaaaat
    16921 gagtttggtg aagttgggat tgatcacaaa ttattagttc atagcgagga acgggtgtta
    16981 caactaaata atggatgtat gtgttgcagt cttcgcagtg atttattagc aatattgatt
    17041 tctttactgg aaacaataga agaacatcaa caaccaattt cacaaatcat cattgaaact
    17101 acaggtatag cagatcccca accaatcatt caaacacttt taacagcgcc tcaactaaga
    17161 ggaagagtat atatcgatag tttattgact gttattgata gtgacagttt tgaacttatt
    17221 gaaagagaac cacaagccct taagcaactt gtcatggcag atcgcatttt tgtgtctaga
    17281 aaacaaaact tgaatttatc tgcactaaaa aaattacaga gagaaatcaa aagtattaac
    17341 cctttatccg atattcgaat gttcacacat agtgaaacga ttcaaaaaaa ggatttttat
    17401 aatttgaaaa aatttaatca tcccttagtt tttgaaagtg attcatcaga aaatcactat
    17461 aatggcaaat actatcatca acaccatcaa gtacatgagt ttaaatcaat tctcttaact
    17521 tcagatcaag acataaaaaa agatttttta ctccaatgga ttgactggct gattttaacc
    17581 aaccaaggga gcctgtatcg ttttaaagga ttgattgctt tacaagagca ggattttata
    17641 gttgccatgc aagaggtaaa cgaacaggtt aattttcaat acaccactcg acaaaaagac
    17701 caaaccacta ttattctaat cggaaaaatg ttggatgaag aaaggatcaa acagagtttt
    17761 gataaattac tggcggaaag caaataatga taagtagtga ttgggggtaa tttctaatgg
    17821 agaaaacgga tatatctagt gcatatcgac gacttaaaag cccgaatatt aaaactagaa
    17881 aaagggcatt aaaaattatc aaagaacata aacgaaataa aatgaagaaa ttagcataat
    17941 taaggaagga aaaagaatga agaaaatcag tgtaggactt attgggatag cagctctagg
    18001 attattggga gcttgctcca gcactaatga tgcaaaagta tcgaatgata aagatggaaa
    18061 attagaaatt gtaacaacat tctatccaat gtatgatttc accaaaaaca tcgtcggcga
    18121 tgaagcgaac gtggatttaa tggttccagc gggatcagaa cctcatgact atgagccctc
    18181 agctaaggat atggcaaaag ctcatgatgc tgatgttttt gtatatcata acgaaaacat
    18241 ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta atgtagtaga
    18301 aggaacaaaa gacatgattt tattgccagg tagtgaagaa gaagatcatg atcacggaga
    18361 agaagatcac catcatgaac tagatcctca tacgtgggtt tcacccaaaa tggcaattaa
    18421 ggaagtttca aatatcaagg atcagttagt taaattgtat ccgaaaaaag caaaagtatt
    18481 tgagacaaat gctgaaaagt atttgactaa actgaaacgg ttggatgctg actataccac
    18541 tagtcttaaa gaagcgaaac aaaaaagttt tgtgacgcaa cacgcagcct ttggatattt
    18601 agctttggat tatggattga aacaagttcc tattgctgga ctaagtcctg aagaagagcc
    18661 atcttctggt cgattagctg aattaaaaga gtatgttaaa aaaaataaga tcaattatat
    18721 ttactttgaa aagaatgcta atgataaaat tgcaaaaact ttggccaacg aagctgggat
    18781 aaaattggaa gtactgaatc ctcttgaaag tcttacaaaa gaacaaatgg ataatggtga
    18841 agattatgtt tcagtaatgg aagacaatct aaaagcgtta gaaaaaacaa ctatggttgc
    18901 tggtgaagag gtagttccag aaaaagaagc gaaggatgaa aagacagttg ctaatgggta
    18961 ctttaaagat gccgatgtga aggatcgcga attgagtgat tatacaggcg aatggcaatc
    19021 tgtttatccg ttgttaaaag atggaatttt ggatgaagtt ttcgattaca aagcaaaaat
    19081 caataaagac atgacagctg ccgagtacaa agattattat actactggct ataagacaga
    19141 tattgatact atcaacatta aagacaacac cattgatttt gtagtcaacg gtgaacatca
    19201 tcaatacaca tacaaatatg ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg
    19261 tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt cgttttaaat atattcaatt
    19321 tagtgatcat ggaattgcac caagtaaggc agagcatttc catattttct ttggtggaga
    19381 aagtcaagaa aaactctata atgaattgca taattggcct actttctatc cagcttcatt
    19441 gtctaaacat gaaattgctc aagaaatgat ggctcattaa aattcaattt ttggaggaaa
    19501 tacatggtaa gaaaagtatt attgtctatg gattccaaga atattgatgc ttatcggcgt
    19561 tatcgatttg caaagcaaat gagattaaat gggagtacat tagctgatat tgctgcaact
    19621 ttacatgtta gtattgacac agttcataga gtattaaaac gggatcctga ttgttgtttt
    19681 atcaaggtca atactggaag aagtagacgt ttagcaaaac ggcataatag cttggccaat
    19741 taattgataa taccagagta gatcattttt cccttatttg gatttgatct gctttttatt
    19801 atgtcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca taaggtgttc
    19861 tccaatctaa acactttcta ggtctgttat tcagtttttc aatcatctga tttatcgttg
    19921 agttatctac cgtagctatc tctttcgatt ttggcaagta ttctcgaatg agtctattcg
    19981 tattttaatt tgttcattgt tgccaagggc atgaggatct ggaaaataaa agggcatatt
    20041 attcaatgct tctgtaactg cgaaatgatt ggagaattct tttcctctat ctggagtgat
    20101 cgttttgacc gcctcatttg gaatactgct taaaagttca atcattttct cgctcacata
    20161 agcggctttc tttctgggga ccttctttcc tgctaaaagg aagcatgatt ttctgtctgt
    20221 caaagtcact aaacaagtac caccagtttt gccagctaca atatcggctt tccaatgacc
    20281 aaaatcactt ctatcatctg cggattttgg acggccatgg atcgaatgag taaggcaaat
    20341 ctttccacga gactcgacat gattttttgt atgtcttgtt tttcctcgat ggtgcaatga
    20401 acggatcagg cctttattgc cgtgtgagag gggttctgta tcgaaaagat cattgcaaat
    20461 tgcacgttag atcgtgataa aactaattga aaagtccata tgttcatgct tcaaacgatt
    20521 cgagatttcc tctggcgacc actgaaaatc gacaaataaa tgacgaacaa ttgaccaaac
    20581 tcacggatca tttagaatgt gataacgtcc gcattttttt cttcgataag cataattttt
    20641 ttccgctttg gaaggtgaat agtttttttc ttgtttcttc tcagttcatg taaaatagta
    20701 aacgggctat gctgaatgtc gttgccaatt atactaatag aaaatccttc ttcgtgaagc
    20761 agaaagattc tttctcgttc gagtatagta agatgacgat actggctcat aattattttc
    20821 cttctaggag tgttctcgac aaacttatac tagttggata tcgattatga ctcattttta
    20881 tgtgttgcac ttaaagtgta aattcaaggt atagaaagag gttccctatg aaaaaaatac
    20941 ctattacaat cttaactggc tatcttggtt caggaaaaac gatacttcta aatcatattc
    21001 tgtacggtga tcacggaaag gaaattgctg tcattgtaaa tgacataggg gaagtaaatg
    21061 ttgatggggt attagtcgac aagaaaggtt ttaaaagaac tggagaaaaa cttgtttcga
    21121 tgccgaatgg atgcatttgt tgtacattac gcgaagattt gattgttgaa ctagatcaat
    21181 tagtgcaatt ttctgacttg aatcaaattg ttattgaagc ttcaggaatc agcaaaccaa
    21241 ttccaattat tcaagcaatt atattagcta aaaacccttt agacattgat ttgacccaac
    21301 gattagagat tggtgccatt attatagtag tggatgtaaa acgtttttgg gataatttcg
    21361 aaacgggtga aaagattcag gaaagaaaag ctgaaggtaa tgtagttgaa gaagaacagg
    21421 atattcgtgg cttgttaatt gatcaattcg agtgttgtaa catattactt ttaaataata
    21481 gtgacttagt gtcaaccaag acgatttaca aaattaacgg tctaattgct aagttacagt
    21541 ccaatgccaa aataattagg acaattaaag gtgaagtaga cttatcgaaa attctaaata
    21601 caaatctttt taatatagat gagacttcct attcagtggg ctggttcaaa gagttagagc
    21661 ttggctatca aaatcatatt cctgaaacag aagaatatgg tatctcaagt tttatctatc
    21721 gaaatcggtt actccttgac gcacaaaaat ttgcccatct tacggataaa aattttcctg
    21781 gcaatattac aagagcaaaa gggtttcttt ggtttgccca acatagtgat ttttcgacga
    21841 tctttgaaac atcgggtagc tataaaagta ttgaatcatt aaattattgg gtagctagtt
    21901 tacctaaaat tgagtgtcaa atgattatac aaaaaaatcc cgaattgaag gaagcctggc
    21961 atccgttgtt tggtgaacgc attaataaac tagtttttat tggtatgaag atggatcaac
    22021 aatgtattaa ggaacaactt gagggttgtt taactgattc tcttaatgca gataatttcc
    22081 gaaaaatttc accatttcca tggaaaaagt ctaatatgtt tagccagata tcgttaatga
    22141 aatttttaag aaaagctggt gtcaagatag gcgatgtcat aattattggc attttgatta
    22201 ttacctcttt tattccctta tttgtattct ctatagaaaa ggagaatgaa acaactgaag
    22261 atgctatcaa atatgccatc gttcgaattg atggcaagga aatggatcgc ttcgatttgg
    22321 ataagatcaa tcataaactc gtgaattatc atcctgcgaa gggacaatat aatattgttg
    22381 agattaaaga tggaagaatt cgagttaaag aagataatag tcctgatcaa atcgcggtta
    22441 gaactggatg gatctcaaaa cccggtcaaa cgagtatttg tttaccccat aagttagtta
    22501 ttagtattga gaaaaaagaa agcacagatt actacattta ctaatctgga aatagatgag
    22561 gaggttttta ataagaaaaa gatattatta ttaataatag caggggcttt attaagtgtg
    22621 ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag gcacaagtat ctttaacggg
    22681 acctgcaatg gtttagttaa cagtcggtta gattacttat tttggctctt cgtcaatgag
    22741 tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac agctatcttg
    22801 tctagccgtg ttgttgttct ggctttagtc agttgggtag tattcttagt tttcttaaaa
    22861 tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct atagccatag
    22921 gcaattcgtt ttagtacttt gatatggtta ttcaaacatt ctatagggcc gttggaatag
    22981 ggaacgcgca acgctcgttt gatttctgtt tggtatttct tgaaggtcgt gaacactggt
    23041 tggtagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca
    23101 tcttgtcttc tgataacgga aagaaaatcc tggtagactt cgtagcctcg ctttaaggaa
    23161 tcatcataag ataatagccg atcgactatc tctgcttctg tcagatgatc tcgaaatgac
    23221 ggacgccagt agcgatgctg acctttcaat tcccacgcat tcttttgaag tagtttccag
    23281 tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat tacttgaatt
    23341 cggtggttgg taaaagcccg accaatgtgt tggctaatat gaaagcgatc ggcgacgatt
    23401 cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc
    23461 actagacgca cttgttcacg gacttttata gggtagcgca agaaatagtc gcgtaaagga
    23521 ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc catcatgaca
    23581 aagctcatgg ctcccgagac atttttcact gatttgaatt catcgaaaca caccacttct
    23641 ggtaacagct ggctcaaaga aatggtttta ggccgaaggc tgcgaagtat tcgaatgaca
    23701 gtggtcggag aaatgtgctt catacgtgcg atcaaagaca tagaaagagg ttcgcttaat
    23761 agctcaagaa tggcttgttt gactctgcga gcaatggagc agcgacgatc actaactgaa
    23821 tcttctgcta aaaaagtctt tccacagtgt ttacatttga agcgctgttt gttaatccgc
    23881 agaatggtct tgtagttact gacatcattc aataagatgg atactttttt ccagccctat
    23941 ttaatgatcg tttgttcatt ttgatagtgg cgatgaaagc attcctccgg ttgataggtc
    24001 aatgtgccat caaaaacaaa acatgtttcg tcattgattc gctctttgcg aaaggcatct
    24061 tcatgaaaga taagatttag gtctagaata tctagtaact ctttggtaaa ctctgaatag
    24121 gacatcttga aacactcctt tgaatggttt tcgtcgactt tattctaaag gattttaagg
    24181 tgtctgtttg cgtttattag aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca
    24241 ctagatatta tagatccctt attttcttcg cttcaaattg tcaaggagtc atcataaatt
    24301 ttgtatggat tcttttgttt gggtagtagt cttctaaata atcttggatt aacaccttca
    24361 tctctatttt acttttatat ttgttggagt cgattaattc tcatttgatt gatcgatgaa
    24421 acgcttcaat tactgcattg tcaacaggag tcccagtttg gctcactgaa tgtgtgatgt
    24481 ggtggtcgtt caacagctct tgataaataa aagaccgatg ctggctacct ctatcggaat
    24541 gaattaccgt aggcaattct ccttaataaa tttgtaatgg acggacgaca atctcttttt
    24601 tcatgtgtga atctaccata tagtaacgta gaatacgagt tgctaagtca atataggtgg
    24661 acaaatagag gcgaccgtca ttacatggaa tgtaagtaat atccatcacc caaacagcat
    24721 caatttggtt ctgttcaaat tatcgattca acattttttt accatttctt tgattgtttt
    24781 ttctctacca tgagaagatt tgcgtcgatg aaatcctctt gtgcataaat tcatttcacg
    24841 cataagaaca gctacaacac gtttattgat ttcgattcct tcatcgtaaa gtttttgagt
    24901 gatccctgga gatccgtatc gatctttgtg ttcgtagaac actcgtcttc ctcttttagt
    24961 cgtttatttt cctgttcaag aatgtagaaa tctgctttat caggtaatct ttttccttgt
    25021 ccagcaaaag catttttccc gtatcgacga tattctgaaa cccatcggga aattgttgga
    25081 cgagagatat tgtattcttc agcaattttc ttggtagtta cttttttctc aaggatatcc
    25141 tgtgaaatct ttagtttaaa tttacgatca tatgtttttc tcaatagagt tcattccttc
    25201 cggtaacgta actgatagtc aagaaaagtg tatcatagcc caacttagta tcagggtttg
    25261 tcaactaaac tgtggaagtt gatagataag agtttttaac aaccaaaatt ctcttgacta
    25321 tttttgaaat cagacaaatt ttgattggac acatagttga ataaatctac cgtcgttacg
    25381 gaaggtaaat cgcatgcttt tcaattctag aggagaagtc ctcgttgaag taccatagcc
    25441 cagttctgga ttcgacctcc tgcccacttc atacgtaaat aaagtaattt taggagtgag
    25501 actgtgacaa aagttatttt taagctaaaa acgaattaaa aaacgttgat aattaaaatc
    25561 attcgtttta gattctatga atcattatag aacgctgtaa aatcgatact ttataattat
    25621 aaagaagata gtttcaaatt tattgttcga aatttgaaac tatcttttta cttatgtcac
    25681 agactccctt agttgagtta ttctttaatt tcgaatttcg agatagctag atatattgca
    25741 agcatacatt cttgataaca atcttgatca tattttccag ttctccagga ttgcttatga
    25801 atcaaagagc taaatctttt gagaattgcc tcaacagctt cttcatttcc agcctttgct
    25861 aaaatgaaat cttcttttaa agaattcata aatctacctt cttcccatgg acttataatc
    25921 tatttctttg ggaaggagga gaacgagcaa agaagcatac aaaattagtt attatgcaga
    25981 tattaaacca gcattttttg aatttttttg taaagtcgat gttttaaatt tgtcgcaccc
    26041 tgtcttgtaa tccccagaac ctttccaatt tctttatcag ttttatcaaa gataaatttt
    26101 tcgataagaa aaatttgttc aggttctttc aacaaaggaa ggatttttgc taattttata
    26161 tcttcaacaa ggagagtcac atttaaaaca ttaatcggtt gtttaaattt tatagtatct
    26221 ttagtaaaca aatatggtgg gagttgatca agttgttcat gtttttgaat ttcttcttgt
    26281 tttttataat aagtattagc ggtgttcaaa atgacctttt caaagaatcg aatcaactgg
    26341 gcatttacgc gttcgtcgtt atctgacata ttgcacacct cctccattaa gatttaaagg
    26401 gaaaagaggc aaaaaacacg aaaagaagct aatgcaatta tgctgcgctg cgaataactt
    26461 ctataagctc acatgcattg ataggactgc tgatgtttat tttcaaaaat gtttttttag
    26521 ttgataatgg atagttatct ttatacttag aaaagaaaat tcatatatct tcttaaatat
    26581 caaacatgtc cttatggcta agaaaatgtc ttgagctttt tacgtataat aaatcttttc
    26641 taaaacctcc ttttggataa tattcttatt cgtgtataga ttgtattttt tatttcttcc
    26701 cccttcataa ttaacaacta gaaaagagtt cgaatagaca cgtatttgaa cgaaaatttt
    26761 tattggattc ccaaattgga atatttagaa acgaaaaatt cctattatct gtatagtgct
    26821 tagataataa cggatcataa taattattgt ctttgaacta gatatatgta aatagtattc
    26881 atcatgatga aaaatgatga gttctcggtg ataaaatgta ctttttacgg tggaatatac
    26941 atgaaagcga taagtggata ttttataatg aagtaagtta gcttattttg acagaggaat
    27001 agtattttag ataacgtata aaatactatt taatgatgta gaaaggaaag agagaaacaa
    27061 aagtgttttc agataaagaa gtcttattaa agtctcttca tgtttgttta aagttaccta
    27121 tcgtggtttt taattccgaa ttcagtatta taaaagagta tcgctcagat agaactactt
    27181 atttttttta tgattataaa aagttactaa ctgataatat taaaaaaact aaagatttcc
    27241 atttctttac tggtgatttt aatgagatgt ttcttctata catgtttaaa accagatatt
    27301 acttatttgg tccatttaga gcaaataata ttgataaagt tttttttaaa ctaaaaatga
    27361 acaatttgaa tgttgcaatg gctgatagag aacgactcta taattcattg caaaatttga
    27421 ctttgtattc tttaggagac attcgagata ttctgatttt agttcattat ttttttactg
    27481 gaaaaataga agatctattc catgaaccgt taattgaata tacagaaaat ctaagtaaaa
    27541 ctatcgagca aatcaaaata gataatctgt tatcacaaaa ttacgatcca gaaatatatc
    27601 tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt cgaaatttag
    27661 aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga gatacgattc
    27721 ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg tctataacat
    27781 taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa gaaaacgaat
    27841 tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt tattatacta
    27901 aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct gtcgttcaat
    27961 tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat tatttttctg
    28021 taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg atttataatt
    28081 atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac catactatca
    28141 gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga gtattcaaaa
    28201 agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat ttcaacaaca
    28261 ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt ggaggagttg
    28321 aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag ttgtttcgta
    28381 gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga caaagtagta
    28441 attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca tattaatggt
    28501 agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat atcaagttat
    28561 ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca gttaattgaa
    28621 agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt aaacaactac
    28681 aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta gagtactaga
    28741 cactttaaat atggacaatt caattataaa gaaaaagaat caataattta atgaggctgt
    28801 gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata actccaatca
    28861 ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt tataattata
    28921 aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac ttatgtcaca
    28981 gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac tctgaagttt
    29041 aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg aacttaattt
    29101 cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt taaaaagcac
    29161 aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc atgaaattct
    29221 ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata atacgtatca
    29281 gatttatctt ttattcttac taggaaagta attattaagg gagttgattt gaataatggt
    29341 tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat tgaagtacag
    29401 tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc ttgtcttggg
    29461 aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac ttgaaacagc
    29521 gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc aggagaatcc
    29581 tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag aactaactgg
    29641 agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata atattgcagc
    29701 atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta aatatgtaga
    29761 aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct ttgtaagaat
    29821 taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc gtgcaagtga
    29881 tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg gggtaattac
    29941 gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc tcaagaccga
    30001 tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa ttgtaccggg
    30061 tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata gcttagatgt
    30121 atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat atgagaaacc
    30181 taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta taatcttaga
    30241 tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg gcacgccttg
    30301 gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta tacagatgca
    30361 catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa caaaagtagc
    30421 tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt caataactaa
    30481 taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag tcgttattcg
    30541 atatgtttta caattgacta agccattaga tgaaatctta gaagatatga aagcaacagc
    30601 acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt ggttgtcgga
    30661 tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt tgcaagatat
    30721 tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc catatatcgg
    30781 gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga aagtattcat
    30841 ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag gtactgtatt
    30901 agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag atactgtaga
    30961 atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat taaaaatcca
    31021 aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta aggaatcacc
    31081 aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta aagaatcaat
    31141 tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag agcctgatac
    31201 ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt
    31261 cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg atgcaacata
    31321 cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta catttgataa
    31381 accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata ataaggactt
    31441 cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag ctaaaggtgt
    31501 aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc aagatggttc
    31561 tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa atccaggaga
    31621 aaacattcct gcaggctacc acaaagtaac cttcaccgca gg
//
LOCUS       DACEJR010000033        30538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEJR010000033 DACEJR010000000
VERSION     DACEJR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.033"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            233..475
                     /locus_tag="IW651_001511"
     CDS             233..475
                     /locus_tag="IW651_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0814240.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="IW651_001512"
     CDS             472..942
                     /locus_tag="IW651_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0814241.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1070..2284
                     /locus_tag="IW651_001513"
     CDS             1070..2284
                     /locus_tag="IW651_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0814242.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2994..3182)
                     /locus_tag="IW651_001514"
     CDS             complement(2994..3182)
                     /locus_tag="IW651_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814243.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3273..3482
                     /locus_tag="IW651_001515"
     CDS             3273..3482
                     /locus_tag="IW651_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0814244.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3594..5207
                     /locus_tag="IW651_001516"
     CDS             3594..5207
                     /locus_tag="IW651_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0814245.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5242..6453
                     /locus_tag="IW651_001517"
                     /pseudo
     CDS             5242..6453
                     /locus_tag="IW651_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6476..7402
                     /gene="glsA"
                     /locus_tag="IW651_001518"
     CDS             6476..7402
                     /gene="glsA"
                     /locus_tag="IW651_001518"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0814246.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7610..9070
                     /locus_tag="IW651_001519"
     CDS             7610..9070
                     /locus_tag="IW651_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814247.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9291..11054
                     /locus_tag="IW651_001520"
     CDS             9291..11054
                     /locus_tag="IW651_001520"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0814248.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11138..11596
                     /locus_tag="IW651_001521"
     CDS             11138..11596
                     /locus_tag="IW651_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0814249.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11705..12388
                     /locus_tag="IW651_001522"
     CDS             11705..12388
                     /locus_tag="IW651_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0814250.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12535..13149
                     /locus_tag="IW651_001523"
     CDS             12535..13149
                     /locus_tag="IW651_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0814251.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13165..13371
                     /locus_tag="IW651_001524"
     CDS             13165..13371
                     /locus_tag="IW651_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814252.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13912..15042
                     /locus_tag="IW651_001525"
     CDS             13912..15042
                     /locus_tag="IW651_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0814253.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15134..15889
                     /locus_tag="IW651_001526"
     CDS             15134..15889
                     /locus_tag="IW651_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0814254.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15947..17986
                     /locus_tag="IW651_001527"
     CDS             15947..17986
                     /locus_tag="IW651_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0814255.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18095..19246)
                     /locus_tag="IW651_001528"
     CDS             complement(18095..19246)
                     /locus_tag="IW651_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0814256.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19400..19930)
                     /locus_tag="IW651_001529"
     CDS             complement(19400..19930)
                     /locus_tag="IW651_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814257.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20029..20379)
                     /locus_tag="IW651_001530"
     CDS             complement(20029..20379)
                     /locus_tag="IW651_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0814258.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="IW651_001531"
     CDS             complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="IW651_001531"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0814259.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="IW651_001532"
     CDS             complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="IW651_001532"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0814260.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22782..23444)
                     /locus_tag="IW651_001533"
     CDS             complement(22782..23444)
                     /locus_tag="IW651_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0814261.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23628..25274
                     /locus_tag="IW651_001534"
     CDS             23628..25274
                     /locus_tag="IW651_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0814262.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25265..26347
                     /gene="uxuA"
                     /locus_tag="IW651_001535"
     CDS             25265..26347
                     /gene="uxuA"
                     /locus_tag="IW651_001535"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0814263.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYIFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26455..28008)
                     /locus_tag="IW651_001536"
                     /pseudo
     CDS             complement(26455..28008)
                     /locus_tag="IW651_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            28156..29297
                     /locus_tag="IW651_001537"
                     /pseudo
     CDS             28156..29297
                     /locus_tag="IW651_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29423..30502)
                     /locus_tag="IW651_001538"
     CDS             complement(29423..30502)
                     /locus_tag="IW651_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0814264.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
BASE COUNT        10504 a         5226 c         5979 g         8829 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
      961 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     1021 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     1081 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     1141 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     1201 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     1261 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     1321 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     1381 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     1441 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     1501 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     1561 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     1621 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
     1681 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     1741 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     1801 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     1861 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     1921 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     1981 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     2041 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     2101 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     2161 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     2221 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     2281 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     2341 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     2401 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     2461 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     2521 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     2581 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     2641 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     2701 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     2761 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     2821 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     2881 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     2941 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     3001 ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc cgttgaaaca
     3061 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     3121 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     3181 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     3241 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     3301 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     3361 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     3421 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     3481 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     3541 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     3601 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     3661 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     3721 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     3781 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     3841 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     3901 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     3961 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     4021 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     4081 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     4141 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     4201 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     4261 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     4321 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     4381 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     4441 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     4501 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     4561 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     4621 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     4681 actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag tattgggcta
     4741 aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct ggcaatgcag
     4801 agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag aacagacaaa
     4861 atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag aagataacag
     4921 agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa ctggaacagc
     4981 agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata ggtgctgaaa
     5041 agtggattga gttaatcaag gaatattccg taccaaagga actgacagca ccattattaa
     5101 atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat aacgaaagaa
     5161 tacaggaaat tgagatatat taccgattta ttggaagagt tgagtaatca acaagagtaa
     5221 tatttttaac taagggaaac cggcggtgtc tatacttggg tcagtcgcac attgggaaaa
     5281 cgattcggtt ttgccgcaat ctttttccaa tggtttgaga taacagttgg ctacttgacc
     5341 atgctgtatt tcttaactgg tgcattatcc tatgcaacgg gtattggtgc tatacaaaac
     5401 aataaatatc tgaaattggc aatcctattg attattttct gggctatttt actctcacag
     5461 ttaaaaggaa ccaaatacac ttcattgatt gctcggatcg gttttgtagc tggtatcttg
     5521 ttgccagcat tagtcttgtt tggcttaggg atccattatg tcgcttcagg cgctccacta
     5581 caaacgatac tgtctatgaa aacattgatt cctgattttt cgaaattgcc tacgctggtt
     5641 gtttttgttt cctttatttt agcatatatg ggcgtagaaa catctgccag tcatgcaaat
     5701 gaaatggaaa accctaaaaa ggattatcca ttagcaatgt ttgtattggt tattctggca
     5761 atcattttag atacttttgg cggattgacc gtcgctacta cagtacctca acatggtttg
     5821 tctcttaata caggggtgat tcaatcgtta gaattcctat taagacacat tgatccaaat
     5881 cttgcatgga ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt
     5941 gcttcttggg taacctctcc ttcaaaagca cttcacgtag cagcagaaga aggattactt
     6001 cctgaatatt ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat gatcgcaaat
     6061 gggatcgttg caacaatctg ggcagccgtc tttacattaa gcggcggggg gaataatctt
     6121 tcatttcttg cagcgatgtc attgactgtt gtaatctacc tgatgttgta ctttatgttt
     6181 ttcgcgggct atttgaagct ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt
     6241 ccaggcggaa aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac ttcacttttt
     6301 gctttttgta tctcatttgt tccacctgct agtatcgcag ctggacagca tgggatttat
     6361 gaaacgattc tgattgtttc ttatcttatc gtcttagtta ttccatttac gatctacgca
     6421 tgccgtcata aattccagca tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga
     6481 agtaaaagaa ttagaacaat tgattggaaa aaatttccac tattatcaaa caggacaagt
     6541 ggcggattac attccagcat tagctgaagt ggatcccaaa caattaggta tggcgattta
     6601 tgatttggat acaaaacaac tgattgaggg aggagacgcc gatgtgcgct ttgccatcga
     6661 aagcatgtca aaagttcccg tattgttatt agcaatcaaa gacaacggaa tagaaaaagt
     6721 ctttcaaaca atcaacacgg aaccaaccgg ttttgccttc aattctatca tgaatatgga
     6781 aatcaatcat cgcaaacatc caatgaatcc ttttgttaat gcaggagcga ttgcaacaac
     6841 ctcgttgatt gccggtaaag atgcagatga aaaatttgag cgaatcttgt catttatgaa
     6901 agagatctgt gatgatccta atatcacatt gaatgaagaa atctatcatt ctgaatcaag
     6961 aacaggtgat atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg
     7021 agacgtaccg gagatactcg atgcttattt cagacaatgc tccctcaatg tcaccgctac
     7081 aggcatagca aaattagctg ctgtgctggc aaataaagga attgctcctt ggaatggcaa
     7141 acaaatcatt actgaagaaa gtgctacaat cgtcaaatca attatgacaa ctgctggttt
     7201 gtatgatgaa tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg
     7261 cggcgggttg atggcagctg tgccaaatcg ttacggcata ggtgtcttca gcccggcact
     7321 agatccattt ggtaacagcg cagctggtat ccaattgttg aaagatgtag tcaaagaact
     7381 agacgctgac atctttgaat aaccattctt gaaagaggct ggggtaaaag tccaaacggc
     7441 tcaaagctac ctacaatgag gctgaaattg tgagcttgtt tgacttttgt cccagtttct
     7501 ttttttattg gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa tacgatttac
     7561 agatatgatt aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa
     7621 ttacatcgag cgaaatatat ttagaaaagt gtatctgtgt gaacaattat ttgaattcca
     7681 agaaatagac atcgaacaaa cagctatttc tctaagggtg actacaccaa cgatccttca
     7741 cgatctagaa agtctagccg agtgtctaga atattgtata aaagaacaag taagagagaa
     7801 gcataagtat aaactcgtat tcaaacatgg tattgctctt tctgagctca cacagtttct
     7861 gtacggtcaa tcttattttt taaagttttt aagttattat tttaatggta tattcacgag
     7921 tacagaactg gctgatcttg aatttatttc tttgagtaaa gtatatacaa tcaaaaaaat
     7981 cgtattggat ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga
     8041 atttgactcg cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga
     8101 aaataaaaat ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca
     8161 tttttttaaa agaagatatt cggaagatga aaagtccttt atcatacgag aaatagagat
     8221 cgcaatcgga agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga
     8281 aacgaagccg ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga
     8341 cttacaagaa gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa
     8401 tgagaacatg gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa
     8461 tgacctatca ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc
     8521 agaaaatttt attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg
     8581 acaagtattt ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg
     8641 aaaaattgta gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa
     8701 tgaaaatttc attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg
     8761 gagacaggaa atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg
     8821 aacccaattt catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg
     8881 ttcattagaa gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga
     8941 tgctgctagc tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt
     9001 aaaaggtgca aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca
     9061 tatttcttga tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga
     9121 aggtttttat ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga
     9181 aattttttta aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt
     9241 aacaaatcta ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag
     9301 tattacatgt attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg
     9361 aaatgaaaca gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag
     9421 gtgggcacgt tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat
     9481 ttgattcacc aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc
     9541 ttttaactgg tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt
     9601 ccaaccaaca tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca
     9661 gcttagcaac aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag
     9721 catttatcgg tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg
     9781 gtgttttaca atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga
     9841 atcatggcag tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca
     9901 ataacctatt taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg
     9961 aaacggtgat ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg
    10021 tccatacgga aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt
    10081 ggcatgtacc atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat
    10141 ttaatatgat catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg
    10201 ccgcagtcaa tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc
    10261 cagatcatta ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt
    10321 tggcttctca gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat
    10381 atgatcaatt atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg
    10441 gcggaacgat ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat
    10501 caagtatccc gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc
    10561 tagattgggc gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg
    10621 tagaacatgg tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata
    10681 atggaggaaa agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag
    10741 taaaagcttt tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag
    10801 aaaatgacgt agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag
    10861 aagatggtag tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt
    10921 caatgaacgt attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc
    10981 agctttatga acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct
    11041 tgaatattca ataattcatt atgctcacca tccagataat tgtttatact agtatctgga
    11101 tggttttttc attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata
    11161 tgtcaaaaag ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc
    11221 atttggggat acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa
    11281 gactggtaca tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt
    11341 tggtcaatta gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat
    11401 aaatgtgacc atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga
    11461 aggggatcgg acattagctt attggcggaa agtccatgct gagtttttta aacatgctta
    11521 taaagaagcg ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga
    11581 agtaattttc ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga
    11641 atagaaaagg gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga
    11701 tattgtggat acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga
    11761 aaacgagccg ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg
    11821 cggagcagtt ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt
    11881 tgatttaccg gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac
    11941 tgcgacagaa aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga
    12001 tgcaacttct cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca
    12061 cgaacacggt cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca
    12121 agaagatggt tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa
    12181 gacgcagaca cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt
    12241 aattatggaa ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc
    12301 tttttcagtc gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt
    12361 cgaagccgca agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa
    12421 agaaaaattg atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc
    12481 attttgtctt caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt
    12541 gaacggagtc cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc
    12601 atccctagac cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca
    12661 cccttcagtt acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag
    12721 agaaaacaag atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt
    12781 atccatatcc ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct
    12841 aatgaggaaa gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca
    12901 gtaccagcat tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag
    12961 atacggaata aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat
    13021 ttaaatgaaa cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct
    13081 gtaagccgtt tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt
    13141 cccgaataaa aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca
    13201 aaagaaatga aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa
    13261 gttccgccat tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata
    13321 gaaatgccag atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt
    13381 ccgaaaagtg aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct
    13441 ggtgcaactg aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa
    13501 aaatattaaa gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag
    13561 gaaaagtcta tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc
    13621 tatacaaaca taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac
    13681 tgttggatta aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa
    13741 aactcggacg atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt
    13801 tctttatatt tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt
    13861 ggattgctgt gtgaggagga gatgcggtga aaaaagatcg attggtggat tttgggtcct
    13921 tatttgactt tgtccatgat cggtctttta gaagtataca gtgccagttc ttatcgttta
    13981 ttgcaagccg atgaaaatac aaaaagtcta ttattgcgtc aactaatttt catatttttg
    14041 agttggagcg tgatcttctt agctcgttca gtcaaactac actatttact tcaccctaaa
    14101 atagcaggat acggtttagc cttatcgatt ttctttttag tattagtaag aatagggata
    14161 ttcggtgtca ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat tcaattccag
    14221 ccttctgaac tggcaaatct ttttttgatt ttttatttaa gctggttttt tcgtgacgga
    14281 aatagtagcc caaaagatct aaaaaaacca ttcctgatta cagtaggtat aacttttctg
    14341 attttatttc agccaaagat tgctggagca ttgatgatcc tttcgattgc gtgggtcata
    14401 ttttgggcag cggcggttcc atttaaaaaa gggatctatc taatcgttac tttttctgca
    14461 ttgctgattg gagcagcagg cggggtatta tatttaggaa ataaaggttg gcttccacag
    14521 atgtttaatc atgcttatga aaggatcgcg actttaagag attcttttat cgatagtcat
    14581 ggtgcgggct atcaaatgac ccattcgttt tatgcactat ataatggtgg catatttggg
    14641 cggggattag gaaatagtat cacaaaaaaa gggtatcttc cagagacaga aacagatttt
    14701 attttttcga ttattacaga agagcttggg ctaatcggtg cattgtgtgt gcttttctta
    14761 ttgttctcat tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa tcagcaagct
    14821 ggattgtttc ttttaggatt cggaactttg ctattcgttc agacgataat gaacgtggga
    14881 agtatcgcgg ggctgatgcc aatgactggt gtgccattgc catttgttag ctacggagga
    14941 acaagttatc tgatcctttc tttaggaatc ggaatcacgt tgaatatttc ttcaaagata
    15001 caggccgaag agttggcgct ctatcgacct gaaaaacaat aaagagaaac tgagaaactc
    15061 tcagtttgaa ttatcataaa gtacaatctt ttatttttca agcgagaggt tcttttagaa
    15121 agaggaattc gcaatgaaag tatctcaaaa aatcatatta tctattttgt tactgcttac
    15181 attagtagtt ggctattttt cgattgagtt tatccatgga ttttcgtctg caaaacagag
    15241 ttcaactgtg aaaaaggtag agccgaaaag tatccctacc acactaaatg tggctttgat
    15301 tggttcagat gctcgttcga aagacgaaaa cggtcgctcg gattcacttc tggttgcaca
    15361 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
    15421 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
    15481 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
    15541 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
    15601 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
    15661 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
    15721 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
    15781 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
    15841 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
    15901 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
    15961 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
    16021 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
    16081 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
    16141 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
    16201 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
    16261 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
    16321 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
    16381 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
    16441 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
    16501 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
    16561 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
    16621 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
    16681 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
    16741 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
    16801 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
    16861 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
    16921 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
    16981 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
    17041 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
    17101 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
    17161 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
    17221 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
    17281 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttatcaag caacgcgtgt
    17341 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
    17401 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
    17461 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
    17521 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
    17581 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
    17641 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
    17701 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
    17761 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
    17821 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
    17881 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
    17941 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
    18001 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
    18061 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
    18121 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
    18181 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
    18241 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
    18301 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
    18361 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
    18421 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
    18481 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
    18541 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
    18601 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
    18661 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
    18721 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
    18781 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
    18841 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
    18901 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
    18961 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
    19021 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
    19081 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
    19141 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
    19201 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
    19261 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
    19321 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
    19381 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
    19441 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
    19501 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
    19561 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
    19621 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
    19681 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
    19741 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
    19801 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
    19861 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
    19921 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
    19981 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
    20041 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
    20101 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
    20161 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
    20221 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
    20281 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
    20341 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
    20401 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
    20461 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
    20521 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
    20581 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
    20641 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
    20701 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
    20761 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
    20821 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
    20881 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
    20941 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
    21001 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
    21061 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
    21121 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
    21181 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
    21241 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
    21301 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
    21361 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
    21421 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
    21481 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
    21541 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
    21601 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
    21661 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
    21721 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
    21781 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
    21841 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
    21901 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
    21961 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
    22021 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
    22081 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
    22141 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
    22201 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
    22261 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
    22321 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
    22381 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
    22441 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
    22501 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
    22561 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
    22621 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
    22681 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
    22741 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
    22801 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
    22861 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
    22921 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
    22981 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
    23041 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
    23101 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
    23161 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
    23221 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
    23281 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
    23341 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
    23401 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
    23461 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
    23521 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
    23581 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
    23641 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
    23701 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
    23761 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
    23821 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
    23881 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
    23941 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
    24001 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
    24061 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
    24121 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata
    24181 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
    24241 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
    24301 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
    24361 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
    24421 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
    24481 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
    24541 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
    24601 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
    24661 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
    24721 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
    24781 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc
    24841 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    24901 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    24961 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    25021 cagacccact attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag
    25081 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    25141 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    25201 caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    25261 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    25321 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    25381 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    25441 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    25501 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    25561 agaagtcatc tgctatattt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    25621 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    25681 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga
    25741 acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    25801 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    25861 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg
    25921 aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa
    25981 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    26041 tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    26101 tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat
    26161 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    26221 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    26281 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    26341 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    26401 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    26461 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    26521 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    26581 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    26641 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    26701 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    26761 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    26821 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    26881 ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat
    26941 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    27001 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    27061 agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    27121 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    27181 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    27241 atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    27301 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    27361 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    27421 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    27481 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    27541 gccattatta gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt
    27601 cattttcgta tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga
    27661 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    27721 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    27781 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    27841 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    27901 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    27961 gctttttttc ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    28021 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    28081 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    28141 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    28201 ttatcgctta ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca
    28261 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    28321 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    28381 tcgggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact
    28441 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    28501 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    28561 gatggactac cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa
    28621 gattcagcca tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat
    28681 gaaaataagc cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata
    28741 gaacctggag aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct
    28801 gaaataaccc ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac
    28861 caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca
    28921 gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac
    28981 caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt
    29041 aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga
    29101 atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct
    29161 gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac
    29221 tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac
    29281 aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa
    29341 atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac
    29401 tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga
    29461 tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc
    29521 cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca
    29581 gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa
    29641 tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga
    29701 aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa
    29761 tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga
    29821 gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga
    29881 caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa
    29941 acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga
    30001 atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg
    30061 aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt
    30121 gtaaaacagg atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca
    30181 tgtcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt
    30241 aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct
    30301 gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg
    30361 taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc
    30421 gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac
    30481 aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaa
//
LOCUS       DACEJR010000034        30463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEJR010000034 DACEJR010000000
VERSION     DACEJR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.034"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..552)
                     /locus_tag="IW651_001539"
     CDS             complement(<1..552)
                     /locus_tag="IW651_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0814265.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="IW651_001540"
     CDS             complement(678..2606)
                     /locus_tag="IW651_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0814266.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="IW651_001541"
     CDS             complement(2912..3628)
                     /locus_tag="IW651_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0814267.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IW651_001542"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IW651_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0814268.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IW651_001543"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IW651_001543"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0814269.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="IW651_001544"
     CDS             complement(7098..8558)
                     /locus_tag="IW651_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0814270.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="IW651_001545"
     CDS             complement(8548..9843)
                     /locus_tag="IW651_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0814271.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="IW651_001546"
     CDS             complement(10175..10852)
                     /locus_tag="IW651_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0814272.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11973)
                     /locus_tag="IW651_001547"
     CDS             complement(10882..11973)
                     /locus_tag="IW651_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0814273.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12074..12508)
                     /locus_tag="IW651_001548"
     CDS             complement(12074..12508)
                     /locus_tag="IW651_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0814274.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IW651_001549"
     CDS             complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IW651_001549"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0814275.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14546..16441)
                     /locus_tag="IW651_001550"
     CDS             complement(14546..16441)
                     /locus_tag="IW651_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0814276.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16499..18274)
                     /locus_tag="IW651_001551"
     CDS             complement(16499..18274)
                     /locus_tag="IW651_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0814277.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18324..19820)
                     /locus_tag="IW651_001552"
     CDS             complement(18324..19820)
                     /locus_tag="IW651_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0814278.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20014..21636)
                     /locus_tag="IW651_001553"
     CDS             complement(20014..21636)
                     /locus_tag="IW651_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0814279.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21964..23277)
                     /locus_tag="IW651_001554"
     CDS             complement(21964..23277)
                     /locus_tag="IW651_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0814280.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23522..23746)
                     /locus_tag="IW651_001555"
     CDS             complement(23522..23746)
                     /locus_tag="IW651_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814281.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23766..24134)
                     /locus_tag="IW651_001556"
     CDS             complement(23766..24134)
                     /locus_tag="IW651_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0814282.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24330..25040)
                     /locus_tag="IW651_001557"
     CDS             complement(24330..25040)
                     /locus_tag="IW651_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814283.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25058..25555)
                     /locus_tag="IW651_001558"
     CDS             complement(25058..25555)
                     /locus_tag="IW651_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0814284.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25592..26044)
                     /locus_tag="IW651_001559"
     CDS             complement(25592..26044)
                     /locus_tag="IW651_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0814285.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26179..26784)
                     /locus_tag="IW651_001560"
     CDS             complement(26179..26784)
                     /locus_tag="IW651_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0814286.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27234..27854)
                     /locus_tag="IW651_001561"
     CDS             complement(27234..27854)
                     /locus_tag="IW651_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0814287.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27856..28725)
                     /locus_tag="IW651_001562"
     CDS             complement(27856..28725)
                     /locus_tag="IW651_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0814288.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28748..29155)
                     /locus_tag="IW651_001563"
     CDS             complement(28748..29155)
                     /locus_tag="IW651_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0814289.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29174..30463)
                     /locus_tag="IW651_001564"
     CDS             complement(29174..30463)
                     /locus_tag="IW651_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0814290.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8630 a         6627 c         5021 g        10185 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    11581 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    11641 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    11701 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    11761 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    11821 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    11881 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    11941 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    12001 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    12061 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    12121 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    12181 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    12241 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    12301 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    12361 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    12421 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    12481 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    12541 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    12601 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    12661 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    12721 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    12781 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    12841 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    12901 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    12961 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    13021 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    13081 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    13141 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    13201 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    13261 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    13321 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    13381 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    13441 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    13501 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    13561 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    13621 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    13681 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    13741 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    13801 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    13861 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    13921 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    13981 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    14041 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    14101 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    14161 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    14221 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    14281 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    14341 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    14401 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    14461 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    14521 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    14581 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    14641 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    14701 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    14761 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    14821 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    14881 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    14941 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    15001 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    15061 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    15121 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    15181 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    15241 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    15301 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    15361 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    15421 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    15481 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    15541 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    15601 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    15661 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    15721 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    15781 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    15841 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    15901 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    15961 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    16021 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    16081 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    16141 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    16201 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    16261 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    16321 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    16381 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    16441 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    16501 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    16561 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    16621 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    16681 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    16741 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    16801 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    16861 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    16921 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    16981 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    17041 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    17101 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    17161 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    17221 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    17281 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    17341 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    17401 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    17461 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    17521 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    17581 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    17641 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    17701 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    17761 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    17821 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    17881 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    17941 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    18001 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    18061 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    18121 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    18181 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    18241 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    18301 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    18361 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    18421 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    18481 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    18541 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    18601 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    18661 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    18721 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    18781 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    18841 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    18901 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    18961 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    19021 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    19081 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    19141 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    19201 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    19261 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    19321 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    19381 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    19441 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    19501 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    19561 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    19621 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    19681 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    19741 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    19801 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    19861 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    19921 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    19981 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    20041 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    20101 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    20161 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    20221 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    20281 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    20341 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    20401 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    20461 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    20521 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    20581 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    20641 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    20701 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    20761 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    20821 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    20881 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    20941 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    21001 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    21061 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    21121 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    21181 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    21241 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    21301 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    21361 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    21421 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    21481 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    21541 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    21601 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    21661 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    21721 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    21781 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    21841 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    21901 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    21961 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    22021 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    22081 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    22141 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    22201 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    22261 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    22321 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    22381 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    22441 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    22501 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    22561 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    22621 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    22681 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    22741 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    22801 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    22861 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    22921 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    22981 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    23041 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    23101 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    23161 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    23221 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    23281 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    23341 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    23401 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    23461 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    23521 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    23581 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    23641 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    23701 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    23761 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    23821 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    23881 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    23941 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    24001 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    24061 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    24121 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    24181 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    24241 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    24301 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    24361 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    24421 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    24481 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    24541 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    24601 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    24661 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    24721 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    24781 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    24841 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    24901 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    24961 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    25021 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    25081 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    25141 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    25201 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    25261 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    25321 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    25381 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    25441 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    25501 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    25561 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    25621 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    25681 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    25741 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    25801 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    25861 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    25921 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    25981 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    26041 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    26101 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    26161 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    26221 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    26281 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    26341 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    26401 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    26461 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    26521 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    26581 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    26641 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    26701 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    26761 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    26821 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    26881 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    26941 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    27001 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    27061 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    27121 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    27181 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    27241 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    27301 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    27361 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    27421 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    27481 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    27541 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    27601 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    27661 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    27721 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    27781 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    27841 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    27901 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    27961 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    28021 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    28081 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    28141 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    28201 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    28261 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    28321 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    28381 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    28441 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    28501 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    28561 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    28621 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    28681 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    28741 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    28801 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    28861 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    28921 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    28981 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    29041 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    29101 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    29161 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    29221 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    29281 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    29341 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    29401 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    29461 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    29521 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    29581 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    29641 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    29701 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    29761 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    29821 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    29881 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    29941 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    30001 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    30061 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    30121 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    30181 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    30241 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    30301 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    30361 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    30421 cctgagtcaa aatattttcg atgataaaat tgataacgcc cat
//
LOCUS       DACEJR010000035        29921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEJR010000035 DACEJR010000000
VERSION     DACEJR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.035"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..402
                     /locus_tag="IW651_001565"
     CDS             25..402
                     /locus_tag="IW651_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814291.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1008..1622
                     /locus_tag="IW651_001566"
     CDS             1008..1622
                     /locus_tag="IW651_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0814292.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2395..4134
                     /gene="yfcC"
                     /locus_tag="IW651_001567"
     CDS             2395..4134
                     /gene="yfcC"
                     /locus_tag="IW651_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0814293.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4291..5475
                     /gene="iadA"
                     /locus_tag="IW651_001568"
     CDS             4291..5475
                     /gene="iadA"
                     /locus_tag="IW651_001568"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0814294.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5586..5789
                     /locus_tag="IW651_001569"
     CDS             5586..5789
                     /locus_tag="IW651_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0814295.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5890..6552)
                     /locus_tag="IW651_001570"
     CDS             complement(5890..6552)
                     /locus_tag="IW651_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814296.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6565..8067)
                     /locus_tag="IW651_001571"
     CDS             complement(6565..8067)
                     /locus_tag="IW651_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814297.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8615..10294
                     /locus_tag="IW651_001572"
     CDS             8615..10294
                     /locus_tag="IW651_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0814298.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            10421..10900
                     /locus_tag="IW651_001573"
     CDS             10421..10900
                     /locus_tag="IW651_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0814299.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            11119..11799
                     /locus_tag="IW651_001574"
     CDS             11119..11799
                     /locus_tag="IW651_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0814300.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11807..13138
                     /locus_tag="IW651_001575"
     CDS             11807..13138
                     /locus_tag="IW651_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0814301.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            13153..14115
                     /locus_tag="IW651_001576"
     CDS             13153..14115
                     /locus_tag="IW651_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0814302.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            14226..14564
                     /locus_tag="IW651_001577"
     CDS             14226..14564
                     /locus_tag="IW651_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0814303.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14643..15350
                     /locus_tag="IW651_001578"
     CDS             14643..15350
                     /locus_tag="IW651_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0814304.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15453..16934)
                     /locus_tag="IW651_001579"
     CDS             complement(15453..16934)
                     /locus_tag="IW651_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0814305.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            17243..17779
                     /locus_tag="IW651_001580"
     CDS             17243..17779
                     /locus_tag="IW651_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0814306.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            17857..19227
                     /locus_tag="IW651_001581"
     CDS             17857..19227
                     /locus_tag="IW651_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0814307.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            19294..19629
                     /locus_tag="IW651_001582"
     CDS             19294..19629
                     /locus_tag="IW651_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814308.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(19710..20567)
                     /locus_tag="IW651_001583"
     CDS             complement(19710..20567)
                     /locus_tag="IW651_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0814309.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            20674..21189
                     /gene="tadA"
                     /locus_tag="IW651_001584"
     CDS             20674..21189
                     /gene="tadA"
                     /locus_tag="IW651_001584"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0814310.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            21212..21296
                     /gene="ffs"
                     /locus_tag="IW651_001585"
     ncRNA           21212..21296
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW651_001585"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21506..22891
                     /locus_tag="IW651_001586"
     CDS             21506..22891
                     /locus_tag="IW651_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0814311.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            22928..23605
                     /locus_tag="IW651_001587"
     CDS             22928..23605
                     /locus_tag="IW651_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0814312.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            23613..24377
                     /locus_tag="IW651_001588"
     CDS             23613..24377
                     /locus_tag="IW651_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0814313.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            24379..25038
                     /locus_tag="IW651_001589"
     CDS             24379..25038
                     /locus_tag="IW651_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0814314.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(25068..25577)
                     /locus_tag="IW651_001590"
     CDS             complement(25068..25577)
                     /locus_tag="IW651_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0814315.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(25689..26687)
                     /locus_tag="IW651_001591"
     CDS             complement(25689..26687)
                     /locus_tag="IW651_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0814316.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            26888..27472
                     /locus_tag="IW651_001592"
     CDS             26888..27472
                     /locus_tag="IW651_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0814317.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(27549..28724)
                     /locus_tag="IW651_001593"
     CDS             complement(27549..28724)
                     /locus_tag="IW651_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0814318.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            28806..29186
                     /locus_tag="IW651_001594"
     CDS             28806..29186
                     /locus_tag="IW651_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0814319.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            29260..29622
                     /locus_tag="IW651_001595"
     CDS             29260..29622
                     /locus_tag="IW651_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814320.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9736 a         5005 c         6199 g         8981 t
ORIGIN      
        1 tataaaaaaa ggtgtgtgtt cactatggga aaattagtcg ctgttgcaca gtcgctgctt
       61 gatatactca agtggctgat ggaaggaagc aattggatat ttgtgttgcc actattgctt
      121 attagtctat ttatcatgtt tcgcatggtc agaaaaaaag aaacgtttct gatttttatc
      181 tggtatgcag catccgttgg ggttgctacc ttgatggttg aattggcgtt aggtagttgg
      241 gaagcttgcg tatttgcttt attttttgct gtactgtatc taatcattta tatcccatat
      301 atgaaaagaa agtctcggat aggcagaaaa ggtagacata caactacttt ttctagtaca
      361 gattctctcg aacaaagtag aagaacgaga agaagacggt aatatttagt aggtatacgc
      421 aaaaaaagat aaaataatgg ataaactatt gagatgagag atttatttaa ttggtggaaa
      481 aaagcttttt tcacttatat aaaagaatct tttttaaagg aatgcttgtt gtatcgggat
      541 aagccattcc tttttttatg ttatttatat atatataaat cataccaatg tttttttgta
      601 ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag aaacagctga
      661 ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga ccagtcacac
      721 acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc taatttattg
      781 tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt ctgcacgttg
      841 ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt gtgtttgaaa
      901 cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt ccttagatta
      961 ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg agtttatgtt
     1021 attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg ctgatgcttc
     1081 aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag atatatgcta
     1141 gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat cttgtaccaa
     1201 gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt gcaggacagg
     1261 caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt tcgaatgaag
     1321 gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt catccattaa
     1381 tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc agtggtttgc
     1441 gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat gatgcagtct
     1501 tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca acgatctttt
     1561 tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca agtggaaaat
     1621 agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa aaatggagcg
     1681 tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt tttgttttag
     1741 ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt tgttattttt
     1801 ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc agaaaaataa
     1861 aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt ctcttaatac
     1921 acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta catttgttat
     1981 cattattgca atgaagttta cctgatataa caaaattgtc caacaggata cctttttcta
     2041 atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa gtgtttatta
     2101 ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa caggacgaga
     2161 ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa taaaatactt
     2221 aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa
     2281 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
     2341 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
     2401 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
     2461 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
     2521 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
     2581 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
     2641 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
     2701 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
     2761 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
     2821 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
     2881 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
     2941 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
     3001 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
     3061 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
     3121 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
     3181 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
     3241 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     3301 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     3361 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     3421 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     3481 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     3541 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     3601 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     3661 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     3721 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     3781 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     3841 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     3901 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     3961 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     4021 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     4081 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     4141 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     4201 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     4261 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     4321 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     4381 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     4441 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     4501 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     4561 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     4621 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     4681 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     4741 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     4801 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     4861 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     4921 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     4981 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     5041 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     5101 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     5161 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     5221 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     5281 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     5341 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     5401 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     5461 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     5521 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     5581 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     5641 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     5701 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     5761 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     5821 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     5881 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     5941 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     6001 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     6061 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     6121 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     6181 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     6241 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     6301 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     6361 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     6421 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     6481 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     6541 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     6601 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     6661 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     6721 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     6781 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     6841 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     6901 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     6961 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     7021 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     7081 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     7141 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     7201 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     7261 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     7321 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     7381 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     7441 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     7501 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     7561 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     7621 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     7681 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     7741 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     7801 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     7861 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     7921 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     7981 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     8041 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     8101 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     8161 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     8221 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     8281 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     8341 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     8401 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     8461 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     8521 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     8581 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     8641 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     8701 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     8761 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     8821 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     8881 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     8941 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     9001 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     9061 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     9121 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     9181 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     9241 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     9301 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     9361 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     9421 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     9481 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     9541 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     9601 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     9661 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     9721 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     9781 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     9841 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     9901 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     9961 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
    10021 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
    10081 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
    10141 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
    10201 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
    10261 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
    10321 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
    10381 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
    10441 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
    10501 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
    10561 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
    10621 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
    10681 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
    10741 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
    10801 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
    10861 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
    10921 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
    10981 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
    11041 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
    11101 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
    11161 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
    11221 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
    11281 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
    11341 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
    11401 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
    11461 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
    11521 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
    11581 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
    11641 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
    11701 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
    11761 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
    11821 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
    11881 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
    11941 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
    12001 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
    12061 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
    12121 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
    12181 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
    12241 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    12301 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    12361 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    12421 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    12481 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    12541 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    12601 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    12661 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    12721 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    12781 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    12841 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    12901 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    12961 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    13021 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    13081 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    13141 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    13201 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    13261 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    13321 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    13381 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    13441 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    13501 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    13561 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    13621 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    13681 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    13741 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    13801 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    13861 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    13921 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    13981 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    14041 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    14101 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    14161 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    14221 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    14281 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    14341 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    14401 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    14461 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    14521 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    14581 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    14641 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    14701 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    14761 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    14821 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    14881 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    14941 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    15001 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    15061 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    15121 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    15181 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    15241 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    15301 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    15361 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    15421 atttggaata agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa
    15481 ccttttttca tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg
    15541 tgcgttgtat ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta
    15601 atgatcaaat cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac
    15661 tgctgcaaat ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg
    15721 atgagtaact gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat
    15781 tgactgattt ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat
    15841 ttcttttcct gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta
    15901 gaaaaactac tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa
    15961 taaaaataaa ttggtatttt attttctaaa tggttgagag gaaactgttg agttaagtaa
    16021 gtcatcaatt gaccgacttg tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca
    16081 atctcctgtt tttgtcgaaa agagatttga tttggaaaaa aacaatagaa aaaataatta
    16141 aagtaggctc tctctgtttc tgtctcttca caagggcgac tcactgaaaa agaagtaaag
    16201 tattctgaat aatcatgtgt atgctgcaat aagtccatca attcagtgat ttcagaagaa
    16261 gtttttggaa aatatgcctg ttgtttgttt cgtaatatga ataaagttaa cagcaagtag
    16321 atattttttt cttgttttga aattttttct atttgctcat ctgtactgtt catttttagt
    16381 gcatttaatt caagtgagga aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa
    16441 aagtaaagat agagacggat agataactca tttccttcaa gagtacagca attttttaca
    16501 gataggatcc gtaaatggaa tttttttaag gaatgattga gctgttttgt cagtttttcg
    16561 agataggagg gcgatatata aagttgttca agtaaatatt tttttggtac agcagtttgt
    16621 ggataattga ttagtaacat caaatagtta aaacgattgg attcttttag gtagcataat
    16681 tttaacttag aaaaaacagc taacttttta gtttgatcgg tttctttggt ttggatctct
    16741 cctttatgat tagaataaat aaattcatca atcaataaaa attgcttgag tgatacattt
    16801 atttttcgga tcagttcttt gattgtccgt tttggaatat gtaagtcatt ggataattct
    16861 tgtaaagaac aattattttt ttgaatgacc atttgtagaa caagtaactc tttttttctt
    16921 gcttcaatta gcataaatac ctccttttta ttctgttttt gtatatagtt agacaaaaac
    16981 tgtgagtgga aagtcatttc agtctgtgtt attctcacag agtgtcgcct tcttttttat
    17041 ataacgttat ttaatttttt tgttaccttg tttattctat tgtttatttc ctagggtatt
    17101 ttgtcaagat attcttgttt ttaaggcgga aaaattgatt tttaatggat ataactaaat
    17161 atgttttgat aattatcaat aatattttta gaattattta tgttatttat ttatgggtag
    17221 atgaaaagag acggaggtaa gaatgatggg gagagtaaca aattatagta aagaatattt
    17281 gctgttcaaa agtatggtat acgtggaaga atatggaatg aagtcaatta ctgcaagaga
    17341 gttagcagag ttctgcggtt gttctacata cccgatttat acacatttca aatcaatcag
    17401 cgggttaaaa gagaagatat tagaagaaat cactgtttat tttgaaaact acttagctga
    17461 atgcagttcc gatgatgttt taagcattgt ctacttattg aaagattttt tttctatcca
    17521 tgaaagtatt cgtgaaatca ttgcaaaatc tgatttagac atggattcgc tatttaagcg
    17581 gccatttttc tcatatgtca aaggtcacac gaagatcaaa gaagaaaagt cgcttgaatt
    17641 ggtatggctt aatgcattgg gaagtttgcg ctcagatgat tcttctcaag attttcttga
    17701 aatgatgaac gctgtttggg ataaaaacga ggtgacggat gaatacttgg aaaatatctt
    17761 aaaggaacag tcaccttagg cagcaacatg aaaaagtaga tcagccacct ttatttttcg
    17821 taaaaaagat tgtaaaatgt ctaacgaaac attggaatga aggaggaagc gacaacgtta
    17881 ccacagcaat ttatcaaaaa atatcgtctt cttttaggcg aagaagcatc cgattttttt
    17941 tctgcgcttg aacaaggaag cgtgaaaaaa ggatttcggt ggaatccact aaaaccggca
    18001 ggattggaca tggtccaaac ttaccatagc gaagaattac aacccgctcc ctacagcaat
    18061 gagggatttc ttggcactgt caatggaaag tctttcctcc atcaagctgg gtatgaatat
    18121 agccaagaac caagtgcaat gatcgttgga acagctgctg cagcaaaacc cggtgaaaaa
    18181 gttttggatt tgtgtgcggc tcccggtggt aaatcaaccc aattagctgc tcaaatgaag
    18241 gggaaagggt tattagtgac gaatgagatc tttccgaaac gtgcgaagat tttatcagaa
    18301 aatatagaac gatggggagt ttcgaacgca atagtgacaa atcatgcacc cgcagaactt
    18361 gtccctcatt tttcgggctt ttttgatcgg atcgtggttg atgctccttg ttcaggagaa
    18421 ggaatgttcc gaaaagatcc aaatgcaata aaagagtgga cagaagaatc tccgctatac
    18481 tgtcaaaaac gtcagcaaga gattttgtct tcagcaataa aaatgctaaa aaataaagga
    18541 caactgattt attcgacttg tacgtttgca ccagaagaaa atgaagagat cattagctgg
    18601 cttgtagaaa attatccagt aacgattgag gagattcctt tgactcaatc cgttagttca
    18661 ggacgttctg aatggggaag cgtagctggt ttggaaaaaa caatacgtat atggcctcat
    18721 aaagatcagg gtgaaggaca ttttgttgca aagctgactt ttcatggaca aaatcagatg
    18781 cataaagaaa agaagacacg aaaaaaaagt aaagtgcaaa tgacaaaaga gcaggaaaaa
    18841 ctctggacgg agttttcaaa tgattttcat tatgaagcta ctggaagact tttagtgttc
    18901 aacgaccatc tttgggaagt tcctgagtta gccccttctc tcgatggttt aaaagtcgta
    18961 cgtacaggtt tgcatttagg agatttcaaa aagaatcgat ttgaacctag ctatgcatta
    19021 gcgcttgcta caaaaaagat agagaatata ccttgcttgc cgatcactca aaaggaatgg
    19081 caatcttaca cagcaggaga aacatttcaa cgtgatggga atcaaggatg ggttttgcta
    19141 gtattagata agatacctgt cggatttggg aaacaagtaa aaggaacagt aaagaacttt
    19201 ttccctaaag gattgagatt tcactgagtg gattagttcg tttatttttt tgtctatgct
    19261 atgatggaga tagataaaaa ggagctgata cttatgaaaa catcaacaaa agtaacaatc
    19321 ggattaagcg tagcagccgc tgccagtgta gcgacagcag tcgttgtatc gggaaaagta
    19381 atggaaaaga tccatcatat gactactcgg acgaaagtaa aaaaattcgt ctatgacaaa
    19441 tttgatggaa atgaaaaatt actagaaatc gtagatggtc tttcagatag tgacttggat
    19501 tctttgatgg gaatgttagc aaaaatcaaa tcaggaaaaa agaaaatctc tgtttatggt
    19561 gattcaatca aagattcaac cgaagatgta aaagaccggc taatgcattt tgttgaaaaa
    19621 atgatgtaag agaaactagc ttttgaacac tattcattcg aaataagggg ttgtgacaaa
    19681 attattgtca caaccccttt aatctatctt taattatgtt catgaagcaa taaaagatag
    19741 caaaacagta aatcgttggc tttttttaga ttgaaaccag actgttcttg aaatttttcg
    19801 attcgatatt ttagagtatt acgatgaaga aacagtgttt ttgcagcaga gctgacattg
    19861 cctaattcat tccataattc ttgaatgatc acttccattt cattttgttt gatcagttct
    19921 atgtaacttt tgatcaactt actatctttt acagtatctt ttgtatagta atgtaaagcc
    19981 acatcttgca tagaaaaagt acggctgtga gtataaagat tgttttgctc tgttaaaaaa
    20041 atttttcttt cttcagcaaa caaagggacg aggtcggtgc caggtgaatg gaaagaacca
    20101 acgaatacag acgttgtcgt atcaaaatca gcatctaaag atagaaaaat accgttgatt
    20161 tcagtagcat caagataatc acggtttatt tgttcgacta agagcgcatc tgtgtctgta
    20221 taaaaaaaga aatcagcaga atagcgaaat aattccaaaa tagtatcttg ccattctttt
    20281 tttaagaatt ctccttttga ttcgacgtga agctggatca aacgaaagct tttttcttga
    20341 acacaagaag catctttaaa aagatagtga taccatgggt gttttttttg atcgttgatt
    20401 acagggaata acgctttcaa aaggctggtt tcttgttgag ataatgaatc ttgtttgatc
    20461 cagataaaag catcatcaat tgcaaaagaa aggatatccg cttctgatgc tggagagttt
    20521 tttttctgtg cttgtgggta aagagcaagt aatttatcca ttttcatctt gttcacctct
    20581 tgtatttatt ttaccataga aaaaaaagtt agttacagcc tttttatcat atgctaaagt
    20641 aaaacaagaa aaaacaaagg cagggaaggg taattggtaa atacaaatct tgatgcagta
    20701 gaaaaagaaa aatggatgcg ccttgcattg gcagaagcaa agaaagcaga aatgcttcat
    20761 gaagttccga ttggagcagt ggttgtattg gatggaaaag tgattggaag aggatataac
    20821 cttagagaaa ccacgcaaga tgctactaca catgcagaaa tgcttgcaat caaggaagcc
    20881 tgtgaaaaag tgggaagctg gcgcttggaa gatgctgcac tttttgttac cttagagcct
    20941 tgtcctatgt gcagcggcgc gatgattttg tctcgtgtag cagaagttta tttcggcgcg
    21001 tatgatccaa aaggcggaac agccggtacc ttgatgaatc ttttggagga cgagcggttt
    21061 aaccaccaag catatgtaga aggtggcatt ttagaagacg aatgtgggat gattttgact
    21121 gactttttcc gaaagctccg ccagcgaaaa aagaaattaa aaaaagactg gtcaacgaat
    21181 gaaaaataag gtatactatt ctttgccgaa aggctaggac aatggtgggc ttgtgaagcg
    21241 tgtcaggtct ggaaggaagc agcactaagc aggttccgcc atgtgtctga ttataatgaa
    21301 acgacaacag actttcgggt ctgttttttt tatttttata gtactgtaaa aatgaatgtt
    21361 cctgattttt tagccagact gatttatcga atattgtact tatacaattt gcttcaaggc
    21421 aggaaaaact ctgaaataga gcttgtttca atggaagaaa aagctgccta tggtatgata
    21481 aagataaaga gaggcgagag ggtacatgga aaaaatagtg attttacata cgaatgatct
    21541 gcattctcat ttagaaaatt ggccaaggat cagaaggttt ttggatcaac gtaaacgaga
    21601 aaacgaaaag aaagaaaaaa cgacaacgat tgcagtagat ctaggtgatt ttgtggatcg
    21661 ttggcatcca ttgtcagaag caacgaatgg acaggaaaat atcttgctga tgaatgaagt
    21721 tggttatgat gcagcgacga taggaaacaa tgaaggcgta ggaaattcca aagaagagtt
    21781 gaatcattta tacgatcatg ctgagtttga tatcatcctg gataacttat tcgataaaaa
    21841 tacgctccag ccaccggcat ggacgaaacc atataaaatc attacaacga aacaccagac
    21901 gaaaatcgga ctaattgcat ttactgctcc atttccgctt acttataatc cgaatggctg
    21961 ggacatacgg aatccttatg atattttgcc agaattagtc gaagatttac gttcacaagt
    22021 ggatgtttta gttttgatga gtcatctcgg gattcaggat gatttccaaa ttgcaaaaga
    22081 aataccagca attgatgtta ttttaggatc gcacacccat catttattca gagaaggtca
    22141 cgtaatcaat gatgtccaaa tagcagccgc tggaaagtac gggcagtatg tcggtgaagt
    22201 ccatttaacc atcgatgaag agaaaaaaat ccttaagcat tcagccaaag ccatccctac
    22261 cgaaacaatg acagctttta cagaagatga acaagaaata aatagttatc tagaacgtgg
    22321 gcatgaatta ttaaaagaaa aagaagtagc agatttgcca ttttctctga gtctagatat
    22381 cttcaatgag cattcgttta tccaagtagc ctttgaggcg gtgaaagaac gaggaaagac
    22441 ggaagcagca atattaaaca gtggtctgtt tttaactgat attccaaaag ggcgggtcaa
    22501 tcaggatcag cttcacactg cgttgccgca tccgatgcac ttgttaaatg tcacattggg
    22561 tggaaatgat ctgatccgct tagttttaga aatagaaaaa aatcgtaact ttttacgtaa
    22621 ctatcctatg aagggaatgg gatttcgcgg taagattttt gggcagatgg tctataatgg
    22681 aatcacatat gattctatta accatcaagt cttttggaag aacaagccaa tcgatctgaa
    22741 taaaaattat acttttacta ccgtagatca tttgatgttt gttccatttt ttccaacgat
    22801 tgagattgct ggaaaaaatg aatttttgtt ccctgaattt atccgaagtg tggtaggaga
    22861 ttacttgaaa gctcactacc caatcaaata aaaacaaggt ataataaaaa agataggtgg
    22921 tgaacgcatg tctgcaaaac aaaatgaaca gcagaacgtt gagcataaaa cagaaattgc
    22981 taagaaaaaa tcatcagctg aacaggaaac aacagtcacc gaagaactga cagctgcttt
    23041 ggaagaaatc gttgaggagc ctaaagcaga aaaagtcaaa ggcgagctag tgacactgtt
    23101 agatgacagt aattttttga taggtgaaag acattatcgg cttgtgtccg attatcgtga
    23161 aggatttaat gcagaaaagc ttggagaacg ttacagtgat gtactagccc gatatgatta
    23221 tatcgtagga gattggggtt atgaacaact acgcctaaaa ggatttttcc aagcggataa
    23281 tcgacgggcg catccagatc agcgaatcga ttccttggaa gactatcttt acgaatattg
    23341 caattttggt tgtgcgtatt ttgtcatcga gagaatcggc ggtaaaaaag aaaagacaac
    23401 tcagcgccga aaaaagaaaa aaaaccataa tcgtgcgtat atcgatgaga aaaaaggacc
    23461 agtcgcatct aatcgcaaaa aaccggttat taaaaatcga aaaacagagt cagcaaaaac
    23521 ctctgaaaaa gtaaaaaaac aagaacaacc taaacaagta gaaaaaacaa aaacagcatt
    23581 tacgatcaga aaaagggaag agtagtggat gaatggacta taaaggatat ttaattgatt
    23641 tagacggaac gatttatcgt gggacacaac ccataccggc cggtaaacga tttgtggaag
    23701 aattgcaaaa aagaaaatta cctttcttgt ttgttaccaa taacacgact aagtcaccag
    23761 aaacagtagc taatcgtcta gcagatgagt ttgacattca tgtagctcca gagacagtct
    23821 acacagctac tttggcaaca atcgatttta tgaaagcaga tggaaaaggg aaaaaagtct
    23881 atgttatcgg agaagcagga ttgattgact tgattttagc tgccggattt acatgggaag
    23941 aagaggctcc tgactatgta gtggtaggct tagataatta tctgacctat gaaaaagtag
    24001 ttaaagcaac acttgctatt caaaaaggtg ctactttcat tggaacgaac ccagataaga
    24061 atatacctac tgaacgcggc cttcttcctg gagcagggtc agtcatctct tttgtagaga
    24121 cagcaaccca aacgccgccg atctacatcg gaaaaccaga agccatcatt atggataaag
    24181 ccgttgaagt attagggctg caaaaagaag aagtaatcat ggttggagat aactatgaaa
    24241 cagatatcca agctgggata cgcaataata tagatacttt gctcgtttta tcaggtttta
    24301 caaaagaaga agatgtacct gggttgccta tcccagctac ctatgtgaaa caatcactgg
    24361 atgaatggag cttctaatat gaaaaattcg agatggcagt ggatggaata tgctggattg
    24421 ttcagcctat ttctgacact gatcagtctg gcagttggtg tgacgatcaa ctttcgacca
    24481 ctatacgtat ttgatatcgg acatttgcaa attttggatt atacttcttt agatcaagag
    24541 acgttactga aaaattttga tcacctaatg aattatctca ataatccctt caaaaccatc
    24601 ctcagcttgc cagatttccc agtttctgct agcggagccc atcactttta tgaagtgaaa
    24661 attttgtttt tagtggatta tgctgtgttt ttcatcactt tgatcccgag tattttgttt
    24721 atcaaatatt tgcaaaagaa tgatcggctt tggcggttga ttcgtccgtt tcaaatcggc
    24781 atgttactgc cagttgtttt cggttttttc atgatgatag gatttgatcg cttttttatc
    24841 ctttttcatg aaacgttttt caacaacgat gactggttat tcgatcccgt tactgatcca
    24901 atcatcaatg tacttccaga agaatttttc atgcatagct ttatcttatt ttttgtatta
    24961 ttagaattat tttttgctgt ttttcttttc ttaggaaaaa atagcttgaa acaaacaaaa
    25021 aagaaagaac tcgtctaaat agacgttttc tttctttttt tgttgaatta ttgagtaagg
    25081 cgacttcttt tttctggttc tttgtgtgct aatcgactgg cagcagcagc ggcgatcgct
    25141 ccgacgatat catctaagaa ggtatggaca tgaacgccat cgtggctgtt tagttctttt
    25201 aaaataccag gtttcacctt gtcgatataa ccataattcg taaagccgat cgtaccgtaa
    25261 acattcacga ttgacaaggc taggatctca tcgataccgt aaaggccttc gtcatcttcg
    25321 ataatatgtt gcaacggact catcaatttt ttcttttctg ctaatatatc taattggatt
    25381 ccagtgataa tggcgttatg tacttcacgc tttgttagca cgctgttgac actttcagta
    25441 caatccgcca aagtcaaatc agaaatatag tctttttgta aaaacatgac aagttgtgcg
    25501 atatcttcta cagttacgcc acgttccttc aatagttctc ttgccttttc ttctaaagtc
    25561 tcagttttga cgaccatatt attgctcccc ctttattttt tctttctcta tttcatgtta
    25621 acggaacatg aaaaaaatgc aagaaactgc tttcgggttt aacttgaaat gcaaagagat
    25681 ataaaaaatc aactaggacg aagattttta tcgtataagc ttgtgctgtg accaggctga
    25741 gttcgttcct ttggattaat atagtgtaaa gcgttgttca ctgcagtagg tgcttcaccg
    25801 agcccagtag caatcagttt cacttttcct tcataggtac agatgtcacc agctgcataa
    25861 acgcctggaa tagaagtaga catatctgat tttacagtaa tggcatttct ggtactatca
    25921 agtccccaag aagaaagatg ttctaaatta gaagagaatc cataattgac gatcagagaa
    25981 tcaatcatca aatcaatcgt ctcgtcactt ttcacttttt ttaaccggat atctgtcagt
    26041 tccccattat tgcctgataa tccatcgatg agatagggag taagcaagtt gacagatgaa
    26101 gatttcaagc gtgatacact atgttcgtgc gcacgaaatt ccgggcgacg atgaatcaag
    26161 taaacttcgc ttgcaatagg ctctaacatc aacgtccaat cgatagcaga atcaccacct
    26221 ccagcaatag cgactttttt tccagcatac ttcatcaaat cattcacgaa ataatccaat
    26281 ccgtggtttt caaatgattc agcattatct aaattcaatt ttcttggctg gaatgaacca
    26341 tttcctaaag ctaaaatgac tgctttggaa taatggatgc ctttatttgt agtaatctcg
    26401 ataacttcgt cttcacgagt aagcgagaga acttcttctt ttaaatggaa tgtatggtta
    26461 aaagtagtta attgtttttc taattgatcg attaattcac ttgcttttat tgctggatac
    26521 ccaggaatat cgtaaatata tttttcggga tacaaagttg ctaattgacc accaagttga
    26581 ggtagactgt caatgatttt tgttttagct tggcgcattc ctgcgtaaaa agcggcaaac
    26641 atccctacag gacctgcacc gatgattgta atgtcataga tttccataaa aattcctccc
    26701 ttttcacact atagcagaat ttaattgaga atgcttctca aaaatgtttg aattcgaaat
    26761 cgaaacggta agttatagaa tgtatctagt gatgaaaaat aaaagaaaac aaatctagta
    26821 tttttacgag aggttaggta ttatgttatt attattatga agtatgcaaa tattaggagg
    26881 aaacaatatg gcattcccac aactagattt aaaaagtgaa aaaggaccaa aagctgttat
    26941 caaaacaaac cgtggtgaca ttactgttca attattccct gaactagcac cgaagacggt
    27001 tcaaaatttc attgaacttt ccaaaaaagg ttattatgat ggcgtgattt ttcaccgcgt
    27061 tattcctgat ttcatgatcc aaggcggcga tccaactgga acaggaatgg gtggcgaaag
    27121 tatttatggc gaaagttttg aagatgaatt cagtcgtgaa ctgttcaacc ttcgcggagc
    27181 tttatctatg gcaaattcag gtccaaacac aaacggaagc caattcttta ttgtaaataa
    27241 cacaaatgtg ccagctaata tgcttggaca actagaaggt gctggattcc catcagaaat
    27301 cattgaagcg tataaaggtg gcggaactcc ttggctagat ttccgacata cagtctttgg
    27361 acatgtacta gaagggatgg acacagtaga tgaaatcgca aatgtacaac gtggtccaca
    27421 agaccgacct gtacatgatg tagtgattga aacaattgaa atttcagaat agtttggtat
    27481 ggtacggagt aattatgaaa aaggacgaaa cctagtaagg tctcgtcctt ttattttgaa
    27541 taaatgattt aatcgattgt ctctaaagca tacttgattc gattaagtcc ttccagtaaa
    27601 gtttcttctg gacaagcaat attcaagcgc atatgcccat gaccggaagg accaaacgtg
    27661 atgccaggat ttaatacgac ttttcctttc cgaacaagag tgttctccaa ttcttggtcg
    27721 tctgaaatat aggcggtaaa atctaaccac attaagtaag ttccttcagg gttcattatt
    27781 ctcactttcg gaagatactg ttcaaaaaac tgattaactg tttgaacatt tttttctaga
    27841 taaggaatca gttggcttaa ccattctcca cccgtctcat aagccgcttg ggttccaatc
    27901 agtccaaaag tgttgatttc gtgctgttgg ttcatcacta aatgtttttc aaaatcagct
    27961 ttcatctctg gatttttgat aaataccatt gaatttttga ttgcagctag attgaaagtc
    28021 ttggttgcgg aagtaaaagc aactagtaga tcatctaaat taggatcaat cgttagcatg
    28081 gaagtaaatg tatgatcgaa taaagttaaa tcttgatgga tctcgtcact aaacagaatc
    28141 acttgatgtt ttaaacacaa ttcgcttaac tgtttcaatt cttctttgct ccaaacacgc
    28201 cctccaggat tgtgaggatt gcataaaatc attgctttta cattgttttc ttcgattttt
    28261 ttctccatgt cagacaagtc catgatgaaa tgattatttt tttctagcaa agagcttcga
    28321 acgatgtgac gttgatttgt ttctataata gaggaaaatg gaggataaac tgggtcatga
    28381 atcaaaatag aatcgcctgg ttttgtaaaa gtttgtactg ctgcagctaa actggttaac
    28441 acaccacttg taaaaacgat attttctttc gtcaactcca cagcatggtg ttggcgttcc
    28501 caatgaatga tagcctgata tagtttatca gacagagtgg cataaccaaa gattccatgt
    28561 ttgatgtatt cactaaacgc tttagctacc ccaccgggag acaagaagtc catatctgct
    28621 acccacaaag gaagtaagtc atccatttga tatgtctcag caattgcgtc ccattttaca
    28681 ctttctgtat cttttcttga aatctgttta tcaaattcga tcatcttaag acctccagtc
    28741 attttcgtcg cttctttttt tgagctattt acagtataat gataaatgcg aagagaggga
    28801 agtagatgac ttataaaatt ggacaaataa ttgaaggaaa agtgacaggg atacagcctt
    28861 atggcgcatt cgtttctctt gataacgaga cgcaaggact tattcatgtt tcagaagtac
    28921 aatccggtta tacgaaaaat atccattcat tgttaaaagt cggacagcca gtaacagttc
    28981 agatcatcga cattgatgaa tattccaaaa aaatcagttt atctttaaga acgttagaaa
    29041 aaacaaatcc agctgttcca tatcgcagaa aaaggtattt cacgaataaa aatcgtaaga
    29101 tcggatttac cactattgcg gagcagttac ctatttggat agaagaagca ttatctgaac
    29161 tagaacggaa agaaaataag aaataaaaag tgtatttttt ctgaaaatgt gctagaattt
    29221 ttatgtaagc catagaacaa gttgatcgag gtggaaattt tggcaagcaa aagagttgct
    29281 ggtacaatta tgttgcattt ggaggatggt actaaaaaat ttttggtcca ttcaattgat
    29341 gacaagttgg aatttgcgtt agcggaactt tctgaaggaa agactggttt agcgaatata
    29401 cttaatttct taaaagaaat agttcatatc gatgttagca agattagttt gatggaattg
    29461 acaaatacac atattaaaga ggaaaatgtt ccgttgtttg tctttgaaac tgaacaaaag
    29521 aatcaaagag aagaattagg cgaaggatat atttgggaag aaccaggtca aatgcgctct
    29581 gttcttggaa cttacgaagt agagggagtg cctttcttct aaaatacaga ttaagcgaac
    29641 aaagaagttt gaattttttt caaacttctt tttttattgt ttggtccaaa aaatacaatg
    29701 aaatgttctg ctaaagatag ttttttagtg cattttattt tcaaaatatg ttgaaaacgg
    29761 ttgtataacg aataataata attgatcgaa aaatcatttc acaaagtaat gcttaattta
    29821 tagttgacca aggaagaaat acatgataaa tttaacagcg ttgattctga agtggatgtt
    29881 atatcacatt tctaaaaaat ttaaaaaata gttattgaca t
//
LOCUS       DACEJR010000036        29189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEJR010000036 DACEJR010000000
VERSION     DACEJR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.036"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(225..560)
                     /locus_tag="IW651_001596"
     CDS             complement(225..560)
                     /locus_tag="IW651_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814321.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYEGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(734..1228)
                     /locus_tag="IW651_001597"
     CDS             complement(734..1228)
                     /locus_tag="IW651_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814322.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQYFVWMRHEHCWCNPFEL
                     KKENEIRTKIVNGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            complement(1225..1452)
                     /locus_tag="IW651_001598"
     CDS             complement(1225..1452)
                     /locus_tag="IW651_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814323.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(1618..3588)
                     /locus_tag="IW651_001599"
     CDS             complement(1618..3588)
                     /locus_tag="IW651_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814324.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(3603..4469)
                     /locus_tag="IW651_001600"
     CDS             complement(3603..4469)
                     /locus_tag="IW651_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814325.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(4459..4692)
                     /locus_tag="IW651_001601"
     CDS             complement(4459..4692)
                     /locus_tag="IW651_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0814326.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(4704..6866)
                     /locus_tag="IW651_001602"
     CDS             complement(4704..6866)
                     /locus_tag="IW651_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814327.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYQKEEKEAKAKK
                     AKVKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEEVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLLIPDDPNMFESEVEEATDFYSAVEQLEVATN
                     GLERLAETFNSSEEVEFEALAEDIPDEKEALFADDDKPTEAENQEVSIEETQTQIQTE
                     TYQQSKIDEKPESQSDKENYVYEVEKIVSESDVPKSLDADFLEKNFEQIIADKFQPVV
                     LDEINDPVDLNEEMLSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGK
                     QMLADELLRIEQATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIENSLTDVQEKTLEIVEKTKSENEAEFHDTKEKITDVVVK
                     YFANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYRSNSEKLAEQS
                     EALREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVTAMACGTIL
                     GSSYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVIS
                     IDGKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(6866..7108)
                     /locus_tag="IW651_001603"
     CDS             complement(6866..7108)
                     /locus_tag="IW651_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814328.1"
                     /translation="MVFDREKMLAHANEVLMSGLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTIDRKKGVLQ"
     gene            complement(7218..7556)
                     /locus_tag="IW651_001604"
     CDS             complement(7218..7556)
                     /locus_tag="IW651_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814329.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            complement(7955..11596)
                     /locus_tag="IW651_001605"
     CDS             complement(7955..11596)
                     /locus_tag="IW651_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814330.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLIDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDENGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGNGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLSKETDKEQSDVKKKFIPTPNINQTDKYETNNGLGMSKDNQSIREP
                     ELTNDNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKSP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(11599..13548)
                     /locus_tag="IW651_001606"
     CDS             complement(11599..13548)
                     /locus_tag="IW651_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0814331.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPKKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(13590..14648)
                     /locus_tag="IW651_001607"
     CDS             complement(13590..14648)
                     /locus_tag="IW651_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ0814332.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(14767..15222)
                     /locus_tag="IW651_001608"
     CDS             complement(14767..15222)
                     /locus_tag="IW651_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814333.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(15806..16609)
                     /locus_tag="IW651_001609"
     CDS             complement(15806..16609)
                     /locus_tag="IW651_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0814334.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            17611..18834
                     /locus_tag="IW651_001610"
     CDS             17611..18834
                     /locus_tag="IW651_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0814335.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNELVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            18869..19636
                     /locus_tag="IW651_001611"
     CDS             18869..19636
                     /locus_tag="IW651_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0814336.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            19826..20224
                     /locus_tag="IW651_001612"
     CDS             19826..20224
                     /locus_tag="IW651_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0814337.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(20530..20925)
                     /locus_tag="IW651_001613"
     CDS             complement(20530..20925)
                     /locus_tag="IW651_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814338.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(20926..21303)
                     /locus_tag="IW651_001614"
     CDS             complement(20926..21303)
                     /locus_tag="IW651_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814339.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKQAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(21293..21688)
                     /locus_tag="IW651_001615"
     CDS             complement(21293..21688)
                     /locus_tag="IW651_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0814340.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKTSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELFEK"
     gene            complement(21755..21940)
                     /locus_tag="IW651_001616"
     CDS             complement(21755..21940)
                     /locus_tag="IW651_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ0814341.1"
                     /translation="MPKTSREIIKILKSNGFKKTGQTGSHAKFYNSKTKKTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(22022..22276)
                     /locus_tag="IW651_001617"
     CDS             complement(22022..22276)
                     /locus_tag="IW651_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814342.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(22298..22543)
                     /locus_tag="IW651_001618"
     CDS             complement(22298..22543)
                     /locus_tag="IW651_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814343.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAELDAIGNIHFS"
     gene            complement(22550..22834)
                     /locus_tag="IW651_001619"
     CDS             complement(22550..22834)
                     /locus_tag="IW651_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814344.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(22937..23119)
                     /locus_tag="IW651_001620"
     CDS             complement(22937..23119)
                     /locus_tag="IW651_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ0814345.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(23325..23864)
                     /locus_tag="IW651_001621"
     CDS             complement(23325..23864)
                     /locus_tag="IW651_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814346.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(23977..24243)
                     /locus_tag="IW651_001622"
     CDS             complement(23977..24243)
                     /locus_tag="IW651_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0814347.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(24245..24466)
                     /locus_tag="IW651_001623"
     CDS             complement(24245..24466)
                     /locus_tag="IW651_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ0814348.1"
                     /translation="MPVISIRVSEEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            24692..25510
                     /locus_tag="IW651_001624"
     CDS             24692..25510
                     /locus_tag="IW651_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0814349.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            25514..25810
                     /locus_tag="IW651_001625"
     CDS             25514..25810
                     /locus_tag="IW651_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814350.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(25992..26345)
                     /locus_tag="IW651_001626"
     CDS             complement(25992..26345)
                     /locus_tag="IW651_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814351.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(26365..27420)
                     /locus_tag="IW651_001627"
     CDS             complement(26365..27420)
                     /locus_tag="IW651_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ0814352.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            27636..28034
                     /locus_tag="IW651_001628"
     CDS             27636..28034
                     /locus_tag="IW651_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814353.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGNYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT         9038 a         5273 c         4397 g        10481 t
ORIGIN      
        1 ttatattctc acaatagcat ttaataataa atatactgtc taatatcata ttggtactta
       61 tatactgtcg tttgatttat atatttccct cttctttctt tagaatctct ctttattttg
      121 atataataaa atagaaatcg caccaaacaa tgaaatggcg gaaaacattt cattgaaaaa
      181 aggattgaac ataatgttca atcctttttt atagttataa tcaattaaca ttcggtgtgt
      241 gaaattgaat cttcaatgct ttcacatgga aaattattga ttccttcctc actcatttcc
      301 catgtagtat agaagggagt taactgtcct tcatactcct gaataataaa atattttcca
      361 tcattgatat ggttacaaca gtacactcca atagttggat actcgactac taaaaattgg
      421 tctggatcaa actgttcttt ttttaacatt tctcgtattt gaaacaaatg atccttctct
      481 aagcaatcag gtaattcctt tattctttgc gttactaatg acccctctat cgctttccat
      541 tcatttaaat attgtttcat aacaattcat tccttttatg ttaactattt ttcctaggaa
      601 attgttttaa aacgccactc tcccagaaaa cgtatgattt ggtttatgga atataccttc
      661 cagctaaaca caattttcca ttactatatt gtaagcttgc tttcacttca taaaaattgc
      721 tgatatactc ttttcaaatc gaactcttcc tatctgagaa gggtatttct tgcgctagcc
      781 cctcataagt tccatcttca ttttcccaaa tttctacctg aaacactgaa ttattggcta
      841 atttttgttg caagagataa tagggttctt cttcatactt ttgatcgact tctttaagac
      901 attcaatagc atcaatatca atattttgaa agcccaattt ttctaggtaa caatcgtacc
      961 aagtacccca tgtattatcc agaaatgaga caaatgcatg atccatatca tttgctataa
     1021 aagattcatc ttcccatctg aagaggtaat cccactcacc atttacaatt ttagttctga
     1081 tttcattttc tttttttaac tcaaaaggat tacaccaaca atgctcatgt cgcatccaaa
     1141 caaaatattg tccattctca tctattttaa attggctgag atcacctttc aatctaaata
     1201 cacggttctt ttctggtaac ttattcatgc ttcaaccacc tcatactgtt ctctcatacc
     1261 tttgacttca ttaaaaaaaa ctctcgcttt ttcttcatca gtaaagtttt tatacaactg
     1321 attacaataa tgaggttttt gaagtttctg aatacctata ctaacttcaa atccttcttc
     1381 atgttgaatt aggattactt ctgtgtctct acctagaaat attcgatcta aaatttttat
     1441 gtttggtttc attatttaac ctccactata attatttcac tcagtaaggt ttcgctttat
     1501 gaaatttcta tagttctctt ttcacaaaac gtaccatgtt ctctaagact gtttttagtc
     1561 tcaaatatac atgtttttct tatcctttcc ttatacactc aaaatcaaag ataaaactta
     1621 aagaataaac tctccaataa caaaattatt agtcagatcc actggtctcc tgatctgaaa
     1681 tttattactt cttcctgttg tcaaatactg tctcaaatca acagaaagtt tttgatcatc
     1741 ttctcctttt ctagcaggag caactttttt ttcatacttc attaactctc cactggtcat
     1801 ctttccaatg atagtgtaat taaaatcact ttcaaaactt tggtaaattt ttccatctgt
     1861 attgaatata tcagctgttt caatatcttc tttgtattca ttgtcttctc ctgaaccgat
     1921 tgtatcaaat agaaaggcaa gacgaattcc atcttcttca agcattgtta atcttttttt
     1981 aattagccca acggtttcaa tagataactt atcgaagccg tgaatcataa caatatcatc
     2041 tttttttgct tgtgatgtga tgaaatcaaa ggtatttaaa aattgagctt ccataacatt
     2101 ggtttctgct cttagatcac ctaagttata gtaaaactgt aatttgtcac ttttaccgat
     2161 ttctttcagt gtcgttcttt gattaaacat cctagagaag ctgtctaatg ctgttctcaa
     2221 tgtgtctttt aatacaataa tacgatcgac ttctttttca gtaccagatt ttttggcttt
     2281 tgtttcagca ttagtcaaat agctaataaa atccccaaga actggcattg tatcactact
     2341 gtttaccaaa agttttgaac gttcttcata attaatatcc caaagtttct gacttatata
     2401 aaagttacct aattcttttt tcaataaaac tctagttgca gaatctaatg ttctgcctgc
     2461 taacaaataa aaaatctgag ttagtttatc gagattccgt ctatataaat caccaatgtc
     2521 cttttgatta ccaaaaagct ccaaagagtt aagaccttct tcagccaaat taaccttttc
     2581 tattacactg tttaacgaag gtttggcaac aaatgttcta ccagttggat caccaaagta
     2641 atcaaattta tttaagacaa tatgaaaagt tcgtttatga tttgccattg cattattagc
     2701 aatcaattgc ccccaaagtg atgatgctga gattttttca tcatagcctt taattgtagc
     2761 gtttttgtgt gatgccacta aaactttact tgtaaaagaa tcatccagtg ccataaaagc
     2821 tacaccactt gcgtagctag tagacatagt gccgatagcc catccattcg catcatctaa
     2881 acctttgcga attgcatgat catttcctgc aaaatccgaa ctcatagacg tatactcata
     2941 gacattcccg attggttcac ttaaaagtct atcaaacaga tcacgttgat ctcctccata
     3001 tgacataagc tttaatccat acattttttc atcatacaac cgttggagtt taattagctg
     3061 ttcgctgatt ttattaggag aatcggatac taataggatt tctagcttcc aatcaataat
     3121 tttatttgcc atgctgtcta gtaaagatgc tgtattcaaa tccataatcc gcataacagt
     3181 tttgctaatt tcagcatctg aattttttcc ttctaaatta ttttcataac tattcgttga
     3241 tcccccaaca ttctcgtgca ttatttcttg ttgatctttt tgtgacactg gcttatctgc
     3301 ttcaattaga taggctttta ccccttctac tctcacttca ggaatagctt taaccatcca
     3361 accaaatcca taatttctat tcattccata agtattcact aatttcacaa ttgttcccca
     3421 ccgcttacct gtcctaatgt aagatggtgt aaattgataa ccaactaaag atgttaaatc
     3481 actaacaaag gtattattca ctacctttga atttctagtt ttttgttctt ttttagtttt
     3541 tctctttttt ttgaaattta atagttgtga tctacccata atttccattt aatctctcac
     3601 ctctatttca gtgcataaat atcagataat atttcttttg tttgtttagc tgacaactga
     3661 gtgacactat aataaagtcc tctactcgta tagttttcta aacgttccac accttcatcc
     3721 aataccatct tagattgaga tttcaagata agatattgga ttactgttgt tttcacacca
     3781 tcaatatatt ctgatacaaa tcgttcttgc tgatctatta tgtcaaagac tgctttgtta
     3841 ctccttgcat tccgtttttg tattttcata tttcgagttt gtctttccgt attctgaggt
     3901 tgagatgaag tgattttgat ttcagctaag gaacgatctc tggtattttg atattgtatt
     3961 gatttcgatt cctgagccat aacttcttct ggaaaagcgg ttagtgatgt agccccatct
     4021 agattgaaca ttaatgctac actcccatct cgatatacga ttgttccatc ttgtttcact
     4081 tttaaaatct ctgtgcctcg aaatggtttc tttttcccca tagatactct tgctgaatta
     4141 actactgatt tgaacagtct aatagaacca gaaatcaatg aaccactaga tggatcaata
     4201 aacatatact ggctaattag aataagaaat ccaattatca ttaataacgt tggaacaaaa
     4261 ataaatttta gtttaatcgt atcagaaact aagtaaacaa ttgatccaag aacaacgact
     4321 gcaacaacta tagctgatac aattaatctt tttgctgtaa cagaataggt gctattgaca
     4381 tgcttttcaa gccattcaac tctgacaatt gtttcagaag gtttttgatg taagttatgt
     4441 ttaaatccat agtttgagtt atctgccatc agaaatactt cctttcacgt tcaacttacg
     4501 gtaaacacct acagttatca ctattgcaac aataagcaag ccaattaaag atattgactt
     4561 gctcattgtc tcgttcgttt gtggtagttc atttacctga tacacgagct gtgcttcctg
     4621 taagtgctga gaagaggtca tgttttcttg attgttttga attactggat aaactatttt
     4681 ttcttctcgc ataatagccc tccttagtta ttatcagata agtgagcttg aattttgtac
     4741 tgatttccgt ctggagtttt cacaactgct ttttctccat caatagaaat cacttcagcc
     4801 ttagttgttt tcccatcaat actgatgaca acatctttcc caactttgaa ctctgtagtt
     4861 ggtgttgctt catagctact atttgttgaa gcagagttcg tatttgtccc aatcgaattt
     4921 tccactctcg aaatagatgt ttgagtttga gcctgtgctt gttgattatt atgactaata
     4981 aagtaggacg atcccaaaat cgttccgcaa gccattgcag taacagcaat gatagtagcc
     5041 atctttttac ttgtaaaatc acgttttgaa cttgattgat tttctgtagc aactgctaat
     5101 gatttcgttt tttctgccat ttgagctaga tcacttcggg aacgagataa gtcttctctt
     5161 aaagcttcac tttgttctgc caacttttcg ctatttgatc tataaccctc tgctaaatct
     5221 ttgacctctt tcattcgatc tttatattct tcgatagttt tttgttgctc tacaagtttt
     5281 gtttctaatt ctttcgtgtt gttagctact tgcttttcaa cttcttcttt gttcgcaaaa
     5341 tatttgacaa ccacatctgt tattttctct tttgtatcat ggaactcagc ttcattttct
     5401 gactttgttt tttcaacaat ctctaacgtt ttttcttgca catctgttaa tgagttttca
     5461 atttctggtt tcaatgaatt gataatccgt tccaccacaa cttcttttgc ttcttggttt
     5521 tgtttcaatg catcgttttt aatagattca attgcttctt ggtactgttg atcaagactt
     5581 tcttcagttg cttttctata ttcatcttct tgttgtttaa cttctggtaa atgttcagca
     5641 tcataccgtt gctctagttt ctttagttca gcatccaaat aagcctgttt cgccatatca
     5701 taatctttac gaatgatact ggatttttcc atcactaaat catcaatctt agatgcttct
     5761 gcttcgtagc tctctttagc aaccgataat gtatttgtaa actgattttg accatttaat
     5821 gaggttgctt gctctatccg caaaagctca tcggctaaca tttgctttcc tttgaggatc
     5881 aattgctcct tcgtcttatt taattcactg ttgattttat tattagcgat agaaatacga
     5941 gcattcatac tctctttagc taaaaacagc aatggtttca actcagctat ggatagcatc
     6001 tcctcgttta aatcaacagg atcattaatc tcgtctaaaa ctacaggttg aaatttatca
     6061 gcgatgatct gttcaaaatt tttttctaaa aaatcagcat ctaagctttt cggtacatca
     6121 ctttcggata cgattttttc tacttcataa acataatttt ctttatcact ctgggattcg
     6181 ggtttttcat caatttttga ttgttggtag gtttctgttt gtatttgagt ttgtgtttct
     6241 tctatgctta cttcttgatt ttctgcttcg gttggtttgt catcatctgc aaaaagagct
     6301 tctttttcat ctggtatgtc ttctgctaag gcttcaaatt caacttcttc agaagagttg
     6361 aaggtctcag caagtcgttc taatccattt gtagctacct ctaattgctc cactgcgcta
     6421 tagaagtctg tagcttcttc aacttctgat tcaaacatat taggatcatc cggaataagt
     6481 aataattgac cactactatc cataaaacgg acacctgcta atagcttctc tttaaatttt
     6541 tctggtagat cagctacata attcaaactt gacttcaata gaacgacata cttttcggaa
     6601 tcgagaatag catcatcctc atctataact tcctcactgt tgagcttttg tacatcttca
     6661 attaccatat aatttttctt tggtaaatct ttggtcagca acgcatcgaa ttctttccat
     6721 acctttacct tagctttttt tgccttcgct tctttttctt ctttttggta cacttcgagt
     6781 aattgtggct tcaaagagag tgcaactaca ggaactgttt ctgccacata attaatagat
     6841 tcggtaatat ctttgaattt tgccattatt gtaaaactcc tttttttcgg tcaatagttc
     6901 ttttctgttt gtctagcata tatacatatg ctttttcgaa tttaataagc gtttcaagtc
     6961 tggctttttc aatcttttta ctcccattcc gataatcata gaactggtta acattcatat
     7021 tcattatctt tgccagttcg gtacctttca gaccgctcat taatacttcg ttggcgtgag
     7081 ctaacatttt ttctctatca aaaaccatct tacatttact ttcttccctc acttctaaac
     7141 tacgcttgga gcgtagtttt gtggtaaaaa gtaaattgaa caaatcggca tattatccaa
     7201 atttgttcaa ttcactttta acctaactgt tcattttttg gaaattttga aactagatag
     7261 ataccaacaa ctggaataag tgctgatata atattagctt tttggttcaa atcattacct
     7321 gttttagtac ccattgtgta aagcataacg gttgtaatta cagggataat aaagattgca
     7381 ataatcgccc caacatattt taatccatct ccccattttg catcaccaaa aaatttcatt
     7441 gcagtgaatc ctggtttcaa cgaaaaaacg aatgccaaga aatacaaaac aacttgtact
     7501 aaagttttca tatcaggcgt cattccgcta accaaatttt taatagaatc gaacatataa
     7561 aaacctcctt tttaatattt ttaataagtt aattatatca tcgacaaatc ttatttgttc
     7621 acttgtcaat gatctaacta agaaaaagac gtctcgatat ttacatcttt atgttatcat
     7681 aataacataa atttttgatg attaaaagag attttccatt aaattatttc acttttttta
     7741 ttggctaaaa atgctggttt atcaagtttt tgtttagata ataattctgt tctttaccag
     7801 cataagccct ttttaattaa acaactttga acagtaaata tttaattcat ttatagataa
     7861 ttttaaaaat ttttttgcat aaatagaaat ttttcttgac atcttcagtt aaaaataaaa
     7921 gtaaaaagaa ggagcaaatc aatgctcctt ctttttattc gtcttcagac ttattttcag
     7981 ttgatttctg attaccgtat tttacaatat agtccttgac tgacatggta gatttcttca
     8041 ctactttacc agtcttggat gctcctgtag cagtagcctt ggcaacctta gccgttcctt
     8101 tacctatttt aatagcttcg tttttagctt cataggttat ccctgctaga ctaccttttg
     8161 gagattttgc taaagcttta tctgctgagg tgtaaacatt tttaccagtt ttcgatagat
     8221 attcaacggc tgatttggct gttgatggtt tcttggttga ttgattagat ttccctgaat
     8281 tatctgaagt agatttagaa ttatttgttc tattattatc atttgtcagt tcaggttctc
     8341 taattgattg attgtctttc gacattccta atccattatt tgtttcatat ttatctgttt
     8401 gattaatatt aggagtagga atgaactttt tcttcacatc tgattgttct ttatccgttt
     8461 ctttcgatag cgattctctg tctttaagtg ctgtgtcatt ccgaatagca ctattatcaa
     8521 agttactgtt aagctctctt gactgacttt caccagatgc tccatttcct aattcccgat
     8581 gcactctgtc tgacacatcc tcaccactca tttcatttct tagttcttga tgagtttgat
     8641 ctaatgtaat ctcgtcatct tcattctcag gaattgcaac agcaattggc gtatgccgtt
     8701 gttgttcgag ttctctattt cgatctgcca caagcgcatc tctcgtctca atgatattcc
     8761 cattctcatc gactaaatcg ccattcacat tatatccatc gctgtgttta ccaagtttat
     8821 taccaaactc atcaacaaga tttccatctt catcaactaa acgccctttt tcgtcaacta
     8881 aacgtccgtt actatcctga ccaaaatcac catttagcaa cctgcgtcca ttttcgtcaa
     8941 caagatttcc atcttcatca actaagcgac ctttttcatc aattaaacgt ccttcgctat
     9001 ccataccaat attaccatct tcactatctt ccaatggttt accattagta tctataaggt
     9061 ttccatcttc atcgatcaaa cgcccttttt cgtcaataaa gttgccttta gcatctttac
     9121 cgtatttact atcttttaat atttcaccat tttcatcgac aaagtttccg ttttcatcaa
     9181 ccatacgtcc taaatgatca atatattgtt gggagttatt acgtagttga tcttgacgaa
     9241 ctaaatcttt gaaagaggta ttattgattg ttgaaccaac tccgccaccg ttgaatgaca
     9301 ttccttctgg tgtttcagga attactttat cagcaatgga cttagcatca gcattgctat
     9361 ctcccatatt gttcaaacgt tcagccattt tttgcgctcc aactgctcca agacctcctg
     9421 caaccatatc ttttactgag ctgttgccac catcataata gggattttta gctccttggc
     9481 ttttcttgat tgctttagaa acgccgtcca caactcgcac agcatcattt ctattaccaa
     9541 ataataactt agcacgttca tcaaacccaa acgctacttc aagtggtaag ccaataagca
     9601 tattaatcaa attatttact ttttcgcctt ttttaaaaac taaaagatat cctacaccta
     9661 aaatcatagc agatcgaaca atatttgcta cgccatcgcc aactaatgta ttaggaatga
     9721 atcccacagc agtatcacca aatgcacgaa taaaagtaat cagaaagata ggtaattgta
     9781 gtccgatcag agatgttgta acaacaccta atgaaattgc catcacacct gctcctgatg
     9841 caagtgatcc tgttagaact acttcccaaa tccgaataaa ttcacgttta aatgctttca
     9901 cgatattcac attgaacatc gcccaagcac atgctaagta aagaactgct aaaacgattg
     9961 attcagcgtt catcgctagc cacgagccaa acttacccat cgctccatga ccaactaagg
    10021 atgctgtacg ccattctgtt tttactgttg acatattctt agcactgcct tgaattcctg
    10081 aaggagcgat attatacgca taaaacttag ctcctgatgt atcaagtgcc gattggagca
    10141 ataaagctac cgactgtgtg ctaaaagttg cattgttagc aacgccccaa ggttgagtag
    10201 catcaaagtt atgcgctgtt ccttttccat caggcttgcg attgtcgtct gtcacatttt
    10261 gatttgctga ccaaatatat ccatttaaat tggttggttt cttaattgca cttccttctt
    10321 ttccatcccc taaattatag cctgtccaaa cagcaggcgc tcttcccata taagcatcat
    10381 tttcggcgac attaacatca ccggcataag tattaatatt gaaagtatca ttcgatgacc
    10441 attcttttaa tagagccata ccgactgaat tagaatctag atcattattt tgtctggcat
    10501 aactgctagt attgatcgct cttatcattg cagatttttt agatggttca taatctgtat
    10561 caacgaactt gatttcggat gtaggctttc cgtcaggtac atcaccacca gtatcagatg
    10621 atggagcaag attttgagta gaagcccaaa gtcttgtgtt aatgatgtag tcagaaacaa
    10681 catttgttgg tatatctcca tctttttttg ctgactggat gccgttgata taaactccca
    10741 atagcgatcc tgccactggt aagaatataa acaatacaaa aatacgaaca atccattttt
    10801 caagtactgg tgataatccc ttcgagttaa caccatcttt cgacaataat ttcattcctt
    10861 gataacctag tattgctgtg aaaaagagca tagataagct tgctatcgct ttaaataatt
    10921 cattatttaa tcccaaattg ttaagcaagc tagttaatgc tttagatacc ggattagata
    10981 catccgatcc accttctccc caccccataa gagaaaatat attcatactc atgaaaaatt
    11041 ttccaaggag ttcatttatg gattcagcaa tattagaaag gattaaacta attgatccga
    11101 taaaactacc aatcgtccta ccaatacttc ctagaatgga tgatgcttga cttgcaatag
    11161 catttcctga gactgtttca atatatccgt agtaactata agttgctaaa gtagatgcta
    11221 gttgcgtacc tgcattaccc cctgcaatcg aattaccttg tttagaaatg tcagaataag
    11281 ggaaatcggc actgatacca ccttgtccta acaaattcat ttgtccagtt ttagcggcac
    11341 tatcattgac ttctttatct cccattagga tacgagcata tagtgtccac attccttgtt
    11401 ctgccggttt actttccgca taagcaacgt tcttatttat aactgtttct tgtttgctat
    11461 cttgattgtc aggagttgga tcattcattc caaaaggagc gcctaaagta actgctaata
    11521 ctcctccaat aactagacct cttacgaccc atcgttttat tctaaggttt tgtagtaaat
    11581 atttcttgaa tttcatagct acgcccctcc aatcgaaaat acaatcttgt tttctttact
    11641 ttcagcaacg gaaggctttg caataatttc agcattagaa tcaccttttc cttcaaacgc
    11701 tccggcttca ctcaacttaa catcgaatat ttttgcgttt ggataagtaa ttattgcttc
    11761 agtaatcgct tcttcaaact cagattcaac taaatgcaag atattccttg caccccctga
    11821 tttagaatcc tcttgtgaag ccatgtcaaa aactaagtgc atggagagat cgtctgcata
    11881 aaaattttga ccatatactc tttctttctt aggtggaaga cgtaaatcta tatttaaatg
    11941 ttttcgtact aattcagcac tcttcaacat ttcattataa gcaatttcca gaagagtttg
    12001 ttcggataaa gcccaaaatg gaactattcg tccaaatcgg ttaataactt caggctttag
    12061 accttcacca atcaaggctt gtttgatccc tgatgcgact tttcggtatt ctctttttag
    12121 ttcagtgctg tattcattag aattttcctt gtttgtaaca cgagatagtg agctatatcg
    12181 gtttaacgaa tcgaacatct ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga
    12241 caatgaaacg gtgtcgtgaa cttctcgatc atcccatcct ctttcagtaa aacttatcct
    12301 accttcatct aaaattgata aaaaggcatc gagtgtttct acaccagctt tctcatattc
    12361 atcacacaca ataatcgcag aaggatcatg ccttacatga gtacctataa gtcgtctaaa
    12421 acgcattgca tcatctttgg ttttgtagtc cggcatatta actaagagaa ctttattttc
    12481 atcgccataa agtcctcttg ccacggcttt agccattgca gttttaccga cccctgttga
    12541 tcctgtaaac agagcagttg cttttggttg ctttcgacct ttaaaacgat caaatccaag
    12601 cttcatacgt ttaaacaaca ttgttatagt ataaactgct aacggttgtc cttttatgga
    12661 ctttaattcc tcttgaagtt tttccatatc aaaatcagaa tctagctgaa tattatactt
    12721 accaaatatt tgcaatagac tttctcgatt ataagcattt ccgtctactc gatcttttgc
    12781 aatcaaatcc tccattacag caattgattt agccggttcg gacaaatcct cacgataaag
    12841 tttgtttgct tgtacgattt cttgaagtaa ttcatcagga atcctttgcg ataatctaaa
    12901 agaagcctga ggatcatctt taacagagga tgtatttagc cattgtctca aaatatcgac
    12961 gacaacatcg ctttttagtt cattcaattc taacggttgt agccgtctcc ctaaggcatc
    13021 atctcttttg atatagtttt catattcttt tcttgtagta gcggtaatca tttgaagtga
    13081 ttcatttgct aacggttctt ttagcgaatc acccccaatt ttattgccac tcccaaaaga
    13141 gctaacaact ttatgaaact cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc
    13201 ttttaaaatt cgctcatatt ctttcaatct tggaattaat gtttctaaac gtgtttggag
    13261 gacatcatct cccccaacac gcaattgtcc gattgataat ttatatactt gtactttctt
    13321 tccatcttgt cgctgtcttt cagcccattc acttgcaagt gctgttttac ctgttccggc
    13381 ttcacctaac aagatagctt gcggtgtttc cggtctattc aagatcagtg acaactcatg
    13441 gagttcgtca tcacgtccca ctaacttatg ttttggtggt ttcattggtg tgagagcatg
    13501 attaaattcg gtttgaatat tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc
    13561 acttcctcta aaataagaat aatttacttc tagtcagtca aagttaggta cttaaatgtc
    13621 gtcttcatgt ctccaaaaga atctttgtta aagtcagcat ttttgtattg atcaatcaat
    13681 ttttgtgtat cattgattgc tttaatttcg gtctccattc gactgtctag ttgacgaata
    13741 ctttcttgcg aatctttatc gtcaggattt tgttgtaaac gttccagata ctcagctttt
    13801 gtggcttcaa ttttcttgat gttagtattt gaagcattta actgatcttc tagcttattt
    13861 acaacattat tcttacttgt aactgctaca acttttgaat aatcaatatc gccatctttg
    13921 gtaagaaagc ttccttgata gacattagtt gaatctgaat atggatttat tctgaaattg
    13981 atattatcag gagtattaac aggttttgct tttttcccaa aagaaattac tccttcatta
    14041 ttaacatcat tcatagaata ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca
    14101 ttgactttgg tatcatcgtc agctgtatca tcaccagaaa ttagtgatac ttggtttgca
    14161 ataaaaactt gataagctct ttcctctaat ttacctttta ggaataagaa agcatcccca
    14221 tcagtactta acatcccata gctcatttca agcttcttag gacgatcttt ttcttttgtt
    14281 gccatatata agttatagtt ttttccttgc gtagataaca actttctagc gttttgggag
    14341 taaccaagtt ttactactgt tacatctcta ttttgatctg tccacacatc tgaaatagtg
    14401 atttctgctc ctgatcgagc aaactccaat gaagacccta taggtgttgt attgttcacg
    14461 agataataat tatagtttcc taattgataa atgtagctac ccaagcctgt taataaaaaa
    14521 agcactccta gaaaagttgc aagcataatg cgtaatccgt gacgtgttct cttatcctta
    14581 tcaccaagca accctccaat aatactgtat tgatctttgg tagatgttga attgacgttt
    14641 ttttccatca gtttgtctgc cccctttcat aaaagagcaa acaatcagac gttttttcgg
    14701 taattctagg ttcgggattt tgaatcattt cggtatattc ttccccatta atatttaaca
    14761 caaaaattat cttcctttct tcgttccttt cttttgctct ttattttcca aataatcaat
    14821 gatttttgct attgtaccaa gatagtaatt cccctcagct ttgttttttc ggattttgct
    14881 aaccattgga gtaggtacaa tctcttcatc tctcaattca ctgaaagatt ttttacctag
    14941 atattttcca ataacagatt ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt
    15001 gccttttagt tcatggttca tattgtcaac gacttttgcg gtttcaataa ttgttttagt
    15061 tctcagctta ataatttcat ctggaatttt caatcctcgt aatttcatcg ttgtactaac
    15121 tggtaaccca tgctttactg catcagcaat actaaaactt tttagatcat ctagtaagct
    15181 ctccatatag ttaacaaatt catgccaatt aatagcattc attgtctcac cccttcattt
    15241 ttttaaaatg aggttcgggc gtaacacatt tatgttatta taatatcata ataacataaa
    15301 taaatcaatc agcaaattta gatatccctt aaagtatcag caattattca acaagatatt
    15361 tattttagct atttcagtca tcataaaaga ctaattagca ataaaataac taatattatc
    15421 catttatcag acgaaaagaa aaaacttatc taattactct atattaactt ttaacaaata
    15481 gttgacttgt ccaaaaatgt taccttcaat ctcacaaaga tattaagatg tttcctacca
    15541 aaactgatat caccggactt tttattctgt gttgtccaaa agtgttacct ttagtcgatc
    15601 aacaaattaa aaaaaattgt ttttttcttg acttgtccaa aagtgttacc catagttgtt
    15661 aaaatttgta tagattagcc attctttcga atattattta gactgaaact tgcttacaaa
    15721 aaaacttctt gcatttattg tccaaaagtg ttacccatac ttttttaaag aagcaggttg
    15781 tattaaaatt acaacctgct tcttctcaat cttcttctaa ccaattatgc attgaaatgg
    15841 ttggcaagtt tgattctggc tcactaaaag tgaatctgag ccgatcaact cttcttcctt
    15901 tctttgcata gattttttca taatcaaaag tttcaaatac ctgtattccg ttttcatcag
    15961 gagctaaaaa ttcttctttc gcctgctcta gtattctttt gtcaatattt gtcattctat
    16021 aacttgaagg tatacttaat gctcttctaa aatcttcaac tttgactacc caataaccat
    16081 ttcgccactc tttatttctg tattgcatga gatgacgata caattctttt gtgtagctac
    16141 ttcttagttt tgtaaaatta tcaagttcaa aacgagtaaa gtttttgcca attgagttta
    16201 agataaattc aaattcctca tttgccttaa ttgtaactgt ttccttctgt ttatctatcc
    16261 ggaatttttg aaaaagccac agctgttcat agacgtctga atcttcaaaa cggtaggtta
    16321 aagtacctag tttgtcactc aaagctttta acacagaata aaaactgtcc ttttctcttc
    16381 tgtcgtattt gaccaaataa cgaaagaggt tgaaatcaaa agttacttct gctgttcctt
    16441 gacgtttcat ttttgataaa atagcccaaa acacatccat ttcaacaggc ttaaatcctc
    16501 gcaaagctgt tgtattcaga ccattgttat aacgcacaat tgcatttgtt tgttctgata
    16561 ctaaatctac atcactatca ataatctttt cggtaacttc ttgagacatt gattactctc
    16621 caatcttctt tttgcgctcc tttaatcatg ttaatctggt actaattaaa ggaaacaatt
    16681 ttttctgctt gttacctaaa ttaaatcaca actaaaggta acagtcaaca aagatttgtt
    16741 actaatctaa ttttctattt tttcgccttc caaatcaaga aaaccgaagg taacgtctta
    16801 tcctagtgtg atacctcaaa tgtccacttt tgttaccttt gctgtccaaa agtgttaccc
    16861 ttactgtcca aaagtgttac ctttactgtc caaaagtgtt acctttatac ccctttaata
    16921 cttactctcc caagagatta agcctcccct aacagatata ttaacagata tttaacagat
    16981 atattaacag atatttaata aaggaggatt actcctctct tatttcaaaa tcttaaaaat
    17041 aaaaaatctt aaaaaaaact aacactatct aggagtttat tttgcttgtg taactttgaa
    17101 taattgaaaa aaaggaaaag gaggaagctt cagcttcccc ttttatttat atttataata
    17161 tatattttaa tctttaaaac tttagcaacc ttcaaaaagg ctttatatca atcttaaata
    17221 gattttaggg tggacataaa gcgatgcgaa catggacaaa aagcgatgtg aatgtggaca
    17281 aaaagcgatg tgaatgtgga caaaaagcga tgtgaatgtg gacaaaaagc gatgtggtat
    17341 tccgaatata tccaaaaaaa gaaaaagtat gtgaatttat ttctaaaaac tagaaaaata
    17401 tttgtttttt aattttggta atttttataa tttcgtattt ttttgaaaag gatttttata
    17461 ttgaaaatgt tctaaaatag cgatttctgc tgtcttatca atgaaaattg attaaaaaaa
    17521 taaaatgtgt tttacagaga ttgagttatg ttataaattt acgatacatt tcttattgta
    17581 tcgtatcgaa aacagatcta tacttattca atggaatcgt accagttcca aatttttaag
    17641 ctcgtaccaa gcttagaaag aagaggcaat cgtatgaaaa taacagaaaa gaatgtaaac
    17701 ccgaaatttg aacctaccga aagcaatcta aaaaagtttg atttaactgt tactttatcg
    17761 aatgaacttg tagacagcat tgttgaaatg gatgctatgc cacaaaaaat ttatctttat
    17821 gctataggac atcttgaccc taaaaatccg ccttttaata atactgttaa agtatctaaa
    17881 aaggctttat ttgcgttgat gtcttctagt tcatcttctt caaaacattc aagattaaaa
    17941 caagttattg aaaaattgca tcagcaatct gtcattaaaa ttcaaagaac taaacctaac
    18001 ggtaagactg aatacaaagt catctcaccg ctagaaatta ctgaattcaa tgatttttct
    18061 gatgcagtat ctattaaatt tagtagttca attatgcctt tgttaacgaa cttagaggat
    18121 cattttacac agtatacatt tgatcaagta gttaacttga caagcaaata tgcgatcagt
    18181 ttttttcaat tattcactaa agattttaat ctgtatgaag caaatagaga taacttaaca
    18241 tggagtacaa aatcacttga tagattaaaa aatcctgttt tctccattgc agatttacga
    18301 aagaaattta gtattgagaa aaaatataaa gcttttcgag actttgaaag acgtgtaatt
    18361 aaagaaccga ttgcccaact gaataaatac actgatttta aaattgacta tgagcttatc
    18421 aagaaggggc atacttatac aaatataaag ttcttgctta cacgtaccat gtcagttccg
    18481 gtaccttata agcaagaaca aagagaccct gcttatttaa aaggcgcaga agataaaaca
    18541 gagaaaatga atcaatcgta cttgaaagct atgaatagca aatacactaa attactaatc
    18601 aaaaactttt tgatcagttc tgccgactta tgcgatctta aacttatgag ccgacttcag
    18661 aataaagttt atccgcttta tgatcgttta tccaaagata gaggatttga atcagttgtc
    18721 aatcacctat cttacgttgc tactcataag gaggattaca caataaaaaa tactgttaaa
    18781 tatctcgaaa aatcaattac gagttatttg ccttatgtga acctttatga ttaacaactg
    18841 aaaacatgcg tgaaggaagg aaaaactttt gaaaaaagat gaactgacaa agtacaatga
    18901 ggagttctta gcaacaattg atatgaaaat ggatttaaag tcgtatgaac tttatcaatt
    18961 gctgttcagt tatatctcac ccacaaagct aaagaaaaca ggcgtagtag atttacctgt
    19021 taaagcgatt attaattaca tggatgttga tcaaaatctt gaaacggaac taaaaaccat
    19081 agttaacact ttgcaaagac aagcagttta tgaaatatcg aatctagaca agaaatataa
    19141 gacgtatact actgtatctg ctattgatcg gattcattgg gatttagaag agcatagtgt
    19201 atctttcagt ttttcagaag atgtaattcc cttaattgtc tcaatcaaaa agaagtatcc
    19261 gttctttgtt tttaaagact tagcatcttt tcaaaaaaaa tatagcatcg tgttatacaa
    19321 atatttggcg atgtattata aaaaatatct tatcgccaaa aataaaggag attttgagaa
    19381 agctgaaata tataagaatc ccctgattga aatagttaaa attcgtgaga ttacgaaaac
    19441 agaaaatgag tataaaacaa tatcgaattt tgatagacgt gttttaaggg aatcagttga
    19501 ggaaattaat gaaaaatcaa tgctgaagat tgattttgct aaagttagaa aaggcagaaa
    19561 ttacaatttt gctgtccagt ttaatatttc tagtgagttt gatgaaaaac cttatagaga
    19621 aaaaaaatta ggttaatgca aaatatatct tactcgtttg aagatgcttc agacgtatgc
    19681 taagatatgt agtgccttgg gagcaaggta ttaatctcct ttagaggtta attagcttgt
    19741 gatcgcctat caattttttg gtaggcgatt ctttttttga aaccaaaaag caaaaattat
    19801 tttttaatta gagaggtgca atgcgatgaa atactttttt tgtgcagtat tcgaatggaa
    19861 aggaaatgaa tatgcagtta aattcccaga ttttccagac ttatatacgc atggtgaaac
    19921 tttagaagat gcttttgaga tggcgcaaga agtattggaa ggatacttgg tgcttttaga
    19981 gttgcaagaa aaagaaattc catcgcctac gcctgttcaa gagttaaatg caagaatcaa
    20041 gcgatctgag aatgttaatt tatttgtcgt aaaagctaat acgaaagttg ctaaagctcg
    20101 aaacaagcaa aattatgatc gaaaatcagt gactgtggaa cactatttaa atgttttagc
    20161 ggctgaagag aatattaatc tgtcgaaagc tttgaatgat ttgttgagag aaaagttgat
    20221 ttgatgtctc tcagatgatc atattaagca aaaacagctt gttttaggac gtatggattt
    20281 aagaaagaca aattatcaaa gaatatatta aaagctctca gaagccttct gagagctttt
    20341 aaattgctat gtattgtact acatctgctc aaattgattt tcttttcatg cttaccatgt
    20401 atctagtttt ccatcaagta atttttagat atgtattgag taataaatag caaatttgct
    20461 tgatataagc tcgaattagg tgctttgtaa tgtgttagga taaattatac aagaaaatgt
    20521 caaaagctct cagaacgctt ctgagagctt tttatcggta ggcttgttta acgtgatctt
    20581 gaaagatacc cttttgatgt ttctctcgat ccctgcatcg ctaattacta agtgttcttc
    20641 aagtgtgatg taggtatata aattaagcat caaaatggtg tagataagca aaaaactccc
    20701 taaaactaag tgttgattat aaaaatctga cttataaaaa agaactgaga atgttagtgt
    20761 tgcttcccaa aaaagaaaca aaattttgta tttccaaaga tagtggatca agcgctgtgc
    20821 tttactcatc tcaaataatt tataataacc tgctaattga tcagcaagcg tatcgttaac
    20881 ggtttctgtt tgtgtttgtt tcatctttct cttctcctgt ttcaattaat ctaaaatttc
    20941 agattgagca agcaactcat tgtggtagtc ggtttgaaat tcaaataaca attttgcctt
    21001 cttaacgagg tttaaagcgt tcgttagtga ttcacttaat gcttcaacat ctttaactga
    21061 ttttgcctgt ttcatttttt cagtgtgcgt atagcagaat tcaggtattg agttaggggt
    21121 tgcctgttca ataaacatct caattcgcaa gttattatcc tcaaaaataa ttagatcatt
    21181 ggcaaagtgt ttggtactaa cgctaatgcc agttgtatct tttaatagat tattgattaa
    21241 cgttcgagag aaatagttta agtgtgtagc tttcataggt tcggtctcct ttttactttt
    21301 caaacaactc ttcttctagt gctttttgaa gtagttgaga gaagtttatg tttttctcct
    21361 tccctaaaag aactaaccat tccggaacac taacattttt tctaattgtt tttgtattta
    21421 agcttcttcg gaaaagttta atgttgagtt ggatgattac taccacttga ttggctttca
    21481 aagttaattc ttccactgct gatgttttag gaaattctgc atctggtaca ctatatagcg
    21541 ctaatcctaa agcatcctta gcgtttaaat atgcttcttc aagactattt ccaaaggtgg
    21601 atatttccgg taagtcaggg aaaacgatgt tataagcttc tccttctttc tcgaagattg
    21661 ccggataaaa aatatttttt gtcttcattt cctcgctcct tttctagtgt aagagaggat
    21721 agtatgccta ataccaaata ggaaggcaag gctgttaaag ccctgcttgc ttccagatat
    21781 tagcttctgt tcctagtggt acatcaccat tgtggatcgg aacaacgacc gtttttttag
    21841 tcttggaatt ataaaatttt gcatgactgc cagtttgtcc agtctttttg aagccgttag
    21901 atttgagaat ttttataatt tctcttgatg tcttaggcat agctaccctc cctttacaaa
    21961 attcattata cacattgttg tgtatggaat caatagaaaa agaggatgtt ttattgcaaa
    22021 actaataaaa tgatttattg gttttttcag caaattgaat gttattagcg ttgcaaatat
    22081 tcatgatttc ccaagtatca tcagaagtat aggtatctaa gaattcattg acagttacgg
    22141 ttgattcgta tggttcataa gtcaaagcgt tctccaagtc ttttgatgaa ataatcaaga
    22201 ctttttcaat ttcctcccct tcttcatcta tgaaaacata ttctaaagtg atttgttctt
    22261 catcggtata attcatattg tgttctcctt tcttgtacta agaaaaatgg atatttccaa
    22321 ttgcatccaa ctcggcacca ttgtggtata ggacgtgacc gagcgttacg cctttgaaat
    22381 gttcttctaa atacatccaa acgcattcaa ggctagtacc tgctttgaga ttgtagaagt
    22441 ctgcatccaa taccttttca gaagttatcg gatcaatgcg agttggtact tcaataaatt
    22501 cagtccaaat ttctgcaaaa gtcttacctt ccaaagtcca cactccctat caattaattc
    22561 caaaagcaaa tagagaaatc aagcgaccgt caatagtcac ttcaacaact gaaaatgccg
    22621 ttgaaacagt tttaacatct acactttcca agttcccgat gaaggaatct ttgtgcttta
    22681 gaatagcttt ctttagctgt ctgttatacg cttgccgact gttaaacttt ttgaattctc
    22741 gatccttgat cagctgttta acgatccctg cattaattgt ataactttct gcaatagcaa
    22801 cgtgagtagg ttgctgtaaa gtagctgtta gcatcttgtc actccttcct aaaagttaat
    22861 tagcttgttt acaaatacat tatacacatc ggtgtgtata atgtaaagag aaaaaggtgc
    22921 taatggatca gctgttctaa acaaaagaaa acgataaagt aacaaataaa gtcagcaaag
    22981 ttaaaaagga tgaaacggct agtccggttg ctttgcttgt gtgattttcg gctttcttat
    23041 agatcaaata ggaaattatc agtgatattc cagtaaagaa taatgtacta gctgtcacta
    23101 aaagaaatag gttaatcata cgatacactc cttctgtaat attggtatca gatacgttta
    23161 aatgcgtttc tgagctgttt tggatcagat ggtgatgttt ttagccctac cctaaaaaca
    23221 aagcccagaa aagaatttaa aagtgttcgt acctaatacg tttatattca tatacatata
    23281 cataaacata aacgtataca cgtttatgtt tatgtattta agttttaggc tacttcctta
    23341 acatctcctg ctgatagtcg agtgaaccga gtgagtgatc catcttgtag tcgaaaaata
    23401 aagctacgtt tgagtttata ctgatagaca gcgacatacg atccatccca gattgagtaa
    23461 gttttacctg caactaataa gtcatcaagt tttggtttat tgctttcttc tttaattcta
    23521 tctagttgtc gcttaaccat tttttcttga tcaccgacaa agaatttcaa ccattttttc
    23581 ggaattttct taaagtaagc aggactgtca tttaagaagt agattggttt gtacatttca
    23641 tctcgaaaga tgaagctgtc aatatcgtag gttccttctg catctttgaa aaggataatt
    23701 ccataggtct tcgttacttt ggtatctacg atttccaaac catttttggc gtaccctttc
    23761 ttttcttcga tcacttcatt tgctgatgaa taatcaccat accatcccat aaaaaatcgc
    23821 tccttttgaa agttaattag cttgtttaca cttatattat acacaccggt gtgtataata
    23881 tcaagaggat attgttcttt tttaggtaaa gtgggatcgt aaacagtgat tttgttgggt
    23941 taacaaaaaa acagacaagc cttatcagct tgtctgttac ttataaactt cacttctgtg
    24001 accaatagac aatataagaa ttgtcacttc atgctcgcta atatcagcta ttatacgata
    24061 tgcacctacc ctatatcgcc agtagcctga tttattagct gttagacctt taccatgctt
    24121 tcttggatca tcagtgccta caagattttt ctcgatccag tttaggataa cctttgcctg
    24181 aaacttatcc atttttttta gcgctttttg agcttcacgt tcatatctta cttggtaagt
    24241 catcttataa gcctaactct tttagcatat cttcgtggga aatactttga tcgttttcct
    24301 catgctcttt cattagttgc ttgtaggtgg caaagtctac taacgtttct gctgtttgca
    24361 gggaattact acgcataaat tcagaaatgt tatctccgtt gaattcagca actttttcaa
    24421 gaaaatcttt ttcttcttca gatacacgaa tacttattac tggcatagtt tatcaactcc
    24481 ttttcaatta cattgtacta cgattgtatg acaaatgcaa tagttatatt tttgaatata
    24541 tacataaaca taaacatata catatatatg tttatgttgt ggaatataaa agcaaatata
    24601 aatataaaca catttatgta tataaacata tacataaacg tgtttatagt gtataatgga
    24661 gttacaaaag gttaataagg gaggtttaat catggaaaag aagttgtcaa tcattactgg
    24721 aaacattgaa aaaggtggaa caggtaaaac aacaacaatc tacaatctag gtggatatgc
    24781 tgtcgaaaat ttaggtatga aggtgctgta tattgatgaa gataagtcgc aaaacctatc
    24841 aaaacgattt cctgatgctt tcagtaaagc gaaaagagag aatacaatca atcatttata
    24901 tcttcatgga tcagctgaac ctttagctat aaatgagaac atggatattt tagtagcagg
    24961 tgaagattta aaacaagttg agatgcaaat gcgtgatatg cctaacaaca gaaccctact
    25021 gtttagatgg atcgttgtta actatgaaca gcttaaagag aagtatgatc taattctgat
    25081 tgatactcat aatgatgcag gtctactagc tcaaaatgct tgggctgtat ctgatcttgt
    25141 cgtaggcatt tctgatccat ctaaagatgg ttttgaagct ttgctgaagc ttggtaaaga
    25201 tattgatgca ctagcaaaac agttgattga tattaagact ggtgaaacac tgatggaaac
    25261 taaatacgct acgatcggta gcaggatcaa acataatact aattcaagtc gtgagttcct
    25321 agaattggta ccaacgttat ctaattatct tggatatgta caagagaagg aattgcttaa
    25381 tgcaactagc ttagaaggta agaatattgc tgagttcgct aaggataaga atatctatcg
    25441 taagaataag gatttctttg aaagcacgtt tacgctatac aacaaaataa ttgatacggc
    25501 acaagaataa ggagtgattg aagtgaaaga aaatagattt aaaaaattcg ctgaagctga
    25561 agtagaagaa gttcctgaag tggttgaagc agttgaagaa cctaaacaaa cacctaaaga
    25621 aacaacaagt tctttggtag ctaaactaaa aagtgatagt tctgaaaaga agaaaaatta
    25681 tcaattcacg cttcaaccat cacaaagaaa gaaactatct aaaatcagcg aaaagttagg
    25741 aattcgatca gattcagctt tacttggtga aatgatagat aacttgtatg atgaagtttt
    25801 tggtgagtga tcagtttaaa gctgatcact ttttttattg gaataagcgg ttaggtatag
    25861 cttaactaaa tttcgtatac gtgtttatgg atatatacgt atacatgttt atatacatgt
    25921 atacgtatat gtttatgttt gtatttgtac ataataaaaa ggctaggtat tgtaacctcg
    25981 cctttttatt tttaaagtaa gtctttgtta cttggtgcat tgaacccttt tagcattggc
    26041 ttttctttct ttgctgaatg atcggatgta accgttgaag gatgctcttg ttgaataggg
    26101 ttatgatcag ttggtttatt ctttacttct gctactttgc tatcgtttct atcgttgcct
    26161 gcttcaataa ttgtaacttc attgttcttt acacgccgat atagattcaa agcttttttg
    26221 atcgtctgag agtatttaac gcctagtgtt tcatattcag attcaagaaa ctctgctaaa
    26281 ggtaactcgc taaccttttc tctattgatt ataactacat aacgctctga ttttccctta
    26341 ttcatgttga tctccttcgc tgttttattg agttaaacca aacttgtaaa agccttttac
    26401 attagaaaat tcactgtctt caacaaacaa ggtatgcgga attttatctt ttacaagttg
    26461 tttgtcgata atagaagccc caccaccagt aaagacaact ttatctaagg ttgtgaagtt
    26521 tttgaatgta gctaaaactt ctgcaacaac catattggtg tatgttttta tttctctttc
    26581 aactacttct gaaatatcaa aactttcatt tccgcttggc ttaaatagat atacgccttt
    26641 ttctttgcct tctcggataa tcttttcaag ttggaaagca ttaggactga tcggaaggtc
    26701 aagcatattt acgatacgat tatataaagt aaaaataccg gtatcctttt gttcacggtt
    26761 gttttcgtca atttcaaaat tttggacaac atcaaaaagt ttcgtgccac caccaatatc
    26821 aacaaaaccg atcttctgac tgatcaatga ttcatcttgt acactaccat catcagcaag
    26881 gaggaggtta tatagtgttc caatcggctg tggcagtacc caaacggtat ctacgtttat
    26941 attataagct ttaccgtcaa cttcaactga atgttgtcgt gtggcttgtt gcatcacgta
    27001 tttgacgatt gtttcggtat agtcggatga aggcagacct aaaactagat taacggttaa
    27061 aacgtcatca gattgatctt ctaaacgtgc taggttagct aaagagaaag ataggatatt
    27121 ttggtaggtt tcctgcttgt atctgctgat cccaaaacct agtgattcta tcacgtagtc
    27181 atcttttcct agctcgtgta tatccttccc aaaataatag ctttcagtag tgtttactag
    27241 gctatattca gaaacgttta gtgatcgttt taatgaggaa cctatattag aaaaacgtcc
    27301 tgaattaatg atatttttgt tcaaaatacg agagggaagc gagagtggag aaacgtgtgt
    27361 actcatttct tttactgatt tattccctaa gtcaacggaa aacaataatg gttctttcat
    27421 ggtatgacct ctttctgttt gtgtttatgg tatttcggta cgaaaatagt ttacacctat
    27481 ttactaatag ttgcaactgt ttataataaa aaaatgcaat caaaaaagta taatcaatta
    27541 caataaagtg taagtaaaaa ggtgtaaata aacacttgca aatttatctt attatgatat
    27601 tataaaaaca taaattatat taaggagtga tcctgatgcg attgagaaga aataagaatt
    27661 acactactat tattttagaa gattttactg atcaagaatt acttattgtc ggtaaaacaa
    27721 aagcagattt tatcgaaaag ggaaatgctt caatagtaaa agcttcaaag gttgctgtcg
    27781 aaaaatattt tgattcttta gaagaaagtg ctaaggaaat ttttaatgga gagaaagctg
    27841 gtaactatga gttagatggt attaaattta gcttgatccc aacaactgaa aatattattc
    27901 cgaagaaatt atattatatc ttttcatctg aaaaacatac ttcaatgaag ccttgcattt
    27961 ttgattggca atggttgatt gatgctgtat tggatgtaaa atgttcaaaa tacaatatta
    28021 ctttggggtg ttaattatga gtatgctagt tagaaaatta ccattatctg taagagaatt
    28081 tctattttac ttggtctgct taatcgcatc agggtttaag ctgatcgaac atatttttat
    28141 ctttgcttat ttattagcaa tgatcgcatt tttatctggt ggttggttaa ttatatattt
    28201 gttaagctaa tttttagatc aaaaataatt aataaattaa acgataaaat aaccgttgga
    28261 aaatgtaatt caaggacaac acctaacaat cagtttgtaa tcaaattata aaataacata
    28321 attcaataat taatgctttt taaaggacaa attaagtgta tcaacataaa cgtgtatatg
    28381 tatatgttta tgttgatact ttttttgtct taattttttt attttttaaa ttctatttac
    28441 taatgctgtg taggtagtga tcagaaaaaa caaaatagcg aggttaagct atttattagt
    28501 tcatatatta tattcagatt aatatgatcc aatattaatc tgaaggtgaa accaatatat
    28561 ttctacggtc tcatgtaaaa ttatttagat gttcctgcta ataaaataac aaaaataaaa
    28621 taatcacctg ttttatacca tttttcgata acattattct atttgtaata ttaaaacatt
    28681 taaattttga attatttttc taaattagat caatttatat tcttataaaa cagtataaaa
    28741 aatggttttt atatcgttat ttattgatat tattctcata aaaaacaatg aaagcgcata
    28801 cttttatgaa tggttaaatg taattttaat acctatacca attaaaagag aatttttcat
    28861 attttcactt aaaaatttat aaaaaaacaa gataattgat acttcgattt tgcgaacatt
    28921 ttgcctttcc gcctaattca gctttaatat catgattata tcggcaacaa atcgcttttt
    28981 tagataattt aagcatcaaa taattatcct taatcagggt aaaaaataac gaataaatgg
    29041 attttgggaa taggtaaaaa ctagatcggt ttctgacgaa aaataacatg aaaaaggaaa
    29101 tataaaataa catggagcgt gggatggata aagaattttt acacgcaatg tgcgtagtaa
    29161 tgtaaagaaa cttaacacaa aacaaaagt
//
LOCUS       DACEJR010000037        29109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEJR010000037 DACEJR010000000
VERSION     DACEJR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.037"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1030)
                     /locus_tag="IW651_001629"
     CDS             complement(<1..1030)
                     /locus_tag="IW651_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0814354.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IW651_001630"
     CDS             complement(1112..1801)
                     /locus_tag="IW651_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0814355.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW651_001631"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW651_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0814356.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IW651_001632"
     CDS             complement(2328..2831)
                     /locus_tag="IW651_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0814357.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW651_001633"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW651_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0814358.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW651_001634"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW651_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0814359.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW651_001635"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW651_001635"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0814360.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW651_001636"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW651_001636"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0814361.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IW651_001637"
     CDS             complement(6443..7462)
                     /locus_tag="IW651_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0814362.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IW651_001638"
     CDS             complement(7635..8522)
                     /locus_tag="IW651_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0814363.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW651_001639"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW651_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0814364.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW651_001640"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW651_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0814365.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW651_001641"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW651_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0814366.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IW651_001642"
     CDS             9753..10397
                     /locus_tag="IW651_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0814367.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IW651_001643"
     CDS             complement(10453..10956)
                     /locus_tag="IW651_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0814368.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IW651_001644"
     CDS             11553..12518
                     /locus_tag="IW651_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0814369.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IW651_001645"
     CDS             complement(12617..13789)
                     /locus_tag="IW651_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814370.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IW651_001646"
     CDS             complement(13776..14921)
                     /locus_tag="IW651_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814371.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IW651_001647"
     CDS             complement(14935..15867)
                     /locus_tag="IW651_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814372.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IW651_001648"
     CDS             15984..17063
                     /locus_tag="IW651_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0814373.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IW651_001649"
     CDS             17060..17689
                     /locus_tag="IW651_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0814374.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IW651_001650"
     CDS             complement(17799..18026)
                     /locus_tag="IW651_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0814375.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IW651_001651"
     CDS             complement(18129..20717)
                     /locus_tag="IW651_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0814376.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IW651_001652"
     CDS             complement(20737..21396)
                     /locus_tag="IW651_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0814377.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW651_001653"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW651_001653"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0814378.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IW651_001654"
     CDS             complement(22169..23035)
                     /locus_tag="IW651_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0814379.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IW651_001655"
     CDS             23144..23785
                     /locus_tag="IW651_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0814380.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW651_001656"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW651_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0814381.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IW651_001657"
     CDS             complement(25226..26125)
                     /locus_tag="IW651_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0814382.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IW651_001658"
     CDS             complement(26127..26924)
                     /locus_tag="IW651_001658"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0814383.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IW651_001659"
     CDS             complement(26902..27606)
                     /locus_tag="IW651_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0814384.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IW651_001660"
     CDS             27737..28312
                     /locus_tag="IW651_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0814385.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IW651_001661"
     CDS             28430..29107
                     /locus_tag="IW651_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0814386.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DACEJR010000038        26174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEJR010000038 DACEJR010000000
VERSION     DACEJR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.038"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW651_001662"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW651_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0814387.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW651_001663"
     CDS             808..1242
                     /locus_tag="IW651_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0814388.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW651_001664"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW651_001664"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0814389.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW651_001665"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW651_001665"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0814390.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW651_001666"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW651_001666"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0814391.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW651_001667"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW651_001667"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0814392.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW651_001668"
     CDS             9513..10439
                     /locus_tag="IW651_001668"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0814393.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW651_001669"
     CDS             10621..11514
                     /locus_tag="IW651_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0814394.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW651_001670"
     CDS             11648..11773
                     /locus_tag="IW651_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0814395.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW651_001671"
     CDS             complement(11907..12539)
                     /locus_tag="IW651_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814396.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IW651_001672"
     CDS             complement(12687..13229)
                     /locus_tag="IW651_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0814397.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IW651_001673"
     CDS             13364..13753
                     /locus_tag="IW651_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814398.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IW651_001674"
     CDS             13757..14521
                     /locus_tag="IW651_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0814399.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IW651_001675"
     CDS             14650..15597
                     /locus_tag="IW651_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0814400.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IW651_001676"
     CDS             15727..16467
                     /locus_tag="IW651_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814401.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKERVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IW651_001677"
     CDS             complement(16758..17426)
                     /locus_tag="IW651_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0814402.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IW651_001678"
     CDS             17560..17847
                     /locus_tag="IW651_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814403.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IW651_001679"
     CDS             18263..19801
                     /locus_tag="IW651_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0814404.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IW651_001680"
     CDS             19798..20763
                     /locus_tag="IW651_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814405.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IW651_001681"
     CDS             20750..22072
                     /locus_tag="IW651_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0814406.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IW651_001682"
     CDS             22313..23056
                     /locus_tag="IW651_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814407.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IW651_001683"
     CDS             23071..24063
                     /locus_tag="IW651_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0814408.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IW651_001684"
     CDS             24075..24812
                     /locus_tag="IW651_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814409.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IW651_001685"
     CDS             24802..25524
                     /locus_tag="IW651_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0814410.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>26174)
                     /locus_tag="IW651_001686"
     CDS             complement(25608..>26174)
                     /locus_tag="IW651_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0814411.1"
                     /translation="FMGTTAHADEAYTVQSGDTLSTISQKYVGDNSLINAIAESNSIS
                     DINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAPVAETQ
                     PAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQ
                     ERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8999 a         4451 c         5460 g         7264 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caaggagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagtaaat caggttaata
    26041 tcagaaattg aatttgattc tgcaatggca ttgatcaagg agttatcacc aacgtatttt
    26101 tgagaaatcg ttgatagagt atcacctgat tgaacggtat aagcttcgtc cgcatgagca
    26161 gtcgttccca tgaa
//
LOCUS       DACEJR010000039        25944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEJR010000039 DACEJR010000000
VERSION     DACEJR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.039"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW651_001687"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW651_001687"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0814412.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW651_001688"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW651_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0814413.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="IW651_001689"
     CDS             complement(3549..4358)
                     /locus_tag="IW651_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0814414.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW651_001690"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW651_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0814415.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="IW651_001691"
     CDS             complement(7968..8654)
                     /locus_tag="IW651_001691"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0814416.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="IW651_001692"
     CDS             complement(9002..10783)
                     /locus_tag="IW651_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0814417.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="IW651_001693"
     CDS             complement(10809..11723)
                     /locus_tag="IW651_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814418.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="IW651_001694"
     CDS             complement(11750..12712)
                     /locus_tag="IW651_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0814419.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="IW651_001695"
     CDS             complement(12712..13656)
                     /locus_tag="IW651_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814420.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="IW651_001696"
     CDS             complement(13657..14661)
                     /locus_tag="IW651_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814421.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW651_001697"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW651_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0814422.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW651_001698"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW651_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0814423.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW651_001699"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW651_001699"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0814424.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="IW651_001700"
     CDS             complement(18496..20178)
                     /locus_tag="IW651_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0814425.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="IW651_001701"
     CDS             complement(20230..20592)
                     /locus_tag="IW651_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0814426.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="IW651_001702"
     CDS             20862..21050
                     /locus_tag="IW651_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0814427.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="IW651_001703"
     CDS             21288..23957
                     /locus_tag="IW651_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0814428.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="IW651_001704"
     CDS             complement(24048..24668)
                     /locus_tag="IW651_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0814429.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="IW651_001705"
     CDS             24780..25181
                     /locus_tag="IW651_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814430.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="IW651_001706"
     CDS             25316..25654
                     /locus_tag="IW651_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ0814431.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7026 a         5500 c         4644 g         8774 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttcc
//
LOCUS       DACEJR010000040        25695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEJR010000040 DACEJR010000000
VERSION     DACEJR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.040"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..3654
                     /locus_tag="IW651_001707"
     CDS             127..3654
                     /locus_tag="IW651_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0814432.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IW651_001708"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IW651_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0814433.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IW651_001709"
     CDS             7442..7783
                     /locus_tag="IW651_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0814434.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IW651_001710"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IW651_001710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0814435.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IW651_001711"
     CDS             9140..11560
                     /locus_tag="IW651_001711"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0814436.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW651_001712"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW651_001712"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0814437.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IW651_001713"
     CDS             12994..13731
                     /locus_tag="IW651_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814438.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IW651_001714"
     CDS             13745..14560
                     /locus_tag="IW651_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0814439.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IW651_001715"
     CDS             14573..15220
                     /locus_tag="IW651_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0814440.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IW651_001716"
     CDS             15236..15892
                     /locus_tag="IW651_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0814441.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IW651_001717"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IW651_001717"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0814442.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IW651_001718"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IW651_001718"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0814443.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IW651_001719"
     CDS             18234..18752
                     /locus_tag="IW651_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0814444.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IW651_001720"
     CDS             18859..19359
                     /locus_tag="IW651_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0814445.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="IW651_001721"
     CDS             complement(19639..20559)
                     /locus_tag="IW651_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0814446.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="IW651_001722"
     CDS             20724..21737
                     /locus_tag="IW651_001722"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0814447.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="IW651_001723"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="IW651_001723"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0814448.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="IW651_001724"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="IW651_001724"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0814449.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="IW651_001725"
     CDS             complement(23377..24138)
                     /locus_tag="IW651_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0814450.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="IW651_001726"
     CDS             24327..25421
                     /locus_tag="IW651_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0814451.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25507..>25695)
                     /locus_tag="IW651_001727"
     CDS             complement(25507..>25695)
                     /locus_tag="IW651_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0814452.1"
                     /translation="NSNGILRLNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8511 a         4454 c         5415 g         7315 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tataggttat
    25501 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
    25561 tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc tgacactgga
    25621 aataaatgtc tgattcactt ttctaaaatc cattgatttc ggcagtccat taagacgcag
    25681 aatcccgtta gaatt
//
LOCUS       DACEJR010000041        25378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEJR010000041 DACEJR010000000
VERSION     DACEJR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.041"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW651_001728"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW651_001728"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0814453.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IW651_001729"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IW651_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0814454.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IW651_001730"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IW651_001730"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0814455.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IW651_001731"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IW651_001731"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0814456.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IW651_001732"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IW651_001732"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0814457.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IW651_001733"
     CDS             5265..5843
                     /locus_tag="IW651_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0814458.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IW651_001734"
     CDS             5895..6377
                     /locus_tag="IW651_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0814459.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IW651_001735"
     CDS             6494..6868
                     /locus_tag="IW651_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814460.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW651_001736"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW651_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0814461.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IW651_001737"
     CDS             9167..9757
                     /locus_tag="IW651_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0814462.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IW651_001738"
     CDS             complement(9885..11234)
                     /locus_tag="IW651_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0814463.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IW651_001739"
     CDS             complement(11402..12142)
                     /locus_tag="IW651_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814464.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IW651_001740"
     CDS             complement(12155..13747)
                     /locus_tag="IW651_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0814465.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IW651_001741"
     CDS             complement(14315..14611)
                     /locus_tag="IW651_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814466.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IW651_001742"
     CDS             14787..15512
                     /locus_tag="IW651_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0814467.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IW651_001743"
     CDS             15505..16347
                     /locus_tag="IW651_001743"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0814468.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IW651_001744"
     CDS             16350..16871
                     /locus_tag="IW651_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0814469.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IW651_001745"
     CDS             complement(17124..17687)
                     /locus_tag="IW651_001745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0814470.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IW651_001746"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IW651_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0814471.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IW651_001747"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IW651_001747"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0814472.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21031..21297
                     /locus_tag="IW651_001748"
     CDS             21031..21297
                     /locus_tag="IW651_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0814473.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21441..23432
                     /locus_tag="IW651_001749"
     CDS             21441..23432
                     /locus_tag="IW651_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0814474.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23786..25087
                     /locus_tag="IW651_001750"
     CDS             23786..25087
                     /locus_tag="IW651_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0814475.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8173 a         4807 c         5203 g         7195 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt aggagctgtt
    20701 tttacttggc tgttcttatt acttgatgca gaacagcttt tttacaacct ctccaataaa
    20761 cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata tgaggaacta
    20821 tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac ctctgattca
    20881 cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac aacttctttt
    20941 tttctggagt ttcttggagc atatttttgg agtccttctg aataaagcgt aaaataaaag
    21001 tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc gttatttatc
    21061 ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc gggaggcgta
    21121 tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt actaggacca
    21181 ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc aattttttgc
    21241 cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa agaataattt
    21301 tattcaaata agttgacact tcagtgtgtt acttggataa ttagaagcaa tttaggaaag
    21361 cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga tatccatata
    21421 acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata tcaatggctg
    21481 gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg ctttacgtta
    21541 tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat tttgttatcg
    21601 gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa tatgtcgtga
    21661 ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac acacttgtcc
    21721 gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc ttactagcag
    21781 atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg cgaggaattc
    21841 cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc actttagtta
    21901 ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga aaagcattcg
    21961 gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg aacttcagtc
    22021 aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg ctttttagtg
    22081 cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg acaggtgcag
    22141 aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg agctggcctt
    22201 acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg ttgaatgttt
    22261 atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa atgatgcctc
    22321 aaagttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt gcttcgcaag
    22381 cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa ctgctgcctc
    22441 ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca gcagtcaatc
    22501 tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct catcatatgg
    22561 aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca ctgctttact
    22621 tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc tttttcttcg
    22681 gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga ggcggttttg
    22741 tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa caagggaaca
    22801 ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg cagcttagag
    22861 aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc ttggttcctg
    22921 atatggctga agataaagtt ggccgacagt tcatctattc gattttagat aaacggccta
    22981 aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca tatacgaaag
    23041 aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg ttcttaggct
    23101 tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat ttgatgaaac
    23161 aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga gaagttggcg
    23221 atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta ggaaaatggc
    23281 aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg ccagaaacat
    23341 ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc attggaaata
    23401 ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg aactaatgtc
    23461 gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta ctgcaacaaa
    23521 cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg ggcttaatgc
    23581 cgcagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc tgtttagcaa
    23641 caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc cgcatctcct
    23701 tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa aagggtacaa
    23761 tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat ttttgatgat
    23821 tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca gacgatcaag
    23881 attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc atgttacaca
    23941 ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg gtttgatcat
    24001 cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg gtaaactacc
    24061 ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt tagttacgta
    24121 tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca atacggagaa
    24181 ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt ttccttttat
    24241 ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc gtctcgtatc
    24301 tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga attcttatga
    24361 aaaaggagag ttgaccaaag aagaatatca ttatttggaa aacgtgtttg aatttgatga
    24421 taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg aagcttcaga
    24481 aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct atccggttat
    24541 cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga tcaagcaatc
    24601 ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg taacaactga
    24661 aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggaaagc atattgctat
    24721 cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata ttttagaaga
    24781 aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag cggaacagtc
    24841 agatggctct tatattatct ccggcaaact aacattagat gatttccagc gttattttca
    24901 tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct ttgcttctag
    24961 ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc gtttcacggt
    25021 attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg aaagaaaaac
    25081 agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttccta cgattagtca
    25141 agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa gcacaccaaa
    25201 taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac tgtaagtggt
    25261 ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt gccctaacca
    25321 tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc ttatcttt
//
LOCUS       DACEJR010000042        24012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEJR010000042 DACEJR010000000
VERSION     DACEJR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.042"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1149)
                     /locus_tag="IW651_001751"
     CDS             complement(<1..1149)
                     /locus_tag="IW651_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0814476.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFI"
     gene            complement(1115..2593)
                     /locus_tag="IW651_001752"
     CDS             complement(1115..2593)
                     /locus_tag="IW651_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0814477.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(2898..4733)
                     /gene="tdc"
                     /locus_tag="IW651_001753"
     CDS             complement(2898..4733)
                     /gene="tdc"
                     /locus_tag="IW651_001753"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0814478.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            5203..6630
                     /locus_tag="IW651_001754"
     CDS             5203..6630
                     /locus_tag="IW651_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0814479.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(6722..7087)
                     /locus_tag="IW651_001755"
     CDS             complement(6722..7087)
                     /locus_tag="IW651_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0814480.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(7084..7449)
                     /locus_tag="IW651_001756"
     CDS             complement(7084..7449)
                     /locus_tag="IW651_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0814481.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(7641..7889)
                     /locus_tag="IW651_001757"
     CDS             complement(7641..7889)
                     /locus_tag="IW651_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814482.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            7888..8016
                     /locus_tag="IW651_001758"
     CDS             7888..8016
                     /locus_tag="IW651_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0814483.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            8073..9767
                     /locus_tag="IW651_001759"
     CDS             8073..9767
                     /locus_tag="IW651_001759"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0814484.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            9771..9890
                     /locus_tag="IW651_001760"
                     /pseudo
     CDS             9771..9890
                     /locus_tag="IW651_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            9972..10163
                     /locus_tag="IW651_001761"
     CDS             9972..10163
                     /locus_tag="IW651_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814485.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            10541..11674
                     /locus_tag="IW651_001762"
     CDS             10541..11674
                     /locus_tag="IW651_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0814486.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            11705..12025
                     /locus_tag="IW651_001763"
     CDS             11705..12025
                     /locus_tag="IW651_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814487.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(12054..12518)
                     /locus_tag="IW651_001764"
     CDS             complement(12054..12518)
                     /locus_tag="IW651_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0814488.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(12649..13906)
                     /locus_tag="IW651_001765"
                     /pseudo
     CDS             complement(12649..13906)
                     /locus_tag="IW651_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(13935..14252)
                     /locus_tag="IW651_001766"
     CDS             complement(13935..14252)
                     /locus_tag="IW651_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0814489.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(14278..14598)
                     /locus_tag="IW651_001767"
     CDS             complement(14278..14598)
                     /locus_tag="IW651_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0814490.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(15062..16477)
                     /locus_tag="IW651_001768"
     CDS             complement(15062..16477)
                     /locus_tag="IW651_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0814491.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(16494..17810)
                     /locus_tag="IW651_001769"
     CDS             complement(16494..17810)
                     /locus_tag="IW651_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0814492.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(17960..18658)
                     /locus_tag="IW651_001770"
     CDS             complement(17960..18658)
                     /locus_tag="IW651_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0814493.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(18947..19936)
                     /locus_tag="IW651_001771"
     CDS             complement(18947..19936)
                     /locus_tag="IW651_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0814494.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(20016..20849)
                     /gene="msrA"
                     /locus_tag="IW651_001772"
     CDS             complement(20016..20849)
                     /gene="msrA"
                     /locus_tag="IW651_001772"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0814495.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(21024..22658)
                     /locus_tag="IW651_001773"
     CDS             complement(21024..22658)
                     /locus_tag="IW651_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814496.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AHQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(22645..23979)
                     /locus_tag="IW651_001774"
     CDS             complement(22645..23979)
                     /locus_tag="IW651_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814497.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         7634 a         4825 c         3992 g         7561 t
ORIGIN      
        1 aataaattga acatgtgcac acaagatcga agaccacaca aatagaaaac aactcgttgt
       61 catgcctgcc aaaagctgaa aaaacaatcg actatcagct acaaaaaaat gtagacaaag
      121 accggcaaaa aataacattc ctgtaattaa aataccgtgt aaaggtacgc ctttcttaga
      181 aagcgcacaa aaccaatggg gcaaactttt ttcttttgct gtttgatata aaatcctgct
      241 agtactgtag actgcactat ttgctgagga caaagaagca accaaaatca ttagattgac
      301 aattgctgct ccgccttggc aatggatcac ttgcagcata gtcactaaag gactttcttt
      361 taaatctaaa caatgccatg gaacaacaaa taaaatcgac aaaatcgttc ctatatagaa
      421 aaagagaata cgccaaggca gacagttgat tgcttttttc aaattctttt gcggatcact
      481 tgtctcgcca acggttaagc cgatcatttc aattccagca aaactgaata aaaccatagg
      541 aaatgagact accattccag caaagccaaa cggtagtaat tcatcgatct gtccaaaatt
      601 ttctttgatt ccgatagact gggtatgaaa atctgagaca ctcaatccta tacccaataa
      661 gataaaaagg aaaatcccca agatcttgat gattgagaaa aaagattcca attcaccaaa
      721 ccacttgact gcaacaaggt tgactgccat taagatcatt agtgcgctac ctccgcttat
      781 caaagctggt acatgtacaa accagaaatg gatatacaat cctattgctg ttaattccgc
      841 aagtccagct ccaagccagc aaagccaata agtccaattg acaaaaaatg cccacttttt
      901 gcctaaataa tagtcaacga aatcactgat agagttaaat gtactatttg ctaaaaataa
      961 atcacccatg gcccgcatca aaccataaat agccaaaccg catatcaaat aaacgagaat
     1021 aacagctggc cctgctgatg caattgcctt tgctgaacct aaaaataatc ctgttccaat
     1081 tgcgccacct aatgcgatta actggatatt tcgattagtt agagtccgtt tcattgccgg
     1141 tttttccatc aagttcacct ttttcttttt caagcagcat ggcgacttcc tgttcccaag
     1201 aaggtttctt tattttttca atacagagtg gtactacaaa caaaatgaca ctgatggctg
     1261 tgactgcaat tagattgatc catgttccag ctttcatcgt cagtccgaca gaaaatccga
     1321 ttgccgcaaa taaaacaata acgacagtcc aaactccaag attgctcttt ttctcttttt
     1381 ttccaattct aaatggtcga ttcaactttg catgtgtata acgcaatctc aaaactgcga
     1441 tccccatgat gacatagacg atacagtaga taacagtagt tgaatttacg agcatcaaaa
     1501 atgcttgatt gacgccagga atcagcaaat aaaaaagtgc aaagattgag ataatgacag
     1561 actgagtcaa aataacggat ttggatacgt caaattgatt cgtcttccaa aaattgaatt
     1621 ttggtggata cgcacctttt ctagcacttt ctgtcactgt ctttgctggt cctgaggccc
     1681 aagcagataa ttggactgct acaccaatga agacaagtaa actaaacaga ttcacaataa
     1741 gatgtggcaa accaagaatt tggcaataaa tcgaaataga ttgcgcgata ttattcaatt
     1801 ccatttgtcc ttttggtacg acatttgcta caactaaagc attcaaaata ttgacgaaaa
     1861 atataaaaat taacgcggca aatactccgc gaatatacgt tttaactgga ttttccaatc
     1921 gagtgatata cacagaggac atttcgatac cagtaaaaat aaacatgatc ggcgcaaaat
     1981 aaacaaatga ggatgcagtc gtagtatctg gtattaattt gtcccatgaa aaactgccta
     2041 atgttccggt tggtacgatc ccaactttga tccacgcagc aaaccctaat aagagcatca
     2101 tcgtcaaagg cacgtacaat cctaaccaaa caccgacttt tccgccgatt tttgccatat
     2161 caaatttcaa attcaaaaaa gtgatgatcc aataaacgac taaaatgaca actaatgtaa
     2221 atttgctgtt caaaccaagc ggcacattat cgataatatt accaaacaac ggagcgagtg
     2281 ctgaagcgac catcaccatc ccaggaaaca tttggaccca taacagccat gagacaacga
     2341 acccccattt ttttcctaga gcattcgata cccaaagttc tggaccacct ttttggggaa
     2401 acattccagc aaattcacca gagatcaatg agattggaaa agcgtacaat aatactgcaa
     2461 ccagcatgta aaaaatcatc gtccagctcg ttgctgctac atctggaata tttcgtacac
     2521 ttgctacaag ggcgcaggtc attccgataa aggtcatggt cgtgatttgc tttgatgaac
     2581 cagtatcttt catctttatt ccttctttca atagcgacat atgtttttgt tttcttttat
     2641 ttttgttcaa gaacagaaat gacaaggttt catcgattca gtacaaaacc cagtatcacc
     2701 gaaacatcca ttgaacgatc ttagtgaact gggtttgata catgaaacga acgatccaac
     2761 ttatagaacg ttttataaaa aagaatatct tggcgaggaa tatatgaaaa acagggggaa
     2821 atacaattgt tttgatgaag caaataattg tctgttctcc atatgtctct tgttattctt
     2881 tcacacaaaa atcagttcta ttggatgatt tcgtttagtt tcttttccat tgctttggcg
     2941 attttttttg tataaaaatt gaaaattcga tcatcattca aataaggagt catgatagcc
     3001 gcacgtagca gagtgatttt ctgttctttt ttccattctt ctttcgttag acccattctt
     3061 tcaacaaaac gaatcggaga atcaccgtaa tcttcttgtg taaatgtagt atgagaagta
     3121 ataaaccgtt caccataaac atcgccatcc atataagaag accgatcgaa cattttttca
     3181 tttaattcat tgatagcgtt caaatctgta cagccttgtt ctttgaatac ccaattgacc
     3241 atattaaaat caggatgatt caatggatag acttcgattg tttttccttt aacagtaaaa
     3301 gtcagatgat cgagaaattc tctgaatctt tgagctgcct cgattgaagc cccaatcaat
     3361 tgaccatatc cggtcacatt caatggcaat acacgatgtg ctgtccagac tgcggcggcg
     3421 gttgctccag cttttgatcc ttccaaaata taagctccta acatatctgg agcctttact
     3481 gatttttcaa acacatatgg cgcgaaataa gaaatgatat ttctcatatt tttatgtttg
     3541 attacgattc ctccagcagc ataagggaca tagcccatct tatgaggatc tatcgtgaca
     3601 gaatccgctt ctgaaatagc tctgaaccct tcatatacct ctttgtcaat cgttacgcag
     3661 tggtggaaaa catgatgttc ttcaaagaat tcagctaagg aagcatacgg cacaaattct
     3721 cctgcttcgt ttagaaacag tgaacgcgca tagccgccat atgcggcatc aacatgtaaa
     3781 tagaaataga tcccttcatc ttttaacctt tctcttaatt ggacgatttt atctacactg
     3841 tctacttgac cttcttctgt cgttcctacg acagcaacca cacctagaat tgggattttt
     3901 tgtccagcta attcacgaat tgttttttcc aaagcattaa tatccattcg ataatcctct
     3961 tgaactggga tagcaaccat ctgatctaag ccgactccgc aaatatctaa cgccttcatc
     4021 caagaatagt gttttgtttg agggaccagc catttcccta atctttggat attttttccg
     4081 ctacgggaag aagctgcttt gacctcatcc atttcttcgt ccgtaaagct ttcggtcatt
     4141 tccaaaattt cttcaacaga taaattcaaa agttcccact cagacatttt tttcactttt
     4201 tcaggcagca cttccttgac agctaaagga atcgatttga tgcaacgggc gtaccataat
     4261 ccttcaagat tagcaataga accatctgct gcaatgtgtc cccatccgtc tgtcatattg
     4321 aataatgaag caaaatcctg tcctacttct gcttccattt gcgaagttgc catagaagat
     4381 tccaatgcaa cattatttgg gttccacagc atcgcatagt tgtaagctaa taaagcaggc
     4441 atcaatgttt cagcattcat ttgtccccag taacggcctg ctgaatgcca tgggatcgat
     4501 cctgcgcgta atcgctgact gacttcatcc aagacctcta tcatatggtc gcgggtggct
     4561 agataatctg gagtgagctt atctccttcg ctaattgcag gcatatctga aggaatataa
     4621 ttttctctcc atccaagatg ttcatctacc atcttgttca acaacatttt ataaactggt
     4681 ccattttcag ctttgtcgcc tataaagacg gccttgatat ccatatcttt catttttctt
     4741 ccctccatat tttgttggtc aatgtcttgc agatacatgt gtttatctgt aacacctctt
     4801 cactatagcg ccaaaaatga gaattttttt tttataacat caaatccacg tagattttta
     4861 caaacaaaaa taaataaagt cggatttttc tgataattca acgcaaaaaa gcgaacaatt
     4921 tttcaataaa tcagtcttct aaaatagata acctaaaaaa agcgaacaaa caaataaaat
     4981 gactgtcctt cattcactaa acatattttt atttttttat attgttaaaa attcgcaatt
     5041 tattttttaa tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg
     5101 gcatttatat gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata
     5161 tttcccatat tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt
     5221 gagaaaaata ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc
     5281 aatctagaaa agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc
     5341 aataacataa aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt
     5401 tacactttat atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa
     5461 aattcgttgt ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt
     5521 caattaactg aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta
     5581 ttagcttatt ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa
     5641 atggaaagac gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg
     5701 gagctacctg aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat
     5761 agtggcagat tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt
     5821 ttcctacgcc agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa
     5881 aaacgtccta tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat
     5941 tataaaaaag aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc
     6001 cattcttatc agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat
     6061 actcccgaaa taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc
     6121 ggaaaccaac cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat
     6181 caattattca tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa
     6241 gaagtccaga caattttttc cagctggtcc agccagttac cttatgatct acggttgaat
     6301 cctaattgca tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa
     6361 catctgacta tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc
     6421 ttggaagctg taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct
     6481 gatataaaga agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat
     6541 acaccagatg cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga
     6601 gctatcattt cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct
     6661 cttatttgtt gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac
     6721 ctcacttatt ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg
     6781 ttcggataac taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg
     6841 attcgatgat cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca
     6901 tctttttaat agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata
     6961 aatatttcaa aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa
     7021 agatatcaag tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg
     7081 tcgtcatcct acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa
     7141 ataagaaatg actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt
     7201 cggcttgata actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat
     7261 ataacttgct agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt
     7321 cttgacatga gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat
     7381 accgatagaa caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga
     7441 gcgaaacata tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga
     7501 aagttacctt gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt
     7561 ctttttcact ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac
     7621 gtagcaagtt aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt
     7681 gctttttttg tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc
     7741 ttacctaccc tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt
     7801 cctttttctt ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata
     7861 gaaagagaaa acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt
     7921 ttaggtttgc ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat
     7981 gacagtcatt tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac
     8041 ggacatactt ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg
     8101 gtgccggtat tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag
     8161 gggatcagat caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg
     8221 acactgtagt ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata
     8281 ttgctcgagg cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa
     8341 gtcgaatacc ttctgttact gagccaggga tgactgctga agaagatacg agagattttg
     8401 atgcaaaaca ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg
     8461 atggcggaaa actgggattc aataataaag accgtattct tctgactaaa ctgattgcta
     8521 tgcctgattc agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg
     8581 atccacactt ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa
     8641 cacgtagttc tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc
     8701 aaatcgaaca tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc
     8761 ttcctttgat cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca
     8821 ctgactggga attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa
     8881 ttaccaatac actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta
     8941 ttttcactaa tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg
     9001 tagaaaatat ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct
     9061 acaacttggg aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct
     9121 ttaccctgac gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag
     9181 aaccaggaaa tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa
     9241 aagatgtcaa tatgtctatc gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa
     9301 atgaaacagt tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata
     9361 aacagtatat taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa
     9421 aagtagatcc tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa
     9481 taaataatat ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa
     9541 gcgatcgacc aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg
     9601 tcgagcagcc ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg
     9661 ctgctttcca ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca
     9721 aagatattcc tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg
     9781 aaaaagctgc cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt
     9841 tgatttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt
     9901 gtgaaaaagc tgccctgcat caagtaataa gaacacccaa gctaaaactg cttcttatgt
     9961 ttttgacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttaccaagag
    10021 gttatgaaaa agctgccctg catcaagtaa taagagcagc caagctaaaa cagcttctta
    10081 tgtttttgac ttggttagac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
    10141 gaggttatga aaaaggcgtt tagtttcgag tatgaagaaa aattttgtga aaatggttcc
    10201 tcacgttttt gcataaattt gacttaatac cgagaaactg atcccttctg tcataacctc
    10261 tttttattta tgtctgtttc tttttgaaaa caactatttt ttcaacgttt actctcttgc
    10321 caatccaaca tcatctaacg gatacaacta tttcaatgat taccattaca ctaaaaaatc
    10381 ggaaacacac ctctccgaac acaaatacat aagactatag aatgtttcat ttaagaaaaa
    10441 aagcgtttca ttatcaaaaa ggtaagatct cctttaaaat gaaattatct gtagtactaa
    10501 taatgcagac aattttatga atctgaaagg agcatctcca atgaaagcag tcacttggca
    10561 aggaaaacag aaaatggaaa tcagaaacgt agatgatcca aaaatattag aaccaacaga
    10621 tgcaatcatc cgtatcacag caacagccat ttgcggctcc gatctgcatc tttaccatca
    10681 tggtgaagca gttttggaaa aagggtatgt tgtaggacac gaaccaatgg gaatcgttga
    10741 agaagtggga tctcaagttc aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa
    10801 tattagttgt ggtcattgtc atttctgcct gaatcacatg gaaagccaat gtgatgaatc
    10861 aaatccagat cagttatatg gcgggctttt cggatttggc aagttgaacg gtaatcattg
    10921 gggtggtcaa gcggaatatc taagagttcc ttatgcagac tctacttcat ttgttgtacc
    10981 agataatgat cttcctgatg aaaaagtttt atttctatca gatattttgc ccacagctta
    11041 ttggagcgtt gaaaacgcag gagtaaaaaa aggcgataca gttgtgattc taggttctgg
    11101 tccagtcggt ctattcgctc aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc
    11161 agtagatcca gtacagcacc gtctagataa agcggctaat tacaatgggg ttgaaactta
    11221 tttcttagaa gacacggcac aagctggaac agaattgtat gaactggcta aaggtggcgc
    11281 agatgtcatt atcgattgtg taggaatgga cggattagag cctgtcaaag aaaaagcgaa
    11341 aaatttagta agcttacaaa gcggcacaat ctctccatta cagatggcat cgcaagctgt
    11401 caaaaaattt ggaacagtcc aagtaactgg tgtttatatg actccagctt cttcttaccc
    11461 gctgcaagaa ttttttatgc gggatatcgc tgtcaagcat ggacaggcac caattatcca
    11521 tctaatgcca aaaatttacg caatgattgc agaaggacta ttcgatccta cacaaatcat
    11581 tactcattcg ctgccactat cagaagcagc acaagcatat gagatctttg acaaaaaaga
    11641 agataaaaat ataaaagttg ttttgaaacc ttgaattcag taaaaaatac aggtgatttt
    11701 ctttatgccc aaaatcaaac gaaataccca tgagatcgaa ataggaaaag aagaagatat
    11761 cgaaatacga ggacgtcgct tccgcttcta tttccaaaat cgttacacat tgatctcact
    11821 ggctgtcgat ttattaaccg ggattttcta tatttttgga agccttgctt ccttaactaa
    11881 aatccccgat cgatatggca cctattttta tttggctggt gctattttct tgacgattcg
    11941 tcctattctc agaatcgtaa ggaacatttt tatttacgac gaaaaaaaag caatgaagag
    12001 aacaagcaaa aaaaaagagg catgagaatt tttcaaatct catgcctctt ttttcaccct
    12061 atcgtatcac tttgatacag acgagcggtc gtcccacttt cttccttgac cattgtcaaa
    12121 aatgtccact cgtatcgttc ataaaacgtg gtatgatctg taattaagta aagtttttcg
    12181 tacccaagtt gatgcatttc ttttctgatt tccgctaata tccgcttggc tatcccctgc
    12241 ttacgctttt ccggttcaac aaacagggca caaagatttg gtgtcagatc agggcggtta
    12301 tggaagtcgt tttcaatgac gcccgctcct ccgacgatgg tttctttttc atctaagacc
    12361 acataccatt gaggaatact ttccttatta gcgattccag tacgaatact ttcctcatac
    12421 acaacagcag gaactcccca ttttgatgaa aaccaattcg cgctttgttt tactaattct
    12481 gggtgttctc ttaatttgat ccagttatat gtaaccaatg tttaaacctc cgactaaaat
    12541 aaaacgttta caaaaattat acaagtcttt atgaaaatta gcaatgacaa caatagtgat
    12601 gtaaaaaaaa aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa
    12661 agcttctttt cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat
    12721 agaaagaacc aaattgacag cggctagtac accacctgca atactttttg ttgccaatac
    12781 accgccaata attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc
    12841 tgtagaagtc gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat
    12901 gaacatgatc gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa
    12961 gacacctggc gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac
    13021 taaataaata gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct
    13081 gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgc ccagctgcga ttgcattaac
    13141 gtttgcttca atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc
    13201 gtgtaaacca aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata
    13261 agagtttgca agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc
    13321 atcaacaccg aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat
    13381 tgctggtagt aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt
    13441 gatgatcaat ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac
    13501 aataatagca ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa
    13561 agagcctaaa gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg
    13621 ataagcaagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag
    13681 cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga
    13741 acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca
    13801 tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg
    13861 ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt
    13921 ttctttactg tagattacgc gttcaatcga cgatacagat caatgatctc ttttgctaaa
    13981 tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact
    14041 tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg
    14101 actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc
    14161 gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc
    14221 atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta
    14281 tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc
    14341 agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt
    14401 ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg
    14461 attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc
    14521 tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat
    14581 gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg tatataagca
    14641 agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat
    14701 accaagttgc atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat
    14761 tacctttttt cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt
    14821 ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
    14881 cataagatgt tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca
    14941 acctctcaat aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt
    15001 cacaacctct tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta
    15061 tttatttata gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc
    15121 acgaagcaga ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc
    15181 catatcgatt cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat
    15241 agccttcgca atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga
    15301 taaattcgat acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat
    15361 attctcttcc cataccgttt tctgttatca gccaagggat attatgataa ttttctttta
    15421 gatacatggc tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca
    15481 ttttcttccc aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt
    15541 cattccattg ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga
    15601 aatctactgt atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg
    15661 attgtttttg acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa
    15721 aaaatactcg attataaaac aaatcataca tataagcagc tttttggtct tctggagaat
    15781 tggatcgagc atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc
    15841 cgctgcggta aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca
    15901 tccacttttt tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt
    15961 ggggaacgat tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa
    16021 aggcaatttg agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat
    16081 atttttccaa aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt
    16141 ctgcttcgat caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta
    16201 tacagttctc ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca
    16261 gatggatgga tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta
    16321 cggctgggcc atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa
    16381 acgaatcctg tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag
    16441 caccaagaat aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc
    16501 ccatcatttt ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca
    16561 ataatacaaa caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca
    16621 ttgacgatcg gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa
    16681 gcggttgcaa aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct
    16741 aaaattggat tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc
    16801 ccaggagcaa gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat
    16861 actgcaatca gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa
    16921 aatggcatag taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca
    16981 gcaattgccg ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata
    17041 ccaatcaccc aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt
    17101 acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct
    17161 gtaagttctt tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg
    17221 aataaagcag aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt
    17281 agtttcttct gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt
    17341 cccaaaaata accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca
    17401 gtctgatttt cattcactaa ttcataaaca gttggattaa cagataaata agacatcact
    17461 gcgatgatac cagggaatac accatccact ttattcagct tggctaattc gatccctatc
    17521 agaaaaacag catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata
    17581 ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca
    17641 aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca
    17701 gcaaatgcgt tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc
    17761 cctaaaacat tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct
    17821 tctccttgac gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa
    17881 ctatttactc ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata
    17941 aaatacatca gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc
    18001 tccgatttat tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt
    18061 cgatggcctg aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat
    18121 cataattttt attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg
    18181 ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt
    18241 cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga
    18301 tttgattctt cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc
    18361 tcagttcaaa taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg
    18421 ggtcattggc aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa
    18481 ggatattcct ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta
    18541 aataatcgga caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct
    18601 ttaaatatct aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac
    18661 cacatacctc cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat
    18721 ctgatgattt tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga
    18781 tatttaccat aaaaaagact aggataaaat tcactcctag ccttcgatca tttttatttt
    18841 gaacgacaaa aaatatgatg ttcaataaca gacgatcttc attcgattct tgatgtcatc
    18901 ttttgttttt tttaatatat ggataaaaag tttgattaca tcgtttctat tctagaattt
    18961 ggtcgattct caattcttct tctggtgaga aatctaaatt atccatcatt cgaacacttt
    19021 cttgtaactg acttaaccga cttgctccga ccagtacact tgtaacagat ttttgtcgca
    19081 aattccatgc aagggccatt tgcgctaatg attgtcctcg tgatacggcg attgtttgca
    19141 gtgctttgat tttttccaga gttgatccaa cttgttcttc tgaaaggaat ggtatttcct
    19201 tacgatgtgc tctagaatct tcaggaatac catgtagata acgatttgtc agcaaccctt
    19261 gagcaagcgg actgaacgtg attgccccta gcccttcttc ttctaatact ggagatagat
    19321 cgtcttctag tagtgcacga gaaaacatat tgtagcgcat ctgatgaata atgaacggtg
    19381 cttcctttct ttttaaaatt tcagtcattt tttttgtatc ttctccatta tagttggata
    19441 taccaatata taatgctttt ccttgtctta caagttgcat caaagcttct gctgtttctt
    19501 ccaaaggtgt atcagggtct gggcgatgat gatagaagat gtcaacataa tctagcccca
    19561 ttctttggag actttggtca caacttgaaa tgatggattt acgagagccc cattccccat
    19621 atggcccttc ccacatatga taacctgctt tgctagaaat aatcagttca tcacggtagg
    19681 cttgaaaatc ttctctaaaa atacgcccaa agtttttctc tgcagctcca gcagggcctc
    19741 cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct cttaaaatct
    19801 ctctttgatt atggatagga tcataatcac caaaattttg ccataatccg agagataaaa
    19861 tcggtaattt caaacctgaa tgaccggatt tacgatattc cattttttca taacgtgcac
    19921 tatctgcaat atacaatttt tctcctcctc atcaaatctt tttaaaacgc tttctttttt
    19981 ctatttttag tttactacat tttaaagaaa aggagttaat caaaatgatt ttttcgtata
    20041 tttttttgcc atttgttttt tactcgttga tagatcagaa attgattacg tgcttgctgg
    20101 atttttttat aacgtttcgg ttgttttttg taaaattgct gatgatagtt ttctgctggc
    20161 caaaacgtac cggctggttc gatttttgtg acgattggtt ggtggaaagt tcctgaactg
    20221 tctagtttct gttttgattg ttccgctaat tctttttgtc gttcgccttg ataaaaaatc
    20281 actggacgat attgcttccc tctatcttga aattgtccaa atgtatctgt gggatcagta
    20341 atttgccaat agattgtcag caactcgcta taacttattt ctcttgtgtc ataaatgatc
    20401 tccacagctt ccacatgacc tgtgtaacca ccacttactt gatcataatt tggtttttca
    20461 acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc cttttttcga ttcaaacggc
    20521 tcgaccatac accagaaaca tccccctgca aatactgcac gttcttgata gtcagcatct
    20581 tgcatggcat gcttggaaaa gtcatatttt tgctccttat caaatccttc cgtaatcaat
    20641 aaataaaagt ccattacatc aggcgtcaga ttcattcgga tagctaatgg acgtagtgtt
    20701 gcttctagtt ttgataactg ttctttaacg gatatatttt ctcttatgtc attctttgtc
    20761 tgaatcaata cttttcgttc ccaatctcgt gtattcggat tcaaaatcag attataaagt
    20821 gtttgaatga atatttgttc ctcatccata gattctcctc ctttccgaag aagacaagct
    20881 agtgacttta gctgtaattt atcataaagt gaggaaataa gcgattactt catcccccta
    20941 cagatttttc gtataaaaaa gagagaatcc ttatcgacag attctccttt ttaatcaatt
    21001 tattttataa atgaaaagtt aatttacctt ttatggaaaa ctattttaaa aaataaacat
    21061 aaatatgcag ttaatgtaac aagataagaa ataatagtcg ttactgtata acttgtgctt
    21121 actttaatat cagcaactcc tttgactccg cttatttgta ccaatccatt agcattcacc
    21181 tgctccttta caggttgatg atttatttct acattcgttc ctaaataagc atatacagga
    21241 agtgtgatta cttggctctt ctcattagtt actctatata tgatagaact accagttgtt
    21301 ttataaggaa ggtttaccca ttgactgcca atctttgctt cgtgttttat tatatgctcc
    21361 ttattctcca atgaagcgat aggagaataa tcatataaaa aataatccat tcctttagtg
    21421 gtatacgcct ctactgtttc tttgtttaca acaatatgtc ccggctgaga aattattttt
    21481 tgattgaaat tttttttact agaaacagtt aaagtgatta ataacaaaac agtcataaat
    21541 aaataaaata gttctcttgt cttattgtat cttctatttt tgaaaaatat catcgttaat
    21601 atcagagaag agaatattat ttgcaagctt aagatccgcc aaggaaattg taacaatttg
    21661 gctggcgtat tttgcagaag ataccatggc accaaactag ttgtagctag ccaagtcaaa
    21721 cacgtaccat aataaatctt tttttctatg tttgttaatt ttttaaataa aatcagggga
    21781 atacataaag ataacagacc aactagtccc attgagtaag tagttaagtc gttgttcata
    21841 gcttcagtca gactatcgaa cacattagat gcatgagcat ataaatttgt cacgctaggt
    21901 ttgttgattt tttggtagag catttgttca aacatcggat accaaaaagc actcgtcaga
    21961 accaatgtca ttcctgccgc actaaataat tttttaaata tagcaataaa atctgcattc
    22021 ttgaaacctt tagaaaccaa acgaataaaa ataaaaatga taattaatgc tgtactcatt
    22081 aatgttgata gtacatggct gtacactagc aaactcatac tcaatgctag ctttacagac
    22141 ggatactttt cttcttttcc agagatgatt tgatgagcat aaaataatac agggataaca
    22201 agtgttattg cgatagcttc ggcaattgcg gaacgatagt agatgtctac tgtccgataa
    22261 gcagagaaaa tatagagaca actaaataaa aatgccgctt ttttttgttt taaaaaccgc
    22321 tcgccataat gataagctac gagtgttgta cacaagttca ataaccaaac gtaaataata
    22381 tagcttacaa tcaaattttt tgtcatccaa tataacatta cagctggaaa aaaagtcaga
    22441 aagggataaa aataatttac accatatcca gtataattga atgtctcata attaatcgga
    22501 ctggtaaaaa tagtcgataa ccccttgatt cgagataagt gaaaagaaag atctccagtg
    22561 gtataaatac tctgtgtatt tttgaaaaag atgaaagaat atatactact aactataatt
    22621 aatccactca atattactac ttttttattt attttcattc ttcttgttta tctccttttt
    22681 agtcacatat cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac ttgaatacac
    22741 cactaaacaa taagtcagaa tactgatcca taaagccatg agagcagttg gagtatattt
    22801 tgttgtaatt tctatcttat tttctccagc atttacatcc acattcacta agccatgttt
    22861 tttataagag tcatttacga ctttaccgtt tacttttacc tgtgtaccta aataagcata
    22921 tactggtact gtcacttttt gctttttgtt gcttgccact gtgtaagtaa tgttattatc
    22981 tgcagcttta tacttccctt tttcccacga ttctcctact tttacttcat gtttttctaa
    23041 gtgaggcctt tcttttatcg cttctgttgg ggcgtaatcg tataatcctc ccatatctga
    23101 tgttgtataa cgtgggagtg tttcttcatt gataacaata tgaccgtgtt ttgattggat
    23161 aacttttgcg taattttctt ttgctgctat agacgtgacg agtagaaaca ccacagtagc
    23221 gccaatgatt aaccatgaat attttttatt catagtcttt ttcataaaaa caagcgttat
    23281 gatcattgaa gaaaaaaaga tttggatacc taagatacgc caaggaaact ggagcaagct
    23341 tatcggtgtt ttttgaaaca aaaaccatgg aaataaatta gttacgcata cccatgtaga
    23401 aactacgcca acatagatga ttttttcttt tttctccatt tctttcagta tgaataaagg
    23461 aaaaatcaac gcaaccaatc ctaataaccc catagtgtat gttgtaatat cgttatttaa
    23521 cgcttgaatg atactatctg aaatagaaag tgcttctagc tgcaaatctg ttttgaaggg
    23581 actgtgtatt tcttgataat tcatctgctg caacatagga taccaaaaat agctagttaa
    23641 tgcacaagtc aaaaaaacag cctttactaa attcacgacc acaaatttta tatcgttttt
    23701 tatttgcttg cttaaaaaca aacgaactat aatcatacaa gcaatcaatg cactacacaa
    23761 tagtgtagtt aagacatgag tatacacaag tagacttata ctgacagata aagcgatgac
    23821 ttttttttct ctattgaata tcaatccata agcataatat aaaacagctg ggataatcgt
    23881 aagagcgatt gcttctgcaa ttgcggatcg ataatagata tcaactgttc gataagttgc
    23941 aaaagtatat aaacaactaa ataaaaaagc agcatctatc tttttaaaaa acttttctcc
    24001 ataataaaaa ct
//
LOCUS       DACEJR010000043        23650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEJR010000043 DACEJR010000000
VERSION     DACEJR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.043"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            248..523
                     /locus_tag="IW651_001775"
     CDS             248..523
                     /locus_tag="IW651_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814498.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            536..2614
                     /locus_tag="IW651_001776"
     CDS             536..2614
                     /locus_tag="IW651_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0814499.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2774..4660
                     /locus_tag="IW651_001777"
     CDS             2774..4660
                     /locus_tag="IW651_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0814500.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4672..5619
                     /locus_tag="IW651_001778"
     CDS             4672..5619
                     /locus_tag="IW651_001778"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0814501.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5639..6166
                     /locus_tag="IW651_001779"
     CDS             5639..6166
                     /locus_tag="IW651_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0814502.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6229..6882)
                     /locus_tag="IW651_001780"
     CDS             complement(6229..6882)
                     /locus_tag="IW651_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0814503.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7014..7856
                     /locus_tag="IW651_001781"
     CDS             7014..7856
                     /locus_tag="IW651_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0814504.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8014..8910
                     /locus_tag="IW651_001782"
     CDS             8014..8910
                     /locus_tag="IW651_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0814505.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8913..9626
                     /locus_tag="IW651_001783"
     CDS             8913..9626
                     /locus_tag="IW651_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0814506.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9646..10164
                     /gene="msrA"
                     /locus_tag="IW651_001784"
     CDS             9646..10164
                     /gene="msrA"
                     /locus_tag="IW651_001784"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0814507.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10161..10388
                     /locus_tag="IW651_001785"
     CDS             10161..10388
                     /locus_tag="IW651_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0814508.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10540..11091
                     /gene="lepB"
                     /locus_tag="IW651_001786"
     CDS             10540..11091
                     /gene="lepB"
                     /locus_tag="IW651_001786"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0814509.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11174..11716)
                     /locus_tag="IW651_001787"
     CDS             complement(11174..11716)
                     /locus_tag="IW651_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0814510.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11953..12351)
                     /locus_tag="IW651_001788"
     CDS             complement(11953..12351)
                     /locus_tag="IW651_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0814511.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12611..13471
                     /gene="ylqF"
                     /locus_tag="IW651_001789"
     CDS             12611..13471
                     /gene="ylqF"
                     /locus_tag="IW651_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0814512.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13464..14231
                     /locus_tag="IW651_001790"
     CDS             13464..14231
                     /locus_tag="IW651_001790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0814513.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14287..15144
                     /gene="dprA"
                     /locus_tag="IW651_001791"
     CDS             14287..15144
                     /gene="dprA"
                     /locus_tag="IW651_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0814514.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15266..17344
                     /gene="topA"
                     /locus_tag="IW651_001792"
     CDS             15266..17344
                     /gene="topA"
                     /locus_tag="IW651_001792"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0814515.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17313..18689
                     /gene="trmFO"
                     /locus_tag="IW651_001793"
     CDS             17313..18689
                     /gene="trmFO"
                     /locus_tag="IW651_001793"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0814516.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18887..19792
                     /gene="xerC"
                     /locus_tag="IW651_001794"
     CDS             18887..19792
                     /gene="xerC"
                     /locus_tag="IW651_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0814517.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19829..20377
                     /gene="hslV"
                     /locus_tag="IW651_001795"
     CDS             19829..20377
                     /gene="hslV"
                     /locus_tag="IW651_001795"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0814518.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20391..21791
                     /gene="hslU"
                     /locus_tag="IW651_001796"
     CDS             20391..21791
                     /gene="hslU"
                     /locus_tag="IW651_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0814519.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21809..22600
                     /gene="codY"
                     /locus_tag="IW651_001797"
     CDS             21809..22600
                     /gene="codY"
                     /locus_tag="IW651_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0814520.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22682..23557
                     /locus_tag="IW651_001798"
     CDS             22682..23557
                     /locus_tag="IW651_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0814521.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4078 c         4981 g         6399 t
ORIGIN      
        1 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
       61 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
      121 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
      181 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
      241 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
      301 cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
      361 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
      421 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
      481 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
      541 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
      601 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
      661 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
      721 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
      781 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
      841 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
      901 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
      961 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     1021 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     1081 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     1141 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     1201 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     1261 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     1321 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     1381 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     1441 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     1501 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     1561 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     1621 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     1681 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     1741 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     1801 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     1861 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     1921 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     1981 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     2041 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     2101 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     2161 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     2221 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     2281 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     2341 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     2401 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     2461 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     2521 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     2581 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     2641 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     2701 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     2761 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     2821 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     2881 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     2941 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     3001 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     3061 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     3121 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     3181 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     3241 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     3301 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     3361 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     3421 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     3481 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     3541 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     3601 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     3661 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     3721 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     3781 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     3841 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     3901 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     3961 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     4021 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     4081 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     4141 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     4201 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     4261 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     4321 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     4381 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     4441 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     4501 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     4561 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     4621 ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag gatggaacaa
     4681 gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca
     4741 ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt
     4801 cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt
     4861 ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa
     4921 tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt
     4981 ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt
     5041 ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac
     5101 atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc
     5161 aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca
     5221 tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt
     5281 tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta
     5341 ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca
     5401 ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat
     5461 atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg
     5521 aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat
     5581 gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat
     5641 gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc
     5701 ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt
     5761 gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat
     5821 tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga
     5881 agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc
     5941 tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt
     6001 attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac
     6061 tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg
     6121 taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata
     6181 gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa
     6241 taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg
     6301 catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc
     6361 tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac
     6421 gacacagagc caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg
     6481 tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac
     6541 aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc
     6601 tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt
     6661 aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata
     6721 gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac
     6781 cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc
     6841 atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact
     6901 aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac
     6961 atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa
     7021 atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag
     7081 gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc
     7141 aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa
     7201 gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg
     7261 aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag
     7321 ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat
     7381 tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa
     7441 tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac
     7501 tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt
     7561 tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc
     7621 gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc
     7681 caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca
     7741 aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg
     7801 ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga
     7861 aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat
     7921 taggaaaata attgttttct tttttcataa agactacctt tttgataaaa tttttgataa
     7981 cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg acatttgatc
     8041 ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc tgcttctata
     8101 ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc aaataaaaaa
     8161 gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg agatcaaacc
     8221 aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta taatttgacc
     8281 gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga
     8341 gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat cacactgact
     8401 gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt aacggtcaat
     8461 tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt ggaagaattt
     8521 cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc attttatttg
     8581 aactttcctg agatcactga catgcagaca attgttaaca attggaacaa aggaacagaa
     8641 acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt gctttatcaa
     8701 ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac tagcacgaca
     8761 gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt ccatccaaat
     8821 aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga gacacaaaac
     8881 caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac gtatcttcaa
     8941 acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt ggggtagtac
     9001 ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca gaactcccag
     9061 aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat tcaaataaag
     9121 aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat agtgatattg
     9181 aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa gtactaggtt
     9241 ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga aatgtccaac
     9301 taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat gtgatgaata
     9361 tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat gatctgacta
     9421 tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa gcagataaaa
     9481 tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat acctctgatg
     9541 cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc aagacaaaag
     9601 aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga acgagcaata
     9661 tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc tggtatccat
     9721 acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga acaagtaaaa
     9781 agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac attgatttca
     9841 tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc tttcggacaa
     9901 ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga agaacagcgg
     9961 atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga acagccaatc
    10021 gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca tcaagagtat
    10081 tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc ctttatcgaa
    10141 gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg acactgcgag
    10201 gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa gacatccaat
    10261 ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg aatgtcgact
    10321 atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa aataatcatg
    10381 acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca atagcctaat
    10441 tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt ttcttataat
    10501 aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa acaacgctat
    10561 attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct tgcgttcatg
    10621 ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga aaacgtgttg
    10681 aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt tgatattgtt
    10741 gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct tccaggagaa
    10801 aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa ggaaccttat
    10861 ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga ctttacacta
    10921 caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact tggagataat
    10981 cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga tattctaggt
    11041 aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg agaaacaata
    11101 aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca gtttactacc
    11161 ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa gttttgataa
    11221 atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg aatctaccta
    11281 gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa ttgataaaaa
    11341 ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg tttatctttt
    11401 tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata agaaaagcag
    11461 cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac atttcaagca
    11521 aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa aatccgaatg
    11581 tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca agggctaata
    11641 aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa acgtcttttg
    11701 caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg ataccataaa
    11761 atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa caattaaaaa
    11821 aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat aaaaaaaata
    11881 gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa aaacgtcaga
    11941 ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt ctagatcttg
    12001 gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt ggaaagtacc
    12061 accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt caacactaaa
    12121 cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt ttataaattt
    12181 tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata ccattgaacc
    12241 agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat aaaaagcaac
    12301 ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca tttgtttcgt
    12361 ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca aaaatttaga
    12421 ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga tgtgatagaa
    12481 tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat aaaaaagtga
    12541 atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta tgaaaggaat
    12601 ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc gacgagaagt
    12661 ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac gattaccttt
    12721 atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc ttgttttatt
    12781 gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat attttcgtaa
    12841 acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta aaggtctagt
    12901 acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc gggggatcaa
    12961 gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat ctacgttgat
    13021 gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg taacaaaagg
    13081 tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag ggattttatg
    13141 gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg cgatcaaaga
    13201 ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg cccgatatta
    13261 ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa aattggcacc
    13321 ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg atcgtggcag
    13381 cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac tggatcgttg
    13441 cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa gaacgattga
    13501 aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat ccacgcaaag
    13561 gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca ttaattaagg
    13621 aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt caccaatgga
    13681 ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg gcagctgcag
    13741 ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa ttatcagaga
    13801 aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc gggatcggga
    13861 tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa ttagcgatga
    13921 tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat gcgatgacct
    13981 tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct gtgtcgattg
    14041 ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa tatgacgtgc
    14101 tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa cacttagaag
    14161 gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct gtaaaaaaat
    14221 atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac gaaaggcggt
    14281 tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa aggaatcagt
    14341 agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa agaaatcgga
    14401 tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt tcgcaaaagt
    14461 tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt tatcacttgc
    14521 tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat tgtactgttt
    14581 tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg tggaagacac
    14641 acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat agaagctgga
    14701 tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca agcagcaatc
    14761 tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt ctatcctgct
    14821 gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag tgaatatcct
    14881 cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat cgcagctttg
    14941 tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat aacagcacag
    15001 caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt aacagaacat
    15061 tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc gggtgaagat
    15121 atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt gacaaacgtt
    15181 ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag taggaacacg
    15241 acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg tagaatcacc
    15301 tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa tggccagtgt
    15361 tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa acaattatga
    15421 accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga aagccgcagc
    15481 aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag aagcgattgc
    15541 ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg tggtcttcaa
    15601 tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga tcgatgtaga
    15661 tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt attcgatcag
    15721 tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc aatctgtcgc
    15781 tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag aagaatattg
    15841 gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt tctggggaat
    15901 cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca ctgctagaat
    15961 tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa gaaaccctgc
    16021 gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa actttcgaac
    16081 aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg gtaaacaagg
    16141 aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg atacagcaaa
    16201 agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta gacatgatca
    16261 ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc gtccaacgag
    16321 tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat tgaaacttta
    16381 tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt tagatacaat
    16441 gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa aaatcaaatt
    16501 caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag agaaagaaaa
    16561 tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg aaccaaaaca
    16621 acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg cactagaaga
    16681 aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc aaagacgcta
    16741 ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa ttgtcaattc
    16801 attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag aaatggaagg
    16861 agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg atcgtttcta
    16921 taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa ttcaaatcaa
    16981 agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga taaaattagg
    17041 gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata cgaaaccaat
    17101 cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca ttgaacgaaa
    17161 atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg aattcacttc
    17221 ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg tcgagaagaa
    17281 agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag acatccaaaa
    17341 ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca gggcttgccg
    17401 gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta tatgaaatgc
    17461 gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta gtatgttcca
    17521 attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa gaaatgcgcc
    17581 gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct ggtggtgcct
    17641 tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa gagcatccat
    17701 tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc gtagtagcaa
    17761 caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat ggttcagatg
    17821 gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc gatatggata
    17881 aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac tgtccgatga
    17941 cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg gcagaactta
    18001 aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta atggcacagc
    18061 gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa gatcctaaaa
    18121 ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca gcttctttat
    18181 acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt gttttccgga
    18241 tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac cgaaacagct
    18301 ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc gaagatctgt
    18361 tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg agcggactac
    18421 ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag tttccacaag
    18481 aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa catttccaac
    18541 cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga gacaaaaaag
    18601 caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata aaaacagaat
    18661 tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg agacggatag
    18721 tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg aggaattgcg
    18781 ccaattgtga acaaacttag aaatattggt taaattctga caatttattt gtctttttat
    18841 aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga cagaagccac
    18901 gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag aaaaaacgaa
    18961 agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat ctggagacgc
    19021 tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat tagctgatca
    19081 tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac gttcgtttta
    19141 tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg ttcatctcaa
    19201 aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag cactttttga
    19261 cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg aagtccttta
    19321 cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca ttgattttga
    19381 ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc ctctaggttc
    19441 ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat tgatgacaaa
    19501 atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa tcacgcctac
    19561 tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata gtgatatcca
    19621 tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg cagatttgcg
    19681 gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct atgcccatgt
    19741 aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt aaaaataagg
    19801 aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt caaccactat
    19861 ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag taacaatggg
    19921 tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca atggagaagt
    19981 tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa aatttgaagg
    20041 caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag ctcaagaatg
    20101 gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga atgataaaga
    20161 aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta tcttagctat
    20221 cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact atgctcaaaa
    20281 cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag atatctgtat
    20341 ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat atgtatgaat
    20401 taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta ggacagcacg
    20461 cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg cagttagatg
    20521 aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct acaggggtcg
    20581 gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt gtcaaagtcg
    20641 aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca atggttcggg
    20701 atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt gtatacacaa
    20761 gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt atcaaaaaag
    20821 aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat gctagtcagc
    20881 aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat cgccgtacag
    20941 tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt gaagttgaag
    21001 accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg atcgacctaa
    21061 atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta acagtcaaag
    21121 aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat gctgatatcc
    21181 atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat gaaatcgata
    21241 agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc caaagagaca
    21301 tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc caaacagatc
    21361 acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat ctaattccag
    21421 aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct gaagattttg
    21481 tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg ttgatcggta
    21541 cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct caaatcgctt
    21601 tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc attttggaaa
    21661 aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa ataacgatca
    21721 cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat ttaagtcgct
    21781 atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa cccgcaagat
    21841 aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc cttacgataa
    21901 aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag gaagtgacgg
    21961 agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca aaacaatgtt
    22021 tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga tgaaaactga
    22081 agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa gagagaaata
    22141 tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt tgggtacgat
    22201 tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag cagaatatgg
    22261 agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg aagcagatgt
    22321 acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg aattgaaggc
    22381 tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg cttctaatat
    22441 tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa aattggaatc
    22501 agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa aagtattgaa
    22561 ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg actggggcag
    22621 atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg gaggaatggc
    22681 aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac acggtgcgga
    22741 attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag gtgatccagc
    22801 gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa aagatgatca
    22861 gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc gggatatgga
    22921 ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga gtacgccgga
    22981 gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat taggaggaga
    23041 cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt tcttttctat
    23101 cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg aagatttcta
    23161 tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc cattcatcga
    23221 tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc gtgaactttt
    23281 tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca ttcgaagtga
    23341 aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag ggatctggtc
    23401 tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa tcgctgacac
    23461 ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag gtgcccatga
    23521 aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg agaagttagc
    23581 agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata agggagtgtg
    23641 acatcgattt
//
LOCUS       DACEJR010000044        23383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEJR010000044 DACEJR010000000
VERSION     DACEJR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.044"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1438)
                     /locus_tag="IW651_001799"
     CDS             complement(146..1438)
                     /locus_tag="IW651_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0814522.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1435..2058)
                     /locus_tag="IW651_001800"
     CDS             complement(1435..2058)
                     /locus_tag="IW651_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814523.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2061..2897)
                     /locus_tag="IW651_001801"
     CDS             complement(2061..2897)
                     /locus_tag="IW651_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0814524.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2914..3801)
                     /locus_tag="IW651_001802"
     CDS             complement(2914..3801)
                     /locus_tag="IW651_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0814525.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3817..5100)
                     /locus_tag="IW651_001803"
     CDS             complement(3817..5100)
                     /locus_tag="IW651_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0814526.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5783..6466)
                     /locus_tag="IW651_001804"
     CDS             complement(5783..6466)
                     /locus_tag="IW651_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0814527.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6484..7101)
                     /locus_tag="IW651_001805"
     CDS             complement(6484..7101)
                     /locus_tag="IW651_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0814528.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7111..7935)
                     /locus_tag="IW651_001806"
     CDS             complement(7111..7935)
                     /locus_tag="IW651_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0814529.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7928..8488)
                     /locus_tag="IW651_001807"
     CDS             complement(7928..8488)
                     /locus_tag="IW651_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814530.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8662..9618
                     /locus_tag="IW651_001808"
     CDS             8662..9618
                     /locus_tag="IW651_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0814531.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9923..10879)
                     /locus_tag="IW651_001809"
     CDS             complement(9923..10879)
                     /locus_tag="IW651_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0814532.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            10983..11585
                     /locus_tag="IW651_001810"
     CDS             10983..11585
                     /locus_tag="IW651_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0814533.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11673..13172)
                     /locus_tag="IW651_001811"
     CDS             complement(11673..13172)
                     /locus_tag="IW651_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0814534.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13188..13712)
                     /locus_tag="IW651_001812"
     CDS             complement(13188..13712)
                     /locus_tag="IW651_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814535.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14010..14759
                     /locus_tag="IW651_001813"
     CDS             14010..14759
                     /locus_tag="IW651_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0814536.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14750..15535
                     /locus_tag="IW651_001814"
     CDS             14750..15535
                     /locus_tag="IW651_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0814537.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15504..16220
                     /locus_tag="IW651_001815"
     CDS             15504..16220
                     /locus_tag="IW651_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0814538.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16322..17341)
                     /locus_tag="IW651_001816"
     CDS             complement(16322..17341)
                     /locus_tag="IW651_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0814539.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17360..18418)
                     /locus_tag="IW651_001817"
     CDS             complement(17360..18418)
                     /locus_tag="IW651_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0814540.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(18639..19787)
                     /gene="nagA"
                     /locus_tag="IW651_001818"
     CDS             complement(18639..19787)
                     /gene="nagA"
                     /locus_tag="IW651_001818"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0814541.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(19863..20699)
                     /locus_tag="IW651_001819"
     CDS             complement(19863..20699)
                     /locus_tag="IW651_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0814542.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(20883..22100)
                     /locus_tag="IW651_001820"
     CDS             complement(20883..22100)
                     /locus_tag="IW651_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0814543.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            22483..22566
                     /locus_tag="IW651_001821"
     tRNA            22483..22566
                     /locus_tag="IW651_001821"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22517..22519,aa:Leu,seq:gag)
     gene            complement(22806..>23383)
                     /locus_tag="IW651_001822"
     CDS             complement(22806..>23383)
                     /locus_tag="IW651_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814544.1"
                     /translation="NILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTM
                     GQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIY
                     LSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKEL
                     MKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         7036 a         4913 c         4032 g         7402 t
ORIGIN      
        1 taaaaaagca gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc
       61 cgaataaata gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg
      121 ggaagtaatt tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta
      181 ctgcttttcc tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac
      241 catctacgtg agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat
      301 catctaataa acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct
      361 catatggctc accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat
      421 cctttttatc tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc
      481 ctttgctgcc attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat
      541 acgtcaaaat cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga
      601 tatcatcgga aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat
      661 acatttctcg caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta
      721 gttcgcttcc atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca
      781 tcccataaaa gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa
      841 aattcactag atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct
      901 tatttcgatg ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga
      961 agtggataga aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt
     1021 gatacggtgc ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga
     1081 ccgttattat ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat
     1141 catatccttt tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa
     1201 taatgacttc agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg
     1261 tctttacttt tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg
     1321 tttgagatac atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg
     1381 cctcataaaa aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt
     1441 aaagtcattc tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag
     1501 taatgcagtg agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa
     1561 ataccacaga aaccccttga cgttttttga caacagccag atattccttt tgatcaaata
     1621 aacaagacta tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg
     1681 ggatacaaaa agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc
     1741 cactaataaa taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat
     1801 gaaacacatc cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg
     1861 gacagataga taataagaag gatacatcat caaaaaataa atcagataga caacacgagc
     1921 agacagcact aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact
     1981 gataagactg gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc
     2041 ttctattggc tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga
     2101 tatttattga agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca
     2161 aacatactcg cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat
     2221 accgtgtaag atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc
     2281 caataaaact ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt
     2341 ttggtaaaga cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg
     2401 gggatgctga ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt
     2461 ggtaaaatca gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc
     2521 ggaatcaaaa tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat
     2581 ctttttcctt tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact
     2641 gtccctatcg tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa
     2701 attccagcaa accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc
     2761 aaattcagcg aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat
     2821 aagaccggat aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc
     2881 gttactgtat tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga
     2941 tttgaaaatc agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact
     3001 agcatacccc atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt
     3061 cgtggcatca ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc
     3121 cgcgattgtc tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat
     3181 cttgtaaaac gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc
     3241 tgggatctct ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga
     3301 catcaaaaca agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc
     3361 gcctaatcgt tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac
     3421 tagagcgata gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa
     3481 agtcgttcct ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt
     3541 cacaggtaca gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc
     3601 atcactcagc atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg
     3661 ataatcggtg aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa
     3721 tcctaggatg aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg
     3781 ttttagtttt tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa
     3841 ctcataaaga ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac
     3901 taaaagattg tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc
     3961 gccgttgtcc atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc
     4021 atctgcacga gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt
     4081 taaaatttca ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc
     4141 tttatgggct gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga
     4201 ttctccatct gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc
     4261 ccctgacaat atttctgtca actggttact tgatagaaac accattgccg cttctctatc
     4321 cctaaccgcc tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg
     4381 ggcccctttg tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt
     4441 ttcaaatccc aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac
     4501 ttgtggtatt tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat
     4561 tcctaattct tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga
     4621 agctggttca gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga
     4681 attcaccccg ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga
     4741 ttcatctgca ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat
     4801 ctgattgatg tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga
     4861 tgctgctagt gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat
     4921 cgtcacatgg ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg
     4981 gacttgcgaa ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca
     5041 agccgtcaag aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat
     5101 tctctgcacc tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata
     5161 tttattaaac gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct
     5221 aggatgaaca actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc
     5281 taaaaacact tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag
     5341 acaactgccc aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc
     5401 ctaaaaaaca gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga
     5461 tgcacaagca ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg
     5521 acaatttagc tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc
     5581 tatagaaagc gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa
     5641 ggttttaact cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt
     5701 gaattctgaa caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc
     5761 ctcgtctttt gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt
     5821 ccattttacg actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc
     5881 ctgcttgaat ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa
     5941 gttcaggata ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga
     6001 tattgataat cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta
     6061 ccgtactgat aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg
     6121 taggaccgtt caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg
     6181 aagaacgagc accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg
     6241 cctcaataat gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg
     6301 cattcgcatt tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca
     6361 attttccaaa tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat
     6421 cttttctcaa ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct
     6481 ttgctatctc cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg
     6541 cgcataaccg gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata
     6601 gttggtggcc gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa
     6661 cagttgtgta tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg
     6721 gatagttatc cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc
     6781 cccataagcc ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca
     6841 atttgattct aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg
     6901 tagttcttgg gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt
     6961 ttcttgctga ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa
     7021 gagtatcact gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt
     7081 tttccacttt tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc
     7141 ttctagcgtc aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa
     7201 gtgccaaggt tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat
     7261 aaatccatac tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact
     7321 tgcatccaat acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc
     7381 cagacctgtg tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt
     7441 cgcctcctct tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac
     7501 ttgttgttgg gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc
     7561 ttcagcagcc ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc
     7621 gttacttaat gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg
     7681 accaaccaaa actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg
     7741 atcttttgtt tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact
     7801 agcagttgtt ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc
     7861 atcatcgcta aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc
     7921 taaaaattta ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat
     7981 gaacgtaagt ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt
     8041 cgatctgttt ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt
     8101 catcatattg aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca
     8161 aagggatcag ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt
     8221 tgatttgctt gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac
     8281 gcaaaatctt ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca
     8341 tcgcttcaga tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga
     8401 tcagcaagta ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca
     8461 taataagatc aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa
     8521 gttttgatca aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc
     8581 tctcctcttt attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca
     8641 actatgaagg agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc
     8701 atcgcacatg agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct
     8761 tccagaactt cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac
     8821 ggaagctatc aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca
     8881 aatagacagc atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt
     8941 gaaaaagcca tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa
     9001 aagaatttga tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta
     9061 cgctcaataa tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc
     9121 agttacaaag aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct
     9181 ctgttggata tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct
     9241 gaagtagttg cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact
     9301 atcttgcgta acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg
     9361 ccaaaagtcg gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt
     9421 gctgatcgtg cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat
     9481 acatctgaag caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg
     9541 cccaatcgct ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt
     9601 ctatggaaaa atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa
     9661 gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga
     9721 ggaaagcttt tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc
     9781 acaaggagca atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     9841 aggagttgac aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga
     9901 atattagctg aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga
     9961 tttcaataaa acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt
    10021 cagggcgact agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg
    10081 ttaaaatttg tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac
    10141 catagatatg ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt
    10201 ctttaatcac attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct
    10261 gtctgagcca gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg
    10321 tcagtaaatg ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg
    10381 ccatttgtct caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct
    10441 gcttcgtacg atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca
    10501 ttaatcccgc ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag
    10561 tgaccaaaac cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga
    10621 tgaacaggaa taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga
    10681 acagtgctgt tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg
    10741 tagagcgctt ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca
    10801 acagcgataa gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg
    10861 ttcgcattcc tatacgcatt tacttccccc ttttttattt taattatact tgaataatga
    10921 aaaactagct atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct
    10981 ctatgcaatt tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt
    11041 ttttatgtat cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat
    11101 ctgctgtttt ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga
    11161 tcatcggcaa tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt
    11221 ttcacaatga ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg
    11281 tcgtgtcaga tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc
    11341 tgattgcgtt gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag
    11401 atacttttat agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa
    11461 cagaaaagaa ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc
    11521 taagaacaaa tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg
    11581 actaattttc ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct
    11641 ccatttttga ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct
    11701 gcctcttttg gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc
    11761 tctacgattt ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa
    11821 agcaaatttg tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt
    11881 cctgtatcga caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc
    11941 aattgcggta gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag
    12001 gcattggcga tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg
    12061 gcttctgctt gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca
    12121 cggaatcgtt ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt
    12181 tctttagcgt accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca
    12241 cgacgtgtga tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc
    12301 tgcatttcct gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg
    12361 tctgcttttg ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt
    12421 tcttttaatg attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt
    12481 tctgcttgag cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca
    12541 tctcttttga cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct
    12601 ctgacctctt tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa
    12661 gcgacttctt gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc
    12721 atagaaccaa gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt
    12781 tcttctcttg tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa
    12841 ccgattttga tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact
    12901 tcaggggtgg agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa
    12961 actggcaaga cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca
    13021 atgtgcacat ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt
    13081 gctgtctggt acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt
    13141 gcgatcgcga taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc
    13201 atgctttcat aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc
    13261 gttccccgct cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa
    13321 ccggtaacac ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca
    13381 acagctactt gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc
    13441 atcggcatca atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta
    13501 gcaataaata aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag
    13561 cttgtgaaag ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat
    13621 acatctccaa aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa
    13681 taaatcgtct caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct
    13741 atcataccaa tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta
    13801 taggaaggtt gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt
    13861 cacaataaat tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc
    13921 tccagaacac gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg
    13981 ccatattgat caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct
    14041 ctagcattgc taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg
    14101 acccgacaca tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt
    14161 ttatcgttgt catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg
    14221 gtcttccttt ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct
    14281 ctgaaaaaga aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc
    14341 aagagattat tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca
    14401 aagatggaca aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag
    14461 gcgccgtttt tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa
    14521 tagcagaaca gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag
    14581 ccatctcttc tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc
    14641 aaacagagca gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt
    14701 tttgtttaaa gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa
    14761 tgggatagaa actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc
    14821 agtcgcaact ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac
    14881 gaaacaatat ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt
    14941 catttctggt ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct
    15001 aaatcaaaat tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat
    15061 tgccggtttt ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt
    15121 agaaagcgat tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca
    15181 agtgttaggg aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa
    15241 cggacaggtc cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt
    15301 ctgcgatgac ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt
    15361 ttcgacgaag ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg
    15421 ccacggtgga tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga
    15481 attaccaaaa aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa
    15541 tggttgcaga attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa
    15601 gtttcagaaa tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc
    15661 tgcttgatgt atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc
    15721 aaggaaatga tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga
    15781 atgtgatccg ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc
    15841 agctggcgat cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg
    15901 atcagaaatt gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat
    15961 tcatcgattt gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc
    16021 taaaagaaga tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta
    16081 tatccgtcaa gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca
    16141 aatatttgga aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa
    16201 agctcacaga ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag
    16261 aagtaacaac tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt
    16321 cttatcttaa ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta
    16381 ttatttcacg aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat
    16441 ttgaacctac atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag
    16501 cagggtctgg aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg
    16561 caaaaacatc ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt
    16621 cattatcttg aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag
    16681 ctaaaaatcc ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac
    16741 tgacaatatc gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag
    16801 cagcttctgt ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa
    16861 cagcttccgt caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt
    16921 cacgactaaa catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat
    16981 acgcagtatt tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg
    17041 gcgaatacga tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga
    17101 tatgattgac tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg
    17161 caagattggt ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg
    17221 ctgacacgat attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa
    17281 tattcaattt ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca
    17341 tactttcttc ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa
    17401 cgatccatac aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg
    17461 ctaaaagtgc ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg
    17521 tagccatcat gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg
    17581 ccgtcaaaac tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac
    17641 tgtaatacca tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg
    17701 cgccgattgc tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa
    17761 atagccacgg tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg
    17821 cacctgtaac tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa
    17881 aagtagcgat aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca
    17941 agccactcat ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa
    18001 ttgaggaata catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa
    18061 aggtcgtaga accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc
    18121 ttaagaaaaa aggttttgaa actagaataa gaataatata aataactaat ccccaaatca
    18181 agacattgat tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg
    18241 ccagtgctgg caaagccact gttaagacga ctttcgatag aatttttcca acagattcag
    18301 aaaaaatccc cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga
    18361 ttgcactgac aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct
    18421 ttctcctcct aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg
    18481 tattaacatt tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga
    18541 aacttttgaa acttatgtaa agtgattttt actagaacac aaaaaaaacc ttaaaaccaa
    18601 acttaagaag tttagtttta aggttaactt aaattatatt atgcgtggaa acgttccacg
    18661 ccatctagat aagttgcttc caatgtcata tcagggttca atacgataaa gtctgcatcg
    18721 cgtccttgag cgatttttcc acatgtatca tcgattttac agctgattgc tggaatcaga
    18781 ctagccatca tgactgcttg ttctggtgtt gctaatcccc agtctacaac atttttaatt
    18841 gcttctttca atttcaatat acttccagct aagttgccag attctagacg tgctgttcct
    18901 tcttttacta ctactggaaa ttcgcctaaa ttgtaatttc catcaggcat cccaccggcc
    18961 atcatacagt ctgtaattaa tgcgacatga tcatgtcctg ctttttccat taggattccc
    19021 gcagcatttg gatgtacatg atggccatca caaatcaatt cagaaaagac atgttctaaa
    19081 gataataacg cacccaccat acctggttcg cgatgattca atccacgcat accattgaaa
    19141 gcatggacaa atacacttgc gcctgcttca acagcttttt gcgcttcttc tagtgtagca
    19201 ttactgtgtc ctagagaaac aactactcct tcttcagtca cttgtttgac aaactcttct
    19261 acaccttgac gttccggtgc taaagcaatt tttttgatta aaccacctga agcttcttgc
    19321 cattcatgga aagtatccaa atcaggatcc ccgaaataac ttgggttttg tgcccctttg
    19381 tgttcttctg tgaagaaagg accttcaaaa tagattcctt tgattttagc tcctggtgct
    19441 tcctgataca ttttgccgat tgtctcagct acatctttta aacgttcttt gcttgacgtc
    19501 aatgttgttg gtaaaaatga agtcactcca caagataaca gaccttcaga catcactttg
    19561 atgccttctg catcattatc cattacatca tgattcatat agccgtgaat atgtgtatct
    19621 accaaaccag gagcaatcca tttgccgctt tggtcaatga tgttgacttc tccctcaggt
    19681 agttctttgg tgtattcacc aaaaagtcca tcggtaattt caagatagcc tgcatgtttg
    19741 acacctgacg ctaaaaagaa tttgtcagca tagataaaag ttctcattct attctctccc
    19801 tttgtttttc tacctctaaa gtactcttat ttagagaaga agtcaagaat ggtctacacc
    19861 atttacaaat tttttgaaat ttgtttcaca gcttgtgcta agccttcata gaatgctgct
    19921 ctttctggtt cttctttacg cttgtatatt aagcttttaa gcacaaataa atcattgagg
    19981 taatagtagc tattcttctc ttgagcccaa tagattcctt gattcacctg ttctaacgct
    20041 tcgtcgattt cattttgatg gaataagaaa tttccataat tataaaaaat ttgcgaaaat
    20101 tcactttttg atcgttcatc agctgattga tgcagcagtt ggatgcttcg actcaaatag
    20161 tagcgtgctt cagccgtttt tcctgccaca ccatatattt ttccgataca actaatcagt
    20221 tgtatttctg tgtcagaaac atggattctt tctttttggc tagagtagct taatgcttct
    20281 ttcaaataat aaagggactg agagatattt tgacgcaacg tgaattcgca aacaccgaga
    20341 taataataat agcgttgaaa gtcagttgct tgatacagat tttcagaaat atcagaagat
    20401 tttagtactt gttctaactt ccgataatca cgtttttgga aacaactatc caacagttca
    20461 aataatttat cgtttgtccg agtagttttt cgagagagat tcatcacatg atcaatcgtt
    20521 acccctagcc gttgacaaat ctgcatcatc accaatacat tcggcagttc ctcattattt
    20581 tcgatccgac ttaatacact ttgtgaacaa atatcatgcg aaagcatttt ttgcgtcatt
    20641 ttttgttctt ttcgaatctc tttaatgatc gttccaaagg actcgattaa cgtttccata
    20701 agtatcctcc ccaaaatatg cgtattcgca ttctattcgt attttacttt aggaagtcgc
    20761 aaacttcaag aaaagacttg caactaaata atatacttta gttatttaag aaaactccac
    20821 tagaaagaga gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt tggtttttaa
    20881 aatcattttt ggcgataggt tgctaggaat tctctcattt tcttagctgt ttgtttcaaa
    20941 tcatccattt taggaagata aacgattcga aaatgatctg gctggttcca attaaatcca
    21001 ccaccatgta caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa tttttcatca
    21061 tccaaaatat tgaatcgctt tgtatcaatt tttgggaata tataaaaagc agcttttggt
    21121 tttacggctg aaagaccagg aatatcattg attgcattat agatatattc tctttgttca
    21181 tagatccttc cgcctggcag gagcagctca tcgacacttt gataaccgcc tagcgcggtt
    21241 tggataattt gctgagacaa tacattggaa cacaaacgca tggaggacaa catattcagc
    21301 ccttctatat agcctttgac gtgtgactta tctccactta ggaccatcca gcctactcga
    21361 aatcctgcta cacgatgaga tttggataga ccgttcagcg taacaacgaa caaatcaggc
    21421 gctaatgtag caatcggaat atgttctaga ccatccatca ctaagcgatc atagatttcg
    21481 tcggaataaa taatcaggtt attttgtcga gctacttcaa cgatttcttc taataattct
    21541 tttggataaa gtgccccagt aggattatta ggattaatga taacaatagc ttttgtccga
    21601 ctcgttattt ttgatttgat atcatcaata tcaggatacc attctgcctc ttcatcgcaa
    21661 atataatgaa caggtgtgcc tccagctaat gcaatagaag ccgtccacaa cggatagtcc
    21721 ggcattggaa ccagtacttc atctccattg tccagcaacc cctgcataca catcgtgatc
    21781 aattcgctca ctccgttgcc ggtgtagata tcattgatcg tcacattcgg aaaatgtttc
    21841 agttggcaat attgctcaat tgcttttcta gcagaaaaga tccccttgga atctgagtag
    21901 ccttctgagt cacgggcatt catgatcaaa tcacgaatga cttcgtttgg tgcttcaaat
    21961 ccaaaaggtg cgggatttcc cgtgttcaat tttaaaatac taatgccctc ttcttgcatc
    22021 cgctccgctt cttctagaac gggaccgcga acatcgtaac tgacaccttc taatttgttg
    22081 gatttttcaa attctctcat cacttagcca atccctcatt tcaaaaagtt agaactttcc
    22141 aaattttttg ttatttacta tttcaatatc atactccatc ctagaaaatt ttgccactta
    22201 tttaggaaaa acgtaagtat taaaaaaaca caaacaagcg ttgagaactt aattgtcatg
    22261 atagtcattc catagattcg ctgcccaatc cattgcaatt ccacaaatga ttcctagcat
    22321 aaaaccaagc aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga tcattcccag
    22381 tcctgaaaat tctccgtcat atttctccat cttaataagt cctcccattt ttgagcttga
    22441 caactatgta aaacctcgct atcattagtg tgttattatt acgcagatgt gttggaattg
    22501 gcagacaagc atgattgagg gtcatgtgag cgttagctcg tgtgggttcg agtcccatca
    22561 tctgcactac aactcgtgaa gttaattttg cttcacgagt ttttttgttt gaacgtttct
    22621 ttatgccatt atatacatat cactgtatcc tttacaaaaa gaacgtacaa tcatgtaaac
    22681 agcctgaggt gagtatatga aggtcggaaa caagctactc gtagaaactt gcaagtgtta
    22741 aaacaattaa tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga gggatttttt
    22801 gtatattatc tgtttatcac cgttattata aaggtacaaa taccaagcac ggttaatcca
    22861 aattgcataa attcattttt ttgtacactc atgcttttca ttagctcttt aactgccatt
    22921 agtactaaga tggcgataac aacataaagt atatagtacg tccgcattcc ggaagcccat
    22981 actgtgggag aaaatcccat aattaacctt gaacaaaatc ccattatcat caaaataact
    23041 aaaaaaatag aagttttctt attgtcaaaa cttaaataaa ttccaacaat taaacatagt
    23101 aataatgcca agactactaa agtagctatc caagtacctg gataaaaaag tgacagtttc
    23161 gttcctaact ctgtaaagag attattcagg ttactactac tccaaatcat tgccctttta
    23221 tttccattga cgtaagtaaa gctttgaccc attgtattac caaaataaat aatcaggttt
    23281 aagaaaaacg gtaatgcagt caatatacgc acataatagt tttgacattt cctatatgtt
    23341 agaaggaaaa ttaagaaaaa tagcaataaa aataagatat tca
//
LOCUS       DACEJR010000045        22337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEJR010000045 DACEJR010000000
VERSION     DACEJR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.045"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW651_001823"
     CDS             <1..156
                     /locus_tag="IW651_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0814545.1"
                     /translation="RKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQM
                     LSQPMEN"
     gene            534..2576
                     /locus_tag="IW651_001824"
     CDS             534..2576
                     /locus_tag="IW651_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0814546.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2587..3207
                     /locus_tag="IW651_001825"
     CDS             2587..3207
                     /locus_tag="IW651_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0814547.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3219..3677
                     /locus_tag="IW651_001826"
     CDS             3219..3677
                     /locus_tag="IW651_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0814548.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3696..3974
                     /locus_tag="IW651_001827"
     CDS             3696..3974
                     /locus_tag="IW651_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0814549.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3999..5366
                     /locus_tag="IW651_001828"
     CDS             3999..5366
                     /locus_tag="IW651_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0814550.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5381..6526
                     /gene="dgoD"
                     /locus_tag="IW651_001829"
     CDS             5381..6526
                     /gene="dgoD"
                     /locus_tag="IW651_001829"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ0814551.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6553..7044
                     /locus_tag="IW651_001830"
     CDS             6553..7044
                     /locus_tag="IW651_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814552.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7487..8686
                     /locus_tag="IW651_001831"
     CDS             7487..8686
                     /locus_tag="IW651_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0814553.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9181..9939)
                     /locus_tag="IW651_001832"
     CDS             complement(9181..9939)
                     /locus_tag="IW651_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0814554.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10107..11042
                     /gene="pfkB"
                     /locus_tag="IW651_001833"
     CDS             10107..11042
                     /gene="pfkB"
                     /locus_tag="IW651_001833"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0814555.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11039..12484
                     /locus_tag="IW651_001834"
     CDS             11039..12484
                     /locus_tag="IW651_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0814556.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12512..12967
                     /locus_tag="IW651_001835"
     CDS             12512..12967
                     /locus_tag="IW651_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0814557.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12986..13960
                     /gene="lacD"
                     /locus_tag="IW651_001836"
     CDS             12986..13960
                     /gene="lacD"
                     /locus_tag="IW651_001836"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0814558.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14071..14632)
                     /locus_tag="IW651_001837"
                     /pseudo
     CDS             complement(14071..14632)
                     /locus_tag="IW651_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14776..15390
                     /locus_tag="IW651_001838"
     CDS             14776..15390
                     /locus_tag="IW651_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0814559.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15631..16014
                     /locus_tag="IW651_001839"
     CDS             15631..16014
                     /locus_tag="IW651_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0814560.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16377..16886
                     /locus_tag="IW651_001840"
     CDS             16377..16886
                     /locus_tag="IW651_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0814561.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16986..17636
                     /locus_tag="IW651_001841"
     CDS             16986..17636
                     /locus_tag="IW651_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814562.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18087..19025
                     /locus_tag="IW651_001842"
     CDS             18087..19025
                     /locus_tag="IW651_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0814563.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19038..20090
                     /locus_tag="IW651_001843"
     CDS             19038..20090
                     /locus_tag="IW651_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0814564.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20356..20991
                     /locus_tag="IW651_001844"
     CDS             20356..20991
                     /locus_tag="IW651_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0814565.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21182..21973
                     /gene="msrA"
                     /locus_tag="IW651_001845"
     CDS             21182..21973
                     /gene="msrA"
                     /locus_tag="IW651_001845"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0814566.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7119 a         3547 c         4702 g         6969 t
ORIGIN      
        1 cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt tcttagtctc gtcttttgat
       61 acttacaacc aaaaattcct aggtcgcagt cataaaggtt tccaacaggc cgaaggcgag
      121 ctcgaacaaa tgctgagtca accgatggag aattagaaac aatctacagt agggaagaac
      181 catttacaca aaattattga cgctcccaaa agcattgttt ttagcagttt aatattattc
      241 tttttcaatt agactgactt gatttagtta cggaatagat actttaatgt gttgaagaag
      301 caacgtcccg tgtttattta ctaaagcatc caatattcta ccgaatattt aatattcaca
      361 ttagccctaa aataattgtc ataccatgtt acatttgata taacagcgtt cattttaaaa
      421 atagctgtta gtgtgacaaa acattaaacc aagtgtgaca aaaaacgtga tgaaagcttt
      481 ccggaaacgt atgtgaacaa gtatataatg attttgtcag gaggatagtc aaaatgaata
      541 aacgaattcg tgaaatactc aaactgatta ttaaaaaccc tgaaatgaaa ctttccactt
      601 taacaagtga acttgatttg acacgtcgtc aaatcaatta cgcgatcaat caatttaatg
      661 aagaattgaa aacaaagaac attccgttaa ttcagagaag tcattctgga aatattacgg
      721 taccaatcga ggttatccaa atgatatcac agcctgatta cgacataggt gatgatcagg
      781 aacttgtcgt tctcactgat agtgaaagac aagcattgat agtgatgact ctgatcgcta
      841 atatcgatta catcagtctg gatcatttgg tagatgcggt tgatgttagt aagacgactg
      901 ttatggaaga tatcaaacga acggaaccgt tcatcagaaa ttatagtctg acaattcgat
      961 ataatcggat gaatggttac caattggttg gttcggaaca ccgtatctta cagctgttat
     1021 cggatctagt aaaacggttt ccgatttttc gccgagaaat gattcgcaga aaattggcac
     1081 aagatgtttc tgaagaagaa atcgtccatt tgatccataa catggaacaa atgttgcatc
     1141 ttagttattc tgatgagtcg gtagactatt tgcagacatc agcgagattt ttagtaagcc
     1201 gtgggatcaa gatgtcagat aagcacgcgt tttttcccga tgatgtgacg gatactcctg
     1261 aattccgaat gctgatgatc ttggtacatg aaacaggttg gagtctatcc aaaagttata
     1321 tggagtggtt cacgttatta tttttaactt ccaatatttt tgagagcaaa actactcaaa
     1381 attatgattc ggatcaagaa ttgcgaaaat tgatcagcca aatggttgaa aatttccaaa
     1441 atcaaacgtt gattttgatc gatgaccgag aagactttga acgtaggata ctgagtcatc
     1501 tgcgtccagc ctgttttcga atacgctata acttgagttt aggagtttat tcaatagata
     1561 gtctgatcaa ggatagtaat catgcgattt tgattgattt gatgaaagaa ttgattattc
     1621 ctattgagaa ttggctaaat aaggcctttc caaatgatga attagatctt ttatcctatt
     1681 actttggcta tcaattaagt aatcataata aattgaataa gcaacgacaa agggctgtag
     1741 ttgtttgtac taatggtgtc atggtctcaa agttagtacg agccaatatg gaaaaacttt
     1801 ttccagagat ccatttttta gcttccctat ctgtgcgtga tttctacaaa tttgaagctg
     1861 attatgatct ggtctttact acgacgccac taaaaagttc tatgctgcag tttatgatcg
     1921 agccaatcat gacatatcag caacaaatca gtttacgata tcgagttttg agtgatttag
     1981 gaattactaa ggttgatcga gcaatggatg aattgttaac aatcattagg aaatatgcaa
     2041 atatcaccaa tccagtttcg ttgcgagaag aactgcagta ctttttgatc aaggaagatg
     2101 gaagcacgcc gctggacaac ttcaatgtat tgccttcatt gacttattac ttgcggccga
     2161 gttatgtaca aatcgttgaa gaagagatgt cttgggaacg agcagtggag gtggcttgcc
     2221 gaccgttatt ggaacatcaa atcattgact cgcgttttat cgctgattgc ttggatcaaa
     2281 tcagacaacc gggatatgca ggttacttgg gaatgaagac ttgcattccc catacaacgg
     2341 tcgataaagg tgtaatcagt gacggtgtca gcattttagt ttgtaaaaag ccgatacgat
     2401 ttcctaatgg tgaagatgtc agcttgattt tgccgctttc attttttgat ttaacgaagc
     2461 atctgcgagc catcaatcaa atcgctgata tcgccaaaga tgattcattg attgagcagt
     2521 tattgattgc tgatgacgaa aagacagttt atcaactatt aagacaaaga tcttaaggag
     2581 gaaaacatga gactagcaga ttatcctaaa ttgacagtga tcatgcgtgg ttatactttt
     2641 gatcaggcag atgctatttt acaagcaatg caggaatttg accatcaatt tgccgtagag
     2701 atgacaatga acacaaaagg agctcttgaa aatatacatg aactaaatca acgttatggt
     2761 gacacgactt atatcggcgc tgggacggta aggacattag aacaagccca agcctgctat
     2821 gaggctggtg caaaattttt attaggacca catatgttca caaaagaaat gttgtcttac
     2881 gccaataaga aaggattatt ggcggtacct gccgcaatga ctccttcaga agtcgaccgt
     2941 atgtttaaag aaggagctga tattgtaaaa gtatttcctg cagcggttgt tactccacgt
     3001 tttttcaagg atattcaagc acctttaggt aagttaccac taatgggggt tggtggaatc
     3061 agtaaggaga atgcgaaaga gttttttgaa caaggtgcta gctatttagg tcttggttcc
     3121 ggtatgttca acaagcaaga catcgaagag ctaaatgtta aaaacttagc acgttcgatg
     3181 aaagaattaa ttgaggctat taaataggag tgaggacttt ggaactttat ttgaatgagg
     3241 aactcgtttt tcgaaatgtt caagcaaaaa cagatagtga agtcttagcg tttttagcaa
     3301 gcgagatgta caaaaaaaac tacgtgaagg aggaatatat tcaagcaatc caagaacggg
     3361 aaaaagaata ccctactgga ttaccatcaa caccaccagg aatcgcaatt ccccatgcaa
     3421 actatgaaat ggtcaataaa acaactttag caattgcgac tcttaaagat ccggtcctat
     3481 ttcataatat ggaagataac aatgagcagt taccaatcca aattgtgatc atgatggcga
     3541 ttggggaacc acatgggcaa gttgaaatgt tacaaaaaat tgttggaatc atacaagatg
     3601 agccactgcg gcaagaaatg atcagagcag gcaatgatac tgaattatta gaactattaa
     3661 agaaagcggt tttttaatat aaggaggaaa tcataatgaa acgaatttta gtagcatgcg
     3721 gaaatggtat cgccacttca acagtagtag caacaaaagt tagagagtat ttgcaacaac
     3781 atggcattga tgtttcaacg actcaaacaa aattgatgga agttccagga aaagttcaag
     3841 actatgatct attggtgacg actggtcaat ttgatggtca aacaggtggt gtaccagtaa
     3901 tcaaagggat gccgatttta acaggtatcg gtgccgatca gacaatggaa gagattttga
     3961 acttactgaa ataatagctg atcaaaggag ctgaaaaaat gaaaatcatt atgaacgtct
     4021 ttaacttctt tattgatgct ggaccaacag ttatgttgcc agtcatcatc acgattattg
     4081 gtttgatttt tgggttaaag atttctcgtg cttttaaatc aggtttgaca ttaggtatcg
     4141 gttttgccgg gatcaagttg atattggact ttatgacgac caatgttgga ccagctgcca
     4201 aggcgatggt tgatcgaact ggtgttaaac tagatgcttt ggatgttggc tggggttcga
     4261 ttgcggctgt tacttgggca tcgccaatca ttccaatctt gatttttgcc attctattgg
     4321 tcaatatcgt tttgctgatt ttgaaacgta cacatacgct ggatgttgat atctggaact
     4381 atcaccatat ggcaatcgtt ggtgttatag tgtattttgt gactaagaat gtcttcttag
     4441 gtgtaggtgc atcagtagtg atggcaattg cgacttttaa gatttcagac tggtcgcaac
     4501 cgatggtaga aagtttcttc ggaatccctg gtgtttcttt accaactgtt tctgcgcttt
     4561 catcactggt tattgcttgg ccgttaaatt ggctgctgga tcgtatcccg ttattcagaa
     4621 aatcaaaatt caccatcaaa gatgcacaaa aatatttggg attttttggt gattctatga
     4681 tcatggggtt agttattggg atggtgattg gtgttttagc cggctatgat atcaaagcgg
     4741 ttttacaatt aggtgtcagc atgtcagctg ttttggtttt gattcctaag atgacttcat
     4801 tatttatgga aggattgatg ccaatttcag atgctgcaca aaaatggtca caaaaaaaat
     4861 tcaaaggtac acgtttattt atcggattgg atgctgcagt tgttgtaggt aatccagatg
     4921 ttatcacaac agcattgatc attattccac taacgattgc attagcttta gtattgccag
     4981 gaaaccgtgt gttaccattc gcagatttag cggttgttcc tttccgcgtg gcaatggtcg
     5041 ttgccttgac tcgtggtaat ttattaaaaa atatcattat tggcttagtt gtgacagctt
     5101 cattgctatg gtgtgggtca gctactgcgc caatgctgac agcaattgcc aaatcggttg
     5161 gtattaactt aggaacttct tcaatgttaa tctcatcatt tgctgcgact tccatgatcc
     5221 aaagttattt ggtatttatc gcatttgctt acaaaccaat catcggtatt ccagtgttgt
     5281 tattagcctt tgcgggcgca tggtattact tcgatggtat caaaaaagtt aatcatgaaa
     5341 tggatgaacc attgaaggca caatagtagg agggtttaaa ttgaaaattt cagatgtcac
     5401 agtgtataaa gtaaaaccaa gatggatttt tgttaagatc tcgactgacg aaggaatcga
     5461 aggctgggga gaaatgatct caggaactaa aacagaaacg gttgttgctg gagcttatga
     5521 gataggaaac cggctgattg gacgtaatcc gtttgaaatc gagcgcctct tccaagaaat
     5581 gcatcgatct ttcttccgag gcggtcctat taatggaacc atcgtttcgg gtttagaaat
     5641 ggctctgtgg gatatcaaag gaaaagcgtt taatgttccg gtttatgaat tattgggcgg
     5701 tgcagcacgt gataaaatca aggtttattc ttggattggt ggagatcgac caagtgaagt
     5761 tgcagaacaa gcacaagatc ggtttgatcg gggctttacg gccgtcaaga tgaacgcgac
     5821 agaagagctg cattatatcg attcctacca aaaagttgat gaagtggtgg aacgagttgc
     5881 gtctattcgc gaacgttttg gtgatgcaat ggatattggt gttgattttc atggacgggt
     5941 ccataaaccg atggcaaaag tgctagcaaa agctttggag ccattccatc caatgttttt
     6001 ggaagaagtc gttttacctg aaaatgaaga acactttaaa gaagtagctg attctgtggc
     6061 tgtaccatta gctactggtg aacgcttgta cactcgttgg caattcaaga acctctttaa
     6121 acaaggtact gttgatatta tccaaccaga tgtggctctt tgtgggggaa tcttggaaac
     6181 ccgtaaaatc gcagcgatgg cggaagctta cgacatggct gttgcgcctc atgcgccata
     6241 tggtcctgtt gcactagcag caactctgca agtagactca tgtacgccaa acgtctttat
     6301 ccaagaacaa agcttaggga tccactataa caaaggtttt gatctgttag attttgtgaa
     6361 gaacaaagaa gttttccaat ataaagacgg ctacgtagat ctaccttcaa aaccaggtct
     6421 gggcttggaa atggatgaag acaaaatcaa agaaattgca caagaaggtt tgatttggac
     6481 caatccgcaa tggaaaaatt atgacggtac gatcgctgag tggtgataaa tgaaaataaa
     6541 ggatgatgaa agatggcaga agcacttatg atcacagttt ttttaggact gctgattatt
     6601 ttgcaaataa tcgatcaaaa gaaaattatc gtgtttgctc agagtagtcc tgtaaggaag
     6661 agtttgagta ttttactgac agtcgccatc ctgttgattt tctggccgga acatctgact
     6721 gatcagatta aactggttgc ttttgcttct gtcattttga tcaatggttt tttgaaggag
     6781 ggtctagcaa aagattctgt tgttagattt ggagtattga acggtaactt taatcgatat
     6841 cctcaagtcc aagtagaaaa actaggtgac gataaatcat ttatttcatt ttacaaaagc
     6901 aagaataatt ctttttcaat gatttttgat aaagatcaaa ctgaaatgat ggatgtcttt
     6961 caagaacttg atatccattc caaaattact ttagatcaaa gttcggaaga aaaaagagca
     7021 gttgtaaaaa aagataatga atgattctac taccgaaatt atgatttctt tatcacgaat
     7081 aatgaaactt gttttaatga atcatattga ttgaagtata caatgtatat taagttttgg
     7141 aaagaaatat attggtacaa gatatagcag gaatcgatta ctcgatccct gcttttttta
     7201 acttcctttg cagcaaaaaa agctcgtaat ctaagagatg atggatagtt tttagaatat
     7261 atatattttc ttcaattcca gaaaaaaatg accaagctag tatgctaaaa aagtacgatg
     7321 cttctatgat cttttttccg tctttattaa cagatgtttt aattgatcaa cgcatattgt
     7381 aatctaccct aatttccata gttcttcacc acttttaaaa atagtaaaac ccctacaagt
     7441 atgctatagt aaattgctaa agaaaaacca ataaggtggg agatgtgtgc gcgatcaaac
     7501 aagcggaaaa atgattcgtt tttattatac aagtttgcat tttctttatt ggatgatgtt
     7561 ttgttctgtc tatgggtttg cgacattgtt tttgatttat ttcaagattg atccagctaa
     7621 aatcggaggg atcttagcat tggtcaatat tgtttcaacg gtgcttcagc cttttgtcag
     7681 tcaagtgatc atcaagaaaa gaaagatccc actgatcacc gtgctgaaga cgatgatagc
     7741 aggattagga gtgattctct tagctggggt attgattccg aatttgccga tcattactta
     7801 tatcttgggt gggattttag tagtttctat gcaatcattt ctcaatgcgc tggcatttga
     7861 gtattcaaat accggctaca ggatcaactt tggcttttca agagctggtg gttcattttc
     7921 ttatgctgta acttcttttt tactgggaca gctgttagcg atttattctg ccgcgatttt
     7981 gccaatcttg gttatagttg aagcagtgat atttttcgtt attttgcatc tgctgccgga
     8041 tgtaaaaaat caagaagttt ttcctatgac aaaagaacag gatcgctcag tttctttgag
     8101 aaaaaaatat cctttcctga ttttgttatt tttaggtttc agttttcttt ttactttcca
     8161 caccatgatc aacagctttt tggcacagat ctttgaaaat atccacggcg gcagtaaaga
     8221 agttggcatc gcattgatga tcgccgcttt atgcgaatta ccgggaatga tcttttttga
     8281 acagcttgtc aaaaagaaat ccagcaaatt ttggctgtct gttgccagta tttcatttgc
     8341 tgtcagagga ttcatgatgc tgctggccag ttcgatcctg atgatggaaa tgacgcatct
     8401 cctacaagcc ttcagtttcg ctttgtttat tccagcttcg gcttatttgt tcaatcaatt
     8461 tctggcggat gatgatcgtg tatttggaca aacgatgatc atcatcgcca caactcttgg
     8521 tggggtgata ggaaatgtct tgggcggcag tctgctgcag catttcggtg tatgggagat
     8581 gctgattttc ggtactggtt ttgcggtgct aggtgcttta ttaactattt tgggcattcg
     8641 aaaaataccg aagacaggaa acgatagtga ttctcttgtt gagtagcata aaaatactta
     8701 gatactagtt ttatataaat aacatatagt gattattggc gtaattacaa aaaacgagaa
     8761 taacttaagt aagcatttct aaataattga gaaacctcat ctaaaaagat aagaaaatat
     8821 ccctaataaa acttctgtaa cattttagat aactgattgt aatctgaata ataaactata
     8881 aggagacggt tagacggtct cctttttttg cgtattataa ttctagaatt gttggaaatt
     8941 gataataatt aacaagaaat attagacagg aacttcgaac tgcttatcac attcttatta
     9001 ttatatattt gcgtaagtag tcctggtagt ctcaatgcac tattctatct tgaaactaag
     9061 aagttataga aggagcaatg atagaaagca cgagaataca aatagcgatt aaatattttt
     9121 ctagacaacc ttagttaatt cacacagata tattttaggt acagtcttac agatatagag
     9181 ttagtgctgt gtgaatcctg atgttcgcag tgcataggag tcaagtaaga ttctagccag
     9241 tatagaaagt ggaagtctgg agcgtacctc ataatctggg aagaaggagc cttctgattt
     9301 aaagccaaca aaaggaatct cgatatgttc catcaaactt gaatgacgat tagcagtaag
     9361 caaaattgtc ccgatctcag ctttataaca gttttcagct gcggtagtca attcctttgt
     9421 ttcaccgttt agtgaaacaa agatcacgat gtcatctttg gataattttc tactaatgtt
     9481 ttttatgata ttgggatcag tatgcatctc acaatattta ccggtaagtt gaaatttgac
     9541 catcatttcc tgagcaatca attctgaaaa acctcgggca aaaatcatga ttcttctagc
     9601 agccttgatt cgttgaatag catcttcaat attcccgctt tctaccatat tgatggtgcg
     9661 gattacctct tgctcatttt taagaatagc agtctttatt ccttcatcaa ctttatcaag
     9721 agcggcaaaa ttgatatttg tattttcttt ttcttttaac gaatgtttaa aagacgtaaa
     9781 cccctcatat ccttttttct tcatagtacg aacgacggtt gctgttgaaa cgttggcata
     9841 ttcacttagt tttacaatgg aataggaggg gatttcgagt atgtgacttt gaataaaatc
     9901 ccagagatat ttctccgatt cacttaattt tgtatccata ttttgcctcc gattagttag
     9961 gataattcta ttgtaaagcg ggagtttcct ttacttcaag tttatttaat atattttgta
    10021 aaagttttca atgaatttag tcatttatga aaacatttac attcgatctt gatacataat
    10081 gaatatgtaa gaaaggagga gcgtaaatgt cagtttatac atgtacttta aacttggcaa
    10141 tcgacctttt tatcgaaaca gatgaaatga aaccatccca agtcaatcga acaaacgatg
    10201 atgatataca agctaatgga aaaggtgtga atgtttcatt gatcatgaag caattaggta
    10261 tcgatagtac agctttagga tttaaagcag gttttaccgg gaattatatt gaggattatt
    10321 tgcatcaaaa agatattagc actgatttta ttagcgtacc gggaatgact agaatcaacg
    10381 tttttacaca agttaataaa accaatgaag aattcaaatt ggttaataaa ggacctgaga
    10441 ttcctcagga acaaatcaaa acttttctaa atcaaataca aaagctgcaa gctgacgatt
    10501 atttgtgtgt ttctggcagt ttgccgcgag gggtctcacc cgaaattctt attgatatca
    10561 gtcgtatttg ttttgaaaat aaggtccatc tgattattga ttcaagctat ccggaaataa
    10621 ttgattgtct gccgtatcag ccgttcttat taaaaccaaa cgaggaggaa ttgcaatctt
    10681 ggtataatgt cgagcttagt gatcgtgaag gatatattca ttatgctgaa cgcttgattg
    10741 ccgatggagc gaaaaatgtt ctcctttctc taggaggaga gggaggaata tttttaaata
    10801 aggatatcgt ttattctggg aattctccta aaggtaaagt agtcaataca gcgtgtgctg
    10861 gtgacacttt gttgggaaca tttcttgcag gatatttaaa aggtgaacag gtagatatta
    10921 atttgaagaa aagtttagca gctggaagtt caaccgcttt tcgtaaagga ttaacagatt
    10981 tttcggatgt tgcagagttg gaaaaacaaa tcgaaatcaa agagggagcg agtaaaaaat
    11041 gacaacttac aaaattgttg ccgcaacagg atgtccaaca ggaattgccc acacttatat
    11101 ggctcaagaa gcattggagc aggcagctaa gcgcaaaggg gtatctatca aagttgaaac
    11161 ccatggtcaa gtagggatag agaatgaatt aacaccagaa gaaataaaaa acgctgacgc
    11221 tgtaatcatt gcagctgaca aagatgttca agcagaacgt ttcgcaggta agcgtgtaat
    11281 cgatgtgcct gtcagcaagg ggattaaaga tgctgaccaa ttgatcgaag atgcgttggc
    11341 tggtaaaggt tcaatcactg cagaaaacaa aacagttgac gctgctggtg agaaagcgga
    11401 gatgggggtt gcagcaggca ttggtcacag tatttataaa aatctcatga acggtgtttc
    11461 tcatatgctg ccgtttgtag tggcaggtgg ggtaattatt gcaatttcgt ttgccatctg
    11521 gggtgtgtac tcttttgatc ccaatgatcc tacttacaat ccgactgccg ctatgttaaa
    11581 gggtgttggt gatgcggcaa tgggaatgat ggttcctatt ttagcagctt atatcgctga
    11641 aggaatcgct aaacgtcctg gtttggttgt tgggtttgtt ggcggactgt tagccaattc
    11701 tggcggagcc ggtttcttag gcggaatttt tgcaggtttc ttagcaggct actttatctt
    11761 actattgcaa aaggtcatga aaggcttgcc taaacaattg gatggtctga aggcgatctt
    11821 cttatatccg gtgatcggtg tcttgtttgt tggtgtagtc atgtctttat tagcaaatcc
    11881 aatggaagca gtcaaccaag gaatgatgaa tttcttggca ggattccaaa attctaatcc
    11941 attaatttta gggattatcg ttggctgtat gtcagcattt gatatgggag gtccagtcaa
    12001 taaagcggct tatgttacag ggactgcgtt gttggcacaa ggtaaccata catttatggc
    12061 aggggtttcg gcagcttgta tcgcgccccc attgattact ggatttgccg cactgttttt
    12121 tggaaaatat ttcgacacga atgatcgcaa cgctggttta gtcaacttta tcttgggctc
    12181 aacgcacatt accgaaggag caattccatt tgcggctaaa gatccgctgc gtgttctgcc
    12241 aactatgatg ctaggttcat caattgctgc tgtactgaca tatatgtttg gtgtccaagt
    12301 tcccgcacct catggaggtt ttctagtatt acctgttgtt actggtaagg ttgcatgggt
    12361 agggtcgatt ttacttggtt caatcgttgg cggagtaatt ttagggttga tccaaaaacg
    12421 tagtgtagaa aaacgtgaag cagaagcagc tgctgttgaa atgaaagaag caaccaacaa
    12481 ctaaaggtta ttctagaaag aaggatttca aatgactttg atcaaaacaa ataaagtttt
    12541 tatgaataaa aattttacga cgcaagatga ggtaatgaac ttccttgcag atcagtcaat
    12601 tgtcttgagc attagtgaag accgagatgc agtttacgaa aaattgatgg aaagagaaga
    12661 agaaagcacg actggtatga tggatggttt tgccatccct catgccaaag atgctagtat
    12721 caaggatgcc gatattgtga ttgttcggtt aaacggtaag gtcgattgga aaagtttaga
    12781 tggagaagga acggattttg taatcgctct gtttattcca gattcagagg ctggaacaac
    12841 ccatctaaaa tttttatctc aggtagccag acttttgatg cacaaagatg tgacagaagg
    12901 tctgaaacaa gcccagtctg ctgaagaaat agctgaactt ttaaaccaaa aattagaaga
    12961 aaactaaaaa ggaagtagtg aaatcatgcc tttaacagaa aaaaagaaaa cttatattcg
    13021 tcaactaagt aatgaaagtg gcatcatcag tgcgttagca atcgatcaac gtggtgcctt
    13081 gaaaaaaatg atcggtaaat ttcaaaataa atcagcaacc gatgaacaaa tcatcaattt
    13141 caaaaaaatc atttcttctg aattgactcc atatgcttca gcaatcttat tagatccaga
    13201 atatgggttg ccggcagccg aagttcgcga taaaaatgct ggtctgttgt tggcctatga
    13261 aaaaactggt tacgatgctt caactccagg acgtttaccg gatattttat ctatttggtc
    13321 tgtaaaacgt ctaaaggagc aaggggccga tgcttgtaag ttcttgttat actacgatgt
    13381 agatgaatct gatgacatca atgaccaaaa gaaggcgttt atggagcgga ttggttctga
    13441 atgtgttgct gaagatttgc catttttctt agaattggtt tcttatgatg ccaacgatcc
    13501 tgatacttct tcgaaggaat atgccttgaa aaaaccgcat aaagtaaatg aaatgatgaa
    13561 ggaattttct aagcctcgct atggagttga cgttttgaaa gtggaagttc ctgttaatat
    13621 gaactatgtg gatggttaca ctaaaaatga agttgcttat accaaagaag aagctgctgc
    13681 ttatttcaaa gaacaatccg agactaccaa cctgccattt attttcttga gcgcaggagt
    13741 aactgctgaa ttgttccaag aaaccttgaa atttgcaaaa gaggctggct ctacattcaa
    13801 tggggtactc tgtgggcgcg caacttgggc gaatggtgta gaaccattcg ttactaaagg
    13861 tgaatatgct gccaaagaat ggatgcagac acaaggacgc aagaacatcg aagagctgaa
    13921 taatgttttg aaagaaacag caacagctat taaattgtaa gaaagcttta actataacct
    13981 taagtttgta acctaatgat ttttaataat caatttgagc ttttaaataa agaattggaa
    14041 gcgtcccgac gcttcctttt tcgtttgttt ttaaggcatg ataggttcta tgttgtcgtt
    14101 caataagcgc aagaaggtca gcattttttt cttagagtat gcaaaattgc ttttctaccc
    14161 aatgatttaa aacggagagg cacccttgga tatggtagat ggtgaagtaa cgcatttctt
    14221 ctgtgatcac ggtttttttg tcttcttgta gccagatttt taaataattt tcttcgatca
    14281 gattatttaa tctcttttga aattggatat ctccgtggtt gatgacgaat aacttgtata
    14341 cgaacgtatt cgtatagaca tagtcgatca attgagcgta tagatcctcg tagctatgtg
    14401 tcgtatcgta ggaaatgatt tcatccaagt cttggaaaaa gctattttcc agtttttcat
    14461 aaagatcata gatatccgta taatgtgtat aaaatgtcga acggtgaaga tcagcagttt
    14521 cggtcagttc acggatggtg atgttttgaa tttttttatc tactaataat tttgataatg
    14581 cttctgctaa cgctttttct gtttttttga cgcggcgatc ctcttttttc attttgcagc
    14641 ctcctaatca acataatcaa ggaattgtgt cggttatccg acaatttcac cgctatctga
    14701 acatagataa ccgacacctt gtctattata atataagtat agcatatcaa tatttaaaga
    14761 ataggagaat aagtgatgga attagccttt aagcaactgg aaaagaaaga ctacaaaaaa
    14821 gccattcaat tcgcgattaa aggtatgcac tttgattggt ataccaagaa caaactcctt
    14881 ttgaatcttt atggaagata cttttggtat ctagaattga atcgcgcgac acaaatcatt
    14941 gctttatatg cggatgatga gttggcaggt gttttattag cagacatcaa aggaaaatca
    15001 aaaaagcatc attcatttag tcaaaagtta tatgttaaac tatttgattt cttacaaaat
    15061 gcatttgtga aagacagcaa aggcgggtat gacgacacca atgacgaatt gctttcagac
    15121 tatttaaaaa aacattcccc agatggagaa attgtctttt tagctgccaa ccctgattta
    15181 aagatcaagg ggatcggtag taaattatta aaagaatttg aacgccgaga gcaaggaaaa
    15241 gaagtatttc tgttcacgga tagcggctgt acgtatcaat tttatgaacg acgcggtttc
    15301 gaacggaaaa aagaaaaaga taacgtcatg aagatcctta ataaagaagt agatctccgc
    15361 tgtatgttat atagtaagat cattcagtaa tgttttttac tatcagaaag cagaagtacc
    15421 acgaaggtca atggaaaaag cgagtaattg atccatttag atgaagatag gtgtagtctc
    15481 aaagatatta gctggcaaag atatccccag ttaaaaatag atttctgtat ccaacaataa
    15541 aaatattttt gtatttatta gtatatatac tttacaaaaa tttgtatata tactaatttt
    15601 tttgtaggat caatgaaatg aggggaaaat atgccaaaat ataaagaaat agccttggct
    15661 ctaaaagaga ggatcgttga gggaaaatat caggaaggtt ctaaattacc tgagcaagaa
    15721 agtctggcta gagagtttaa aacaagccgt gttactatcc gcaaagctat acaactcctt
    15781 attgatgaag gtttgttata tacgagacgt ggatctggta cttttatacg aagcaatatc
    15841 agacaggaca atgagaatgt cactcaagta aataatgtat ttggcacttc ctcacaagaa
    15901 aatacaaatg tgacaagtaa aataatccgt tttgatgtcc ggtttccgac agattatgaa
    15961 aggcggcttt ctgcaaccta cttccgatcc ccttcctgca aatcatgggt ataaaaaata
    16021 ggcatattaa gcgggctagg aggagaaata ttcacatatc attttttcat tccgcaagct
    16081 gcgtcaagtt atttggaatt tgtagaacag atgagagagg tagaagcagc actacaaact
    16141 acttttcaat aagtaggaat tatagaaatt ttactcaaaa aatcttcatt aaaaaatctg
    16201 actaaataag ttgagaaaac agaatatact tcaatatcca gtgatttata aatatcattc
    16261 actcatgata cccagctccg tatcataagt gatgcggttt ttatcattga tcctaaaaat
    16321 cttagcaaga caaaaagtac gtaagaaagt aaagactaaa tacttcggag gaacaaatga
    16381 ataacaatga taggttattg cgattgcgtt atgcgattga tttgaaagat acggatttga
    16441 ttaaagcttt tgagctaggc ggtgtaccac tgactaaaga agaagctcag gcggtactga
    16501 ccaaagtaca ggataagaac aaagataatg cagaaaataa tgtgtatgaa aaaaccatca
    16561 acaaccaagt ttttgacgca ttcctaaatg gcctgattct cttggcgcgt ggtccgcaaa
    16621 aagataagcc gatagtagac acgccgcaaa aaagcaagga aatcaaatat atcaacaatg
    16681 tgttgctaaa aaaattaaaa atcgccttgt ctatgaccac tgatgatatt ttagaagttt
    16741 ttgctgaggc agaactctat ccatcaaaag gcgaaatcgg ggcatttttg agaaaagaag
    16801 gccaacgaaa ttttaagcct tgcggagata aatatatgcg taattttctc aaggggttag
    16861 gaatttataa tcggagaaaa gtataaagaa atttcatgag aaatcaaaac aatggttgtt
    16921 ttgatgagag ctagtttttg tatgagatta aaatattatc ttaatcgagg agctggattg
    16981 gttttatgaa tgatgaatat aagaatgatg aagataagat gctttttgaa gagatagaga
    17041 atcgatgtag gctcaatttt gagcttcggg gaaagatgtc tttaatacaa caaaaaaagt
    17101 atttagcgaa taaatcagag tttactctgg gacatgttga aaaattaatc tcggattgga
    17161 ttagtagtag aagcgaattt acaaaaataa aacagccgat taaatttgac atgaaaaaac
    17221 ttttgttaaa taagagtgag ataggtaata gagatcaata tattagagct aaagggcaag
    17281 aaataattga ttcattaggt gaaatgaggt catacaatta tttgtatgta acacatagag
    17341 ctgatggaat ggtcattact gtggggaaat cttcttctaa tgatattttt ttagatggag
    17401 atctttttta tcaactcaat acaaatcact tgtctggcac tgaaaatata attttaagga
    17461 cggaatatgg caatgaaata tttgcaaaat atgatgagat tttaaaaaat tatttggact
    17521 gggcttggat aattccggtt gagtcaggag atgctaagaa actggagaga ttattgggcg
    17581 atgaactaat taataaaaaa gtaccaattt taaattatta ttcccataga caataaaagg
    17641 ctatttttca tgataatact agttataact gtaaaaatgg aagaaaatct gggaaagaag
    17701 tttttaaata agtgtattgt tcgattttaa taaataataa gcaataccat ccgtctgtgt
    17761 tgcgcaacat cgtactacaa tgaatatttt ttcacaaaaa gaaaatcatc catcgaatga
    17821 atggaaggtt ttagtataca cgaggttaat gccccctctt ttataatcag aggagtgagg
    17881 ttttttctat gaatgtttct attttcttat tgctagtcta gtttctctta cgatgaaatt
    17941 gggattctat tccagttttt attgatttgc tgataaaata acttgaatgg tggatataaa
    18001 catgataaat aagagaagaa taacaaaaca aggttgtgaa atgattctca aatgaatata
    18061 atagaaagaa aaaggaggaa gaatcaatga aaatagcaat tgcaggcgct ggcgcaatgg
    18121 gaagtcgctt tgggttaatg cttcaccaaa gtgggaacga agtattatta atcgatggct
    18181 gggcagaaca tgtccaacaa atcaaagagc atggattaca agcaaatttt aatggtaaag
    18241 aggtagaagc aaaactacca atcgttcttc aatccgaagt agaaaaagaa gatcaagttg
    18301 atctgattat tctatttacc aaagcgatgc agctggaaaa aatgctgcag gatatccaat
    18361 cattaatcaa aaaagataca gaggtcttat gtctattaaa tggtatcggg catgaagata
    18421 ttattgagaa atttgtacca atggaaaata tctatattgg aaataccatg tggacggcag
    18481 gtcttgaagg tcctggtcag gtcaaattat ttggaagtgg ttcagtagaa ttacaaaatt
    18541 taggtgatgg aaaagaagca gctgcaaaaa aattagcaga caaattgtct gaatcaggat
    18601 taaatgctca tttttctgac aatattcact attctattta tcgcaaagca tgtgtgaacg
    18661 gaacaatgaa cggactatgc acaatcttag acgttaatat ggctgagcta ggaaaaacat
    18721 cgactgctca taaaatggtg gcgacgattg tcaatgagtt tgccaaagta gcagcagtag
    18781 agaagattga actagatgtc ccagaagtca ttgcacattg tgaatcttgt tttgacccgg
    18841 aaacaattgg gttacattat ccttcaatgt atcaagactt gattaagaac catcgattaa
    18901 cagaaatcga ttatatcaat ggcgcaattt ctagaaaagg gaagaaatat ggtgttgcga
    18961 cgccttattg tgatttcttg actgagcttg tccatgcaaa agaagatagt ttgaacgtaa
    19021 aataaaggag agattgaatg gaaacttcac aaaaaatatc accgaaaatt tttctgaata
    19081 aagtcctcgc tggtacagca acaggaatca ttattggatt gattcctaat gcagtattag
    19141 gtgccatttt aaaatatttt agtgcgtatc cctttgcggt aacgatcgca cagttagcag
    19201 ttatttttca actagcaaca cctttgatca ttggtggttt gattgcctta caatttggat
    19261 ttgatccgat gagaatgatg gttgtagccg gagcttcttt tgttggttct ggtgtagtca
    19321 aattcacacc tgatttgggt gaaacgggtg tttacgttgg tgctggtaca ggcgatatta
    19381 ttaatacgat gctgacagca tcgattgctg tcgggctgat tttactgatt ggaaatagtt
    19441 ttgggtcagt gtcgattgtg ttaacaccga ttgttgtagg tgtaggtgca ggtttacttg
    19501 gtttttattt attccctttt gtaacagcaa ttacttctgc aattggatca ttgattaata
    19561 acttcactac cttacaaccg ctgttaatgt ctatcttgat tggttgttcc tttgcctttt
    19621 tgattatctc tcctatttca acagtagcaa tcggaatggc aatccaacta gatggtgttt
    19681 ctgctggtgc ggctgcgatg ggcgtagctg caaccacttt tgttttagta gtgaactctt
    19741 ggaaggttaa taagtcaggt gtgacaattg cgattgcttt aggcgctatg aagatgatga
    19801 tgcctaatct atttagaaaa cctgtgattc ttattccttg tttagttagt gcagtgatta
    19861 cagctattcc tgtcgctcta ttttctattt caggtacgcc tgcttctgct ggtttcggcg
    19921 tggtcggact tgttggtccg ttggcttctt tagatgcagg gctaaatggc tttcttgttg
    19981 ttctttgttg gttagtcatc cctgtttttg cggcactaat tactcaattt ttatgcgaga
    20041 aagtatttaa actgtatgat cgagaagaag tcttcaaatt tttaggctag tagattttac
    20101 taagaaattt tcaatcaatg gatgcgtaag tgagatgtca acaacatctg acttacgttt
    20161 ttttgccttt ttgagtagga gattgatgct gacaactgaa ctcttcagtg ccattcgatt
    20221 ttttaaaatg tagaagaaac tcacctttat gttattgatt gttcaaaagg caactagaat
    20281 aaaaatatgt gttaaaatga gaaaaaattt gattgttgga tgtatcgaat aatcactaaa
    20341 aagtaggaga agaaaatgat ttttttgcaa aaatatggtt tttatttttt attattaggt
    20401 gtgattagtg atttatcgac accgtatatc cttggccttt tttatcccaa actgaatcaa
    20461 atgactaccg taattagtgt gtttggtgac gtagatagtc cagtcagacg tgctttcttg
    20521 gtctggtcag ttgtatctgg actttttttt gttctttcgt tgcctgcgtt gtatcaccta
    20581 tttattggaa catcaaaaac acttgcaata cttgctgttg caacagttgg tttatatggg
    20641 ataggtgatt gtatttttac tgggttattc agtatcaata cgaatgaaag ctcttggaat
    20701 ctatcgactt ggatccataa tataggttct gggctaggct atgcaggctt tctacttttc
    20761 ccattgttac ttgttctgct ttatcgtcaa agtggtcaag gtacgcttag ccatttttat
    20821 cttgttttaa cagttattag tttactaatt gcaggtcttt acggattagc acggattccg
    20881 tcgattagtc agtttgcctt ctttaagcaa ttgggcttct ttcaacgctt aagttttttc
    20941 tttaactatt tgccgatgat ttgtttaagt ttttttcaac tacgaaagta gaaaaagcag
    21001 tgtgaagctg attaagtaaa agaatttctc tatgggtaac cattcgtgtg gatgtgttcg
    21061 actaagaata gagatgagga gagcgaaaag caagttagtc ttaacttgtt atgcgttgtg
    21121 ctaaatttca ggtagtcatt ttttgaatga ataagaagaa aacttgaaag agggtgactt
    21181 catgttagat gtggagcaga tggaaataga cctttataat attattttaa atcgaaatac
    21241 aagagaatgg gaaagaacac aatttgaaga ggcaaaagaa gacctagcaa aaatgtcgat
    21301 aaagcaggtg atagaaaaac tagaggtcaa cttgcgtccg ctagcactac gagataattt
    21361 gacaccagat gcgatggatt tttatttaag aatcacaggg gagtcagcga gtgcaaaggc
    21421 ttatgattat gccaaacata agataacttc acccttatac caagaacggg cgatttttgc
    21481 cggtggctgt ttctggtgta tggttgagcc ttttgaaaca aaaccaggtg taatctcagt
    21541 gttatctggt tatacaggtg gagagaccaa aaaaccaaat tacgatcaag tcagtggtgg
    21601 atatactggg catgttgaag ctgttgagat catatttgat acacgtcaaa taacctatga
    21661 agagctgttg gagatttatt ggcaaatttc agaccctaca gatgcctttg gtcaatttca
    21721 agatcggggg aaacaatatc gttcaattct atttgttgtt aatgaaaagc aaaggcaatt
    21781 ggctgaaaaa tcaagagaac aactgattca atctaaccga tttagccgtc ctatcgttac
    21841 agagattcgc caagcaaaga ctttttggcc agctgaaaat catcatcaac aattttatca
    21901 gaagcaacct aaaagatata aaaagatcaa gcattcgcat cagcttcatt tattttttca
    21961 gcgacataga tagaggaagg ttgtggcgca aagcgttcag ctccgacaaa ataatgagca
    22021 actgacaaaa atagcttttc atatttttgg acagttatga attatttcga ggagttgctt
    22081 tgagaacacc gaggaaaagg ttgtggcgca aagcattcag cttcaagtcg caaggaaaca
    22141 ggaggtatga caagtctttt gtcacaactc ctgttttttt actagccttt atttccatgt
    22201 gaaagagaat tcgaattgta gaatcttgat ttttctatgg aattttgtag agaatcatct
    22261 tgataattaa tagacaccta atcgaattcc acattaatga tgaactaaat tccgtataaa
    22321 agcgcaaaca ataacta
//
LOCUS       DACEJR010000046        21511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEJR010000046 DACEJR010000000
VERSION     DACEJR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.046"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IW651_001846"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IW651_001846"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0814567.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IW651_001847"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IW651_001847"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0814568.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IW651_001848"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IW651_001848"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0814569.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IW651_001849"
     CDS             complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IW651_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0814570.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3503..4483)
                     /locus_tag="IW651_001850"
     CDS             complement(3503..4483)
                     /locus_tag="IW651_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0814571.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4507..5991)
                     /gene="galT"
                     /locus_tag="IW651_001851"
     CDS             complement(4507..5991)
                     /gene="galT"
                     /locus_tag="IW651_001851"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0814572.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6003..6992)
                     /gene="galE"
                     /locus_tag="IW651_001852"
     CDS             complement(6003..6992)
                     /gene="galE"
                     /locus_tag="IW651_001852"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0814573.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7240..7407
                     /locus_tag="IW651_001853"
     CDS             7240..7407
                     /locus_tag="IW651_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0814574.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7469..9280)
                     /locus_tag="IW651_001854"
     CDS             complement(7469..9280)
                     /locus_tag="IW651_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0814575.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9277..9642)
                     /locus_tag="IW651_001855"
     CDS             complement(9277..9642)
                     /locus_tag="IW651_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0814576.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9805..10200)
                     /locus_tag="IW651_001856"
     CDS             complement(9805..10200)
                     /locus_tag="IW651_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0814577.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10218..11180)
                     /locus_tag="IW651_001857"
     CDS             complement(10218..11180)
                     /locus_tag="IW651_001857"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0814578.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11180..11392)
                     /locus_tag="IW651_001858"
     CDS             complement(11180..11392)
                     /locus_tag="IW651_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0814579.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11413..12111)
                     /locus_tag="IW651_001859"
     CDS             complement(11413..12111)
                     /locus_tag="IW651_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0814580.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12131..12673)
                     /locus_tag="IW651_001860"
     CDS             complement(12131..12673)
                     /locus_tag="IW651_001860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0814581.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12805..13809)
                     /locus_tag="IW651_001861"
     CDS             complement(12805..13809)
                     /locus_tag="IW651_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0814582.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13806..14795)
                     /locus_tag="IW651_001862"
     CDS             complement(13806..14795)
                     /locus_tag="IW651_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0814583.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14792..15598)
                     /locus_tag="IW651_001863"
     CDS             complement(14792..15598)
                     /locus_tag="IW651_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814584.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15764..16720
                     /locus_tag="IW651_001864"
     CDS             15764..16720
                     /locus_tag="IW651_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0814585.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16797..17315)
                     /locus_tag="IW651_001865"
     CDS             complement(16797..17315)
                     /locus_tag="IW651_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0814586.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17403..17552)
                     /gene="rpmG"
                     /locus_tag="IW651_001866"
     CDS             complement(17403..17552)
                     /gene="rpmG"
                     /locus_tag="IW651_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0814587.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17780..18226
                     /locus_tag="IW651_001867"
     CDS             17780..18226
                     /locus_tag="IW651_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0814588.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(18421..20316)
                     /gene="asnB"
                     /locus_tag="IW651_001868"
     CDS             complement(18421..20316)
                     /gene="asnB"
                     /locus_tag="IW651_001868"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0814589.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(20641..>21511)
                     /locus_tag="IW651_001869"
     CDS             complement(20641..>21511)
                     /locus_tag="IW651_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0814590.1"
                     /translation="RFDFRKVKNLDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLN
                     FPGNADYKELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATINLVNIDFSEKLPLSP
                     LHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENH
                     FSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNAASGDSESESISQFFHILGS
                     VEQQKGVCDTGERKYEYTIYSSCCNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVS
                     YPVIKEQQINYVN"
BASE COUNT         6436 a         4575 c         3689 g         6811 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacac ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcactccat
     3481 tttcccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
     3541 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
     3601 ctagctctcc catccattcg gtataaactt ttacagtaga aagcgagggc gttacatatt
     3661 tcgctacact gacatcattg aagcccatca cagaaatatc ttctggtacc actaaacctt
     3721 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
     3781 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
     3841 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
     3901 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
     3961 caagcatagc gatatgttgc gctttatggg agacaaagtg ttcaatgact ttttgaacac
     4021 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
     4081 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
     4141 gtgcgatcgt tccatctgcc ggaagatcac taatggaatc ccacgtttct ttcaataaag
     4201 taacgtttgc ttcttctgcc tttttttcga ttcctaggcg aatagataaa taataaagat
     4261 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
     4321 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
     4381 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
     4441 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
     4501 ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
     4561 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
     4621 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
     4681 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
     4741 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
     4801 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
     4861 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
     4921 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
     4981 ttcatccgaa tatgctttcc atttttccaa tactgagtta gctgcttcaa taagcaagtt
     5041 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
     5101 ttgcggataa tcttctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
     5161 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa ttggtaagtc
     5221 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
     5281 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
     5341 gtaataagca taaggggaat attggaatcc ccagctttca ccgtctagat tcattcgaat
     5401 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
     5461 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
     5521 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
     5581 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
     5641 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa
     5701 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
     5761 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
     5821 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
     5881 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
     5941 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
     6001 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatc
     6061 gctttgatat ctgtgtattc aggtttccag cccagaatct ctctcgcttt ttcacttgaa
     6121 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
     6181 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
     6241 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
     6301 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
     6361 tcgtctccat aaacagccaa ggcttttctt tgaccaagag ctacttgtaa aatgattggg
     6421 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
     6481 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
     6541 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
     6601 tctgtgattg gagactcttt cggctctcca taagttgctg cagtagaaga gaatacgatt
     6661 ttattcacgt tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
     6721 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
     6781 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
     6841 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
     6901 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
     6961 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
     7021 gtgtttgttg attctatata atttaaaaaa cgaagtttac ttttatggtg aaaacaaaca
     7081 aagtaaaatt tcgtaaattc atatggtaaa tttataataa aacatttagt aaacattttc
     7141 aaggattttt tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt
     7201 ccataataga tataacaaat aataaatgag gtggattcaa tgaaaaaatt tgtatctgga
     7261 atattagtag gaagtctggc aacagccgct gccgttgctg gtttggttgc ttctgtgaaa
     7321 aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa
     7381 gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa atacgcatga agactttcta
     7441 aaaaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt tgtgctattt
     7501 tatctgcctg tctcagattc catggatctg tataaggtct agctactgct gcagttagcc
     7561 aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt tcccctgaag
     7621 catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga taacttcctg
     7681 tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt tttatgacag
     7741 gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc
     7801 tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga atatcaaatg
     7861 gcggttcgat caaatgaaaa atacccgcat ctatcagtgc tgctaattct ttcgtccgaa
     7921 tgattggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc catagtattt
     7981 ctttgtattc cttagcagaa aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc
     8041 agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct ttttccgctt
     8101 cttttatctg cccttcaata aatattcggc tagcttctga aaagggctca ttctgatagg
     8161 caagaggagt ttctaaatac gaccaatccc atctataagg gattagtgct ggatgctttc
     8221 cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc tttggaatcg
     8281 gcaagtgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct tttttcagat
     8341 agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc aggttttctt
     8401 ttgttagtaa ccgtggccat gccattgctc cgccttgctt ttgatttctt cctcgagggt
     8461 aataagggag cccccttctt gatccactgt atacaatcgg ttccttacct gatggatgat
     8521 agaccagttt tcccttttcg ttctcgaagc gccctcctct tgatactgta aacaatccga
     8581 cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta ggcggtatct
     8641 tcgaaagatc tacatctgca ggattagaag gaagctgata cagtaaaaca tttttctttg
     8701 cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga ccgcttgcta
     8761 aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa ccatcttttc
     8821 ttttgcttac tttttttact aaagaataaa caaactgaat agaagctgga aattcttctt
     8881 gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta gattgctgat
     8941 ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt accgtctgtt
     9001 cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc tgtgttacca
     9061 atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa acagaattca
     9121 ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca ttgggatcga
     9181 aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt tctgctacac
     9241 ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct ccatttcgat
     9301 aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat aaacaaaagt
     9361 aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct tcttttcttc
     9421 ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg
     9481 gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca gatctaactg
     9541 cgctttttct tttgaaattc ctcgtggcca gaatctatcc acgagtattc tatagccatc
     9601 ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt ccttctttct
     9661 accttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa tgaacggcac
     9721 aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga ttcggttttt
     9781 aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc cagaccatcc
     9841 gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta atttatttgc
     9901 tgtacggatg ctcaaacttt tcttttgatc gtaaatatag acaggctggt cttttcgaat
     9961 agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta aaatatgccc
    10021 agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca ttccttctcg
    10081 aaattcttct tctgtcaaag tcgttgctga acgtttggcc attactcgta gatagacttc
    10141 ccaaaggatg attgcaagca caatcaatag caaaatgata ttaattaccc ataaaaccat
    10201 gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc aatgacacca
    10261 gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg cgggaaagta
    10321 aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc cgatactcct
    10381 ccacctaaca cgatacttga cggatttaat gtattcccca agtttccgca agctaaacct
    10441 aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt
    10501 tcaaagatat ctttacttgt tacttcttct ccgttgtcta gcattgcttt cagttttgaa
    10561 tcccctgcgt attcttctgc caagaaacga cctaggcgta ccacacctgt agcgctagat
    10621 acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg atccacagtg
    10681 atatgtccga tttctcctcc agctccgctc acgccgtgaa tcaaatttcc gccagcaacg
    10741 atacctccgc caacacctgt tcctagagta acgaatacaa cgtctggatc gttttctcca
    10801 gcacctttcc atcgttcacc taaagcagca acattcgcgt cattatcgat agcgaatttg
    10861 attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca gtttaagtta
    10921 tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc catgccgatt
    10981 ccgataaagt cttcggcttt catattatat aaattcaaac gatgattgat tgattcaatg
    11041 atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct ccatttttgc
    11101 tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc taaatcaatc
    11161 ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca attcggtgct
    11221 ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa cggctatcat
    11281 acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca cccacataga
    11341 taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc atctttaagc
    11401 acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa tacatattac
    11461 aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg
    11521 ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc caccgacatg
    11581 tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca caataaataa
    11641 aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa accctaagat
    11701 aaacaacgca ccgaacaaac cgaatatcga agtacttgca ccaccagaca gaacattcgc
    11761 ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat aaatcaagaa
    11821 aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca gtatcagtga
    11881 gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta aacgccacca
    11941 ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga tctcaagtcg
    12001 cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca
    12061 taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca tgaaaacctc
    12121 ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc tccggttgcc
    12181 attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga aaattattta
    12241 aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta ttgtagataa
    12301 tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct gattgtggct
    12361 caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca taaaacgcca
    12421 tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt tctgctcgtt
    12481 ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct atcacttttg
    12541 ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct ttttcctttt
    12601 tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt aatcttactt
    12661 tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga aaaaaagaaa
    12721 aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac ctagaaatga
    12781 ttggccttcc ttttttaatg aatttcaact gttcagcgcg tgtttttgtc tgataaaaag
    12841 tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag gtacttcccc
    12901 acttagtaga atcactctag caaccatatc tgcactaagc agaaaaatgg aaccgaacaa
    12961 accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg cagcatgagg
    13021 agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc ttgccaaagc
    13081 aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta atccttgcgc
    13141 gcgttcttga ccaaatgata gcaatacaat cctctgtgca taaagtcctc ctagcggaat
    13201 taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac cccagattga
    13261 tcccgctaac catgcattca caaatccata attttcctta gataattgga tcgtgcatag
    13321 caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga ttcgattcat
    13381 acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag ctaaaacacc
    13441 acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa
    13501 ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag aatctgccaa
    13561 ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag ctcctgctaa
    13621 taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca aagaagatga
    13681 ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg taccaatcat
    13741 taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg tccaatatat
    13801 ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc aatcaatgca
    13861 atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc
    13921 aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg atagtccgtt
    13981 ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac aggtccgact
    14041 aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa ccgcgttcgt
    14101 ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa agatattgct
    14161 tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa tactgctaat
    14221 tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc ctgctgcaga
    14281 ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat agctacgcca
    14341 gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa accaaatacg
    14401 agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa atcagaagct
    14461 ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt gattcctagt
    14521 aagcctgaat ctgctaaagg atttcttgtc actccttgca tcaatgcacc gctcaatgca
    14581 aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt tcgtatgatt
    14641 tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc tgcttttccc
    14701 acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc
    14761 aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc tcttttggca
    14821 acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca atcgttgcat
    14881 caatctcaaa tatttttttc atacactctt ttgtgatcac ttcttcaggt gtaccttctg
    14941 tgatcaactt accgtttttc attgctaata aataatctga aaaacgtgaa gcatgattaa
    15001 tgtcatgaat cgacatcaag atcgttgttt gtttcatttg attgatttca gtcaataact
    15061 gtaggatctc taattggtgt gccggatcta aaaatgttgt cggttcatcc aagatcaaga
    15121 tatctgtttc ctgagcaagc gccatagcga tccagacacg ctgggtttgt ccaccagaca
    15181 gcgttgcgat atcccgcata cgaaatccag ctagacctgt agcatccaac gcccactcaa
    15241 ttgcttcatg atcttgcgtg tccatgccac tgaacagttt ttgataaggg tatcgtccgt
    15301 aagatactac ttcttctact gtcatacctg tagggtgttc actagtttgt gctaataatg
    15361 caattttttt tgcaagttct tttgaactgt atgtagagat atcagagtta ttgacaatga
    15421 tctttcctga tttcacaggt agtatacggg taaaactttt caataaagta gattttccac
    15481 ttccatttgg cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca gacatgtttt
    15541 caatgatttt tttgttttcg tagccggctg caatgtcagc tgctcttaat ctcgtcattt
    15601 cacccatcct ttcgctgaaa atcattctca attatgacgc tgtttcaaca tttcgtcaag
    15661 cgctactttc atatctatag ccaaatgaag cggttgacag cgttcgaaat atcaggaata
    15721 ataaccaatg agaaagattc tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa
    15781 agtattcaca ttatctagca tattgctgct ggctggagta ctgagcgctt gctccccctc
    15841 ttcagataaa gaaacagcta caactgccaa ttcgacgact tcatccaata gtactcatac
    15901 agtcacagat acacttggac ataaagtaga gattccaaac gcgcccaaac ggatcatcgg
    15961 cagttactta gaagactatc tgattgcact aggggaaaaa cctatcgccc agtggactgt
    16021 tggcagtggt tccattcaag attatctgca agatgattta aaggatgtcc caacgatttc
    16081 ttatgacctt ccttatgaga aagtgttaag ctttgagcct gatttgctgt tgatttcttc
    16141 ttccgctact gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc caacttatgt
    16201 tgtaaaaaac ggaacagatg tcacatggga agaacaacta aaagatgtcg gcaaagcttt
    16261 ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat caaaaatcca tcaagaaaac
    16321 tcgagaagaa ctagcagata aaatcgagaa caagacagct gcagtgttgt gggtgaccaa
    16381 caactcagcc tttatggtca gaaaaaatcg gtcaagtgga agaatcgtat acgacgatct
    16441 ggaatttggt gttcctgatc tagtggaaga ggtctccaaa aaagccactt ctgactggtc
    16501 tgctgtttcc tctgaaaagc tagctgaatt agatgctgac tacatcatcc ttgtgaacag
    16561 tgacaaagat gctgccatgt tcaatgaacc aagctggaag aatctaaaag cagtgacaga
    16621 caatcatgta ctgcaattcg gacctgaatc tagttggcta tataacggac ctattgccag
    16681 tcaggaaatg gtcaatgata tcaaagaaaa actaaattga ttttaactaa aaaaagcatc
    16741 ttcacgatta gtcgtgagat gctttttttc ttattaacaa actagacgca tagattttat
    16801 tcttcttttg aagcaaccct attttttact ctttcccatt tttgaaaacg atgggcatat
    16861 agattttctt gatccgttat cccctcatac tcctctataa gggcgaagtc tgaaaaatca
    16921 atattcccaa tatacgtatc gccttcaaac gtgctatcga tcaatgttcg ccagatccga
    16981 ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac ccgaaacata gatatcttct
    17041 gagatcgttt gactatacga caaaagttca tcgatcgaat gcattacttc cgcattttct
    17101 ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat ggcgataagg aagtgaaaga
    17161 tcccccattc cttcataggt ctttcttccc atcactaaaa ttcgatccat tgtgtgttcg
    17221 cgaaagaaac gcatatcatt tggcaatcgc catggtaaca aaccattttt gccgatcaga
    17281 ccatttttat cttgtgccca catggaaata aacatattat ccgctcctta tgattttaga
    17341 tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga actttaaaaa
    17401 cattattttg tttcacggtg caaagtaact ttgcgttcgc gtgggcaata tttttgtttt
    17461 tccaaacgat caggattgtt acgtttattt ttgcttgtta gatagttacg ttctttacaa
    17521 gaagtacact ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt tacaagaatc
    17581 ctctaagatg actctcagac ttgattatct taccatgatt ttttctagat tgctagtgtt
    17641 tttcacgatt ttgttaagta aaactacttg acgataaata caacggtttt acttctcaaa
    17701 caaaatcttt ttctttttgt ataaataccc ttacacttgt ttatatagat tatgtttttt
    17761 aataaagggg gatactaata tgccatggaa catgaaggac tttcctgcat caatgaaaaa
    17821 cttagataaa ttgacaagaa aaaaagcaat cgacatcgca aatgcattat tagatgaagg
    17881 gtaccctgat aaccgtgcga tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga
    17941 tgctagtgaa tcagaaaaac gaactttcga aaaagaaaaa aatccaagca agacggatga
    18001 acacgatacg aatcctcgtg ctggaaaact gcttgattca gatgtgatcg tagaatatga
    18061 ggaagaacaa tggatcgtga aatctaaagg ggcaaaaaag gcaagcaacc attttgatac
    18121 aaaaaaagaa gcaatcgaaa aaggaaagca agtagcacaa aacaaagaat caactttgtt
    18181 catttataaa aaagatggga caaaagaaaa agaaatcgat tattgaacat atgtaaaggg
    18241 gttgtgacga aaatcgtgtc acaaccccct tatgttcgaa acaatggtgt tcctctgcca
    18301 actccgaacc ataagtcata atatctataa aacgggaaga gctgtttttt gaaattgttt
    18361 cttatcactc agtgttaaac ggtaggagaa aacctctttt ttattgatat ttgattttca
    18421 tcaaacagtc acatagtcga ttgcttctgc ttgcggaatc gtttcatcta caaagtagac
    18481 tttataccat gtcaagaaac tgaagattgt ccagattttt ctttgctctt ctttacgtcc
    18541 ttcgtgatgg tcatctagca acttcatgat tttttcttga tcaaaaaatt cttttgcaaa
    18601 atctgcttcg aataatgctt tgacttgttc gtagccgtgt tcttctttca accaagcttt
    18661 gattggcaca gggaatccta atttcacacg attcgaccat tcttccggaa gatgtttgtt
    18721 cgcagcttgt ctgaaaatat cttttgtgtt gttttgattc aataaaaact tacttggtat
    18781 ttgttgagcc aatttcatga cttcgatatc caataaaggt actcggactt ccaaagaact
    18841 tgccatagaa agtttatctg cctttaacaa gatatctttt ggcatccatt gatgaagatc
    18901 gacatattgc atcttattga cttcatcttt gattccttct gttttctcgt aatgaacagt
    18961 catgatttca tccacagatg gtgctgtttg atattttggt gttaacactt ctgatgcttc
    19021 tgattcttcg aacactcgag catgaccgat aaaatattct tttgcaggag caagtgattc
    19081 atataaatgg atccgtccat ggaaattttt catttttccg attttacgtc ctaaattata
    19141 gcgtgttcct tttggtaatt ttttcaatcc ttgagcaatc acacggatga attttgaatt
    19201 cgtatggaat ccatacgttt ggtatcccgc aaacagttca tccgcccctt ctccagattg
    19261 gactacccgc acactttgtg aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg
    19321 atttgaatca ggttcatcta agtgatactg gatcaaaggg aatgctttga acgacatgtc
    19381 tccgtcgatc actgctgctg tattatctaa gtccaatagt tcggttagtt ttctggcttc
    19441 aatcgcttca ttataggttt tatcatcaaa cccgattgag aaagaatgat ctggtttcaa
    19501 tacagaggtt acataactgg aatcaacccc gctggataag aaagaaccaa cttctacatc
    19561 actgaccgta tgtgcttcaa tagaagcttg aacggtttcg tcgatttttt cgatccattc
    19621 ttgtcgggaa tgcgtttctt ttgtttcaaa atctgcatcc caatattgct gaatgtctat
    19681 ttttccatct ttatacgtat aataatggcc ttcaggcaaa cgatagacat ctttgaagaa
    19741 tgtttcgccg tttaatggag aatattggaa agtcatataa ggtttcaacg cttctttgtt
    19801 caattctttg ttaaaatcag gatgaggcag aaaacttttg atttctgaac caaacataaa
    19861 tgttccgttc atttctgcat aataatatgg tttgatccca aagtggtctc ttgcaccgaa
    19921 taactcattc ttttcattat cccaaatagc aaaagcaaac attccgcgga tcttttgcaa
    19981 taattctgtt ccccattctt cgtaaccatg caaaaggact tctgtatctg catgtgtttg
    20041 aaaaacgtgg ccagctgcaa tcaattcttc tctcaaaggc tggtaattat agatttcgcc
    20101 attaaaaata atgatctttg tccgatcttc attgtaaata ggctgagtac cgccctctaa
    20161 gtcaataata cttaatcttc tgaatcctaa agcaacatgt ttatcgatat attctcctga
    20221 actattaggt ccacggtgaa cgattcgatc catcattgta ttaataattg tttttttgtt
    20281 gtccttgtcg ttaacaaaac ctacaatacc gcacatttaa ctttcacatc ctaatataaa
    20341 attactgttt atttcattca tatgcatctt atcatagaat aaatagcgga cgaatatttt
    20401 ctctcttaca atttcgtcac gtgaaaacaa aagatgaaag tttctgtttt tacagaaaat
    20461 aaagtcccaa tatctaattt accatgtttt cttttttgag gctaccttat tctcttcgtg
    20521 ttaaaaaaat attagtggat gctatttcca aaaaaaccag caaaaaaaga cacgacctaa
    20581 agatcgtgtc taattatgac tgtatcagaa aatatgatac caaacggatc tattcattaa
    20641 ttaattgaca tagtttatct gttgctcttt tatgactgga taactgacca attcatgacg
    20701 atccaagtct tctttattca tatttacagc agtaatttga ctgtcttcat acgtacgata
    20761 ataataaatc cctttatcaa cgttacagca agaagaataa atcgtatatt cgtattttct
    20821 ttctccagta tcgcatactc ctttttgttg ctcgactgag cctagtatat gaaaaaattg
    20881 actgatactt tcagattctg aatcacctga tgcagcattc attttagtaa atgtggcttt
    20941 tacaaatcga gaaacagaag agagatctcc aggtaagccc aatcctccca tcccacgact
    21001 ataaacattc aagctgatct ggtttgaaaa atgattttct ggtgttccat ttgatagcga
    21061 gcgataattg ttcagattaa ataattgata atcaaatggc ggattatttg tcaacacacc
    21121 gacaggatta tcatataaat gaagtccatc tttcatactt tcaattacta tcgatttttc
    21181 tttatcagct aataaccagt gtaaaggtga caagggtagt ttttcactaa aatcgatatt
    21241 gactagattg atagtagcca ataattcttt agcttcgtct atggttgagc attgccctag
    21301 tatccatgga ataaactcaa atggtgcaac gttatctttt ccttcttgta attctttata
    21361 atcagcattg ccaggaaaat tcagtcctgc catactcaac cctttttcat ttgtagcctc
    21421 ataatagagt ggatagttgg ataccccagc tgctattcct atcattgcat aatgtttatc
    21481 taaatttttt acctttctaa aatcgaaacg a
//
LOCUS       DACEJR010000047        21504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEJR010000047 DACEJR010000000
VERSION     DACEJR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.047"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW651_001870"
     CDS             5..403
                     /locus_tag="IW651_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0814591.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW651_001871"
     CDS             387..1082
                     /locus_tag="IW651_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0814592.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IW651_001872"
     CDS             complement(1666..2553)
                     /locus_tag="IW651_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0814593.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IW651_001873"
     CDS             2738..3577
                     /locus_tag="IW651_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0814594.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="IW651_001874"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="IW651_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0814595.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="IW651_001875"
     CDS             5045..5401
                     /locus_tag="IW651_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814596.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="IW651_001876"
     CDS             5404..6561
                     /locus_tag="IW651_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814597.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="IW651_001877"
     CDS             complement(6587..7027)
                     /locus_tag="IW651_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0814598.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="IW651_001878"
     CDS             7187..7693
                     /locus_tag="IW651_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0814599.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="IW651_001879"
     CDS             7861..9774
                     /locus_tag="IW651_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0814600.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="IW651_001880"
     CDS             9779..10780
                     /locus_tag="IW651_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0814601.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="IW651_001881"
     CDS             complement(10982..11188)
                     /locus_tag="IW651_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0814602.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="IW651_001882"
     CDS             complement(11834..12682)
                     /locus_tag="IW651_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0814603.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12846..13748
                     /gene="gnd"
                     /locus_tag="IW651_001883"
     CDS             12846..13748
                     /gene="gnd"
                     /locus_tag="IW651_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0814604.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="IW651_001884"
     CDS             13750..14751
                     /locus_tag="IW651_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0814605.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="IW651_001885"
     CDS             14748..15032
                     /locus_tag="IW651_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0814606.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="IW651_001886"
     CDS             15065..15862
                     /locus_tag="IW651_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0814607.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="IW651_001887"
     CDS             15876..16709
                     /locus_tag="IW651_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0814608.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="IW651_001888"
     CDS             16727..17584
                     /locus_tag="IW651_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0814609.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="IW651_001889"
     CDS             17606..18115
                     /locus_tag="IW651_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0814610.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="IW651_001890"
     CDS             18152..18553
                     /locus_tag="IW651_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814611.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="IW651_001891"
     CDS             18665..19018
                     /locus_tag="IW651_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814612.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="IW651_001892"
     CDS             19052..19495
                     /locus_tag="IW651_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0814613.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="IW651_001893"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="IW651_001893"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0814614.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="IW651_001894"
     CDS             20590..21303
                     /locus_tag="IW651_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0814615.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7400 a         3302 c         4491 g         6311 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttcc
//
LOCUS       DACEJR010000048        21244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEJR010000048 DACEJR010000000
VERSION     DACEJR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.048"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW651_001895"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW651_001895"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0814616.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW651_001896"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW651_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0814617.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW651_001897"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW651_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0814618.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW651_001898"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW651_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0814619.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW651_001899"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW651_001899"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0814620.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW651_001900"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW651_001900"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0814621.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IW651_001901"
     CDS             complement(8054..8524)
                     /locus_tag="IW651_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0814622.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IW651_001902"
     CDS             complement(8570..8995)
                     /locus_tag="IW651_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0814623.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IW651_001903"
     CDS             complement(9062..9331)
                     /locus_tag="IW651_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0814624.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IW651_001904"
     CDS             complement(9342..10937)
                     /locus_tag="IW651_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0814625.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW651_001905"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW651_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0814626.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGIDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IW651_001906"
     CDS             complement(14490..15050)
                     /locus_tag="IW651_001906"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0814627.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IW651_001907"
     CDS             15403..16377
                     /locus_tag="IW651_001907"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0814628.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IW651_001908"
     CDS             16547..17947
                     /locus_tag="IW651_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0814629.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IW651_001909"
     CDS             complement(18025..18747)
                     /locus_tag="IW651_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0814630.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IW651_001910"
     CDS             complement(18826..19332)
                     /locus_tag="IW651_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0814631.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..>21244
                     /locus_tag="IW651_001911"
     CDS             19455..>21244
                     /locus_tag="IW651_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0814632.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTE"
BASE COUNT         5903 a         4660 c         3834 g         6847 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctattc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aaga
//
LOCUS       DACEJR010000049        21114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEJR010000049 DACEJR010000000
VERSION     DACEJR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.049"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            474..725
                     /locus_tag="IW651_001912"
     CDS             474..725
                     /locus_tag="IW651_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814633.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IW651_001913"
     CDS             759..1037
                     /locus_tag="IW651_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814634.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IW651_001914"
     CDS             1634..1891
                     /locus_tag="IW651_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814635.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IW651_001915"
     CDS             2004..2615
                     /locus_tag="IW651_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814636.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IW651_001916"
     CDS             2794..3351
                     /locus_tag="IW651_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814637.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IW651_001917"
     CDS             complement(4050..4571)
                     /locus_tag="IW651_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0814638.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IW651_001918"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IW651_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0814639.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="IW651_001919"
     CDS             8651..9538
                     /locus_tag="IW651_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0814640.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="IW651_001920"
     CDS             9535..10704
                     /locus_tag="IW651_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814641.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="IW651_001921"
     CDS             10728..12083
                     /locus_tag="IW651_001921"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0814642.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IW651_001922"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IW651_001922"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0814643.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="IW651_001923"
     CDS             14369..14659
                     /locus_tag="IW651_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814644.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="IW651_001924"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="IW651_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0814645.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="IW651_001925"
     CDS             15700..16359
                     /locus_tag="IW651_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0814646.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="IW651_001926"
     CDS             16516..17448
                     /locus_tag="IW651_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814647.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="IW651_001927"
     CDS             17602..18891
                     /locus_tag="IW651_001927"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0814648.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="IW651_001928"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="IW651_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0814649.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="IW651_001929"
     CDS             20471..21058
                     /locus_tag="IW651_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0814650.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7025 a         3605 c         4568 g         5916 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcctg ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagt
//
LOCUS       DACEJR010000050        20729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEJR010000050 DACEJR010000000
VERSION     DACEJR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.050"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..294
                     /locus_tag="IW651_001930"
     CDS             <1..294
                     /locus_tag="IW651_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0814651.1"
                     /translation="IITDMPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISR
                     QILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(752..1027)
                     /locus_tag="IW651_001931"
     CDS             complement(752..1027)
                     /locus_tag="IW651_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814652.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1481..1687
                     /locus_tag="IW651_001932"
     CDS             1481..1687
                     /locus_tag="IW651_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814653.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1883..2050
                     /locus_tag="IW651_001933"
     CDS             1883..2050
                     /locus_tag="IW651_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0814654.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            2076..2420
                     /locus_tag="IW651_001934"
     CDS             2076..2420
                     /locus_tag="IW651_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0814655.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2425..2706
                     /locus_tag="IW651_001935"
     CDS             2425..2706
                     /locus_tag="IW651_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814656.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2809..3123
                     /locus_tag="IW651_001936"
     CDS             2809..3123
                     /locus_tag="IW651_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0814657.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3101..4795
                     /locus_tag="IW651_001937"
     CDS             3101..4795
                     /locus_tag="IW651_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0814658.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4815..5993
                     /locus_tag="IW651_001938"
     CDS             4815..5993
                     /locus_tag="IW651_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0814659.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5956..6642
                     /locus_tag="IW651_001939"
     CDS             5956..6642
                     /locus_tag="IW651_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0814660.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6642..7802
                     /locus_tag="IW651_001940"
     CDS             6642..7802
                     /locus_tag="IW651_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0814661.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7812..8687
                     /locus_tag="IW651_001941"
     CDS             7812..8687
                     /locus_tag="IW651_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814662.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8684..8995
                     /locus_tag="IW651_001942"
     CDS             8684..8995
                     /locus_tag="IW651_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814663.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8985..9338
                     /locus_tag="IW651_001943"
     CDS             8985..9338
                     /locus_tag="IW651_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0814664.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9328..9729
                     /locus_tag="IW651_001944"
     CDS             9328..9729
                     /locus_tag="IW651_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814665.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9722..10126
                     /locus_tag="IW651_001945"
     CDS             9722..10126
                     /locus_tag="IW651_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814666.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10138..10746
                     /locus_tag="IW651_001946"
     CDS             10138..10746
                     /locus_tag="IW651_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0814667.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10766..11128
                     /locus_tag="IW651_001947"
     CDS             10766..11128
                     /locus_tag="IW651_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814668.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11131..11313
                     /locus_tag="IW651_001948"
     CDS             11131..11313
                     /locus_tag="IW651_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814669.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11330..14761
                     /locus_tag="IW651_001949"
     CDS             11330..14761
                     /locus_tag="IW651_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0814670.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14812..15549
                     /locus_tag="IW651_001950"
     CDS             14812..15549
                     /locus_tag="IW651_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0814671.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15559..17850
                     /locus_tag="IW651_001951"
     CDS             15559..17850
                     /locus_tag="IW651_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814672.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17874..20000
                     /locus_tag="IW651_001952"
     CDS             17874..20000
                     /locus_tag="IW651_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814673.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            20163..20609
                     /locus_tag="IW651_001953"
     CDS             20163..20609
                     /locus_tag="IW651_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814674.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20611..>20729
                     /locus_tag="IW651_001954"
     CDS             20611..>20729
                     /locus_tag="IW651_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0814675.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIIS"
BASE COUNT         7298 a         3119 c         4340 g         5972 t
ORIGIN      
        1 attataacgg atatgccgaa ggcaccgaag catggcaata aggcagaaga cgcgattatt
       61 cagatgatgg atatagaaac ggagagagat gcgattctag cggctttgat ggctcttagt
      121 ctgattagtc gtcagatact ctactacagc ttctgtgtgc cagatagctt ctcaaactac
      181 agaattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa gtcggaagct
      241 ctaatagagt ttgcagaagc atataaacac ggaaaaataa ttgcttataa ataatttggc
      301 ggttttttgg cggaatgatg gcggttttta gacatttacc agtgttatta tggtagtgtc
      361 gaaagattag gaaacaggat cgacaaaata aacgaaggga ggcaatctcc ctcatcgttt
      421 taaacttctt cttagataga cagcaactaa ttatgaagaa aaggatgtga attcaactcc
      481 tcctaaattc ttcatcggtt gctgtttatt ttgattagac agcagcactc acaaaactaa
      541 acatgttctt gtcgtctttc aactgctgct gtctattaat ttatgtattg gagggaagaa
      601 aaatgaaatt atcagtagaa ggcactccag aagaaataca agaattgctc caagctattg
      661 gtagtagcaa ggagcaaaag gtaagtgttt ctaattcaga tattgacggt atttgcaatt
      721 gtttaaacag agaaaaaata tctaatcctt actaagagaa aatatgttcg ttaatgaatt
      781 tccaattttc ctctgtagct ctaccttgat aatttttttg cagttcacag atgaataagg
      841 aatcgttatt atcaagtact gtatttaatt tttcacacat ttgatttgga ttcaaattag
      901 ttttaactaa ccaaaaagat ttttgtagct ttatatatgc cccaaattct tttattattt
      961 caaatacttt atcatacttt tgccctgggt tattaagatc ataactaatc atgtagggtt
     1021 tatacatgtt gtcttgcaca tccttgtttt atatttcagc ggaccactcg ctgataaata
     1081 aaattatacg cttagtattt attttcacaa tattaattta tcgtaataac ttttgagtaa
     1141 atagaacaaa aaacctgcac tagtttccgc tagtacaggc agtgactata tcggttgata
     1201 atctagcata tattaaaaat aattgcaaga aaagaaattt atagtggttt ataccaaatt
     1261 atcaaagcaa tgttattttg ttgctgtcta ttgtttttta attattcaca cgataactaa
     1321 aggtgggtga agagaaatga ttccattaat aatttcaatt tttgcgctct gtcttaatgt
     1381 ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa gaataatttt
     1441 atagtgtcac tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg
     1501 caaccatgca aggttcgatt ccttgccagc gacattaaat gcctatgacg gttacgacta
     1561 ccgaaaaaag atcgttaaga agctatacgg tgctacgtac ggcaatgtag taagtgtgct
     1621 atctgtacac caccaagctt cggtcactgt ggcggaagta gaagacgcag cggtaaatgg
     1681 cgagtagcct cgtgagagcc tggtaagttc tcgtgagtgg tgcaatccca ctccagcgac
     1741 tttaagcaac cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt
     1801 ttgtgcatcg tgcaagttac tattacatat tagatcactc tttgagtggt ctttttattt
     1861 ttgcacaaag gaggaaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     1921 aatcactgtt cataaaggta atggcgaaaa agtatatgag tatagaagac caataaagag
     1981 cgatacatat aaacgaaagg aaaacaatga agttattcca ttgtatggca aaagaatagc
     2041 taagcattaa ataagattgc gaaaggagac ggaacatgac cgaggaattc tatagatggc
     2101 tattacagtt gacaagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     2161 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     2221 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     2281 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     2341 gcagatatct tacagcatta gataaacaag agaagaagat agaaagcttt gctaacttcg
     2401 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     2461 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     2521 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     2581 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     2641 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     2701 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     2761 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     2821 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     2881 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     2941 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     3001 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     3061 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     3121 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     3181 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     3241 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     3301 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     3361 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     3421 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     3481 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     3541 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     3601 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     3661 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     3721 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     3781 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     3841 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     3901 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     3961 aactattaat tacaatgcag atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     4021 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     4081 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     4141 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     4201 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     4261 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     4321 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     4381 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     4441 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     4501 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     4561 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     4621 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     4681 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     4741 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     4801 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     4861 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     4921 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     4981 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     5041 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     5101 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     5161 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     5221 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     5281 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     5341 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     5401 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     5461 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     5521 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     5581 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     5641 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     5701 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     5761 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     5821 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     5881 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     5941 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     6001 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     6061 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     6121 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     6181 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     6241 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     6301 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     6361 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     6421 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
     6481 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
     6541 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
     6601 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
     6661 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
     6721 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
     6781 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
     6841 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
     6901 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
     6961 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
     7021 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
     7081 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
     7141 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
     7201 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
     7261 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
     7321 ctattacagc tttagatgct gtaactatgg gcggtttgat ggcgcgacta tcaaaattca
     7381 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
     7441 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
     7501 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
     7561 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
     7621 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
     7681 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
     7741 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
     7801 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
     7861 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
     7921 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
     7981 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
     8041 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
     8101 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
     8161 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
     8221 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
     8281 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
     8341 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
     8401 tacaaatctg gacagccgag atgtgggcac agctttggaa catgatgtat ttcaatattg
     8461 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
     8521 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
     8581 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
     8641 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
     8701 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
     8761 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
     8821 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
     8881 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
     8941 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
     9001 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
     9061 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
     9121 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
     9181 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
     9241 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
     9301 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
     9361 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
     9421 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
     9481 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
     9541 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
     9601 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
     9661 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
     9721 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
     9781 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
     9841 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
     9901 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
     9961 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    10021 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    10081 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    10141 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    10201 aattttaaga acgcaggaaa tgcaatcgca acgaattgta acggacaatt agatggcgaa
    10261 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    10321 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    10381 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    10441 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    10501 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    10561 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    10621 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    10681 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    10741 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    10801 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    10861 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    10921 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    10981 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    11041 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    11101 aaataaggaa aaaaagcttc aaaagtaaaa attccaaagc tcttagttga aacgtgggtc
    11161 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    11221 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    11281 aactatgcaa taaaccaatc ccagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    11341 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    11401 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    11461 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    11521 gtatgaatta caaagtcaaa aagttgaagt aactagccaa cgtttagcca atgcgaaaaa
    11581 atattatgga gaaaattcca ccgaagttaa gaaacttgaa agagaactga ttaatcaaca
    11641 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatgcac tagctcaagc
    11701 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagcg aacaaaaaaa
    11761 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag caactgctgg
    11821 tcaatcagct tctgaagccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    11881 tgaaaatgcg gagaaaacga tagatatcct aagacgacag ttagaagcta cacagtctga
    11941 atttggcgct acatccacag aagcaatgca gatggaagcg aagcttaatg atgctgaacg
    12001 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    12061 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    12121 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    12181 ccagagtaag atacaagcta attttggttt gtctaaacaa gaggctgaag aattaacgaa
    12241 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    12301 attgattttg gtaaagcgta atttaggaga tttaaataat caagatttac aaaatatcac
    12361 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    12421 tgtaaatggc ttaatggtca atttcggctt gagtgctcaa gatgcaatgg atttaatggt
    12481 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    12541 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    12601 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    12661 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    12721 aaaagctttt gaagagtgga aaaatggcgg aggaacacaa aaagacgtta ttaattccat
    12781 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttcgacaat
    12841 ttggtctgcg cttggcgaag ataatgcgat gaaagttgtc caatctttaa ctgatgttaa
    12901 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    12961 gttgcaagag ttgaacggga aaatagctga attaaaggat tcattagctc ctataggcaa
    13021 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    13081 taataatctt ccacaaccag tacaggattt cattgttgct ataggtggtc ttacagcagc
    13141 atttggcata ctgttaccgg caatactagc cgtgtcatat ttatttggtc cgatgatgct
    13201 tataattgga ggaattatag ctgttatagc aggcgttatt gtggcaatta agaactgggg
    13261 tgcaattact gactggttta gtggtttatg gaaaaaattt actgattggt tgggtgatac
    13321 ttgggaaagt ataaaagacg gagcctcatc agtttgggat ggggttaaag aaacctggtc
    13381 tggatttgta gattgggttc aagatatttg gcaaggagtt tctgattggt ttggagagct
    13441 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag aaacttggca
    13501 aacattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    13561 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    13621 agctggtatt aatattatcg tgggtatttt tgatgtagct ggacagttat tgggcgcagc
    13681 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    13741 acaatggata agtatcgctt ggaatgggat tgtaactaca ttccatacta tatttgatcc
    13801 agtagtggca tggtggaatg gtatatggac agctattagt actacggctt caaatatttg
    13861 gaattcaatt agtgcaacag cttctagtat ttggaacagt atcaagaata caatcactag
    13921 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    13981 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggaata gtgtgactag
    14041 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    14101 atcttcgtgg attagcggaa tttggaacgg tatcaaaaat actgctttga atctttggaa
    14161 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    14221 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gtacaattgc
    14281 taacatatgg gaaggcatca aaaagactgt tagcgctcca attgattgga ttagagacaa
    14341 aatcagtggc atttttgata atttgaatat ttctatacca catattccgt taccggaatt
    14401 tatcatggaa ggcagcttta acccgctaaa aggtcaaatc ccccatttgc gtgtaaaatg
    14461 gcatgctaaa ggaggtatct ttactaaacc aactttacta ggtggaatga acggtgtcgg
    14521 tgaagcagga cctgaagcag ttttaccttt gaaaagatct gttttgcaag aaattggtga
    14581 tcgtatcttg agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa
    14641 ctacgaattc aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac
    14701 tcaacaaatc attgatagca ttacaaaaat tcaaaatgat aatgcttcgg catggcgtta
    14761 aacaggagag tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat
    14821 tgtatacatt ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa
    14881 agaccaacca taccttctcc aaaaagacag agagttgaaa cttctgtttt aggtaggtta
    14941 ggtggcttag tacaagatta ctcgtttgaa gatatgtcgt ttacattgca ctataactat
    15001 ttagaggatg tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg
    15061 ttaaactatg caaagaaatt agaattctct gacgatccca acgtctatta cgttatccag
    15121 actatcgata ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat
    15181 attactgcga aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca
    15241 cagtcattta acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact
    15301 ccttcagcta cttcatgcca gttcatctta aatgattatg tgtttagttt cgaaggctta
    15361 gtagcaggaa ctgatgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg
    15421 gacggagata ttttagatcg gtccaacaaa atgaagacca tgcaatatcc gacattgcaa
    15481 gtggatatca atcattttaa ttgcactggt ttgagcaaaa tacaaattta tcgtaacggg
    15541 ttaaggtagg tgaaatagat gatcgataat ttaataacta tttacgataa aaatgacgcg
    15601 aataatttag ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta
    15661 acagctactg ttagcaataa actaaacgga gccgagatat ttcagggtac ttattcaata
    15721 aacggaacta atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa
    15781 aatcgagcaa aacagcgtct acggatttat tatgcaaaaa cttctgtaat aggcaatacg
    15841 atagaagtaa aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat
    15901 gacagcggaa cagaaaagat cactgctact caggcatggc aaaacgcaaa agttttagcg
    15961 aaaccagcta tcccttcgca gttttctttc tcgtcattag tagatacgct tgctaatgtg
    16021 aagatagaaa aggcgaattt tttagaattc tttggtggaa aagagggatc tattctagat
    16081 cgatttcatg gtgagtttct aaaagataat aacacattac gtcatgaaaa aagtctaggt
    16141 acggatcata aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagatt
    16201 gatgctcaaa gtgttttagt tggagtttat ccattcatta gcagctcttc agaaggagaa
    16261 gacgagatca ctctaccaga agaagttatt ttcacggatt acgtggatga ttatcctgct
    16321 ggatatgttt cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa
    16381 gctgctaaag actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg
    16441 attgaattag taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa
    16501 gtttcgatgg gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg
    16561 agaattgtgg aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga
    16621 aacgtcaaaa caaacttctt agaaaacaca gagaataatg tcagcaattt gattaatgat
    16681 gccattgatc aattgaaaaa cggtggcgaa atcagtgatt tactcaatga tattgtagat
    16741 catcaaactg atatgattac tggtcaaaat ggtggttatg ttttattaga tcctaaagaa
    16801 gcgcctagtc gtattttgat tatggacaca ccagataaga ataccgcaag gaatgtttta
    16861 caaatcaaca atgctggtat tggtttttct aaaactggca ttaatggaac atatgaaacg
    16921 gcatggacgt tagatggcgg attcaatgcc tcgtttatta cggctggtga aatagtagga
    16981 attactatta gaggtactac attaattagt gatggtgctg attatagaac aagtattgct
    17041 aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca
    17101 cgtgattatg taagtgctga tgccggtatt gtatcataca ccatgaaaac tggtggtggt
    17161 ttcatgatta gaaatccaca gggtaacttg gtttttagta cgtgggataa tggtaataac
    17221 agaccgtttc tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatgtaact
    17281 tctggcgacg gtagttcttt aagcattaat ggtagtgcgg gtaactcatg ggaatttaag
    17341 gtagctggta ggactatgaa atttactagt gatggtatgc taacgttacc aggttgtttt
    17401 tttggttcat gggaagatgg gaaacttgct aggtttgaac aatcaacggt acaagtatat
    17461 aaagatttta ctgttagagg tactaaaaac tcaactgtac caacagaaca ttatggacaa
    17521 cgactattga acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt
    17581 acaggtgaca atggtaaagt tcgtgttgat attgacccca tgtttgctga aacagtaaat
    17641 ctaagtcggt atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat
    17701 gaagatattg accatttcat cattgaaact agtaagccaa acgtattagt tagatggaat
    17761 ttagtggcac accgtctagg gtatgaagat attagattaa aagaggatac agcatatgat
    17821 agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta
    17881 gcagtttata taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta
    17941 tggctcgtca aggtgataaa gggattacgg tgacggttaa accgtttcta aatggcttgc
    18001 agatggatac gagtggcgga acatttactt taaaaggaac aacaccatct aaccgttacg
    18061 tagataatgt tgcaactagt gtaactagtg aagaagtcac gttttctctt gatggcacat
    18121 ttatgagtga agcaggatat tataaacact gctacgtaga atatagaaaa gacaatcaaa
    18181 ttttaacaac gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc
    18241 aagccgatga atatgtttcg caattagaag agttgattcg aaagtacaac gaaacttttg
    18301 atgcttttat ggctgaaatc aaaggtagag tggatagctt aaatcaacag attactgatt
    18361 taactggtca agctaaaacg ctacaagaca agttagatgc tctgaaagaa gaaatttcta
    18421 agttaggtaa cttacaagtg atgtacagta acagcatcga cttcggggac tatgattatt
    18481 cggagaaccc gaatttaatg ccatatatta cagaaccttg ggttggacct ttgttaggta
    18541 atggacatac agttaaagat agtgttaaaa gggttataac tcatactaaa actagaacag
    18601 ctaattccgg cgacatatta tcgcttggct taggtattcc gtgtacagcg gaagctaata
    18661 ataggtatct tataaccact ttacgaccat ctacgacata cacattaagc gttactatga
    18721 gtgtcggttc agattggact ggagagacca atactatagg ggttaggctt agatatttaa
    18781 atgaacaagg tggtatagaa ctgcccatta atgcattaat accagcaaat gtagaacgcg
    18841 ataaaatggt cactcataca tttactggta taacaaaaga taatgtaact agcataacaa
    18901 actgttatgt tgaaatattc tcacttaata gtgaatataa aggaacggtt agtgtcagct
    18961 atgacgttaa gttaaaagca cactacccta acttactaga tggcccttac tggttaggta
    19021 aagttccttt aggcgagaat attgctgacc ctacagttgt ctttccacac aaaactagtg
    19081 aatatatggt ttatggtaga cgcaatacag aaaactatat cgctgaccaa acatatacca
    19141 tatcaatgaa agcaactaaa ctaacagttc aatcgtttgc cgtgtatatt gctgctggcc
    19201 gcgttaaagt aggagacatg aagcctactg aggggttagc taatacatgg gagttaactt
    19261 ttactgtaac taagcaacat atagatagtg gggtaactaa ttatctggaa atataccaat
    19321 acccgtcagc tacaaaagga gctgttcaaa ttgagtggct caaactagaa aaaggcaata
    19381 ctcgaacccc gaatattagt gagtataaat atcgtggtac tggtatgcgt gattcaaaca
    19441 atccaaaaga ttatgtttgg gatctagcac cagaatatgt cgaagataat ttggccacag
    19501 atattaaaat ttctgaaatt actggtaaag caaacaatta taccgatggg aaagtatcgg
    19561 agattaattc gcagttgact gcttcaatta atgaagtaga caccacagct aaggatgctc
    19621 aaacaaaagc gaatgctaat gcgactgcta tagatgaatt agacaataag atcgatgaac
    19681 gcattaatga tacagctact accacattaa cagttacaaa cgggaatacc ggatcagcaa
    19741 agctttatcg tgaaggaaaa acagtttcta tatattttgt ggctttaaac ggaaaaagca
    19801 gtggtggaaa tgattcaacg atactaacaa ttccagaagg ctatcggcca ccaattagtt
    19861 ttgagcaact ggttggctcg atagaccgtt ctactttgaa cagtgctcag ttatctattg
    19921 gtgcagatgg agccattaaa tggcgaagaa actcaagtta tggatcggat tatacctttg
    19981 caattactta cacgatttag aaaagcgtga atcgatatga aggcagcata tagaccaatt
    20041 gaaccttacg gattcgagca aatcattgtg aatgatgaag aacatttacc ggaagaatgc
    20101 acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg ggagggaaat
    20161 aaatgaaaaa catttggaaa tatggacgta ctggcggaga gtacgcagga aaagtattgg
    20221 acgacatgct tgtatccgtt ccttacacag atcagccacc gcttgaagga attcgtgctg
    20281 atggcgaacc gttaacaatc gctgatcaaa tgtttgatcc taaattgaat caatggatta
    20341 ttttagcgaa cgcactagat cacaacgatt taaacaatct caaagcaatg tatgagtcgt
    20401 tagaaaatga gaacggcgat ttaaaacaga tcaatgccaa actcatgcta agcgatgtag
    20461 caattaaaca ggaaaatact gcattgaaag aaaaagcgga tagtttagca caaatcaatt
    20521 caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt aaagagcaac
    20581 taaatccagc ttcaaaggga ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg
    20641 tatgattggg gctgtttcac taacgaacaa gtaatggttt tcgttccgtt gtgcattact
    20701 gaagaaaaag cagataaaat cattagcaa
//
LOCUS       DACEJR010000051        20285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEJR010000051 DACEJR010000000
VERSION     DACEJR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.051"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            140..460
                     /locus_tag="IW651_001955"
     CDS             140..460
                     /locus_tag="IW651_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814676.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IW651_001956"
     CDS             912..1112
                     /locus_tag="IW651_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0814677.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IW651_001957"
     CDS             1259..4048
                     /locus_tag="IW651_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0814678.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IW651_001958"
     CDS             4095..4622
                     /locus_tag="IW651_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0814679.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IW651_001959"
     CDS             4643..5833
                     /locus_tag="IW651_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0814680.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IW651_001960"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IW651_001960"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0814681.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IW651_001961"
     CDS             8224..8898
                     /locus_tag="IW651_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0814682.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IW651_001962"
     CDS             8895..9236
                     /locus_tag="IW651_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814683.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IW651_001963"
     CDS             9266..9625
                     /locus_tag="IW651_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0814684.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IW651_001964"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IW651_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IW651_001965"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IW651_001965"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0814685.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IW651_001966"
     CDS             12541..14007
                     /locus_tag="IW651_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0814686.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14622..15161
                     /locus_tag="IW651_001967"
     CDS             14622..15161
                     /locus_tag="IW651_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0814687.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15235..15675
                     /locus_tag="IW651_001968"
     CDS             15235..15675
                     /locus_tag="IW651_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0814688.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15688..15897
                     /gene="copZ"
                     /locus_tag="IW651_001969"
     CDS             15688..15897
                     /gene="copZ"
                     /locus_tag="IW651_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0814689.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15897..18083
                     /locus_tag="IW651_001970"
     CDS             15897..18083
                     /locus_tag="IW651_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0814690.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18103..20274
                     /gene="copB"
                     /locus_tag="IW651_001971"
     CDS             18103..20274
                     /gene="copB"
                     /locus_tag="IW651_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0814691.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6776 a         3527 c         4407 g         5575 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aagtttcggc ttgtttttcg cagaagctac ttctggaaca ccgtgaatca gaggttgtga
    14161 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagcccc tcatattttt
    14221 cgttttgctg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggta
    14281 gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacaa cttctcatgt
    14341 ttgttgacta ttgtgccttg tgaccgaggt gttggcagga gaacaccgtt taaggaagag
    14401 gttgtaaaat cagcgatttg acctgagtat tagaggaaca aacgaaaaaa tagctcctca
    14461 tattttgccg ttttttgagt taatgctgaa ggtcagctga ttgaacacca tttattcgaa
    14521 acaagggact ttgacacgac ttttgtcaca gtcccttgtt tttttgcggc gttataagtg
    14581 gtatgatcga accaaattcc aaagagaagt gagggagaag aatgaagatc atcgtgattg
    14641 gacaaagtgg gtcgggaaaa tcgaccctcg caaggaagat caaagaaatc accgactttc
    14701 cgctactgcc gctagacctt ttatggcata caacagacta cagtattcag gcaaaaaagt
    14761 ggtttttgca agaacagcaa tcgtttatgg tatctaatcc gtcatggatt gttgaaggaa
    14821 attatacttc tacattagag gaaaggatta aagaagcaga taagatcatt tggcttaaag
    14881 tgccgcgtta tctcgccatc taccgagtag tttggcgttc tctaaaaaga aagataaaca
    14941 aaaaatctcg gccagatatg cctgaatcat tttcagagaa actaaacagg gaatacttag
    15001 aatttttatc ttttatctgg cattttgaag aaaacaatga accgaaaata cagcagctga
    15061 tccaagtagc taatgcaaga aataaactga tcatccttaa aacaagacga gagaaacaag
    15121 aatttttaac taaattgaaa atagagagca aaaataattg acagtttttg tttacaagcg
    15181 taatctgtat tcataagcta atgattacat ttgtaaacga aaggtgtgaa ataaatggaa
    15241 aaagtcaaaa cgccagtaaa gatcagtgat tcagagtggg agatcatgcg tgttatttgg
    15301 acacttggac agacgacagc ccaagaaatc acgcagatct tagctgattc gatggattgg
    15361 aaaccagcaa cgatcaaaac tttgctggga cgtttggtaa aaaaagaagt gatctggaca
    15421 gaacaagaag gaaaaaaatt catttatcat ccagctgtct cggagatgga aacagttcgc
    15481 agtgcgacgg aaaatctttt ttcccatatc tgtgcaaaaa ggattggaga gacgattgcg
    15541 gatctagtag aagaagccac tttgactcaa gaagatatca atgagatttt gaaacaactg
    15601 gaacaaaagc aaccagtaga tacgatcgaa tgtaactgta tcccaggtca atgtgaatgt
    15661 aaacaacatc aataggagga agaaaaaatg aaacaacaat tttcaatcga aggtatgagc
    15721 tgcaatcatt gtgcagcaag agtagaagaa acagtaagcg cacttgacgg cgtgcaaaaa
    15781 gtgaaagtaa atttgaaaaa agcaaacggt acggtcaagt ttgatgaaac aaaagttcaa
    15841 tccgaaaaga tttgtcaagc aatcaatgga ttgggttata aagcagaggt gatttgatgg
    15901 caacagcaga aaccaaaatg gatacgttcg tcattacagg tatgacttgt gcaaattgtt
    15961 ctgctaggat cgaaaaagag ctgaacggac aacctggtgt ggtcaatgcc acagtcaact
    16021 tggccactga aaaagcaagt gtcaaatatg aagggacgac cactgaaaaa ttaatccaaa
    16081 gcgtagaaaa catcggttat ggtgcaattt tgtacgatga agcccacaaa caaaaaattg
    16141 cagaagaaaa gcaagcgtat ttgaaaaaaa tgctattcga tcttatattg agtacggttt
    16201 tgactttgcc gttgatgttg tcaatgattg ctatgatgtt aggaagccat gcggcgattg
    16261 ttcacttttt tcattttcca atcgtccagt tagtgctgtc tgctcctgtc caattttatg
    16321 tcggtgctcg tttttataaa ggtgcgtacc atgcaatcaa gacgaaagca ccaaatatgg
    16381 atgtattggt agcaattgga acatcggctg cctttgcatt gagcatttac aacggatttt
    16441 tccgtggaca cccccaagat ctttattttg aaagcagcag tatgatcatc acgctgattt
    16501 tgctcggcaa atatttggaa catacagcaa aaacgaaaac aggtaatgca atcaaacaat
    16561 tgatgtctct tcaaacaaaa accgcccaag tgataagaaa cgggaaagaa gaaacgttag
    16621 cgattgaaga agtagtcgtt ggtgatcaac tagtcatccg tcctggtgag cagattccag
    16681 cagatggtcg aattatttca ggaagcagtg cgatagatga aagtatgctt actggtgaaa
    16741 atcttcctgt agaaaagaat ccggatgaca cgctttttgg cgggacaatc aatacgaatg
    16801 gtctgctaca catggaagtc acacaagtcg gcaagcagac agtattagcg caaatcattc
    16861 aaatggtgga agatgctcaa gggagcaaag ccccaatcca aaaaatcgca gatcggatct
    16921 cagggatttt tgttccgatc gtattagtca tcgcgtttat cacgctcata gctacaggtt
    16981 tgatcactgg tgattggcaa ctcgcattga tccatagtgt ctctgtatta gtcattgcgt
    17041 gtccatgtgc attaggttta gcgaccccaa cagctattat ggtaggaacg ggtgttggtg
    17101 cacgaaatgg aattttaatt aaaggcggag aagcattaga agcagctgct cacttagaca
    17161 gcattgtttt ggataaaact ggaacgatta cagaaggaaa gcccaaagtt acagatctag
    17221 tcggttcaaa agaagtccta tctatttttt atactttaga acaagcttct gaacatccac
    17281 taggtaaagc aatcgtggaa tacggaaaac tgcaagaagc agcaacatac gatatgattg
    17341 attttacagc ccacccaggt gctgggatca gcggtacgat taatggagta cgctattttg
    17401 cgggtacacg taaacgatta attgaattga acctatcatt tgatgaatac caagaacacg
    17461 cgttagaatt agaacagcag ggaaaaacag tcatgttttt agctgatgaa aagcaagtga
    17521 tcgggtggat tgccgtggct gatcaaatca aactagaagc caaacaagca atcaagcagc
    17581 tccagaataa aggattagat gtttttatgc tgacaggaga caacaaactg gcagccgaaa
    17641 cgattggtaa acaagttggt attgatccaa agcatatctt tgctgaagta cttccagagg
    17701 ataaagcagc ctacgtagaa aaacttcaga aagatggcaa aaaagtcggt atggcaggcg
    17761 atggaatcaa tgatgcaccg gccttagcat tagctgatgt cggtatggca atgggaagtg
    17821 gaacggacat tgcgatggaa acagccgatg tgacgctgat gaatagcagt ttggcatcca
    17881 ttgcacaaac aatcgagttg tctaggttga cattgagaaa aatcaaacaa aatcttttct
    17941 gggcttttgt ctataatacg attggtattc cttttgccgc actaggcttt ttgaatccaa
    18001 tcattgcagg aggagcgatg gcgtttagtt ccgtcagtgt attgttgaac tccctgagct
    18061 taaatcgaca catgaaaaag taagagaagg gttggagtaa aaatgaatgg gaacgatatg
    18121 gagaaaaaac atgagcataa aaaagaagta gtaaacaaag aaagtacaga atctcatgaa
    18181 aaacagatgg agatgaaaca tgaccatagt cagatggatc attccatgca catgggacat
    18241 aaccatggag atatagcaaa cagcaaacaa atggaccatt cgatgcacat ggatcacgaa
    18301 cacggcggca tggatcattc catgcacatg gggaatttca aacaaaaatt ttggctatcg
    18361 cttatcctag ctatcccaat cattcttttt tcaccaatga tgggaatgga atttccattt
    18421 caagtaacgt ttcctggttc tgattggctc gtattgatct tagcaactat actatttatc
    18481 tatggcggtc agccattttt aagtggagct aagatggaat tgaaacaaaa aagtcctgct
    18541 atgatgacac tgatcgcgat ggggattaca gttgcatata tttatagtgt ctattcgttt
    18601 atcgcaaatc tgctcaatcc acatacacac gtcatggatt tcttctggga attggctact
    18661 ttgatcgtta ttatgctact aggtcactgg attgaaatga atgctgtgtc gaatgccagc
    18721 aatgctttac aaaaactagc agaattactg ccagaatcag tgaaacgctt gacgaaagat
    18781 ggaaaagaag agacagtttc attgaaagaa gtaaacgaag gtgatcgttt gatcgtccgg
    18841 tctggtgata aaatgccaac agatggtgta atcttgaaag gtgaaacaat tgttgacgaa
    18901 tcagctgtga ctggcgaatc aagaggaatc aaaaaacaag ccaatgacaa agtgattggc
    18961 ggttctatca atggggatgg aacgatcgaa atcgaagtaa ctggaactgg tgaaaatggt
    19021 tatctagcga aggttatgga aatggtgcga aaagcacaag gagaaaaatc caagctagag
    19081 tcattatctg acaaagtggc aaaatggctg ttttatgtcg cattaattgt gggtatcctg
    19141 gcattcatcg cctggttgtt cttgacagat cttcctaatg cattagaacg tatggtaaca
    19201 gtctttatca tcgcctgtcc tcatgctcta ggtttagcta tcccattagt tgttgctcgc
    19261 tccacatcaa tagcagctaa aaacggatta cttttgaaaa accgcaatgc actggagcaa
    19321 gccaatgatc tagatgtgat catgctggac aaaacaggaa cactaacaga aggaaaattt
    19381 actgtaacgg gtgtcgaagt acttgatgat gcgttcaata aaaatgaaat ccttcaatat
    19441 ctaggagcct tggaagcgaa tgccaatcat ccattggctg ttggcattat gaattatttg
    19501 aaagaacatg aaatcaagcc ttatcaagct gaaaatctaa aaaatctttc aggagtcggg
    19561 ctagaggcaa cagtcaaaaa acaacaagtg aaaatagtta atgaaaaaga agttgagcgt
    19621 ctcggattaa acgtagaaca agcattattg aaaccttacc aagaacaagg aaataccatc
    19681 agcttcctga ttttagaaaa tcatctagct gcaatcgttg ctttaggtga cgtagtaaaa
    19741 acagaagcaa aagaatttat ccgaacattg aaagaaagaa aaatcactcc tgtcatgctt
    19801 actggtgaca ataaaaacgc tgcccaagca gtagcagatt atttagggat cgaagaatat
    19861 tatggcggtt tattaccaga tgataaagaa gctgttgtcc agaaatacct tgaccaaggg
    19921 aaaaaagtaa tcatggtggg tgatgggatc aatgatgcgc caagtttagc ccgtgcttca
    19981 atcgggatgg caatcggtgc agggacagat atcgcaatcg attctgcgga tgtcgtattg
    20041 acaaacagcg atccaaaaga tattttgcat tttcttgatc tagccaagca aacgcgaaag
    20101 aaaatgatcc agaacctctg gtggggggca ggttacaata ttcttgcaat tccgctagca
    20161 gccggaattc ttgcgccaat tggtatcata ttaagtccag cagttggtgc tgtcttgatg
    20221 tcgttaagta cggtagttgt agctctaaat gctttgacat tgaaaatcaa ataataatga
    20281 aaaaa
//
LOCUS       DACEJR010000052        18895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEJR010000052 DACEJR010000000
VERSION     DACEJR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.052"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..251)
                     /locus_tag="IW651_001972"
     CDS             complement(21..251)
                     /locus_tag="IW651_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814692.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IW651_001973"
     CDS             complement(687..1253)
                     /locus_tag="IW651_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0814693.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IW651_001974"
     CDS             complement(1253..2209)
                     /locus_tag="IW651_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0814694.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IW651_001975"
     CDS             complement(2209..2865)
                     /locus_tag="IW651_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0814695.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IW651_001976"
     CDS             3235..5253
                     /locus_tag="IW651_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0814696.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IW651_001977"
     CDS             5680..8094
                     /locus_tag="IW651_001977"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0814697.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IW651_001978"
     CDS             8442..9995
                     /locus_tag="IW651_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0814698.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IW651_001979"
     CDS             complement(10376..12160)
                     /locus_tag="IW651_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814699.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IW651_001980"
     CDS             complement(12175..13896)
                     /locus_tag="IW651_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814700.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IW651_001981"
     CDS             13996..14757
                     /locus_tag="IW651_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0814701.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IW651_001982"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IW651_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="IW651_001983"
     CDS             16664..>18895
                     /locus_tag="IW651_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814702.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DACEJR010000053        17202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEJR010000053 DACEJR010000000
VERSION     DACEJR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.053"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..328)
                     /locus_tag="IW651_001984"
     CDS             complement(26..328)
                     /locus_tag="IW651_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0814703.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IW651_001985"
     CDS             complement(353..2446)
                     /locus_tag="IW651_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0814704.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IW651_001986"
     CDS             complement(2935..3699)
                     /locus_tag="IW651_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0814705.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW651_001987"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW651_001987"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0814706.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IW651_001988"
     CDS             complement(4704..5780)
                     /locus_tag="IW651_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0814707.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IW651_001989"
     CDS             complement(5804..6397)
                     /locus_tag="IW651_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0814708.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW651_001990"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW651_001990"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0814709.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IW651_001991"
     CDS             complement(8067..8366)
                     /locus_tag="IW651_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0814710.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW651_001992"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW651_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0814711.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IW651_001993"
     CDS             complement(10121..11032)
                     /locus_tag="IW651_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0814712.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IW651_001994"
     CDS             11152..12009
                     /locus_tag="IW651_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0814713.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IW651_001995"
     CDS             complement(12196..12558)
                     /locus_tag="IW651_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814714.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IW651_001996"
     CDS             12904..14343
                     /locus_tag="IW651_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0814715.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IW651_001997"
     CDS             complement(14350..16902)
                     /locus_tag="IW651_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0814716.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..>17202)
                     /locus_tag="IW651_001998"
     CDS             complement(17016..>17202)
                     /locus_tag="IW651_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0814717.1"
                     /translation="IKEKKRNTTQRYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIW
                     FVFLNFVYKRISNRNIS"
BASE COUNT         4872 a         3650 c         2968 g         5712 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa ta
//
LOCUS       DACEJR010000054        17005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEJR010000054 DACEJR010000000
VERSION     DACEJR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.054"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..124)
                     /locus_tag="IW651_001999"
     CDS             complement(<1..124)
                     /locus_tag="IW651_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0814718.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IW651_002000"
     CDS             complement(228..539)
                     /locus_tag="IW651_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0814719.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IW651_002001"
     CDS             complement(541..738)
                     /locus_tag="IW651_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0814720.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IW651_002002"
     CDS             complement(1132..2859)
                     /locus_tag="IW651_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0814721.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IW651_002003"
     CDS             complement(2852..4045)
                     /locus_tag="IW651_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0814722.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="IW651_002004"
     CDS             complement(4232..4876)
                     /locus_tag="IW651_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0814723.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="IW651_002005"
     CDS             complement(4970..5104)
                     /locus_tag="IW651_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0814724.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IW651_002006"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IW651_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0814725.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="IW651_002007"
     CDS             complement(7235..7708)
                     /locus_tag="IW651_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0814726.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="IW651_002008"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="IW651_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0814727.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="IW651_002009"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="IW651_002009"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0814728.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="IW651_002010"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="IW651_002010"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0814729.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="IW651_002011"
     CDS             complement(11755..12894)
                     /locus_tag="IW651_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0814730.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="IW651_002012"
     CDS             complement(13203..13916)
                     /locus_tag="IW651_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0814731.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IW651_002013"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IW651_002013"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0814732.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IW651_002014"
     CDS             15105..16637
                     /locus_tag="IW651_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0814733.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="IW651_002015"
     CDS             complement(16828..>17005)
                     /locus_tag="IW651_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0814734.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACEJR010000055        16596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEJR010000055 DACEJR010000000
VERSION     DACEJR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.055"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW651_002016"
     CDS             complement(45..779)
                     /locus_tag="IW651_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0814735.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IW651_002017"
     CDS             1105..1515
                     /locus_tag="IW651_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0814736.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IW651_002018"
     CDS             1508..2209
                     /locus_tag="IW651_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0814737.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IW651_002019"
     CDS             2209..3822
                     /locus_tag="IW651_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0814738.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IW651_002020"
     CDS             3812..4033
                     /locus_tag="IW651_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0814739.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IW651_002021"
     CDS             4030..4791
                     /locus_tag="IW651_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0814740.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IW651_002022"
     CDS             5172..5681
                     /locus_tag="IW651_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0814741.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IW651_002023"
     CDS             complement(5751..6290)
                     /locus_tag="IW651_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0814742.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW651_002024"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW651_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0814743.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IW651_002025"
     CDS             7357..8154
                     /locus_tag="IW651_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0814744.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IW651_002026"
     CDS             8182..8817
                     /locus_tag="IW651_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0814745.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IW651_002027"
     CDS             8837..9610
                     /locus_tag="IW651_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0814746.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IW651_002028"
     CDS             10018..10815
                     /locus_tag="IW651_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0814747.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IW651_002029"
     CDS             10793..11206
                     /locus_tag="IW651_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814748.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IW651_002030"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IW651_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0814749.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IW651_002031"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IW651_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0814750.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IW651_002032"
     CDS             15983..16543
                     /locus_tag="IW651_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0814751.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2964 c         3599 g         4646 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc cgtttt
//
LOCUS       DACEJR010000056        16529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEJR010000056 DACEJR010000000
VERSION     DACEJR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.056"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..417)
                     /locus_tag="IW651_002033"
     CDS             complement(142..417)
                     /locus_tag="IW651_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0814752.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IW651_002034"
     CDS             complement(660..1970)
                     /locus_tag="IW651_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0814753.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW651_002035"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW651_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0814754.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IW651_002036"
     CDS             complement(3516..4196)
                     /locus_tag="IW651_002036"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0814755.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IW651_002037"
     CDS             complement(4245..4880)
                     /locus_tag="IW651_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0814756.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IW651_002038"
     CDS             complement(4946..6349)
                     /locus_tag="IW651_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0814757.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IW651_002039"
     CDS             complement(6336..7367)
                     /locus_tag="IW651_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814758.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IW651_002040"
     CDS             7398..7619
                     /locus_tag="IW651_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0814759.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IW651_002041"
     CDS             complement(7784..8521)
                     /locus_tag="IW651_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0814760.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IW651_002042"
     CDS             complement(8674..10014)
                     /locus_tag="IW651_002042"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0814761.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IW651_002043"
     CDS             complement(10251..11774)
                     /locus_tag="IW651_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0814762.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IW651_002044"
     CDS             complement(12152..12763)
                     /locus_tag="IW651_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0814763.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IW651_002045"
     CDS             complement(13093..13809)
                     /locus_tag="IW651_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0814764.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW651_002046"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW651_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0814765.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IW651_002047"
     CDS             complement(14368..15165)
                     /locus_tag="IW651_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0814766.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IW651_002048"
     CDS             complement(15135..15506)
                     /locus_tag="IW651_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0814767.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW651_002049"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW651_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0814768.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5509 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttcttttt
//
LOCUS       DACEJR010000057        16356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEJR010000057 DACEJR010000000
VERSION     DACEJR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.057"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..210
                     /locus_tag="IW651_002050"
     CDS             <1..210
                     /locus_tag="IW651_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814769.1"
                     /translation="NGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYI
                     PNETITKTDMALMNREAEKIINKLT"
     gene            complement(246..548)
                     /locus_tag="IW651_002051"
     CDS             complement(246..548)
                     /locus_tag="IW651_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814770.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(797..997)
                     /locus_tag="IW651_002052"
     CDS             complement(797..997)
                     /locus_tag="IW651_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0814771.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1302..2534)
                     /locus_tag="IW651_002053"
     CDS             complement(1302..2534)
                     /locus_tag="IW651_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0814772.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2791..3360
                     /locus_tag="IW651_002054"
     CDS             2791..3360
                     /locus_tag="IW651_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0814773.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3538..3978)
                     /locus_tag="IW651_002055"
     CDS             complement(3538..3978)
                     /locus_tag="IW651_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0814774.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4136..4900
                     /gene="map"
                     /locus_tag="IW651_002056"
     CDS             4136..4900
                     /gene="map"
                     /locus_tag="IW651_002056"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0814775.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4932..5855
                     /locus_tag="IW651_002057"
     CDS             4932..5855
                     /locus_tag="IW651_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0814776.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5931..7070
                     /locus_tag="IW651_002058"
     CDS             5931..7070
                     /locus_tag="IW651_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0814777.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7063..7863
                     /locus_tag="IW651_002059"
     CDS             7063..7863
                     /locus_tag="IW651_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0814778.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7863..8690
                     /locus_tag="IW651_002060"
     CDS             7863..8690
                     /locus_tag="IW651_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0814779.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8668..9402
                     /locus_tag="IW651_002061"
     CDS             8668..9402
                     /locus_tag="IW651_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0814780.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9502..10368
                     /gene="rfbA"
                     /locus_tag="IW651_002062"
     CDS             9502..10368
                     /gene="rfbA"
                     /locus_tag="IW651_002062"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0814781.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10382..10954
                     /gene="rfbC"
                     /locus_tag="IW651_002063"
     CDS             10382..10954
                     /gene="rfbC"
                     /locus_tag="IW651_002063"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0814782.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10976..12004
                     /gene="rfbB"
                     /locus_tag="IW651_002064"
     CDS             10976..12004
                     /gene="rfbB"
                     /locus_tag="IW651_002064"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0814783.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12102..12953
                     /gene="rfbD"
                     /locus_tag="IW651_002065"
     CDS             12102..12953
                     /gene="rfbD"
                     /locus_tag="IW651_002065"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0814784.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12987..15020
                     /locus_tag="IW651_002066"
     CDS             12987..15020
                     /locus_tag="IW651_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814785.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15064..16344
                     /locus_tag="IW651_002067"
     CDS             15064..16344
                     /locus_tag="IW651_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0814786.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         5052 a         2812 c         3375 g         5117 t
ORIGIN      
        1 aatggaagta gcggaaaagg aagccaccaa accgtttgga atttgttttc aacagttaag
       61 gaattgaata ccttaggaat acataatagt gggtatagaa tgttagagaa aagaaaatgt
      121 gctgactata ttcctaatga aaccattacg aaaacagata tggcgctgat gaatcgagaa
      181 gcagaaaaaa taataaacaa actcacctaa tgaatttcaa gcgagtttgt ttgttatttt
      241 tttatttaat ctttgtatct ttctggatca acgaaagtat actttatata gtcataacgc
      301 cgatgatcgc tacgtgcgtc cggcacgtca gtcacgatat caaacaaaaa gtatacaccc
      361 ttcttcattc tagttttcgc agcaggaatt ttgaaatagt tcttattaga atagtagaga
      421 ttgattaata agctatcttc gattgctaaa aagaaaactt ctgaattcca tactttataa
      481 aaatctttga taaatctatt cgaagggtca aatttaaacc ataattgtgt ttttccttcc
      541 atcagcataa aagttcacct caaaaagagt atacgaacga atgttctttt tgtgaaggcg
      601 gaatttaata tactgaataa tagaaaacaa gtacttgtgc caaattatgt gccaaaaaaa
      661 ttcgaattca acaaaatgta aacaaataga aaaccttcta tattaagatt tcctgcaata
      721 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
      781 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
      841 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
      901 tatgtcttag tccctcaata aacagtcgaa gagaaaatag gcaaaaattg attactaaag
      961 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaacagatt cgaattaaac
     1021 gatttcgatt ggcttatacc agtcctatcc gacgaaagat atatcgttct taactctagc
     1081 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1141 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1201 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
     1261 ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt
     1321 cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat
     1381 cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca
     1441 tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga
     1501 ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt
     1561 gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc
     1621 agttttgcta agacatcttc tccttgttcg gcagaaaaat caatgacttt tccatcttta
     1681 aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa
     1741 atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt
     1801 gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta
     1861 ggcaatccga taatgatatc tgtaccggga gcagtatagt gcagggcaga aaattgttct
     1921 cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca
     1981 gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt
     2041 ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg
     2101 acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca
     2161 gccgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg
     2221 gcagaaacaa cactgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt
     2281 ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg
     2341 atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt
     2401 gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct
     2461 ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta
     2521 aaatcagata acataataaa aattcctcct caccattttt tctggtaact attagcatga
     2581 taccatttaa ttatgattca aacaatgagg cctttaaata aaaaaaagag gttatttttt
     2641 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
     2701 aaactaattt tgcatatcaa aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg
     2761 ttataaaaaa attaaggaga ttaaaaaaat atggcaagaa aaaagacgat tactagagaa
     2821 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     2881 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     2941 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     3001 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     3061 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     3121 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     3181 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     3241 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     3301 atgatacaag acagtataga tgctatcttg aaattcaaag aaccaataga aatcgattga
     3361 atatactcaa aagttccaca aatcagtaga taacaagaga tgtgtcaaaa taaggaaaag
     3421 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     3481 gaataaaaga gccgtacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     3541 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     3601 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     3661 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     3721 accgtaaatt tttcccgata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     3781 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     3841 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     3901 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     3961 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     4021 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     4081 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     4141 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     4201 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     4261 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     4321 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     4381 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     4441 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     4501 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     4561 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     4621 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     4681 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     4741 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     4801 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     4861 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     4921 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     4981 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     5041 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     5101 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     5161 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     5221 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     5281 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     5341 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     5401 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     5461 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     5521 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     5581 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     5641 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     5701 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     5761 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     5821 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     5881 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     5941 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     6001 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     6061 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     6121 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     6181 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     6241 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     6301 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     6361 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     6421 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     6481 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     6541 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     6601 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     6661 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     6721 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     6781 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     6841 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     6901 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     6961 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     7021 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     7081 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     7141 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     7201 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     7261 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     7321 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     7381 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     7441 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     7501 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     7561 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     7621 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     7681 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     7741 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     7801 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     7861 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     7921 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     7981 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     8041 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     8101 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     8161 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     8221 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     8281 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     8341 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     8401 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     8461 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     8521 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     8581 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     8641 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     8701 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     8761 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     8821 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     8881 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     8941 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     9001 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     9061 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     9121 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     9181 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     9241 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     9301 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     9361 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     9421 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     9481 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     9541 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     9601 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     9661 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     9721 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     9781 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     9841 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     9901 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     9961 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
    10021 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
    10081 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
    10141 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
    10201 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
    10261 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
    10321 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
    10381 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
    10441 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
    10501 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
    10561 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
    10621 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
    10681 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
    10741 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
    10801 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
    10861 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
    10921 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
    10981 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
    11041 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
    11101 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
    11161 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    11221 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    11281 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    11341 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    11401 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    11461 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    11521 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    11581 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    11641 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    11701 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    11761 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    11821 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    11881 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    11941 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    12001 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    12061 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    12121 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    12181 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    12241 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    12301 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    12361 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    12421 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    12481 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    12541 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    12601 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    12661 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    12721 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    12781 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    12841 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    12901 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    12961 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    13021 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    13081 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    13141 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    13201 tttttggaca gaatcgggag gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    13261 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    13321 acatgatcat tgcgtataat tctcctgcaa aggatattac ggttatcgga aaaccattca
    13381 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    13441 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    13501 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    13561 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    13621 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    13681 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    13741 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    13801 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    13861 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    13921 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    13981 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    14041 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    14101 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    14161 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    14221 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    14281 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    14341 atttctatgc gattacgcat tcgaaaatag ataattattt ttcggatttt gaaaccatcg
    14401 cagttgctgt gttgggaaca gtgattttag catttgcatt atttggctgg agacgcttgt
    14461 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    14521 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    14581 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    14641 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aaggaaatcg
    14701 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    14761 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    14821 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    14881 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    14941 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    15001 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg
    15061 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    15121 ttgcttttgt tgctgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    15181 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    15241 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    15301 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagattta
    15361 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    15421 gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta
    15481 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    15541 gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc
    15601 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    15661 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    15721 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    15781 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    15841 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    15901 ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca acgaggaaac
    15961 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    16021 tttgggattg ctactttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    16081 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    16141 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    16201 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    16261 tatgacgata tgacttatga tgatacatct tatggggaac aaccagatag cgctcattct
    16321 tccaggatcc aacgctatca ttgataggca caaaat
//
LOCUS       DACEJR010000058        16095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEJR010000058 DACEJR010000000
VERSION     DACEJR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.058"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(330..632)
                     /locus_tag="IW651_002068"
     CDS             complement(330..632)
                     /locus_tag="IW651_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814787.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="IW651_002069"
     tmRNA           complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="IW651_002069"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1389..2162)
                     /locus_tag="IW651_002070"
     CDS             complement(1389..2162)
                     /locus_tag="IW651_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0814788.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2306..2656
                     /locus_tag="IW651_002071"
     CDS             2306..2656
                     /locus_tag="IW651_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0814789.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2637..3353
                     /locus_tag="IW651_002072"
     CDS             2637..3353
                     /locus_tag="IW651_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0814790.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3353..4801
                     /locus_tag="IW651_002073"
     CDS             3353..4801
                     /locus_tag="IW651_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0814791.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4871..5380)
                     /locus_tag="IW651_002074"
     CDS             complement(4871..5380)
                     /locus_tag="IW651_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814792.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5370..6095)
                     /locus_tag="IW651_002075"
     CDS             complement(5370..6095)
                     /locus_tag="IW651_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814793.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6318..8438)
                     /locus_tag="IW651_002076"
     CDS             complement(6318..8438)
                     /locus_tag="IW651_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0814794.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8668..9846
                     /locus_tag="IW651_002077"
     CDS             8668..9846
                     /locus_tag="IW651_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0814795.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9862..10620
                     /gene="aroD"
                     /locus_tag="IW651_002078"
     CDS             9862..10620
                     /gene="aroD"
                     /locus_tag="IW651_002078"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0814796.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10643..11128
                     /gene="ybaK"
                     /locus_tag="IW651_002079"
     CDS             10643..11128
                     /gene="ybaK"
                     /locus_tag="IW651_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0814797.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11199..12452)
                     /locus_tag="IW651_002080"
     CDS             complement(11199..12452)
                     /locus_tag="IW651_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0814798.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TKTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12569..13918
                     /gene="gdhA"
                     /locus_tag="IW651_002081"
     CDS             12569..13918
                     /gene="gdhA"
                     /locus_tag="IW651_002081"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0814799.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14030..15376
                     /locus_tag="IW651_002082"
     CDS             14030..15376
                     /locus_tag="IW651_002082"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0814800.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15684..16070)
                     /locus_tag="IW651_002083"
     CDS             complement(15684..16070)
                     /locus_tag="IW651_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0814801.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5281 a         2867 c         3215 g         4732 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata taactgtaaa aaataatggg aaaaagactt ataattggag tggtagttaa
      181 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      241 acacaatctt aatactaact taaaaaatat cccttttata agtatggtga taaaatccgt
      301 tccgggctac cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
      361 gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg
      421 atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag
      481 tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa
      541 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      601 aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacgaa
      661 ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac atatatatta
      721 aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa cgaaaatctg
      781 cgaaaataaa aaatgctcaa acccttataa aataaggatt taagcatcta gcgaaatctg
      841 ctgaaatctc acaatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta
      901 aaaatctacg ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag
      961 gcattctgta tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg
     1021 tatcccacta aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc
     1081 taactgtttt taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa
     1141 ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt
     1201 cgaatccgta acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag
     1261 tatagcatat attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa
     1321 taactgcccc tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa
     1381 aaaagatctt aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt
     1441 ttctttgtga taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt
     1501 ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta
     1561 ggaacttttt ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt
     1621 cctttcttca ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa
     1681 tgttcgtgat agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt
     1741 tctttaaagc ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg
     1801 atcattgttt ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca
     1861 tgaccgttca actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga
     1921 gaatagattt cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta
     1981 atgcccgcat cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg
     2041 gggagaacat tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt
     2101 gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac
     2161 acaaataaaa ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac
     2221 aaacctttcg tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat
     2281 aagaagagaa tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat
     2341 aattggcagt ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca
     2401 ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa
     2461 ctggtttttt cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg
     2521 gcatacgaat tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt
     2581 tattatgaca gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga
     2641 atattttaat gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa
     2701 atgaaggttg ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag
     2761 aaaacccaca acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta
     2821 tgctagtagg aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag
     2881 ctagagattc tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca
     2941 caaaaccttt cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg
     3001 agttggcaga agcaagagaa gaagcagtag tttcagatga tcattttgat atcgagacta
     3061 gtcatttcaa gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag
     3121 gactgacgcc taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat
     3181 tctctcggga gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga
     3241 cagtggacgc ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca
     3301 tccaaactgt ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta
     3361 tttgtatcag caactcttag ctttctgcgg aatggtcgcg ttgatcttat tgatcgttgg
     3421 agtttctttt acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt
     3481 tggatatgct gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt
     3541 tagtcaagat caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca
     3601 aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc
     3661 tttactgaat tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga
     3721 aaaattcaca tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt
     3781 gccatttaat ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa
     3841 tatcgataac agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag
     3901 tatcgttgca gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg
     3961 gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca
     4021 tgacaaagat gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa
     4081 agaatctcaa gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc
     4141 ttctcacgag atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata
     4201 caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga
     4261 gcgtctgatc cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat
     4321 cagtatggtc attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt
     4381 agaagctaaa gcagaagcag caggagatac attgacattg aaagccgttg atgatattga
     4441 tgtttacgct gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat
     4501 acagtttact gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat
     4561 catcacgatt gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga
     4621 tcgttattat aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg
     4681 tttaccaatc gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga
     4741 actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta
     4801 aaaaacaagc ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa
     4861 aatgattgat ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag
     4921 catcatgagt aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt
     4981 ggagtagctc ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat
     5041 tgtttcgctt ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca
     5101 actcagctgc cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca
     5161 aacgatcttg tttgatttct ttttccggta catccaacag gacatggtct ttaagattca
     5221 gataaggaaa catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa
     5281 gaacctcttt tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa
     5341 cggtcaagtg ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt
     5401 tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg
     5461 agcccaataa gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg
     5521 aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc
     5581 aagatatcac ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga
     5641 agaagcgctt cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa
     5701 agaaagagag gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga
     5761 ccagtcaggt gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa
     5821 aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg
     5881 ctagctaatt ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc
     5941 cgatcttgga cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc
     6001 aataaaaaag cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa
     6061 attaatcgag cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata
     6121 gaaaatgaat atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa
     6181 aatattttgt aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc
     6241 aagcttttga acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc
     6301 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
     6361 gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt
     6421 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
     6481 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
     6541 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
     6601 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
     6661 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
     6721 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
     6781 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
     6841 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
     6901 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
     6961 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
     7021 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
     7081 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
     7141 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
     7201 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     7261 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     7321 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     7381 ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     7441 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     7501 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     7561 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca
     7621 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     7681 ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca
     7741 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     7801 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     7861 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     7921 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     7981 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     8041 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     8101 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     8161 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     8221 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     8281 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     8341 aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc
     8401 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     8461 cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc atttatttta
     8521 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     8581 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     8641 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     8701 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     8761 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     8821 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     8881 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     8941 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     9001 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     9061 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcatagg aggctatgag
     9121 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     9181 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     9241 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     9301 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     9361 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     9421 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     9481 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     9541 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     9601 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     9661 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     9721 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     9781 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     9841 cattagaagg agcgatgcag aatggttcgg gtaaagggat tgaatatagg aagcggtaga
     9901 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     9961 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
    10021 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
    10081 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
    10141 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
    10201 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
    10261 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
    10321 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
    10381 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
    10441 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggttggc
    10501 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
    10561 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
    10621 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
    10681 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
    10741 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac
    10801 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
    10861 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
    10921 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
    10981 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
    11041 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
    11101 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
    11161 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
    11221 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
    11281 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
    11341 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
    11401 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
    11461 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
    11521 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
    11581 cttttttctg tcaatatatg aataagctct tgtttggttt ttgtagggaa acaatccaga
    11641 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
    11701 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
    11761 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
    11821 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
    11881 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
    11941 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
    12001 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
    12061 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
    12121 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
    12181 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
    12241 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
    12301 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
    12361 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
    12421 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
    12481 tctgaagaaa gaaaacagaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
    12541 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
    12601 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
    12661 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
    12721 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
    12781 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
    12841 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
    12901 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
    12961 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
    13021 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
    13081 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
    13141 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
    13201 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
    13261 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
    13321 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
    13381 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
    13441 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
    13501 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
    13561 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
    13621 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
    13681 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg
    13741 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
    13801 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
    13861 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
    13921 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
    13981 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
    14041 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
    14101 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
    14161 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
    14221 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
    14281 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
    14341 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
    14401 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
    14461 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
    14521 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
    14581 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
    14641 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
    14701 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
    14761 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
    14821 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
    14881 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
    14941 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
    15001 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
    15061 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
    15121 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
    15181 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
    15241 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
    15301 caagaaaaac atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt
    15361 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
    15421 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
    15481 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
    15541 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
    15601 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
    15661 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
    15721 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
    15781 aagatcgttc cttcggggta agcagaacca ttggcagagt caccagaagt aatgacatcg
    15841 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
    15901 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
    15961 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
    16021 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
    16081 cttttttatt tttat
//
LOCUS       DACEJR010000059        16075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEJR010000059 DACEJR010000000
VERSION     DACEJR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.059"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IW651_002084"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IW651_002084"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IW651_002085"
     CDS             complement(227..697)
                     /locus_tag="IW651_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0814802.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IW651_002086"
     CDS             complement(856..1314)
                     /locus_tag="IW651_002086"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0814803.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="IW651_002087"
     CDS             complement(1485..2630)
                     /locus_tag="IW651_002087"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0814804.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="IW651_002088"
     CDS             complement(2643..3560)
                     /locus_tag="IW651_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0814805.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="IW651_002089"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="IW651_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0814806.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="IW651_002090"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="IW651_002090"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0814807.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="IW651_002091"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="IW651_002091"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0814808.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="IW651_002092"
     CDS             7086..7967
                     /locus_tag="IW651_002092"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0814809.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="IW651_002093"
     CDS             7964..8680
                     /locus_tag="IW651_002093"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0814810.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="IW651_002094"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="IW651_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0814811.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="IW651_002095"
     CDS             9397..10398
                     /locus_tag="IW651_002095"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0814812.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="IW651_002096"
     CDS             10395..11756
                     /locus_tag="IW651_002096"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0814813.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="IW651_002097"
     CDS             11716..12867
                     /locus_tag="IW651_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0814814.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="IW651_002098"
     CDS             12842..14011
                     /locus_tag="IW651_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0814815.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="IW651_002099"
     CDS             14186..15565
                     /locus_tag="IW651_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0814816.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16075
                     /locus_tag="IW651_002100"
                     /pseudo
     CDS             15846..16075
                     /locus_tag="IW651_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5257 a         2839 c         3428 g         4551 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacca
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atttgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DACEJR010000060        15990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEJR010000060 DACEJR010000000
VERSION     DACEJR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.060"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            226..894
                     /locus_tag="IW651_002101"
     CDS             226..894
                     /locus_tag="IW651_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0814817.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IW651_002102"
     CDS             complement(1094..1480)
                     /locus_tag="IW651_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814818.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IW651_002103"
     CDS             complement(1452..2834)
                     /locus_tag="IW651_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0814819.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IW651_002104"
     CDS             complement(2990..4177)
                     /locus_tag="IW651_002104"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0814820.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IW651_002105"
     CDS             complement(4184..5215)
                     /locus_tag="IW651_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0814821.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IW651_002106"
     CDS             complement(5231..6685)
                     /locus_tag="IW651_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0814822.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IW651_002107"
     CDS             6860..7765
                     /locus_tag="IW651_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0814823.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IW651_002108"
     CDS             7856..8533
                     /locus_tag="IW651_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0814824.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IW651_002109"
     CDS             8814..9710
                     /locus_tag="IW651_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0814825.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IW651_002110"
     CDS             complement(9765..11135)
                     /locus_tag="IW651_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0814826.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IW651_002111"
     CDS             11373..12119
                     /locus_tag="IW651_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814827.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLPFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IW651_002112"
     CDS             12116..12955
                     /locus_tag="IW651_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0814828.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IW651_002113"
     CDS             12956..13906
                     /locus_tag="IW651_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0814829.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IW651_002114"
     CDS             14047..14799
                     /locus_tag="IW651_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814830.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IW651_002115"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IW651_002115"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0814831.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5190 a         2998 c         3220 g         4582 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacaaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat cttccctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca
//
LOCUS       DACEJR010000061        15885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEJR010000061 DACEJR010000000
VERSION     DACEJR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.061"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            49..489
                     /locus_tag="IW651_002116"
     CDS             49..489
                     /locus_tag="IW651_002116"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0814832.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            617..1300
                     /locus_tag="IW651_002117"
     CDS             617..1300
                     /locus_tag="IW651_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0814833.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1609..1682
                     /locus_tag="IW651_002118"
     tRNA            1609..1682
                     /locus_tag="IW651_002118"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1643..1645,aa:Arg,seq:tct)
     gene            1841..2200
                     /locus_tag="IW651_002119"
     CDS             1841..2200
                     /locus_tag="IW651_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0814834.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2323..3954
                     /locus_tag="IW651_002120"
     CDS             2323..3954
                     /locus_tag="IW651_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0814835.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4016..4657)
                     /locus_tag="IW651_002121"
     CDS             complement(4016..4657)
                     /locus_tag="IW651_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0814836.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4939..5247
                     /gene="rplU"
                     /locus_tag="IW651_002122"
     CDS             4939..5247
                     /gene="rplU"
                     /locus_tag="IW651_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0814837.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5274..5618
                     /locus_tag="IW651_002123"
     CDS             5274..5618
                     /locus_tag="IW651_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0814838.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5631..5924
                     /gene="rpmA"
                     /locus_tag="IW651_002124"
     CDS             5631..5924
                     /gene="rpmA"
                     /locus_tag="IW651_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0814839.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6331..9165
                     /locus_tag="IW651_002125"
     CDS             6331..9165
                     /locus_tag="IW651_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0814840.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9356..9847
                     /locus_tag="IW651_002126"
     CDS             9356..9847
                     /locus_tag="IW651_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0814841.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9950..10939
                     /gene="manX"
                     /locus_tag="IW651_002127"
     CDS             9950..10939
                     /gene="manX"
                     /locus_tag="IW651_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0814842.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10972..11775
                     /locus_tag="IW651_002128"
     CDS             10972..11775
                     /locus_tag="IW651_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0814843.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11796..12710
                     /locus_tag="IW651_002129"
     CDS             11796..12710
                     /locus_tag="IW651_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0814844.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12793..13167
                     /locus_tag="IW651_002130"
     CDS             12793..13167
                     /locus_tag="IW651_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0814845.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13306..13551
                     /locus_tag="IW651_002131"
     CDS             13306..13551
                     /locus_tag="IW651_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0814846.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13723..14787
                     /locus_tag="IW651_002132"
     CDS             13723..14787
                     /locus_tag="IW651_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0814847.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15033..15458
                     /locus_tag="IW651_002133"
     CDS             15033..15458
                     /locus_tag="IW651_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0814848.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15451..>15885
                     /gene="nusB"
                     /locus_tag="IW651_002134"
     CDS             15451..>15885
                     /gene="nusB"
                     /locus_tag="IW651_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0814849.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT         5076 a         2633 c         3470 g         4706 t
ORIGIN      
        1 agttctataa cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga
       61 atttttggtt tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa
      121 ggaaatctga tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt
      181 acatattatg aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac
      241 aaccacgaag atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta
      301 aaaaagagaa atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt
      361 tcaaaaacgg catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca
      421 ggaatcacag catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa
      481 tttttgtaag aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca
      541 tgaaacacga gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt
      601 gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact
      661 attggatttt atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga
      721 agaagctcat gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt
      781 tttacttgga cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc
      841 atcaagtgtc atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt
      901 cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt
      961 gactttgctt gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt
     1021 tatttttatg gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt
     1081 gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct
     1141 cccagatgaa gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct
     1201 tcgtgttgtg atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt
     1261 gatcttaatg acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg
     1321 tagataatcg attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt
     1381 aagtcaatta aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc
     1441 tcctgttttc aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt
     1501 gatttagtag ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg
     1561 acagaagcaa tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct
     1621 cagctggata gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga
     1681 cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat
     1741 attgtttgtt ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag
     1801 gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat
     1861 tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac
     1921 tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc
     1981 tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact
     2041 ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat
     2101 tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg
     2161 tgggctagct ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt
     2221 tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat
     2281 gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt
     2341 tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg
     2401 gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc
     2461 ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt
     2521 ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg
     2581 tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta
     2641 tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc
     2701 gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat
     2761 ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg
     2821 ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg
     2881 tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg
     2941 gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa
     3001 acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa
     3061 gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt
     3121 gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa
     3181 ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc
     3241 cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca
     3301 gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca
     3361 gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag
     3421 gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt
     3481 acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt
     3541 tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg
     3601 gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg
     3661 ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt
     3721 gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat
     3781 gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca
     3841 gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca
     3901 gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat
     3961 gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat
     4021 attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc
     4081 gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc
     4141 attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat
     4201 gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg
     4261 tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa
     4321 atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt
     4381 tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct
     4441 cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc
     4501 taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt
     4561 taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta
     4621 aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta
     4681 ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa
     4741 aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac
     4801 ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc
     4861 agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag
     4921 aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt
     4981 gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt
     5041 tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca
     5101 ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc
     5161 aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa
     5221 gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca
     5281 aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg
     5341 ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca
     5401 gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag
     5461 aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata
     5521 ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact
     5581 cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat
     5641 ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg
     5701 actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat
     5761 caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg
     5821 gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta
     5881 acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta
     5941 aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag
     6001 tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat
     6061 caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg
     6121 aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg
     6181 atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa
     6241 tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt
     6301 acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg
     6361 tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca
     6421 caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag
     6481 ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa
     6541 acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg
     6601 gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt
     6661 ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg
     6721 ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac ttattttgca
     6781 cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt
     6841 gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg
     6901 tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg
     6961 gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg
     7021 atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat
     7081 gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa
     7141 acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca
     7201 aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga
     7261 aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat
     7321 ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg
     7381 caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg
     7441 attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag
     7501 ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct
     7561 tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat
     7621 caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat
     7681 aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa
     7741 atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt
     7801 cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac
     7861 ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat
     7921 gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt
     7981 ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca
     8041 agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc
     8101 caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat
     8161 atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga
     8221 agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa
     8281 agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa
     8341 actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa
     8401 aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt
     8461 cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc
     8521 aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct
     8581 ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata
     8641 gctactactg ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt
     8701 attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa
     8761 actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct
     8821 gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca
     8881 caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg
     8941 atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa
     9001 aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt
     9061 ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt
     9121 ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat
     9181 acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg
     9241 tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt
     9301 cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga
     9361 tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc
     9421 gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt
     9481 gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt
     9541 gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt
     9601 attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt
     9661 gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt
     9721 aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat
     9781 tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa
     9841 ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt
     9901 attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat
     9961 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
    10021 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
    10081 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
    10141 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
    10201 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
    10261 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
    10321 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
    10381 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
    10441 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
    10501 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
    10561 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
    10621 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
    10681 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
    10741 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
    10801 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
    10861 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
    10921 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
    10981 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
    11041 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
    11101 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
    11161 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
    11221 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
    11281 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
    11341 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
    11401 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
    11461 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
    11521 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
    11581 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
    11641 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
    11701 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
    11761 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
    11821 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
    11881 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
    11941 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
    12001 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
    12061 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
    12121 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
    12181 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
    12241 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
    12301 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
    12361 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
    12421 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
    12481 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
    12541 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
    12601 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
    12661 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
    12721 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
    12781 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
    12841 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt
    12901 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
    12961 ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa
    13021 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
    13081 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
    13141 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
    13201 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
    13261 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
    13321 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
    13381 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
    13441 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
    13501 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
    13561 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
    13621 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
    13681 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
    13741 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
    13801 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
    13861 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
    13921 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
    13981 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
    14041 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
    14101 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
    14161 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
    14221 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
    14281 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
    14341 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
    14401 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
    14461 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
    14521 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
    14581 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
    14641 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
    14701 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
    14761 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
    14821 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
    14881 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
    14941 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
    15001 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
    15061 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
    15121 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
    15181 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
    15241 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
    15301 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
    15361 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
    15421 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
    15481 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
    15541 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
    15601 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
    15661 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
    15721 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
    15781 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
    15841 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aatta
//
LOCUS       DACEJR010000062        15807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEJR010000062 DACEJR010000000
VERSION     DACEJR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.062"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(60..344)
                     /locus_tag="IW651_002135"
     CDS             complement(60..344)
                     /locus_tag="IW651_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0814850.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(334..1068)
                     /locus_tag="IW651_002136"
     CDS             complement(334..1068)
                     /locus_tag="IW651_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0814851.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1197..1820
                     /locus_tag="IW651_002137"
     CDS             1197..1820
                     /locus_tag="IW651_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0814852.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            2012..3133
                     /locus_tag="IW651_002138"
     CDS             2012..3133
                     /locus_tag="IW651_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0814853.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3140..6268
                     /locus_tag="IW651_002139"
     CDS             3140..6268
                     /locus_tag="IW651_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0814854.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            6287..9481
                     /locus_tag="IW651_002140"
     CDS             6287..9481
                     /locus_tag="IW651_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0814855.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9627..10334)
                     /locus_tag="IW651_002141"
     CDS             complement(9627..10334)
                     /locus_tag="IW651_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0814856.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            11099..11497
                     /gene="spxA"
                     /locus_tag="IW651_002142"
     CDS             11099..11497
                     /gene="spxA"
                     /locus_tag="IW651_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0814857.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            11829..12482
                     /locus_tag="IW651_002143"
     CDS             11829..12482
                     /locus_tag="IW651_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0814858.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            12708..13706
                     /locus_tag="IW651_002144"
     CDS             12708..13706
                     /locus_tag="IW651_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0814859.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            13882..15690
                     /gene="pepF"
                     /locus_tag="IW651_002145"
     CDS             13882..15690
                     /gene="pepF"
                     /locus_tag="IW651_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0814860.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         5492 a         2647 c         3256 g         4412 t
ORIGIN      
        1 tcaggcaaaa acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt
       61 catatagatt caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt
      121 cgggtgagtt ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact
      181 tctgcatgga tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta
      241 ccattgttat gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc
      301 tgatctttac agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat
      361 ctctgcagtg tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc
      421 gtctaatttg ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt
      481 tgggtaaaca aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat
      541 ttctaagaac cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt
      601 acttgaaata tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag
      661 gtgtttatta gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac
      721 gactaagtca caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg
      781 gacagtcatt tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag
      841 acgaggttga agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc
      901 aactgcgcca tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa
      961 gtgagcaagc agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt
     1021 gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca
     1081 aaatgaaaaa aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt
     1141 gtcaaacctc tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt
     1201 tttatcgatt tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc
     1261 gctatgagaa taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc
     1321 gtacttggtg ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta
     1381 tggcaaaaga agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg
     1441 cttttccagt taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt
     1501 tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac
     1561 ttaaaggcgg aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat
     1621 atcaaggtcc gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg
     1681 gcgaaccaat cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg
     1741 aacgacgaac acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat
     1801 atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg
     1861 tcctgttcag ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt
     1921 tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa
     1981 taattagaag gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca
     2041 tatcggaaaa aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc
     2101 tatactagat atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata
     2161 tgataggagt gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg
     2221 gaatttaaag gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag
     2281 actttcagcg ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc
     2341 agaagcattt gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga
     2401 accaattgca gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt
     2461 agccaaggtg atagataaaa tgcaggagct atttgattca gaaaaaaaac aagtattgat
     2521 tagccatttt tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa tcgaagtagg
     2581 cgggttggat tcgatccctg gtgaactact tacaccgttt gattatgtag cactaggaca
     2641 tttgcataat caagctgctt tgagacagga taatgctcgc tatagcggtt ccccattgaa
     2701 attctcttta tctgagatca accaaaaaaa aggcgtatgg atcgttgacc tgacaacagg
     2761 cctggagttg aattttaggg agatcgaacc cctgcatgat attgaagaaa taacagcgag
     2821 tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc aatcgggaga attacatcta
     2881 tatctatctc actgatagaa cgatcatccc gaatatgatg aaccagcttc ggcaaatata
     2941 tcctcgtatt ttggggattg aacgggcaaa cggtcgagca gaacaaacaa agcgcaaacg
     3001 aaaagtacta gaaatgaaag atcctcaaaa attagcagaa gcatttttta gtgaaatgac
     3061 ggaagagcag ctaactgcaa accaagaaaa atggctcgga gaaacgttgc aagaaatcat
     3121 ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt ttggtccttt
     3181 tatcaatgag accgtagatt tttcacgttt gacagaagca ccgctttttt tgatcagcgg
     3241 gaagacagga gctggcaaaa cgacgatttt tgatgggatc accttcgcac tttttggtga
     3301 aacttccgga agacttcgct ctggaaaaga gatgcgttct ttgtttgcta caccagacga
     3361 agaaacaagc gtgacttttt cttttgaaca tcagcagatg agctatgaaa tcgaacgaaa
     3421 accagaacaa gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg cagccaaagt
     3481 aacgttgacc atttttgatg ggaaaggaaa agaaataaaa caaatcacaa aacgtacaga
     3541 agcagatcag ttgatcagag aactgatgaa tctcgatgcg aaacagtttt ctcaaatcgt
     3601 tttactaccc caaggagaat tccgtaattt tttggtttca tcaagcagtg ataaagagac
     3661 tgttttacgt catttattcg gtactcaatt tttccaacag ttcaatgagc gattaaaaga
     3721 aaaagcaaaa caacagcaac aaaaattgga tcatttagag caagagctgc atcttctgca
     3781 aaaaaggttt gttttagtca aagaagagga accagcagca gggtttgaag ccgttttagc
     3841 ccaatggaca aaccatcagg caatactcga aaaacaaatc acggaagaaa aagaagctct
     3901 aacggaactt caggaaaaac aaaagcaact agaaaattcc tactatcgtt ttgaaagctt
     3961 gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag caaaaggaat tagctgctca
     4021 aaaagacgaa atagaggaaa agaaacgatg gattgggtat tatgaattta cgcaacgatt
     4081 agtcgaacca atccagcgcc tacaagaaat aaaagttgaa aaaatagaac tgtcacaaaa
     4141 aagtagtgag caaacaaaga aactagcgat gactacgcag aattatgaag agtggcaaaa
     4201 aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc aaaaactgca
     4261 aaacaaacag atgatggtgc caattgtcga atccttacgt agtaaaaaac aagacgcaga
     4321 acaattaaca atttcattgg ataaagaaga attgaatgcc gagaagctgt atcaaaatct
     4381 cgctatctat caggaaagaa ctcgaattct tcaagaaaat ttactggatc aggaaaaact
     4441 gcaagaagat cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc agcaagaaat
     4501 agaaaaacaa aaagcaaaag acactgggtc gctcgcacag ataagagaag aactcaaaaa
     4561 agcaactgaa gaaataacaa aaaatagtca gctattgaaa gaacagacag aacaagcaga
     4621 acagctgaaa agccaatggg caaagcagca aattgctcgt cttcagcttt ttttgcgtcc
     4681 aggggagcca tgtcttgttt gtggatcaac ggaacacccc atacaacaat taaaacacga
     4741 acagccaaat aaggaagata tcttaaaagt agaacaacaa ctggcagaag ctgagtctgc
     4801 tgtagaaaga actcagcaaa tcattgccaa aagtgaagca catgaaaata atcttcaaca
     4861 acaattatca gatttagaac aaaaaatcat ggcaacgaat atacaattgg ctgaacaaga
     4921 agaacacttg caacaggctt tgaaaaaggc atttcctgag ataacagaac aaaaaacaaa
     4981 aacaactctt gtagtaatcg aacaatcttt agatagacga aaaagcaaga tggccaatgc
     5041 aagaatagag ctggaagaaa taagacaaca agcagaaaag ctcgaacaac aattagctgt
     5101 ctacaaacag cagcagacag aaaaaaataa tcagctccag caactgcaag gagaaatcaa
     5161 aagtttagaa caacaattgc aagatacgga tagtaactgt ttagaagagg agatacgacg
     5221 acttgaaaaa cagttagaag aagactcgaa tcaactagct gttgagcaga aagcaggagt
     5281 gcaattgttg caagatcttc atacgcttcg gacacagctt gatcttcaac atgaacaaat
     5341 agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa agttagaaag
     5401 ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc aagcacaata
     5461 taaagaaaac caacgtacag ttacttctta tcatgaagag caattagtga cagctgaccg
     5521 gctgcgtcaa ttagaaaata tccagctaac tgagacattc ccagatacag aagagatcag
     5581 ccaacagtta gaagaaataa gacgtatgat cgaaataaag caagaaaaga tatacggttt
     5641 acaagaacaa aaaattagta atcaaaaaat ctacgatgaa tgtctttcga tttatcacac
     5701 aagccagacg aaattagatg agcttacgca attacagcag ctttcgcaaa cattcaacgg
     5761 agaaaatcct aagaagacta gtttggaacg gtatgtgctt caggtttatc ttcaagaagt
     5821 gttacaagta gcaaatgatc atctccaacg tttaacgaaa aatcgttatc aatttgagct
     5881 agcggatacc attggcagtt atcgagggaa aaccggatta gaaatcaata tctatgatga
     5941 tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt ttattgcagc
     6001 actttcttta gcattagctc ttgccgatgt gatccagaca caagctggtg gtgtagcaat
     6061 cgatgcattg tttattgatg aaggatttgg gtcattagat gaagaagctt tggaaatggc
     6121 aatggaagct ttagaaacga tcgaaagtga aggaagaatg atcggcatca ttagtcatgt
     6181 aagagaattg aaagaacgag tattacaaca aataagaatt gatacagaag gtagcggaca
     6241 aagcaaaata cgctatcaac ttggataaaa agggagattg gtaactatga aatggagtat
     6301 tccagaaaaa gtcattgcac gtggacgtca atacttgcaa gaaggccgag tgttatcggt
     6361 tgtccctgac caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg aattgtatat
     6421 ggttgatttg gatggtacag caaaagagat agattactgc caatgtccct attgggagga
     6481 acataaatat tgtaagcata cagttgctgt ggaactttat ttacggtcga aaaatctatc
     6541 tcgtatcatg aaaaaagacc agccaatgct agaaccggta aaagcaacaa gtgagggaga
     6601 aatcattgca aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag cggtgcaacc
     6661 tttgattatt gaataccaag tagaaacgat tgagactaat cagtatcacc cagagctttc
     6721 tattttggca ctttacttgc gtatcggttc attggcatca ccgaaaaaaa catatatcgt
     6781 aaaaaacatc tatgattttt tacaagctta cacagaaaaa gaaacttaca ctgttaataa
     6841 acagtaccag tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa aaatactcac
     6901 gcatcttgca ggaagtgctc agacgcatca attattaggt acgaacggct tacaagtcaa
     6961 aggaaaacta gataaaagat atctgcttct cccagtagaa caagcccgct tcctgttaga
     7021 gcagatgaat cagactacac gtttgcatct gcagataaat gaacaaaacg atcaaggaat
     7081 acaattttcc gatcaaaaca aacctttatc attcaaagtg gaaaaatttg gagaagatta
     7141 tttgctgcaa gcattgaatg attttgattt tttctttatg cattatcagt ggggagttat
     7201 ccaagggaca ttttattctc tgacgaaata tcaacaaacg gtttatcaaa cttggaaaca
     7261 attgattcgc cgattggaga aaccagaagt agtgttcaag aaaaaagaat taccgtcttt
     7321 gtttaaagag attatccctc tgctgtcaga gatcggcgac gtagctattg cccctgcagt
     7381 agaagaggaa gtagcagata tccccgtcga atttattttc tttttccgaa aagcaaaagg
     7441 aaaaatccaa gctcgagtcg atttcgtcta tggcgaagtc atctattcga ctgacccgaa
     7501 gcatgaggtc tcgacagaac aggcttttca ggtgttacgt gacacagtag aagagaaacg
     7561 agtacttgat ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt atgaacgtcc
     7621 attgccaagc ggggaagaac tatatgcctt tttccgcaca gaactatctg tcttccgtca
     7681 gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg tatttagatg cgcataaaca
     7741 tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc gatattcgat tcgatgtgac
     7801 agggatagag gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa atgatgcttt
     7861 ttatacatta gaaaatggtc aggttctttc ttttgattca gaagaatttc aacaaacaag
     7921 ccaagtgttg caacaattac gggaatcgat acgtacagaa gaaggaacga tccatgtacc
     7981 taaaaatcaa ggattgatca ttcaaaatca attggaaaaa agcaatgcta cttttagtga
     8041 atcgttccaa acgatggtac aagatctcat tcatccagaa agataccagg cacagcttcc
     8101 caaaggattg aatgcaacga tgagagatta tcaaaagcaa ggttttcgat ggttgaagat
     8161 gttaggtcat tatcagtttg gcggtatttt agccgatgaa atgggacttg ggaaaacact
     8221 acagacaatt gcctttttac tttcggaaaa agaagaacgg aaaagcttct ctgctttgat
     8281 cgtagcgcct gcgagcttaa tttacaactg gcaggcagag gtgagaaaat tcgccccttc
     8341 tttaagtatc caagtaatca atgggaataa aaaagaacgt gaagaattac tggctaaaga
     8401 cacagatatc cgagtgactt cttatgccag cttaagacag gatctagcta attatcagtc
     8461 gcaaaaaatt gattatttga ttcttgatga ggcacaaatg gtcaaaaata gcagtacgaa
     8521 aactgcacaa gcattgagag aattagctgt acctcagcgg tttgcactga gtgggacacc
     8581 aatcgagaat aatttagaag aattgtggtc attgtttgca acgattatgc caggattttt
     8641 cccgactaaa acaaaattca gggaacttgc cgcagaagaa attgcgcaaa tgattcgacc
     8701 gtttatttta cggcgagata aacaaactgt gttgaaagac ttgccggaaa aaacagagat
     8761 gaatctatat tctgcactta cagaagaaca aaaaactgtt tatttagctt acttgcgtca
     8821 aatgcgtgaa gaaatcacat caatggattc tgaagcattc aagaaaaacc ggatcgggat
     8881 acttgctggt ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt ttattgaaga
     8941 ttaccaaggc ggttccggca agctcgaaca agtaaaagat ctactggtag ctgccaaaga
     9001 gaacaaacga agagttttgc tgttttctca atttacaggg atgctgacta tcttgcaaga
     9061 agagttagcc gagctaggga tctcgacatt ttatttaaga ggcagtacga aaccacaaga
     9121 tcggttaagt atggtagacg catttaatgc tggcgaaaaa gatgtttttc tgatttcctt
     9181 aaaggctggg ggaactggat tgaatctcac gggagcagat acggtcatct tgtatgacct
     9241 ttggtggaat cctgccattg aagaacaagc tgctggacgt gcacatcgga tcggacaaaa
     9301 aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg attgaggaac gcatggattc
     9361 actccagcaa gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg aagaacagtt
     9421 gacgaaactg actgaagaag acatccgcat gattttaagt gtaggagaaa tcgaggagta
     9481 agagactcga aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa gcgattgttt
     9541 atttgattta aaaaatagca aggaggctgg gattccattt tatcccagct tccttgctat
     9601 cattttttta tttctatttt taaaggttat acaaagattg tcttcattgt ctctaacagc
     9661 tgatcaaaag aagaaatctc gatatcgcaa attggagtct gttcaatttt gcgttctcga
     9721 tggttgaacc aaagcgcttg ccagttagca ctttttgcac ctagaacatc attgtcgtaa
     9781 ttatcgccaa cgtataaggt ctcttctgga agcatatgaa attctttctc agctagctga
     9841 aaaatctctt tttctggttt ttggaaacct gttgcttgag aaatgagcat attgcgggaa
     9901 ggaatccaat tgttcaattg aagttgcttc agtttttttg tctgatggtc agtcggccca
     9961 tttgtgataa ttcctaatgg gacattcttt tccttcaaga aatcgagggt ttttttgacc
    10021 tcctcatgca agcagatatt atcaagttct tcctcgtaaa ttttttgaaa taagaggcca
    10081 tcttcttccg taatatgagg gtagtctaaa tcttttagtg attggctgat ccgatgtgct
    10141 cgcatatatt ctagtgtcca atctcctgcc attacttttg ggaagttttc atcactatga
    10201 tggcggaacc ggatatatag tggatgcata tctgcatctg aaacaagcgg aacgactctt
    10261 ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac atcaaacaca
    10321 actgatttaa tcaaaatttt acacgccttt cggttttcat tgattttttc acaaatcctt
    10381 gactattgta acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt ttcttactat
    10441 atttctattt ccttgacaga ataaggtttt tatatttcag tttttttcac taaaaatcgt
    10501 gaaacacgtc aataagtttg atgaacaaat taatcattat ggtagaagtc gattgattta
    10561 ttttaatgca atctctgtgg aattttcgaa agatttattt ttttaataca gtctcaatat
    10621 aactattcaa acataaaata acgttatttg atttatttgt ttatagtaaa ttttatttag
    10681 taatcattgc tttaaaatac tacgcttata taacagagaa aataagcgtt ataataaaga
    10741 acagtattat ttaaaatgac agaaaacatt actattaaag aaataaaaaa cataaaaaat
    10801 cttaagaaat cggtttcatt tagcttttga gataaaataa tagtacagtt ttatacgctg
    10861 tttcatatgt ttagaaaatt tgaattagat ttgaaaactc taatttttaa atgataaaaa
    10921 aagcgagaat gctggtttta ctgtatatta accttagata actttcattt ttttaaaaaa
    10981 attatgaaaa aaaggtagct atttaaaagt tgattatgta taatagaaac agatgggaga
    11041 ttattatgat cttctgttac aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt
    11101 gttaacatta tatacttccc caagttgtac ttcctgcaga aaggctcgtg cgtggttgca
    11161 agaacatcag attccatttg ttgaacgtaa tattttttca gaaccattga acagttctga
    11221 attaaaagcg attttacaaa tgacagaaga cggaacagaa gaaatcatct cgacacgttc
    11281 caaagtattc caaaaactga acatggattt agatgatctg ccgttacaag aattattgga
    11341 acttgtgcaa aacaatcctg gattgcttcg ccgtccaatc atgattgatg ataaacgtct
    11401 gcaagttggt tttaatgaag atgaaattcg tcggttccta ccgcgcgatg tacgacaatt
    11461 agaattacgt caagcacaat tgatggctgg attatagaag gataatacga aacttggaaa
    11521 agactattac aaagatagtt tttccaagtt tttttgtttt tacaaaaagt attcttgatt
    11581 agatgtataa aaaggtagta gaagatgatg attgcacgaa aaatagagag ggaaaattag
    11641 ctgaaaagtc ggctatatag tattcgtctg ccaaaaatgt tatagttaaa taagatagct
    11701 tgaaaggttg aaaaatccgc tggttttatt aaattcgctt aacagcgaga ttttttttct
    11761 ttacatttaa gctgatttcg ctacaatgaa tctatattaa acattgtggc aaagtgaggt
    11821 gtagcaaaat ggaaatggaa catattaatg aaaatacgat ccgtgtacta attggtcatg
    11881 aagatttaga ggaacggggc gtttcatttt tggatttact tggcaatcac aaagaaatcg
    11941 aaaatttctt ttatagtatt ttggaagaag ttgatatcga agaggagttc cgaggaagcg
    12001 aagctgtcac tttccaagtt ttgccaaaag gagacggatt agaactgttt atcagtaaaa
    12061 atcttccgga tgaagaaatt tctcaatcag aagattctac tgttgattca gaagatgtga
    12121 gtgactttct tcgtcagcag attattggca gcgatccgga ggaagaatta tctggaagcg
    12181 ccctagaaga acaggaaaga caagcaactt ttgcttttga agattttgaa caagtgatcc
    12241 aacttgcaac agatgtcgaa ctagagtctg cttggactga tttgtattta ttcgaagagc
    12301 gctattacct agtcgttcac ttctggatgg aaaatctcaa ccaagcggat gtagagaatc
    12361 agattgctcg aattttggag tttgctgaaa agagcgatcg tacaccagaa acattgaatg
    12421 aatatggaca atgtctgatg gcgcgtaatg ctatcgagcg aacgaaattt tattttgtat
    12481 aaatcagcat tatagtaaga gactgtgaca aaaattgtgt cgcagtcttt ttttcgtata
    12541 aatgtgttca aaagcttgca tctgcggtaa caagcccaac caaacgaaaa atattgggaa
    12601 ctatttttgt ttagctgttc ttattacttg atgcagaata gctttttcac gccctctttt
    12661 ttcgtgtaaa taaattctta gacgtaaata agttcagagg gagaaaaatg ctgcttgcaa
    12721 tgaatgaata taataagtgc tgttttgcta aacatgcaga aaaaggaaag aaatattatt
    12781 gtcgtggctg tcgaaaaaga gtgattttga aaaagggaaa gaaaaaatgt gctcattttg
    12841 cccatcaaaa atcagataac tgttcggtct ttagtgaaag agaatcaaaa gagcacttac
    12901 agttaaagga atgcttcatg gattggctgg gacagtcagt agaaccagta tttttggaag
    12961 cctatttgcc gaggttgcgg caaaggccgg atattttgtt ggcgaatcta gcaatagaaa
    13021 tccaatgttc tagactgtct caccagagat ttattgaaag aacccaaaat tatttgaata
    13081 acagttacca agtatggtgg atacttggta attcttttct tggacaatcg caattctcat
    13141 tgatagaaaa aagttgctgc tactataata gaaaaagagg agtgcattgc tggaaagctg
    13201 atctcaagaa acaaaagctt tatttgtatc atcatattac agagactgta tcagggcata
    13261 tttccttttg cagtagctgc tggacttttt ctagtaggga ccttaaagaa atattcacta
    13321 gtaacgaaat taaaatgaac caaatgaaaa aaatagaaaa agtgagtgaa gatgggaaga
    13381 aatggctgac acgccaactt attcacaagc aaaagaatac agtatccata caagaacaat
    13441 gctacttgcg tcataaacat cttttgcatc tatcatcgtg gatctatcaa aaaagtcggt
    13501 ttttctttta tttacaagaa caggtttttc tttatcgaat gttgtacgaa gaagccttgg
    13561 aaaaccagaa agttcctgat ttcaactcat ggctttgcca aataaaagaa cataaaaggg
    13621 aatggctttt tcctatgatt gatgaagaga tggtctatca gcagtttttt aacgaatgca
    13681 tacacttgtc ttctttgaaa aaatagtaag tataaaaata ggctaggaat cggttttcta
    13741 tgtaaaacaa gatttagtta tgctaaaatt gatggaagca gacacatgaa tgagtagaaa
    13801 agttagatgc tgtgacatgt ggatgttaga ctatgatcta ttttgataga tagagctaag
    13861 ggaagaaaag gagtggattt catggaagta aagcagttgc caaaacgaga agaattgcct
    13921 gaaaatttaa cttgggactt gaccaagatc ttttcaagcg accaagagtt tgatgagaaa
    13981 tatttggaat tatcagaaga gttaaaacaa tctgaaaaac acaaaggaac acttgatcaa
    14041 ggcgcttctc aatttttaaa tgcgattgaa ttcgtattga gggtttatcg ccaaactgaa
    14101 gtcatttatg tatatgcgca ccttaaaaac gatcaagaca ctggaaatac agattaccaa
    14161 gcgctttatg caagagcaag cagtctgttt tcgaaagtta gtgaagccgt ttcctggttt
    14221 gaaccagaaa tattgcaatt gtcagatgac cagatttggc aatatttcaa agaagaacca
    14281 aaattggaag tctatcgcca ttatatccag caaatagtag ataatcgagc ccatgtctta
    14341 tctgctgagc aggaatctct tcttgctgga gcaggtgaaa tctttgatgc ttcaagtgat
    14401 acatttgctg ttttgaataa tgcagatcta gtttttccaa cgattgaagg agaaaatggt
    14461 gaaatagtcc aattatctca tggcgtgtat ggtcagttgc tagaaagcac ggatcgaagg
    14521 gtgcgcgaag cagcatttaa ggggttgtac agtgtttacg aacaatttag aaatacattt
    14581 gcttctactt taggcacaca tataaaagga cataatttta aagcgaaagt ccgtaattac
    14641 agctctgcca gagaagcgtc tttgagcaat aatcatattc ctgaaagtgt atacgatact
    14701 ttggtagacg tggtaaacaa gcatttgcct ttgttacatc gatacatgga attacggaaa
    14761 cgtttattag aagtggaaaa actgcacatg tatgatcttt atacaccggt cttaggggaa
    14821 gctccaatta cctttacgta cgaagaagca aaagaaaaag ctttagaagc actgaaacca
    14881 atgggtgaag aatacatggc catcgtagaa aaagcattct ctgaacgttg gatcgatgtt
    14941 gtcgaaaata aagggaaacg aagcggtgct tattcttcgg gaagctatga cacaaatcca
    15001 tatattttat tgaattggca tgatacgctg gatcagctat ttacgcttgt ccacgaaatg
    15061 ggacatagtg ttcatagtta tttcactcgt tcgaaccagc cttatgtgta cggcgactac
    15121 tccatctttt tagcagaaat tgcttcgaca acgaatgaaa atatcctaac ggagtattta
    15181 ttggaaacag aaaaagaccc tcgtgtacgg gcttatgtac tcaaccatta cttggatggg
    15241 tttaaaggaa cagttttccg ccagacacaa ttcgctgaat tcgagcattt catgcatacg
    15301 gaagatgaaa aaggtgtgcc attgactagt gaatacctaa gtgatagtta tggtaaattg
    15361 aatgcaaaat attatggtcc agcagtcgaa gaagacccag aaattaaatt cgaatggtca
    15421 cggataccgc atttttatta taattactat gttttccaat actcgactgg cttttctgct
    15481 gcttcagcac tggcgaagaa aatactaaac caggaaccag aggcactaga gaactacttg
    15541 gcttacctaa aagcaggtaa cagcgattat cctgtggaag taatgaaaaa agcaggagtt
    15601 gatatgacac aggctgcata tattgaagat gcaatgtcga tgtttgaaca acgtctaaat
    15661 gaattagaag aattgatcga tcgtttataa ggctttctat ttttgatttt ctcgaataga
    15721 aacgaaaaaa atacgtcggc tctcttgtaa gggagtcgac gtattttttt aaggctctat
    15781 actttttgat gttggtgaac ttcacca
//
LOCUS       DACEJR010000063        15687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEJR010000063 DACEJR010000000
VERSION     DACEJR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.063"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW651_002146"
     CDS             <1..111
                     /locus_tag="IW651_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0814861.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="IW651_002147"
     CDS             124..510
                     /locus_tag="IW651_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814862.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1061..1285
                     /locus_tag="IW651_002148"
     CDS             1061..1285
                     /locus_tag="IW651_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814863.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1303..1731
                     /locus_tag="IW651_002149"
     CDS             1303..1731
                     /locus_tag="IW651_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814864.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1709..3127
                     /gene="terL"
                     /locus_tag="IW651_002150"
     CDS             1709..3127
                     /gene="terL"
                     /locus_tag="IW651_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ0814865.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3139..4674
                     /locus_tag="IW651_002151"
     CDS             3139..4674
                     /locus_tag="IW651_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0814866.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4754..5632
                     /locus_tag="IW651_002152"
     CDS             4754..5632
                     /locus_tag="IW651_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0814867.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5634..5951
                     /locus_tag="IW651_002153"
     CDS             5634..5951
                     /locus_tag="IW651_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814868.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6000..6701
                     /locus_tag="IW651_002154"
     CDS             6000..6701
                     /locus_tag="IW651_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0814869.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6716..7606
                     /locus_tag="IW651_002155"
     CDS             6716..7606
                     /locus_tag="IW651_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0814870.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7629..7844
                     /locus_tag="IW651_002156"
     CDS             7629..7844
                     /locus_tag="IW651_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814871.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7856..8197
                     /locus_tag="IW651_002157"
     CDS             7856..8197
                     /locus_tag="IW651_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814872.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8197..8568
                     /locus_tag="IW651_002158"
     CDS             8197..8568
                     /locus_tag="IW651_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814873.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8561..8956
                     /locus_tag="IW651_002159"
     CDS             8561..8956
                     /locus_tag="IW651_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0814874.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8958..9335
                     /locus_tag="IW651_002160"
     CDS             8958..9335
                     /locus_tag="IW651_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814875.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9336..9944
                     /locus_tag="IW651_002161"
     CDS             9336..9944
                     /locus_tag="IW651_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0814876.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9944..10285
                     /locus_tag="IW651_002162"
     CDS             9944..10285
                     /locus_tag="IW651_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814877.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10527..13316
                     /locus_tag="IW651_002163"
     CDS             10527..13316
                     /locus_tag="IW651_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0814878.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13306..14046
                     /locus_tag="IW651_002164"
     CDS             13306..14046
                     /locus_tag="IW651_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0814879.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14043..>15687
                     /locus_tag="IW651_002165"
     CDS             14043..>15687
                     /locus_tag="IW651_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814880.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPK"
BASE COUNT         5631 a         2186 c         3268 g         4602 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
      721 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      781 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      841 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      901 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      961 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
     1021 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
     1081 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
     1141 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
     1201 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
     1261 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
     1321 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
     1381 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
     1441 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
     1501 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
     1561 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
     1621 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1681 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1741 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1801 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1861 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1921 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1981 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     2041 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     2101 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     2161 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     2221 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     2281 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     2341 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     2401 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     2461 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     2521 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     2581 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     2641 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2701 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2761 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2821 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2881 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2941 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     3001 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     3061 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     3121 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     3181 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     3241 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     3301 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     3361 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     3421 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     3481 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     3541 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     3601 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3661 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3721 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3781 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3841 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3901 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3961 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     4021 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     4081 ctgaaagacc ctaatgcgaa agccggctac gtgacaaagg atctacaaaa ggatttcaac
     4141 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     4201 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     4261 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     4321 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     4381 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     4441 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     4501 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     4561 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     4621 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4681 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4741 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4801 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4861 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4921 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4981 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     5041 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     5101 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     5161 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     5221 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     5281 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     5341 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     5401 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     5461 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     5521 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     5581 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     5641 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5701 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5761 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5821 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5881 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5941 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     6001 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     6061 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     6121 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     6181 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     6241 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     6301 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     6361 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     6421 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     6481 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     6541 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     6601 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6661 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6721 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6781 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6841 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6901 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6961 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     7021 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     7081 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     7141 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     7201 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     7261 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     7321 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     7381 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     7441 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     7501 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     7561 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     7621 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7681 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7741 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7801 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7861 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7921 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7981 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     8041 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     8101 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     8161 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     8221 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     8281 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     8341 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     8401 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     8461 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     8521 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     8581 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     8641 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8701 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8761 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8821 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8881 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8941 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     9001 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     9061 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     9121 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     9181 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     9241 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     9301 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     9361 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     9421 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     9481 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     9541 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     9601 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9661 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9721 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9781 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9841 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9901 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9961 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    10021 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
    10081 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
    10141 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
    10201 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
    10261 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
    10321 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
    10381 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
    10441 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
    10501 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
    10561 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
    10621 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    10681 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    10741 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    10801 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    10861 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    10921 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    10981 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    11041 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    11101 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg tctcaaggta
    11161 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    11221 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    11281 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    11341 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    11401 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    11461 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    11521 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    11581 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    11641 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    11701 tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag gttatatcta
    11761 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    11821 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
    11881 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    11941 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    12001 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    12061 gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca ttgattacag
    12121 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    12181 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    12241 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    12301 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    12361 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    12421 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    12481 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
    12541 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
    12601 ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg gcatacaata
    12661 cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag gactctttct
    12721 ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat ctaaaaaatg
    12781 gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg gaagtcaatt
    12841 tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa gaaaagtgga
    12901 atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg attaaaggag
    12961 ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa aatattgttc
    13021 aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc atgacaaatt
    13081 tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta ccaattacac
    13141 aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat ttaaatgttt
    13201 atggagattt acctgattca atggttcgga agattaccac aaaaattaaa acagaattga
    13261 caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca acttaagaga
    13321 ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat tcaaaacagg
    13381 cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg aaatgattct
    13441 atcattattg ataaaggcta ttacagaaat gtgactagaa agattgagtg ttactataaa
    13501 gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa tcactgaatg gttagatatg
    13561 tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca agctgtagta
    13621 atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc ttttgaattt
    13681 acagtgagca ttcgaccatt taaggaaaat tacaatggta gatttactat tcgacaaaca
    13741 gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat taaattgagt
    13801 ggttctggag atgcttcttt ttatattaat aaggacaaat atgatttgaa atcgttggac
    13861 agagaattat ttatagattc taaattagaa gaggcttacc gaaaattaga tggtaattta
    13921 gaacatcagg atcaagtcac tttattttta gattttccat ttttatatcc tggaaaaaat
    13981 gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa
    14041 gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa
    14101 ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag
    14161 agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa
    14221 aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag
    14281 atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac
    14341 gggtagtcac tcttgcggaa gtagactcta aaactggtca agaagcgata tcaattattg
    14401 aaaccaaaat ggataaaaaa tctgacatac gactttattc tgatattacg gccgtttcta
    14461 gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc
    14521 ttcaatattg gggtggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa
    14581 gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt ttaaaggtca
    14641 aattagattg gacaggtata aaaacaagac ttattccata tgctgatcct caaagtgagg
    14701 taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc
    14761 ctgatgtgta tacagagcat gttcagttta cagaagaaca aggagtaaaa gacattaaga
    14821 gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta
    14881 aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg
    14941 cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata
    15001 tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga
    15061 aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta actattcagg
    15121 ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt
    15181 caatgattac aggtcaaggc agtgcaggag gatatgtaag tttatggcca aaagaaaaac
    15241 cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa
    15301 tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt acttcagctt
    15361 ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc aaagcagata
    15421 tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat ggactactcc
    15481 aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg gaattttgga
    15541 attctaaggg ttcaattgga acgattggaa caactgattc tgctggtaat ccttttcctg
    15601 gggcttctac tccaacccct attgaagata attctttagt tattcgtaca aatggagacg
    15661 gcaaatatat tttgatttct cctaaag
//
LOCUS       DACEJR010000064        15525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEJR010000064 DACEJR010000000
VERSION     DACEJR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.064"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..808
                     /locus_tag="IW651_002166"
     CDS             68..808
                     /locus_tag="IW651_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0814881.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IW651_002167"
     CDS             complement(1232..1402)
                     /locus_tag="IW651_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0814882.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IW651_002168"
     CDS             complement(1596..1775)
                     /locus_tag="IW651_002168"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814883.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1785..2009)
                     /locus_tag="IW651_002169"
     CDS             complement(1785..2009)
                     /locus_tag="IW651_002169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814884.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2162..3274
                     /locus_tag="IW651_002170"
     CDS             2162..3274
                     /locus_tag="IW651_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0814885.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IW651_002171"
     CDS             complement(3352..3645)
                     /locus_tag="IW651_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0814886.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IW651_002172"
     CDS             3813..4127
                     /locus_tag="IW651_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0814887.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IW651_002173"
     CDS             4148..6127
                     /locus_tag="IW651_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0814888.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPSVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IW651_002174"
     CDS             6140..6466
                     /locus_tag="IW651_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0814889.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IW651_002175"
     CDS             6482..7000
                     /locus_tag="IW651_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814890.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IW651_002176"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IW651_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0814891.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IW651_002177"
     CDS             complement(8436..9941)
                     /locus_tag="IW651_002177"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0814892.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IW651_002178"
     CDS             complement(10033..10623)
                     /locus_tag="IW651_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0814893.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IW651_002179"
     CDS             10802..12448
                     /locus_tag="IW651_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0814894.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IW651_002180"
     CDS             12497..13216
                     /locus_tag="IW651_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0814895.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="IW651_002181"
     CDS             13695..14642
                     /locus_tag="IW651_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0814896.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..>15525
                     /locus_tag="IW651_002182"
     CDS             14654..>15525
                     /locus_tag="IW651_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0814897.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPF"
BASE COUNT         4778 a         2678 c         3164 g         4905 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 ctctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca ttttt
//
LOCUS       DACEJR010000065        15325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEJR010000065 DACEJR010000000
VERSION     DACEJR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.065"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IW651_002183"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IW651_002183"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0814898.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IW651_002184"
     CDS             951..1256
                     /locus_tag="IW651_002184"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0814899.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IW651_002185"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IW651_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0814900.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IW651_002186"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IW651_002186"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0814901.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IW651_002187"
     CDS             4416..5354
                     /locus_tag="IW651_002187"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0814902.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSAEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IW651_002188"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IW651_002188"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0814903.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IW651_002189"
     CDS             6725..7465
                     /locus_tag="IW651_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0814904.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IW651_002190"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IW651_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0814905.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IW651_002191"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IW651_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0814906.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IW651_002192"
     CDS             10608..11258
                     /locus_tag="IW651_002192"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0814907.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IW651_002193"
     CDS             11259..11897
                     /locus_tag="IW651_002193"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0814908.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IW651_002194"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IW651_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0814909.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IW651_002195"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IW651_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0814910.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..15248
                     /locus_tag="IW651_002196"
     CDS             13674..15248
                     /locus_tag="IW651_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814911.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5142 a         2874 c         3327 g         3982 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggctgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcag
    14701 caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca acaacacctg
    14761 cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca ggaagtagta
    14821 ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca cctgctccag
    14881 ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa tatattggta
    14941 ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg ttcacacgct
    15001 atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct caagaatcag
    15061 ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc tgggggtcac
    15121 aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc catgctcctc
    15181 aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac tttgctgtca
    15241 gcatgtaaga atgattaatc aaaaagagtt agcccattct ggactaactc tttttttatt
    15301 gtgataaaat agaaatcaga taaat
//
LOCUS       DACEJR010000066        14966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEJR010000066 DACEJR010000000
VERSION     DACEJR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.066"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..183)
                     /locus_tag="IW651_002197"
     CDS             complement(<1..183)
                     /locus_tag="IW651_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0814912.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLP"
     gene            complement(370..1020)
                     /locus_tag="IW651_002198"
     CDS             complement(370..1020)
                     /locus_tag="IW651_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814913.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1563..2498)
                     /gene="whiA"
                     /locus_tag="IW651_002199"
     CDS             complement(1563..2498)
                     /gene="whiA"
                     /locus_tag="IW651_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0814914.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2532..3530)
                     /locus_tag="IW651_002200"
     CDS             complement(2532..3530)
                     /locus_tag="IW651_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0814915.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3527..4411)
                     /gene="rapZ"
                     /locus_tag="IW651_002201"
     CDS             complement(3527..4411)
                     /gene="rapZ"
                     /locus_tag="IW651_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0814916.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4546..5277)
                     /locus_tag="IW651_002202"
     CDS             complement(4546..5277)
                     /locus_tag="IW651_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0814917.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5281..6546)
                     /locus_tag="IW651_002203"
     CDS             complement(5281..6546)
                     /locus_tag="IW651_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0814918.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6809..9625)
                     /gene="uvrA"
                     /locus_tag="IW651_002204"
     CDS             complement(6809..9625)
                     /gene="uvrA"
                     /locus_tag="IW651_002204"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0814919.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9635..11629)
                     /gene="uvrB"
                     /locus_tag="IW651_002205"
     CDS             complement(9635..11629)
                     /gene="uvrB"
                     /locus_tag="IW651_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0814920.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11888..13390
                     /locus_tag="IW651_002206"
     CDS             11888..13390
                     /locus_tag="IW651_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0814921.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13392..14129
                     /locus_tag="IW651_002207"
     CDS             13392..14129
                     /locus_tag="IW651_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814922.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14174..14347)
                     /locus_tag="IW651_002208"
     CDS             complement(14174..14347)
                     /locus_tag="IW651_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0814923.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14323..>14966
                     /locus_tag="IW651_002209"
     CDS             14323..>14966
                     /locus_tag="IW651_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0814924.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
BASE COUNT         4377 a         3217 c         2550 g         4822 t
ORIGIN      
        1 aggcaagtta attttcataa ccttatgggg gattatccaa ttaaaaatcg gaaaaaggta
       61 gcctccccaa aaagtatagt gatctgtgtc aatcagataa taacctgatt ttgattccag
      121 aataacatat ctcaaagccg aacgaaaatt tttcatgaat atccataatc tcactgtttc
      181 cacacctaat tcctccaatt ccaattaaat ggatttatag catcaccaat tatgttaact
      241 gcctgaccaa cctcattgat aaaattggca ccactatccc atgcttcaac aatgctatat
      301 atctatcaaa caaaaaatgg aaaactaaat actcgcgcac ataaatttta cattctgttt
      361 tttataagac taataataat cattgaaaaa atttcttatc ggtggcgcat attcccaatt
      421 gattaatgtg atcccatatt ctgcaagtat tttacgagct tcttctaagt catagtgaga
      481 aaaatgcata aaacctgcaa tacgatcacg gactgtgagc tgtggcttgc ttccaccatc
      541 attcataaaa tatccagcag agccactact taaagtgatt gttacatctt cactttctat
      601 tattaagaat tcataatgaa atgctttcaa ctctccacta ttatttaaaa gttttatact
      661 ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa cttcctataa attctgcaat
      721 caccgtatct tcaatcaaaa ttctttcctc atctatttca tattcaagac taaacggaaa
      781 ttctccatat gtaatctctg gcaatggtgg tgctgtagtt ctccaaataa atacaataac
      841 tgtgaataga aaaaatccaa cgatcgatat aaatgttatg agacctgctc gaagtaaaac
      901 ccgatataat aaaaatgctg tatttcgctt tgctatatct agaaaaataa aaagtactgc
      961 aatcataatg atagtaaaag ttgtaaaaaa gcacattttt tgccctccct ttataaacat
     1021 cgattctcaa attctaaacc aaatcttgat agttcaagtt caggtataac gtataccaac
     1081 catacatccc aattactatt tatgaactta tgattatcac tgaataaatc actccatccc
     1141 cttgttaaaa cttctacatt aattacacct ggttgttttc gactagctaa tccaatgaac
     1201 ttatcaacca tccttatcca ccaaccacga gaatcttgac cagaagtaag aaaaacagtt
     1261 ctcaaggttg atcgagcagt tccttccagt ttttaattaa tctccagtta tagataaata
     1321 cttgatccta tctatatcag gacttctata ttaagcaaaa gtttatgtat aaaactaaaa
     1381 tcttcattca gttgtctccc cgctttttat acgattgtat taaaaaacaa tagaagcctt
     1441 tcaacattat gttactccag taaagttgga attatagttt gacggaagaa aagcaagtat
     1501 agcttttaaa aaaaactgat acattcgcat ctcctgcttc atgtatcagt tttttctttg
     1561 aattaaactg cttcttcacg cagtttttcc gcaaattcat ttattttgcg aattcggtga
     1621 ttaattcccg attttgatat tgcacctgag ggaatcattt cccctaattc tttcaagctg
     1681 acctctggat gttgaagccg aagttctgcg atttcctgaa gtttttcagg aagggcatgt
     1741 agaccgacct ttgcttcgat caattcgatg ttttcgattt gtttagaagc cgcatcaatc
     1801 gtcttgttca gatttgctgt ctcacaattg actagacgat tgaccgaatt tctcatatcc
     1861 cgtacgattc gaacatcttc aaatcggagc attgagttgg ttgcaccaat caatgttaag
     1921 aaatcagcaa ttttttctgc tccttttaaa tacgaaatat agccattgcg ccgttcaagc
     1981 gttcgagcat gtagaccgta ataatttaac atatcgcaaa tgtcttggtt atgctgttca
     2041 taaatagaaa atatttccaa atgataacga ctcgtttccg gattattgac tgaacctgag
     2101 gccataaaag caccacgtaa gtaagaacgc attttttgcg aatttcccat aatctcgtcc
     2161 gaaacgtgtc cattgaacat cataccatcc atgatatcaa gatccatcaa cacatctttt
     2221 gtgccttgct tcaaccggac gatatagaca ttgttctttt ttaatttcat ttttttacga
     2281 acgagtagct cacttcgtac ctgataatga tctttcaaaa gagaatagat acgtctggca
     2341 attgcagcat tttctgtttg gacgttcaaa ataaactgct ggttcacaat acctaaagag
     2401 ccgttcattc gcagcaaagc agctaattcc gctttcgcat gttctcgatg aacttccaaa
     2461 ccagttagtt ctttcttcac atctgcagca aatgacatcc tattctcctc ctttctccaa
     2521 caataaattg cttaatattt tgaaccgaaa actaaacgga acaattcttc tactactttg
     2581 tctccatcgt ggaagacacc accatctttc aattctaaaa agtcggtgga aatcactcga
     2641 catccttcat ttctcaatcc gttgaagtcg tgtgcgactt gtactagata ctcatcatag
     2701 atctctgggt ccatataatc ttcaggcact ttttccgtat tgaccaatac tgtatcgata
     2761 aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat cggcatctgt gaaatgttcc
     2821 gtttcccctt tttgggtcat gatattacag atatagacgg tttcagcagt tgtcttgagc
     2881 attgcttcgc cgatttctgg tatcactaaa tttggaagga tactggtgaa taaacttccg
     2941 ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa cgacttttcg agccgctttg
     3001 gcttcacttt tatcatttgt gttgcagaca tacacacggt caatggtttt acggttgatc
     3061 gcgattttag attctcctac tgcagtcgtt ccgtctttaa aaacagcatg caaggtcaaa
     3121 ggagtttctg aagaaggata aacatgtcca tctacatgca tcatttttgc taataattga
     3181 atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg caatgatcag attaccgatc
     3241 gtatgattgg ctaaaaactg atcttccttt ttgaaacggt attggaaaat atcttcatag
     3301 aattgtggca tatccgataa cgccactaag acatttctca agtcaccagg aggagccata
     3361 gcgatcgatt ctctgattgc accgctgctt ccaccatcat ctgcaacagt cactactgct
     3421 gtgatatctg ctccttgatt tcgtaaactt ttcaggataa ctggcagacc agtgccaccg
     3481 ccgaccacta cgatctttgg ttttcttatc cggtaggttt tcattttcac gaccggttca
     3541 ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt gactttatag tttttcccta
     3601 aagcatttgc caaacgttca gtcaatgcca cagaacggtg ttgaccgcct gtacagccga
     3661 ttgcaactgt caagctgctt tttccttctt taatgtatcc aggcaataca gtttccagaa
     3721 gatccaaaaa tttatggtag aatgattgcg tttcatcaaa ggacatcaca taatcataga
     3781 ctggctgatc ttttccggtc aatggtctta gttccgggat ataatgaggg ttgggcaaaa
     3841 aacgcacatc catcacaata tccgaatcga tcggcaaccc gtatttaaaa ccaaatgata
     3901 caaattcgat ccgaaatcct tggtctccct ttgatttgaa tgactcattg attttttcac
     3961 gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac ggaagcttgg gctttcaagt
     4021 cgtctagtat cgcccgttct ttacgtatcc cttcagtgat caaaccgtcc atcgccattg
     4081 gatgtgctcg tcttgtttct ttgtagcgag ccactagttc ttcgtctgaa gcatctaaaa
     4141 ataagataga agtgtcgatg aagttcgtat tttcgatttc gatcagcatg ttttggattt
     4201 cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc catttttgtg actttacctg
     4261 attctttgat caattcccag aattttggaa tcaatcgagg cggcatatta tcgatacaaa
     4321 aatagcccat atcttcaaaa ctttggatag ctactgtttt ccctgcacca ctcatacctg
     4381 ttatgatgac taattgcaaa ttatctgtca tatttacgaa cccctctcac attgtgcata
     4441 ataaaattat atcatcccgg gcgtatcatt accagttttc tttttctttt acaaaaagat
     4501 ttggaccaaa tatcttttag gatatttgcc ccaaatccaa tttttttact tttccgatgc
     4561 tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg atcgttcgat ccgctaaaat
     4621 agattgagca tcgatcactg ggtaatgatt atcttcagca aattcatgca tcaaagataa
     4681 ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc tctaccgctt cctctaagat
     4741 ttcgtaatag agctcttgat tcaaataatc cgattccttg atctcatgat agatcaggta
     4801 attgattttt tcttgaagcg cagtatcagg aatcactgtt tcgaaaccaa gatttttgac
     4861 ttcgcgttca tagattccag ctttcatact tccttctgtt cctagaatcg ctactctccc
     4921 tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt ggcatatgca agataggaat
     4981 atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg gtgttacaag tcaacacaat
     5041 gaagtttggt cgcaataaat tttgtttctc aatatcatcc agcagaaacg gcattggatt
     5101 ttcttctgat cgatctaaaa tataagctgt acgatcagga actgttgcat gattgaataa
     5161 gacataattc aaatattctt gatcttttgt agctttcgtt cggtggttga tcaagcgaac
     5221 aaaactttct gtcgccatcg tgcccattcc gcctaaaata ctgaaaaaat tctccatgaa
     5281 ctagccttct ttcacatgaa aatacttttt gaatcttgga atatagttat ggaacatata
     5341 tttcatcaac aaccagcgct tgattgatcg gtctttttca taaaatacag tagtaccgta
     5401 acgattttct ttgatcattt gctcagctag ttttttgtct tcattttccc gagcatattc
     5461 taaaaagatt tttttgggca cacccatcca taatcttgct ttatctgatt gattcgttcc
     5521 ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct tctgtgacaa aacgagccag
     5581 attcaagccg ttcaatgtca caaagaaact gctacgacct tgtctcaaat tgatttcaaa
     5641 taatttatat tctccatctc taggatcata cttcatatcg aaattggcaa agcctgtata
     5701 ttcgattttt tccaaaaatg ccttaatggt ttgatagatt ttttcgttat aatctggcat
     5761 gatcacaacg taattaccaa tcgatgctgg tgtaggatct tctaatagtg gatgaccaag
     5821 gcacatcatc cgaacttgat gatcttcatc tacatacgca tttagcacac gcatattact
     5881 atcatcaccc gggataaaat cttgtacaat catttcactt ttataaccgg cttcgtatat
     5941 ccggcctaaa attaaatcga actcttctct agtctctaaa ataaaagctt tttttcttcc
     6001 ttcaaattgg acagaaagat actccacact gtttgcaggt ttcaaagcaa ctgggaattc
     6061 aaaaggcaat tcctgttcca aatgtccatt tactaacatt tcttctctga cgatcaatgt
     6121 tttaggatac ggaagatcat attcttcaca aacttcataa aagcttactt tattgatcag
     6181 cctttcaaat aaagaataat caatatatgg acagataaaa tattctgaca gctcttgttt
     6241 atgttgggag atcaattctg catatccatc accacaagca atcaaaagat attttttact
     6301 cttatcagaa tatctttctt ttgccaaacg aagcatcgtc tcgataaata cagggtcttt
     6361 atcaaatcct ggaataactt ctacattgac aattttactg tagcgggtag gggctaactg
     6421 atccgaagca tatgcttggc agattttgcc gtatgcttca ttaaaagaac gtgccatacc
     6481 ataaacgttc atgtcgcttc ctaataatat agggataaat tcttcatttt caatactgtt
     6541 catcattatc tctcacttac tcataatctc ttgtttttct tttccaacta cacaaggaat
     6601 atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc cgattgctca attctttttt
     6661 gaacggattg aacttatagg aagtttttat ctataccaat cagtgtagaa agaactacca
     6721 tgtaatgatt ctagaacact gatattaata aaacaagcaa aaattcattt ttttatgttt
     6781 ttttaactat ctcagctatc tatcctcttt ataccattat ttttttcaaa tagtgacctg
     6841 tataactttc ttttacgtta atgatttctt ctggtgttcc agtcgcaagg atcgttcctc
     6901 caccttctcc accttctggt cctaaatcaa tgatataatc tgctgttttg attacatcta
     6961 gattgtgttc aatcactaaa actgtgttac cagcatctac taatctttgt aatacatgca
     7021 acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg ttcatctagt atatagaaat
     7081 tctttccatt agagattttg tgcaattcac tggcaagttt catccgttgt gcctcaccac
     7141 cggacaatgt cgttgctggt tgccccatcg tcacataacc taagccaaca tcaacaatcg
     7201 tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa ttctacagca tcctctactg
     7261 tcatttccaa aatatcagca atgctttttc ctttgtaatg cacttctaat gtttcagaat
     7321 tatatcgttt accatgacat acttcacaag gaacatagac atcaggcaaa aagtgcattt
     7381 cgatcttaat aattccatcc ccgcgacaag cttcacaacg accgcctttt acgttgaaac
     7441 taaaacgccc tttcttataa ccccgcattt ttgcctcgtt cgtttgagca aataacccac
     7501 ggatatcatc aaatacactt gtataagtcg ctggattact tctcggcgtc cggccgattg
     7561 ggctttgatc gatatcgatg atcttttcga tactttcgta gccggaaatt gtcttgaatt
     7621 tacctggctt agcagaattc ttatttaatt tttgcgctaa cgatttcttt aagatactat
     7681 tgaccaatgt actctttcca gaaccagaca cacctgtgac tgctacaaat tctcctaatg
     7741 gaaattcaac actaatattt ttcagattgt tttctgaagc accagtgatc ttgattgctt
     7801 tcccattacc tttacggcgt tcttttggta cagggattga ttttttccct gaaagatatt
     7861 tccctgtcaa agaattttca tcattagcta cttcttcagg agtgcctgca gccacgattt
     7921 gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa ataatctgct gcccacatcg
     7981 tatcttcatc atgttcgact acaacgaggg tgttgccaag atcacgcatt ttctttaatg
     8041 agccaatcag tcgatcgttg tcgcgttgat gcaatccgat cgatggttca tctaaaatat
     8101 acaaaactcc agaaagattc gatccaattt gtgtagccaa tctgattcgc tgcgcttctc
     8161 ccccagataa tgttccggaa gcgcgactca aagttagata gtctagtccc acattttcca
     8221 agaatgaaag tctgtcattt acttctttta aaatcggttt ggcaattact ttctcttgtt
     8281 cagataacgt taagtttgag aagaaatcta ctgctttttg aatagacaac tcatttgttt
     8341 gcccgatatt cgttccattg atttgaacag atagagcttg gggattcaat cgataacctt
     8401 tacatgtctg acaagttaat tctgtcatat aaagacgcat ttgctcacgc gtgaaatcac
     8461 tgttcgtatc atgataacga cgctcgatat tcttcaatac cccttcgaaa gtcgtttcta
     8521 catcgcgaac gccaccaaaa tcattttcat aatggaaatg gaaaagatca tctgtcccat
     8581 tcaagatgat ctcctgatgc tcttccggta attcttcgaa tggtgtatct aaatcgatac
     8641 cgaaactttc acaagcctgc gctaacattt gaggataata ctgagagctg attggattcc
     8701 atggaacgat agcaccttga ctaagtgtct tcgttctatc tggaatcact aaatcttgat
     8761 cgacttctaa tttgacacct aaaccatcac agtctggaca agctccaaaa ggcgcattga
     8821 atgagaacag tcttggttct agttcgccaa cagtaaatcc gcaataagga caggcatagt
     8881 gttcgctaaa caacatttct tcttcaccga tcacgtcaac cagagcatag ccgtctgcta
     8941 atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg aatcccttct ttcacaatga
     9001 tccggtcgat gacgatcgcg atatcatgtt ttttgttttt ctccagctca ggcacttcac
     9061 ttaaatcata gatctcacca tcgactcgaa cgcggacgta tccctctttt tggattcgtt
     9121 cgaatatttt tttatgttgt ccttttttct ttaccacgat cggtgccaac acttggatct
     9181 ttgtgcgctc aggcaaagcc attacttgat ctgccatttg atctactgat tgactggtaa
     9241 tctcgatatg atcgttggga caaatcggat gtcctacacg tgcaaacaac aagcgtaaat
     9301 agtcattgat ttccgtcaca gtacctactg tcgaccgagg atttttactt gttgttttct
     9361 gatcaataga aatcgccgga ctcaatccgt cgatgctatc tacgtccggc ttatccattt
     9421 gtcctaaaaa ctgacgagca taagcagaaa ggctctctac gtatctccgc tgcccttcag
     9481 cgtacagcgt atcaaatgct agggaacttt ttccagaacc ggacaaacct gtaacgacca
     9541 cgaatttatc tcgtggaatc gttacatcta tatttttcaa gttatgggca cgtgcaccat
     9601 gaatcgtaat tttatcgtta gccatgttgt cctcctactt tgaagctttt aattctaaaa
     9661 tcatatctcg cacattcgcg gctgtttcaa aatccaaagc ttttgccgca tctttcattt
     9721 ctttttctaa tttcatcaat aacgtgtctt tttcttcctt cgtcatttct tcgtacgaga
     9781 cttgtaccgc ttcttttgta tcatcttccg attctttcgt aatagaaatc aaatctcgga
     9841 tttctttaat gatcgttttt ggaacgatcc catgttcttc attgtacttc tgctgaatcg
     9901 tacgacgtct tgacgtttca tccatcgcta gacgcataga atctgttact ttgtctgcgt
     9961 acatgattac tttaccttct tcattccgag cagctcgtcc aatcgtttgg actaaggaac
    10021 gttcactacg taaaaagcct tctttatccg catccaagat agcaacaagg gaaacttcag
    10081 gtacgtctaa tccttcacga agcaaattga ttccaactaa cacatcaaat tcacctaatc
    10141 gaagatctcg aatgatttct gttcgttcca gcgttttgat atcactatgc aaatatttga
    10201 ctttgatccc taattctttg aaataatctg tgaggtcttc tgccattttt ttcgtcaatg
    10261 tcgttacaaa cacccgctgg tctttttcga ctcgttcatt tatttcgcct actagatcat
    10321 cgatttgtcc catgattgga cgaacttcga tcactgggtc aagcagccct gtcggacgga
    10381 tgatttgttg gataacggta tctgtttgtt catgttcata tggtcctggc gtcgcagaga
    10441 cataaatgat ctgattgaca tgtttttcaa actcttctaa ccgaagtggc cgattgtcta
    10501 aagcagatgg caaacggaaa ccgtaatcta ctagcatctg ttttctcgct cggtcaccat
    10561 tatacattcc tctgatttga ggcatggtta cgtgcgattc gtccgccacg atcaaaaaat
    10621 cttcaggaaa gaaatccaat agcgtataag gcggttcacc ttctttacgt ccgtccatat
    10681 gccgagaata attttcaatt cccgacgtgt agcccatttc taacatcatt tcaatatcgt
    10741 aattggttcg ctgttctaaa cgttgagctt ccagaagttt gttttcattt cgcaagacag
    10801 ttaaacgctg ttctaattcc gctttgatat tcgctacggc atgctccata tgttcatcat
    10861 tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc cgtttctcct aaaacttcac
    10921 ctgtcagtgc attgacttct cggatacgtt cgacctcatc tccaaaaaac tccacacgta
    10981 aggcacgttc atctcttgaa gccggaaaaa tctccacgac atcaccgcgc actcgaaaac
    11041 gaccgcgctg aaaatctata tcatttcttt cgaattggat actgactaag tcacgaatca
    11101 actgattgcg atctagttct gctccctgtc gaatcgaaac tacttgtttt tggtactcaa
    11161 aaggcgagcc taatccaaaa atacatgaaa cggatgcaat gacgatcaca tcatttcttt
    11221 ctaataatga actagtagcc gaatgtcgta atttgtcgat ctcatcattt acacttgaat
    11281 ctttttcgat atacgtatcg cttgaaggca cataagcttc aggctgataa taatcataat
    11341 aagaaacaaa atattcaaca gcattgttag ggaaaaattc cttgaattcc ccgtacagct
    11401 gaccagccaa tgttttgtta tgggcaataa tcagtgttgg tttattcact ttctcaataa
    11461 gattggaaat cgtatatgtt ttccctgttc cagtcgcacc taataagatt tgtgcttttt
    11521 tcccgccgac tacgccatcc actaattggt taatcgcttc aggctgatca ccagccggtt
    11581 ggtatttcga gacaagctca aagtgtcggg aggtatctct ctcaatcatt cttcgtcctc
    11641 cttttcattc attccttttc attttacgac caataaaaag aaaaggaaag caaaggcatt
    11701 ccttactaaa aaattatacc acaccgaaca tactttcgcc acttttttat cttattatac
    11761 atgttatgtt ttataacata aaatgttaga aactcgcatt tttttcattt tttcgagctt
    11821 acagtcttgt ctgattcagc acttttcgcc tataataata aaatattgga aatttaggag
    11881 gttgattatg aaaagaaagc atttatttca ctcatttgtt ttcttaatga caggacttgt
    11941 gacacttgcg tttggtcatg ttgcacaagc tgacaacact acaacggcag agtcaagcac
    12001 aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta tacgtaattg ccagtgactc
    12061 tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag tacgaaggca tcgatgtaaa
    12121 attgatggaa aacatcgcca aactgcaagg atttaacatc cgaatggact accgtggttt
    12181 ctctgctgct ttgcaagctt tggaatcagg acaagcagac ggaatgattg ccggaatgac
    12241 tgtaacagat gaacgaaaga aaagctatga tttttctgat tcttactttg atagcgggat
    12301 ccaattagct gtcaaaaaag gagatacttc gatcaagtct tatgaagatt tgaaaggaaa
    12361 aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt ttaagcgacc atgaatctga
    12421 atatggctat tctatcaaat attttgatgc tgctgatcaa ttgtacgacg ggttaaaagt
    12481 tggcgcaatc gatgcaatga tggatgatta tccggtaatc ggttatgcga tcaaacaagg
    12541 acaagaattg gaaacaccga tcaaaaaaga atctggcgga aaatatgcat ttgccgttaa
    12601 aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa ggattgaaag aattaaaaag
    12661 aacaggcgaa tacgataaga ttattaaaga atatgtagcg gatggttcag aaacttccag
    12721 tgagtcttcc actgctgatg aatcaacatt tcttggactt atccaaaaca actatcaagc
    12781 attgttgaaa ggactttgga gaacaattct cctaactttg atctcctttg ctttggctat
    12841 agtaatcggt gtgatttttg gattattcag tgtagcacca atacgcgctc ttcgcacaat
    12901 tgctagtatt tatgtcgata tcatccgagg aattccaatg atggttcttg cgttctttat
    12961 tttctttggg ttgcctggta tcgttggatt tacgattcct gacttccttg ctggtgttat
    13021 caccttgact cttaacgcaa gtgcttatat cgcagagatt gttcgaggcg ggatcaacgc
    13081 cgttcctgtc ggacaaatgg aagcttccag aagtttaggt ctttcttata atcggacaat
    13141 gcaaaaaatc attttgccgc aagcgatacg catcatgatc ccatcattcg tcaatcagtt
    13201 cgttatctca ttaaaagaca caacgatcat ttccgcaatc ggtgtagtcg aattgctgca
    13261 aactggtaag atcattgttg cacgtacaac acaaagtacg tatgtttact tgatcattgc
    13321 gatcatgtac ttgattttga tcacagcatt gacaaaatta gcaaaagttt tagaaaagaa
    13381 ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt agtcaaaaaa tacggcgata
    13441 acacagtatt aaacgacatc aatgtatcca tcaacgaagg agatgtcgtc tgtatcattg
    13501 gcccttcagg ttcaggaaaa agtacattct tgcgttgctt gaatcgttta gaagaacctt
    13561 ctgacggtga aattattatt gacggcgccc acttgatgga taaaagcaca gatatcaact
    13621 tggtgagaca acatatcgga atggtattcc aacactttaa cctattccct catctttcta
    13681 ttttggaaaa catcatttta gcaccgatgg acctgaagaa agaatctaga gaagaagcag
    13741 agaaaaaagc aatcaaatta ctgcagacag ttggtctaga agataaaaaa gatatgtatc
    13801 ctgaaatgct ttctggtgga caaaaacagc gtgtggctat tgctcgtgct ctagcgatga
    13861 accctgacat catgcttttt gatgagccta cttctgcact cgatcctgaa atggtcggtg
    13921 atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat gacgatggtt atcgttacgc
    13981 acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat gttcattgac gacggaaact
    14041 ttttagaaga tggaacgcct caacaaatct ttgaaaatcc gcaaaatgaa cggacaaaag
    14101 atttcttgaa taaagtatta aatatttaat aaaaaccaaa aatgcacggt cgccatgaag
    14161 ggaccgtgta tttttatctt cttattttct cacctaatcg tatcagccgc atcgcttgtt
    14221 ttcctgcaca aacaagcaat cgctctacct gttccatcgc ataagaaagt accatcggtt
    14281 cattgatgat atcaagtaca tagtcgatac caccctcact taattgagct tgatttttct
    14341 tctccatatt atgtagttgt gggacaactt tctgacaatc agcaattgtg tcaagaacgg
    14401 atcaatcctt tattggagtt gctcgaaagg gtcttctctt attacggaca ggctttgctc
    14461 actgtacata gacaacaaat cattatccta gtcaatcgga tttccagagc ctcattgcta
    14521 tcattgcttg ataaaatcca gacaaagttt aataaaatgt atcagttaca gctaaacttt
    14581 ggaattgggt cgctttgtta tacagagcaa gagacaccgc aatctttcct tcatgccaaa
    14641 caagtttgcg aatggatagc tttccatcaa tctgtgaacg agatccgctt ttttgaagat
    14701 ttagacctcg ggatcgtttt accagcaatc ccgtcagatc aacgaacatt atatgtaaaa
    14761 cgtattttga aaagcctgac agaagaagaa gtacatttgt tcaagaagac tttggcatgc
    14821 ttcagtaaaa ataacggaag catcaaaaat tgctcagaag aattatttat tcataaaaac
    14881 accttgcaat atcgattgaa caagttccat tctctcactg gttatacccc tagaaactat
    14941 gacgattatc atattctaaa gctggc
//
LOCUS       DACEJR010000067        14480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEJR010000067 DACEJR010000000
VERSION     DACEJR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.067"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW651_002210"
     CDS             complement(862..1203)
                     /locus_tag="IW651_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0814925.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IW651_002211"
     CDS             complement(1203..1433)
                     /locus_tag="IW651_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0814926.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IW651_002212"
     CDS             1720..2058
                     /locus_tag="IW651_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814927.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IW651_002213"
     CDS             complement(2263..2502)
                     /locus_tag="IW651_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0814928.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IW651_002214"
     CDS             complement(2478..2825)
                     /locus_tag="IW651_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0814929.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3557..4138)
                     /locus_tag="IW651_002215"
     CDS             complement(3557..4138)
                     /locus_tag="IW651_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0814930.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4321..4947)
                     /locus_tag="IW651_002216"
     CDS             complement(4321..4947)
                     /locus_tag="IW651_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0814931.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4969..6300)
                     /locus_tag="IW651_002217"
     CDS             complement(4969..6300)
                     /locus_tag="IW651_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0814932.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6455..6856)
                     /locus_tag="IW651_002218"
     CDS             complement(6455..6856)
                     /locus_tag="IW651_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0814933.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6942..7148)
                     /locus_tag="IW651_002219"
     CDS             complement(6942..7148)
                     /locus_tag="IW651_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814934.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7243..8745)
                     /locus_tag="IW651_002220"
     CDS             complement(7243..8745)
                     /locus_tag="IW651_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0814935.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8763..9218)
                     /gene="argR"
                     /locus_tag="IW651_002221"
     CDS             complement(8763..9218)
                     /gene="argR"
                     /locus_tag="IW651_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0814936.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9316..9933)
                     /locus_tag="IW651_002222"
     CDS             complement(9316..9933)
                     /locus_tag="IW651_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814937.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10154..11527
                     /locus_tag="IW651_002223"
     CDS             10154..11527
                     /locus_tag="IW651_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0814938.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11602..12714
                     /locus_tag="IW651_002224"
     CDS             11602..12714
                     /locus_tag="IW651_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0814939.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12786..14423)
                     /locus_tag="IW651_002225"
     CDS             complement(12786..14423)
                     /locus_tag="IW651_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0814940.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRFDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         4003 a         2992 c         2514 g         4971 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattccgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     3481 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     3541 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     3601 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     3661 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggat agtttttctt
     3721 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     3781 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     3841 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     3901 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     3961 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     4021 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     4081 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     4141 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     4201 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     4261 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     4321 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     4381 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     4441 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     4501 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     4561 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     4621 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     4681 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     4741 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     4801 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     4861 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     4921 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     4981 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     5041 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     5101 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     5161 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     5221 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     5281 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     5341 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     5401 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     5461 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     5521 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     5581 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     5641 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     5701 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     5761 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     5821 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     5881 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     5941 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     6001 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     6061 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     6121 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     6181 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     6241 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     6301 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     6361 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     6421 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     6481 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     6541 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     6601 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     6661 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     6721 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     6781 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     6841 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     6901 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     6961 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     7021 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     7081 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     7141 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     7201 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     7261 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     7321 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     7381 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     7441 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     7501 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     7561 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     7621 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     7681 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     7741 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     7801 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     7861 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     7921 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     7981 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     8041 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     8101 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     8161 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     8221 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     8281 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     8341 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     8401 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     8461 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     8521 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     8581 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     8641 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     8701 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     8761 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     8821 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     8881 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     8941 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     9001 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     9061 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     9121 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     9181 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     9241 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     9301 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     9361 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     9421 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     9481 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     9541 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     9601 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     9661 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     9721 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     9781 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     9841 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     9901 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     9961 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    10021 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    10081 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    10141 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    10201 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    10261 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    10321 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    10381 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    10441 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    10501 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    10561 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    10621 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
    10681 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    10741 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    10801 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    10861 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    10921 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    10981 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    11041 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    11101 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    11161 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    11221 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    11281 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    11341 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    11401 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    11461 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    11521 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    11581 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    11641 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    11701 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    11761 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    11821 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    11881 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    11941 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    12001 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    12061 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    12121 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    12181 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    12241 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    12301 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    12361 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    12421 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    12481 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    12541 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    12601 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    12661 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    12721 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    12781 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    12841 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaaatcg ttgtttcacc
    12901 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    12961 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    13021 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    13081 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    13141 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    13201 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    13261 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    13321 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    13381 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    13441 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    13501 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    13561 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    13621 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    13681 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    13741 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    13801 agcacaaccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    13861 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    13921 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    13981 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    14041 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    14101 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    14161 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    14221 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    14281 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    14341 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    14401 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    14461 ttattataca ttattcgagt
//
LOCUS       DACEJR010000068        14459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEJR010000068 DACEJR010000000
VERSION     DACEJR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.068"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            309..2780
                     /locus_tag="IW651_002226"
     CDS             309..2780
                     /locus_tag="IW651_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0814941.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2883..4619)
                     /locus_tag="IW651_002227"
     CDS             complement(2883..4619)
                     /locus_tag="IW651_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0814942.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4616..5320)
                     /locus_tag="IW651_002228"
     CDS             complement(4616..5320)
                     /locus_tag="IW651_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0814943.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5451..5954
                     /locus_tag="IW651_002229"
     CDS             5451..5954
                     /locus_tag="IW651_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0814944.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5914..6252
                     /locus_tag="IW651_002230"
     CDS             5914..6252
                     /locus_tag="IW651_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0814945.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6273..7691
                     /gene="ffh"
                     /locus_tag="IW651_002231"
     CDS             6273..7691
                     /gene="ffh"
                     /locus_tag="IW651_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0814946.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7803..8381
                     /locus_tag="IW651_002232"
     CDS             7803..8381
                     /locus_tag="IW651_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0814947.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8385..8906
                     /locus_tag="IW651_002233"
     CDS             8385..8906
                     /locus_tag="IW651_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0814948.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8985..9539)
                     /locus_tag="IW651_002234"
     CDS             complement(8985..9539)
                     /locus_tag="IW651_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0814949.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9792..10067
                     /gene="rpsP"
                     /locus_tag="IW651_002235"
     CDS             9792..10067
                     /gene="rpsP"
                     /locus_tag="IW651_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0814950.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10079..10330
                     /locus_tag="IW651_002236"
     CDS             10079..10330
                     /locus_tag="IW651_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0814951.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10455..11246
                     /locus_tag="IW651_002237"
     CDS             10455..11246
                     /locus_tag="IW651_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814952.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11416..11937
                     /gene="rimM"
                     /locus_tag="IW651_002238"
     CDS             11416..11937
                     /gene="rimM"
                     /locus_tag="IW651_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0814953.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11927..12688
                     /gene="trmD"
                     /locus_tag="IW651_002239"
     CDS             11927..12688
                     /gene="trmD"
                     /locus_tag="IW651_002239"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0814954.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12824..13171
                     /gene="rplS"
                     /locus_tag="IW651_002240"
     CDS             12824..13171
                     /gene="rplS"
                     /locus_tag="IW651_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0814955.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13429..13722
                     /locus_tag="IW651_002241"
     CDS             13429..13722
                     /locus_tag="IW651_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0814956.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4843 a         2596 c         2935 g         4085 t
ORIGIN      
        1 tgttaatccg agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga
       61 cgttttggtc gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa
      121 agtgttagta gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt
      181 tttttgctat atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca
      241 tatctgttct atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag
      301 gtagccacat gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat
      361 catcagataa agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag
      421 tcaaggaacg aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa
      481 atcagcaaat cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag
      541 ccattttgct ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag
      601 ccaatacagg aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa
      661 atgttttaaa tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata
      721 tagaagacct tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag
      781 atgcagaggt cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag
      841 atgaaataac caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa
      901 aagcttacgc ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca
      961 aagccaagac aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga
     1021 tcaaatggat tgggatcatc attattccta tcggtatcct gcttttttct caaagttact
     1081 tttttaatgg aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta
     1141 tgatacctga aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac
     1201 tagcaaagca aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa
     1261 atgtcctttg tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa
     1321 tgatcgtctc taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg
     1381 ctgactatgc aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt
     1441 ttacaaaaac cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat
     1501 tcagcagtgt agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc
     1561 gcgatcaatt gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg
     1621 tgttggtctt tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca
     1681 caccattagg ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg
     1741 attacttcaa aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg
     1801 tatccaatgt agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta
     1861 aattatcaga agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca
     1921 agccagaaca aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga
     1981 caggtgatgg cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg
     2041 cttccggaaa tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt
     2101 cacgcatgcc agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag
     2161 ccagcttgtt tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct
     2221 ttgctctgac ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg
     2281 gacttccatc tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca
     2341 tcatgaatgt gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg
     2401 cgttggtgat ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta
     2461 ccatgctttt gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata
     2521 aattcagaag tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt
     2581 tgcataagct gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc
     2641 tatttttctc ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg
     2701 aacgtaaaaa tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga
     2761 tatttaaacg agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact
     2821 cgtgtatctt tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt
     2881 ttttacattt tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca
     2941 gtaatcgtac cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc
     3001 ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc
     3061 tgatcctcaa tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt
     3121 gcctgatagc ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatc
     3181 agatttttga taattggata aaggatatct gttttagtag taaatggttc gttctttcca
     3241 atcaatcgaa tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc
     3301 tggaaataag gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac
     3361 agctggatga tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca
     3421 agaaaagaag tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa
     3481 ccaatcagtg aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt
     3541 tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg
     3601 gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta
     3661 agggtgatct cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg
     3721 agttttggct cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt
     3781 aaatgtagct gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg
     3841 aatacgccaa tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac
     3901 gcccgataca tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc
     3961 cattgatcgg gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt
     4021 agtacgggca gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt
     4081 cctgcaagaa caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca
     4141 gttctcggta aaaaaacgct tgtcggttct gcgatacgta aaatggcttc taactggcca
     4201 ctttttttga ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc
     4261 aaagcgctac ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc
     4321 tgttcattca atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa
     4381 gtaatccgtt cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct
     4441 tggaggtttt cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt
     4501 tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa
     4561 aacaaactaa ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc
     4621 gtttgggttc ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt
     4681 ttggatctcg ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt
     4741 gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt
     4801 cttttcgttt catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat
     4861 ggtcgtctac ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat
     4921 attcaggctc agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt
     4981 tcatacgggc taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta
     5041 tttctaggcc aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg
     5101 tttcattttt ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca
     5161 tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc
     5221 catctgtggc actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta
     5281 caatagaagg ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg
     5341 tttcattctg tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat
     5401 aaatggtata ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa
     5461 tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag
     5521 aattgatgga actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg
     5581 gattaccaat tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga
     5641 aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg
     5701 gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg
     5761 aaaaaagccc ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa
     5821 aaaatggtgc cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg
     5881 atgaagaatg gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg
     5941 aacgcgctct ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa
     6001 ttatattatg cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga
     6061 caagcagtat atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa
     6121 ctccatcttt tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat
     6181 attacagata agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca
     6241 atcgaagaat aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc
     6301 tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag
     6361 aaatgatgcg ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca
     6421 aagattttac taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat
     6481 cacctgcaca acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg
     6541 aaacagcaga gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc
     6601 aaggggcagg taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa
     6661 atgctcgtcc gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga
     6721 aagtattagg tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg
     6781 tagagatcgt ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga
     6841 tcgatacggc aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca
     6901 aagaattgac gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg
     6961 ccgtcaacgt agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta
     7021 aacttgatgg ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac
     7081 caatcaaatt catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc
     7141 gtatggctag ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc
     7201 aagactacga cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg
     7261 atttcaatga ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc
     7321 taatcaaaat gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc
     7381 caaaagatgt tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa
     7441 atcctgatct gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg
     7501 ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa
     7561 tgtccaaagg ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag
     7621 gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa
     7681 aacggaaata agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc
     7741 ttttctatca gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac
     7801 agatgagatg tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct
     7861 ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc
     7921 aagccggatt gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg
     7981 atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg
     8041 aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag
     8101 cagtcttgaa aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta
     8161 atggaaaaat cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc
     8221 tttcagatga attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa
     8281 caatctatgc ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc
     8341 gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat
     8401 tttatggtgc tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc
     8461 tttttgacac tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata
     8521 cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa
     8581 tttatccaga gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg
     8641 ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa
     8701 aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga
     8761 ttgatgaatg gtatatccag attacccctg tattattagg ggacgggatt cctttattcc
     8821 aaaaaggaac atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta
     8881 tcgaattgca ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag
     8941 aaatgtcctg tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg
     9001 ttagaaaaat ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg
     9061 atattaccat tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc
     9121 aaggttggca cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg
     9181 ccgatcccac ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt
     9241 ccataaggtc ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg
     9301 tattcaggag taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc
     9361 cattcagcag gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg
     9421 atttttacaa atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact
     9481 tttttgttgt aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata
     9541 cgatttttcc tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa
     9601 ataaaaacac ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa
     9661 gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa
     9721 aagctttaca gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt
     9781 ggaaataaat aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt
     9841 tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg
     9901 taggtactta caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc
     9961 ttgactggtt atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag
    10021 aaggcgttat gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat
    10081 gaaagattta agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca
    10141 agtcaagtta gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc
    10201 tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat
    10261 cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa
    10321 ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta
    10381 ttaattgaag aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag
    10441 ggagtgaaca gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg
    10501 cttgtagttc ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa
    10561 ctgttaccag tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg
    10621 ctacctcaaa taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg
    10681 ttcccatgcc tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata
    10741 caaagcaagg ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg
    10801 acgaattgaa tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta
    10861 gtcaagaaac aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg
    10921 acttaggttc tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat
    10981 ggcaagaagg aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct
    11041 tactattagc taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac
    11101 aatttggtaa aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct
    11161 ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga
    11221 tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact
    11281 ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat
    11341 accttaaaag aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag
    11401 aaatggggat ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt
    11461 atcaaaggcg aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa
    11521 ggaacggtgc taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt
    11581 catcgcaaac ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat
    11641 gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct
    11701 gaaaatgaat tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag
    11761 ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt
    11821 ccgaaaaaag ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta
    11881 gaagctggag tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt
    11941 attgacttta tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc
    12001 tgtagataaa ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa
    12061 acatcagaca gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca
    12121 gcccatctat gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt
    12181 aattcttctt gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca
    12241 agaagaacac ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac
    12301 attagtgaca gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc
    12361 gatggtcatg atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc
    12421 agctcaaacg gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc
    12481 agaatataaa gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga
    12541 agaatggcaa ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga
    12601 aacacttgaa tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca
    12661 agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga
    12721 taatatgtta gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt
    12781 gagaaaagct cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga
    12841 agaattaaca aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt
    12901 gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt
    12961 tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa
    13021 cggagtgggc gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt
    13081 agttcgctac ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa
    13141 agctgctcgt atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct
    13201 tggtattcaa gggttctttt ttcttagcat gtaatttgaa caccctgatc cagttttttc
    13261 tttaattatt ggtaggggag aataatggat tagaataaat ctacctataa gtagtagtaa
    13321 aaaaaagagc actatgataa tatctttctt aagagtaacc gctttaataa aaaatccaga
    13381 atattctaac ataaatggag tattcttaga atcaaaagga gaaagaagat ggtttatact
    13441 tatacggagc aagaaaagat gaaagtttta gacacattca tgaatgatgg aaaagtaaaa
    13501 caagtaccaa gttcaacaaa aaagaaatac atcctattga aagaagtctt gaaacgcttt
    13561 gaccatggag tgacttatac agaaacagag gttaattcga tactgctgaa cgtattttct
    13621 agtggcgatt atgtcgaaca aagacgttat ctaatcacat ttggcttttt caaaagaagt
    13681 tcagacggtc gggcatatca gatgatggga attgaaaatt agaagtttta atttatactc
    13741 attgaaaaaa gttcaagtat gcgatattgg ttctacaccg catacttgaa ctttttttgt
    13801 catctgtatc tataatacaa agccaagaac gttattcaaa atatggatag ctatgataaa
    13861 agctaatgag cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc caggagcgta
    13921 catgaaaaaa tcaattggat gcgttacatg aataaagata attctctttt atttgtacga
    13981 ataaatacca aaacgtacct cctaaaatta gattttggac agatgccctt taaaaagata
    14041 acaagatttc gttcttgaaa caatatatat gcttttattc caatgaggtt gtgaaaacag
    14101 tattttgtcc tgagcgttag aaaattgaaa atggggaata gcaactcgta ctttaccatt
    14161 ttttaagtga atacaagatc agctaattga acaccgttta ttcgtgatga gggacacaac
    14221 actgcagtcg acacatttct cttttatgtt caaagataac aaatatttaa aacaaagaaa
    14281 aatagtagat aatcccgtcc taactctttt attcttccct catagcttcc aaatttaagc
    14341 aatacttcaa ttaatcaagt tttatctttt tttttcaata atcgtaaaaa aagagtattt
    14401 tttatttaaa aataaaatct ttgtattaaa ggacaagact aaaataacct tagaataaa
//
LOCUS       DACEJR010000069        14406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEJR010000069 DACEJR010000000
VERSION     DACEJR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.069"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..627)
                     /locus_tag="IW651_002242"
     CDS             complement(52..627)
                     /locus_tag="IW651_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0814957.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(632..1606)
                     /locus_tag="IW651_002243"
     CDS             complement(632..1606)
                     /locus_tag="IW651_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0814958.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1630..1674)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1889..2857
                     /locus_tag="IW651_002244"
     CDS             1889..2857
                     /locus_tag="IW651_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0814959.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2970..3248
                     /locus_tag="IW651_002245"
     CDS             2970..3248
                     /locus_tag="IW651_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0814960.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3366..3602
                     /gene="secG"
                     /locus_tag="IW651_002246"
     CDS             3366..3602
                     /gene="secG"
                     /locus_tag="IW651_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0814961.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3714..4460
                     /locus_tag="IW651_002247"
     CDS             3714..4460
                     /locus_tag="IW651_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0814962.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4463..6820
                     /gene="rnr"
                     /locus_tag="IW651_002248"
     CDS             4463..6820
                     /gene="rnr"
                     /locus_tag="IW651_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0814963.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6842..7306
                     /gene="smpB"
                     /locus_tag="IW651_002249"
     CDS             6842..7306
                     /gene="smpB"
                     /locus_tag="IW651_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0814964.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7608..8327
                     /gene="atpB"
                     /locus_tag="IW651_002250"
     CDS             7608..8327
                     /gene="atpB"
                     /locus_tag="IW651_002250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0814965.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8380..8595
                     /gene="atpE"
                     /locus_tag="IW651_002251"
     CDS             8380..8595
                     /gene="atpE"
                     /locus_tag="IW651_002251"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0814966.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            8686..9210
                     /gene="atpF"
                     /locus_tag="IW651_002252"
     CDS             8686..9210
                     /gene="atpF"
                     /locus_tag="IW651_002252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0814967.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9197..9739
                     /locus_tag="IW651_002253"
     CDS             9197..9739
                     /locus_tag="IW651_002253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0814968.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            9762..11318
                     /locus_tag="IW651_002254"
     CDS             9762..11318
                     /locus_tag="IW651_002254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0814969.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            11332..12234
                     /locus_tag="IW651_002255"
     CDS             11332..12234
                     /locus_tag="IW651_002255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0814970.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12257..13663
                     /gene="atpD"
                     /locus_tag="IW651_002256"
     CDS             12257..13663
                     /gene="atpD"
                     /locus_tag="IW651_002256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0814971.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13677..14099
                     /locus_tag="IW651_002257"
     CDS             13677..14099
                     /locus_tag="IW651_002257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0814972.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4769 a         2580 c         3110 g         3947 t
ORIGIN      
        1 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
       61 ttttcttttt gaatatatca agcgttgtgg caacatagtg atgactaatg aaacaacgac
      121 gatagacaac aaacgatcaa ggtagtctgt acctgcctgt accaagatca cacttgttgt
      181 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
      241 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
      301 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
      361 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
      421 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
      481 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
      541 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
      601 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
      661 tcataatcgt ttcttcttgt gcatttaaaa gaacggttaa aaactttcgg aaaaaaagac
      721 gagtatctac ttttgtcata attttactgt tagcaggttt ctgccaaaat tcataacgat
      781 caaccaatgt ttgtccacga cttattcctt gagtttccac agcagtgaag cttttgaaac
      841 cttcacagat ggccccctcg ataaaataag caacagctaa cggatcattg atgacacatc
      901 ctaaaattct ttcttgtttc cagtggaagt caaaataaaa acgagtgatc gcttttagat
      961 actctccctc ttcaggattc atttgacagc agtattcgag gatagtcggt gtcaagacga
     1021 tttctcttgt cacatctaac ccaaccattt ctatcgtttg tttgagattc tcgaatacat
     1081 aagaagctgc ttcaggatca caccagtaat tgtattcagc aactggcgaa cagttaccat
     1141 ggcttttgta agttccaccc atcgagacaa aacgatccat atgtttccca atatttgggt
     1201 tgactttcaa agcaaaagcg atattggtca aaggacccaa agcaatgact gatgtatttg
     1261 tcttttcttt gaaataatca gccaaaaaat caacggcatg gagaggttct gcctgttttc
     1321 tcaagatcat cgggaaattt gtttccccta aaccatccat accatgagtg tcttgtgcac
     1381 tgatgaaagg ttgcttcaaa ggtttttcca ttccttgata aacaggaatg tctaaacgat
     1441 cacagcgttc caggcatttc agcacattct gcgtacctat atctgcaggg acattcccac
     1501 aaactacggt aatagcaact acttcgatat caggggattt gacagctaat gataaagcta
     1561 aagtgtcgtc gatccctgga tcgcaatcaa taattacttt tcgcattatt tccaatctcc
     1621 tttctttaga gatacttagt tttttattct gggaagtttc cgaaccagtc caagacgagc
     1681 gtcttgatta taatgcacga aattagtata ctggctttta ttgcgaatgt aaatgctctc
     1741 ttacaagtaa atctattgag gagaacttta agtaaatgat acaatgaaaa agaaatggaa
     1801 tttacgtgtg ccgaaaataa agaatcctat ggatcggtac attggataat agttagatta
     1861 tagtttggca gaaagcggcg ggtgtacaat ggttattttt tatttcattc ttattctgat
     1921 aattataggg atcagttacc attttctttc ttatttttat gcaaagaaat tagtaagagc
     1981 gggagtacag agaggatatg actggtatga agacactggt catcgtttga tggaccctgc
     2041 tgcagtcacg tcagtagaaa aaaaacgaca aaaattagaa gaagagtgtg aaagcttttg
     2101 ggagcaggga acacccaaaa caatcaaaag ttatgacggg ttgaaactag ctggtcagat
     2161 gttcatccat ccaaataagc agaataaatg ggtcatttgc gtacatgatt accgaagcac
     2221 agggaaaaga gacatgtctc atataggtaa aagatatgca gagaaaggat tcaacgttct
     2281 tgtacctgat ttacgtgcgc acggagaaag tgaaggagaa atcatcggaa tgggatggct
     2341 agatcggcta gatttaattg cctggatcca actcatctta gatgaacagc cgaatgcgtc
     2401 cattatttta cacggcggat caatgggagc ttctacgatc atgatggcaa gtggtgaaaa
     2461 acttccatca gcggtcaaag gatttatttt agacagcgga tatgtttctg tttatgcaga
     2521 attccgatat atgttaagta aaatcactgt ctttcctaaa aaaatggtca tgagatatgc
     2581 gaatcattat gcccagaaat acgccggtta ctccttaaag caagcatcag ctacgagaca
     2641 gcttggcagc aaccatctgc ctttattggt cattcatggt gaaagagatc attttgtccc
     2701 tacagaagca gcgtacacga ttcaaaatgc tacggcaggg gataaagcac tgctgcttgt
     2761 tccagaagca gaacacttgg aagcatcgat gaaagatccg aaaacctatt ggacagtgat
     2821 tttttcattt attgaacaaa gaataacgat gaattaattt tctcttcttt tttgtaagta
     2881 attcgaggat aaataattgt ttatagatca ggagactgct aaaataaggt aggaataaaa
     2941 aaatatcagt tatttgagga gtgcggcata tggaaatcaa agaagaaaaa aatcgtcttg
     3001 ctttattcaa tgatgaacaa caagagatcg gagaaatgac atggtcagat gcgggcccag
     3061 acatcatgat cattgaccat acatttgtag atccagcata tcgtggtcaa aagttagctg
     3121 aaaagctagt gtatactgga gtagaattag caagacgaga cgggaaaaag atcattcctt
     3181 tatgccctta tgcaaaaaaa gagtttgaaa agaaaccaga atatcaagac gtcttacgtc
     3241 agtcataaga ataacagctg aaagttttga actttcagct gttattccat ttatatagag
     3301 ttgtcacgga cttttatcta tgataaagtt aaagagacga tatcttttgg gaaggaggct
     3361 gcgatatgta taatttaatt ttgggaatcg tgatcgtttt atcgattgtt atggtcattg
     3421 cgattatgat gcagccaagc aagcaaaata gtgcagcgag tgcatttacc ggcggtgcgg
     3481 atcaactatt cggcaaacaa aaagctcgcg gctttgaagc tgtcatgcag cgatcaactg
     3541 cagttatggg agctatctgg atgatcttat tatttgtcct agcatattta tcttcgaagt
     3601 agttgaataa gcctctcgca aaacggcggg cggctttttt tattgcaaag agaacgctta
     3661 agaagaagtt atgaaagaaa ctatggtaaa atgaggcgaa agaggtgaga cgagtgagac
     3721 aattaccaaa acccctttat gcaaaacatg gaaagcgagc ggttttgctg cttcacgctt
     3781 actcaggaag tccaaacgat gttcgaatgc tcgcacgatt tttggaaaaa gcagattata
     3841 cagtttatgc gccgcttttt aaaggacacg gaacaatgga gccatatgat attttgcaag
     3901 aaaaagcaga aagctggtgg gcagacacta aaaaagccat ccatttttta cagtctgagc
     3961 agttttctga tattgccgta ttaggattat caatgggcgg catctttgct gtacgagcac
     4021 tagaagaaga atcagtgatt ggcggcggtt ttttctgttc gcctttgtct cctgtaaaaa
     4081 caaacgtgcc tgaaaacttt gaaaaatacg tacgtcaagt gctgaagaca gcaggtaaat
     4141 cagagaaaga aatcaatgaa aaagcagttg cttaccgctc gttagctgaa caacaattaa
     4201 tggatataca agatcaggca gcaatcgtag aaagtaggtt gtcagatatc caacagccga
     4261 tatttttagc tcaagctggg aaagatgaaa tgatcgaccc taatggcgta tttgaaactg
     4321 cacgcaagtt gagccgtcag cgagtgacac ttcgatggta tcctgaaagt ggtcatgtga
     4381 ttacagttgg tacagcaaga cgcgaattag aaaaagacgt cttggaattt ttagaaaaat
     4441 taccttggaa tgaggaataa atatgacaaa agaaacgata aaagagaaaa ttctcttttt
     4501 catggaaagc agtcgcaaac gcagtttttc tatggaagaa atcgctgaag gtttagggtt
     4561 ccaaaaaagc gatgatttta aattactagt acaaacgatt gctcaaatgg aaagagaaca
     4621 gagtatagtt ttcaataaaa aaggaaaagt gaaacttcct ttaaaaccaa tcttgatcga
     4681 aggaacattt cgagcaaatg aacgaggatt tggatttgta acgattgatc ccgaagaaga
     4741 tgacatctat atcccaaaag aagcgactgg ttttgcaatg gatggagaca ccgtagcaat
     4801 cgatatcatg aagcgagcaa atacagatat ggatcgcgga gcagagggga aagtagtcga
     4861 gattcgagac cgggcgacta ctcagttagc aggggaattc gttgcttatt cagaagaaga
     4921 aatacaagaa acggatcttt acggcgttgt tatcccgaaa gatagaaagc tcaaccaatt
     4981 taaagtatat gcagcagcag agggagttcg tccagttgac ggaagtattg tgctgatcga
     5041 actgacccat taccctgaaa aagggtatgc gactagttta gaagggataa tcaagcaagt
     5101 gattggtcat aagaatgacc ctggcatgga tatcctctca attgtcgtgg ctaatggtat
     5161 ccctacgaaa tttcctgatg atgtattgtc tgaagcagat gaagttccag atcaaatcag
     5221 cgaaaaggat ataactggac gtagagattt aagaaatcag ctcattgtta cgattgatgg
     5281 agaagatgca aaagatctag atgatgcagt tacggttaaa aaattagata acggtaatta
     5341 ttttcttgga gtacacattg cagatgtttc ttactatgtg acacaaggca gtcaattgga
     5401 cagagaagca tatgaaagag ggacaagtgt atatttgact gatcgagtga ttccgatgat
     5461 cccgcaacgt ttatcgaatg gtatctgttc actgaatccc catgttccaa ggctgaccat
     5521 gagttgcgaa atggagattg atccaaatgg acaagtagtc tctcatgata ttttcccaag
     5581 tgtgatccaa acaacagaac ggatgaccta cacagctgtc aatcaaatct tagaagatca
     5641 agacgaagaa acgatgcaac gctatgctca gctcgtacct atgttccaag aaatgggtga
     5701 attgcatcga atccttgaaa cgatgcgaat caaacgtggc gcgatttctt ttgaggatcg
     5761 cgaagcaaaa atccttgttg atgctgaagg acacccaaat gatatccagc taagaagcag
     5821 aggaattggt gaacgcttga tcgaatcttt catgcttgcg gccaatgaaa cggtagctga
     5881 acattataat aaattaaatc tgccgtttat ttaccgaatc catgaacagc caaaagaaga
     5941 aaaaatgcag cgcttctttg attttgcttc agctttgggc attctggtcc gaggaacgaa
     6001 aggaacgatc acaccaaaag atttacagca tgtgatcgaa ggagtagaag acaaaccaga
     6061 agctgcagtt atcaatacta tgttgctaag aagcatgcaa caagcacgct attcagaaga
     6121 caattttggg cattatggct tagcggcaga ttactacaca cactttacat ctcctattcg
     6181 acgctatccc gatctaatcg tccatcgtct gatccgttct tacagtgaag ataaaagtga
     6241 aagcaatcaa agttcgtggg ctacggaact tcctgaaatt gcagatcata gttctaagat
     6301 ggaacgtcgt gcagtagaaa cagaacgtga agtagattcc atgaagaaag cagaattcat
     6361 gatggacaaa gtgggggaag aattcgatgg gatcatcagc tctgttgtga aattcggcct
     6421 gtttatcgaa ctgccgaata cagtagaggg attgatccat atcaatgaat tgaaacagga
     6481 ctacttccat tttgttgaaa atcatttagc tcttgtaggt gaaagaactg gtctgacttt
     6541 taaaatcgga cagaaagtgc gagtaaaagt agtcaaagct gatccagatg aacgagcagt
     6601 agattttgaa cttgtttccg cagaagaggt tacagcgttg gaacggccaa aaacacgaaa
     6661 tggaaaatca ggaaaacgcg aaggaaaaca agcaggaaaa cgcccagaaa gaaatggcaa
     6721 gaagaacaaa cgatatagcg atagtaagca aacgttcggt aagaaaaaag ggaagaagcc
     6781 attttacaaa ggtgttaaaa agaagaaaaa gaaaaaataa aatgagttgg aggaaacagc
     6841 tatgccaaaa ggcgagggaa aattaattgc acaaaacaag aaagctcgcc atgattattc
     6901 gatcatcgac acgatggaag cagggatggt cttgcaagga accgagatca agtcgatacg
     6961 aaacagccgg atcaatctaa aagatggatt tattcgcgtc cgcaacggag aagctttctt
     7021 gcataatgtt catatcagtc cttatgaaca aggaaatatt tttaatcatg atccgttgcg
     7081 cacgagaaag ttattattac acaaaaaaca aatcatccgg cttgaaaatg aattgaaaaa
     7141 tactggaatc actgttgttc ctttaaaagt ctatattcgt aacggctatg ccaaggtatt
     7201 gattggtctg gcgaaaggga aaaaatctta tgataaacgg gaagatttga aacgaaaaga
     7261 tatcgatcga caaattgatc gaacattaaa aaatttctct agataatttc attatccttt
     7321 ttcgcatatt cggtgctttt gcttttactt aatgataaaa tttgttagca tttaatctga
     7381 taaaacatga ttatcatagg ttttttctta gatcaggagc accgtgtata aagaaaaatt
     7441 cgcttcgttt ttgactgaaa taaaaaagtg tctaattttc atgaaaatgt tttgaatttt
     7501 ctgtttcatg gtggtatgat accgtgtggt tagaaaccga ttggaaaaaa ccagatacgc
     7561 acatgaattg attttatgtg aatttcgggg aaaagaggtg aaatggattg gatgaacgct
     7621 cgctaacgtt ccacattgga ccaatttggt ttgatggtac agtttgtata atggtgttac
     7681 tgacctgttt gattgttttc ttcttagctt acttttttac aagaaatctt aagttgaaac
     7741 caacaggcaa acaaaatgcg atagaatggg tcattgattt tagtcgagga atcgtaacag
     7801 ataatctgcc tagaaaagaa ttgagcaatt tccatttgtt ggcatttaca ttgtttttat
     7861 ttgtttttgt tgcaaacaac atcggtttag taacaaaaat cgttttacct aacgaaacaa
     7921 cactttggaa aagcccgaca gcagacccgt ttgttacttt aactttggca tttataatga
     7981 tcttacttac tcacttattt ggggtgaaaa agttaggctt caaaggatat ttcgtaaatt
     8041 cgttcttgaa accatatagt tttatgtttc cgatgaaact gattgaagaa tttacaaact
     8101 tattaacact tgctttacgt ctttatggga atatttatgc gggggaagtt ttgctaacct
     8161 tgattgcaaa aatggtaata agcttaggct ggttctcatt gccgttagca attccattag
     8221 agatggtctg gatcgcattc tccttgttta tcggaagcat ccaagcattt gtcttcgtta
     8281 ctttatctat ggtttatatg agccataaga ttgaagtgga agaataatat tcgttttaaa
     8341 aacaaactac tatttatcta atttaggagg aaaacaatta tgaattatat cgcagcagca
     8401 atcgcaatca tgggagcagc aatcggagcc ggttatggta acggtcaagt tatctctaaa
     8461 acaatcgaat caatggctcg ccaacctgaa atgtctggtc aattccgtac aacaatgttt
     8521 atcggggtag ctctagtcga agctgttcct atcttagggg tagttgtcgc cttgattcta
     8581 gtttttggtg tttaattgaa tcagtattaa ggcagaaaca atttggtttg ttactgcttt
     8641 tttacatgaa aactagaagt acttcaaaga aaggaaggct cgcctatgct gaatcaattg
     8701 gcaattgcag aagttggcaa tgcgatgtta ggaaatgtca tcgttgtcag cggctcattt
     8761 ttgatactac tggctctctt gaagcatttt gcttggggac caatcagcga tattttgaaa
     8821 aaacgtgaag acaagatcgc caatgatttg gattctgcag aacaatctcg catcaactca
     8881 gcgaaaatgg aacaagaacg cgaacaacaa ttgttagcct ctcgttctga tgcagctgat
     8941 atcatcaaaa atgcgaaaga aagtggagaa ttaagccgcc aaaatatttt gaaggatgct
     9001 caagaagaag cagctcgtct aaaaagtaaa gcccaagctg atatcactgt agaacgtgat
     9061 tcagcgctga actctgtaaa agacgatgtt gcagaactat ctcttcaaat cgcggaaaaa
     9121 atcttgaaca aagaattatc tccagaaatg cacgaatcat taatcaatca atatatcgaa
     9181 ggtctaggtt cttcaaatga aactagataa atatacagta ggaagacgtt acgggaaagc
     9241 tttatttgaa ctggcgatcg attcaaataa agcagaagaa atttatcaag atctcgtaag
     9301 cttacgtcaa atctatgaac aagtacctgg attaggtaat atgttaagtg atgttcgtct
     9361 agaacctcat gaaaaacgag aaatcatgga caagcttgtt agtggctatg atggaatcgt
     9421 taaaaacttt ttagaagttg tttatgcgta taaccgaatg gatgatcttt tgttcatgat
     9481 cgaagaatat gaacgtcgat atgatgaaca tcaaggtttg ctgttgggaa gtgtcacaac
     9541 agcagttcca ctttcagacg aacaacgaat acaattagaa aagaatgttg caaaaacaat
     9601 gaactatcaa tcagtcgaat taaaacaaat cgttgattct tctattattg gtggcgcgat
     9661 tgttgaagct aaccatcgag taatcgacgg aagcattcgc acgcaattag aaaatatgcg
     9721 taatcaatta aatagatagt acaaagaggt aggtgaatcg aatggccatc aaagcagaag
     9781 aaattagtgc cttgattaaa gaacaaattg aaaattatca aaacgtgctt tcagtcgaag
     9841 aaatcggtac cgtaacttat gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg
     9901 cgatgagtgg tgagttgctt gaattttcaa atggctcata cggaatggcg caaaacttag
     9961 aatcaaatga tgtaggtatc attatccttg gcgatttcga gtccattcgt gaaggagata
    10021 aagtaaaacg aacaggaaag atcatggaag ttccagttgg agaggccttg attggacggg
    10081 tagttaatcc gctaggtcag ccaattgacg gactaggtga aatcgttact gacaaagctc
    10141 gtccagtaga agcaatggca ccaggcgtta tgcaacgtaa atctgttaac gaaccaatgc
    10201 aaacaggtct aaaagcaatc gacgctcttg taccaatcgg acgcggccaa cgtgaattag
    10261 ttatcggtga ccgtaaaaca gggaaaactt ccattgcgat cgatacgatc atcaaccaaa
    10321 aaggtcaaga tatgatctgt atctatgtag caatcggaca aaaagattct acagttcgta
    10381 cacaagttga aacattgaaa aaatatggcg caatggatta cacaatcgtt gtgaatgccg
    10441 gtgcgtctca accagcgccg ttgctttata tcgcaccata tgctggtact gcaatgggtg
    10501 aagagttcat gtacaacggg aaacatgtct tgatcatttt tgatgatctt tcaaaacaag
    10561 ctgttgctta tcgtgaactt tccttgctgc ttcgccgtcc accaggtcgt gaagcttatc
    10621 caggggatgt tttctactta cactcacgct tattggaacg tgcagcaaaa ttaagtgatg
    10681 atttaggtgg cggttcaatg actgccttac catttgttga aacacaagct ggagatatct
    10741 ctgcttatat cccaacaaac gttatttcga tcactgatgg acaaatcttc ttagaaagcg
    10801 acttgttcta cgcaggtact cgtccagcgg ttgatgccgg actatctgta tctcgtgtag
    10861 ggggttctgc gcaaatcaaa gcaatgaaaa aagttgctgg gactttacgt ctagacttag
    10921 caagttaccg tgaattagaa gcatttacac aattcggttc tgacttggat gcagcaacac
    10981 aagcaaaact aaatcgtgga cgccgtacag tagaaatctt gaaacaaaaa ttgcatgcac
    11041 cgctagctgt tgaaaaacaa gtagtgattt tgtatgcttt gacacatgga ttcttagata
    11101 gtatccctgt agacagtatt ttagacttcg aacatgaatt attcgaatat ctggatacca
    11161 atcattcaga tatctttgaa acaatccgta caacaaaaga tcttccagaa gaagaagcgt
    11221 tgaacagtgc gattcaggaa tataaagaca tgttcttagc tacaaaagga aataattcct
    11281 ctacagaaga taaattgaaa tccattcaaa atgcatagtg aggtgagatg aatgggtgct
    11341 tcattaaatg aaatcaagac acgtatcgct tcaacgaaaa aaacgagcca aatcacgcgg
    11401 gcaatgcaga tggtgtctgc ttctaagttg acaaaatctg aagcttcttc acagaagttt
    11461 caggtttatg caacaaaagt aagagaaatc gttacgcatt tgactgctac tcagttaaat
    11521 gatattgcct cagaaaatcc tagaggggat atcaattaca acagcatgtt gctcagccgt
    11581 cctgttaaga aaacgggtta tatcgtcatc actgcagatg gcggtttagt cggtggatat
    11641 aacagctcga tattgaaaca aacgatgtct attttagaag aagaccacca atcacccgat
    11701 gattacgtga tgatcgcaat tggcggaact ggtgcagact tctttaaagc acgcggtatc
    11761 aatctagcat atgagctgcg caatctatct gatcagccaa gctttgatga agtccgcaaa
    11821 atcgtaaata tggcaacaac tatgtaccaa aacgaagtat tcgatgaatt atacgtatgt
    11881 tataaccatc atatcaattc gctaactagt cagtttcgtg tagaaaaaat gctgccaatc
    11941 tcagacttag atccagaaga agcaactact ttcgaacaag aatatatttt tgaaccttca
    12001 aaagaagaaa tcttgtctca gttattgcct cagtatgctg aaagtttgat ctacggtgcg
    12061 atcgttgacg ccaaaacagc agaacatgct gctggtatga cagcaatgaa aacagcaact
    12121 gataatgctg ctaatatcat tgatgagctg acggtttctt acaaccgtgc aagacaaggg
    12181 gctattaccc aagaaattac cgaaattgtt gccggagctt cggcactaga atagtattga
    12241 gattggagga aaaaagatga gttcaggcaa gattgttcaa gtaatcggtc ccgttgtcga
    12301 cgtggaattt tctttagacc aatccttacc agatatcaac aacgcattag ttgtttataa
    12361 aaatgacgaa aataaatcaa aagttgttct tgaagcagcg ttagaattag gggacggagt
    12421 gatccggacc attgcgatgg aatcaacaga tggtttacaa agaggaatgg aagtcattga
    12481 tacaggcaaa gcaatctctg ttcctgtagg taaagaaaca ttaggtcgcg tattcaacgt
    12541 actaggagat acgatcgatt tagaaacacc tttcccagaa gatgcggaaa gaagcgaaat
    12601 tcataaaaaa gcaccagcct ttgacgaatt aagtacaagt acagaaattt tggaaacagg
    12661 gatcaaagtt atcgatttgc ttgccccata tttaaaaggt gggaaagtcg gactattcgg
    12721 tggtgccggt gttggtaaaa ccgtactgat ccaagaactg atccataata tcgcccaaga
    12781 acatggtggt atttctgtat ttaccggtgt aggtgaacgt actcgtgaag gtaatgactt
    12841 gtattatgaa atgaaagatt caggagttat cgaaaaaaca gccatggtgt tcggacaaat
    12901 gaacgaacca ccaggtgcac gtatgcgtgt tgctttgact ggtttgacga ttgcggaata
    12961 tttccgtgat gtagaaggtc aagatgtact gttgtttatc gacaacattt tccgtttcac
    13021 tcaagctgga tctgaagtat cagccttgtt aggacggatg ccttctgcgg ttggttatca
    13081 accaacattg gcaacagaaa tgggtcaatt gcaagaacgt atcacatcta cgaaaaaagg
    13141 ttcgatcaca tcgatccaag caatctatgt gccagccgat gactacactg acccggcgcc
    13201 ggcaacagca ttcgcccatt tggatgcgac aaccaacttg gaacgtaaat taacagaaca
    13261 agggatttat ccagcggtag atccattggc ttcttcttca agtgctttag cacctgaaat
    13321 cgtgggagaa gaacattata aagtagcaac agaagtccaa cacgtattgc aacgatatcg
    13381 tgaattacaa gatatcattg ctatcttagg tatggatgaa ttatccgatc aagaaaaagt
    13441 gttagtttct cgtgcccgca gagtccaatt cttcttgtca caaaacttta acgtggctga
    13501 acaatttact ggtcttccag gttcatatgt ccctgtagaa gaaacagtga aaggattccg
    13561 cgaaatcttg gaaggcaaat acgatgatct tcctgaagaa gctttccgaa gtgttggccg
    13621 aatcgaagac gtcgtagaaa aagcgaaaac actaggctac tagaaagggg tgccccatgg
    13681 atcaatattt aacagtaaat gtggtaactc ctgccggttt ggtctatgat caccatgcag
    13741 ctatcgtcgt tgcacgtacg acagctggtg agcttgggat tttgcctaaa catgcaccaa
    13801 tcatcgttcc attgacgatt gatgaagttc gtgtaaaacg aacagattca gatacccacg
    13861 tcgattggat cgctgttaat ggcggaatca tggaagttcg tgataatgtt gtttcaatcg
    13921 ttgctgatag tgcggaacgt gaacgtgata tcgatgtatc tcgtgcggaa cgtgcaaaac
    13981 aacgcgcaga acgccaaatc gaagaagcaa aagcaaaaca agatacgaac gaattgaaac
    14041 gggcaactgt tgcgttgcac cgagcaatca atcgtatcaa agtatctaag cactcataaa
    14101 atgagatgcg gatgtttatc cgtgtttaag aacagtaaaa agaggttatg acagaagtgg
    14161 tcagcttcaa gacataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaatct
    14221 cagcttattt ttcgaagaag ctacttctgg aacaccgtat atcggaggtt gtgaaatcag
    14281 cgttttgtcc tgagtattag agaaacaaat ggaaaatagt tcctcatatt ttgccgtttt
    14341 ttgagctaat accgagggtc agatgattga acaccgttta tttggaataa gggactgcga
    14401 caagac
//
LOCUS       DACEJR010000070        14002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEJR010000070 DACEJR010000000
VERSION     DACEJR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.070"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..319)
                     /locus_tag="IW651_002258"
     CDS             complement(26..319)
                     /locus_tag="IW651_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0814973.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="IW651_002259"
     CDS             complement(347..1012)
                     /locus_tag="IW651_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0814974.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="IW651_002260"
     CDS             complement(1152..1784)
                     /locus_tag="IW651_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0814975.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /locus_tag="IW651_002261"
     CDS             complement(1791..2858)
                     /locus_tag="IW651_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0814976.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /locus_tag="IW651_002262"
     CDS             complement(2855..3586)
                     /locus_tag="IW651_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0814977.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IW651_002263"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IW651_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0814978.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIVQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IW651_002264"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IW651_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0814979.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="IW651_002265"
     CDS             5709..6869
                     /locus_tag="IW651_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0814980.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="IW651_002266"
     CDS             complement(6909..7856)
                     /locus_tag="IW651_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0814981.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7989..9380)
                     /locus_tag="IW651_002267"
     CDS             complement(7989..9380)
                     /locus_tag="IW651_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ0814982.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            9506..9916
                     /gene="tagD"
                     /locus_tag="IW651_002268"
     CDS             9506..9916
                     /gene="tagD"
                     /locus_tag="IW651_002268"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ0814983.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            10096..11385
                     /locus_tag="IW651_002269"
                     /pseudo
     CDS             10096..11385
                     /locus_tag="IW651_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11452..12879)
                     /locus_tag="IW651_002270"
     CDS             complement(11452..12879)
                     /locus_tag="IW651_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0814984.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(12876..>14002)
                     /locus_tag="IW651_002271"
     CDS             complement(12876..>14002)
                     /locus_tag="IW651_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ0814985.1"
                     /translation="FFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVISEKIHE
                     KKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRK
                     KQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKKYVSAFN
                     IVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVD
                     NLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEI
                     DVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDV
                     FKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
BASE COUNT         4581 a         2680 c         2074 g         4667 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc acgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcactccaa ttatgcattc tcatacttaa taaagttagt ttaccatttt tatattgtaa
     7921 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt atatacctaa
     7981 aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat agaacccaga
     8041 tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt gataaataat
     8101 gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc ttttactaga
     8161 tttggttgtg tgaaacaata acgcaaagtt ttaaagcatt ggataaataa gaaggttaac
     8221 aatgtcaata gacctaatcc tcctgtagca gccataacta agaaaataaa attatgagca
     8281 gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcgta ttcaatatag
     8341 tatcctggag aaccgccgga tatcggctta ctcttgaata tttcaattgc acttttccaa
     8401 agtccaaacc ttaaattaga aacatccccg ccatcagtgt cagggcgttt taaatcaact
     8461 ttattgctgc tagttgttcc tgatgaatca gatatatctt tttttatttc tgtaatatgc
     8521 ggaacatgtg cccatgcttg agcagttata tctgttacat aatacatcgc aattatactt
     8581 agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt aagaaaacga
     8641 ctactataac ttgtaaagaa actaaaaaca aaagttgcta ttaataaaca aactaatgtg
     8701 gtgcgtgagc ccgataaagc aataaacgag aattgaataa aaatattaat tgcaaaaaat
     8761 attttaaata gaagatttaa atgcttgtaa gtgaccaaat aatatatagc aataatgatg
     8821 acaattaatg atataaccgc accaaaattt ggatctgtat atacaccaaa taaacggttg
     8881 cctaaccacc ctaaacgaac tgggtaatac ttatatttta ttggaatttt tatgccatat
     8941 tgaatgacaa accagtataa tgatactaaa ttagctaaaa accataccaa tgaaagtatc
     9001 caagtaaata ctttgaaaaa aaagttacta ctgtagtctt tgccaaactg ataaatcata
     9061 aagaaaaaga ttaattgcca catgattaac ttgatatttt cgctatatcc atattttaaa
     9121 ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca atcatatttt
     9181 ttaagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac aactcccgct
     9241 attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa atacgggcct
     9301 attgctcgta acattagaat aaaaccaaaa taaataaaat accaaccttt cacatatttt
     9361 aaaaattttt cattcaacat ttttcctcca tccacctatt tctcataaac atttcttatc
     9421 ctttttagga taaactggtt ctttcaattc taacaaaaaa atgctaaact aacaatgtat
     9481 attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta cctttgattt
     9541 gcttcactac gggcatatca atttactgaa acgagcaaaa caatatggag attatcttat
     9601 tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat gttattttag
     9661 ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag ttattcctga
     9721 agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt atttcgtgat
     9781 gggcgatgat tgggctggac aatttgatga acttgaacaa ctaacagatg cacaagtaat
     9841 ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac ttaattcttt
     9901 aaatagtgat aaataatatt ttttaaacaa tgaaagaaca atatatctgg ataagacgta
     9961 aaaaaatggc tctataattt ctaggactga tagaattttc tgtcgatcct agattttctt
    10021 gtaaaaaaaa cagacataca aaaatttcga taaaatagag tcgtctaaaa catcaatctg
    10081 aaaggaagat tttgtatatc catttctcgt tctatcaaag agcacttcac tattaaagat
    10141 aaaaatattg aaattaattc ttttcaaaaa actttttata aggacaagaa agccatcgta
    10201 atcgaaggta cattagttcg cacttttaga cattgtctat tttgtaaaga aactgcgaag
    10261 cagctcgtga aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa
    10321 taaacctatt tgcgtcttag aaaacaacat taccactacc aatcatgcgg aaaatatttt
    10381 actgctcata cgtatcttgt cgttccttca tgttttattt cgaaacaagt tcattacacg
    10441 atattagaag agttgaccga gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt
    10501 tcagtaacga catttcaacg gacattatct gctctatcgg caatgctgcg acagatgcta
    10561 gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat ttcccatgga
    10621 aagttctcct tttcttgtat gaatagagag acagggaagc tctttgacat tttgtccagc
    10681 cggagaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg
    10741 gttcgataca ttgtcacaaa tatgaatgcc ccttattttt cattagtcaa agagtccttt
    10801 ccaaacgccc aaatcatcat tgattgcttt cacattggac aacacttgaa caagtttttc
    10861 gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aagacacttc acaagctaag
    10921 tactatcgac aattgaaacc tttgtataaa ttacttctaa aatcagatga cgagttggat
    10981 tacacaacat tcaagaaatg gcagaatttt caatggcgct atttaacaga aagtgaagta
    11041 gttgatcgtc taccctcaat ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc
    11101 tgcttagcaa ttatcacaag aaacagctag ctacttcgag aaacgaccaa agaagttccc
    11161 tcgaaacttc actatctcaa aaagcctttt tcatatctga aaaaggcttt taactagcat
    11221 tttcgaaaaa gtattctaat aacaaaatta gagaatcttc gcacacatat caaaacattg
    11281 aaacgatttg cttatgggtt tcgcagtttt accaatatgc gcttgtggat ttttctaatc
    11341 aacaggttaa tcagctatgc attaaaagct agtaactctt actaactttg ttgttcataa
    11401 atgaccttat cagcccgatt tgacaaagaa ctactttttt acaacattat tttattttcg
    11461 agtaatcttt cttatttcat ttcgtatatc tggaaaaaga aaataaacaa acggaagata
    11521 aataatgaaa atcattattg atttaattgc catatctaaa aatgaagaag agaataattg
    11581 tggaagaaag taataggccc aagtagtcaa agtaagattg actataaaaa ataagataac
    11641 gttcttccca aagaaatagg atatttttat ttcttttcgt acaaaaaata ctctaaagaa
    11701 aaagactaat aattctacta ccacgagtac gatcgtagcc gctacagcgc cataagtagg
    11761 cacaaaagct acatttccaa taatacttac aatcgcacca atgatcattg gataagcata
    11821 ctccttatct ttttctatag caattgcaaa ttggttagca aaaactccac caattggact
    11881 aacaactata attagacaag aaataagcat gtttattgtc atcggtttga aattctcccc
    11941 aaaaaaccat ggaacaaatt cttttgtatt gataaacacc acactaaaaa gaattaatgc
    12001 aatgataatc gtgtaatcaa aagatttttt tgtgtaaact aaaatatttt ctgatttccc
    12061 ctctgcctgt gctttagata ttttaggcaa cataacaata gtaattgatg ttaagactgc
    12121 caatataata cgagcaattt tttgagactg atcataataa gataattccg ttggatccac
    12181 aaaataccca actatcgttt tgtctaaagt tgtgtacact tgcataaaca actgaggagt
    12241 caaaataata aaagctggtt tccacaactg tttaaaaaca ttagagttaa agattggttt
    12301 acctactttt ccgtggatac ttaaccagaa aactaaactt cctaaaaagt tagtaattga
    12361 agtaatgaac acgtaaatcc acaaatcatc tttagttgaa acaaaaatga ttattaacaa
    12421 taccgatgat aatttgataa ctgtatttct aataaccact ttcgaaaatt cttcaattcc
    12481 ttgaaaaaac caagaaatat ctaatgttag ccctacaata aatatcaatt gaattaagta
    12541 taaattttta tactttacat caaaaaaata aatataggct atatagagaa aggaaacact
    12601 caagcctatt aaaaattgaa taaagtaaat attccagaat tgtaaagata gtgctttttt
    12661 atctttcctt gatttagcga tatttcgaat acgaaaagga ttcattccca cagcaatgac
    12721 agtcattaaa aactgtgata ttgagaaaac atacccataa atccctaaac cagttgaacc
    12781 aattctacga ctaagtatag gtatagtaag taagggtaaa acaatcaaaa agacttgata
    12841 tatcgcatta taaaatatat cttttactgt ttttttcact ttatatccct ctctatcaac
    12901 tcaagaacct tttctgtact tgctcctgtt tcataatttc caatataatc aagtaactct
    12961 tgagaacttt tcaaaagctt ttcttgttgg tttttaaaaa catcaacaag gccataacta
    13021 tcttgtacgc ttataattgg taactcactt aactgtatat aaaaacctct ttctaagcta
    13081 tattcttttt catcatagtt aaaaagaata attggttttt ttgttagcgc aaaatcgaaa
    13141 aaaatagatg aataatctgt gattaaaaca tctatctctt caaaaagcat ttcaagagga
    13201 atcccatcat taattaaagt aatttgatgt tttttcatat attctatact tttagaatca
    13261 gttgctaatt tactatgaac attaggatga aatttagtca taaaatttaa ttcttgttgt
    13321 tgctttttaa aaaattcacg taagttatcc acacctaacc attctagttg cgtaattccg
    13381 ttgtcctcaa tattatttcg aaaagttgga gcaaataaga cattgatttg tttctttttc
    13441 aagtcaaaca cttgctcttt actagaggct aattcatcgt ttctaggagt tcctacttct
    13501 aatattttag aaggaacgat attgaatgct gaaacatact tcttagaact atagcgatta
    13561 ccagataata aatatgtaat tgactcacta tcttttttcg catagcttag gtaacgttta
    13621 ggtaacttat cttttgcatc aaattcaatt ttttttaaag gcgttccgtg ccatgtttga
    13681 aaataaagtt gttttttcct ttttgcatac ttaatcgttt tccgactatc gtctatccaa
    13741 tacttagctg tatataaata aaaggcagct aaaaaagaat cggagtcaat tcttttaata
    13801 taatctggta aatcagttat aatcgcttct tttttgatta accaaataat tctgtaattc
    13861 aatttttttt catgaatttt ctctgaaata actcttggat ttccagaata gttacgcccg
    13921 ccaaaacttg aaaatacaat taaatttttt tgaataggta atattttaat aaaaaccggg
    13981 aaaatattcc ttgaaaaaaa tt
//
LOCUS       DACEJR010000071        13836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEJR010000071 DACEJR010000000
VERSION     DACEJR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.071"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..1043)
                     /locus_tag="IW651_002272"
     CDS             complement(24..1043)
                     /locus_tag="IW651_002272"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0814986.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="IW651_002273"
     CDS             complement(1676..2680)
                     /locus_tag="IW651_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0814987.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="IW651_002274"
     CDS             complement(2693..3742)
                     /locus_tag="IW651_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0814988.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="IW651_002275"
     CDS             complement(3759..4580)
                     /locus_tag="IW651_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0814989.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="IW651_002276"
     CDS             complement(4567..5346)
                     /locus_tag="IW651_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0814990.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="IW651_002277"
     CDS             complement(5363..5833)
                     /locus_tag="IW651_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0814991.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="IW651_002278"
     CDS             complement(5845..6264)
                     /locus_tag="IW651_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0814992.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9113)
                     /locus_tag="IW651_002279"
     CDS             complement(6345..9113)
                     /locus_tag="IW651_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0814993.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            9313..9537
                     /locus_tag="IW651_002280"
     CDS             9313..9537
                     /locus_tag="IW651_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0814994.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10316..12031
                     /locus_tag="IW651_002281"
     CDS             10316..12031
                     /locus_tag="IW651_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814995.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12034..13797
                     /locus_tag="IW651_002282"
     CDS             12034..13797
                     /locus_tag="IW651_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0814996.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4065 a         2831 c         2402 g         4538 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaataaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
    10141 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
    10201 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
    10261 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
    10321 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
    10381 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
    10441 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
    10501 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttcaca
    10561 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
    10621 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
    10681 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
    10741 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
    10801 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
    10861 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
    10921 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
    10981 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
    11041 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
    11101 aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata tgaatcaaat
    11161 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
    11221 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
    11281 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
    11341 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
    11401 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
    11461 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
    11521 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
    11581 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
    11641 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
    11701 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
    11761 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
    11821 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
    11881 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
    11941 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
    12001 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
    12061 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
    12121 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
    12181 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
    12241 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
    12301 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
    12361 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
    12421 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
    12481 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
    12541 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
    12601 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
    12661 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
    12721 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
    12781 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
    12841 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
    12901 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
    12961 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
    13021 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
    13081 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
    13141 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
    13201 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
    13261 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
    13321 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
    13381 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
    13441 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
    13501 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
    13561 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
    13621 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
    13681 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
    13741 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaaaa
    13801 caagcagtct tctgggatgg gaagactgct tgtttt
//
LOCUS       DACEJR010000072        13537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEJR010000072 DACEJR010000000
VERSION     DACEJR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.073"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(60..1007)
                     /locus_tag="IW651_002283"
     CDS             complement(60..1007)
                     /locus_tag="IW651_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0814997.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="IW651_002284"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="IW651_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0814998.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="IW651_002285"
     CDS             complement(1642..2004)
                     /locus_tag="IW651_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0814999.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="IW651_002286"
     CDS             complement(2015..3136)
                     /locus_tag="IW651_002286"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0815000.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="IW651_002287"
     CDS             complement(3152..3502)
                     /locus_tag="IW651_002287"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0815001.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="IW651_002288"
     CDS             complement(3632..5143)
                     /locus_tag="IW651_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0815002.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="IW651_002289"
     CDS             complement(5465..6817)
                     /locus_tag="IW651_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0815003.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="IW651_002290"
     CDS             complement(6970..8046)
                     /locus_tag="IW651_002290"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0815004.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="IW651_002291"
     CDS             8242..9987
                     /locus_tag="IW651_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0815005.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="IW651_002292"
     CDS             complement(10236..10703)
                     /locus_tag="IW651_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0815006.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="IW651_002293"
     CDS             complement(10687..12060)
                     /locus_tag="IW651_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815007.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="IW651_002294"
     CDS             complement(12381..12869)
                     /locus_tag="IW651_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0815008.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13493)
                     /locus_tag="IW651_002295"
     CDS             complement(12891..13493)
                     /locus_tag="IW651_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0815009.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2939 c         2457 g         4278 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttggcacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    13441 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    13501 ccccacatct tttttcattt attaaaactg cctcctt
//
LOCUS       DACEJR010000073        13147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEJR010000073 DACEJR010000000
VERSION     DACEJR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.074"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..149)
                     /locus_tag="IW651_002296"
     tRNA            complement(77..149)
                     /locus_tag="IW651_002296"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IW651_002297"
     CDS             530..1099
                     /locus_tag="IW651_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0815010.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IW651_002298"
     CDS             1086..1946
                     /locus_tag="IW651_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0815011.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IW651_002299"
     CDS             2136..2840
                     /locus_tag="IW651_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0815012.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IW651_002300"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IW651_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0815013.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IW651_002301"
     CDS             4677..5993
                     /locus_tag="IW651_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815014.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IW651_002302"
     CDS             5994..6863
                     /locus_tag="IW651_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815015.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IW651_002303"
     CDS             6918..7727
                     /locus_tag="IW651_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0815016.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IW651_002304"
     CDS             complement(7830..8459)
                     /locus_tag="IW651_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ0815017.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IW651_002305"
     CDS             8629..9921
                     /locus_tag="IW651_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0815018.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IW651_002306"
     CDS             complement(9997..10659)
                     /locus_tag="IW651_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0815019.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IW651_002307"
     CDS             10884..11168
                     /locus_tag="IW651_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0815020.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IW651_002308"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IW651_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0815021.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACEJR010000074        13140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEJR010000074 DACEJR010000000
VERSION     DACEJR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.075"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2122
                     /locus_tag="IW651_002309"
     CDS             <1..2122
                     /locus_tag="IW651_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815022.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="IW651_002310"
     CDS             2119..2739
                     /locus_tag="IW651_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0815023.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            3432..3812
                     /locus_tag="IW651_002311"
     CDS             3432..3812
                     /locus_tag="IW651_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0815024.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="IW651_002312"
     CDS             3794..4708
                     /locus_tag="IW651_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0815025.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="IW651_002313"
     CDS             4948..5637
                     /locus_tag="IW651_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815026.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5757..5843)
                     /locus_tag="IW651_002314"
     CDS             complement(5757..5843)
                     /locus_tag="IW651_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0815027.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            6489..7421
                     /locus_tag="IW651_002315"
     CDS             6489..7421
                     /locus_tag="IW651_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0815028.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            7438..7908
                     /locus_tag="IW651_002316"
     CDS             7438..7908
                     /locus_tag="IW651_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0815029.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7992..8309
                     /locus_tag="IW651_002317"
     CDS             7992..8309
                     /locus_tag="IW651_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815030.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            8599..9780
                     /locus_tag="IW651_002318"
     CDS             8599..9780
                     /locus_tag="IW651_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0815031.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            complement(10009..11157)
                     /gene="tnpB"
                     /locus_tag="IW651_002319"
     CDS             complement(10009..11157)
                     /gene="tnpB"
                     /locus_tag="IW651_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0815032.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(11174..11578)
                     /locus_tag="IW651_002320"
     CDS             complement(11174..11578)
                     /locus_tag="IW651_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0815033.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            complement(12114..12575)
                     /locus_tag="IW651_002321"
     CDS             complement(12114..12575)
                     /locus_tag="IW651_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815034.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            12708..13082
                     /locus_tag="IW651_002322"
     CDS             12708..13082
                     /locus_tag="IW651_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ0815035.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         4828 a         2036 c         1994 g         4282 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg tgaaagatac gaaagtggaa gaagcttgta gtgatttatt tggagataca
     5461 attccttttt caactagcga acaagaacga aaagaacgac aacaatcagt cacatatgaa
     5521 cgctatgaaa tcttgcttca acaagcaatt aacatacaaa atctgactaa aaaagaaaca
     5581 gctttaaatt atttaaaaag cgtcaaggaa tggttagatc agcaacagca aaattgaatt
     5641 tgattgaaat tcatgttaaa ctaaacatgc aaatgttcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttaggttgat gtcgccttta ctgattttac
     5761 ttcttgtttc ggttattcaa ccaataactg aatacagaga tacaaactcc tacaagaagg
     5821 gggcaaataa acagttcaaa catcagatac acctccttcc agtcgcaaga ctgtcggata
     5881 ggcgacaagt tattataccc tacaaaatta aatctgtgtg gcaattttaa gtatctctta
     5941 tttccgggtt gttagggctt gccctgaccg cctgtagggc gctttaaatt ccagataaag
     6001 ttttttatac taagactgtc ttaaaacggc ttagacaagc tcttagagcc tcaaccctcc
     6061 tttttgaaca atggttttag caaaggagct ggaggcgact gcgcaggtat aatgtgagag
     6121 cgtagcgatc acaccttttt ggcgaccgta gggagcgagg ttttgaatta gcagtttaga
     6181 aaagttcgcg aacctgttta aagaaaaaat aattgttttt ttaggtggga aattttgtct
     6241 ccctctttta taatcttttt ttaaacctct ttttaaacct ctttaacgtc ggctgtgtcc
     6301 ttactccccc aaggtataca aaattttata ggcatgaaat tatctgttta taggcatgaa
     6361 attatctgtt tataggcatg aaattatctg tttataggca tgaaattatc tgttttaggt
     6421 attgttttta gacataatat gtcctaaaat gatgtcaata aaatcattta gactggagta
     6481 acaactaaat ggcaaatgaa atcgtaaagt atgaaccaga attaaatacc attcctttaa
     6541 gaaaatttac cccaatagaa atgaacttgt ttttttctat tgtctctaga atgcgcgata
     6601 aaggtaatca aaaaatacgc ttttatttca gccaacttaa agatttaagt gcgtataagc
     6661 agactgcagg agaagtcttt ataaaagatt tggcaagaac atacgatcat ttgatggatt
     6721 tacgtttcgg aagaaaaagc aatggtggat taacaataga acgatttaca atgtttactg
     6781 attttgaaat tcatggagaa gtcgataacc cttatgtaga tataaaagtc cacgaaaaag
     6841 ctttgccttt acttaataat ctagaaagct gggtacgttt ctctttaaca gaatttcgag
     6901 atttaaatag cagttatgct aaaacaatgt ttagattgct aaaacaatac cgaactcaag
     6961 gatgggctga attttctaag gaagattttt tagagctatt agatattcct aaaagctata
     7021 gacaaacaaa tataaatcag tttgtactca aaccaataaa agaagaacta actccactat
     7081 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttattggtt
     7141 atcgttttac ttggaaagct gaaatcaacc atgctgacga tttttcaaaa gggaagcaag
     7201 aagatttacg tataaaatta tttaatattg aacacaatgg agaattaaca caaaaagaaa
     7261 aatggcgtgc taaagatcga atactaaatc ttccgttagg tactcatgaa gctgatttta
     7321 aaaaacaaca acaaaccgaa aaagaagaag cggaaaaaca atcaatctca aatgagctta
     7381 aacaagattt attagaaaac ttacaaaact tattcgattg aagcataagg agtatttatg
     7441 atagataaaa cgatcaaaga actcgcggaa gaatttcatg tttcaaaaca agcaattagg
     7501 aaaaggctat cagctgattt tagagaaaac tatgtgcaaa ctgttactag gaacggagtg
     7561 aaaaccttag ttgtcagcta caaaggatat ttgcttttaa aacaacattt tttaggtagt
     7621 aacaccactg gtaaccaagt agaaaccgtt gctagtaacg atgaaaactc gattattgtt
     7681 tttttacatg aacagctcca agaaaaagat actcaaataa aacaaatgca aaagttactc
     7741 gaccagcaac aacaactcac cttgcaggca aatcaacaga tcgaaaaact gcaagaacag
     7801 ttacaattag cttatacgga agaacctgaa gaagaggaat taacttctgt caataaagaa
     7861 aacaacaatg caataacaac agaaaaaaag tggtggaaat tttggtaaca attaccaact
     7921 ttccataaag aagaaatcaa tttctataga ctctatcttt ctaagaataa aaaatatgtt
     7981 taggtgatca aatgactata agtaaagtaa tcaaacaaaa aagaatagaa aaacaactaa
     8041 ctcaagaaga tttagctgaa atgcttttag tatctaaaaa aaccatttct aattgggaaa
     8101 atggtcgaac aataccagat acagaaaatt taattcagtt tgctaagtta tttgatttat
     8161 cactagacaa tattttgtta ggagaagaaa aaattatggt aaaaaattat ggtaaaaaat
     8221 catacaaaaa cactaatact attatttcat tttgtaattt ttttaagttt agttttcacc
     8281 ttaattcagt ccggattctt ttattttagc cattcaattg ccacttggtt attaatattt
     8341 tttctaagtt tactattaat aattaataca ttaataatta atactttaat tattatcttt
     8401 aataaacata gaaacttatt tcttcacagc atatttccaa ttgttttatt aatttttcta
     8461 tactttatat aaaaagatag aactatttag taagataaaa aagtagctaa ttgtaatttt
     8521 cgtctaaaac tcaaaactag taaaaataac tttattgtgt tatttttatt tataaattat
     8581 gaaaaaggac ctttcgaaat gaaaaataat agaatctaca aaaatcagtt gaattatggg
     8641 cttttattgc cagtttttct gctatgtatc attggattac tttctttata tgttgcttta
     8701 tatcacgact cttacactaa ttcaaaaatc acaaaagaat taattaaaca agtattgtgg
     8761 tatagtattg ggttcacttc agcaataact atttattttt ttaatcctaa aattttgtgg
     8821 aaattaactc ctatatttta cggattatgc atcataatgt tattacttct cttgaaattc
     8881 tataatccac aattagcagt cttaactgga gcaaaaaatt ggttttatat tgctggtttt
     8941 tcgtttcaac ctactgagtt agtcaaaata ggatacaccc ttatgctagc taaaataacc
     9001 gtcaattatc agcaaaaatc tattactagt aatacgaaag atgattggat ttatttactt
     9061 aaaactttta ttgctactct tccaatagga atattattat tttttcagaa cgatctagga
     9121 acaatgttag tcttcattgt tatttatatt tttattttgt ttacgtctaa cataaatata
     9181 aaaataattg ccccaataat cgccattggt gttgttttat ccttaatatt tttatatttg
     9241 gttatatcag atacaggaag agatttctta ttaaaaatag gatttcacga ctatcagttt
     9301 aaaagaatag actcatggtt gaatccattt ttagatccaa atggatctag ctatcagtta
     9361 gcaaatagtt taattgctat tggctctggc ggacttttag gtactggttt taatgtatca
     9421 aacattcacg ttcctgttag agaatccgat atgatatttt ctgtaattgg agaaaatttt
     9481 ggctttatag gaagttgtct tgtaatattc ctatactttt atttaattta tcaaatgtat
     9541 aaaacatgtt ttaaaagcaa taacttattt ttcacctata tcggtatagg cattgttgca
     9601 atgatttttt ttcatgtttt tgaaaatata ggtgcaagta tcggattact acctctaact
     9661 ggtattccac tcccctatat tagtcaaggt ggttctgcca ttcttagtaa ttttttgggg
     9721 ttgggattta tgttgtgttg tagtagatat actaacgaaa aaaaaggaac acaaaaatga
     9781 attcaaaata acactttatg aatagaatgt ctatttcaat aaaaattatg ataccttaaa
     9841 gttaatcagt gaactactca ccacttagct aaacctgacg gttcttaacg cttgaagtgg
     9901 gagcttcttg ggaatagagc atacttgata gcgtatttct ttactaacag cggtgtctct
     9961 tatttaccaa gctatccccg tagttcctac ggttcttgat gattggatct aagccaatcc
    10021 catagaatgt agtcttagac cttcggtcag aatattgata ctagcattga tatcacgatc
    10081 atgatgagta tgacaaacag gacaagtcca ttctcgaatt tcaagagatt tcttgccatc
    10141 tttatgtccg cactctgaac aaatctgact agacggaaac catttatcta ctttgatgat
    10201 ttttcgtcca taccagtcag ccttgtattg taatttagtc acaaactttg accaagaaac
    10261 atcagaaata cttttagcta atttatgatt tcgcaacata ccttttgtgt ttaagtcttc
    10321 aatacagata atatcgtgat ttttgatcat ttctgtactc aacttattca gaaaatcagt
    10381 acgttgattc attacttttt catgcaatct agctactttg cgtttttgtt tttgataatt
    10441 tttagcttca aatagattgg tacctttatt tttagccaat aaagcacgcc tagacaattt
    10501 gcgttgttca cgctttagtt tcttttccat tttggacgtg aatttattat tatcaatttt
    10561 ttgtccatca gaaagaatcg caaagtccat aatacctaag tcaataccaa ttgtggaatt
    10621 agttttaggt aattcgataa tttcttcttt acacaacaaa gaaatatagt atttaccgct
    10681 agaatgacgt gatattgtag cggatttgat aatccctttt ggttgtctat gaagcttaat
    10741 tcttactaat gacttcaatt taggaacttt gatgaattta ctatcaatca aagcaactgt
    10801 accattttga ttattcgttg tataactctg aacaggattt ttcttacttt tgaaacgtgg
    10861 aaatccaacg gatttatccc gaaaaaaatt cttgtatgct ttatctaaat taagttgggc
    10921 attggctaaa gcaaggctat caatttcttt caaaaacgga aattctttct tgtatttagc
    10981 tggtgtcgga aaagtcattt ttttagaaga atcattttta acttcttcat acgccttttt
    11041 tcggtcatca agcattagat tgtagacctt acggacacaa ccaaaagatt tggcaaagaa
    11101 gatttcttgt tcctctgttg gatagattct gaatttgtat gcttttagtt gttccataaa
    11161 gttcacctac tttctattta tgattgtttc cttgattttg gatatacttc ttaatcacat
    11221 caattggtgc gccaccagtt gtcagaagac aaaaactttt agaccagaac atttctttcc
    11281 aaagaaattg tttgactctt ggaaagtcac gttttatcaa tcttgagctg gcacttttat
    11341 aggcattgat gaattttgtc aattctgttt taggttgagc tttgaacata atatgaacgt
    11401 ggtctttatc atgattccac tccactaaag taatgtgata cgattctgaa attctttcaa
    11461 aagtagtttt ggcataatca gatatttcat catcaatcac ttgtctacga tatttcgtta
    11521 ctaaaacaag gtggtaataa agaagaaata ctgaatgatt atttgtatct aattccatgt
    11581 tatatcaact catttcttgt atagactgat tgtaacatgg aacaaaataa aaaggcaata
    11641 cgccttagtt gtcggtcttc gagttaccaa cggtaaccct cataccgaac ggcattcatc
    11701 acccacctat agaggatggg cgacttctgc ctaattctgt taaattcaaa ttattacaac
    11761 agtcctaacg aagaattgtt tgtaaaacaa acggataaat aataaaaaag actgctcttt
    11821 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttatgaagc
    11881 gctaccaaaa atttaccata ttttacaaaa aataacaagt cttaaaaaaa tgacagataa
    11941 aaatgcacaa agaattagat atagataacc gcgaaaaatc aagccttaaa aagagctttt
    12001 gacctagacc aatgaaaaat cacaaagtga gcccctgtat cccttggtag agaggctact
    12061 tggcatagtc gacattatgt caactatgca attaaataac taactacttc atattagctt
    12121 aataaaataa tcaaaataac tagggggaaa aacattgtat aaaaaaattt ttcatttttt
    12181 aaataagaat agtaaatata aaaataattt aagatatcta ttactattaa tccagataga
    12241 gcaaaccaag taaatattcc atgtgtaaag ctaaaaaaat tcatttgaat caattcaaat
    12301 aaacctaata tagtaacaaa tatattaaaa ataatagctg tcaataccag taaattattt
    12361 tttttatttt ttgaattatc ttcatttaaa atagaattta tgttagtact ataaaattca
    12421 caaatttttt ttagtgtctc tatatctggt gttgttttgt tattttccca ctttgaaata
    12481 gtttgacgtg taacgttaaa ttttcttgcc acatattctt gagtataccc ttttttcact
    12541 cttaattctc ttagcttaga tcctaaatcc tccatttttt ctcctatgta aacgattatt
    12601 tacataaaat tttatcataa aaatactaac gcgccaaaaa agttctttat tttataaaac
    12661 acactaaaat aaaataattc tagtaaaaaa gataggagaa ataagttatg aaaaaatttt
    12721 tatgtctttc tgctttaatg ggtgtcttat taactagtgg aggaatagct agtgccacag
    12781 aagctttaaa tctagatgta cccgaagacc ataccgccat ttacggagga ggaatggaag
    12841 ctacagaaga aggattttct tctagtaaat tgagatatgc tgctggcggc ggggatttca
    12901 actgtggggt taatggattt aaagtttatg caaattacta ccatgctaga gctaaacata
    12961 gtgcaacagc taaaaatgga cgaggcggtc aagtacgatc agttcagaaa gctggagtaa
    13021 gagcttatgc aacatgcaat gccacactta ctggaaatac tggttggtgg aatgtttatt
    13081 aaaaaagaga gtttaaactc tcttttttat ttacatttga aaggaaattc aaaatatgtt
//
LOCUS       DACEJR010000075        13039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEJR010000075 DACEJR010000000
VERSION     DACEJR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.076"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..1716)
                     /locus_tag="IW651_002323"
     CDS             complement(136..1716)
                     /locus_tag="IW651_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0815036.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="IW651_002324"
     CDS             complement(2117..3493)
                     /locus_tag="IW651_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0815037.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="IW651_002325"
     CDS             complement(3621..4739)
                     /locus_tag="IW651_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0815038.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="IW651_002326"
     CDS             4881..5315
                     /locus_tag="IW651_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0815039.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="IW651_002327"
     CDS             complement(5393..5926)
                     /locus_tag="IW651_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0815040.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IW651_002328"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IW651_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0815041.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IW651_002329"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IW651_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0815042.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="IW651_002330"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="IW651_002330"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0815043.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="IW651_002331"
     CDS             complement(9651..10229)
                     /locus_tag="IW651_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0815044.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="IW651_002332"
     CDS             10551..11021
                     /locus_tag="IW651_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0815045.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="IW651_002333"
     CDS             complement(11129..12355)
                     /locus_tag="IW651_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0815046.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3865 a         2629 c         2192 g         4353 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagcgcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaa
//
LOCUS       DACEJR010000076        12782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEJR010000076 DACEJR010000000
VERSION     DACEJR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.077"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            156..1343
                     /locus_tag="IW651_002334"
     CDS             156..1343
                     /locus_tag="IW651_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ0815047.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1324..2628
                     /locus_tag="IW651_002335"
     CDS             1324..2628
                     /locus_tag="IW651_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0815048.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2654..3667
                     /gene="citC"
                     /locus_tag="IW651_002336"
     CDS             2654..3667
                     /gene="citC"
                     /locus_tag="IW651_002336"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ0815049.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3671..3964
                     /gene="citD"
                     /locus_tag="IW651_002337"
     CDS             3671..3964
                     /gene="citD"
                     /locus_tag="IW651_002337"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ0815050.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3969..4874
                     /gene="citE"
                     /locus_tag="IW651_002338"
     CDS             3969..4874
                     /gene="citE"
                     /locus_tag="IW651_002338"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ0815051.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4867..6426
                     /gene="citF"
                     /locus_tag="IW651_002339"
     CDS             4867..6426
                     /gene="citF"
                     /locus_tag="IW651_002339"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ0815052.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6423..6980
                     /gene="citX"
                     /locus_tag="IW651_002340"
     CDS             6423..6980
                     /gene="citX"
                     /locus_tag="IW651_002340"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ0815053.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6973..7836
                     /gene="citG"
                     /locus_tag="IW651_002341"
     CDS             6973..7836
                     /gene="citG"
                     /locus_tag="IW651_002341"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ0815054.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            8070..8479
                     /gene="tnpA"
                     /locus_tag="IW651_002342"
                     /pseudo
     CDS             8070..8479
                     /gene="tnpA"
                     /locus_tag="IW651_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9116..10288)
                     /locus_tag="IW651_002343"
     CDS             complement(9116..10288)
                     /locus_tag="IW651_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0815055.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
     gene            10962..11774
                     /locus_tag="IW651_002344"
     CDS             10962..11774
                     /locus_tag="IW651_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815056.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT         4371 a         1960 c         2440 g         4011 t
ORIGIN      
        1 ttttctcatt ataccaaaaa aattgaattt agtctttttt taaattttta aaaataagca
       61 tcattttttt aaaatttaaa atgaaagggt ttttttattt taaaaaaagt gttaaagtgg
      121 ttttgtaatt aaaaaatgta aaaaggagtg aaggtatgct tgatgaagtt ttagaaatcc
      181 ataaaaaaaa tgtaggtgtt ttacggatgg aaacaacaga agccgtgcaa gatgatcaag
      241 atttagcaaa aatgtacacc ccaggtgtag cagaactaag tttaatgatt gagaaaaatc
      301 atgctttggc aagagaatta acaattagtg gtaaattagt ggccgttatt acagacgggt
      361 cggcggtatt aggtcttgga aatgtaggaa accaagctgg attaccaatc gtcgaaggga
      421 aagcgctgct ttataaaaat ttagcaggtg tcaatgcgat tccattagcc atcgaacaaa
      481 aatcagtaga cgagattgta caaacaattg tcaatttgca aaatagtttt gcagggatcc
      541 atttagaaga tattgcagca ccgaagtgtt ttgagattga agaaaaatta caagaaaaac
      601 tttctattcc tgtgtatcat gacgatcaag aaggaacagc gatagttgtt ttagctggat
      661 tgatcaatgc tgctaagatc caaaggaaaa cattaactga acttcgagtt ttgatcaacg
      721 gcatgggagc atctggggtt gcaacagcta gactattaat cgctgctggt atcaaaaatc
      781 taacattggt ggataaacaa gggagattaa agaagcaaga tagtacatta aataaatatc
      841 aaagagagtt gttggatcat atacatgata caaataataa tgcaagcgtt ttggaagatg
      901 caatcgttgg acaagatgtt tttatcggtc tttcaatagg aaatgtttta acgcctgaaa
      961 tgattggaaa aatgaatgat aatccgatta tttttgcttt agccaatcca gtaccagaga
     1021 tcacacccga tttagcaaaa gaatctggtg caaagctaat cgctacaggt tcttcgaaat
     1081 atcctaacca agtaaacaat attttagtat ttcccggatt gttcaaggga ctattactat
     1141 cgaaagcgaa taaagtcgat ctttcattac aacttgaagt agccaaacaa ttagctgcta
     1201 aggtcgcaga gcctacggta gaaaaattca tccctaatgt ttttgatgaa ggagtcgcaa
     1261 acgtcgtttc acaggcagtt ttggattatt tacaaaaact aaaagaaaac gaggataata
     1321 tacatgctga aaacattaaa tagaattaaa attggtttta ttcctttatc tctttatatc
     1381 attttggtac tcgcgtatct tgcaatgacg ataagcaata tcgttccaga aaatgatatg
     1441 ttagtttctt tgggtattat gactgttttt gcttatttat tagaagaaat tggtaaaact
     1501 attcctattt taaaagattt aggtggtaaa gtattagttg ttacattttt gcctgcattt
     1561 ctagtctata aacattggct acctcaaagt actgtaactg tggtttctga ttttatgaat
     1621 aataacaatt tcttgatgtt ctttatcaca ctactagttg tcggcagtat tgcttcaatg
     1681 aatcggcaaa cattggttaa agcaacttca cgtattatca ttattttaat tttatgcgat
     1741 gtgattggtt ctttgatagg aacaggtgtg ggaatactcc ttgggctatc tgcttggaat
     1801 gcgtatttct ttatcgttgc gccgattatg gctggtggtg ttggggaagg agcgatgcca
     1861 ttatcgattg gttatgcagc aatattatct ggtatgggac aaaatgatat ctttgggaag
     1921 attctaccgt gtgtgatgtt gggaagttta gtcgcagttc tactgtctgg tatgctcaaa
     1981 aaaattggag agaaataccc acaattaaat ggtcagggta atttgatcga tggggatgat
     2041 gaacatttag agaacttgaa gggttcagct aaaccagtca atattgaaaa aacattgtta
     2101 gcaggcgtgg tagcaataac gatttatttt gttgctgtct atgttgagca cttattaaaa
     2161 tcactccttg gcttttctat tccttcacct gtgatcttat tagtctctct tatgggggct
     2221 aaaatgcttg gttttatccc taaaaatatc gaagaaggtg gaaattcgtt gtatggattc
     2281 actgttcgtg ggatcacacc tcccttactt tttggcgtag gggttgcaaa aacagattgg
     2341 ttagagctga tcgaagtatt taaaaatcct gcaacgatag taacaatcat tgtgacggta
     2401 gtagctattg tcgctacagc ttttatcgcc gcaaaatttt taaaaatgta cccagttgat
     2461 actgccatcg cagtttcatg ttgtagcgga caaggcggta caggtgcttt agctatctta
     2521 gctgcgggtg atcgcatgga attaatgcca tttgcacaag ttgctgttcg actagggggt
     2581 gctatgaccg taacgttcgc tatcttttta atgggtctgc tatcataaga acataaagaa
     2641 aggacgtgac catgtgtttg atcaaacaga tatccaacga ttatttttaa aaaataacta
     2701 tatcaaagac caatgggaaa atcttttgct tgattcagga atacaaaaga agcaaatcga
     2761 agattttcaa catttagatc aaacattggg tatataccat aaagaaaagt tagtaggaac
     2821 agtatcttat caaaacaacg tgattaaata tatcgccgtg tctgaaaaat ataaagatgg
     2881 tggacataca tttaatgtac tagtctctgc aataattcag gcaatgagtc aacaagagat
     2941 ttttcattat attgttttca cgaaaccgcg ctatcaaaaa agttttaact ttttaggttt
     3001 tgaaacaatc gtatctaccc caaaaatagc tgtattagaa atgggagata gttctataaa
     3061 acagtaccta gagaggattc ctaaagtgaa tggtgaaaaa aaagcagcaa ttgtcatgaa
     3121 tgcgaaccca tttacagaag ggcattatta tttagtaaaa caagctgcca ataacaacga
     3181 ttgggtatat gtatttgtac tcgaaaaaga acaaaatctt ttttcgacga aggaccgact
     3241 caaattagtt caacaaggat tacaaaaact gaaaaatgtc attgttctat caggtggcga
     3301 gtatattatt agccctgcaa cgtttcccac ctatttttta cgagaagatg atgaaatagc
     3361 aaaggagcaa atggtcgtag atgctacttt attcaaagaa agaattgcca cttatttaga
     3421 tatctctgct agatatgtgg gagaagaacc actttcccca atgactcgtt catataacga
     3481 catactggaa acgattttac cccctgaaat aaaagttcat gtccttgcaa ggaagaaaac
     3541 ggaacaacac caagttattt ctgcttctca agttcgtaaa gcgtatcttg ctgggcaatt
     3601 ggaaaaaata aaatatatgg ttccagaaac aacctatcaa tacttaaaaa ataaaaggga
     3661 gcgttaagta atggaaataa ataaatcagc gattgctgga acattagagt caagtgatgt
     3721 ccaaatcatg atttcaccga atacagaagg aatagaaatc gatttagaca gtcaagtgat
     3781 tgagcaattt ggaaaacaaa tcaaaaaagt tatctatgac acactagcag tttatcagat
     3841 taaaaacgta aaactcaagg tagtagataa aggggcatta gattgcgtta ttgttgcacg
     3901 attaaaaaca gcgatccatc gttccttagg tatccaacac caagaaacac cgtgggaggt
     3961 ttaattagat gatgaaagaa cgtttgagaa gaacaatgat gtttgttcca ggaaataatc
     4021 cagcgatgat caaagatgca ggtatttatg gtgctgactc gattatgttc gacctggaag
     4081 atgcagtgtc tatggcagaa aaagacagcg caagaatgct tgtggctgag gcattgaaaa
     4141 cagtggatta tggtgacaca gaattaattg ttcgaataaa tgacctgcac tcagaatttg
     4201 gacgtgcaga catttttgca atggttaaag ccggcgcgca tgtattacgt ttacctaaaa
     4261 cagaaacctc tgaagatata acagaagtag aaaaagtgat tatagaagca gaaaactatc
     4321 ataatgttcc gatagggcga acattgatga tggcagccat tgaaggtgct tctggtgtat
     4381 tgaacgcacc tgcgatcgcc aaagcatctg atcgattgat tggaatcgcc attggggcag
     4441 aagactatgt gacaaatatg aaaaccaaac gatatccaga tggaaacgaa ttgttttttg
     4501 ctcgcagtat gatcgttcat gctgcacgtg cagcaggcat ttccgcattt gacactgttt
     4561 actctgatgt ggatgatatg gaaggatttt taaacgaagt acgtttaatc catcaattag
     4621 gttttgatgg aaaatcttgt atcaatccac ggcaaattcc agtagtcaat actgtttatc
     4681 agccgtcaat caaagaaatc aaacgatcac tagacatcat ctcggcaatc gaagaagcaa
     4741 aagaaaaagg ttctggcgta atttctttaa atggaaaaat gatcgataaa ccaattgtag
     4801 aacgtgcaca acggacattg atgttagcaa aagcatcacg attgattgat gaggagggaa
     4861 atatccatgc ataataaaca aagacgtagc attccatcaa gtgttttaac aaagtataaa
     4921 aaagaattcc aaagtgtttc attatcgcca caaacgattc aacgttgtgc accaaaagta
     4981 acgactgtaa ctaatcagac aaataaattg atgcactcga tcaaagaagc tatagaaaaa
     5041 tcgggcttaa aagatggaat gacgatttca tttcatcatc atttgagaga aggtgatttt
     5101 gtttttaacc aagtgatggc cgtgatccgt gaaatgggca tcaaagaatt gaccttagca
     5161 ccaagttctt taacgaacac tatgaatgag atggttattg aatgcatcaa ggacggtaca
     5221 gtaactcata tcacaagcag tgggatgcgc ggcagcttag gtgaatttat ttctcatgga
     5281 ggaatggaca atcctgtgat tttacgttct catggtaatc gtccacgtgc aattgaaagt
     5341 ggtgaattga aaatcgatgt tgcatttcta ggagtaccaa catctgatga ttatggaaat
     5401 gctaatgcga ctgttggaaa atccgttttt ggttcattag ggtatgcaaa agtagatgca
     5461 caatatgcag ataaagtcat tcttctaact gatcatttag ttccttaccc taatacgccc
     5521 attagtattc cgcaaacaca agtcgactat gtcgtggaag tagaagcaat tggtgatccg
     5581 aataaaatcg gtgctggtgc tacacggttt actaaaaacc caaaagaatt aaaaattgct
     5641 gaaatggtca aagatgtcat tgttcactca ccttatttca aagatggctt ttcttttcaa
     5701 acaggaacgg gtggtgcagc tttagcagta acgcgttact tagctagtga aatggaagaa
     5761 aaacagatca aagcttcttt tgcattaggt ggcatcacta aaccaacggt tgatttattg
     5821 gaaaaaggtc ttgttgaaaa agtgttagat gtccaagact ttgacaaagg agcagctgaa
     5881 ggaatggcgc ggcttgagaa ccaacaggag atcgatgctt ccttttatgc tgatccaagt
     5941 aataaaggag cggttgttaa ccaattagat gtcgctattt tatctgcatt agaaattgac
     6001 acaaacttca atgtaaatgt tatgactggt tcagatggtg tgctaagagg tgcgattggt
     6061 ggacatcaag atgcagcaac ggctaaactt actatcatct cagcgccgtt agtacgtggc
     6121 cgaataccta cagtagtaaa tgatgttact acattgatca caccagggaa aagtatcgat
     6181 gtattagtca cagaagtagg ggttgctatc aatccccaac gtaaagatct aatagctatt
     6241 tttgaaagaa ttccacaaat tcctgttttt acaatagaag aattacagca aaaagcagaa
     6301 aaaatagtag gcatcccgga acctttgcaa ttcacagatc ggatagttgc ctatgtagaa
     6361 taccgagatg gttcaatttt agatgtcgtt cgtcaggtga aagaatttga agaagagaga
     6421 tcatgatgtt tgatactgga gaaattcaag aaatcgagga tattttgatc cataaagaac
     6481 aacgtgctaa cttgcaaagc aacctgcttt ccaagtattc aaatagttca ttgatggtct
     6541 ttacattgaa tattcctggg ccgatcaaaa ataatccaat gattgagcag gtatttcgtc
     6601 agggacagca gtcaattcag caaatgtttt taaaaaaaaa ggtggaaatg acgtacacaa
     6661 aagaaatcaa tttgccaagt ggattggact ggtacgtaat agtaacagaa gcacctttga
     6721 atctcaaaaa atggttggtc acgttagaag agcaagacgc attaggtaga ctttttgatt
     6781 tagatgtctt gttcaaaaaa ggagagcaga taacaattgt cagtcgccaa gctcttaatc
     6841 atggggaacg tccttgtttt atttgtggga aatcagcaaa ggtatgtgca cgtaatcgta
     6901 cacatactgt gaaagaaatg caaaagaaaa tcgaacaaat agtagccgat tccttaaaag
     6961 gagcattgaa acatgcatga taatattcaa aaaatagcac aagaagcaca attagcgatg
     7021 ctttatgaag ttacatgtat caataaacca ggattggttg atccagtaga ccccgggtct
     7081 catcaagata tggacatttt tacttttcta caaagtagtg tagttttgac tccctatttt
     7141 gaagaattta tccaaacagg attggaccga caacatcaac caattgaaga aacctttatg
     7201 gcgattcgac agattggttt agaggctgaa actgcgatgt tctcagtgac aaatggtata
     7261 aatacacata aaggtgctat cttttcattg gggatttttc ttgctatttg tggccggtta
     7321 actatttgga aagttccttg taaaaaaagt ttctttcaaa aagaaattca aaaaatgacg
     7381 aaaaatctac ttaacgattt tgggaagatt gatcaaacaa aaccaaaagc atggacttgg
     7441 ggagaatatc tatttgttaa ccatggattg acagggatac gaggggaagc caaaagaggt
     7501 tatccttgtg tttttgaccg aggattgcct tattatcaag catatcaagg gtcgcaacaa
     7561 gataagatga tagacacttt actttttcta tcaatggaag tagaagatac caatttgatc
     7621 aaacgaagta ataatagaca tgtttttgaa gagtatcaat tacttattcg tccttatttt
     7681 gaattagggg gtgtaaaaac catacaaggt aaaaaatatt tggattacct aaatcagcaa
     7741 tttaaaaaga aaaattggag tattggtgga agtgcagact tattgatcct tacaatcttt
     7801 ttagataaaa tcatggacaa aggatggtta tgttgatact ataatacatt attttgtatt
     7861 agtaagctaa taactaaaaa atggttcaat acaaatattt tataaaaaat ggtagtgcct
     7921 atcctttcag attgtagaca aagaaaaatt tctttgtcta caattttttt gttaatgact
     7981 tcagtcagtt tcgtgcgcaa agcgtgcgaa acaagaaaac catgcgtttc tacacgtgga
     8041 agaactagca tatcgaaagg agttttttca tgtcaaatga cgataaaagt ttagcccata
     8101 ctcgttggaa ttttaagtat catattgtat ttacaccaaa atatcgacga aaagtaatct
     8161 ataggcaatt acgaaaagat attggaaaaa tacttaggaa gttgtgcaaa ataaaaaacg
     8221 tggaaattat agaagcacat gcaacgccag atcatattca catgcttgtt caagtaccat
     8281 caaaaataag tatatccagt tttatagtta tctgaaagat cgtagtgctg tgctaatcca
     8341 cgagcagcac gtaaatcaca aatatagata cggaaataaa tcattttggt cggaggaata
     8401 ttatgtgagt acagtcgaat tgaatcaaaa gacaattgaa aaatacatcc gtgaacaaga
     8461 agctgatgat cgcatttgag atagtataag taaaagagag tagatgttgc aataacaaaa
     8521 gggtaaaaat ggactacctc cagtaaaata ccgaaaaata gtttaacttt tggagataag
     8581 tccatttaag ggtctataga atgacaattt ttttagggga ttttactatt attacttcaa
     8641 gacccgcttg gattcgcaaa gtgagtgaac tcttaaaact acctgatagt aggttgtttt
     8701 aagagttatc tgaaagtaaa aattgcattt atatttttga taaatacgta aatttatata
     8761 tacgtattta tcaaagtttt aggccaaagg aatattgtgt aaatacacta ggattgaatg
     8821 aatagaccag aagaaattgc gtccaagaac aaaagaagca tgatagtgct ctatataaat
     8881 tgggcattca agaatctgaa atttaaaggt agaattttag ttagactttt agattttaaa
     8941 cacaaaaata accatacaag caacaagaag caaagtgcta tattataaat tgtccgtccg
     9001 tttacttaca taagatcatt ggggctattt ttaaaagaga ataatagata ataaatgaaa
     9061 aaaaagtcaa gtccacaatc ctgtgtaaaa tactatacaa tgctctcact attttttact
     9121 tggcaaattt aatatatttt cttgatgaag aaagccactc atcgtgttga tcaataagga
     9181 ctgctccaat taaacgattg gctgaaacgc agtttggaaa gatacgaata attttttctc
     9241 ttctgcgcac ttcttgattc agtcgttcaa ttagattggt actctttagt cgattgtggg
     9301 aatttccttg tacggtatat tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag
     9361 cttttgaata ttttggttga tcgatataat catgaatcaa tcgattttta gcctcacgag
     9421 ctaagttaat atctgtgaac ttaaaaattc ctttaacagc ttctctgaaa gattttgaat
     9481 tttttttagg aatggtggta aagatatttc ttaggaagtg aacttggcat ctttgccaac
     9541 ttgcgttggt gaaggatttt ctaatggcag agactaatcc tttgtgcgca tcagaaataa
     9601 cgagttctgt accttgtaaa ccgcgttctt ttaggtattc aaaaaatgtt atccaggtct
     9661 cttcgctttc gccactttga atcatgaagc cgataatttc acggtcgcca tctttggtta
     9721 ttccaatcgc tatatgacag ctttttgaga gtactcgatt ttcttctcgt acttttatat
     9781 agagtacatc ggtcattaag taaggataat ttttttctga taataaacga ttctgccact
     9841 cgttaaccat tggttctagc tgttctgtta agctagaaac gaaggactta gagacggatt
     9901 taccacaaag ttcttccaca atttttgata ctttacgagt tgaaacgcct gatacataca
     9961 tttccaacat tgaagcaatg agggcttttt cgtttcgttg ataacgttca aacactgtgg
    10021 gtgaaaaatg gccatcacgt gttctgggta cttttaattc tagcgtgcct atacgtgtcg
    10081 taaagctgcg ctcataatag ccatttcgtt gactttgtcg gttttctgtt cgttcatatt
    10141 cttttgcttg aatatattct gttcgttgat tttccattag ttgattaaat accgttgtta
    10201 aaatattttt agaaacgtca tcctttacag aatattcaat aatgctttga atctcttcgc
    10261 ttttcagtgt aaaatgtact tgggtcatgt aaaagtcctc ctgggtatgt ttttgtcgtt
    10321 aaaaacattg taccgtaaaa ggactgttac atggcctttt tacttttaca caattataag
    10381 gactttatcg aaaaaaagca gttatttgtt aaaatcattg atattattaa taataagtat
    10441 tttttgaaag gggaaatata aaaggttttt gaaaataaat aaaaaatagt attacttgtt
    10501 ctattgctag ttattgctag tctttattct tttttgtttt ttagggatgt acaaaagatc
    10561 tcaaccacag gagacattgc ttttcacttt tctagaataa aatgcttgtc ctcagtattt
    10621 tctggtccaa taaattttac cacatttaat agctatggcg gaggcgtgaa tttttttatc
    10681 catatctaac atctttccag ccgtaatatt ttactggaca acaaacaatt tgattctaag
    10741 ttatatactt actatacgtt gggatactga atatctgtac aattctagtg agctatcaat
    10801 atggacaaaa gaatagatgc agccttctta ttcagctgtt tgtatagctt ttatggattg
    10861 atatatatta gcgtactgca atttcagaat caattgcgtt gacgattgta ccagttgtct
    10921 tgtattacac atatactctt ctttatgaaa gaaaaaactg cgtgattctt ttagctgtca
    10981 gtatgagttt gctcatctat acccatattc tctccacctt tatgagtgtg attgttattg
    11041 gcttcttgtt tatcggctct cttttctcta agcggaaaga gagaaaagaa atgtcgaatc
    11101 tgatatcaaa tatgataaaa gctgttggtt ggaccattct tttaacagct tacgtttggt
    11161 atccgatgtt acaacaagtc ctacatcaaa aaattaatcg tccttttcgc acaaaacttc
    11221 aagaaagggc attaaacatt tctgatagtt taatcggtgc agtaaataat gatttagtta
    11281 catttacaat gggaacaatt ggtgtggttt catttattct tccacttttc tttaaaaaaa
    11341 aatttgccaa taaagaaaag attattaatt tttgtgcagt gaccacttgg ttcttatcaa
    11401 cgaatctttt tccatgggaa tttttacaaa aaacacctat tcaaatcatt cagtttcctt
    11461 ggagaatctt gggttttcaa gtgctttttg gttcattgat cttaattata gtgtttttaa
    11521 agtggaaaac aagcaacaaa aaaagtatgt gtagccttgt aggaatagtc cttcttattt
    11581 ttacagtgac tgtagcaaca gaagcgaatt attcgcagaa aattcaatcg tataaaggtc
    11641 gtttaattat gaccaaaaaa gatgttacac tttatacaac ctcgagaact ggaggtcttt
    11701 atgactatgc accattagat gcattgaaat ataaagatca tttaaagaaa catgaagtaa
    11761 aagtctatga ttgatgggag aatgcatctc ataaagcaca taattctaaa ataatctata
    11821 cagtaggaga tgcgaatgag caaaatatca cgttacctgt atttaattac tgggaacagt
    11881 tatgaaagta aatgggaaaa agagcactat agaaaatgac aatggtttag tgtctattaa
    11941 aggtacggga aaaagaacta tagttgaggt ttcatctacg tatccaacta cgatgattct
    12001 atctttatta attagttcaa taacttttat attcttattt acacgttatt taataagatg
    12061 ctgcggttaa atgaaaatag attagataga tattataaat atgttgattc tattttcata
    12121 gcaatggaga aaatacatta gcaatatata aaagaaggta tcaagagtca cggtgaattt
    12181 attccgaaaa gtgaaaagaa caaatataaa atttaataaa aatatagaac taaataggaa
    12241 aacgtagacg taattttcta cgttttcctt ttcatatata ttaaatatta cggaaattta
    12301 tataaactaa tttagtgttt tgtttgtaat tcctaatttt gctccacttt tagataattt
    12361 ttaggtctat ttaactcatc agacattcta tgctcatttt ctccaaattt gtttcaactc
    12421 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
    12481 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
    12541 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
    12601 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
    12661 catttattga ttgcaaaatt atgtgacaaa aagaactagt agaagaagga ataatggctt
    12721 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
    12781 ct
//
LOCUS       DACEJR010000077        12739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEJR010000077 DACEJR010000000
VERSION     DACEJR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.078"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW651_002345"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW651_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0815057.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="IW651_002346"
     CDS             complement(321..890)
                     /locus_tag="IW651_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0815058.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="IW651_002347"
     CDS             1076..1978
                     /locus_tag="IW651_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0815059.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW651_002348"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW651_002348"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0815060.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW651_002349"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW651_002349"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0815061.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYKGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW651_002350"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW651_002350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0815062.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="IW651_002351"
     CDS             8546..8731
                     /locus_tag="IW651_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0815063.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="IW651_002352"
     CDS             complement(8793..9989)
                     /locus_tag="IW651_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0815064.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="IW651_002353"
     CDS             complement(10159..11595)
                     /locus_tag="IW651_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0815065.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="IW651_002354"
     CDS             11888..12697
                     /locus_tag="IW651_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0815066.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2672 c         2249 g         4015 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tcctttgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaattttt
//
LOCUS       DACEJR010000078        12661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEJR010000078 DACEJR010000000
VERSION     DACEJR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.079"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1168)
                     /gene="queA"
                     /locus_tag="IW651_002355"
     CDS             complement(137..1168)
                     /gene="queA"
                     /locus_tag="IW651_002355"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0815067.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1361..2536
                     /locus_tag="IW651_002356"
     CDS             1361..2536
                     /locus_tag="IW651_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0815068.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2540..3181
                     /locus_tag="IW651_002357"
     CDS             2540..3181
                     /locus_tag="IW651_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815069.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3178..4095
                     /locus_tag="IW651_002358"
     CDS             3178..4095
                     /locus_tag="IW651_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0815070.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4092..4763
                     /locus_tag="IW651_002359"
     CDS             4092..4763
                     /locus_tag="IW651_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815071.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4959..5793)
                     /locus_tag="IW651_002360"
                     /pseudo
     CDS             complement(4959..5793)
                     /locus_tag="IW651_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5994..6260)
                     /locus_tag="IW651_002361"
     CDS             complement(5994..6260)
                     /locus_tag="IW651_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0815072.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6260..6523)
                     /locus_tag="IW651_002362"
     CDS             complement(6260..6523)
                     /locus_tag="IW651_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0815073.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            6998..7183
                     /locus_tag="IW651_002363"
     CDS             6998..7183
                     /locus_tag="IW651_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0815074.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7325..9148)
                     /locus_tag="IW651_002364"
     CDS             complement(7325..9148)
                     /locus_tag="IW651_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0815075.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9745..12450)
                     /locus_tag="IW651_002365"
     CDS             complement(9745..12450)
                     /locus_tag="IW651_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0815076.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGDTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3649 a         2636 c         2386 g         3990 t
ORIGIN      
        1 cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa catcaaaaag
       61 tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta
      121 gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc
      181 gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca aaggcactaa
      241 ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct tctaccactt
      301 tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct ttgatttctc
      361 cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg acagccacga
      421 ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc gtcaaacggt
      481 agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt cggaatgtac
      541 ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg atctcatcaa
      601 gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca ttttctttcg
      661 catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat ggaggcaatg
      721 gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca aagtcaatga
      781 tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca tcaccaaaag
      841 agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc cacgtatctc
      901 cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt ttttctccat
      961 ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca ggctgtaact
     1021 cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga tccaaaacaa
     1081 gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct tccggcaagt
     1141 caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat gtgcttcatc
     1201 attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc tattttttgt
     1261 tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc atactagtaa
     1321 tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat ttcaaaatgt
     1381 gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa
     1441 gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga cgatgcggat
     1501 gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa aatccatcca
     1561 agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa gtattggatt
     1621 gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt tgaaagtcag
     1681 tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat tgccccgtga
     1741 gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta ttggagtcgt
     1801 tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg gtgcgcttga
     1861 tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc gcttgggaaa
     1921 aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa gccgtatcgc
     1981 catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct tacgtcatcc
     2041 tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag ccaagccaaa
     2101 tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac cggaaaaatc
     2161 attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt tagttaccga
     2221 taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa agcgaggaaa
     2281 agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag aatccgccta
     2341 tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc cggtcgttga
     2401 tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata tcgtctacga
     2461 tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata ctcctgaaga
     2521 atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag aaattctctc
     2581 aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta tcatcattgc
     2641 tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg tgattggctt
     2701 aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga tcccgctatt
     2761 tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat tgccgatttt
     2821 acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg
     2881 catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg ctatgccaac
     2941 gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta cacttgcctc
     3001 ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata attaccaacc
     3061 agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg ctgattggct
     3121 tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga atgaagaatg
     3181 aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc gagttgttct
     3241 ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg tggaatcact
     3301 tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta tacagataaa
     3361 aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc gatgatgaac
     3421 ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac tacattgaac
     3481 cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa
     3541 agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt ctttcttgtt
     3601 cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa aaatgtagcg
     3661 gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga cggttatgac
     3721 ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca gattggactt
     3781 gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc tacggatgga
     3841 cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt cccaccatat
     3901 gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt aaaagaagcc
     3961 ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa ctacgaagca
     4021 gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga aaaccactat
     4081 tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt ttctattatt
     4141 tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg atttctatct
     4201 atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt tctaggagac
     4261 gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc ccttctctag
     4321 ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg attgttactg
     4381 tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg atccaagtag
     4441 acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa cgactattca
     4501 tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac gcgttagttg
     4561 ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt ggagatatca
     4621 ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt gcattgccaa
     4681 ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga cggctagatc
     4741 cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg
     4801 ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca ctctgattct
     4861 tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg tatactccaa
     4921 cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt cttatccgct
     4981 aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc
     5041 taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc
     5101 tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct acatcatcaa
     5161 aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat
     5221 cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg gaggtttgcg
     5281 gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag acttggcggt
     5341 acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata
     5401 agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct
     5461 attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat tcatgttcgg
     5521 caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt atttcgtatc
     5581 tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg
     5641 ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc
     5701 aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa
     5761 cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac
     5821 ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac
     5881 aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct
     5941 cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca
     6001 taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac tttgtaaacc
     6061 actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttc
     6121 tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat
     6181 tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga gtttttgatt
     6241 gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc
     6301 cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa
     6361 gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg tttccactac
     6421 gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa
     6481 aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt
     6541 ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa gcacttaaat
     6601 tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg gaggatcttt
     6661 gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa gtaattggta
     6721 acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg caacaacacc
     6781 aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata acctgaggat
     6841 acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag tcttctactg
     6901 cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg atttgatact
     6961 tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt atggtaacaa
     7021 aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc acttaagagt
     7081 cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca aaggattcgg
     7141 aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa gaataataac
     7201 atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc tgggaataag
     7261 acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga ttttaaaact
     7321 tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca cgccaagaca
     7381 atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag cgtctgtttg
     7441 gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca tttttactta
     7501 cgatatctac ataacggata attggatttt caatcgagcg ataagaagag tggacgattg
     7561 aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt tctttttcga
     7621 catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat
     7681 tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg gtattcccgt
     7741 cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact tttttataat
     7801 gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag aaaggccaaa
     7861 tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg aacgcacctg
     7921 taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat ttgacgacca
     7981 tccctgtttg agataaagca aatggaatga acacgccgat agagtataac ggaatcaagc
     8041 gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct agtgtgataa
     8101 tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc atgaatttat
     8161 cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta gcagcaactg
     8221 ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt ccaaagacag
     8281 ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca taccaatagt
     8341 tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag
     8401 cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta atcgcctcca
     8461 ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac
     8521 ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct gtcacgatat
     8581 tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac accggaaata
     8641 ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt agcaaaacga
     8701 ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat gtgatcgctt
     8761 cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat aaagagccac
     8821 cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat gctcccccgc
     8881 catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc aataaaataa
     8941 taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa gcgactaaaa
     9001 cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac gacaataatg
     9061 ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt aaaggcttac
     9121 ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca attttacttc
     9181 aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca ctgaccttaa
     9241 aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa gcgctttttc
     9301 aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt tgaattctat
     9361 aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat atcttaactt
     9421 ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga agcatagcgc
     9481 agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt aataagccca
     9541 accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact tgatgcagaa
     9601 cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg gcattagctc
     9661 aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa
     9721 acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt
     9781 gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac
     9841 aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt aaatgcctcc
     9901 tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg
     9961 gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac
    10021 taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac
    10081 gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg
    10141 gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc
    10201 cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg
    10261 gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac
    10321 ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata
    10381 aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcatc
    10441 tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc
    10501 gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc
    10561 agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc
    10621 ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa
    10681 tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat tgatgccatt
    10741 gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat
    10801 tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt
    10861 cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg ccccttgcgc
    10921 agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac tgatttgacc
    10981 tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag
    11041 caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg
    11101 gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgcaa
    11161 tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc
    11221 attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt
    11281 ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc
    11341 tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt ttgtgatgct
    11401 tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc
    11461 ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga tgatcgtcaa
    11521 gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc
    11581 gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt cttgcaaatc
    11641 aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt
    11701 ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt tattcagttt
    11761 ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat
    11821 cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga tacttactag
    11881 gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt
    11941 tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt
    12001 cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc
    12061 accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta
    12121 atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc
    12181 taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc
    12241 ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatctcc
    12301 atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat
    12361 cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata
    12421 taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga
    12481 attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt
    12541 ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa ataattaagg
    12601 attacagact ttactttatc atgaaataaa agggaaagat aattttatgc caaagttttt
    12661 a
//
LOCUS       DACEJR010000079        12598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEJR010000079 DACEJR010000000
VERSION     DACEJR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.080"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            556..756
                     /locus_tag="IW651_002366"
     CDS             556..756
                     /locus_tag="IW651_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0815077.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="IW651_002367"
     CDS             1324..1887
                     /locus_tag="IW651_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815078.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="IW651_002368"
     CDS             complement(1948..2292)
                     /locus_tag="IW651_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815079.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="IW651_002369"
     CDS             complement(2389..2634)
                     /locus_tag="IW651_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815080.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="IW651_002370"
     CDS             complement(2704..2958)
                     /locus_tag="IW651_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815081.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="IW651_002371"
     CDS             complement(3122..3445)
                     /locus_tag="IW651_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815082.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="IW651_002372"
     tRNA            3632..3705
                     /locus_tag="IW651_002372"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="IW651_002373"
     CDS             complement(4090..4587)
                     /locus_tag="IW651_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0815083.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="IW651_002374"
     CDS             complement(4627..5436)
                     /locus_tag="IW651_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0815084.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="IW651_002375"
     CDS             5770..6627
                     /locus_tag="IW651_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0815085.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="IW651_002376"
     CDS             6620..7522
                     /locus_tag="IW651_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0815086.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="IW651_002377"
     CDS             7555..7905
                     /locus_tag="IW651_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0815087.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7939..8877
                     /locus_tag="IW651_002378"
     CDS             7939..8877
                     /locus_tag="IW651_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0815088.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="IW651_002379"
     CDS             8955..9848
                     /locus_tag="IW651_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0815089.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="IW651_002380"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="IW651_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0815090.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10463..10651
                     /locus_tag="IW651_002381"
     CDS             10463..10651
                     /locus_tag="IW651_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0815091.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10664..11566
                     /locus_tag="IW651_002382"
     CDS             10664..11566
                     /locus_tag="IW651_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0815092.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11598..11840
                     /locus_tag="IW651_002383"
     CDS             11598..11840
                     /locus_tag="IW651_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0815093.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12096..12467)
                     /locus_tag="IW651_002384"
     CDS             complement(12096..12467)
                     /locus_tag="IW651_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815094.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4461 a         2113 c         2366 g         3658 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
    10501 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
    10561 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
    10621 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
    10681 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
    10741 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
    10801 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
    10861 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
    10921 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
    10981 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
    11041 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
    11101 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
    11161 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
    11221 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
    11281 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
    11341 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
    11401 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
    11461 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
    11521 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
    11581 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
    11641 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
    11701 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
    11761 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
    11821 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
    11881 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
    11941 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
    12001 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
    12061 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
    12121 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
    12181 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
    12241 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
    12301 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
    12361 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
    12421 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
    12481 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
    12541 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatg
//
LOCUS       DACEJR010000080        12271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEJR010000080 DACEJR010000000
VERSION     DACEJR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.081"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            806..1006
                     /locus_tag="IW651_002385"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IW651_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IW651_002386"
     CDS             1493..1672
                     /locus_tag="IW651_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815095.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IW651_002387"
     CDS             complement(1718..2323)
                     /locus_tag="IW651_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815096.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IW651_002388"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IW651_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IW651_002389"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IW651_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IW651_002390"
     CDS             3358..4329
                     /locus_tag="IW651_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0815097.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IW651_002391"
     CDS             4469..5782
                     /locus_tag="IW651_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0815098.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IW651_002392"
     CDS             5825..6691
                     /locus_tag="IW651_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0815099.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IW651_002393"
     CDS             6688..7536
                     /locus_tag="IW651_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0815100.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IW651_002394"
     CDS             7550..9022
                     /locus_tag="IW651_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0815101.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..9911
                     /locus_tag="IW651_002395"
     CDS             9039..9911
                     /locus_tag="IW651_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0815102.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PYPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(10147..11529)
                     /locus_tag="IW651_002396"
                     /pseudo
     CDS             complement(10147..11529)
                     /locus_tag="IW651_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11991..>12271
                     /locus_tag="IW651_002397"
     CDS             11991..>12271
                     /locus_tag="IW651_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815103.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3824 a         2082 c         2465 g         3900 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac ttcttcaaac
     9181 aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc
     9241 gcgattccgc cacttatggg tacattacct cgaccccgaa attggcatgg caaaattttg
     9301 atttcatcgg taccatgaaa aaagaatttc cgatcccgat ttcttggaca acagatgtca
     9361 atgcggctgc ctatggtgag tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt
     9421 actatacgat cggtacgggt gttggcggcg gtgccttaca agaagggcgc tttatcgaag
     9481 ggtttagtca tccagaaatg gggcatatgc tcgtcgtccc ttatccaaaa gatgactttg
     9541 ctggcagctg tccatttcat ggcaactgtt tagaagggat ggccgctgga cccgccatcg
     9601 aaaaacgctt agggaaaaaa ggacaagagg ttgcagaaga tgatccttat tgggaaatcg
     9661 aagcctccta tattgctcaa tgtgcctaca atacaacctt gatgttttct ccagatgtga
     9721 tcatttttgg cggtggtgtg atgaaacaac gccatatgct gcaaaacgtc cacgacgcat
     9781 ttgcaaaatt ggtcaatggg tatgtcgaaa cacctgagtt aagcaaatat atcgtgaccc
     9841 cagcattaga agacaacgct ggaacgctcg gttgtttggc attggctcga gaagcggtct
     9901 tacattcata aacgaaaaaa ggctcttcga catttagtgt tggtagagaa agatacaact
     9961 gaaaatttag tcataaccag ttaggcagag tgcttaactg gtttttgttt tacttggaag
    10021 atcaagcctt gaatgagata agttcttgct cgaaagttat ggaaatttcg atagccgtag
    10081 gctacttgta ttgtgcaata gttttttaca aaaaaaaggg ggtgcggata ttttttttgg
    10141 acctttttaa caaatatatc tttgcttctt ccgatattga agagggctta gccctcctaa
    10201 agataactta attcgttgat tattatacca atgaatataa tcatccaatt tgtttataaa
    10261 ctgattgatt gttgtattct tccagtcacg ttgataaaac atctcgtttt ttaatcgtcc
    10321 aaagaaacct tcgcacgctg aattatctgg tgaataaccc tttttagaca tagagtgaag
    10381 caagttggct ttgttccaat agtccaacta acaatagcac catcgtaaca atcgacaagt
    10441 ggtgataagt agattttccc tgcgagaatt ctaaattcag taatatctgt taaccatttt
    10501 tcatttggct tctttgcttt aaaattccga tttaataaat tagggtgtgc aggagaaatt
    10561 tccccagcat acgaagaata cttttttcgt ctagaaaatt taacaaatag attttcctca
    10621 gtcatcaatc tacgtacaac tttttcagaa agctttttac ccatattttt taaagcttgg
    10681 tgaattctac ggtaaccata cgcacagaag ttatgattga aaatcgtgat aattaatttg
    10741 acgttctttg taatacttat ctggtttctc aagtgcttgt ttttgataaa aatagctgct
    10801 tttaggaaac tggagctgtt gaagaagctc tttcaaggtg aactgatttc gtaaggcatc
    10861 aatcaatatc gttttttctt gatttgatag acaaagaaga ttgacgcctt tttctttttt
    10921 tagcagttca gccgctcctt cgagtaatgc tttttcgagc tgtaattggt gtacttgttt
    10981 ttgtaagtct tctacttcct ttaaaagtag ctctttttgt ttttcctgat tttttaaagt
    11041 catacacaat ggaaaatcct ttccaagaac cgtgtttttc cattgatata gagtaactct
    11101 actaattcct ccagattcgg ctaaattttt cctattagtt tgcggtttat agaatttttt
    11161 caacacagct tctttttgat cttgtgttaa ctttaccgca ctttttcgga gttttctagc
    11221 tcctggtgca agttcttcac accgctgccg caaggactct ctattaggat aaccaagcat
    11281 gcgaatggtt cgtgcataac attgaccata ttcaaaataa tgattcaccg ctattcgttt
    11341 ttgttcttca gaatactttg atttttttcg gtgctcctga tggagctctc cggattccaa
    11401 atactcttta taccatttcc gtagtgcacc aatcgacgga taccctaatt ctcgaatggc
    11461 tgcagcataa gagcaatcgt attttaaaaa taactgaatt gcccttacac gctctttgtg
    11521 ggaaaacaca cggacattcc tcctaagttt attttttcgt ccaggattat gtccgcaccc
    11581 ccttgctttc tgatgactta ttctggttct ttgagtgtca atgcatagat gtgatgtagc
    11641 tagttagaaa aaaattttga atactctttt tttctttcag actccttttt aagtaagatt
    11701 ttgaagtgtg agttacgggg tcagaaactt aaaagttttt gatcctttat tttcaagtct
    11761 gatttggcag cttttcggca gctcatttat ttatggtcag aaatagatgc tgaaatttta
    11821 atctattagc aaatagtcca gaacgtatga agaggagtcg gatatcattt gtcattaaca
    11881 ccataaatat ttataaaatt cgtgttcacc tacaattaaa tttaatatat aatattcgtt
    11941 gtaagggctt ataatttttt gttaatgaac aagataggaa gaaacattta atggacagtt
    12001 ttcagagatt gtcactagta aatcaatttg agttattggc taaatttgag gaccaaaaca
    12061 gatcttatta tgagagaaaa atcgaaatat tgagagaagg ttacgagtat cattatgatg
    12121 atgaaatttg gagtgatctt tctgagccat ttccgaaaga agattctcga tttgtattag
    12181 atgtattgaa catgtatcga gatatcaatt ttagtaaatc aaagctaaac ttagatgaaa
    12241 accaaattac taattcatac tatactcatt t
//
LOCUS       DACEJR010000081        12239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEJR010000081 DACEJR010000000
VERSION     DACEJR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.082"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(248..1201)
                     /gene="menA"
                     /locus_tag="IW651_002398"
     CDS             complement(248..1201)
                     /gene="menA"
                     /locus_tag="IW651_002398"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0815104.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1214..2317)
                     /locus_tag="IW651_002399"
     CDS             complement(1214..2317)
                     /locus_tag="IW651_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0815105.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2407..3342)
                     /locus_tag="IW651_002400"
     CDS             complement(2407..3342)
                     /locus_tag="IW651_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0815106.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3644..5578
                     /locus_tag="IW651_002401"
     CDS             3644..5578
                     /locus_tag="IW651_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0815107.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            6035..6451
                     /locus_tag="IW651_002402"
     CDS             6035..6451
                     /locus_tag="IW651_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0815108.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6465..7031
                     /locus_tag="IW651_002403"
     CDS             6465..7031
                     /locus_tag="IW651_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0815109.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            7054..8040
                     /locus_tag="IW651_002404"
     CDS             7054..8040
                     /locus_tag="IW651_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0815110.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8195..10006)
                     /gene="scm"
                     /locus_tag="IW651_002405"
     CDS             complement(8195..10006)
                     /gene="scm"
                     /locus_tag="IW651_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0815111.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            10293..10817
                     /locus_tag="IW651_002406"
     CDS             10293..10817
                     /locus_tag="IW651_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0815112.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            complement(11134..12102)
                     /locus_tag="IW651_002407"
     CDS             complement(11134..12102)
                     /locus_tag="IW651_002407"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0815113.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
BASE COUNT         3575 a         2333 c         2498 g         3833 t
ORIGIN      
        1 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc
       61 cattttcaca aaatttttct taatactcga aactaagcga ctttttcaca acctcctgat
      121 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg
      181 agctattttt atttggctgt tcttattact tgatgcaggg cagttttttc acaacctctt
      241 catgctgtta aatggcttgc ataatgattg ttagtaatag ccccaataca taactggcat
      301 tgaataagac catatttttg atggaatagc tgaaactgat aggctggggt aacgtttgct
      361 tgaattgatc caaatgcttc ttcacaattg gcaaggtcaa gaaggtgacc aagatcggcc
      421 attcatagat accgctcaac aaccccatta aaatcacgaa atagcaagca tacatcaata
      481 gctggaataa gactgttccc gcaggacgtc cgatataaaa aactagtgta tagcgatgat
      541 tttcaatatc acggtcaaga tcccgcaaat tattagccaa catgatattg gcaatcgtaa
      601 acaccaaggg taaggaagct aatacgattg ctgctatagc ccaaagattt cctaccaaat
      661 caaattgtcc agatgaaaag ttgactgtca gatcatagac atctgaaccg aatgtattga
      721 cataaacgac catggcaaaa attccaaggc ccatagtcac cccactgaaa atttccccta
      781 aaggcattcg agataaagga atcggtccga atgtatagaa tactccaata aaacaacaga
      841 taaatcccat caaaagtaaa agccaacccg tattgatcgt tagaataacg ccgataacaa
      901 aaacaaatgc aatcatagct aaaatcatgt ttcgaatcaa tacaggagat agatttctgc
      961 ggccaatgac attttcttca tacttatata cttcagaatg agccttttca taatccatgt
     1021 aattattgat agcggtagtc gctaaatcaa aaactagcat tccaataaaa aataaaaaag
     1081 tgtttagcca atgtatctca tgaaaaaaag cgatagagaa taatacccct accgcaaaag
     1141 gaaatacact agccaccttt gttcgtattt caactacttc taaaaagatt tttagtgtca
     1201 tggttcgtac tccttatttt agttcagaac ggtcacccat cgtgtaatgg gagtcctccg
     1261 ataatttgaa ggtgtctttg attggatcgg tcacatatac tttatcgtct tttgtcacga
     1321 agatcgcttc cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct tttttcacac
     1381 ccattgaaaa gactgctgta gacaacccgt caccatcaat tgatttatct gttatgatcg
     1441 tgacacttgc gatatcattg tcgaacggat aacccgtttt tggatcaaac aaatgatggt
     1501 attttttgcc atctacttct aaataacgct cgtaaattcc agaggtaact aatgttttgt
     1561 tccgttcttt aatagaacct aaaacagatc cgcgagccat gttcggatct tggatgccga
     1621 ctgtccagtc ttggttttcg cctcttgggc tgtggcctaa gacatagaca ttaccgccca
     1681 agtcaacgat ggcagtggtc actccttgtt ttttcaagac tttcactact tcgtccgtaa
     1741 tgtaaccttt ggcgattgca cctaaatcga tgatcattcc tttttctttt agataaacag
     1801 ttttttcttt gtcatttagt tcgatcttat gataatccac taatttcaat gcctgatcga
     1861 tttcttcttg ggaaggtttt ctggcgtcat caaaaccgat tcgccataat tgtgtgattg
     1921 caccaatcgc catatcgaat ccgccttgag aatcttggct atattcgtag gcccgcttga
     1981 ctaacgtgta gacgtcatca gaaactttta ctggtttgat cccagcttgc tcattcactt
     2041 catcgatctc agaacctttt tgattgatcg tgattttatc accaagttct tttactcgat
     2101 cgaaggcagg tttcaatgct gattctttgc cttcatcata cacacggata cgaacgtaag
     2161 tccctaacaa gaactgttca tcagaatatg gatctttcaa tatcttggta gctgctgaac
     2221 tttcagtggt tgttttattt gctgagttgc tgcagccagt tagcaccata aaagagatgc
     2281 ccagtgtgat taaggaaaat agtagttttt tccgcatctt tttcctctct tcattttgaa
     2341 aggtgagacg actgcctaat ctggctatcg tcccaccctc caacttttat tagtatgatt
     2401 atgtgattat ttttctgtta cgatattatc tacttcgatt gtttgagtgt cgcctttttc
     2461 agctgcattc actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt gtgtagcacc
     2521 tgtaacaact tctacattag caggtgagcc atcttcttcg cccattgctt tgacgaattc
     2581 gtcgttcaga gtagggatgt aagtttttgg ttctgtaccg ctttttgctt tcatattttc
     2641 attatattct gtatcatctt gttttgattt accgtctttg tcgatataat caaagtttga
     2701 ttcagttact ttgccgccag caactgtcat ttcgaattgc acacggtagc cgttagagta
     2761 gttcttttct tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt cgatttcgat
     2821 tgtatctgtg ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt tttggaagct
     2881 ttcagatgag tgagtagctc cagttacgac ttccacaccg cttgcgcttt gtgcttttaa
     2941 gaattggtca ttcaactcag gaatgtattt ttctgggttt gtgccagatt ttgcttccat
     3001 gtttttgttg tattctgcat cttttgtttt tgattcgccg ttttcattga cgttatcata
     3061 ttttgattca gtgattttgc cgtcttttac aaccatttca aagactgcac ggtaaccatt
     3121 tgaataattt ttttcttcta atttatatgt gccgtcttgc aaatctccgc cagctacaac
     3181 ttttgcagat gattctgttg tgctttttgc agtcgaagat tgtgctgtag atgattcaga
     3241 agaagagttt gagcttccgt tgtcatctgc gccgcaagcg cctaacacta gagctgaaaa
     3301 tgctaatacg gcaaatccag ttacaaaacg ttttttcttc attttaatac cccaccattc
     3361 catttctagt ccacaatttt tctttgtgat tcattataca ttctaatctt tttagcctat
     3421 aagtcaacta tttttgataa ataacccatg taatatttca aacgttttct ttcagcaaat
     3481 ttcgtgaaaa aacgctttat tttaaaattt gaaaaaaatc caaaaggagt tagacaaaag
     3541 aacagaacat ctatataata tatggagatt gtgtaataag tatcaaacta aaagaattat
     3601 catatattca ttatatagat agtctataag gagcggatga tcaatggcaa agcaaaatat
     3661 tgtcgttgta ggagcaggat atgccggtgt ttcagctaca aaatacttgg caaagaaact
     3721 gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat tcgtaccata ctatgatgac
     3781 cgagttgcat gaagtagctg gcggacgtgt agaaccagaa gcaatccaat acgatctgca
     3841 gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca gacactgtaa tcggtatcga
     3901 taaagaacga aaaatcgtca agaccaaatt aggttcctat ccttttgatc agctgatcct
     3961 tggaatgggc ggagagccga atgactttgg tacacctggt gtcaaagcac acggattcac
     4021 cctttggtca tttgaagatt ctgtaaggat tcgggaacat attgagaaga cagtagcaaa
     4081 agctgcattg gaaccagatg atgaactgcg taaagccatg ctgactttcg ttgtctgtgg
     4141 ttctggattt accggtattg aaatgattgg ggaactgatc gactggaaaa atcgcctagc
     4201 caaagattat aaattagatc caaacgaaat cacactgatg gtcgtagagg caatgccgac
     4261 gatcttgaat atgttggatc gaaatgatgc tgcaaaagct gaacgctatt tgcgtaagca
     4321 caacgtggaa ttattattga atgcaccaat cgtagaagtg gctgctgatc atatcaaatt
     4381 gaaagacgga cgcaacattc cgactcacac actgatttgg acagctggtg tcaaaggaac
     4441 ttctgacgct gctgactttg gattagaatc agcacgcgga cagcggttga tcgccaatga
     4501 atatatgcag gcaaaaggct acgaagataa aaacatctat atcattggtg atttagtctt
     4561 ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt caagctgctg aacaaacagg
     4621 acacacagcc gcagcgaata tcgtggcttc gatcaaaggc ggagaaaagc ataaatttaa
     4681 aggaaactat caaggattta tggtttctat tggtgccaaa tggggggtag ctaacctatt
     4741 tgataagatc catttaagcg gatttttagc tatcgtaatg aaacatatcg tgaatttgaa
     4801 atacttcttt gatattcgtt ctggttatta tatgttccaa tatttaatgc atgagttttt
     4861 ccatatcaaa gatgatcgca acgtgactcg aggacattct tctcgttacg gcaatgtctt
     4921 atggagtgtg ccacttcgta ttttctatgg tttagtttgg ttcgttgaag cttccaaaaa
     4981 aatcattggg aatggtgaag tgttcaaacc aagtacttgg tttggtgaag gatcatggtt
     5041 cactggaaac agtgtgttcc cttggacatg ggaatacaat gtaacagatg ttgccagtgg
     5101 tgcttctgct gctggtgatg caaccacagc tgccagcgga gcaggggcaa gtgccggaga
     5161 agcagcgact caagctgcac attttggttt gagttatgca tacggtgaag aaccaatggc
     5221 tgtgattgag aagtcaccag actggttcac aaagatcatg caagtgatca tgccaaacaa
     5281 tgatgtagca atgttcttcc aaaaattcat ggtattcttt gaaattgcct tggcattagc
     5341 attgattgct ggattattca catggctatg cagtgcgaca acgattgtat tggtgatcac
     5401 gttctgctta tcaggcatgt tttactgggt aaacatttgg ttcatttttg ttgctttcgc
     5461 attgatgaac ggttccggac gtgcgattgg gttggatcgc tgggtaattc catggctaca
     5521 acgtacacta ggtaattggt ggtacggtag accaagttcc cagtacggca agaaataaat
     5581 agataaagaa cgttatttga atgcaagatt tcaatagaaa ggggctgaga caaaccgtca
     5641 cagcctcctt tttatgtaaa attaaaggaa taagatgccc gagataagcc tccatgagca
     5701 agacacaggt gtcacaacct ctgttacaac ctcttgttat aaacggtgtt ccagaagtag
     5761 cttcttcgaa aaataagccg aaatttcaca aaaacttgaa aagtaatttt cgtaaattcc
     5821 ttcttatttc ttgaagttgc ccacttctgt cacaacctct tgttataaac ggtgttccac
     5881 ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca tggaaagctg ttttctattc
     5941 ttcgttctta ttactcaaag caagacacag gtgtcacaac ctcttgtttt tatgctaaaa
     6001 ttgggtgagt taatctggaa aaaggagaac aaaaatgagc cttaaagaat ttgctgaaaa
     6061 aagtctgctc aaaccttggg atgtttttat tattttgctt ttgactgttc tttctttttt
     6121 accagtcatg atttttggtc ttcagcaaaa caccgcaaca gcagaaaaag aagcggtact
     6181 gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg gctggagaag agagcaacac
     6241 atatacttac aaagatgaac acggagatta taacttgatc gagatagatg gcgatcgagt
     6301 acggataaaa gaagcagatt gcgatgacca gatctgcgta cgtcgaggct gggcaacgaa
     6361 aaatggcgaa accatcgtct gtttgccaca taaattagtg atcgaagtcc gttcaacaga
     6421 tggaggtgac gaagacagtc tgatttatta gaccttcaca caccatgagt aaattacaaa
     6481 aaatgattta tatttcgcta cttgttgcac aaggagtgat cattggttta ttggaaaata
     6541 tgatccctta tcctttttct tttgcgcctg gtgcaaaact aggcttagcc aatctaatca
     6601 cgatcgttgc catttttacc atgaaaaaac aagacagctt cttactactt tggctacgac
     6661 tattcctgac tactttgcta ggcggaacga tttccacatt cctttatagt atgagtggtg
     6721 ctttgcttag ttattttgga atgttattgg tcaagcaatt aggaccaaaa cgtgtcagta
     6781 tcatcggaat tagtgcggcc ggcggtttca tgcataatgt cggacagctt ttgatggctt
     6841 ctttcattgc acaatcatgg acagtcatgt tgtacttgcc agtcctttca gttttaggga
     6901 ttttatcagg gattgcaatc gggatcgcag cgaattatct tttacgacat gtgcagacac
     6961 ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca aaaatggagc ccaatgttta
     7021 ccaaaaaata atcgtcacac aaggagtttt tttatgaagc tacatccaat gtgggaaaac
     7081 tatcctgctt taagtaaaga attgacagaa acactaaaac ggatggaaga aactgtccgc
     7141 ctgaaaaaca aacctgtcga acaagcaatc aaagaaacga tccatgccgg cggaaagctg
     7201 ttgcgtcctg cctatcagct attgttctca caatttggac cagagcaaga tcgagacaaa
     7261 gccatcgctt tggcagcttc tatcgaaatg ctccatacgg ccacactgat ccatgatgat
     7321 atcgttgatg aagcggatct gcgacgtggt cagcctaata tccgtagtca gtttggtaat
     7381 gtcgtttctg tctatgcagg agactattta tttgtctgct gttttaaact attgtctgat
     7441 tactccactt ccttgaaaag tctccaattg aattctcgca gtatggaaaa agtattgaat
     7501 ggcgaattag ggcagatgga tgatcgctat aattatgaac tttctgtaaa agaatatttg
     7561 gaaaatattt caggtaagac agctgaactg ttccaattaa gttgttctgt tggcgctttt
     7621 gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag atatcggact ttctatcggg
     7681 atggcctttc agattatcga tgatatttta gattatacaa aagagagcca ggaaatcggc
     7741 aaacctgtac tagaagacat gcgtcaaggg gtctattctt taccgttgat ctactctttg
     7801 caaaaaaaca aaccaaagct actaccttat ttagagaaga aagcggcaat gacagaagaa
     7861 gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag gcgtggaaga agctaggaag
     7921 ttagcggcct cttacacaga aaaggctttg aaggaaataa agaaattacc tgccacctct
     7981 cttcgaacaa aagagaatct cttttccctt acccaactga ttttggatcg caaagactga
     8041 atagggaaag gttcgatacg taaataaatt tctcagaagg aaatctacgt gttttttgac
     8101 aaattgaatt gtagttcttg tatttatgat aaaaagcaaa aataaagagc aacgtccgtt
     8161 agcgacgttg ctctttttta tcttgaatat ttttttacct tttcgatttc ttataactca
     8221 taaaagtgat tcttaaaccg attacaagta atataacgcc tgcgagtgtt atccaattat
     8281 tcgattgact tccagtttta ggaagtgctc tttttgattt gtttccattg tcattgcttg
     8341 ttttattagt taagttaaag ttcgtcctgt tatccgaact tgtttttgag aaggagacac
     8401 ttgcatgacg tgtatcgctt gtggtagacg acgtgtctgt actatcgctt gaagttgatg
     8461 aatctgagct atcacttgtg gtagaggatg tatctgtact gtcacttgaa gctgacgtgt
     8521 ccgtgctatc acttgtagtg gtagatgaat ctgagctatc acttgtggta gaggtagtat
     8581 ctgtactgtc acttgaagct gacgtgtccg tgctatcact tgttgtggta gatgtatccg
     8641 tactgtcgct tgaagctgac gtgtccgtgc tatcacttgt ggtagaggta gtatctgtac
     8701 tgtcacttga agctgatgtg tccgtgctat cacttgtggt agaggtagta tctgtactgt
     8761 cgcttgaagc tgacgtgtcc gtgctgtcac ttgtggtaga ggtagtatct gtactgtcac
     8821 ttgaagctga tgtgtccgtg ctatcacttg tggtagaggt agtatctgta ctgtcgcttg
     8881 aagctgatgt atccgtgcta tcacttgtgg tagaggtagt atctgtactg tcgcttgaag
     8941 ttgatgaatc tgagctatca cttgtggtag atgtatctgt actgtcgctt gtgtctgtcg
     9001 tatctgtacc attgtcgctg ataaatattt cgttaggatc ccaacgattg gtagctgcat
     9061 ctaaaatcac atttgttcct ataatcgttc cgtcttggtt ttgagtaatg cggatagtag
     9121 cattaggcgc aaggattgtt ccttgaaaag gagcactgat tgtcaaatca gtcatctcat
     9181 ttccaaaatt ccaagagata ttcgcatccg aaaaatcttc tgtttcatga tttgaacgat
     9241 cattgtattt gattggtgac tgtacgttca atgcagaaga actgttgatg acattaaata
     9301 caactacttg atcatttgga ttaatgattt gaagtggcgt attcatcgtt aacacttctg
     9361 catcgatatt taccaataaa aacccggtat ctgataaatc agttaaatcg attgtccgat
     9421 tgttcatgtc tggaaaatct gcagcagtat atgttttagc tggagtgatc gttgcaagat
     9481 catttgctgc tacattcaat ttagcaaatt cctgctcgaa atcaatatat tcatgcccag
     9541 cacgatcttt ataaaaatca tctggagtta agtgatcaag cctggatcca ttgactgtag
     9601 gatgaccatc ttgaagtgct acatccacgt tactgccaac gacaaattta ttcattcttg
     9661 tgctacctgt aggtatgcct gaagaattag cgaaagaatc agttttttgt atataattta
     9721 tttcttggtt caataaacct tcatgaagct ctgtaccaaa attatttttt gcatcaagtt
     9781 catgtgttgc tatatttccg tctgtatgtg cattcacagt tgtcttattt ttagcaaata
     9841 cgtggaattg actagcaatt cctaacaagc catagttacc agttagatca ttataaatat
     9901 tcccagggtc tttaggaatg tatgtttccg ctgaaacggc cacaccatac gaaaagatat
     9961 tgaacaacaa agcaactagg actagtaaac ttgccctttt tttcattttt ccccttcctt
    10021 tcctttcaaa cattttccta ttatactctt tttctcctag tcttaaactg ttcgtttgaa
    10081 aaatcattgt ttatttaaag aatattaatg aaacgttcat attgttgctt cttgttcaat
    10141 tgatagttcg acactatctt tttctttcaa tctccaaact attgatgatg gtgactgtta
    10201 aagtgtaaaa aataagggtg tgtagtaaca tataagtagg tatcctagaa ttaggaaaaa
    10261 gtaagtagga taaataaaaa ggaggaaaat aaatggatta tttagatatg ctatgcgaga
    10321 gtgtggatcg ggcatacgat cggtttgaac gtttgtttga aggcgtctct attgaggagg
    10381 ccaatcgttt tccagtggaa gatagagccc cgcagatcaa atcaatgact tggctggctt
    10441 ggcataccgc tcgagaatta gatttccaga ttgctgattt agcaggaact tctccaattt
    10501 ggcatagtca agactgggaa agccggtttc cgatgacgat agcagataac gaacaagatt
    10561 ggaagcatag tctggaacaa gcaaagcaga tccaagctaa tcaaatagaa gatctgctag
    10621 gatacttgcg aggagctgct gattttgcta tttcttactt aagaaggctg gaaccttccg
    10681 agctagatga agtaattgac gagaactgga caccaccagt cacacgagga atgcgtctcg
    10741 tttctattat tgatgatgct gctatgcact ctggtcaggt gatctatgcg agacgtttgt
    10801 tgggattaag agattaaggc actagctaaa aaagaactta cggcaaaata agtcgcaagt
    10861 tcttttttgt ttttatcgtt acccatggtc tatctataag attgaaaaag gaagcggcct
    10921 gctatctctt ttgatcgtgt aaactgatga ctctcttgtt ttttgcattg attcatttaa
    10981 agaaagtgta ccatgcttta tagatatttt tagaatacct ttttctagag gatagagaaa
    11041 ctgtcagctg atagaataag gagagtcttt tgaagagaat tcagaaaaaa ggacactgta
    11101 tgagatcagg tgtccttttg tgaaatctga taattattgc gtacggatag atgtgagact
    11161 gatttcgcca gaagacaaac tcatcttctg ttcatttgtt tggacgatca gttcgccatg
    11221 atcaccgatt tcttttgcaa gaccgacata agtcgttcct ttttgattga acgacacggt
    11281 ttttcccaat acgaatgatt tttctttata gaagtccaac gctttttctg ggtgagaaag
    11341 ataagtgaac aattcttgcc agattttgct gattagctga ttccttgtga cactagcttg
    11401 tccgtcggga aagacagaga tggctttttc ttggagatca gctggaaaat gattttgagg
    11461 aacagaaaaa ttaagcccga tcccaatgat gatatgactg atcctgccgc tttccatatc
    11521 actgactgct tcagataaaa tcccacagac tttttttcca tccatgtaga tatcattgac
    11581 ccatttgatc gaagcagaac gaccggttac agattcaatg gctttcacac aggcactggc
    11641 tgcgatgatc gtatattgag gcaattcatc aaaccgtttg tctggatgga gcaagagact
    11701 catgtagatt cctcgtccag gttcagcgaa aaaaggccgt ccaaaacgtc cacgagcttg
    11761 ttcttgtgta tcagcaacaa ataacttcga aacagaatct ccttgagctg ctgccagttt
    11821 ggcatctttc atcgtagatt cagaagtcaa catcgtttgt actttcaaat caggaataaa
    11881 cgaccgtagc tcttgctgga tttcttttgc atctagtacg tcagagggca ggtaacgata
    11941 ccctgtaggc ccgtgctcaa aacgatagcc tgccttttcc aattctttga tggctttcca
    12001 aatacttgtc cgggaaactt ccagttgctg tgccaacttt tctccggaaa gaaaatctgg
    12061 tgtctttttc aataattgaa gtaccttgtc tttcgttgtc atggcatcaa gctcctttcc
    12121 catccattct atcaggaaga aggagagaac aaaatagttt tcgaagaaac aacggttgat
    12181 acataaagca aaaacgaaag aaggaaagag aaaaaagaag agggtgggcg ttcattttt
//
LOCUS       DACEJR010000082        12229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEJR010000082 DACEJR010000000
VERSION     DACEJR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.083"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1108)
                     /gene="hflX"
                     /locus_tag="IW651_002408"
     CDS             complement(<1..1108)
                     /gene="hflX"
                     /locus_tag="IW651_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0815114.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQ"
     gene            complement(1110..2027)
                     /gene="miaA"
                     /locus_tag="IW651_002409"
     CDS             complement(1110..2027)
                     /gene="miaA"
                     /locus_tag="IW651_002409"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0815115.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2031..2768)
                     /locus_tag="IW651_002410"
     CDS             complement(2031..2768)
                     /locus_tag="IW651_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0815116.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2859..3386)
                     /locus_tag="IW651_002411"
     CDS             complement(2859..3386)
                     /locus_tag="IW651_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0815117.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3566..4159
                     /gene="clpP"
                     /locus_tag="IW651_002412"
     CDS             3566..4159
                     /gene="clpP"
                     /locus_tag="IW651_002412"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0815118.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4263..4748)
                     /locus_tag="IW651_002413"
     CDS             complement(4263..4748)
                     /locus_tag="IW651_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0815119.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(4901..5098)
                     /locus_tag="IW651_002414"
     CDS             complement(4901..5098)
                     /locus_tag="IW651_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815120.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5193..6953)
                     /gene="efrB"
                     /locus_tag="IW651_002415"
     CDS             complement(5193..6953)
                     /gene="efrB"
                     /locus_tag="IW651_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0815121.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(6950..8680)
                     /gene="efrA"
                     /locus_tag="IW651_002416"
     CDS             complement(6950..8680)
                     /gene="efrA"
                     /locus_tag="IW651_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0815122.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9073..9285
                     /locus_tag="IW651_002417"
     CDS             9073..9285
                     /locus_tag="IW651_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0815123.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9287..10966
                     /locus_tag="IW651_002418"
     CDS             9287..10966
                     /locus_tag="IW651_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0815124.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11220..11420)
                     /locus_tag="IW651_002419"
     CDS             complement(11220..11420)
                     /locus_tag="IW651_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0815125.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11570..11731)
                     /locus_tag="IW651_002420"
                     /pseudo
     CDS             complement(11570..11731)
                     /locus_tag="IW651_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12111..12184)
                     /locus_tag="IW651_002421"
     tRNA            complement(12111..12184)
                     /locus_tag="IW651_002421"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12148..12150),aa:Ile,seq:cat)
BASE COUNT         3577 a         2640 c         2131 g         3881 t
ORIGIN      
        1 tctgtccttc gttgcttggc acagaaagtg tataaggggt cattagttcc atcagctgac
       61 gtttgattgc ttcgatcaac gccatcttac cttctttcgt ctgagtagaa atcaatacat
      121 ttgggaatag cgtaggcgtg aacatcgctg ggtcgatctg gtctgcctta ttgtaaacag
      181 tcagtatcgg catttctttc atattcagtt catccatcaa ttttaagaca gtctgctcct
      241 gtaaaatacg atctggagaa ctagcatcga ctacatgtag caaaagatcc atattttgac
      301 tttcttctaa tgtagaatgg aaagcatcga tcagttgcgt aggcaggtct tggatgaacc
      361 cgacggtatc cgttacagta atctcaaatc cttcagcaaa acgccatctc ttagtcaatg
      421 gatctaacgt tgcaaacaat tgatctttcg aataagtatc cgcttgtgtc aatagattca
      481 agatagttga cttcccggca ttcgtgtagc caataagtcc aatctgaaaa atctcgctat
      541 tttttctttt ttggcgattg cgctctcgat gagcttctac ttctttcaat tctttcttaa
      601 cagcaaaaat tttattccgt atatgtcggc ggtctgtttc taactttgtt tcacctggcc
      661 ctcttgttcc gatcccaccg ccaagacgag ataatgtttt cccttgccct actaaacgag
      721 gcagcaggta ttctaactga gcaagttcaa cctgcagctt cccttctttc gaacgagcac
      781 gcatcgcaaa aatatctaag ataagctgca cgcgatcaat taccggaaga ccaacagcat
      841 cggtaatcag ttggctctgt ctcggagtta gctcatgatt aaagatgatc aaatccgctt
      901 catgcgcatc cgcttgttga actaattctt gtaatttccc tttaccgata atcgtttgcc
      961 gatccacttg tggtctcttt tgtgtaattg tgaatacgac ttctcctgat gcggtattcg
     1021 tcagctgaac tagctcttcc attgactctg aaaagtaacg ctggtttgct tcggtttcta
     1081 cccctacgat gatcactttt tcttccattt tattccgcct ccagccattt tttgattgtt
     1141 tgctcaatct gacttttttg atttggctca cttataagat caaaccactg tgcatccaaa
     1201 cggttacgaa accacgtcaa ctgacgtttc gcgtatcttc tcgaatgtag tttgacttgt
     1261 tctgttgctt cttccaatgt ttctttccct tcaaaataag ggaaaaattc tttatatcca
     1321 ataccttgac tggcctggac ttctccaagc tcatacacct tacgcgcttc tgctactaaa
     1381 ccttctgcta gcatcaaatc gactctttga ttgattcgtt cataaagaat actacgcttc
     1441 gtatctaatc caatcatgag gtaatcatac aattgctttg gctcttcttt aggtgccgtg
     1501 atactatgtc ctgtcgtctc taaaacttcc agagctctta tcactttgcg ttgattgttc
     1561 cagtggattt tctcagcagc tgccgggtct ttttctttca actgattcca taatgcttgg
     1621 gaaccaattg tttcagctaa cgtttcatac ttttttctaa tctcaggatt cgcttcttct
     1681 gtcgctccta actgataatc atatagaagg gattggatat agagacccgt accaccagct
     1741 atgataggta ggtgccctcg gttcgcaatc tcggtaatca attgtcgacc tgaatgctga
     1801 aaatcagcgg ctgagtaacg ttgagtaggt tcgatcacat caatcaaatg atgcggaaca
     1861 ttattcatct cttcttcttt gatttttgct gtaccgatat ccagattttt atagatttgc
     1921 aatgaatcac cactgatgat ctctcctcca aatagttgtg cgagttccac acttagcgct
     1981 gttttaccga ctgctgtcgg accgacgatc actaatactt tttccatatt ttacctactt
     2041 ttcgttgatt gtttccaccg aagagctttc tccggaaaat cagttattat tccatctatt
     2101 ttgagttctt gacatttggc catttcttct tcatcgttaa tcgtccatgc acgaattgct
     2161 tttggaaaat cagctgcctg gactggatgt tcgaacaacc aagtattttt tggatgtact
     2221 ccttctatat aaggggtttc tgctgctagt tggatctttt tatcagaagt actcatcaat
     2281 aagtccagct gtatctccgg ttccagtaat gcgatttttt ccaaagaatc caaatgaaag
     2341 ctgcaatatg catgttgaaa aggccaatct ctacttttta atgtttcggc taatttttct
     2401 tcgataccgg gatattcata ctgatcagtt ttaatttcaa tcattagcat ccctcggtaa
     2461 tttttttgga ggagcagtcc taacacttct ctcaattcag gaactttagc tgctggaaat
     2521 tctttgctga atttgccccc tgcattcaat tttttgagtt cttccaatgt tttttctcga
     2581 atcagccctt taccgttagt cgtccggtcc acttcttcgt cgtgcataac gatcaaataa
     2641 ccatctttgc ttagatggac atctagttcg ataccatctg cacctactcg tacagcttct
     2701 gcaaatgcag gaagcgtatt ttctgggtgg gtgccgctgc ttccgcgatg ggcatagatt
     2761 tgtaacatct ttcttcactc cgttcagttc tattgtatca ttaagaaaaa aagaaagcat
     2821 taccacgctc tcatgtgcaa cggcaatgct tcaaaatatc agctccaaac atcatctttc
     2881 gagaagaaac caatcggctg gcgcgtcata gtcttcaact gagctttttt ttcattgatc
     2941 atttcagtca gactatccgt aaaattctct aaaatcacgc gtccgccttt ataagcatat
     3001 ccaccaatct tcattgtttc atctggagat aaaatcactt ttcgatggga gatattctta
     3061 aatagctcgt ctaaaatata atttggcaga taaaaactat tttgcgctga aaatgattgg
     3121 agatacgcaa aatcgtcaat cgtgatataa caatcatcaa atgcatcaaa gctgacatat
     3181 tcttccatca gcgttgtccg tttttgcaag atctcgttga tctttttatt taattgattc
     3241 agcgtctcga tttctttgac tttcaattcc aattcacgtc tacgctgttc tttattttcc
     3301 aagtgacgca tttgcagtat catgccgata tagacaacta cagcacctgt cagcactccg
     3361 ataaggccag taataccaac tatcaatctg ttgttcctcc ttgttttgtt tctctgctta
     3421 tcataccata aagcgggttt atgcgactaa tttgcctaag ttttttattt gacctttttt
     3481 gaccaaaaat gtatactaca ttttaggtat tagaaactaa attatggtac actatttcta
     3541 aacgaaatca aggaggaact ctattatgaa tttaattcct acagtcattg aacaatcttc
     3601 acgaggtgaa agagcttacg atatctactc acgtttattg aaagaccgta tcatcatgtt
     3661 aagcggccaa gtaacagatg atcttgctaa ttcgatcatc gcccaattgc ttttcttaga
     3721 cgctcaagat tctgaaaaag atatctatct ttatatcaat tctcctggtg gttcagttac
     3781 agcaggtatg gctatttacg acacaatgaa ctttgtcaaa gcagatgtcc aaacaattgt
     3841 tatgggtatg gctgcttcaa tgggaagctt cttgctgaca gctggtacaa aaggtaaacg
     3901 atttgcttta ccaaatgctg agatcatgat ccatcaacct cttggcggcg ctcaagggca
     3961 agcaactgaa atcgaaattg cggcacgcca tattttgcaa acacgcgaac gcttaaacaa
     4021 aattttagct gaacgcactg gacaaccgct tgaagtgatt gaaaaagata cagatcgtga
     4081 caactatatg acagctgaac aagcaaaagc atatggattg atcgatgaag tcatggaaaa
     4141 cagttcttca ttaaattaat ctgtttcaaa ggaagctttt ttaagctctc ttatctaaac
     4201 caaataaaaa aggatggtca actcaaactg accatccttt tttattttat ggatgctact
     4261 gcctatcttt gctccttgaa ttttccttgc gcatgcatcc gacctatctc gacataatgc
     4321 tggatgtttt ctttgttttt ttgtatttct tcttttgtca aaggacggat cactttagct
     4381 gggcgaccaa aagccaagac attaggagga ataacttttc cttctgttac taatgatcca
     4441 gcaccaatca aactattctc accaataacc gcatgattca atatcgtggt ccccatcccg
     4501 atcagcgccc ctttttcgat tgtacagcca tgtagcatgc actgatgtcc taccgtcaca
     4561 ttctccgcaa tatctacagg tgcatcatga tcgacatgga tgattgtacc atcttggata
     4621 ttcgttcttt gtcctatttt tatccaatta ctgtccccgc gtaaaacagc ttgataccag
     4681 atcgtcacat cttctgacaa tgtcacatca ccaatcactg tggcatttga tgctataaac
     4741 cgttccatat ttatttttct cctttcattt tttgtagaaa ctaatcctat tgaataaagg
     4801 tatcatagca tatatttatt tcataagaaa aaaaacaggt aaaacctgct caggccagat
     4861 gtctatttac agtatctggt aactgacgtt ttacctgttt ttattgtaca tctatatttt
     4921 cttctattac tttttttaaa attaattcgt acacttctat ttgtgctgcc aagcgttcga
     4981 tttgaaactc attttcaaaa aaatcagctg aatcaaaagg cttcgtctga cagacgtgcg
     5041 ttaaacgatc aacttctctt ttggcttcag taattttttc atttaaaaac tgttccatct
     5101 ccgtgtcctc acttttcttg ataactttac tttaccatta tcaagaatag agaacaaaaa
     5161 ataagtttta aactgtttta ttcttatatg acttattctg cttcctcagc aaattggctg
     5221 ttatacagtt tttcataaaa tccaccttgt gccaataaac tctcatgcgt tcctttttca
     5281 atgatctgac cttgatccat cactagaatc agatctgcat cacggattgt cgataaacgg
     5341 tgagcgatga cgaaacttgt ccgtccctcc atcacgcgat ccattgcttt ttgaatcaac
     5401 gcttctaaac gggtgtctac tgaacttgtt gcttcatcta aaatcaagat tttaggatcg
     5461 gagaccactg ctcgggcaat cgtcagcaac tgtttttgtc ctaaagacac attgtctccc
     5521 tcagaattga tggccatatc atatccatct ggcatcgttc ggataaaatg atctacgttt
     5581 gctgttttcg cagcatcaac tacttcataa tctgtcgcat caagctttcc gaagcggata
     5641 ttatctgcaa tcgttccttg ataaagccat gcatcttgca agaccatacc aaatagcgaa
     5701 cgcacatcac tgcgactcat tgatttggtg tcgattccat caatccttat tgctcctttg
     5761 tctacgtcat aaaaacgcat caatagattg atcaatgtcg tcttgccggc accggtcgga
     5821 ccgaccacag ctacggtttg accagctttc acttcaaaat tcaaatcttg gattaatggt
     5881 ttcttcggat catatgcaaa atcgacatga tcgaatgtca catctcctaa aatcgtttct
     5941 ggcagtgaag catctacttt gttttctttt tcttctggct catctaaaat ctcgaataca
     6001 cgtttcgttg ccgctgatgc actttgcaaa actgaagaca attgtgtcac gttccccatt
     6061 ggctgactga tttgccaaat atattggatg aatgcttgta gttgaccgac aacgatcgcc
     6121 ccagtaataa cataaaagct tcctagaaca gccataccaa tataagtagc ataggcagtt
     6181 aattgaacta gaggcatcat caagccagaa atgaatgctg cacgaaaccc aaagtatttc
     6241 aagctgtgat taactttttt gaatccttcc aatgtttctt tttcgcgtcc atatactttc
     6301 aatacactaa aacctgtcat attttcttgg acgtagccgt ttagctcacc aagagaattc
     6361 tgcatgcttt gaaaatattt ctgcgaagca ttgatcactc gttttgagat ccagatcgat
     6421 gttggaatca tgatcataga gatcagcgcc atcatcggct ggatataaaa catcattgct
     6481 gcagccaacg taattcccaa tatcgcattt acgataccaa taaaagcttg ttgtaaagca
     6541 ccgctgactg catctacatc attcgtcaca cgagacaaaa tatttccttg ctggttttta
     6601 tcgaaatatg aaaccggaag acgattgatt ttttcttcta tgtctcttct caaatcatgc
     6661 atggcacttt gaacgacatt tgtcatcaaa acaccagaaa gtaattgcga aatacaatac
     6721 cctattccaa caactgtgat ccaaatgaga catttgaata tataagaaaa attgattgat
     6781 tcgccattgg caatattacg gctgatttct gtagtaggaa gtcctgtaac atatggcaaa
     6841 gccacgttga aagccactgt cagaactgta aataggatca ctaaaagaaa agtctcacga
     6901 tacggtctga tatatcgagc taaacgtttt aaggaagaaa aggttttttt catgctaatt
     6961 cctcctttga taattgagaa gcggcaatat cataataaat cgggcatttt tccaacaact
     7021 cccgatgcgt ccccataccg acgacttctc cttcatttaa tacgatgatc ttatctgcat
     7081 gcatgatcgt tcccacacgt tgtgctacga tcaatacagt agattctgtc gtttcttttt
     7141 tcagacgagc acgtaacttc gcatccgttt gataatctaa tgcagaaaag ctgtcatcaa
     7201 aaatatagat ttccggacgg cgtatgatgg ctctggctat tgccaatcgt tgtttttgtc
     7261 cacctgaaaa attggaacca ccttctgata aatgttcatt aaatccatct ggttttcgag
     7321 aaatgaattc tgaagcttga gcaatctcag ctgcacgttc catttcttct tgcgtagcat
     7381 cttctttccc gtatcgcaaa ttatctgcaa tcgttccagt aaatagcagc gctttttgcg
     7441 gaataaaccc aatcttatct cgcaacttgc ttagccgata atctctcacg tctactccat
     7501 cgatcaagat ctttccttca ctcacatcat aaaagcgagg gatcaattgg atcaacgtag
     7561 attttccgct accggtactt ccaataaaag caacggtctc tcctggagag gctgtaaaac
     7621 tgacatttcg tatcactggc tcttgcgaat ggcctggata agcaaatgtt acattttgaa
     7681 attcgatata ccctttagtt tttgtatcag atataccatt tggatcttct acaatcgaag
     7741 gtttggcatc tagggcttct tggatacgtc cagctgaaac agctgctctt ggatacatca
     7801 tgaacacact agcaaataac atgaatgaga acaaggcatg gaatatatac tcgataaacg
     7861 caatcaaatc tcctacacga agctcgcctg catcgatttg cactgccccg ctccagatga
     7921 tcaataccat tacgatatta aataagaaat aaaaacccgg ttgtgcaaca gccatcaaac
     7981 ggaataagct ttttgaacta gaagcataat tctcattgac tacagcaaaa cgtttttctt
     8041 caaacttttc attgacgaat gcacgaatca cacgcaatcc tgaaagattt tcccgcaaga
     8101 tactgttgat tttatctaag tttttttgtt gctttttaga taacggttca gacaccttag
     8161 caatcgctac gactgccaat aatagtaaag gtaatgcacc tactacatac aaactgagtg
     8221 tcacacttgt acgcatgacc atgtacaaac tgacaatgaa catcattggt gccataaaac
     8281 cgatacgtaa aatattttgc aggaacaaca tgatctgata agcatcgttt gttgtacgag
     8341 tgatcaatga agatacgcct agattttcgt attcctgatg cgaataactt tggatctttt
     8401 cgaacaaatc atctcgaata tctgctacga tattagtcgt aatcctcgaa ccaaaataac
     8461 ctaacaagat attcaaaata ataccgatta cagtaataac gatcataaat atcccttgtt
     8521 gcttcacata atcaaaatca tcattgcgga ttcctacatc gatcatacgt gctaaaatcg
     8581 taggaagtcc taattcaatc aaaataaaac caaatacgca tatgaaattg aaaaacaaga
     8641 atttcttata acgcatggta taatgccaca ttagtttcac tttctcatct ccttttaatc
     8701 ttttgttaca ttttcattac taaaaatgat agcacaacga gcctattaat agaacacttc
     8761 tctcataaaa aagtcattat gattatagcg ggatgataaa cattgatttt ttctttcaat
     8821 ctagttgatt tctatactta tttgctgttt tatgctagaa taacaattaa ttgttgtata
     8881 ttttttatac agaaggtacg gttgaaaaaa tatccgaaca gataagcaaa aacgaggctt
     8941 gtctctttga ctcgaatcca gtattctagt caaagcatat gaaaaaccag gtggcttgtc
     9001 ccctcgtttt gtgttgtcta aaaaaatcaa ctagctaaat cactgcttta cgcagataaa
     9061 ggagacaaaa caatgatcta taaagtttat tatcaagaaa caaaagtacg caaccctaaa
     9121 cgggaagaaa caaaatctct ttacattgag gcaaaaagcg atgtagacgc tcgccaacgt
     9181 gtggaagaaa ataccccata caatattgaa ttcgttcaac ttctagaagg aagtcatcta
     9241 gagtacgaaa aagaaaatgc tgatttctca ttagtggagt tttaatatga aactttccat
     9301 caaaaataac gagacaggcg tctttgctat tggtggatta ggtgaaatcg ggaaaaatac
     9361 gtatggggta caattccaag atgaaatcat tttgattgat gccgggatca aattcccgga
     9421 agacgattta cttggaattg actatgtcat tcccgactac agctacatca tacaaaactt
     9481 acacaaagta aaagccttgg tgattactca cgggcacgaa gaccacatcg gcggaatacc
     9541 ctacttgctg aaacaagcaa atattcctat ctatgctggt cctctcgcat tagcgttgat
     9601 cacaaacaaa ttagatgagc atggcttgct tcgagatgca gagctccatg agatcaatga
     9661 agatacgatc atccgtttta gaaagacttc tgtcagcttt ttcagaacga cccatagtat
     9721 cccagatgca ttagggatcg tggtgaaaac accttctgga aatatcgtag cgactggaga
     9781 cttcaaattc gatttcactc ctgttggcga accagcaaac ttgcataaaa tggctcgtat
     9841 cggggaagaa ggagtcttgt gccttttatc tgatagtaca aatgctgaga ttccaacatt
     9901 tactaaatca gaaaaaacga ttgggcattc tattttgaag atcttttcaa aaatcgaagg
     9961 acgtatcatt tttgccagct ttgcttctaa catcttccgt ttgcagcaag cagcagatgc
    10021 cgctgtggca actggtcgta aaatcgctgt tttcggacga agcatggaaa atgccatcgt
    10081 caacggtcaa cgtttaggat atatcaaagt tccagacggc acatttgtag atgcacacga
    10141 aatcaatcgc ttgcctgcca atgaaacgat gatcctatgt acaggttctc aaggagaagc
    10201 gatggccgcg ttgagccgca tcgcgaatgg tactcaccgt caaatccaaa tccagccaga
    10261 cgatacagtg atcttttcaa gttcacctat tcctggtaat acaacaagtg tcaatcattt
    10321 gatcaatttg ctgtctgaag ctggtgcaga agtgattcac ggaaaaatca ataacatcca
    10381 tacttccggt cacggtggac aagaagaaca aaaattaatg cttcgtttga tgaagccaaa
    10441 atattttatt cctgtccacg gagaattccg catgttgaaa gtacatgctt ctctagcaca
    10501 agatgtaggt gttcctgcag aaaactgctt catcatggaa aatggcgatg ttttagcgtt
    10561 aactagcgat tctgcgcgtc ctgctgggca ttttgtggct ggagatgtgt atatcgatgg
    10621 tagcgggatc ggtgatattg gtaatgtcgt tttacgtgac cgtcacctgc tttcagaaga
    10681 aggtcttgta ttagctgtcg cgacagtgga tatcaagaaa aaagaaatct tggctggtcc
    10741 agatattctc tctcgtggat ttgtctacat gcgagaatca ggagaaatga tcaatgaagc
    10801 tcagcgaatc ttattccacg ctttaagaga atgtttgaat ggacctgact gttcagaata
    10861 taaattgaaa gaagcgatga ctggcgcgct tcagcctttc ttattcgaac gtacagaaag
    10921 acatccaatg attttgccaa tggtaatgac accagatgaa gaataaataa gaggttatga
    10981 aatcagcacc ttgtcctgaa tataagagaa aacaaatgaa aaacatgaga agccattttt
    11041 gtttgttctt attccttgat acaaggtggc tctttcatag cctcttaaat ttatttactt
    11101 tttcatacaa aaaaatacct gccacaagag aaattgtctc ttgtggcagg tatttttgtt
    11161 actcgctttg ttctctcgta aaaattgatg tctaccatca atttgattta cagataaaat
    11221 taagctttta caacgtttgc agcttgtggg ccgcggtctg attcttcaac gtcgaaagtc
    11281 actgcttgac cttcttctaa tgttttgaat ccgtcacctt ggatagctga gaagtgaacg
    11341 aatacgtcgc ttccatcttc acgtgagata aatccaaatc ctttttcagc gttgaaccat
    11401 tttactgttc cgttttccat aataatatcc tcctcgtgcc aaaagcacta atttaaattg
    11461 taacattgct cgtgtacgat agaggtgcaa cagatgtttc aacttctatt cccaaacaaa
    11521 attacaatat aattgtacac ttgcttcatt ttttttgcaa gaaagcaact tacttctctt
    11581 ccatcattcc ttcttctctc aaactaaaat atcgtttgcg tccgatgatc aagtggtcca
    11641 gtacagtgat ccccatcatt tctccacact tttgtatcct tttggtaaaa ttcatatcct
    11701 gctgcgatgg aataacatta ccagaaggat aatataggtt agtacacgta taaaaatatc
    11761 ttttatattg acacaattta tttaacaagt ataataataa ttgaaagtga atttaaacaa
    11821 atatacacca taaacacaac gtacctgcga atacgttgtg tttttttctt tttcaaaaaa
    11881 agacgcttac cgctgcgaaa cagtaagcgc cattgtcggc tctaatgttt atctagccaa
    11941 tgactaaata atgccaaagt taaaccaatt aacaatggca agagcacgtg ctccaaagtg
    12001 aattcaaaca aatcatacac ctcctcccac gaaaatctct gtggaagcga cgagccgaca
    12061 ttttattata ccattattga attttatttt ttatcaaata gattaaatta tgtaccctgt
    12121 aggactcgaa cctacgaccg gacggttatg agccgtctgc tctgaccaac tgagctaaga
    12181 gtacaggttg ttgccacata aagccataaa caatcaacca gtagaaggt
//
LOCUS       DACEJR010000083        12167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEJR010000083 DACEJR010000000
VERSION     DACEJR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.084"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            105..692
                     /locus_tag="IW651_002422"
     CDS             105..692
                     /locus_tag="IW651_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815126.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(1171..3180)
                     /gene="metG"
                     /locus_tag="IW651_002423"
     CDS             complement(1171..3180)
                     /gene="metG"
                     /locus_tag="IW651_002423"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0815127.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            3448..3726
                     /locus_tag="IW651_002424"
     CDS             3448..3726
                     /locus_tag="IW651_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815128.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3823..4644
                     /locus_tag="IW651_002425"
     CDS             3823..4644
                     /locus_tag="IW651_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0815129.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4929..5939)
                     /locus_tag="IW651_002426"
     CDS             complement(4929..5939)
                     /locus_tag="IW651_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0815130.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5951..6904)
                     /locus_tag="IW651_002427"
     CDS             complement(5951..6904)
                     /locus_tag="IW651_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0815131.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6894..7964)
                     /locus_tag="IW651_002428"
     CDS             complement(6894..7964)
                     /locus_tag="IW651_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0815132.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7970..9013)
                     /locus_tag="IW651_002429"
     CDS             complement(7970..9013)
                     /locus_tag="IW651_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815133.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(9014..9955)
                     /locus_tag="IW651_002430"
     CDS             complement(9014..9955)
                     /locus_tag="IW651_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815134.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            10039..10386
                     /locus_tag="IW651_002431"
     CDS             10039..10386
                     /locus_tag="IW651_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0815135.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(10456..12117)
                     /locus_tag="IW651_002432"
     CDS             complement(10456..12117)
                     /locus_tag="IW651_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0815136.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3426 a         2455 c         2213 g         4073 t
ORIGIN      
        1 cttttttctc cactttcagt gaaaaactcc ctcttaagac ggatgtattt cttttttatt
       61 tttcgtatac taaacctacc ttttccacga aggaggaaca gtttatggat tttaagcgtt
      121 cttatccgaa attagcagat tatttatcca accgggtcga tttttcccgt aaagaacaac
      181 agcagtccca ttggcattgg ttcacattaa ttttatttgg attaatgact gttgcaacgt
      241 tatcacaaaa actaatattg ctgactttgt tcatcgcaat cgttgcattg atcaaaggtc
      301 ctgtgatgat cttgtggggg atgttgtatt cctttttggt tgggcttttt ccgcctatcg
      361 gtattttgct ttcagcgatt ttctttttat tgaatatcgg aacatttaca aagaactggc
      421 ggatgacctt agtcgggatc tatttttatt tctatccttt tggagtgatg gcacttagtg
      481 aagtcatgca ttgggataat cattggttta tagctggctc cttgctttta gggttgatct
      541 tgttacacgt gatgttgaca aagctctatc aacattatgg tatcggacgt acgattttct
      601 ggtatgtctt ttcgatccca tttgcactgc ttactgctct cttaccaagc cgtttgaaga
      661 caaaaataaa aggttaccat aaaatcaagt gataggattt ttacaaaata aagaaaacaa
      721 tcatcgaagt caaagccgat gattgttttt ttatctatat tttcaatcac acatataaga
      781 gcacaaacta attgaacata aagtgataga acttataatt aacacatcaa cgctactgtt
      841 agatataaat atagatgtaa taaagtcatt gcattcaaac tttatgaatg taaggacaat
      901 aaaaaagagt accagatgaa tcaattaaaa tctgcccgac cctttatcat tatgcttttt
      961 ttctgttttt acgattccta ttagtattat tctaaattaa caaaaatcag cgaagctcgt
     1021 acttcttttt ctaggattta ttttggatag ttctctgttt ctgtattata tgattggctt
     1081 caagtaataa gaaggaattt acgaaaattg cttctcaaat ttcggaaaaa gggggtatga
     1141 cacaactagt gtcatacccc ctttgaattt ctatgcgatt tctgagccat ttggcatgga
     1201 ttttggtgct tcgattactt gtaaagagcc atctggtgct tctgcagaaa ggatcattcc
     1261 ttgactgatt tgtccacgca tctttcgtgg tttcagattt gctacaatga cgactttttt
     1321 cccgatcaat tcgcttggat caggataaaa ttcagcgatt cctgacaaga tttgacggtc
     1381 ttggctgtct ccagcatcta gtcggaattg cagcaactta tctgcccctt ctactttttg
     1441 acagttgatg acttctgcta cttttagttc tactttttca aatacatcaa atttgatttg
     1501 tttttctttc gttgaaacaa gttctgtttc ttctggatcc catttgactg tatcttcgtt
     1561 tgtttgagta ccttcagaca ttttttcttg aataaaagca acttcttctt ccatatctaa
     1621 acgtgggaag attggtgtac cttttgcaac tacttttgtt cctgaaggaa attcgccaaa
     1681 gtgtaatcct tcaagattca ttgtttcaga gtccaatcct agttgattga agatcttctc
     1741 tggcgtttct gtcatgattg gctgaagtaa tatcgctgcg atacgcaaag attcagctaa
     1801 atggatcatg acactattca attcagcttt cttttcttcg tcttttgcta atacccatgg
     1861 ttctgtttca tcaatgtatt tgtttgcacg agaaatcagc acccagattt cagccaatgc
     1921 tgtattgaat tccatttttt ccattgcatc atggtaacgt ccgacaacgt tagcagccgt
     1981 cgtagacagt tcactatcaa atggagtgac aagtgaggca tagtcaggaa cgagtccatc
     2041 acaatatttg ttgatcatag caatcgttcg attcaataag ttccctagat catttgccag
     2101 atcataattg acacgggaaa caaaatcttc tggcgtaaag acaccatctg acccaaacgg
     2161 aacagcacga agcaaataat aacggagtgc atctaaacca taacgttcca caagcatttc
     2221 aggataaacg acatttcctt ttgatttaga catttttccg tctttcatca atagccagcc
     2281 atgcccaaat acttttttag gtagaggcaa atcaagggcc atcagcatga ttggccaata
     2341 gatcgtatga aagcgaacga tttctttacc gaccatctga acatctgcag gccagtattt
     2401 ttcgaataga ctttcatctt cggagccata tcctagtgca gtaatataat tggataatgc
     2461 atcgatccat acatacacca catgtttcgg atcactcttg actggtactc cccaagtaaa
     2521 agttgttcgt gaaactgcaa gatcctctaa tcctggtttg ataaagttgt tgatcatttc
     2581 gtttttacga gattctggct ggatgaattc gggatgttct tcatagtatt ccaatagacg
     2641 gtctgcgtat ttgctcatgc ggaagaagta tgattcttct tttaccaatt ctacttcgtg
     2701 tccacttggc gctttaccac cgatcacgtt accttcgtca tctttgtaca cttctgcaag
     2761 ttgtgtttct gtaaagaatt cttcgtcaga aacagagtac cagccttcat attcacctaa
     2821 gtaaatatct ccctgttcta ataaacgatc aaatattttt tgaacagctg ctttatggta
     2881 atcatctgtc gtgcgaatga atttatcata actgatatca agcgttttcc ataatttctt
     2941 gacatcagcc gccattttat ctacatattc ttgcggtttt acacctagtt cttctgcttt
     3001 tgtttcgatt ttttgaccat gttcatccac tccagtgaga taaaacacat caaagcccat
     3061 caaccgttta taacgagcaa ttgcgtcaca tgcaatcgta gtatacgagt tgccgatatg
     3121 aagtttgccg cttggataat agattggcgt agtgatataa aaagtttcct tttctgccac
     3181 ggggctcttc ctcctttatc gcatgatgcg aattcgtttc cttgctagta taccataaaa
     3241 aaagcatgct gttagctttt tttcaaaatg aaaaagactg gataaacgac ttctatctac
     3301 gttcaatcta tcctttaaaa ggatgaatag gttgaataaa tgcgggtttt tgttttgttt
     3361 catatctact aaaaaacggc tttttatttc tcccctcaca cgtggtataa taaagactat
     3421 ctatcttagt aagggactgg aacaatcatg cagaagaaac atcttttttt tacacttagt
     3481 attgcttttc tttcattagc acatttaata ttcagctatt tttatattcg tatgtatggt
     3541 tattttaacc tccatgggca tttgaacagt tttatgacag cagcttggat tttgcgtttt
     3601 atcattgacg tatatatcgt tatttgtggc ttctttgcca ttcgtgaaga gcggtataaa
     3661 gtattgccgt tttatctgct tttcttttta ttcaatctca tacttccatt tatttttcat
     3721 atttaaatga taacctagat ttttcaagta aatccttggt tttttttcag agtctgctac
     3781 aataaggtaa aaagtacttt tactgtcaat ggaggtggtc agatgaaccg ttttgcttct
     3841 aaagaagaag aaaaattata ttatgaacat aaagcaagtg aagaagaatt tcttaactgg
     3901 tatcaaaaac aggaacgtcc agaatatgac aagccctctt tgactgtcga tatcgtactg
     3961 atgtgctata acaaagaaga agatcagctg aaagttttgt tgatccaaag aaaagggcat
     4021 ccttatcgaa attcatgggc attgcctggt ggctttgtcc aaaaggatga gtcaactgga
     4081 gaaagcgtct taagagaaac aaaggaagaa actggtgtgg ttatttcaaa agaaaatatt
     4141 gagcaactgc atacattcag tacgcctaat cgagatccgc gaggctgggt cgtcacagtc
     4201 agctacttag cctttattgg agaagaaccg ctaatcgccg gagatgatgc caaggaagta
     4261 cgctggttca cactagaacg tcatagtaac aagatccatt tatctagcgg agaagtggct
     4321 atctcgcttg atttagtcac tggagagtct tctggcaaag atactttggc ttttgaccat
     4381 agtcaaatca tcttgaaagc attcaaccgt gtagtcaata aaatggaaca cgagccgcaa
     4441 gttctacaag ttttaggtaa agatttcacg attacggaag ctcgaaaagt atttgctaaa
     4501 ttcttaggga tcgattataa aacgatcgat cattccaatt ttaagaaagc tatgctccaa
     4561 tatttcgatg agatcggtga acgtcctgta ggaatcggcc gtccttcaaa aatctatcag
     4621 ctcaagccgg ggcaccatga ataaggttgt gagcaaaaga ttcagaccta atcattcaag
     4681 aaaaaacgca aaaacaactc tttgtgagtt acgttttttg tttcagaatt ttgatgtatt
     4741 cgtaaacttt ctgaataaaa aattaacaat agcagaaaaa gctcgtacga actttttctg
     4801 ctattgtttt attggtttgt cataattagt gagcttttct tcaaaagaaa aattcctaga
     4861 aacaaaagtc attaagactt tttattaaca aggctaaccc taacgctcct atcagtttca
     4921 ttcatttttt atcccttgat aaccgaatgt ctgcccattt tttctactac ttggctcttt
     4981 cttacggaat tgctttcttc tgctccgtcg gtattgatca aaagaaattt tttattatac
     5041 cattgggact tataacgcaa ttttgttatt aatttatgcc agtcgtgaat agaaaaatca
     5101 gcatgtgcct cttcttttgg aaaagattct acaaataagt agtcatattg tttgaccaat
     5161 ttcccagata attgttcaag ataatcttct cgctggttct tttgtttcag ataaagatcc
     5221 attacttttc tcttttgttt ctgataattg cttgcctgat ctaacctgat ctttctatgg
     5281 tgagcggagc ggactttcac attcaatttc cgctgtgcat gttgcagctt ttcttctgtc
     5341 tggtgaaatt ttggtaaagt tacttctaga ggtcgagatg tttggatcaa tgtcttcgga
     5401 tgataggcga tcccgatcca tttagctaaa gattcttttt ttactggcga aacatcacaa
     5461 agtagtgcga cataaaattc ttcattatat cgagcagaaa tcgtagcgga gcgaatcgat
     5521 ccttcaactg gccgatgctg atttatcttg attagttctt tttgttttgg taactttaag
     5581 tagccatttt ccagatagat cgtacgggtt tggttgttgg tcgtataaga ttgccacata
     5641 ctttttttcg ttttaagctt tgggaagctg gcacgtccct tgaaataatt gcgaaaagcc
     5701 cgatccaaat taagctgtgc atttgctaaa gcaagactgt ctacttcttt taagaatggg
     5761 tattcttttt ttaatgtagc gggtgtgagc gaaaaattgg ctactcctgt ttgttgccaa
     5821 cttttctgcc gctttttcag caaatggtta taggtaaatc gagcacatcc aaaagtacga
     5881 atcaattgct gtttttgttc ttctgtcgga tacatacgga atttaaaagc ttttagcatc
     5941 tatgtccctc ctagttagca accagaagag attccttttg tcgttttagt ttttttgcgt
     6001 aatatgggac ttcctgctca gttgcataga caaaatgttc tggtgcgatt tcacgaagtt
     6061 gacgtacttg tccgtcatct ggttcaggtt gatatttgat tcttctgcgt tttctctcat
     6121 ggtctggatc tggtagagga atagcagata acaaactttc tgtataagga tgaacaccgt
     6181 gattatagat ttcatcactt gttcccatct ctaataacaa gccgttatgc attacaccga
     6241 ttcggtcact aatatgttta accatcgaca aatcatgtgc aataaataga taggtcaatt
     6301 gatgttcttt ttggagatct tgcaacaaat tgaccacttg ggcttggatc gacacatcta
     6361 gtgcagaaat tggttcatca caaatgataa aacgcggttt tactgccaat gctcgtgcaa
     6421 taccaatacg ctggcgttgc ccacctgaaa attcatgagg ataacgagta ccgtggcttg
     6481 gattcaatcc aacagttctc aatagatcat cgactttttt tgcacgctct tcagatgatt
     6541 tagctagtcc attaacatca attccttcgg caatgatatc tctgactttc atacgaggat
     6601 tcaacgaagc gtatggatct tgaaagatca tctgaacatc atgccggaat ttcgtcaaac
     6661 cttttttatc tttgatcttc gttatatctt gaccgtcgaa taatatctca ccatcagtag
     6721 gttcattcaa gcgaatgatc gtacggccag tagtggattt tccgcttcca gattctccaa
     6781 ctaaaccgaa tgtttctcct tcataaatgt gaaagctgat gtcatttaca gcatgtactt
     6841 cattcttttt tcctacattg aaatactgtt tcaatccttt aacttcaagt agtttatttg
     6901 gcatcagcaa ttcctccttg accgtaagta tgtttttgtt tctctttaaa gatagcccag
     6961 cgacgttgga tttcttctgg cggtgtcact ttgggtgcct gcggagcaag tagccaagtt
     7021 gctgccttgt gtgtttttga tagttcaaaa aatggtggtt cttgttctgc atcgattttc
     7081 atcgcgaatt catttcttgg atagaatgcg tctccttttg gcgggttcaa taaatccggt
     7141 ggagaaccag gaatagcata caatcgatcg ttcgcactat ctaatgtcgg cattgaacca
     7201 agcagtcccc aagtataggg atgttgcggg ttgtagaaaa tttcttccga tgttccaact
     7261 tcaacgattc ttcctccata catgacagcg acacgatcag cgacattcgc tactacacca
     7321 aggtcatgag tgataaagat aattgatgta tcgatttttg tctgaatttc tttcagcagc
     7381 tccaaaatct gtgcttgaat agtcacatca agcgctgttg taggttcatc agcaatcagg
     7441 atttccggat aacataccag tgcaatcgcg atcacgatcc gttgacgctg acctcctgag
     7501 aactgatgcg gataactgtt gatacgtttc tcagcttcgg gtattccaac taatttaagc
     7561 aattctaatg cagctttttg tccctctttt tttgaaactt tattatgctt tctaagggac
     7621 tcagcgactt gcttcccaat gggcatcgtc ggatctaaag aggtcattgg atcttggaaa
     7681 atcatcgcta tttctttacc acggattttt tgcatctctc tttctgattt tccaacgata
     7741 tcgttacctt taaagaggat ttgaccagca tctatattgg cgttgcttga cagcaagcgc
     7801 atgatgcttc tggtcgtcac tgattttcca cttccagatt ccccaacgat cgccaatgtt
     7861 tctcctttat tcaaatgaaa attgacaccg cgaatcgctc gtacctttcc agcatacgta
     7921 tcaaaagaga tttctaagtc tttcacttct aaaattttcg tcatcgtctt cactctttca
     7981 ttttaggatc aaaggcatca cgcaatccat cagcaagtaa gttaaagcca atcatgataa
     8041 ccgataaagt tactgccgga atccataata gataaggcag atacaggaat gtcttgtacc
     8101 cttcgctcaa catcgttcct aatgaagctg acggaggcgt aagacctaaa ccgataaagc
     8161 ttaaaaaggc ttcgaagaaa atagcagatg gaatactaaa catcatttga ataataatga
     8221 cactagagat attcggcaaa atatgtttga atgcaatttt cattcttgat tcacctaatg
     8281 tcaatccagc taaaacatat tcttgatctt ttaatttcat tgtttgtgca cgaacgatac
     8341 gtgccattgg tatccagttt gtgatggcca tagcagccac gattgaaaaa attcctggtt
     8401 caaaaacgac caacatcaaa atcatgacaa cgaggtttgg aattcctgat aagatttcca
     8461 aaaaacgttg catcacgtta tcgacacgtc cgcctaacaa accagaaacc aacccgtaag
     8521 ctacaccaat cgttacatct agtaatgcag caataaatgc gatcagcaac gagattctca
     8581 ttcccataaa taagcggctt aatacatcac gtcccaaacc gtctgttcct aaaaagaaat
     8641 ttacgtttga agggacgttt gcttgagcat atttatcaac aagttcacca ccaaccattg
     8701 cttttccatt caatccatcg atattgattc ccggaatacg aggaggcaaa ttgatataag
     8761 ccacgttttg ggcagttgga tcatgaggag aaacaaagat tgtgatcaca gagatcaaaa
     8821 tgatgaccaa tagtaaaacc attgaaataa cggcagcttt gtttttcttt aaacggcgcc
     8881 atgaatcttg gataaaattc aaagaaggtg cagtaatctg ttcacgttct tcttcggtat
     8941 ttttttgtaa tggaagaaat tcatccgcag ggatatctgc aactgtttca taacgctttg
     9001 gtatcatttc catttatcct cggctccctt ctgaaacacg aattcttgga tcaactagac
     9061 cgtacaatag atccacgatc aaaataacaa aaatcagcat aaatgagtaa aggatcgtta
     9121 cagccatgat cgtaggataa tcatttgttg tgattgactt aacgaattgt tccccgatac
     9181 caggaatagc aaagatattt tctaccacca gtgatccggt catcaatgct acagctaacg
     9241 gtcctaataa agtcatcaat ggaatcaaac tatttcgaag accatgttta aaggcgattt
     9301 cccaacggct caatcctttt gcttttgcta attccacata atcactatgc agtacttcca
     9361 ccatttccgt acggataaac cgagcagagt cagcaagcgg cgacatggcc aacgcaattg
     9421 ttggtaagat tgtgtacaca aagccatccc attttgcgat tggcaaaact tgtaatttga
     9481 ttgcaaagat atattgtaat aaaacagcaa atacaaagtt tggaatggaa cgacctaata
     9541 tcgctacgag tgtagaagat gtatctgccc atgtattttg cttcatggca gagatcgttc
     9601 ctaaaatcgt ccctaggact gttccaaaga taattgcctg taaccctaac tgcaaggatg
     9661 gtccgattct tccagctaac aaatgtgcga ctggctggtt tttaaattgg aaggaaattc
     9721 caaaatctcc ttgaagcaaa ttagaaagat aaatcccata ttgtacgata actggtttat
     9781 ccaaaccgac ttgtttattt agcatctcga ttgtttcagg actcaaacgt tcctgattcg
     9841 tatatggcgt gcccggcagc aattgcatca agaaaaaggt gattgttgca attaaccaaa
     9901 gcgtgatgat catgaaaaag acacgtttta atacatattt gatataacta ttcactaatg
     9961 tttcccccta gcttttgact tctgacagat gtttcgctac attattagat aatgaaccaa
    10021 aaataaagga gcaatcaaat gaaaaaaaaa tctatcccct acgcagtagc ttttctactg
    10081 attctggtta ttttaataaa aaatctcatc aaccattcct ttacactcat tcaattatct
    10141 aatgatctat tcttatggtc attgccgttt ctgattatcg gcggtttttt atgggtcttt
    10201 tcttcgggtt tcttcgatca ttttcagcga tcggtccatc ttgcacgaac aaggaaccga
    10261 aagaaaaaac cagaattttc ctcgctctcg tctgccagct acggtatgta cagcttttgg
    10321 ctgatcattg ccggaatact aattgctctt tctgctattt tcatgctttt ttctttgtta
    10381 ggataagtga atatgagaac gaggttgcga catttgccgc agcctcgttt tttctcatgc
    10441 tcacggtatt aacgattatt cttcaacgta tgtccatttg taatcccatg aagcgcctgc
    10501 cgcatggtga acgatacctt tgattttttc attcactagg tgaccttctg ctttttggta
    10561 caccggaacg acacccatat catcagagat gatcttgatt gctttttgga aatcatccca
    10621 acgggcttgt tcatcgccag cgtctgttga tgaagctgct ttaactgctt cgtcatattc
    10681 tgcattgctc cagcgaccac ggttgtatga attacctgtg acaaacaaat cagtaaagct
    10741 gcttgggtct gcatagtcag cagcccatcc gcctagtact gcgtcaaaat cacccgaagt
    10801 tgagcggtca agacgtacag agaatggaac aggtgttggt ttgactgtta cgccatccaa
    10861 gttttcttgg attgagtttt gaacatattc gattactttt ttcgatgaat catcatcaga
    10921 agccatcaaa tcaaattcta aagaatcaac gcccaattct tttttcgctt tttcccaata
    10981 ttcttttgct ttatctttgt cataagaaac taattttcca gcttcttttg caaagtcttc
    11041 gttcgttgtt ggacttttta ccatatctga tgggatcaat cctgtagaga caactgatcc
    11101 gtcacctagt acttgtttta ctaaagcttc acgatcgata gaataagaaa tagcttttct
    11161 taggttcaca ttgttgaatg gtgaattctt ttcacgttga ttgaattcga tatagcttgt
    11221 ccgcgcttct ttaacagaag tataagctgg atcgtttgcg ttttgctgtg ctaactcacc
    11281 agacaagatc acgtcatctg cttggccatc tttgaataag ttcaatgcag tagaagattc
    11341 ttttactaca ctgactttga tttcagaaag ttttactgta tctttgtccc agtaatgatc
    11401 atttttctta taagtccatt ctgtatctgt tcctggtcca tcaaaatctt ctaatgtgaa
    11461 tggtccatta taaacagctt tatcacttgc agatgcatag gcatccccat tttcttccac
    11521 tacggattgt ttttgtggga aaaatgatgg gaaagcaagc agatattgga aatatggtgt
    11581 ttgtttcgtc aatttgattt ctaattcata gtcaccaact gctttgatcc ctagttctga
    11641 tttatctttc tttccagctg taatgtcttc tgcattttca actggcgcat acaggtaagc
    11701 atattcagaa gctgtagctg ggtctactgt tcgttgccag ccataaacgt aatccgctgc
    11761 tgtaacagga tcaccatttg accattttgc gtcttctcgt aatttgattt tgtaagttaa
    11821 tccgtcttca ctgacttctg ccaactcgct agctcctgcc ggctgtggtt tgctgtcttc
    11881 atcaagacgg taaattcctt cataaacgtt attcaatgct gtaaaactga ttgtatctgt
    11941 tgcaactgat aagtcagctg taggcatttc ttgaggcacg actaagttaa atacgcctcc
    12001 tccatctgct gaaccgcttt ttgctgaact actgcttgtg gctgaactgc cgccataaca
    12061 tccagcaagt acaaggccgc ataccgcaac caaaccaaat gtaaatttct ttttcatttt
    12121 acgtacccct ttattggaat tttcagaata attaatattt tctgaag
//
LOCUS       DACEJR010000084        12145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEJR010000084 DACEJR010000000
VERSION     DACEJR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.085"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..578)
                     /gene="frr"
                     /locus_tag="IW651_002433"
     CDS             complement(21..578)
                     /gene="frr"
                     /locus_tag="IW651_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0815137.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(581..1303)
                     /locus_tag="IW651_002434"
     CDS             complement(581..1303)
                     /locus_tag="IW651_002434"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0815138.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1439..2320)
                     /locus_tag="IW651_002435"
     CDS             complement(1439..2320)
                     /locus_tag="IW651_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0815139.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="IW651_002436"
     CDS             complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="IW651_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0815140.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3560..4039
                     /locus_tag="IW651_002437"
     CDS             3560..4039
                     /locus_tag="IW651_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0815141.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4256..5347
                     /gene="phnW"
                     /locus_tag="IW651_002438"
     CDS             4256..5347
                     /gene="phnW"
                     /locus_tag="IW651_002438"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0815142.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5340..5468
                     /locus_tag="IW651_002439"
     CDS             5340..5468
                     /locus_tag="IW651_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0815143.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5472..6017
                     /locus_tag="IW651_002440"
     CDS             5472..6017
                     /locus_tag="IW651_002440"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0815144.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6470..8161)
                     /locus_tag="IW651_002441"
     CDS             complement(6470..8161)
                     /locus_tag="IW651_002441"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0815145.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8581..9528)
                     /gene="arcC"
                     /locus_tag="IW651_002442"
     CDS             complement(8581..9528)
                     /gene="arcC"
                     /locus_tag="IW651_002442"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0815146.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9643..10662)
                     /gene="argF"
                     /locus_tag="IW651_002443"
     CDS             complement(9643..10662)
                     /gene="argF"
                     /locus_tag="IW651_002443"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0815147.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10753..11982)
                     /gene="arcA"
                     /locus_tag="IW651_002444"
     CDS             complement(10753..11982)
                     /gene="arcA"
                     /locus_tag="IW651_002444"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0815148.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3314 a         2523 c         2194 g         4114 t
ORIGIN      
        1 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
       61 ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      121 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      181 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      241 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      301 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      361 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      421 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      481 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      541 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      601 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      661 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      721 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      781 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      841 cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      901 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
      961 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1021 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1081 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1141 cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1201 tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc
     1261 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1321 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1381 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1441 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1501 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1561 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1621 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1681 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1741 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1801 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1861 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1921 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     1981 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2041 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2101 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2161 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2221 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2281 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2341 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2401 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2461 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2521 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2581 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2641 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2701 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2761 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2821 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2881 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     2941 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3001 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3061 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3121 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3181 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3241 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3301 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3361 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3421 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3481 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3541 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3601 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3661 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3721 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3781 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3841 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3901 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     3961 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4021 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4081 tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt
     4141 attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4201 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4261 taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4321 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4381 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag
     4441 ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4501 ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4561 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4621 gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4681 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat
     4741 agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga
     4801 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4861 tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct
     4921 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     4981 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5041 aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat
     5101 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5161 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt
     5221 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5281 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5341 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5401 ctgtccacgc ttttagaaat gcttttctag aaaaaggaac ccaactgact gacaaagaaa
     5461 tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5521 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5581 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5641 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5701 cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt
     5761 attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt
     5821 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5881 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     5941 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6001 ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat
     6061 ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga
     6121 atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac
     6181 agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca
     6241 agcttttgaa caccgtttat tcgaaaaaaa aagggagctg tgacacgact tttgtcacag
     6301 tccccttatt tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa cggtgttcat
     6361 tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt
     6421 tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct tacatcttat
     6481 ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc actgcataaa
     6541 ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta tgagcatagt
     6601 atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac ggttcgtatt
     6661 tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc acttcccagc
     6721 tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct gctttttcca
     6781 acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct ccttcaaatc
     6841 gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga
     6901 aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc aaatcagggt
     6961 ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact tcttctaata
     7021 agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg
     7081 gaatatggtg catgtcgtca gaccagtcaa agcccatttc cttcaagaca gctttcaact
     7141 gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg aagtcgtaag
     7201 tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg
     7261 acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc tctgcacctt
     7321 tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc atcttgtcat
     7381 tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca tagattttat
     7441 tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc tctttatcgc
     7501 cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt tctgtttcag
     7561 ctacttcatg gaattggacg tataaacgaa gcaattcatt gattggttct gctttaacag
     7621 cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt gttccccagt
     7681 cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg aaaccgatcg
     7741 aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca atattaggag
     7801 aagacatgtc gattggtact gttccttgat tcccgatgtt gctatcccca taatgttctt
     7861 tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc atgaaaaagt
     7921 tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt ttttctgcta
     7981 aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa
     8041 atgcaacatc tccgtgttca gctgattttg gattttctag taattgctcg acttgttcca
     8101 gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct ttattattca
     8161 tctttattcc tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc
     8221 atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca attgtcctaa
     8281 aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca gaattctcca
     8341 agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc gctagccaag
     8401 aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt cttgaattat
     8461 atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa aaaatccaga tggcctaaag
     8521 gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa
     8581 ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa
     8641 agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcttctac
     8701 tttcggaagc atgcttcctg gagcgaattg cttttcatca atgtattgtt tcatttctga
     8761 aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc
     8821 tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc
     8881 aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg
     8941 aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac
     9001 acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc
     9061 agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc
     9121 cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac
     9181 aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc
     9241 gcccatggcg ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc
     9301 taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc
     9361 gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac
     9421 taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga
     9481 caagatggca ttgcccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct
     9541 ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc
     9601 tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa
     9661 caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg
     9721 gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat
     9781 gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa
     9841 gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat
     9901 ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac
     9961 ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat
    10021 catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc
    10081 aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac
    10141 taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc
    10201 tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca
    10261 ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat
    10321 tttttgactg aatccacgga attcgattcc gtcaaacata cgtcctagta ctttagctgt
    10381 atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg
    10441 tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc
    10501 aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa
    10561 gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt
    10621 ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta
    10681 gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt aagactgccg
    10741 tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc
    10801 accacgacct cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg
    10861 aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc
    10921 caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc
    10981 agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt
    11041 atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt
    11101 accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa
    11161 tacggtatcc aaatgcatca ttgcacggtt gttcggaatt ttgattgcca acactttttc
    11221 aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt
    11281 tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc
    11341 gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg
    11401 atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat
    11461 cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt
    11521 tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcat atagactgct
    11581 agaacgtact tcgatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg
    11641 cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg
    11701 agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc
    11761 tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa
    11821 gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc
    11881 cataatatct ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta aaactgtctt
    11941 taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct
    12001 ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt
    12061 caaaatgaga cattcctcac attaatattc gataattaaa taattaaacg tttttctatt
    12121 tttaagcgta atttattcat ttttt
//
LOCUS       DACEJR010000085        12058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEJR010000085 DACEJR010000000
VERSION     DACEJR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.086"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            558..1529
                     /locus_tag="IW651_002445"
     CDS             558..1529
                     /locus_tag="IW651_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0815149.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1607..1843)
                     /locus_tag="IW651_002446"
     CDS             complement(1607..1843)
                     /locus_tag="IW651_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0815150.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1812..1943)
                     /locus_tag="IW651_002447"
     CDS             complement(1812..1943)
                     /locus_tag="IW651_002447"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0815151.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2019..2402)
                     /locus_tag="IW651_002448"
     CDS             complement(2019..2402)
                     /locus_tag="IW651_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0815152.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2435..3220)
                     /locus_tag="IW651_002449"
     CDS             complement(2435..3220)
                     /locus_tag="IW651_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0815153.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3398..4498
                     /locus_tag="IW651_002450"
     CDS             3398..4498
                     /locus_tag="IW651_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0815154.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4488..5312
                     /locus_tag="IW651_002451"
     CDS             4488..5312
                     /locus_tag="IW651_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0815155.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5318..6124
                     /locus_tag="IW651_002452"
     CDS             5318..6124
                     /locus_tag="IW651_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0815156.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6121..6951
                     /locus_tag="IW651_002453"
     CDS             6121..6951
                     /locus_tag="IW651_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815157.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6958..7989
                     /locus_tag="IW651_002454"
     CDS             6958..7989
                     /locus_tag="IW651_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0815158.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8096..9369)
                     /locus_tag="IW651_002455"
                     /pseudo
     CDS             complement(8096..9369)
                     /locus_tag="IW651_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9596..9781
                     /locus_tag="IW651_002456"
     CDS             9596..9781
                     /locus_tag="IW651_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815159.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9905..10621
                     /locus_tag="IW651_002457"
     CDS             9905..10621
                     /locus_tag="IW651_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0815160.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10523..11043)
                     /locus_tag="IW651_002458"
                     /pseudo
     CDS             complement(10523..11043)
                     /locus_tag="IW651_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11331..11984
                     /locus_tag="IW651_002459"
     CDS             11331..11984
                     /locus_tag="IW651_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0815161.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3492 a         2211 c         2737 g         3618 t
ORIGIN      
        1 aacgtgattt tttgctgtaa tgacaaggtt ttagctgttt cagataccat gtatccaaaa
       61 atagtttatt gagaaaatag gagagtattt gatactaact agtaggaaaa aatgagataa
      121 aaagtaaatg gaatcattta aggtaatctg aaataaaaaa tatacagccc tgtggtactt
      181 tacctacaag gctgtatata ttttttactc tcagcgtttt tgactccgtg tctgactccg
      241 ttttattgat tttgaatgtt attcaatgac catttaacag gtcaaatttg atgtttttga
      301 ttccgtatga cgccgtttga tatctagtga tatagtgatt tgagtctaca tcgaataagg
      361 gaataaacca ataaaaaatg atttagaata ttaaaataac aaaaaagagg actacctgta
      421 ttagtcctct tttttgacca gattttgacc agtctatttt acatcctagt atatttatat
      481 gagctaattt tatgattaaa tggtaagttt ttccttgtgt gttttagttt aaactagtgt
      541 gtaattcaaa aatctatatg gaaggaacag tattaagaat agaacagggt tctctacatg
      601 atggtgctgg tttacggacc gttgtttatt taaagggctg cccattacgc tgtgcctggt
      661 gttcgattcc cgaatcacaa agcaagcaga ttgaaaaagg atttggccag accatgaccg
      721 ctgaagaggt aatggatgag attgagaagg atgctgtttt ttactttcac tcggatggcg
      781 gagtgaccat tagcggaggc gaggcactgg ttcaggcgga ttttgctaaa gaaattttgc
      841 aaaaatcgaa atacattgga atcaataccg tactggaaac cagcttttgt ggtgcttata
      901 atgaaatcca aaaggtagct ccctatgtgg atacattgtt tgtggatgta aaaatgttta
      961 ccagcaagct ccacaaacaa tggaccggct tagataacca acaaattttg aagaatattc
     1021 gtcgcttttt aattgagtat cctaactgcg aagtgcgaat tcgagttcct gtggttccag
     1081 gtatcaatat gaacctgacc gagctgctga cgattgcttg ttttgtggca gatcttgatc
     1141 ggttcgtacc gttagaatta ctaccgtacc actgctatgg tatgcacggt taccaagcat
     1201 tgggactgga gtatccgttg gcagacacgc cagcgccttc acctaaagaa atgtttgcat
     1261 tggcagatca actggctcgc acggttcctc atttgctggt cacagcttta accaaaacat
     1321 ttatttattt attgaatcca agctttatct tatggataaa tgaaagacac tcactagctg
     1381 cagccagctc aagaatagag cttttgctga agtcagtgag tgtcttttta cgatgttttt
     1441 tcgaagttaa tttgtcgaat gaacactggc tcaatcggta catcttcact gtttgtcggc
     1501 tgagctgcga tcgctgctac gatttctaac ccttggtata cctgtccaaa aacagtataa
     1561 cgaccatcca gctccggtag tcctccaagc tccaagtatg cttgctttac attagcaggt
     1621 cagcctgcgc ctttgtgttt tgcacaataa aaaactggtt gccgttggtg tgggggccgt
     1681 ggtcccctaa ggccaaggcg ccattaaaat gataaagtcc gcgatgcact tcatcagcga
     1741 aaaagccctc atagctggaa acagcttcat caccagaacc atccaaagcg ccaccttgaa
     1801 taacaaattc cttaatgaca cgagccgaaa ggtgtgttgt cataaacgcc ttgctcagct
     1861 agggagcgcc agttggctac agccagggga gcttggtcag gaaataagcg gagacgaatt
     1921 tcaccagcat cggtgaccag cacacaaact tcttcctgct gcggtttttc taattgaaag
     1981 agaggaaggt ttgcaactga aacgccttga tcctgccatc aaatatcgaa aatccgccac
     2041 tcgaccttcg cctgatcacc aaggcgagcc attcccaata aaacggtata ggcggtttca
     2101 tccgcttgaa aggtcaattg cagctgtcct cgtaaatcgt tgtagtcatc tggggcggct
     2161 acttgataaa aaagatgaaa ctgcgcatgc aaatattcca aaggctgatc tttaaaggct
     2221 gcaaaataag cctgaacttt ttcccgctgc aagctgtcag ccaccaaaaa attttccagt
     2281 tgttgttttg gaacggctgc taacaaggat cggcaaaccg cctcaatttt caacgcatcc
     2341 caatgtcgcg caattaaata atggatgggt gcctcttgat tgtagtccca caggttgctc
     2401 atttttttca ccactttctt ctattatata ggaatcacaa aatcggcagc ggcgtcaggc
     2461 ggcgttcttt tccttgaatg actagcaact tttctacctg acagtctgcc aacagctgac
     2521 tcattttttt aaagcccagc tgataatctt cggcaacatg agcatccgag cctaaggtaa
     2581 agagctttcc gccgacactt tgatacaaag gaacggcata acggtaaagg tcttcgttgc
     2641 cgtatttcaa gaagctcttg gcatttaact ccatcgccaa ttctcgttca acaacctttt
     2701 tgaaaatcgt tagcagctga gcctcaaaat gctccgcgaa ttcttccgct gtgaaggtga
     2761 agcggcgtaa tccataatca aagtgagtca aaatatctcc ttctggaaaa tccgtcaaga
     2821 cctgcaacat ctgatcaaaa tattccttgg tagtttgaat gggatctttg gttaaaacca
     2881 catcgtccat ataatcaaag cggccatttt gatgaatact taccagcttt aaatcataag
     2941 gatgctgagc gaggtaatcc acgatccgct cttcttgtcc tggaacaacg ccaatctcga
     3001 tacctcgcag aaatttggtg gaataggttt cttttaaaga gtccagtttg tttatatagg
     3061 ctggataatc cggaatgtca tcttttccgg tagatggatt gtttaaatca aagtgatccg
     3121 tagaaacgaa aaactccggc tgataggcta aatagttttc aaatttttcc tctgaatcaa
     3181 aggaaaaata cgtgtggaga tgctgatcat aatactgcat agggagcctc cttttttcta
     3241 ctatcatacc gcaaaagctt tagaaggtta acgggaattc gtcgaaattt acaaaccata
     3301 tacaaccttt acattgttca tgaaaccttt tcaagaaact gattctctca ttacaaagct
     3361 ttgttagaat ggaattatcg aaaggggaga agaatgaatg aaaatgaatg aaaaaattcg
     3421 taacatgact atcggtttat tttgtgcagc aacagtaagt atcctgctga caggctgcgt
     3481 ctcaggtgaa gcaacgaagc aatcggctgc ggcagacgac tctttagata cgattataga
     3541 aaaagccaag gaagaaggag agattgccag cttgggaatg ccggatactt gggcaaattg
     3601 ggtaggcact tggaacgacc ttgaaaaaga atacggctta agtcacgtgg atacggatat
     3661 gtccagtgca gaagaattgg ctaagtttga atcagaagga aaaaatggaa ccgccgatat
     3721 tggtgatgtc ggtatcagct tcggtccgtt ggctgaaagc aaagagttaa ccttacctta
     3781 taagaccaac tattgggagg aaattccgga ttgggcaaag gatgatgatg gggattggtt
     3841 gttaagctat acgggaacca tttcctttat caccgataaa gagaatgtga agcaagcacc
     3901 gactagctgg aaggagctgc aagagggtga ctatcaagtt agcgtggggg atgtgacgat
     3961 tgccaaccaa gcacaatttg ctgtattggc tgcggcgatt gccaacggag gtgacgaaaa
     4021 ggatattcag ccaggtctga attactacaa aaaaatggca gaaaaaggcc gtttgtccac
     4081 cgttgatgcc agcgtagcaa atcttgaaaa aggtgaaatc gatgtggcag ttgtttggga
     4141 cttcaatgga ctaaattatc gtgaccaaat tgactccgag cgttttgagg taactgtgcc
     4201 aacagacggc tccatcatta gcgggtatac aaccttaatc aataaaaatg cccctcatcc
     4261 caatgcggca aagctagcac gggaatatat tttatctgat gaagggcaaa tcaatttggc
     4321 aaagggctat gcgcgaccta ttcgcaaggt agagctgcct aaagaagtag ctgaaaaatt
     4381 actgccggag gatgcttatg aagcggcggt gccagttgaa gacaatgaag cttggaacaa
     4441 gacctctgta gagcttccag agctttggca ggcggaggta ctgacctatg caaagtagac
     4501 ttgacaaact gattgtagtt atactagacg gctgtcgcta cgacacagcc gtcgcccagc
     4561 ttgggttcat gaatcattta gttgagcacc agcaagcaag cttattgaag gtaaaaagtg
     4621 agctgcccag caattcacgt ccgttatacg aagtactgat gaccggcgtt ccagcctatg
     4681 aaaatggcat ctatagtaat taccatgctc agcgatccaa agagtggtcg ctgtttgaat
     4741 tagttaagga agctggcgga aaaacaggag ccgccgctta ccactggtac agcgagttgt
     4801 acaatcaagc cccctataat cgtttggttg atcgcatcca gcatcaggag gagcgactga
     4861 ttcagcatgg catcttttat agtgaggaca cttaccctga ttcgcattta tttgcggatg
     4921 cccattattt agtgaatcag tatcagccag actttttggt cgtacattcg atgaatattg
     4981 atgatgtcgg tcacaaattt accgccgatt ctcatgaata tgcagcagcg gttaatcagg
     5041 cggacagatt attggctatt taccttcctg aatggctgca aaaaggttat caagtcgtgg
     5101 tgactgcgga tcacgggatg gatgcgcacg gactgcatgg cggcagcttg gctgcacatc
     5161 gtgaagttcc tttttatttg atttcaaata aacgccaaaa gctgaaggaa caaactgtac
     5221 cacagctgct ggcggctcca ttgttttgct ggttattagg aattgcaccg tcagagaaaa
     5281 tgcaggacat tcaagaattg atgaggggat gaaagagatg aaaaaacgct tggttctggt
     5341 tcctgcctcc tttatcttct gtgcattttt actgctgccg ctgcttttta tggttctgtg
     5401 cagcttgcac agtgatttaa ctaaccagtg gacattggcc aattacagtg atattttcag
     5461 caacaaatat tacacacagg cctttgttaa cagcgtactg attgctgtca gctccagtgt
     5521 gattgggtta atcgggacgt ttattttatg tttatgctta atgaatctat cgactaaatt
     5581 gcaggagaaa atcacctttg tttccaattt ggcagctaat tttgctggga ttccgctggc
     5641 attctctttt attattttgt tggggaatgt aggtgtgctg aaaatgattt tgccatttct
     5701 agcggacttc aatttgtatt cttggtgggg attgaccatc acgtatacct atttccaaat
     5761 tccgttaggg gtcttgtttt tgtatcccag catgaaggaa gtgaaaaagg aatggctgga
     5821 gtccgcacaa ttgctgggcg cttcatcagc gtatgcttgg ttcaaggtgg ctttgccttt
     5881 tttgcgacca tcgattgcca gcacctttgt catcttattt gctaatggca tggggaccta
     5941 tgaaacagcg tatgccctga ccagcagtaa tgtaaatctt ttaacgattc gaattgcggc
     6001 tttagtgtcg ggagatgtgt tcgccaagcc taatttaggc agtgcgttgg cagtcgcttt
     6061 aggtgtcctg ctggtaacgg caatgttgat tattcaaaag aaaggcaagg tggtgcagac
     6121 gtgaaaaagt taataaaacc gattttgttc tgctttgtcc tgtattactt gctgccgatt
     6181 attggaacga ttttgtatgc cagctccact aaatggtcaa aatcgctttt acctagtgat
     6241 tttaccttgc aatggttcca acagctgctg acggacaggg aatttattgc tgcggtgggt
     6301 cgctcacttt tattagcagg tgtggtcttg gtgacgattc tgttgttgat gattcccacc
     6361 attatttgga ttcatttgta tttccctcgt ttgaatcgtt ggctggaaaa gctgctcctg
     6421 ctgccgtatg ctttaccagg agtgattttg gtaacggctt tgcttcgaac gtatgctgaa
     6481 actggtatcc caatgtttgt agtgttggta ggtgcattgt ttattacggc gttgccgatt
     6541 gtctatctta gtcttaacaa ccagatgcgc ttaattaatc tgaaggaatt agtggatgca
     6601 gcagaaacct tgggcgcccc gatgtctacg attattatcc aggtgctgtt tccaaacatc
     6661 cgtatcggtg tgaccctagt ttctctgatg attttttctt cggtttttgg ggagtacatg
     6721 ctgacgaacc tgttgattgg cggtcgattt cagacggtac gcatctatat gctgcggcgg
     6781 atgaacgaaa acggccattt ggctagcgct gtgatggttt tgtatttgct gtttatggtg
     6841 ggaattgctt taactacctt tatgctgact tatcgacaaa agcaacagct gtcccttaag
     6901 cagacgaggg aagagaagca gtcgaaaaag aagcccttaa aggagttgtg acggaaaatg
     6961 tttttagaaa tacatgatct caatctgttt tttgatgaaa aacagatttt agctattgat
     7021 gaaattcaga ttgaaaaggg tgagctggtt actttactag gtcccagcgg gtgcggaaag
     7081 tctacgttgc tgcgctgtat cacgggattg gaacaaccaa agagcggtga gattgtgctg
     7141 gacaatgaga atattgccga taagcctacg aaggatcgca atatcggctt tgtttttcaa
     7201 cagtatgcat tgtttccaac tatgactgtt tttgaaaatg tagcctttgg tttgaaggta
     7261 aagaagctga gtcctgaggt gattcaggaa aaggtttttg agatgctatc attggtggat
     7321 atgacggagc aggcagataa aaatgtccag tttctttctg gcggtcaacg acaacgggtg
     7381 gcactggctc gctcattggt cactgaaccg aaagtcctgc tgctggatga gccattaagt
     7441 gctttggatg cccgaattag aaaacagctg cagcgggatt tgcgcgctat ccagcaatcg
     7501 ctagggatga cgatgatttt tgtgactcat gaccaagaag aggcgatgcg aatcagtgat
     7561 cggatttttg tgatggaagc tgggcgggtg gctcaggttt ccacctcgaa ggaattgtat
     7621 cagaatcctc agagccgatt tgttgcggaa tttatcggca actataatcg tttcgaatgg
     7681 tatgagctga atcagtatac ccacgagttt tccgctaagg gcaatcgctg catttattat
     7741 ttaagaccgg agctgattca atttgaaccg gttcctcagg gggttaagat tccggttcta
     7801 tggaaggaat catttatttt aggcaacatt caacggtatg tttttcaaac gctggagggc
     7861 aaagaaattt tagtggatcg gttgaacgat cgggaagaga aaattagcga tgccttatac
     7921 attcagctga aagatattct agcaatccct gtggaaattg atactcagca ttttgcacaa
     7981 caaagctagt gtatagtcgg caaacatttc tggctctttg tcaaatagga ctgatagagt
     8041 gatataagaa catctaaggt agaattgatt ttctgcttta gatgtttttt tatgtttaat
     8101 aagtgatcag atgattgatt aagaaaattc tggttctcat cctaacccgt aagctattcg
     8161 tttcaatgct ttgatgtggg tatgcagatt ttctattttg ccatttgagt aaggcaactc
     8221 taaggctaaa cgaatgccgg attcatagtt tataaacgct ttttttatat gatgcagttc
     8281 tcttggaacc gacttgggca ttgtttttac aagctgaaag aagatgtccg cttctttgtc
     8341 ataaaaagct tgaagaattt cttgatagta agcataagct attcgtagtt catcagaagt
     8401 agaaagtaga cgttctacga cttcactttc ggttaaatag gcccaaccaa aattacgcca
     8461 ttttgtccat ttatcataaa caagcgtgtc ctgtcgtttg atcagaagtc gagataaagc
     8521 cttcacttgt cgatagtgtt tggcttgtat tggatcgttt cggtcgaagc ttttcatgac
     8581 tcatagtcaa atatccttaa aactgcgaat gaggtgcttg acgatgtgaa agcgatccac
     8641 aacgagtttg gcatgaggaa agcactcttt tatcagcaaa gcataagagg cgttcatatc
     8701 tgttacgagt atttttacgt ttaaacgtgc acgtcgttca aattgcataa aataagaaat
     8761 gagatctttc ttcttacgtg aaggaagaat atccagtaat ttcccagtat cgccatccat
     8821 acaagaaaaa gaatagggac cgacttgatt tttcaatgaa cgaaattcat ctaccaatga
     8881 acagcgaggc aaccaattta ttttcacgtt tgtcatgggg agcagagaag cgagtgttcg
     8941 cgaaacggtt gaccaagaaa cgccgcaatg tttagaaatc gtcgtcattg cttgtttttc
     9001 agtcaattcc tccaatattt tataatgtac ttgtttggcg ataaaacagt ttttttcaac
     9061 aatgtaggta ttcgccgtaa aataggtgtg acagccacga cagaggtacc gttgctttgc
     9121 caaagcaaga taagtccgtt gattcgcaca cttattgaga aggaccagcg attttttctt
     9181 tccgttctta acgatcagcc catgttgatt cacctgttta cactgaggac agggctgaat
     9241 cgttttaatg agcgtggcag aaacaacgat actgttgatt gaatgtattt ttttcttctc
     9301 gacatgagtg atttgaatgt ttccatcttt gattcctagt aatagtttga taagatgaag
     9361 atcaggcata cagaacgctc ctttgattgt gttgtggtga ttcaattcta acggagcttc
     9421 tgtatgtttt gtttttttat gctcaaaagg actgatagaa tgatttacta tcagtcctag
     9481 aaattatagc gccacatttc tttcaccttt gacttttcag acggcagttt ttatgctgat
     9541 actgtagaga gagcaggaca cagcttttgc ttgttttggg taagggggaa agaagatgac
     9601 caaagcactt atttttgatt ttgatgggac gatgattgac agtgaatgga tgttttacga
     9661 gagtattcat cactatttac tgaagcctat ttactggagc agttcggcta taaaattcct
     9721 tttgcagact atcaacaagg tgttgggacg gcagcagaac tgtcctttta tgaagggata
     9781 agtcaagcgg ttgggagacc agtggatgct gaacattttg agcagcggat cagcagcact
     9841 ggaaaatcgg agtagtctca attctgaact gcgggagcta gactattttt ttgaagcaca
     9901 tccgatgatt aaggaaaaaa ttgattcctt tgtgacgatc gatgatcttc aggtcggcaa
     9961 accagatcca gaaggttatt tgttgtgctt gaaacggctg agggttgaac aggctgttgt
    10021 agttgaggat agtccagtcg gtgcccaagc agcaccgcat atgacagttg atgtacaagc
    10081 gttggatgca gattttttcg cctttagcgg gcataaaatg tgcgggccga caggaatcgg
    10141 tgtattatat ggcaaacgtc aattgttaga acaaatggag ccagtcgaat tcggcggtga
    10201 aatgatagat tttgtcaatc tttacgatag ttcatggaaa gagctgccat ggaaatttga
    10261 agctggtact ccgaacattg caggagcaat cgattacttg aatgagattg gtatggagaa
    10321 tattcatcgt tatgagcaag aactggttgc ctacgtttta cctaaattac aagcgatcgg
    10381 cggtatcacc atttacggac cgcaagatcc tgaacatcat actggcgtgg ttgcttttaa
    10441 tttggatggc atccatcccc gtgatgtggc gactgctctg gatatggaag gtgtcgctgt
    10501 acgttcagga caccattgtg cacaacggtt catcgacgat ttctgtttga ctaattggtc
    10561 gtttaaacag tcgatgataa tgccgtttga gcggctccaa tgtcgtggaa tctttgagta
    10621 agagtttcca atatcgcttg agccgacggt acttcttctg attttcagat tacgtgattg
    10681 attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt gatgatgttc
    10741 atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata gaaaacgggt
    10801 acgttttaat tctaaaatag tcaacttttc tttgactttc aacatctgag tacgtgtcgt
    10861 ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg aaggtgagtc
    10921 atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt caatcagcca
    10981 ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag tatgattatc
    11041 catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac caaaagtgag
    11101 tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc gattttccaa
    11161 caacacaaaa tattatagaa gccttttttc taaaggattt caaggtgtct gtttgcattt
    11221 attaaaaaaa agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagag
    11281 ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat atgaaaatat
    11341 taactgttga agatgataat atgataagag agggaataag tgaatatctt tcagaatttg
    11401 gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt aatagcgata
    11461 taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa gtgttgaaag
    11521 aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt gatgaagaat
    11581 ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca ttttcattgc
    11641 cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat ttagagaagt
    11701 ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata aatggtgaag
    11761 aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat aataatggac
    11821 aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa gaaactcctt
    11881 atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa ttagactgta
    11941 taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat taaagatatt
    12001 tccaaaaatg ttcatacaaa tattttctgt tcttggaata ataattatat tagttcat
//
LOCUS       DACEJR010000086        11739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEJR010000086 DACEJR010000000
VERSION     DACEJR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.087"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1038)
                     /locus_tag="IW651_002460"
     CDS             complement(<1..1038)
                     /locus_tag="IW651_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815162.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVI"
     gene            1509..2816
                     /locus_tag="IW651_002461"
     CDS             1509..2816
                     /locus_tag="IW651_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0815163.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            2935..4134
                     /locus_tag="IW651_002462"
     CDS             2935..4134
                     /locus_tag="IW651_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0815164.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4161..5429)
                     /locus_tag="IW651_002463"
     CDS             complement(4161..5429)
                     /locus_tag="IW651_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0815165.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(5592..6238)
                     /locus_tag="IW651_002464"
                     /pseudo
     CDS             complement(5592..6238)
                     /locus_tag="IW651_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(6646..7149)
                     /locus_tag="IW651_002465"
     CDS             complement(6646..7149)
                     /locus_tag="IW651_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0815166.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(7272..8036)
                     /locus_tag="IW651_002466"
     CDS             complement(7272..8036)
                     /locus_tag="IW651_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0815167.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8144..8419
                     /locus_tag="IW651_002467"
     CDS             8144..8419
                     /locus_tag="IW651_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0815168.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            8857..9807
                     /gene="yidC"
                     /locus_tag="IW651_002468"
     CDS             8857..9807
                     /gene="yidC"
                     /locus_tag="IW651_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0815169.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(9986..10186)
                     /locus_tag="IW651_002469"
     CDS             complement(9986..10186)
                     /locus_tag="IW651_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815170.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            10991..>11739
                     /locus_tag="IW651_002470"
     CDS             10991..>11739
                     /locus_tag="IW651_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0815171.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT         3572 a         2438 c         2093 g         3636 t
ORIGIN      
        1 gatcacatca tctgctaatg cattcaacac ttgacgccga tcatattctg tttcaggatc
       61 ttcaccgaaa ccatcagcta agtttgacgt agcgataaat aaatgtgaca cttttccttc
      121 tttcgcagct ggaacgatat caactaactg atcgatgaat ttcttatcta agagtttatt
      181 gtattctttt gattcttgag ctgcgagttc acttgacagc ttcttcgccc cttcttcgat
      241 atctctaaag gatagctgcg caggagaagc agaaatcgca acgtctgcac aataataggg
      301 atttttagca atttttttga acatcgtctg attttctggt aatgcaaata aataaatcgg
      361 ccattgttca ggattttcga aagaatcctt caagtacgta tcaacagctt ggtaatagtt
      421 gacccaatcg atctctactt cttcatcttt tggatttacg ccatggaaag ttactccatc
      481 tcgggctccg cctcctccat tagaagagaa gttcaaaccg ccacctgtca gttcagatcc
      541 caatgcagta acgacatcaa ccggtgcccc ttctggtaga gatacttctt ttacttgttt
      601 gttgctgacc tcatacaatt tcatggagtc gcggttcaat cccaataaat aataagaata
      661 gttgaactgc ccatttttga taatagccag taaataagga agatcactta catagtattg
      721 atcgtctacc gggatactca aacgatggat ataggtctgg tcagcagtta taatgaccgc
      781 tacactcgta gttgcactac gccaaaacga aggatctcct aacaaatttt caattttttc
      841 ttggaacggg gaccagtcat gttcggaata ttttttctca aaacgtttct ttgcttcttt
      901 cgcaaaattt ttgaatttga tctgatcttt ttccacatct tgatgggcga catgcgtatt
      961 cagcatgatc gtgacgaatg gtcctttagc gccttctgaa gtcaattgtg tcaataattc
     1021 tttatccttt tttgccataa acgtacccct cctaaaaaaa tcattccttt gtttctatct
     1081 aaagtctacc acaacactat ttttccatac aataaaacgt atctaaaaaa ataaaacggt
     1141 ttctacggtt ttaaatttag ctattttttt aagttatctt cataatttcc tcaattattt
     1201 agccccagtc ttcaactatt cttcatgaaa tgctgataaa ctaagaaaaa gaataaaaga
     1261 ggtggatctt atgcatttga ttatgatttt cagtttatta agtattatca gtttatttgc
     1321 tttcagcgct tcccgcttat tagatgcgga aacacagcct attcctgtaa aagtacgtaa
     1381 aaacgaccgc taaacttcca taacgcgcgt tcttatccta aagttttttc agaaacattg
     1441 aaactctctc ccctctctga taaaatttta atttgtagcg aaacaaatta attacaagga
     1501 gatcggatat gtccataaaa aaaacacgtc tagtcctatt attggctagt ctcattcttt
     1561 gccttgcttc ttttctacca gctgttgctg tgcaagctga agatacgttc aaagtaaatg
     1621 caaaagcagc ttttgctgta gatgcgcaaa gcggtaagat tctttatgat caagatggag
     1681 aaaaaaccat ggggatcgct tcaatcacaa aaatcatcgg tctttacatc gtccttgatc
     1741 aagtaaaaga aggaaaactg tcatggggtg ataaagtttc gatttcagat tatgcagaaa
     1801 atttgagtat caccccagat ttatcgaatg ttcctttgca taaagaaaac acctacactg
     1861 tcaaagaatt attcgactct gcaatcatcc agtcagccaa tgcttcaatg gtggccttgg
     1921 cagaaaaaat ctctggcagc gaagctaaat tcactgaacg aatgaaagaa caattgaaag
     1981 attggggaat caaagatgca acaatcgtta atgcatcagg attgaacaac tcttatcttg
     2041 gcgaaaaccg accagaaggc actggagaaa acgacgaaaa tcaaatgtct gctcaagatg
     2101 tggccatcgt tgcccgccat ttgatcttgg atttcccaga aatactggat gtatcaagta
     2161 ctacgaccca aatgtttggc gaaaatacgc aatcaccagt ggaaatggtc aactggaact
     2221 ggatgctccc aggattcgtt aattacaagg aaggcgtgga cggattaaaa actggtacga
     2281 ctgagcttgc aggtgcttgt ttcgttggta cgatcactaa aaacgatcaa cggatcatca
     2341 ctgtcgtatt gaatgcggac ggccatgcgg aaaacccgag tgcccgcttc aatgaaacag
     2401 accgtttgat ggactactgc tatgacaatt ggtcagaaaa agagctcgga aaagctaacg
     2461 caagtattcc tagtctaaaa accattgatg taaaagacgg aaaagaatca acagttcctg
     2521 ttgtgcttaa aagccctgta aaagtatggg tacgaaacga tatggatact ggcaaattaa
     2581 cgatcacgcc aacacttgac aagaagcaag tcactgacaa tgagatccaa gcacctatgg
     2641 ataaaggtac aaaaatcggt accgctacga tcaccttggc agacgataaa ttaggctatt
     2701 tagaagacaa caatgcacca tcaacagaga tcattacgga taaaacagtg gaaaaagcga
     2761 atatattcgt gattggctgg cgacgcgtcg cagaattttt cggcaacttg ttttaattga
     2821 acaataagaa aaacatcttt ccatcggaaa gatgtttttt tgctttttct agtttaaata
     2881 acagcttttt tcatctattt tcgttatact gtataagaaa aaggagttga atttatgaat
     2941 ctaaccagag atctgatctt tttagttctc tatcttattg gggtattctc tatcgtttat
     3001 tggcgaaaac agaagaagga tccttccttg ttcttttacg gcagtacgtg gacatttacg
     3061 gtaatcttaa cttattttgt cgtgttggaa attttttatt cgacctttta ttttttgatt
     3121 ccattgttgt tctttagtat ctttgctttt tcttatttca cagaaaaacg aaggctactg
     3181 aatggtcttt tattcaatgt tttactcatc agtttcggca tctacttgtt tgtcttgctg
     3241 tatgaaacgc aaaatatttt tcttggcgga ctgatcgcct tgatcacgat tccattgcta
     3301 cttgtccttt tatttggtat ttatggattg attgtttttc ttttttggaa cggggtcaca
     3361 gttttacgta gagaatcgca ttcattagct aatcttttaa cgttgatact agccattttt
     3421 cttacactgt ttcttgtttt tgactttttc ttgttaaaat atttgccgca atggataaat
     3481 gctttgtttt tctgcgttcc cctgatcttg atctatctat ttatcgtatt ctacaacttt
     3541 ttgactgtct cattcttgta tcagttcaac cggcctcgat acaatcaaga cttcatcgtt
     3601 gtgttaggcg ctggattgat caatggagaa acagtttctc ctcttctagc caagcgaatc
     3661 aataaggcaa ttgcttttta tcgggctcaa agccgtgcta cattaaatcc gcccatctta
     3721 ttgatgtctg gcgggcaagg agcagacgaa aaagtaccag aggccattgc gatgaaacaa
     3781 tatgccatgg aacaaggaat ccctgaaaga gacattcttg tcgaaaccaa ttcgacaacc
     3841 actttggaaa acatgctcta ttcaaaggaa atcatggatc agcaaatgaa agggccatac
     3901 cgcgcgatct ttagttccaa caattaccac atttttagag ccggtcttta tgcgcggcaa
     3961 gccaaattga aagcaaatgg tattggtgca aaaacagctt tctactactt gcctaatgcg
     4021 tttttaagag aatacctcgc catcttgatg atgaacaaaa aaagacatat gatcatttgc
     4081 ggattgcttt tcgcaggctc cgcattctta tcgctgatta ctttactttt ctaaaaaaac
     4141 aaggtgtgac aaaacatatg tcacaccttg tttttttctt tcattctctt aactattggc
     4201 aaaaagagta tccctttttt gaccgctact tgctgagcag aataaatacc gctgttgcct
     4261 gaacacataa agctgatcaa gcagatcata aagaagtata ctgccgcttg tgaaccgaac
     4321 aattcgatcc ccatcacaaa gcaggcaatc ggtgtatttg tcgcaccaga aaatactccg
     4381 ataaagccca atcctgcaag aaaaggaaca gaaacatgga gcaaaggcgc tagtgctgca
     4441 ccaagcgttg caccaatttc aaacaatggt gtcacttctc ccccttgata tcccgcgcct
     4501 aaagacaaaa ctgtgaagaa taacttccca ataaaatcat agacatgtgc ctgtccatta
     4561 aatgattctt gaagtaacgg aagactcaat ccgagataac gttgtgtttg caaaatcaat
     4621 gctgctgcca caactacagc tccacctatc gtatttcgac aaacaggatg aggaatccat
     4681 tgtgcatatc gcgcttttaa aaagacgatc gaacggctga atacccaacc aatcaaacca
     4741 aaagcaatcc cagcaacaaa aactttgaag aacagcaaca tactccattc aggaatcaca
     4801 cccatttggt aatgtagatg agaaacacca tagctttctg tcatgaaatt tgcaaacagt
     4861 gcagcaaaga agctagggaa gatcgcttct gttcggactt tcccgattgc caacacttct
     4921 aatccaaata atgttccagc taaaggtgtg ccaaatacag aactaaaacc cgcactgatt
     4981 ccactgataa tcaagatttc ccgttctgat ttatccaatc ggaacaaacg tgccgtatta
     5041 tctgctaacg ctccccccat ttggactgct gttccttcac ggccaacaga tcccccaaat
     5101 aaatgggtag ttatggttcc aaacagtgta agtggaataa gccgcaacgg gattttttct
     5161 tctccaccat ttccttgatc gatcactaag ttgtttccac gactagaaag tccgccgtga
     5221 tacgcataga gataagcaaa aagcgcacca ctaactggta gtagacaaag gagccaagga
     5281 tgtgatagtc ggatatctgt cacaactgtc aggctattta agaaaaaagc agacaagctc
     5341 cccatgaata taccgagaat cgctgtgatc accagccact tcagcatata cactaaaatg
     5401 cgtactggac tcggtaattg tttttccata aaaaattcct cctcaataaa aaaacgccta
     5461 ctaaagaaag gggctgtccc cagactttag taggcgtcat tagctatctt tcgttagcat
     5521 ttaaaggcga acaccatcac ctattcactt ccattaaaag atagcatatt tttctgtaaa
     5581 aaacaataaa attatcgttc tttctttaca cgttcagcaa attcatagaa aggagccaat
     5641 ttcgttaact gttgattaca ttggtcacta tcatgacaaa tataattccc ctttttcgta
     5701 tacgtgccat cagaacctgc tttcgttgta gaaagaaaaa gcgaaacatt cgatgttttc
     5761 tgacagattg cacagattcc cttatttatc gttggtgata aagtaccgct aatccctttt
     5821 aatttttcat cttcgtaata gaccatgaat tttttctgcg tacctggatc attccagccg
     5881 atgtacgtat gttcttgcaa gtcgatttca ttccaagctg gttgtttgag ttttttcact
     5941 tttcggaata atttctcgat ttgtggtttg ctaggctgca taaacggctg aacgatttgc
     6001 ttcagctcct ccaatgctct ttctgcttgc gcttttgtca gttttacgtc taaaactgtc
     6061 gttaaaaatg tatcgattct ggcagttcgt gatctaatat gtctcgaatc ttttcagctg
     6121 ctagtgcctg aactgtttgt accgtttttg gatcattgac agatgagtac gcatttaata
     6181 actgttctac ttctcttatt ataaaaaaat attggtatgg ttttattgtt tgttccatga
     6241 ttttaaagtc ctttcttcta accaaagtgc atactttgta gaaaaaagtg atcttctgca
     6301 aagtatgtgt tacctgaaga tcccggactc ttttaaataa gatacgtttt gcatcttatc
     6361 atcaccccct ttaatgctta ttattctatg tacatgattc attcgataaa taaaagtaaa
     6421 ccgtatcttg aacagatagt caactgcaaa cagaaaaaag gactgtgaca aaagtcgtgt
     6481 caaagtccct tgtttcgaat gaatggtgtt caaaagctgg catctgcggt aataagccga
     6541 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6601 cacttctgtc acaacctcaa tatggtctat tattggtttt atctattaaa tcgaggattc
     6661 tttcccaaag taacgttgcc atctcttacg catacttggt gacataggct gagcggcttc
     6721 tttgactgcg cagtatttgt ctacgaatgt tacgatatat ccttctttgt acttaggcgg
     6781 tgcaatcgtt gcgccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaattc
     6841 ggtgatcttt tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtgccttc
     6901 atcaaatttc gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc
     6961 tgttgcttta gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact
     7021 gatggaatgc tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac
     7081 tgcttctgtt tctaaaagat cttctacata agacatatat tcttcgtctt cacgccattt
     7141 ttctgtcata attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct
     7201 aagtatacca ccagtgttcg gattattcta gtcgccttgg ataaaatagc caaagcttaa
     7261 tagttcctta atcataaaga aaaatgataa agaacaatcc ctgccgcaac cccaacattc
     7321 aacgattccg catttccttt catcgggata tatacgttct gatcagtcaa agacaagatt
     7381 tcctgactta ccccctgacc ttcgttcccc atgattattg cgaagttatc tgatttttct
     7441 actttgtcta atgcgtgtgc ttcttcattt aattctgtgc catacactgt aaaccctttg
     7501 tctttcatta aaggaatcac ttctgtcaaa tctttttgca acacaggcaa atgataattg
     7561 cttccctgca tgctgcgcag cacctttgta ctataaatat ctgccgttcc tttgcctaat
     7621 aaaacaccag ccatatcaaa tgcatctgcc gtacggatca tcgtccctac atttccagga
     7681 tcttgtacat tatctaacaa cagccatcct cctgaaaaat ctggcgtgcc ttcagccgtt
     7741 aacgccatca ctgctaagat tccttgtggt gtaggtagat ccgatacagc atccatgacc
     7801 tcatccgaaa ctagaaaagt agaaatctct ttgtttgcgg ccagccagtc tccccattct
     7861 ttcatgcctc gctgtgtcac aaaaagttct tttatttctg catcagacga cgcagcttct
     7921 tcaatcagat ggaacccttc caaaagatat tcttcctttt cttcccgata tttcttttta
     7981 tgtagttttt ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc
     8041 ctcacctccc catgattata tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa
     8101 tcggtatcat taatttataa aataggaaag aaaggtgacg aacatgcgca aagttaaaat
     8161 gaacgtccaa ggacgcgtcc aaggcgtagg atttcgctac atgaccaaga tggtcgctga
     8221 tcaactaggc gtgactggaa cggtaaaaaa cgaagatgac ggctcagtca cgatcgaagc
     8281 aatcggcaat gacgacatca tccagaaatt tattgaagaa gtcaagaaat ctccgagccc
     8341 aagtggacga gtgcagtatg tggatgtcca agaagacccg atgattgagg aacgaaagaa
     8401 atttgatgtg gtgggataag gttgtgaaaa agctggtctg catcaagcat taaggacagg
     8461 ttgtgaaaaa tagctctcca tatttttcaa caggctggac ttaatgccgt agatgcaagc
     8521 ttttgaacac cgtcgataag gttattcttg aggcgctttg cctcaaaaat taaggacagc
     8581 agtgaaaaag ctgatctatc acttgcagtg agttcatttg actccgcttt ttcgggcaga
     8641 aaaaaagttt ttagtttatt aaagtaagaa agaaacactt ggaatcattc tactttttcc
     8701 tagtatttct ttcttgattt tttgtctttt tttcaaaatt tttcgctacg taaaagaacg
     8761 aatatttata tgaaatttct atgaagttga ttgaattcca tgcgcttttt tagtatagtt
     8821 aagtttgtgt aaaaattata gaagaggaag tattctatga ataaatggaa aaactggtta
     8881 ttgggatccg gacttgtcag tatcctgctc tttttatctg gctgtgtgcg gatggattca
     8941 aacggaaacc ctgacacttc gggaatcgtt tatcgagtac tcgtccatcc tatgggccaa
     9001 gcgattacat atctagttga aaatttcaat tggtcttatg gctgggcagt tattgcaatg
     9061 actgtgatcg tccgtatcat catcttgcca ctaggtatta gtcaatcgaa aaaaacaatg
     9121 atccaatctg aaaaaatgca agcgttaaaa ccacaagttg aagcagccca acaaaaattg
     9181 aaagctgcaa caacacgtga agaacaaatg gcagcacaag cagaaatgca gcaagtatac
     9241 cgcgaaaacg gattgagtat gaccggcgga atcggctgtc tgcctctatt gatccaaatg
     9301 ccgatcttct ctgccttgta ctttacggca cgttatacag aaggtatccg ggaatcatct
     9361 ttctacggca tcgatttagg ctcaccaagt atggtacttg ttgccattgc aggggtcgct
     9421 tatctgcttc aaggatacat ctcaacgatc ggtattcctg aagaacagaa aaagacgatg
     9481 cgtacgatgc tgatcgtctc tcctgctatg atcgtcttca tgtcgatcag tgcgccagcc
     9541 ggtgttacac tttactgggt agtcggtggt gtgttcagct gtctgcaaac attcatcaca
     9601 aacgttatga tgaaaccacg tttgaaagca caagtggcag aagagttgaa acaaaaccca
     9661 ccaaaacaag tcgtcactcc aagaattgat gtgacagaat cagctaaacc agctgacagc
     9721 aaacaagcaa ccaaacaaat caagcaaccc aaaaacagta caaacaataa aggccgcaac
     9781 gcaggaaagc agcagaagcg gaagtagata gtaaataaga gtgtaataaa aattcattaa
     9841 cagttttagc aattaaaaat atcgaccaag atttttgaaa aagaaatctg gtcgatattt
     9901 ttatttaagt gaatgtttaa tttgaatcat cgattgatcg ctatctaatt gcagcagcat
     9961 ctttttatga agaaacaact ttttcttatc tatcaacgta gacaggtgtt gctgtttctt
    10021 ctaatagtgt acttaattcc ttaatatttt gttttccttc ttctaacatc caactactcg
    10081 ctttttcttc gccttcggta acaaaaggcg ccactccctt cgcccatgtt gctcgcccac
    10141 ataagacacc attctccata aaggacaacg cggagatacc acccatctaa ataacatcca
    10201 taaacctttc tagtagaaga atagtgatag tatccatccc tttttctact gggaatacaa
    10261 ttacacgatt caaagcttga tacacaacat ttcccttctt ataaaccgct attgtaaccg
    10321 aattgttttg aatcaccttt ttgggataga aactggctcc tttccgcaac ttcttaaatg
    10381 ttggtttctt ttatactttc tttgtagcaa ctacaaattt cttcaaatct ctaaatgttt
    10441 gcataacaac cgcctttctg acttttactc aggatggtat ttaaaaatca gcaaaataag
    10501 gcttctctcc atctttttta tttcctagct gctttaaaga cactcttgct gttttttaat
    10561 ctctatcaag ggggctctga atctattttg atgaacttat cttattaatt tctcaaatga
    10621 gctgtcagac acaactgtat tttttcaatc tcataccgga caactatgcc agcttaaaaa
    10681 aatttcctct ggcaaaactt ctttattgaa aaatgacaat gaaaaaagac cattcttttt
    10741 gttgaatgct cattgtgtaa acatatttcc tgcgactgct cttcgaacca ttctcagtgc
    10801 aatccctaat ttatctatta aatcttcttt tagttcctca tctttcatat tcaaaatttc
    10861 ctttgtatgt tacacaagtt tgttctctaa ataacgcgaa ttgtaaaatt aagttagaaa
    10921 aataaaaagg catttatggt acactcaaat tgtatttccg acgaaagaaa acaaaggagt
    10981 gtaaccataa atgacctaca aacatcttac catagacgaa ctgacaatga tagaatcata
    11041 ttatcttcaa cataataagc cggttgaaat cgctaaccga atgggacgtg ctatacaaac
    11101 tatttataat gtagtcaata agttcaagca aggtaagact gctcttgatt attggcacca
    11161 gtataaagaa aataagaaaa aatgtggtag aaaagtcatt caattacctg ctcatgaagt
    11221 agattacatt aaagagaaag tcactcttgg ttggacgcct gacgtcatta tcgggcgaaa
    11281 agaaaggcct gtttcatgcg gtatgagaac actttatcgt ttattttcta aaggaatatt
    11341 tgatattgac acactaccga tgaaaggtaa aagaaaaccc aatggccatc aggaaaaacg
    11401 gggaaaacaa cagtatcagc gctcaatcca tgatcgacct gataattatc ctgatttcaa
    11461 ttctgagttt ggtcaccttg aaggtgatac gatcgttggc attcatcata aaagtgccgt
    11521 cattacttta gttgaaagat tatctaaagt cattatcacg attaaaccca acggccgtaa
    11581 ggcattagat attgaaactg cccttaatca atggttttct cgcttcccta aaaacttctt
    11641 taaatctatt acgtttgact gtggaaaaga attttctaac tggaaagcca ttagtaacca
    11701 acatgatatt gatatatatt ttgcggaccc tggaacacc
//
LOCUS       DACEJR010000087        11451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEJR010000087 DACEJR010000000
VERSION     DACEJR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.088"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1014
                     /locus_tag="IW651_002471"
     CDS             <1..1014
                     /locus_tag="IW651_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0815172.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IW651_002472"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IW651_002472"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0815173.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IW651_002473"
     CDS             3470..4063
                     /locus_tag="IW651_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0815174.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IW651_002474"
     CDS             4076..4975
                     /locus_tag="IW651_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0815175.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IW651_002475"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IW651_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0815176.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IW651_002476"
     CDS             5817..6077
                     /locus_tag="IW651_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815177.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..6728
                     /locus_tag="IW651_002477"
     CDS             6522..6728
                     /locus_tag="IW651_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0815178.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            6728..6979
                     /locus_tag="IW651_002478"
     CDS             6728..6979
                     /locus_tag="IW651_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815179.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            6995..7411
                     /locus_tag="IW651_002479"
     CDS             6995..7411
                     /locus_tag="IW651_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815180.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            7393..7665
                     /locus_tag="IW651_002480"
     CDS             7393..7665
                     /locus_tag="IW651_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815181.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            7832..8233
                     /gene="tnpA"
                     /locus_tag="IW651_002481"
     CDS             7832..8233
                     /gene="tnpA"
                     /locus_tag="IW651_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0815182.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            8245..9390
                     /gene="tnpB"
                     /locus_tag="IW651_002482"
     CDS             8245..9390
                     /gene="tnpB"
                     /locus_tag="IW651_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0815183.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            9628..9891
                     /locus_tag="IW651_002483"
     CDS             9628..9891
                     /locus_tag="IW651_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0815184.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            9885..10235
                     /locus_tag="IW651_002484"
     CDS             9885..10235
                     /locus_tag="IW651_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0815185.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(10259..10873)
                     /locus_tag="IW651_002485"
     CDS             complement(10259..10873)
                     /locus_tag="IW651_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815186.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            11323..>11451
                     /locus_tag="IW651_002486"
     CDS             11323..>11451
                     /locus_tag="IW651_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ0815187.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSR"
BASE COUNT         4188 a         1875 c         2252 g         3136 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga
     6721 aacgttgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
     6781 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
     6841 gacagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
     6901 tgcaggatat gaactgcatt atcaagaaaa cttggaaact tatgaaaacg aatattggtt
     6961 aaccgaatta gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt
     7021 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa
     7081 tgagacacgt gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat
     7141 gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtat tatcgaacac tggaagaact
     7201 gaaaaagcaa aatgcaaaat ggtttttcca agccttacta tgcttggaag taggcgtcaa
     7261 accaagcaca atcaaacctt ctgaatacca agctttagaa ctaacgtatg cgaaatttat
     7321 cgaaacgaaa aaagctaaga ctgtttcaag tgaatggtta gattattttg aaaatatcaa
     7381 taagtatggc gcatgttaca tcatgaagta atcagacgga ttcaaaaaga ctttagttgt
     7441 atggaagaaa tgaagtactt tgttcaaaat gttgaagaac agttatattt ggaaagcgaa
     7501 acgagtcaaa caaaggtcac agtcagccat aatcgtttag atgtgtataa acgtcgtcct
     7561 gaatcattaa aaaaaggagc ggagcttcta cgactagaaa cagaaaaata cgtcatgaat
     7621 gtttacctag aaaaaagtga gttggtcgaa aattttatgg actagccgag aacacccgtg
     7681 attttaatca tgggatgaat cggattcagt tgtgacctag ctttagctag gttaaacaac
     7741 tgacaaatat aaactaaata atagtgaaat gtcttgcaat aattgatttt gtagaaatac
     7801 atatattaaa ataattgcga ggtgtttttg tatgtcagaa attcattatg gacggggtta
     7861 tgtctattct attcaatatc atcttgtttg gtgtgtaaaa tatcgacatg atgttttgta
     7921 tggtcaggta gatattgatg tcaaacagtt tttgaaacaa attgctgatg ataataatat
     7981 aaaaattata gaaatagaat cagataaaga ccatattcat ttactcattg aatgcacccc
     8041 acaacactat attcctagta ttgttaaagt atttaaaggc gcatccgcta gactgctgtt
     8101 taaaaaacat cctgaactta aacaactcct ttggggtgga catctttgga atccaagcta
     8161 ctttgtagct actgttagcg aaaatacaga agaacaaatt agaaaatata tacaaaatca
     8221 gaaaaagaaa tgaggtgaat ccgtatgcta caacataggg cttataaatt tagaatctat
     8281 cctaatcaag aacaagaaat attaatgact aaaactattg gctgttcaag atttgtctat
     8341 aattattttt tgaatttgtg gaatcatgaa tatacgacta ctggaaaagg attatcttat
     8401 aattcttgtt ctgccatgct tccacaaatg aaaagaaatg cagagacaat ttggttaaaa
     8461 gaagttgaca gtattgcgat tcaatcctca ttgaaaaatc tttctgatgc tttttctcgc
     8521 ttctttaaaa agcaaaataa acgaccacaa tttaaaagta aaaaacatcc tatacaaagt
     8581 tatacaacta aaaatgtgaa taatagtatc gaaattagaa acaatttcgt aaaactacct
     8641 aagttaggat ttgttaaatt tgcgaaaagt caagagccca aagggcgaat cttaaatgct
     8701 acgattcgaa aaaattcgag tggtaaattt tttgtttcca tcctgtgtga agaagaaata
     8761 gttgagtttc ctaaatctaa ttccgctatt ggaatcgacc ttggtataac tgattttgca
     8821 atcctttctg atggtcaaaa gattgataac aataaattca cgtcaaaaat ggcaaaaaaa
     8881 ctcaaacgtg aacaacgtaa gttgtcaaaa cgtgcattac tagctaaaaa gaaaggcgtc
     8941 aatctctttg aagctaaaaa ttatcaaaaa caaaaacgaa aagtagcacg actacatgaa
     9001 aaagtaatga atcaacgcaa tgacttttta aacaagttga gcacagagat tatcaaaaac
     9061 cacgatatta tctgtataga agacttaaat acaaaaggta tgttgcgtaa tcataaacta
     9121 gcaaaaagca tttctgatgt gtcttggtct agttttgtga ctaagttaca atacaaagct
     9181 aactggtacg gtcggaaaat catcaaagta gataaatggt ttccatctag tcaaatctgt
     9241 tcaaattgtg gacacaaaga cggcaagaaa tctctagaaa ttcgagaatg gacttgtcct
     9301 gtttgtcatg ctcatcatga ccgagatatc aatgctagta tcaatatttt gaccgaaggt
     9361 ctaagattac atgtcatggg attagcttag atccaatcat caaaaaccgt aggaactacg
     9421 gggatagctt ggtaaataag agaaaccgct gtgagtaaag aaatacgctt gcaagtatgc
     9481 tctattccca agaatctcgt gactttagtc atgagaggtt caaggattca tggaatgtta
     9541 atctaattta atagaaaagg ataattaagt atatacgttt tttcttatgt atgttatact
     9601 atgtatatac agaaagaggt gtctactatg gaaatgttac ctataaaaaa atggggaaat
     9661 agtaatggcc ttagattgcc aaagtatatt atggaatatc ttggaattca tacagaagac
     9721 aaagtgaaga taatacaaga agaagtaaac ggtcagaagc ggctaattat cgaagcggct
     9781 aatatagaaa atgaattaac aattgaacag ttatttgaaa attacactga tgaaagaaat
     9841 catgtgacga ttcaaaattt aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta
     9901 gacaaggcga tattctatta ttgaatacag cacctcgatc tggacacgaa cagacaggta
     9961 aaagacctta tattgtttta agtcatgata ttattgcgga ttatagtaac gttgttacag
    10021 ttgctccaat ttctagtaca actagaaact atccgctata tgtaaatata aactcagaat
    10081 atgaaatgaa gacaacagga aaagtattat tagatcaact aacgacaatt gattatgaag
    10141 ccagagagtg tatttttttg gaaaaagcgc atgatagttt agttgaggag ttattgatga
    10201 aagttagaac agtttttcaa aaagtaagca aataaaatag aaacagcagc atgatcaact
    10261 attcgtgatt gaccatgctg tcttttttta ttctatatac ggtttgtctc gtaacattgt
    10321 agtcttttgc aatcttagcg atcgcaacac ctttttttag atcctcaacg atgtttttat
    10381 aaacaagacg acgttgggga tcttttgcat tggcactgta tagcttagga cgtcctttat
    10441 aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg acgtcgttta gattcggtac
    10501 gttcttgttc agcgatcatg gccaaaattt gaatgatcaa atctttaatg aatttatcca
    10561 ggagaggatt gccaattgct tcggccatga tcggtagact tgtgatgatc agctgcacat
    10621 ttttcttctt caagtaattg actgaagcga tgatctcgtc gtaatttctt ccaagtcgat
    10681 caatagcttc cacaataaaa atatcttgat ctcggacgaa atccagtgct tcttgaaaaa
    10741 caggtcgatg agtaatcgtt gcgccagatt gtttctcaat gaaaattctc tctgcaccga
    10801 attttttcat ttcttcaatc tgccgttgtt cattttgatc gatggatgag acacgtatgt
    10861 atgcaactct caaaattttt acctactttc tgcaactatt taaaaaacac cctaatcata
    10921 cacgagaaaa ccagattttt caatgtcgcg gtcgtgtcta gttagggtat accttatata
    10981 gacaaaaact aatgatggag gaaatagtat gagaattttt tgtaagtgtc tactgataac
    11041 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    11101 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    11161 tttcaaatta attagtaaag tgatagaata aattgatcat acaaatcaaa agaagaagca
    11221 gggtgatagc atacctatgc ttcttttttt atgtatttag ctatttcttc tttacaatat
    11281 cgaacatatg tttgtataat gtgaaagaat ggagtgttat ttatgatgga ttattcaaaa
    11341 gagccagtaa atgattactt tttaatcgat atgaaaagct tttacgcaag tgtcgaatgt
    11401 atagaaagaa atttagatcc attaacaaca gaacttgttg ttatgagtcg a
//
LOCUS       DACEJR010000088        11394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEJR010000088 DACEJR010000000
VERSION     DACEJR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.090"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(40..1419)
                     /locus_tag="IW651_002487"
     CDS             complement(40..1419)
                     /locus_tag="IW651_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ0815188.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IW651_002488"
     CDS             complement(1440..2987)
                     /locus_tag="IW651_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0815189.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IW651_002489"
     CDS             complement(3236..4015)
                     /locus_tag="IW651_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0815190.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IW651_002490"
     CDS             complement(4090..4548)
                     /locus_tag="IW651_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0815191.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IW651_002491"
     CDS             complement(4560..5819)
                     /locus_tag="IW651_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ0815192.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IW651_002492"
     CDS             complement(5935..6249)
                     /locus_tag="IW651_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0815193.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IW651_002493"
     CDS             complement(6395..7891)
                     /locus_tag="IW651_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0815194.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IW651_002494"
     CDS             complement(8069..8548)
                     /locus_tag="IW651_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ0815195.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IW651_002495"
     CDS             complement(8924..9169)
                     /locus_tag="IW651_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815196.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IW651_002496"
     CDS             9274..10047
                     /locus_tag="IW651_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815197.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IW651_002497"
     CDS             complement(10276..10524)
                     /locus_tag="IW651_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0815198.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IW651_002498"
     CDS             complement(10623..10937)
                     /locus_tag="IW651_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815199.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3543 a         2344 c         1707 g         3800 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt atttggttct ataatatttt gtgttggtgg
    11281 aaaatcgaag gcgattttct actaacactt tttcttcgtt tcaactcaaa aagagactca
    11341 cttttggtaa aatttagtcg accaaaacgt catcctacca aaggagtctc tcaa
//
LOCUS       DACEJR010000089        11340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEJR010000089 DACEJR010000000
VERSION     DACEJR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.091"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..936
                     /locus_tag="IW651_002499"
     CDS             <1..936
                     /locus_tag="IW651_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0815200.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW651_002500"
     CDS             939..3122
                     /locus_tag="IW651_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0815201.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW651_002501"
     CDS             3119..4120
                     /locus_tag="IW651_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0815202.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW651_002502"
     CDS             4135..5049
                     /locus_tag="IW651_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0815203.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IW651_002503"
     CDS             5294..5410
                     /locus_tag="IW651_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0815204.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW651_002504"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW651_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0815205.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW651_002505"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW651_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0815206.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IW651_002506"
     CDS             9380..10642
                     /locus_tag="IW651_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0815207.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11340
                     /locus_tag="IW651_002507"
     CDS             10887..>11340
                     /locus_tag="IW651_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0815208.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3735 a         1897 c         2376 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
//
LOCUS       DACEJR010000090        11276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEJR010000090 DACEJR010000000
VERSION     DACEJR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.092"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            293..1528
                     /locus_tag="IW651_002508"
     CDS             293..1528
                     /locus_tag="IW651_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0815209.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2527..2787)
                     /locus_tag="IW651_002509"
     CDS             complement(2527..2787)
                     /locus_tag="IW651_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0815210.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2814..4115)
                     /locus_tag="IW651_002510"
     CDS             complement(2814..4115)
                     /locus_tag="IW651_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0815211.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4407..4865)
                     /locus_tag="IW651_002511"
     CDS             complement(4407..4865)
                     /locus_tag="IW651_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0815212.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4878..6203)
                     /locus_tag="IW651_002512"
     CDS             complement(4878..6203)
                     /locus_tag="IW651_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0815213.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6220..7632)
                     /locus_tag="IW651_002513"
     CDS             complement(6220..7632)
                     /locus_tag="IW651_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0815214.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7649..8413)
                     /locus_tag="IW651_002514"
     CDS             complement(7649..8413)
                     /locus_tag="IW651_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0815215.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8517..9392)
                     /locus_tag="IW651_002515"
     CDS             complement(8517..9392)
                     /locus_tag="IW651_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0815216.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9647..9731)
                     /locus_tag="IW651_002516"
     tRNA            complement(9647..9731)
                     /locus_tag="IW651_002516"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9695..9697),aa:Leu,seq:caa)
     gene            complement(9749..9820)
                     /locus_tag="IW651_002517"
     tRNA            complement(9749..9820)
                     /locus_tag="IW651_002517"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9786..9788),aa:Gln,seq:ttg)
     gene            complement(9830..9902)
                     /locus_tag="IW651_002518"
     tRNA            complement(9830..9902)
                     /locus_tag="IW651_002518"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9867..9869),aa:His,seq:gtg)
     gene            complement(9964..10048)
                     /locus_tag="IW651_002519"
     tRNA            complement(9964..10048)
                     /locus_tag="IW651_002519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10012..10014),aa:Leu,seq:caa)
     gene            complement(10069..10139)
                     /locus_tag="IW651_002520"
     tRNA            complement(10069..10139)
                     /locus_tag="IW651_002520"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10105..10107),aa:Cys,seq:gca)
     gene            complement(10185..10256)
                     /locus_tag="IW651_002521"
     tRNA            complement(10185..10256)
                     /locus_tag="IW651_002521"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10222..10224),aa:Gln,seq:ttg)
     gene            complement(10268..10340)
                     /locus_tag="IW651_002522"
     tRNA            complement(10268..10340)
                     /locus_tag="IW651_002522"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10305..10307),aa:His,seq:gtg)
     gene            complement(10343..10416)
                     /locus_tag="IW651_002523"
     tRNA            complement(10343..10416)
                     /locus_tag="IW651_002523"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10382..10384),aa:Trp,seq:cca)
     gene            complement(10423..10503)
                     /locus_tag="IW651_002524"
     tRNA            complement(10423..10503)
                     /locus_tag="IW651_002524"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10467..10469),aa:Tyr,seq:gta)
     gene            complement(10505..10580)
                     /locus_tag="IW651_002525"
     tRNA            complement(10505..10580)
                     /locus_tag="IW651_002525"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10545..10547),aa:Phe,seq:gaa)
     gene            complement(10616..10688)
                     /locus_tag="IW651_002526"
     tRNA            complement(10616..10688)
                     /locus_tag="IW651_002526"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10653..10655),aa:Asp,seq:gtc)
     gene            complement(10698..10770)
                     /locus_tag="IW651_002527"
     tRNA            complement(10698..10770)
                     /locus_tag="IW651_002527"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10735..10737),aa:Val,seq:tac)
     gene            complement(10786..10857)
                     /locus_tag="IW651_002528"
     tRNA            complement(10786..10857)
                     /locus_tag="IW651_002528"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10822..10824),aa:Glu,seq:ttc)
     gene            complement(10872..10961)
                     /locus_tag="IW651_002529"
     tRNA            complement(10872..10961)
                     /locus_tag="IW651_002529"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10925..10927),aa:Ser,seq:gga)
     gene            complement(11001..11074)
                     /locus_tag="IW651_002530"
     tRNA            complement(11001..11074)
                     /locus_tag="IW651_002530"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11038..11040),aa:Asn,seq:gtt)
     gene            complement(11084..11199)
                     /gene="rrf"
                     /locus_tag="IW651_002531"
     rRNA            complement(11084..11199)
                     /gene="rrf"
                     /locus_tag="IW651_002531"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3583 a         2428 c         1822 g         3443 t
ORIGIN      
        1 agcacaccta tcaattaata taattatttt atttttttat gttctcatgt ttatttttaa
       61 tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa attgttgcgt
      121 gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga agttgtgaaa
      181 acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc tgtgtttttt
      241 agtatactag taagaaacga agggatagac tcattccaat aaggaggatt tcatgaagac
      301 aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga ttgaggttgc
      361 caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa cttttgctcc
      421 tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta cgaaaaaaca
      481 agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt ttaccaaaga
      541 aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg agcctcgact
      601 gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa agattccttt
      661 agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc atgcgctgat
      721 gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact ggacaaaaaa
      781 gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac aaaaagaaat
      841 gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc tcatcacaac
      901 gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg ttggtccaat
      961 ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac agcgaaagca
     1021 acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc cgcgtatgtt
     1081 acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg cacctattga
     1141 ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag aatatctgtc
     1201 tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg aagggaccgt
     1261 ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat ttgagcaaag
     1321 ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa aaccagtgac
     1381 tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc gtcagcaggc
     1441 acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc aagaaatcga
     1501 aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt taatagagac
     1561 tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa gtaaatagtg
     1621 gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat catccttaaa
     1681 ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta ttcttagtca
     1741 aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa gaaagaccat
     1801 aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag catcgctttg
     1861 tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg atttaccact
     1921 ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa tccgatgcat
     1981 actcctatat aaactgccag caatccccac atattgagta gatcatgtca gaatgttcta
     2041 tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct attcccagca
     2101 caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac atattaccat
     2161 tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct atatttgact
     2221 tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa tttccatttt
     2281 ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta aaaaagggct
     2341 gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa agcttgcatc
     2401 tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa tttaagaagc
     2461 aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca accactttta
     2521 gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa aattgatagc
     2581 aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat gcggtacaat
     2641 gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc gattgatttg
     2701 aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata aaaagataaa
     2761 aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca aacttagaca
     2821 gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt atcataagct
     2881 tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat caagataaca
     2941 acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa tgtccatgtt
     3001 ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact gataatagtc
     3061 aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat cataggagct
     3121 ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa acgaccaacc
     3181 tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac gatcgttgct
     3241 cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat atagggtaat
     3301 tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat gatcagccac
     3361 attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga ccacaacaaa
     3421 tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat attggttaaa
     3481 ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct tcgaatcagc
     3541 caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg ggcaacagca
     3601 ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa acgatatact
     3661 tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc caaagaactc
     3721 ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac aggaacttgt
     3781 aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa tccatatgat
     3841 ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc catcgtcatc
     3901 ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca tccaggaaac
     3961 ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa tgttgaaaca
     4021 accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat tggtgggaca
     4081 agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt tcaataaaat
     4141 atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata taattaagaa
     4201 attttatttc aatatattct cttaaataat caaatttatt tttaagaatc ctgaaaataa
     4261 gttttctaaa tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc aaaatgatta
     4321 aatctagtga ttgaaacata tcatttcaaa aaaattttgt aaattcaccc ataaatatca
     4381 ccctatttaa cttttttcat cgtacactaa aattcaaaac aactatcttt aatctttact
     4441 tcttggtttt gttttaataa atttccttca aaaatgcctg gagaaactac aatgatcacc
     4501 gtatcttttt gtgcttgctc caacttcttc aaatctataa tagctaacag tgtcccttct
     4561 tttacctcct ggttactttc aacccgtatc tgaaacggac ttccttcgag ttcaactgta
     4621 tctaaaccca tatggatcag tatttttctg ccagattcac tttcgatcgt tatggcatgt
     4681 cgggtaggaa atatattatc gattattcct ttaactggcg catggatata tccatcatga
     4741 ttcgttatag cgtatccaac tcctatcatt tttgaagaaa agacctcgtc ttttactgct
     4801 gttaatggaa taatttgacc actagctaca gcttttagaa tgtttcttgt tttaaagaaa
     4861 tgcacttttt atcacctcta ctttaatgct ggccaatatt ttttgtttac tttgatcatt
     4921 tcatctagta ttttttttgc gactctggca tttggtacgg tttgattcaa tgtaaatgct
     4981 tgtaaagctt tttgataaga accttcaaag taagcatcta ctagtaactt ctctactgca
     5041 acttgtgcct ccatcaatcc tttatgaaaa tcactgattg gttttcttag tgtaattggc
     5101 tccacaccag ttgcaccaac atatgccggt atttcaacaa ctgcatctga ccgtagattc
     5161 ggaatcgccc cttgattagg aacgattagc ataaagcgac gacgatcatc attcaacaag
     5221 gaaatagcta tatcgacgat atattgtcca tgctccccaa aattaaagtt caatacttct
     5281 tccacaatac cgcggcgtat tttatcagcc atttctttgg tatttttctc tcgtccatcc
     5341 atgaccatat tagcacgtgt atactctttg tcagtatgct caacgaccat atcaggatat
     5401 aagtaatact ctaagtaatt attcgggata ttacttggaa aatttttcgt gatgactgac
     5461 atcatttgaa aggttttttg ccaagtttta tctcctatat taaaatcggc gacttgcatt
     5521 tcttttgtta ttagtttttc aataatctct ggcataattt ctcgttgcaa ttctttatca
     5581 taaatcgatg tataccagcc aaagtggttt aaaccataat acgtaacgat ccaattctta
     5641 cgatcataac cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat atcacaggca
     5701 ttaatcattt ttacattcgg atatttacga cgaacagctt cagaaacgat cgactctgga
     5761 tttgtataat tcaaaatcca tgctttgggg gcatagtctt gtatatgatc gacaatttcc
     5821 aataaacctt tgattgagcg catcccataa gcaaatcctc cgagtccaca cgtttcttgt
     5881 cctactaatc catgtttaag tggaatcttt tcgtcctttt ctcgcatctc caatcctcca
     5941 acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg atcatccgtt
     6001 gcacgaatgc gaatatttga aaaacctttt cttttcagca taaatttaac aatcaagtac
     6061 atatccatat ttctttcttc atcaatatca tataaacgta tttcagataa tggcaaccgt
     6121 ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat acgtactccc gcctccagct
     6181 atagtgataa tctgtttttt catcatcatc ttttcctttc tattcttcta ataatttatt
     6241 gaattctgcc ataacataag tcacttctaa gccgatgata atttgataat ttttttcagt
     6301 tggtttaatg atcccgctta tccctaattg cttgattcgc tgttcctcga ccatggtggg
     6361 atccactacc tctacccgta ggcgtgtatt acaactatag acatccagga tattggcttt
     6421 tcctccaaac gcttgtagca tttttgttgc catatattca taatttttac ttgctaaccc
     6481 taactctgtt tcttctaagg ttacttcatt gccaaactct gccttagctt cacgtcctaa
     6541 tgtctgaatg tttctcttat tgatcaaata ataaaaagta acgtaataca aggcaaaaaa
     6601 gaaaattccg attggaagaa tcaaaatagc attggtagcg attcgaaaat tgatgagata
     6661 atcaacaata catgctccga atgtaaaacc aattcgtata ttgaataagt aacagacaat
     6721 ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa gaaacatgaa
     6781 actaaattca acaggctcag tgatatttgc aataaatgaa gttcctgcac cgcttcccat
     6841 gattcctttg accatctctt tattttcttt ataagctgct ttatagatag ctagacatat
     6901 agctggtata ccaaacatca ttactacaaa aaaaactgat aaaaacagtc cagcctgagg
     6961 atctccgttg ataaagcgtg tcatctctcc tctgaaaact tccttgctag gagatgtata
     7021 gcttcctaaa tcgtaataaa tatacgtatt caacacatgg tgcagtccaa aggggatcag
     7081 caaacgattc aaaaacataa atatccccac acctaatgct cccatatcaa cgagcagttt
     7141 tgcaaaatta tcaatcccaa tttggatgag cggccagata aagcccaaaa aaacagctgc
     7201 gattgtttgt agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa aataagaaaa
     7261 aactactggt aatttttgtt ctttaaagcg attatacgtc catgccgcta ctaaaccgga
     7321 aataattcca ccaaaaatcc ccatattcac ttctgggtcc ataatttcta agcctttttt
     7381 caaggtagca atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct tatttttgac
     7441 aaaaccgatt gcaactccta tagcaaataa gatatccaag tttccaaaaa cggcatttcc
     7501 agctgcacca attactggga tgttcagcat atcgtcagct gataaacgca tgatcaatcc
     7561 cgctaatggc atcgctgcaa ctggaagtag catcgctctt ccaagcttct gcacatttga
     7621 tttaacattc atttttattc ctcctctatt aaatatcctc atatgttact aactcaatca
     7681 aaggatcatt tttcactttt tgagcaaatg ctgaatgaat taaaaattcg acttggtggg
     7741 tacgaggata cacaaaactt gaaccattta acaattcctg atccaaatac gctgcgtgac
     7801 acatgacttc gatcgtttta tcttctctca atatcatgag taaacttgcc aaatattctt
     7861 ccacactcgt attgatttgt cgtttttttt catcagtttc tcctacgaca tcgaaacaag
     7921 gctcaaaata atcgacatga cgaacttcat cttttcgttc aaagttgctc cgaaccggca
     7981 aatcatattt ttttgctaat gtaacaacta cctcctgatt ttcatccaaa gtatgagtat
     8041 gatgatgact gtctaaatga gttggtatga tccccgcatg ataaattttt tgaatctgag
     8101 cattccattc ctgatacagt tgatctttgt caatgttaaa tggatgctga taataagata
     8161 aggatctgaa ctctccgctt tcgataagag tatcgacagt tttgaggagt ggtttgccac
     8221 acgtcaatgt taaatggact cctacaccta gcctaggcat ttcctttctt agtttcacag
     8281 catgctcgaa acccggcata tttgccatca atgtagttga tgtcaaaatt cctcgctgat
     8341 aagcatccat aatcccataa ttcacaccat ggctatagcc aaaatcatcc gaattaaaaa
     8401 tcacttttcc catttcattc tccttctttt ttctactaaa ataggcgcaa ccaaataatc
     8461 atagtccatt tactatgtgt catttggttg cgcctataga tagttacgcc cttattttaa
     8521 tagattcttg ctagatgaat tgccaaatag atgatttcat tttcttgaat ggcaagttga
     8581 aattgctgtt cgatgatatc tttaatcttt agtgctgtac aatacgcttt gctatgttta
     8641 aatgtttcag taaaaaaacc aatattttct actggcgttt cttcctgata aatcaagcgt
     8701 ttaaccagga atttgatatg aaccattaag cgatgataag tcagtgattc tttatcataa
     8761 acgtttgaat aggatttttc tagatgggac aaaatcgtgg tcgtcatttc tactattttt
     8821 aagctcttta aactattagg ctctcctagc tcagcattaa tgatatgcat agtgatgaat
     8881 cctacttcat ttgaccctaa ctcggtttga tatcgatgat tgattcgctc gaccacttct
     8941 actgctatat catattcatt tggataaagg aatctcattt ctggcaacat aattgctgtg
     9001 acatctctat cttctctttt tcgttgaata gcaaaataaa tatgatctgt caatgtaata
     9061 tagatatttg aatctaatgg agaggagatc ctagctgtta tcagttcaat cacttcttcc
     9121 gcaaaatcca taaattcgat tggaatctgc gagactatct cactgaatcg tttgatcttt
     9181 tctttagaat ctaatacaaa taatttgtca gcatcctcaa catcaaatgg cttactcact
     9241 ttagcagaaa agccaatccc tttccccatc gcaatttttt ctattccatc gtcatcgatc
     9301 aatacagcat tatgattcag ctttttcatc acatacaccg ttaattcacc tcctctatat
     9361 gataaaacaa aacaacttgc agactccttc atacatattt ttgtctccaa acaattttta
     9421 aagacaaaaa acgacctaag aaagaaccct ttcttaggtc gtgcctaatt gaatagtagc
     9481 accctacata atttacaaaa atagtttaac acataataaa agcgttatca atatcttttt
     9541 tactcgtttt atgataatac ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa
     9601 aaaaatatag caactctcta taagaaagtt gctatattga taattatacc ggcggccggg
     9661 gtcgaaccgg cacgtcctca acggacactg gattttgagt ccagcgcgtc tgccaattcc
     9721 gccacgccgg caaatttaaa aataataact ggggtagctg gattcgaacc aacgcatgag
     9781 ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaagg gcgaatgatg
     9841 ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc
     9901 gccatcataa aagatgttat ttaatttctc aataaaaaac taagatgaag acatcttagt
     9961 caataccggc ggccggggtc gaaccggcac gccctcaacg ggcactggat tttgagtcca
    10021 gcgcgtctgc caattccgcc acgccggcaa gttgatttat tagtactaag gcggtaaccg
    10081 gatttgaacc ggtgatgaag gttttgcaga cctctgcctt accacttggc tataccgcca
    10141 ttatactatt atcaatctat aaaataaaga ataaattata gaaactgggg tagctggatt
    10201 cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa
    10261 taaaaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt
    10321 aaccacttcg ccacattcgc catggcaggg gcagcaggaa ttgaacccac actaacggtt
    10381 ttggagaccg ctgttctacc tttaaactat gcccctataa aatggaggag agtggattcg
    10441 aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg tttagccact tcgctactcc
    10501 tccatggtgg cgcgggacag aatcgaactg ccgacacacg gagcttcaat ccgttgctct
    10561 accaactgag ctaccgcgcc atattttaaa attattaatt gaataacaag aacgacggtt
    10621 ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa cccctacacc
    10681 acggaaccag ttcattatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg
    10741 cagatgctct cccagctgag ctaaacctcc aacattcaat taaaatgacc cgtacgggat
    10801 tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca acgggccagg
    10861 taaatttgct acggagagta agggattcga acccttgaga cagtttggca ccatctacat
    10921 gatttccaat catgctcctt cggcctctcg gacaactctc cagagtgaaa gaagctcaat
    10981 actctttctc aaaatgagaa ctccggcagt aggactcgaa cctacgacat catgattaac
    11041 agtcatgcgc tactaccaac tgagctatgc cggaataaca tcagcgtggc gacgtcctac
    11101 tctcacaagg ggcaacccct cactacaatc ggcgctaaga agcttaactt ctgtgttcgg
    11161 catggttaca ggtgtatcct tctcgctatc gccaccacac tgtggtgtta tcttttattg
    11221 agtaaatttt gttcactcaa aactggattt gaagtcacat aagttttttt ccgagt
//
LOCUS       DACEJR010000091        10565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEJR010000091 DACEJR010000000
VERSION     DACEJR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.093"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            236..1042
                     /locus_tag="IW651_002532"
     CDS             236..1042
                     /locus_tag="IW651_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815217.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="IW651_002533"
     CDS             1054..2274
                     /locus_tag="IW651_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0815218.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="IW651_002534"
     CDS             2264..3850
                     /locus_tag="IW651_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0815219.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="IW651_002535"
     CDS             3889..6027
                     /locus_tag="IW651_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0815220.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="IW651_002536"
     CDS             6315..7706
                     /locus_tag="IW651_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0815221.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="IW651_002537"
     CDS             7765..8574
                     /locus_tag="IW651_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0815222.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="IW651_002538"
     CDS             8546..9535
                     /locus_tag="IW651_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0815223.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..>10565
                     /locus_tag="IW651_002539"
     CDS             9649..>10565
                     /locus_tag="IW651_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0815224.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLL"
BASE COUNT         3706 a         1580 c         2083 g         3196 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaat
//
LOCUS       DACEJR010000092        10537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEJR010000092 DACEJR010000000
VERSION     DACEJR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.094"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            90..308
                     /locus_tag="IW651_002540"
     CDS             90..308
                     /locus_tag="IW651_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815225.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(597..1109)
                     /locus_tag="IW651_002541"
     CDS             complement(597..1109)
                     /locus_tag="IW651_002541"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0815226.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IW651_002542"
     CDS             complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IW651_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0815227.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3562..3996)
                     /locus_tag="IW651_002543"
     CDS             complement(3562..3996)
                     /locus_tag="IW651_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0815228.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4006..4794)
                     /locus_tag="IW651_002544"
     CDS             complement(4006..4794)
                     /locus_tag="IW651_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0815229.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4791..5732)
                     /locus_tag="IW651_002545"
     CDS             complement(4791..5732)
                     /locus_tag="IW651_002545"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0815230.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5909..6496)
                     /locus_tag="IW651_002546"
     CDS             complement(5909..6496)
                     /locus_tag="IW651_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0815231.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IW651_002547"
     CDS             complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IW651_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0815232.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVQDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8318..9328
                     /gene="gap"
                     /locus_tag="IW651_002548"
     CDS             8318..9328
                     /gene="gap"
                     /locus_tag="IW651_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0815233.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9413..10348)
                     /locus_tag="IW651_002549"
     CDS             complement(9413..10348)
                     /locus_tag="IW651_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0815234.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2934 a         2368 c         1827 g         3408 t
ORIGIN      
        1 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
       61 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
      121 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
      181 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
      241 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
      301 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
      361 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
      421 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
      481 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
      541 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
      601 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
      661 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
      721 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
      781 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
      841 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
      901 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
      961 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     1021 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     1081 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     1141 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     1201 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     1261 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     1321 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     1381 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     1441 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     1501 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     1561 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     1621 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     1681 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     1741 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     1801 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     1861 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     1921 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     1981 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     2041 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     2101 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     2161 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     2221 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     2281 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     2341 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     2401 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     2461 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     2521 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     2581 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     2641 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     2701 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     2761 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     2821 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     2881 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     2941 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     3001 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     3061 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     3121 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     3181 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     3241 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     3301 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     3361 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     3421 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     3481 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     3541 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     3601 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     3661 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     3721 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     3781 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     3841 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     3901 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     3961 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     4021 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     4081 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     4141 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     4201 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     4261 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     4321 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     4381 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     4441 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     4501 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     4561 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     4621 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     4681 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     4741 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     4801 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     4861 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     4921 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     4981 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     5041 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     5101 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     5161 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     5221 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     5281 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     5341 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     5401 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     5461 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     5521 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     5581 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     5641 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     5701 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     5761 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     5821 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     5881 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     5941 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     6001 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     6061 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     6121 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     6181 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     6241 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     6301 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     6361 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     6421 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     6481 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
     6541 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
     6601 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
     6661 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
     6721 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
     6781 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
     6841 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
     6901 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
     6961 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
     7021 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
     7081 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
     7141 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
     7201 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
     7261 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
     7321 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
     7381 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
     7441 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
     7501 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
     7561 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
     7621 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
     7681 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatcttgg
     7741 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
     7801 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
     7861 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
     7921 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
     7981 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
     8041 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
     8101 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
     8161 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
     8221 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
     8281 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
     8341 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
     8401 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
     8461 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
     8521 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
     8581 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
     8641 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
     8701 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
     8761 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
     8821 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
     8881 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
     8941 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
     9001 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
     9061 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
     9121 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
     9181 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
     9241 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
     9301 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
     9361 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
     9421 catattggct taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
     9481 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
     9541 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
     9601 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
     9661 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
     9721 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
     9781 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
     9841 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
     9901 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
     9961 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
    10021 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
    10081 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
    10141 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
    10201 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
    10261 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
    10321 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
    10381 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataacccgc
    10441 gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt
    10501 ccgacgaaag aaaacaaagg agtgtaacca taaatga
//
LOCUS       DACEJR010000093         9848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEJR010000093 DACEJR010000000
VERSION     DACEJR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.095"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            471..2108
                     /locus_tag="IW651_002550"
     CDS             471..2108
                     /locus_tag="IW651_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0815235.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW651_002551"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW651_002551"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0815236.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IW651_002552"
     CDS             complement(3184..3819)
                     /locus_tag="IW651_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0815237.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW651_002553"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW651_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0815238.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="IW651_002554"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="IW651_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..8465
                     /locus_tag="IW651_002555"
     CDS             6417..8465
                     /locus_tag="IW651_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0815239.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIGSLSNDVGKEKS
                     "
     gene            complement(8512..9807)
                     /locus_tag="IW651_002556"
     CDS             complement(8512..9807)
                     /locus_tag="IW651_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0815240.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         3215 a         1814 c         1575 g         3244 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaataggct ctttgtcaaa tgatgttggt aaagagaaat
     8461 cttagaataa tttggacaga acagaaagaa gaaaacctct ttctgttctg tttatttggt
     8521 taaaaatgtt ttgccatgac gaatcataat tcgtagtttg aaattgtaaa agttgcgaaa
     8581 gccgtacgca ttgcgtttta atactttgat gtggttgttt aagcattcca atggtccgtt
     8641 tgaatagctg tagtgaagcg cattttggat ctgtggcaga aactttttaa acgttcgtaa
     8701 agtggttgta taggtttctg gcagaccgct aatgtctcta gttaaacaat cctcaaatag
     8761 tgactgatct cttttcttta ccgcataaag aatgtcttgg tataactgat agccttggcg
     8821 taagttggga caagcagcta acagtcgatc ggcaatctca ctttcagtca aataatcttt
     8881 aaaactggca cgccaaaccc gtttttcata gtttaacttt gcttcttctt tttgtaggag
     8941 tttccaatag cgcttcaatt ttttccctaa ccctcgttga gagtgggaat ttcctttaag
     9001 tcgtaccgtg gtttctttca ctcgatgatt caagaaagtt ctgccaatat gttggacgat
     9061 gtggaaacga tcgatgatca actgagcatt cgggaaaagc tttttcacca acgaaacata
     9121 aggcgcatac atatcaataa caatcaattg aacggcttca cgagtcgcta atgaaaaccg
     9181 agaaaaatag cgttctaaat aaggcaagcg tcgattttcg acaatatcca ataatgcatg
     9241 gctttgtccg tccatcatga taaaactcat tgcactggca acacttcgaa ccgatttgaa
     9301 ttcatcaaag cataagacct ttggcaaagt ctgtttgaaa ggattcaatg ggcggtagaa
     9361 tcgcttcatg acacgataga ccgaagaagt ggaaatattt ttctgacgag cgatttccgt
     9421 catggaagta ttctctttta agcgagcaat gacagaccaa cgaacttttt gactaatgaa
     9481 acagtgtttt tcagcaacag aggtatccgc tacgaatgtt ctttggcaag acttacattt
     9541 aaatcgttgt ttcttcagct ccagataagt gcgatactca gacacatcat tcagtaacaa
     9601 ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa gacaatggga
     9661 acactcttct ggcttgtaca gaagagttcc tttgtaaatc aagcaaattt gccctttgta
     9721 tttttcttta gttaaacaat tttcctcgaa atagatattt aggtctagaa tatttagggt
     9781 atctttgata agctgagtat gagacatctt gaatcctcct atgaattggt agccaaatta
     9841 attctaaa
//
LOCUS       DACEJR010000094         9622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEJR010000094 DACEJR010000000
VERSION     DACEJR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.097"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..2427
                     /locus_tag="IW651_002557"
     CDS             100..2427
                     /locus_tag="IW651_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0815241.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IW651_002558"
     CDS             2553..2882
                     /locus_tag="IW651_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0815242.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IW651_002559"
     CDS             2922..3926
                     /locus_tag="IW651_002559"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0815243.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IW651_002560"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IW651_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IW651_002561"
     CDS             5558..6730
                     /locus_tag="IW651_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0815244.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IW651_002562"
     CDS             complement(6892..7347)
                     /locus_tag="IW651_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815245.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IW651_002563"
     CDS             7936..8358
                     /locus_tag="IW651_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0815246.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..>9622)
                     /locus_tag="IW651_002564"
     CDS             complement(8524..>9622)
                     /locus_tag="IW651_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0815247.1"
                     /translation="MITSFHISGTQAGFISTITNLGMLIGGIFFGIIADKYGRVKVFS
                     QTVLLFSIASLLMYFASNIYLVYLFRFIAGVGAGGEYGACMSLISESFSKKQIGRASS
                     VAGIGAQVGAALAAILAAVIIPTFGWKMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQS
                     AEKKTKLSRLFESKHLAWQTCGLSIMVTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSL
                     WMVSTILGMSLGMMTFGIIMDKLGPKLAFSLFLICSSLSVYLLVLAHSQWSLVAAAVI
                     VGYFINGMYGGYGAVISSLYPTEIRATANNFIMNLGRAVGGFSSIVIGFLMDHYSLTA
                     VILFLSGIYLVSLLVLTTLTGVTALRESLQH"
BASE COUNT         3304 a         1831 c         1857 g         2630 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca tt
//
LOCUS       DACEJR010000095         9621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEJR010000095 DACEJR010000000
VERSION     DACEJR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.098"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW651_002565"
     CDS             168..362
                     /locus_tag="IW651_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815248.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW651_002566"
     CDS             631..930
                     /locus_tag="IW651_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815249.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW651_002567"
     CDS             complement(1027..1461)
                     /locus_tag="IW651_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0815250.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW651_002568"
     CDS             complement(1693..2403)
                     /locus_tag="IW651_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0815251.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW651_002569"
     CDS             complement(2393..3253)
                     /locus_tag="IW651_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0815252.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW651_002570"
     CDS             complement(3258..3896)
                     /locus_tag="IW651_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0815253.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW651_002571"
     CDS             complement(3911..4210)
                     /locus_tag="IW651_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0815254.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW651_002572"
     CDS             complement(4253..5737)
                     /locus_tag="IW651_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0815255.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW651_002573"
     CDS             complement(5757..6218)
                     /locus_tag="IW651_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0815256.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW651_002574"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW651_002574"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0815257.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW651_002575"
     CDS             7521..8291
                     /locus_tag="IW651_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0815258.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW651_002576"
     CDS             complement(8564..9391)
                     /locus_tag="IW651_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815259.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENNLK"
BASE COUNT         3007 a         1954 c         1504 g         3156 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggttgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat a
//
LOCUS       DACEJR010000096         9324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEJR010000096 DACEJR010000000
VERSION     DACEJR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.099"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..227
                     /locus_tag="IW651_002577"
                     /pseudo
     CDS             1..227
                     /locus_tag="IW651_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209247.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            229..1527
                     /locus_tag="IW651_002578"
     CDS             229..1527
                     /locus_tag="IW651_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0815260.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2174..2854
                     /locus_tag="IW651_002579"
                     /pseudo
     CDS             2174..2854
                     /locus_tag="IW651_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            2882..3445
                     /locus_tag="IW651_002580"
     CDS             2882..3445
                     /locus_tag="IW651_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815261.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(3490..3657)
                     /locus_tag="IW651_002581"
     CDS             complement(3490..3657)
                     /locus_tag="IW651_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0815262.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(3691..3990)
                     /locus_tag="IW651_002582"
     CDS             complement(3691..3990)
                     /locus_tag="IW651_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815263.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(4031..4648)
                     /locus_tag="IW651_002583"
     CDS             complement(4031..4648)
                     /locus_tag="IW651_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815264.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            complement(4973..5368)
                     /locus_tag="IW651_002584"
     CDS             complement(4973..5368)
                     /locus_tag="IW651_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815265.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            complement(5448..7799)
                     /locus_tag="IW651_002585"
     CDS             complement(5448..7799)
                     /locus_tag="IW651_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0815266.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            complement(7924..8613)
                     /locus_tag="IW651_002586"
     CDS             complement(7924..8613)
                     /locus_tag="IW651_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815267.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            complement(8627..9079)
                     /locus_tag="IW651_002587"
     CDS             complement(8627..9079)
                     /locus_tag="IW651_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815268.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            9210..>9324
                     /locus_tag="IW651_002588"
     CDS             9210..>9324
                     /locus_tag="IW651_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0815269.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
BASE COUNT         3077 a         1779 c         1444 g         3024 t
ORIGIN      
        1 acagcgaaaa tccgaacatt ttcacctctc tcactttata acttttacaa aatagtactt
       61 ctcataaaaa gcgctctagt taaagaaaat aagagaaaaa tcaaaaaaaa caaaagtttc
      121 ttttacactt gtttctatat tcgttacaat ggttatggga agtttttaaa aaggttaaga
      181 aaagcttacg ggggtttttg aaagcaaaaa gtagtagggg acgataaaat gaaacataca
      241 cacaaaatca tattggctgt cacttctgta tgcactgtta ttattctagg tgtaagtatc
      301 ttttttgtta cacaagcagc aacaaacaat aaattagaaa acactagtca aacaactcaa
      361 cactcaagca gcagctcaaa acctgttgaa gataaacaaa cgacgaaaca gcttgatcaa
      421 gcaaaacagc ttgctgcaag ttatcattat gatgaagcca tcgccttact tgaaaaagac
      481 gacgccaaag aagcgcaaca attgttagca actttgaaaa aagaaaaaga atccttggta
      541 aaatgggaag acccgaccaa aatctcacac gtcttctttc atagtctgat cgttgatcca
      601 gctaaggctt ttcataccca gcaagcacaa gggtataaag attatatggt cactatttcc
      661 gaattcaata aaaccattga tcagttatat aaaaataact atgttcttgt taatctaaat
      721 ggattagtca aaaaaggaac agatggaaaa ctgaccttta caggtgtttc tcttccagaa
      781 ggaaagaaac cgttaattct ttcgcaagac gatgtcagct attatgaata catggacaat
      841 tcaggatttc caagtaagtt gattgtcgat aaacagaacc aaatcaagaa tatttatatc
      901 gacaataaga aggaaactgt tggtgactat gatatggttc cattgatcga ttcattcatt
      961 aaaaaacatc ccgatttttc ttatcaaggc gccaaaggaa cattagcttt aacaggctat
     1021 aatggtgtat taggttaccg tacatcaaaa tcagaatatg gtgataacga aaaaacacac
     1081 aaagaaatcg aagcagccaa aaaagttgct gatcaattaa aggaagatgg ttggagtttt
     1141 gcttctcata cttggggaca cttgaatatg acacaagctt ctttagccga tatccaacaa
     1201 gataatgaac gttggcaaaa tgaagttgca cctattttag gcaaaacaaa tattttgatc
     1261 tacccatttg gcgctgacat cagcgattgg caaccttatt ctgaagcaaa tcaaaaattt
     1321 gcttacttga aacaacaagg atttgatatc ttctgcaatg tcgatgcctc aactcctgca
     1381 tggggacaac taggaactga ctactaccgt aacgccagaa tcaatattga cggtatccgt
     1441 ttcgaagctg acctaaaagg agaaaatcca atacttgatc actttatcaa cgtcaaagaa
     1501 atgtatgacc aaaaagatcg aggatagagg ttgtgaaaga agcgtccagc tccgagcaaa
     1561 taagaactag atttctaaaa tagtttctca tattttggaa atctagcgct tattgctgag
     1621 gagttgcttc ttgaacaccg tgaatagagg ttgtaaaaag agcgttttga cttattgttg
     1681 aaggtcagct ctttgaacac cgcgtgttag gttgtgaaaa ggctggtctg catcaagcaa
     1741 taagaatgaa cagagaaaaa cagcttcttg aataccgcag ataggttgta aaaaaaccgt
     1801 tttgacctga cgataaccag taaaacagag gtgagaccat tgctaaaagt agcagtggtc
     1861 tcatctcttt ttttgcacaa gtcatatttg attgtttcgt tattttaatc aggataaagt
     1921 tctttttttc atgctaaaga gtgattcttt cttctcgatt ttcactttct tcgtttaact
     1981 tttggtaaat acttctgtca gaaaaagctc taggtagaca cacgaaagca gataaaaagc
     2041 ctaacttttg caacagttta tgctgtcgtt caaagttagg cttttacagt ttataaatag
     2101 ggttctgttg caaagttttc caaaaaatct attttagtgt aaaattgaga aaaaagacag
     2161 agaggacaga gtaatgaatc attttaaagg caaacaattc aaaaaagacg tcattattgt
     2221 cgctgttggt tactacctgc gttacaatct aagctatcgt taagttcagg aattgttata
     2281 tgatcgtgga ataaatgttt gtcatactac gatttatcgt tgggtacaag agtacagcaa
     2341 agtcctctat tatctttgga agaagaaaaa tagacaatcc ttctattcat ggaaaatgga
     2401 cgaaacctat atcaaaatta agggacgttg gcattatctt tatcgtgcaa ttgatgcgga
     2461 cggcttaacc ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt
     2521 cttaaaacga ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc
     2581 ttctcttggc tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca
     2641 ccgaactgtg aagtatctta acaatttaat agaacaagac catcgaccta ttaaacgacg
     2701 aaataaattt tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat
     2761 tcgaggaata tataaaaaga accgaagaaa tggaacgctc ttcggctttt cggtgtctac
     2821 tgaaatcaag gtattaatgg gaataacagc ctaagatatt tggagttcag agagggcgcg
     2881 tttgattttc aaacttcgca acagaaccga aggacttaac attgataaat tattccaaga
     2941 aaaagccagt ggtaaagata ctgagcgtcc tgagtttcaa aaattactaa attatatccg
     3001 tgaaggtgat tgcgtcatcg ttacatcact agatcgttta ggaagagatt atgaagatat
     3061 aaaaaataca gttgctttta tgaaacagaa aaaagtagcg ttaaaaatat tagatgcaca
     3121 gtttttagat tttaatactg gaaatgaact cctggatacc gctatgttcg atatgttttt
     3181 atcactgcta agttatattg cacaaaacga gcgtgagaaa attcgtgaac gtcagcggca
     3241 aggagtatta ttggctcaaa aagccggccg atataaggga cgaccaatcg agtattctat
     3301 taactcaaca gatccgcaaa aacgcttagt ttttaaaacc gtggtggaca tgttaaagca
     3361 aggcattcca gttgcagaga ttgcaaaaga aaacgggatc tctcgaccaa ctatttataa
     3421 aataaagaaa gcgaatgatt tgtagaaata tactacattc attcgctttt ctaccgcact
     3481 atttcgtttc tatgttccat gtaccccgta tcggcgatgg catttaggac agtaaaagta
     3541 atttttttga taaacacttt ttgctaattc cccacagtca cactccacgt gaaaagcaat
     3601 atttcgatta aaactagtta tttcaatgcc ccaacgatca taagattttc gcttcataaa
     3661 aattttcctc cttgtaaatt ttactaacga ttataaatac cgttgccatt ctttcaactg
     3721 ttgactaatt aacgcataaa attcgacttc tgataaaatg gtaatctcat gacctaacgc
     3781 aatcaagtcc cgcgcggttt gttccttttt tgtatagaga gaatcatcaa acaacgactt
     3841 agaatactga ccacaaacca ggaaatttgt ttttgaagta acacttgatt gacaatggcc
     3901 gtttaaagag tgaaccaaac cgatggcttg ggttcgtgtc atttgctgca gcgttccagt
     3961 aaaaacgacc ctttgattcg ttaagtccat tttcaaactc tcccttcccg tctaatagct
     4021 gttatatcta tcaagaaaag tggtgcaagg tatgttcata ttctttcacc cgtcgataaa
     4081 aggtatttcg tttcatgccc aattgatcca tagcatagac tgcagttatc tttcctgctt
     4141 tccagtcaga atagatcgca ggaaattcag gaggtaaagc aattttcttt ctgccgaatt
     4201 ttacaccctt ttgtttcgca gcttctacgc cttctctttg ccggctatta attcgtttcc
     4261 gctcgtcttc ggccatatag ctgagtaatt gcagaattaa gtcagagata agcgtctcta
     4321 acccatctag ttgcttgtaa ctagcagtat ctaacagagg catgtctaac acagaaatat
     4381 caatctgctt ttccttagtt aattctcgcc attcttctaa aatcatttgt ttatttctgc
     4441 ctagtctatc taatgattgt agatagagta aatctccttt tcgaaacatc cgtttcatta
     4501 attgatattc agggcgttta aaatctttgc cgctggcttt ttctacaaat atatattctt
     4561 cagggattcc taattccttc gcggaaatca gttgtcgttc gatcgattga tcataactac
     4621 tgacccggat ataaaaaaat tttctcatcg tttttatcta cccttttttc actaaaattg
     4681 tttcaaaaca atcagattaa atcagaaacg tttcaaattg tattatgtgg ttattatgcc
     4741 atacataaac aaagaaaatc aaggcatgtc aaaaagtata ctttttgaaa cagtaagttt
     4801 acactcccgc atgattatat ttattccgct aaaatacata aaaatggcag gccttaacgg
     4861 tctgccatta aataaactag ttatccagtt tttcttttat gtcttccatg gtatatttta
     4921 tttttcacat cagaatttga cttctttaac ttttacaatt tttttaacac tattattcta
     4981 ttacaacact atatatttct aatttagggt taaaaataaa tctttttgat acttttgcct
     5041 ttacaatata attgtattct ttcttttttt ctttgaagtc gcttgattca actgtaactt
     5101 caaaccacat ttcatttgga gactctttta atttttttac ttgaaatgaa ttatctcttg
     5161 gatcataagg tggtactctt tcaaactcag gaagttttct ctgtatttgt tcttcaggag
     5221 tattcgcaaa gtaatctact atcttgacag ttattaaaag tactactatt cccaaaacta
     5281 taacaaacat aaagataatt ccacatccag aattattttt attattttga gtcagattat
     5341 tccattctat ttcttccata atcatcatgt ccttttacaa ttcatatact tattttatcg
     5401 tatatattta aaaaggaaaa ggacactgtt taaatagtaa acgattctta attaagttta
     5461 cttctatttt cttgaaattg aataattttt tcagaaaatc catttgcaac tttcccctca
     5521 tctccaataa aatcaagata agtaaaatct gtcccaagtg aattattaac tattttatca
     5581 atgaaaaatg aaattatccc tacacctaag atatcaactg tttcgtcatt ttctaataaa
     5641 ctatttgtta ctgaagcgat tttatctgaa gaaccaaatc tgttcttaac aaattgatta
     5701 aatctatcaa tagcaccctt atcacaatag tacgttttta aagccgataa agctgttgaa
     5761 tcagaatttc tttttgctaa ggtataaaca tttaccgcgt caacatcttg gtataaatct
     5821 tctagactaa atgttccacc tgcagccgta cctactaatg caaatacacc ttgctccatt
     5881 tgtattccta atttcttaac ttcaccccag caagtcatta aatctcctaa ccaacctgca
     5941 aagtcagaaa cactatcatt gattgtgtcg gctagaaaca tctgtgattg taacgtcaca
     6001 attaagtgag gcaaacctat tattgaacca gtattaggat ctataaaata attatacagg
     6061 ccctcgctat tagttaattt attcctatat tcatcataaa tttttctatc atgctctgtt
     6121 acaggagaag aaatgatgtc ccatgacaaa ccagaataat ttacatatct ataaaatgtc
     6181 aaatgagggt tccaaggatt atcaagacca gcttgtgata gatattgata cgcacgtacc
     6241 atctgattta taaccttctg attgtatttg gcaatgtaat tatcattatt agtatcattt
     6301 gataactcat taaatccgct atctctaccc gatgtagcga ttaaatcgat accaacagct
     6361 ccgttgccac ttcctatggt aaattcaaca aattgatcaa aagaccattg tctaggcata
     6421 ggaaaaccta aattcccact ataaccagtc gacatatctg aaacaaatgc aaacgaagca
     6481 tacccgttat tgattatttg agaacaaaca tttcttgttc cgtaaacacc cgtttgatac
     6541 ccgctatatg acattacagc agataccccc ttaaaatatt ctccagcata tgcaagaatt
     6601 tcttccccta acatgtctag atcaacagca aaataaataa ctgttgttaa tggtatattt
     6661 agattttttg ctgcatctat agccttctta ccatctgata agccacgatc ataagtaaaa
     6721 taagctaatg ctgcacctcc atcttgatag ataggaaaca cagataaatt tttagaaaaa
     6781 atattattca attcctctgt agttagatac ttatttcgtt catcttttcc aattccaaca
     6841 gtaccagtaa gatatctacc aactattttt atatcattat caaccaatgt ttggatttga
     6901 gatctagtta attgcgtaga agtatctgcg ccatctgcag atcttctaat atctccagcg
     6961 ctagacaata gtccttttat tacaacaaca tctgctattt cagtatattc aggcaatacc
     7021 ataaattttc ggaatgccag tacttctaaa cccatatatt tagtgaaaat accatcgaat
     7081 gtaccgtctt caaaataacc attaacatat aatccatatt gaattattct tacatagttt
     7141 cccgagttac cttcagacac aggataagtt ttactagttg tcccattacc ataagaccct
     7201 gttgcttcac tgggagaata tcctagttca gcttgtaatg caaaaattaa tgcttgattt
     7261 gaatttcttt gataaatacc atcacaaggc attattcccg tgtattctaa ataatttaca
     7321 tttaaccatt gctgcatttt tctaatttta ctatctccgc cgggaacaag aacaaaagca
     7381 ctcatatcaa ataacgcttt ggcccatttc gcatccaaaa ctccagttac agcgcttcca
     7441 ttaaaccctg catcagtctg caactctttt acagcagtat ctaaatatgg agtatatacc
     7501 ccatcgaagc cacctggatt aatcccctta caccaaaagg caccttgaat aagaaataca
     7561 atatttttag gtagattttt gccccattta ggagtaacct gattatcgta cattttttca
     7621 gtggtaggtc caaaattagc tactagatta gtaataccaa gttctacttg tagtccttca
     7681 ataagtccat agattgtagg ccaccctgtt cgcccatttt ctggaacctt atcaaaccct
     7741 gtaacattac tgtatttctt gtttagccat ttctgagtac ttaaaaccat ctcatccata
     7801 aaaatcccct gctttcttgc cgaacatacg ttcgtttatg tgttgattat attttatatt
     7861 ttagtgtttg tcaacaacta aaaataataa aacagcacaa agaaatctct ttgtgctgtt
     7921 ttattaaata ttttgaacaa cataatacgt acctgcaaca ggattataag tgtaataaat
     7981 acctatattc attgtccaac caggcccgtt tggtgcaaag tatttgaatc cttcacgagc
     8041 atacaatgcc attccttgaa tagaaccaga tttagttgcg ttggcttgat ttacatttcc
     8101 tactcttcca tcgtcaatgt aggatttcca acgtaaaaag ttgcctgtac catcagctgg
     8161 ttttacaccc atacctccaa aacgccatcc cgcaccacta aatggctgtg atttatagct
     8221 tcctcctcct gctagtacaa gaaggtgtcc gctagtacca acatcgggtg gaaatgctcc
     8281 tcgaggcata attggagtag tttcaatatc atttttgaat ttttcagcaa caattttttt
     8341 tgcttctgtc actgaaactg aagcaggatc agtttctgaa ttatatgaac cttgaattat
     8401 atctggatta tccatagata caattttagc ttcccctttt aaaaaaccac cttctggcaa
     8461 tgcaagtact ttatcttctt cttcaaaatc agcaaagtat cgtgaggcgt actgctctgg
     8521 atctgaaata acttctttct cagcagcata tactgcattt ccaaatgtta acatcataag
     8581 tagcccaaca gaaacaccaa aaatcttttt cataatgaat cttcctttac tctaattcca
     8641 ctgaattagt ttcttttatc tgctttaatt catttctttt actatttaaa agctctttta
     8701 gatccttaat cgaaatagcg ttggaatcag tattaacatc aataaactgt ctgtgatttt
     8761 gtttataact aactaattcg gatttgccat ctcccattaa ttgcccacca gcagacaaga
     8821 caacaatttt atctgagtca ctaattcctg gaatactttc ttctaactgt tccccggata
     8881 aatcaccaaa ttctttatac aattcttcat ctgacataga atagatattt ttggtcaaac
     8941 tcattttttt attattatgt tcagctactc tttgatcttt tatttgagcg tttgaattaa
     9001 cagaatctaa attcaaaaga aaatagcccc caattaatat tgtaaatact gaaaaaatac
     9061 ttagtatcgt ttttttcacg attctcacct cgctttctta ttaaatttat aatatatatc
     9121 tacgataaga taattggttc tgttgcaaag ttttaaatct actatcaaat aagatagaat
     9181 aatagaaaaa gatagcagga ggaataacga tgaatcattt taaaggaaag caatttcagc
     9241 aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc tatcgtgaag
     9301 ttcaagaaat cttatatgat cgtg
//
LOCUS       DACEJR010000097         8824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEJR010000097 DACEJR010000000
VERSION     DACEJR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.100"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..524)
                     /gene="nrdI"
                     /locus_tag="IW651_002589"
     CDS             complement(81..524)
                     /gene="nrdI"
                     /locus_tag="IW651_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0815270.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            671..1336
                     /locus_tag="IW651_002590"
     CDS             671..1336
                     /locus_tag="IW651_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0815271.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            1326..1781
                     /locus_tag="IW651_002591"
     CDS             1326..1781
                     /locus_tag="IW651_002591"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAQ0815272.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            1931..3310
                     /locus_tag="IW651_002592"
     CDS             1931..3310
                     /locus_tag="IW651_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAQ0815273.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            3447..4289
                     /locus_tag="IW651_002593"
     CDS             3447..4289
                     /locus_tag="IW651_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0815274.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            4363..5811
                     /locus_tag="IW651_002594"
                     /pseudo
     CDS             4363..5811
                     /locus_tag="IW651_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            5926..7698
                     /locus_tag="IW651_002595"
     CDS             5926..7698
                     /locus_tag="IW651_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0815275.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            7673..>8824
                     /locus_tag="IW651_002596"
     CDS             7673..>8824
                     /locus_tag="IW651_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HAQ0815276.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGY"
BASE COUNT         3063 a         1455 c         1685 g         2621 t
ORIGIN      
        1 attgctttat agtgtaaata attatttatc atagtacctc ctaaaatatt gtttgaaagg
       61 ggatgatatg gtgtcatttt ttattgcatt gtttctgcaa ttctctctat atcttctgtc
      121 gtacctttta gttcaaattc atccaacacg gtaaaaccaa aatcatcggc gtattgatag
      181 gctgttaggc aaaattgttt attgaagttt ctgttgccac taccaataat tcctaaacaa
      241 tagtctcggt ttttattgta agtaatcact tggcgcaatg gtgttgtcaa aatttcgaca
      301 tctcctgttt caataccatt tccaccttca agataagtag gcagaaatac aaaataggat
      361 tcattgatgt caaaagtgag tggttttcct aatttgacta aatcttttac attcacataa
      421 tctaccttct tttgatattg tttttgacaa tacgtatcta aatctgcgag aaaactcgtg
      481 gtatttccac ttaaactaat ataatagact ttaattattt gcatgtatca cacctctttt
      541 taataaataa ccacatatat agtgcttgtt ttaattagaa acgctatata tagtgtttat
      601 tgtacatgac atatcgttat agaaaattga cgatggtcaa ttattttgat tttagaggag
      661 agaaaattac atgaaaaatt caggttgttt atgcaccgtt ccactcttcg cctctctttc
      721 attagatgaa aaaaatgaga taaagtgttt gcttcaccat cagacgttta aaaaaggaga
      781 aattattttt tcaccaagta gtgaagaaca actgttgatt atatctttag gggctatgaa
      841 aatatatcga attagtagaa ctgggaagga gcaattaatt cgtatcgtaa aagaagggga
      901 ttacgacggt gagaactatc tctttggtct acccaatgac tgcctatatg gtgaagctgt
      961 tgaagaaacc aaaacttgca ctctctataa atccgacttt aataaagtga tggcacgcta
     1021 tccacagctc agttatcgat tgttacaatt aaatgcttgc aaaacagtag ctgtggaaaa
     1081 acagttgcag cttctagcgt ttgatcgtat agaggaccgg ttagccttat atcttaacca
     1141 actatctata gcagcaaaaa tgggggagcc tattacttta cctatctcta tgagagagtt
     1201 ggcaaactat ctagctacca gtcctgaaac tatttctcgt aaattaagag attttcaaga
     1261 aagacaattg attactagaa aaggaaagca gattacacta ttagaagaat tttggtttat
     1321 atttaattat atataaagaa gaagatatta aaattgggat tgtcattgca cgattcaatg
     1381 attttttaac taaaagctta ctaagtggtt gtgaagatgg ccttacgcgt tcaggcgttt
     1441 tgacagaaaa tatcgatgtt gcttgggtgc cgggggccta tgaaatccca cttacagccg
     1501 caacgatggc tgaaacaggt aaatatgatg ccattattac cctaggtgcg gtaatcagag
     1561 gagctacaag tcactttgat gtggtggtta accaagcgtc aagtggggta tctcaagtag
     1621 gcttaaccca taaattaccg gtcatctttg gtatcttaac cgttgaaaac atcgaacaag
     1681 ccattgaacg ctctggtact aaagctggta accttgggtt tacttatgca acgaatacta
     1741 ttgaaatgat taatttattc aaagaaatta aacaacaata agtcatcatt ctatatgaaa
     1801 atatctgtaa cagcataggg ctagctttaa ttttcaatca tggtaagatg gaagatagga
     1861 aaataaagcg cattcaatta attatcaagc ctttctgatg atttgcgcaa aaagcttaag
     1921 gagacttcat atgacgattc aatttaaaca aacattagcg ccaaccaagg ataattctgg
     1981 aaaaccaatc attttaagtg acgctacctt cgctcaacgt aatgagaaag tccttcagtt
     2041 aatgcgtgaa tatgacttct catcattgat gatttatgca gataaagaac acggtagtaa
     2101 ctttgaatat ttaactggtt ttattccacg gtttgaagaa gctatccaag taatcaacca
     2161 agacggaacg tcgacactga ttttaggtaa tgagaattac aataaaacca gtatggcacg
     2221 tacacaaagt gaaggtattc tttgcccatt cttctcttta ccaaaccaac ctatggattt
     2281 cactaaaact tttgctgact atcttaaaga cgttaaagta gatgcttcaa aacgaattgg
     2341 tttagtagac tggaagcttt tatctaatga tatggacaac ttccacaacc atcattcaat
     2401 accagcattt attgtagatg gtatacatga agttttcggt tctgacaagg tagtgaatgc
     2461 aacccaacta tacatgcatc caaaagaagg tgctcgtgtg acaaacaacg cagaggaaat
     2521 cgctcgttat gaatatggtg cttcccttgc ttctgatgct gttcttgaag caatgaacca
     2581 gctaaccgtt ggccaaagtg agttagcagc aggtaatcac ttaaatcgcg atggtcaata
     2641 tccatctgtt gtgaccattg cagcctttgg tgatcgcttt gagggtgcta atatttatcc
     2701 aactgataat acgctaaatg atggcgataa agttgcatta actgtagcct ataaaggagg
     2761 cttgtctagc cgctctggct atgcagtaaa agaggaaagt cagctcgctg aacatgatcc
     2821 aggatactta gaagaagtgg tttacccata tttccaagcc tataaatggt ggctagataa
     2881 tttaaccatt gggagaaagg gtggcgattt ctaccaagca ttcagtgact tctatccgca
     2941 agctacttat ggctggaatt tgtgcccagg gcatttagta gctgacgaag aatggttaag
     3001 ctcaccattt tatcaagact ctgatgcaac aattcaaagt ggctacatct tccaactaga
     3061 ctttattcct tcacaagcag gccacaatgg tgtttcagct gaatcaacga ttgcggttgc
     3121 ggacgcggca ctacgtgaac agattaaggc tgaacaacct gaattatggg cacgtatctg
     3181 taaccgaaag gattatctaa aagaagcatt aggtttagaa ttatcagaac atttactacc
     3241 cttagcgagt acctttgcct actatagacc tttcttatta gacaaaaaaa ctgctatcta
     3301 ctttggataa aaaaatgccc ctcaagattg tttgccaaac ttgagggttt tttgtctatt
     3361 tttaagtgaa taatgacgga ctatccaaac ggaatcgcca taaagatgtt aatatatgta
     3421 taatattata aagaaattgg aggacaatgc ccttggacga gcagattgaa ttattattta
     3481 ccatagatga aggctatttg aatcccttaa aagtggcttt aacatcgatt cgtcagaata
     3541 atccaggcca agctttccgc atttggttga ttcacgaatc cattgcaatg catacgattc
     3601 gcgaattgca gaagctaacg gattatctac agtttggttt tgaagcgatt aaaattgatg
     3661 gtagtcgatg gaatagcgct aaaactgaag atagataccc aaaagagatg tatttccgcc
     3721 tgcttgcggg tgaaatatta cctaaagaaa tgaaacgcgt gatctattta gacccagata
     3781 ttttagtggt taacccttta ttagatttgt ggcaaacaga tttagaaggt tatatgctag
     3841 cagctgctac acatgttgga ttgacggacg tttctacacg agtaaaccaa atgcgacttg
     3901 acgttgacca tgcttattac aattctggtg tgatggtgat tgatttagat aaagctagag
     3961 aaattattaa atggtcagat attgcacaaa tgattgaaaa atataacttg ctattaatcc
     4021 tacccgacca ggatatcctc aaccgattat atggtaaata cactaaggaa attccagaag
     4081 aaatttggaa ttatgatacg cgtaaatata tgcggtattt caccaaaagt ctcgctcaac
     4141 atgatattca ctgggtgatg gcgcatactg ctattctcca tttctgtggt ggcccaaaac
     4201 catgggacga taaacatgat aatcgtttta ccagtcttta tcttacctat caaaatcaat
     4261 taaagcgtat tgaagatttg atgaagtaaa gtgaaacaag tgtttttcat tacctacggt
     4321 ggtttaatgc ttatttatta tcgttaaatc atgcttatac actttacaaa atttgatttt
     4381 cgaaatggcc gtccaacgaa gtctgcgatc ttaaccacga gttccattga catggccaaa
     4441 aagtattatc gagcgatcaa agaaatgacg aaagaacctg actggttaac aaaagagttt
     4501 cctaatcagc ccattcgtga aggaagaacg atggaggatc ctgattttcc acgaatcgct
     4561 attacctatt cattagaaga aaataaccaa gattcaagtg cccaacaaga agaaatgaaa
     4621 aaaattattg aggaatataa tcagtatttt gatactgcct ggtcattagc agacattgag
     4681 cgttataacg gagacatcaa taattgtttg gcacgaaaaa gagcggaatt caaacaattt
     4741 gggaaacaaa tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcacct
     4801 acgattcaaa ccctatttgt ggatcgtaat ctagagtatg caggattgat tcaagctttt
     4861 tcacgtacga accgtacgta tcctgaaaag acaaaaggat tgattgtgac gtttcgtaag
     4921 ccagcgacaa tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agcaaaagaa
     4981 gaatcgggtt tgatttatcc aagttatcag gaatccaaaa aaagattcaa gcaagcgtat
     5041 ggtaagttaa aggaggtttc atccatgaaa tccattgatg aacatacgcc acttgaaatc
     5101 cgtatcgagt acgttaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact
     5161 tatgacgatt acaatgatga catggtgcag tcttcaactt tgaacaatca aattcaactt
     5221 ttggaagaac aaattggcgt gtacgagacc gtgaaggggt cattaataga ggaagaacca
     5281 gaaaaagagg gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg
     5341 tatgatattg attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca
     5401 gatgcacgag atgaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa
     5461 gaagtttacc gacaaatttt aaatgcattt gatgtgggga ctttagatag caatgaagat
     5521 atttttgtca ttaaacgtcg ctattttaca caagcaagtg atgaattgat tcatgccttt
     5581 tccaatgagt ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca
     5641 ggtgaagatc caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat
     5701 aaagccaaac atccaaaagc gaagccattt aaatacccac aagaaatgaa gcgagcctgg
     5761 cgtaaaatgt tagatgatga gttgattcca cttgagaatg aattgaggta aacgagaaaa
     5821 ttgaagaaat tgtatgataa acatgttaat aaatgatgct cgaaataaaa tttggtttta
     5881 tttttacatt gatacggtaa ataaaatttg aaagtagaag aaaatatgaa caacaatgaa
     5941 acaaaaaaaa catatgaact aagaataact gctgatgaga ttaaatcaac agacccaaca
     6001 tttactatga agaatctaag cttaggggat aattatcaac caaaagatgc ttacttccgt
     6061 acggcagaag gcgaaataac tagaagaaac tacggtaaaa atgaacggaa aatttcaaat
     6121 caaacattac cgcgattagc tattcaaatg tttgaacaat cgattgtttc tctaccagaa
     6181 agcgaaagag caacatttcc tgtgtgtcga tatcactcag gagatgagat aatacgaggg
     6241 atttttccag atgttgacag tttcaaaaaa aaaaggaagt ccattgaatt tttgatttat
     6301 aaatataatg aagacaagca gatagtcatc tattgttgga gtattttttc aactttatta
     6361 tttgtacaag aatgtttgaa acgttttgga acatctgggg atgaatttat tcttgtttat
     6421 aaagggaaag agaaaaagga aatacgagta ggagagactc agactatgac caatcatttg
     6481 ctgaaatcat atgttttaca tttactcaaa tctaaaaacc ttattctacg tggcgctcct
     6541 ggaacaggga aaacattcct tgcaaatgaa atggcagctg atgtcgtttc aaatggcaga
     6601 actacacaaa tcaatgaatt gagcgaagat gaaaagagcc gaattggatt tgtccagttt
     6661 catcctagct atgattatac tgattttgtc gaaggtttac gtccaactac atccgatgat
     6721 ggcgttgtta gtttcacctt gaaagatgga agctttaaga gctttgttga aaaagcaaag
     6781 aaaacaaaga aagtaaatgg acaagataat tttgaagaag cttgggctaa atttttcgat
     6841 gcggtaacgg aagctagtat agagggagaa ggctacaata aacttaaaac attgacaggt
     6901 aaaccagtac gcaatcttct agcctatgaa agaaatgaaa ttcaaggtgt ttatcctgct
     6961 aaaaccaata cttatctaaa ccatgaccaa atttataatg tctatcgtgg attgccaggt
     7021 acgccaaaag gtggatttga tgcttatcga aaagcgattg taaaacattt aaaagaagaa
     7081 tttggcttaa aaaactacac ggcgtcaacg gtagattcta cgaataacca aaactatgtg
     7141 tttatcattg atgaaattaa tcggggcgaa atttccaaaa tttttggcga attatttttc
     7201 tcaattgatc caggatatcg tgggcataaa gaaggtgttt acactcagta tgcaaacctc
     7261 catgaaaatc cagaagaaaa attctatatt cctaataatg tttatattat tgggacgatg
     7321 aacgacattg accgttctgt tgacactttt gattttgcta tgcgccggcg atttactttc
     7381 ttagaaataa aagcagaaga ttcagcacaa aatatgctga ctcaagagaa aacaaaagaa
     7441 cttatgacgc gtttaaatga ggcgatcgtt gccaaagaaa aaggtggatt gtcaactgat
     7501 taccaaattg gtgctagtta ctttttaagt atcgataatg gtgaggagac aatggaacaa
     7561 ttatgggata ctaagttata tccattattg aaagactatt ttcatggtga acataaggcg
     7621 aaagaaaaat tagaaatact agaaaaggta tactctaatg acggtgattt agatgaagct
     7681 gaaacctcta cacgataatc aagaaatcaa tgactcagtt gtgcaacatc caatcattac
     7741 gaacttagtt aatcgtgact taagtagttt agagaaagaa aattttatta tctttccgca
     7801 acagttaaat caatccaagg atctaagtga agataacttg atatttcaat caagaaatgg
     7861 aaaaatttgg acttgtaatg tcgtaggtat ccttagcgat tcaacagatg aattaagaat
     7921 tcattctaga ttcgctaatc aaatgaagca tgaagatttc tttttacgtt atatgcttca
     7981 gcgtgttttg aattacaacg tgattaatga tcattttagt gcatctaaaa agatgacata
     8041 ctacgatttg ttggtatttc tctttcctta ttatctgaat gaagcaatgc gtaaagggac
     8101 atacaaagaa tatgtcaaac gagaatacaa taatgccaat atcaagggag ctgttgatgt
     8161 tgcaaaacac atccgttcga atgttccttt cgttggaaaa gttgcttatc gaacacgaga
     8221 gtttagctat aataatcacc taacccagct tattcgtcat actattgaaa aaatccaaaa
     8281 tgagtacgat ttcttactta gtggagatga ggatacgaaa gaaaacgtgg ttctaatcaa
     8341 acaaacaaca cctggttatg ctagattaaa tcaatttaat atccttcaag agaatatcct
     8401 tcatccagtc aaacatagtt attacgagga gtattcggct cttcaacaaa tctgtatcca
     8461 aattttatcg gaagaaaaat caggatttgg aagtgataaa aatcaaatac atggaatcat
     8521 tatcgatgtg tcttggctat gggaagaata cattggaaaa gtcactggtt ggaaacatta
     8581 tggcagagat aaagggttag caacaatgca tttatttcaa gagcccaacc ggtccccacg
     8641 atatccagat tttacattta ataatatacc aattgataca aaatacaaaa aacatcttga
     8701 tactagaaat gactataatc agttagtcac atatattcat ataatgaatt tagaccaagc
     8761 agatacaatg aaaggcggct ttctgcaacc tacttccgat ccccttcctg caaatcatgg
     8821 gtat
//
LOCUS       DACEJR010000098         8732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEJR010000098 DACEJR010000000
VERSION     DACEJR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.101"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..297
                     /locus_tag="IW651_002597"
     CDS             <1..297
                     /locus_tag="IW651_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815277.1"
                     /translation="ERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLL
                     SLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(423..1100)
                     /gene="nth"
                     /locus_tag="IW651_002598"
     CDS             complement(423..1100)
                     /gene="nth"
                     /locus_tag="IW651_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0815278.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1107..1805)
                     /locus_tag="IW651_002599"
     CDS             complement(1107..1805)
                     /locus_tag="IW651_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0815279.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2073..3257
                     /locus_tag="IW651_002600"
     CDS             2073..3257
                     /locus_tag="IW651_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0815280.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3263..3706)
                     /locus_tag="IW651_002601"
     CDS             complement(3263..3706)
                     /locus_tag="IW651_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0815281.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3932..4219)
                     /locus_tag="IW651_002602"
     CDS             complement(3932..4219)
                     /locus_tag="IW651_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815282.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4390..5742)
                     /locus_tag="IW651_002603"
     CDS             complement(4390..5742)
                     /locus_tag="IW651_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0815283.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6015..6461)
                     /locus_tag="IW651_002604"
     CDS             complement(6015..6461)
                     /locus_tag="IW651_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0815284.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6697..7623)
                     /gene="cysK"
                     /locus_tag="IW651_002605"
     CDS             complement(6697..7623)
                     /gene="cysK"
                     /locus_tag="IW651_002605"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0815285.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7763..8650)
                     /locus_tag="IW651_002606"
     CDS             complement(7763..8650)
                     /locus_tag="IW651_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0815286.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2379 a         1784 c         1443 g         3126 t
ORIGIN      
        1 gaaagaagct ctcatgcaat gattctcact tttttatggt tgttgttcac cagaatctat
       61 atttttctga tgagcctgaa accgcccaaa tccattagtt tatgtttagt tataaatggt
      121 atttttttat tatcacttat catttttatt acacctttag atagatacgt cacttattta
      181 tataatcctt tggaatactg gacttatttt ataggtatat tggaaggcat tattttctac
      241 atcgggtatt ttcccaataa aaataacaac ttttattttt atcgcaataa attgtaataa
      301 gttaccatcc tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa
      361 acagcttttc tccataataa caagataatt catacattta tttgcaatta aagaaaccac
      421 gtctacaggc gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca
      481 tcttgacata ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga
      541 tatctaccaa aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc
      601 cgcatcagtg tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt
      661 tttgagacac gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc
      721 aatacgacat ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac
      781 gttggcactt gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt
      841 gctttatttc gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct
      901 tctgctaacg catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact
      961 gatacatctg tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg
     1021 tgcttgagtt ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt
     1081 gttttttgtt tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc
     1141 ggcaattctt cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt
     1201 cgtttttggt cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc
     1261 aaaatgcggt caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct
     1321 agcttcaaaa tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac
     1381 tcgatcgaag aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt
     1441 tctttttctt gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat
     1501 agatcataga tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt
     1561 tctatcttga taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt
     1621 ttgattccca tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt
     1681 gccatatgca actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa
     1741 tttttaagta aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc
     1801 aacatgattt ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac
     1861 aggtttcttt tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt
     1921 ttgtttgttc atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat
     1981 ttcgctgttc cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc
     2041 aagttaacat tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta
     2101 aagtttttgg atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg
     2161 tacttccgtt tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca
     2221 ctttgctcat gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct
     2281 taagtgatca ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg
     2341 gatatttgct ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg
     2401 aaggattgac ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac
     2461 ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc
     2521 tgggtcctat tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg
     2581 gagcactctt tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc
     2641 caataaaaaa acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac
     2701 aacaattatt caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt
     2761 tggcagctgg ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga
     2821 aggctgggtt ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat
     2881 tgttcattat gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga
     2941 tgttcagtga aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga
     3001 ttttgttttt atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat
     3061 tcaatggcca actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta
     3121 cccaaagtat acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg
     3181 ctgcagcacc atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc
     3241 actacaataa aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga
     3301 taaaacgaaa tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc
     3361 gctgtaccca tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg
     3421 tcaattgaaa atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc
     3481 tagctatcag tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg
     3541 ctaattttgt cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat
     3601 ttagagtaaa tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat
     3661 tagctaaatc cagatattct tctattagca gttgtctttt atccatttct acaccttctt
     3721 tttctttcat tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg
     3781 aaaacccaat ctgaaaccaa gaaaaaaagg ggttgacaaa gcttgtcata accccttttt
     3841 ctgattaaaa gatagtaaag agacagtcat cggtaattgt gatgattttt tgtcatcact
     3901 taaggatgag cggccttttc aaaaactgtc tttaaagatc atcatcagtt tctagcacat
     3961 atggctgacc ttcatatgtg tactctgtat tcaatgtgcc gtcattttca tctgagtaga
     4021 tgcgtctact gaaaggaaaa cgatgagaaa cattatcttt ggcgtcattt gctggcatcg
     4081 tgtaatattc ctcttgcgtt tcattcaact gagagagtac ttgatctgcc aactcatcaa
     4141 tattgatcgt taatttcttc gaagcacttt tataatcttc taaaaatgca tcttgttgga
     4201 caaaatattc tttattcatc ttctttctca cctttctttt caaaatcaaa taaagctgct
     4261 tgttctttta tgtaacagct ttatctttgc tgcttattgt gacaaaaaat tcgaagaaag
     4321 acaactaatt gtacttttca caaaaaaaga cagagggtcg tgacaaaatt gtgtcacaac
     4381 cctttttctt tattgtgcag tttcgatttc gcctagcgct gcttgtgcaa gtaggttcaa
     4441 gtaattccat gggcggtcaa aatgtggttg gaagaagaaa tctgaaagtg cgagatcttc
     4501 tacagtcatt ttattttgaa cagctaaaga catggtattt gctgattgtg taatgtcgta
     4561 tttggacatg aactgagcac ctacgatacg attcgttcct ttttcataga ctagctccat
     4621 cattactttt tcagtagttg gcatgaactc tggacgataa ttatcttcaa atacagtcgc
     4681 ttggacttct agatcgttca aagttgcgct ttctttggtc acacctgtcg aaccgatcgt
     4741 ccagccaaat aggtaaagtc ctgaagttcc ttgagtacca cgatacgcca ttttatgatc
     4801 tgtcaagtta tttcccacaa gcaaaccttg acgaacggca tttgttgcca aaggaatata
     4861 gttttgcgtg ccacttggat tgtagttgac tactgcagag tctccagctg caaaaatgtc
     4921 tggattactt gtctgcatgt attcattcac tttgattgcc ccgtttggca gtgtatctac
     4981 ttttccttcg atcagttttg tatttggacg gaagcccaca cataaaatga ccatatctgt
     5041 atcaaatgta tctgttggtg tagctacttg tttcactttt ccttcttcat ctgtgataaa
     5101 ttcagtaaca ttttcgccta gcgcaagagt aacaccgcgg tctgtcaatt ctttttctaa
     5161 gatatctgtg aatggtttat ctagatattt attcaagatg cgatctaatc catcaatcaa
     5221 tgtcacttgt tttccagatt cgacaaaagc ttctactaat tcaataccga tatatccacc
     5281 accaacaatt actacacgtt ttgcttcttt cgcttgtgca ataatttcat ttgcttgatt
     5341 gtagtttttg cataggagta catttttcgc atcgattcca ctaatcggtg ggataatagg
     5401 ccatgaacca gttgtcatca ctaatttatc ataagaaacc gtttctgttt ctcctgtttt
     5461 cagatctttt gccgttactg ttttattttc tgtatcgacg tctgttacat cgtgttccat
     5521 tttaactgtt gctcctaatg aagataattc ttcaggattt gagtagaaca agcctgctgg
     5581 gtcctttact actccgccga cataaagtgc gataccgcaa gataagaaag aaatattgtc
     5641 atttctttca tatactgtca cttctgcttc tggatgattt tttaaaatac tttttacagc
     5701 tgcagtacct gcatgagtac agccaattac tactactttc atttatggtc cctccgatta
     5761 acattttgtc cttcgacaaa actactataa cagaaataaa acggatacaa cagtttaatg
     5821 cttgtgaatt cacaaaggtt tgtttgtagt ttcattcctt ttcaagcata agaacccaat
     5881 atctacaaga ttcttatttt aataatattc acttttatat ctaagattgt gaataaaaga
     5941 caaaaaaata aaaaagctag cgtttttttt acacagctag ctttttttag gtataacact
     6001 tgtagaaact gtttttacat ttgcgccttt tttgctgact ctttacaatc cggacagagt
     6061 ccatacaatt ccaaccgatg ttcattaatc tcaaaatcag tcagtctgct tgcagccatt
     6121 tctatatctt caagccctgg gtaatgaaaa tcaacaatct taccgcagtt ttcacaaatt
     6181 acatgataat gacgctttga actgaaatca aatcggctag aagcatcgcc gtacttcatt
     6241 tcttgaacaa atccaatcga agtaaataag cgaaggttat tgtaaacagt cgcaacactc
     6301 atatttggaa accgatgttc aagtgcttta taaatctcat cagccgttgg atgggaatga
     6361 tgttcaatca aatattctaa aaccgcgtac cgttgaggtg tgattcgaat gttctctttt
     6421 tttaactttt cgataacctc ttcgactaac ttattttcca tgtaaacacc ccacgttgtt
     6481 attctcgtct tatcatacta tttttacatt ttaaaatcca cttaacatga aaagaaaatt
     6541 caattttatt gtattttaat gaaaaaagac gtaaaacagt cgcgttcact attctacgtc
     6601 tctattcaaa taatttttct catcataaat ttttgtgtgt tcttttcatc tttaaaagtt
     6661 ttttacataa acctatctgg agaaaaacca agtgccttat atctcttggt aaagggctgt
     6721 agataaataa cgttcaccat tgtcgggaac cacagctaat accttcttgc cttttcctaa
     6781 ttcttttgcg acttttagcg cggcagcaat tgcagcgcca gatgagatgc ctactaaaat
     6841 cccttctttt cgcccaactt ctctagctgt ttccatcgct tcgtctccac taatagaaag
     6901 gactttatca tacacagaag tatccagtgt tttaggaaca aaacctgtac cgattccttg
     6961 gattttgtga ggtcccgctt ctttcccttc taaaatagca gattctgctg gttctacccc
     7021 gattaattcg attttcggat acacacgttt taattctcgg ccagcgccag tgatcgttcc
     7081 tcctgtaccg atacctgcaa caaacgcgtc caagccattt actccaaatg cttgatgtat
     7141 ctcaggacca gtcgtcttct catgcacaag aggatttgct tcattttcaa attgtaatgg
     7201 taaaaagtag ccgttttctt ttgccaaacg ttctgcttct ctgattgatc cagaaatacc
     7261 atccgcacca ggagtcaaaa tcaactctgc gccgtaccct ttcatcaata aacgtctttc
     7321 gatactcatc gtctcaggca tcacgatgat tactttatat ccttttgcaa ctcctaccat
     7381 cgacaatccg attcctgtat ttccagaagt aggttcaatg atagtatcac ctggtttcag
     7441 tagaccatcg tgttctgctt tttctatcat acttaaagca atacgatctt ttacacttcc
     7501 tcctggatta aaaaactcaa gctttacaaa aacatctgct gaatcttctg gtacgatttt
     7561 atttaatttg acgattggcg ttttgccaat caattcggtg actgagttat agatagctgt
     7621 catcttcaat tcctccttgt ctttgctctt cttacttatc ataatacaat ctgttcattt
     7681 atggcgagtg attcgtcttt gactagcaaa aaaaagacaa agatatacca tatatggtaa
     7741 tttctttgcc tttttatcta aatcaaacgt ttagttcttg accaactaat aaagaataac
     7801 tggacataga catgccattt ttcgccatta gatcatcaac agacatgcca aatttttgtg
     7861 caatatccca tagcgtatca cctgacacga ctgtatatgt ctgaccatta tttgtattta
     7921 catctgttgt tgaagatgat gacgttgaag tcgtcgtagt agtcgttgtt gttgtacttg
     7981 tgattgttgt agccgaacct gttgatggcg tatcaaaacg ggttaaatta taagtattaa
     8041 tcaatgagtt caatttggcc ccataatttg gatcggtagc ataacgacct gtcaagtacg
     8101 ctgttgcatc ataaaaactt gttgtattgc ttttccacac gccagcataa tgataatcgc
     8161 ccgtggaaaa actggttgaa cgaagaacat tcgcatggtc ttggaaggat tcccaataag
     8221 aagaatattt tcggaatggc tcattcatcg ttacccattg tccgtttaca tattctttgg
     8281 ttgccatata aacgacctgt ccgttatagc ttcctttcac accgaataga ttataatttg
     8341 gcgcactagc taatccggaa gtgccatatg cactttccaa caacgcttga gcgatcatga
     8401 ctgaagcata caagtcatta cttgcagcaa cttctgacgc agaccagccg atttgatcga
     8461 taaacgcttg aggatcagcg actgtttgag atccttgttc atctgcatga acatgttgcg
     8521 gtaccgctgt aaccatcatc ggcccaacta aaagactcgt tccaattaaa ggagcaatcg
     8581 cttttttatt gatcaccatt tttcttttag gtctatggtg ccgggcactg cgggtcttca
     8641 gctcttgcat tcaaattcct cctcaaaata aaaaaactta ataaaaagtt ctcttcttaa
     8701 aacaaactta attaattaaa ttatactttt aa
//
LOCUS       DACEJR010000099         8413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEJR010000099 DACEJR010000000
VERSION     DACEJR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.102"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            340..1779
                     /locus_tag="IW651_002607"
     CDS             340..1779
                     /locus_tag="IW651_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0815287.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2074..2292
                     /locus_tag="IW651_002608"
                     /pseudo
     CDS             2074..2292
                     /locus_tag="IW651_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2541..2756
                     /locus_tag="IW651_002609"
     CDS             2541..2756
                     /locus_tag="IW651_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815288.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2973..3229)
                     /locus_tag="IW651_002610"
                     /pseudo
     CDS             complement(2973..3229)
                     /locus_tag="IW651_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3416..4327)
                     /locus_tag="IW651_002611"
     CDS             complement(3416..4327)
                     /locus_tag="IW651_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0815289.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4346..5350)
                     /locus_tag="IW651_002612"
     CDS             complement(4346..5350)
                     /locus_tag="IW651_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0815290.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5347..7470)
                     /locus_tag="IW651_002613"
     CDS             complement(5347..7470)
                     /locus_tag="IW651_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0815291.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7475..8395)
                     /locus_tag="IW651_002614"
     CDS             complement(7475..8395)
                     /locus_tag="IW651_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0815292.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2414 a         1716 c         1554 g         2729 t
ORIGIN      
        1 aaagcaaatt ggaaaacaaa taggtgcaac accataaata tgaaaaaatt gggaaaaagt
       61 agaatggaaa tttgcttacg tctcacgaga tagctcacac tattaatctg aagaacaatg
      121 aagtcgactt ttagaaattg ctagaagaaa gtaaagcgaa ttatcactag ttactataga
      181 gtaagttcaa ataaagcatc tccaacttac atggttacat gaggatatca aatcgaaatt
      241 actcaagctt cctatggcta gagtcgcgag aagttgttgg gcagtagaca taggttatat
      301 tgttcttagc tacacgatca ttagaaagga aatcctatta tgtatagtta tcaagaagat
      361 gcaaaagtcc aaaaaaatcg ctggtggatt ttgattgcta tatctatgtt taccttcatg
      421 tccacattgg atggcagtat tgtcaatatt gcactaccta ccatctcaga tgatatgggc
      481 ataccaatga atcaagcgga atgggtagtg tccgtttatc tgatgattgt ctgttcttgt
      541 ttgttgctgt ttggtaaaat tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt
      601 acgctgatct ttgtcttggg atcgctgttg tgtgggttca atcattcgct aggtatttta
      661 ttgttcgcac gggtggtaca agccattggt gctagtatga ctatggctac aaatgccgga
      721 atcatcacgg aagtctttcc catacatgaa cgtggtcggg cattgggagc aataggtgcg
      781 tttgtttcat taggctcgat tgctggacca ggtcttggcg gcttgatttt gtcccaattt
      841 tcttgggcgt acattttttg gatcaatgta ccggtgggaa tcgtaacgat gttgattgct
      901 gaaaaattct taccaaaaga tatcaccatg agcggccata caatcgatgg agggggcttt
      961 gctgccattg cggctacgat tatcagcttt tttggagcaa tctttttggg gcaagaaagt
     1021 ggctttttcc atccagtccc ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc
     1081 tttttaaaag ttgaaaagaa agcagcctct ccattgattt ctttcggtat ttttaagaac
     1141 aagattttta ccatgagctt gattacagcc ttgttgattt tttcttccaa cttctttgtc
     1201 aatgtagtga ttccttttta tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg
     1261 ctgttgatga tggtctttcc tttgatgatg gtcgtcgggg cgcctttaag cggctacctg
     1321 accgaccggg ttggcccgga gattttggta ttagtaggct tgggcttatt gtcggtaacc
     1381 caggtcatgt atatgttttt gtcagtggac tctcctttgt ggctatacat tgtcgctact
     1441 gccatcatgg gtgccggcaa tgccttgttc cagtccccca ataatacgat ggttatgagc
     1501 tcggtcacta tcgaaaactt gggactggca ggctcgatga attcctttgc ccgaaatttg
     1561 gggatggtca gcgggatttc gctttctacg acggtcttat atcaagcgat gtctgctaag
     1621 atgggccagc aagtaactaa ctatatcgca aatcggccgg atgtcttttt atatggaatg
     1681 aagattacct ttttaggcag ctttgttttg tgttttgttg cctttttcct gactatctgg
     1741 cgtatcaaaa aacaaggagg tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt
     1801 tttggaggcc tgcgtatggg tgagtgggat ttccatggat gatggaagat gaagtgaact
     1861 tctaaaagcc gaaaaactca agtattatct tcaaaatact agtgctcaat gagatgcttg
     1921 atttgatcac aagaagactg cgaaagccgc tggcaaccgc aagctaggta tcccaccgat
     1981 ggactacgtt ttagaggcta ctggttatga atacggagcc aacgctctca tctgcataag
     2041 aatacatcac ccagattatg taaagatttt cgatacttct gtcaaaaaat cattaagata
     2101 gataaatgat ttccgtctag tcaaattggt tcagaatgta gacacacaga tggtaagaag
     2161 tcactcacaa ttcgagaatg gacttgtcct atttgccgta tacatcataa tcgaaacgtc
     2221 aacgatagta tcaatatttt gaccaagggc tatgaataca agcattgcct tagaaaacat
     2281 caagaacctt aggaacaacg gggatagctt ggttaataag agataccgct gtttaggctt
     2341 gcaagtatac tcttttatca agaattccgt ggctttagtc atgagaggct taatactatg
     2401 aaaattttct ttagcatttt tgatttaggt tccttttcta attataataa gatgaccact
     2461 acagtaacct actgaaatat ataaacagcc actgtagtac cattttcaaa gtagtctatg
     2521 agaggagaat cttcattctt atgaacgctg gaattgcgga tatgaattta ataaaaaaaa
     2581 cccttaatga ttttacttcc aattcaatat caaaaggtac tggtatcaat ttaagcacaa
     2641 taaaaaagct gaaaagtggt gaaagatcag tggaaaagct gaatctttta gatgctataa
     2701 aaataacgga atttgctatg aaaaatggaa aagctgagat tgaaatatgg agataattat
     2761 cgtctagtga attacttgcc acttagctga atttaacgat ccttggtgct tgaaggggga
     2821 gattcttggg aatagagcat acttgcaagc ctatgtcttt actaacaacg atatctctta
     2881 ttaaccaagc tatccccgta gttctaaggc ttttactttt atctaagcaa taatcgtatt
     2941 caaaggaaat ttttgaccct tagaaagtct cattttatca gacgtgagct taggcattgc
     3001 tgaattttgt taattcggtt ttaggttgtg ctttgagcat aatatgaaag tattctttat
     3061 cacgatttca ttcatctaag gtaatgtgat gtgattctga aattctttca aaggcagttt
     3121 ttgcataagc tgacattaca tcatcaatga catgtttaca atgtttcact actaaaataa
     3181 gggggtaacc aagaagaaaa actgaatgat tatttgtatc taattccatg gtatatcaac
     3241 catttcctat atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt
     3301 gtcggttttc gagttaccga atggcattca tcacccacct atagaggatg ggcgacttct
     3361 gcctgaaata agttacacta cttataactt ctaataaaat tagattgagt cattattatt
     3421 taacatcaac gattttccag tttccatttt tttgtaatac taatttgtac tgaaagtagc
     3481 tagtggtttt tgatacatta tcaaggtatt ttatctttgt actcactaga aaattttttc
     3541 cgttttgtag ataaactgga ttctctaact ccgaaaactt taagtcaata ttgattggtc
     3601 gcatcgcacc attttcaaca taatactcca attctttttc agaagctgat ggataaagtt
     3661 taaaaaatgt ttctaaaaaa tcggtgattt cttccgttgt tttggcatca atttctgaat
     3721 cgttttctac ttgttttgct tcatattttg ccttgtctgg ctttccaatc attgttggca
     3781 aactagttac taaatgattc ctgttttcat cttcaaagat tgtcactcga tatgcagaag
     3841 aaattgattg cgtctttttt ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa
     3901 attcttccga gtgtttttca acatctagaa tttttacaga ttgaacttcg gaactcgtgg
     3961 gaatatctgc tcgaaccata tcttggatta aagcgagacc gttttctgta aggtactgtt
     4021 cgagattact cattctcttt tcaattgctt ctttgttatt tttccatgaa aaatattctt
     4081 tcgcaaaatc ctctgtaaaa ctttcaacgc cagaagtatt cactaccttc tcttttataa
     4141 ccactttttc atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag
     4201 caagactaca agacaataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg
     4261 atacaactcg ttcttttttc ttttttgggc tcttttcctt ttgattccgt tcaattttta
     4321 tcttcattct ttttcctctt ttctcttatt tgtatcggcc agctccgata agatgctgcc
     4381 gccaatagtt ttccgttaga ttcgtccacc ccactggatc tcctgcatga aacatgcgat
     4441 tctcccctgc gtaaatgccc acatgggtca cataagtacc ggcatcatag gttgaatgaa
     4501 agaaaactaa atcacctggt ttggcatctt ttagatcaat atgagtcatg gcatcatatt
     4561 gatcctgagc cacccgagga agtttcaggt caactttggc ataacaccat tgggtcaagc
     4621 cactacaatc aaaccccgtt tctggacttg agccaccaaa cacataggcc gttccttcat
     4681 acttaaatgc ctcatcaaaa atttcttgga ctattttgtc atcaaactga gttgtagtta
     4741 ggtattgctt cactaataaa cagtaaaaca tattgccata attataccgc cacccaccat
     4801 tttccttaat cgcgattggg ttggggtaat cgactttttg accacctgat ttttcctttg
     4861 aaaaagattc agctaaagca aaactatgct tcttcccgtt tttcccgaca tgatctagat
     4921 acccgccacc atagttatag gcctgaataa tactatcttg atcacaccct agttctttgg
     4981 aacgcttaac caaagacgca aaatagagac agccttgttt gatggatgcc tcggtactta
     5041 atgaattagg tggtaatccg gctgactcag aactttgcat aacgtcttcc attgtgccac
     5101 cagattccac ttgaataatc gccaaaagaa ttggaatata ctctgttata tccgattctt
     5161 tggcatactt ttccaccatt gcacggtgtt tcaatacttc ttctgagaca ctagttccgc
     5221 caggagaagt gccagaacta ccatttccct cactgtcttc accaaacaac aaacccacac
     5281 acatcaaaat acccataaaa gagatggtca gaaaagttaa tgaaataaca cccactttct
     5341 tcaagttcat tttttctcac ctgaccttga ttttggacga tggttcattc ttttttgtac
     5401 tttttgattg gtctgctttg gcagagtgga cctctgtttc acttgttgga aagaattttt
     5461 cttacttgct gattggcttt tcccttttga aatgatttct tgactagtta gttttcgttt
     5521 gatttcggga ttcagatttg ctctcacaat ccgctttggt gcttcttttt tcagcatcat
     5581 atctttctga ggttgagctg ctttatctat tggaatattc gcccttcttt ttccctgacg
     5641 atttgttgct tcactcagta tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg
     5701 ttgtttttct cgcaaatcca ctcgatccgc tttttcttgg aacagtccgc gcttgaattc
     5761 ttctggtgct tttttcgact tttcgattcc tttatggaga ttgtatttca aattcgttgg
     5821 cgtattcctt acctgatcct ttacttgttt agtcttatct ttcaccaaag cttgggtatc
     5881 caaaactttg ccgatttttc tcccagccaa attcattcgg gattgtttct tattatttga
     5941 aactggattc tccttagtta agtcagatag tatttcgtga tcgttcggta atcgtgaatt
     6001 ctttcgtaga gaagaaccgg atggcttaaa tttggatttt gattgttcct ttgccaactt
     6061 atttccgact gctgcaccac caccaaaagc caaagctcgt cccacatttc gattgagttg
     6121 ccgcaatttt cgattcatta acatttgcgg ttttcgcatg acgcgtcttc ccattgactg
     6181 gctatcatta ctctggaggt tgaacatact cataatgtcg cccaatttga aatagatacc
     6241 ggcaaaggtc acaatttgca gaaaagcaac catgaaaaat ggataggttg ccgaaatatt
     6301 gaaaaacatc gtggagatgc taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat
     6361 cataatcgta ttgaataaac gaataatcgc cttctttcct aaactttcat aagtcggcaa
     6421 catggacaat aagaaactaa ttggcagaaa catcgcaaag ataatgaaga gaatttgcga
     6481 gaacaacata atccctgtta gaagaaaaac aaagaaggaa atgccaatat tgaacaatac
     6541 caaaaagacc acggtcccta aacggttcat ggtcttggta atactgagat tcgcattgtc
     6601 attgtcttca atctctgcct tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg
     6661 actcacagat agaatcttgt tgacccggtc ttcaccaatt tcttctatgt tggaatcatc
     6721 aaattgcagt agtaaccaag gttgttggac ttgaatcgaa aacaaactat cccgaatcaa
     6781 atccacacta tcttttcctt ggctttctga attgggtaca acgatctcgg ttccaagggt
     6841 aagggccgct tcactaacat ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc
     6901 ataagcaatg aacgaacccg atagtagaaa aatcatcaca aaattaatca cggctcgaac
     6961 tgctttcgtg gtttctcgtt ttaataatcc ggtatacgcc acatacaccc ccagagctaa
     7021 aatcatcaat aatagaaacc caaaataaaa tccggaggtc tggagtccat tttcagtaat
     7081 accagccaat gtttggatat tctttccaat actttcagcc gtatccgaaa tgaaatctaa
     7141 tttgtaagcc tcttgaacca cataacccgt ggcgttcgac aaatacaaac tcaccgtcca
     7201 aatgaaatta gtaatggcat aaagaccata catcacgctt ttccctaagc catcacccca
     7261 attccaaggc aaccaatccc aagagctatc cacaaagaaa tctagttgat agttgttcag
     7321 tgaatatttg gaatatagat ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc
     7381 ggctaccgta ccaactaaac tcaatagaac tatcagagtg agtatgctaa taaaaacgat
     7441 tcccacaatc cgaaaaagtt tctttttcat cccctcactc cattctctct tgaacaggtg
     7501 gacgggtatc aaaggcatgg aagagttctt caaagactgg atgtacctga accacgccta
     7561 cccgaccata caaatcctgc atcaagcatt ggccattttc caaatctcgt aggcgttttt
     7621 gattgctttc atcttctcga tccaagccaa agaattccaa ggttttcttg atttcatgaa
     7681 tatcggtact gcgaaacgca aacttcaacc cgatattatt cttcaagcgt tcatccagta
     7741 aatcatcact attttgcgtg acaaagtaaa ccccggcatt catagcccgc ccagcacgaa
     7801 cgagcttatt cgataatgtt ttcccttgcg ctacttgaag aaaactccac gcctcatcta
     7861 agtctacaat tttgaagatt tctcgattac tatggataaa gtccaaggca aaagtggaaa
     7921 tgacgattag catggaaaca ctaagcaatt ccatggtggt atattcttca aaagtggttt
     7981 ctgcatctgg taaaaccaaa tccgctactt gaatgatatt taactgccta tctaaactaa
     8041 tcgactgggt aatcgaacca tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg
     8101 actcgatgtg atccgcgata ttttccgcaa ctggtgagcc ttcttttcga agctcattaa
     8161 ttacttgtaa taatccccgt tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca
     8221 ctggaaactt ctccccatct cgactagaaa ttcctgttaa aaaggtcaga atatcaattg
     8281 ctagactttc agaatccttc tttttctcca taatcacata aggatcaagt aatccttgat
     8341 tttttggctt actagttaag ttgagaatat tgatttcatc cgcaatttct ggcaaggttt
     8401 ctttccagcc acc
//
LOCUS       DACEJR010000100         8368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEJR010000100 DACEJR010000000
VERSION     DACEJR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.103"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            341..766
                     /gene="lacA"
                     /locus_tag="IW651_002615"
     CDS             341..766
                     /gene="lacA"
                     /locus_tag="IW651_002615"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0815293.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            783..1298
                     /gene="lacB"
                     /locus_tag="IW651_002616"
     CDS             783..1298
                     /gene="lacB"
                     /locus_tag="IW651_002616"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0815294.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1309..2241
                     /gene="lacC"
                     /locus_tag="IW651_002617"
     CDS             1309..2241
                     /gene="lacC"
                     /locus_tag="IW651_002617"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0815295.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2244..3224
                     /gene="lacD"
                     /locus_tag="IW651_002618"
     CDS             2244..3224
                     /gene="lacD"
                     /locus_tag="IW651_002618"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0815296.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3252..3569
                     /locus_tag="IW651_002619"
     CDS             3252..3569
                     /locus_tag="IW651_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0815297.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3569..5275
                     /locus_tag="IW651_002620"
     CDS             3569..5275
                     /locus_tag="IW651_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0815298.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5417..6823
                     /gene="lacG"
                     /locus_tag="IW651_002621"
     CDS             5417..6823
                     /gene="lacG"
                     /locus_tag="IW651_002621"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0815299.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6846..7118
                     /locus_tag="IW651_002622"
     CDS             6846..7118
                     /locus_tag="IW651_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ0815300.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            7139..8038
                     /locus_tag="IW651_002623"
     CDS             7139..8038
                     /locus_tag="IW651_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0815301.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2655 a         1423 c         1721 g         2569 t
ORIGIN      
        1 attagcttag gtcagatttt gtataagacg aaaataaagt aggacctctt aatcagtaag
       61 ttatagaaag taaaagactt ttgtaatact tgaatagata tttcacgtcc attttgtgat
      121 ggattaaatg aacaaaaatg aacaataatt taacggtgtt atctattttt taaaaaaaca
      181 aataaaaaaa acaaaaaata aacaaaaaaa gtgttgcttt ttgtttgaat gcttgatatt
      241 atataaacaa agaaatgatg aaaacgtttt ttcgaacatt ttgcaaaatg ttttctactt
      301 ctacgtagca tttctttcta aaatttagga ggtggtcaaa atggctattg ttgttggtgc
      361 agatctcaaa ggtacgagat taaaagatgt tgtgaaaaat tttcttgtag aagaaggttt
      421 tgaggtaatt gatgtgacta aggacggaca agattttgtt gatgttacct tggcagttgc
      481 ctctgaagta aataaagatg agcaaaactt aggtattgta attgatgctt atggagctgg
      541 tccattcatg gtagcaacta aaattaaagg tatggttgca gcagaagttt cagatgaacg
      601 ctcagcttat atgacacgtg gacataacaa ttcacgaatg attacggttg gcgctgaaat
      661 cgtcggtgat gagcttgcta aaaacatcgc taaaggtttc gtcaatggta aatatgatgg
      721 cggacgtcat caaatccgag tagatatgct taacaaaatg tgttaagaaa aggagttaag
      781 gaatgagaat cgcaattgga tgtgaccaca ttgtcacaga tgaaaaaatg gcagtatcag
      841 aattcttgaa atcaaaagga catgaggtcc ttgatttcgg gacatatgac cacgcacgta
      901 ctcactaccc tatctatggt aaaaaagtcg gtgaagcagt agtaagtggt caagcagact
      961 tgggagtatg tatctgtggt acaggtgtcg gcattaacaa tgcagtcaat aaagttccag
     1021 gtgttcgctc agcgttggtt cgtgatatga catcagctat atatgcaaaa gaagagttaa
     1081 atgcgaatgt tatcggtttc ggtggaaaga ttactggtgg tcttcttatg aatgacatca
     1141 ttgaagcttt cattgaagct gagtacaaac caacagaaga aaataaaaaa ttgattgcaa
     1201 aaatcgaaca cgttgaaaca cataatgcac atcaagcaga tgaagatttc tttacagaat
     1261 tccttgaaag atgggaccgt ggtgactacc acgattaagg tataacaaat gattctgaca
     1321 gtcacactaa atccttcagt agacatctct tatcctctag aaacattgaa aattgataca
     1381 gtgaataggg tgaaagatgt tagtaagaca gctggtggaa aaggcttaaa tgttactaga
     1441 gtcttgtatg aatctggtga taaagttact gctacaggtt tcttaggtgg taaaattggt
     1501 gaatttattg aaagcgaatt agaacaatct cctgttagtc cagcttttta taaaatctca
     1561 ggaaacacta gaaactgtat tgctatctta cacgagggta accaaacaga aattttggaa
     1621 caaggaccaa caatttctca tgaagaagca gaaggctttc ttgaccacta tagcaatctt
     1681 attaaacaat cagaagttgt gacaatttca ggaagtttac cttcaggact tcccaacgat
     1741 tattatgaaa aacttatcca acttgcttcg gatgaaggag tagcagtcgt tttggattgt
     1801 tcaggtgcac ctcttgaaac agttctaaaa tcgtcggcta aacctactgc catcaaacct
     1861 aataatgagg aactttcaca gttgcttgga aaagaagtaa caaaagatat tgaagaactc
     1921 aaagacgttc ttaaagagtc actttttagt ggtatcgaat ggatagtggt ctcattaggt
     1981 cgaaacggtg cctttgcaaa acatggtgat gttttctata aggtagatat tcctgatatc
     2041 ccagttgtta atccagtcgg atcaggggac tcaacggtag ctggtattgc atcagcttta
     2101 aatagtaaaa aaagtgacgc tgacttatta aaacatgcga tgacattggg tatgttaaat
     2161 gctcaagaaa caatgacagg gcatgtgaat atgactaatt acgaaacact aaactcacaa
     2221 attggagtaa aagaggtata aaaatggtac ttacagaaca gaaacgcaaa tctttggaaa
     2281 aaatttcaga taaaaacggt gttatctcag ctttggcatt tgaccaacgt ggtgctttga
     2341 aacgtttgat ggcacagtat caagatacag aaccaactgt agctcaaatg gaagaactaa
     2401 aggttttggt tgctgatgaa cttacaaaat acgcttcttc aatgttgctt gaccctgaat
     2461 atggacttcc agcaacaaaa gcattggata aagaagcagg tcttcttctt gcctatgaaa
     2521 aaacaggtta tgatacatca agtacaaaac gtttgcctga ctgcttggat gtttggtctg
     2581 caaaacgtat caaagagcaa ggcgctgatg ccgttaagtt cttgctttac tatgatgtag
     2641 acagctcaga tgaacttaac caacaaaaac aagcttatat tgaacgtgtt ggttctgaat
     2701 gtgtcgctga agatattcct ttcttcttgg aaatccttgc ttatgatgaa gaaatctcag
     2761 atgcaggttc tgttgaatat gcaaaagtga aacctcgcaa agtcattgaa gcgatgaaag
     2821 ttttctctga tccacgcttt aacattgatg ttcttaaagt agaagtacca gttaacgtta
     2881 aatatgtcga aggttttgct gatggtgaag tagtttacag taaagctgaa gctgctgact
     2941 tctttaaagc acaagaagaa gcgactaacc ttccatacat ctacctaagt gcaggtgtat
     3001 ctgctaaatt gttccaagaa acattgcaat ttgctcacga ctcaggtgcc aagtttaatg
     3061 gtgtgctttg tggacgtgct acttgggcag gatctgtaga accttacatc aaagaaggtg
     3121 aaaaagcagc acgtgaatgg ttgcgtacta ctggattcga aaatattgat gaacttaaca
     3181 aagttcttgt taaaacagct agtccatgga ctgataaagt atagtaaaaa cataaaacgg
     3241 aggatattgt tatgaacaga gaagagatga ctctcttagg gtttgaaatt gttgcttatg
     3301 ctggagatgc tcgctctaaa cttttagaag cgcttaaagc ggctgaaaat ggtgatttcg
     3361 ctaaggcaga tagtcttgta gtagaagcag gaagctgtat tgcagaggct cacaattctc
     3421 agacagctat gttggctcga gaagcttctg gggaggaact tccatacagc gttaccatga
     3481 tgcatggtca ggaccactta atgactactg tcttattaaa agatgtgatt catcacctca
     3541 tcgaacttta taaaagagga gcaaaataat gcataaactc attgaactta ttgagaaagg
     3601 gaaaccgttc tttgagaaaa tctctcgaaa catctatctt cgtgctattc gtgatggatt
     3661 tatcgctggt atgccagtca tccttttctc atctatcttt atccttattg cctatgtccc
     3721 aaatgcttgg ggattccact ggtcaaaaga cattgagacc ttcttgatga ctccttatag
     3781 ctattcgatg ggtatcctag cattctttgt tggtggtact accgctaaag ctttgacgga
     3841 ctcaatgaac cgtgacctac ctgcgaccaa tcagattaac ttcttatcta cgatgctagc
     3901 gtctatggta gggttccttt tgatggcagc tgagcctgca aaagaaggag gcttcttgac
     3961 agccttcacg ggaacaaaag gtcttttgac agcatttatt gcagcctttg tgacagttaa
     4021 tgtttacaag gtctgtgtga aaaataatgt tactattcgt atgcctgaag aggttccacc
     4081 aaatatctct caagtattta aagacttgat tccattcact gtagcggttg ttctccttta
     4141 tggatttgaa ctcattgtta agggaactct tggtgtaacc gttgcagaat caatcggtac
     4201 ccttcttgct cctctcttct cagctgcaga tggttacctt ggaattacac ttatctttgg
     4261 tgcttatgcc ttcttctggt ttgttggtat ccacggtcct tcaattgtcg aaccagcaat
     4321 cgctgctatc acttatgcca atatcgatgc caacttgcat cttatccaag ctggacaaca
     4381 tgcagacaaa gttatcactt ctggtactca aatgtttatt gttaccatgg gtggaacagg
     4441 agctacattg attgttccat tcttgttcat gtggatttgt aaatcagaac gtaaccgtgc
     4501 catcggacgt gcctcagttg ttccaacatt ctttggggtt aatgagccaa tcttatttgg
     4561 tgcaccaatc gtattgaatc cgattttctt tgtaccgttc attttcgctc cgattgtcaa
     4621 cgtttggatc tttaaattct ttgttgacac attgaacatg aactcattct ctgccaacct
     4681 tccatgggtt actcctggtc cgttgggaat tgtactcggt actaacttcc aagttctatc
     4741 atttatctta gccggactct tggtagttgt tgatactatc atttattacc catttgttaa
     4801 ggtatacgat gaacaaattc ttgaagaaga acgttctggt aaaacaaacg atgctcttaa
     4861 agaaaaagta gctgcaaact tcaatactgc taaagcagat gccgttcttg gaaaagcagg
     4921 cgttgctaaa gaagatgtag cagcaaacaa taatatcaca aaagaaacta atgtccttgt
     4981 tctttgtgca ggtggaggta caagtggctt gcttgccaat gccttgaaca aagcagcagc
     5041 agaatacaat gttccagtca aagcggcagc gggtagttac ggtgctcacc gtgaaatgtt
     5101 gccagagttt gacttggtta tcttggctcc acaggtcgct tcaaacttcg atgatatgaa
     5161 ggctgaaacg gataaattgg gcattaaact tgcaaaaact gaaggagctc aatatatcaa
     5221 attgacacgt gatggacaag gtgctcttgc atttgttcaa caacagtttg attaataaat
     5281 ttgtaaaagt tatttgagac gagattattc tttcaattcc ttataatatt gttaaccata
     5341 tcattgcatt tcatttcctt ggtttccgga actcagaata tcgtctcaaa tgcttttttt
     5401 gaaaggattt atagttatga ctaaaacact tcctaaagat tttatttttg gtggtgctac
     5461 agctgcttat caagcagaag gtgcgactca cacggatggt aaaggcccag ttgcatggga
     5521 taagtatctt gaagacaatt attggtacac agcagagcct gcaagtgatt tttaccataa
     5581 atatcctgtt gatttagaat tagctgaaaa atatggcgtt aatggtattc gtatttcaat
     5641 tgcctggtct cgtattttcc caactggcta tggcgaagta aatgaaaaag gtgttgaatt
     5701 ctaccataat ctttttgccg agtgccacaa acgtcatgtt gagccattcg tgactttgca
     5761 ccactttgac acaccagaag ttcttcactc agatggagat ttcttaaacc gcgaaaatat
     5821 agaacacttt gtagattatg ccgctttctg ttttgaagag ttcccagaag taaactactg
     5881 gacaactttc aatgaaattg gcccaattgg agatggtcaa tacttggttg gtaaattccc
     5941 tccaggcatt caatatgatc ttgccaaagt cttccaatca caccacaata tgatggtttc
     6001 tcatgcacgt gctgtgaaat tgtataaaga taaaggttat aaaggcgaaa ttggtgtagt
     6061 acatgcgttg ccaaccaaat acccttatga tccagaaaat ccagcagatg ttcgtgccgc
     6121 tgagttagaa gatatcatcc ataacaaatt tatcttggat gcaacctacc ttggacacta
     6181 ttcagataaa acgatggaag gggtcaacca catcctagct gaaaatggtg gagaacttga
     6241 tcttcgtgat gaagacttcc aagcacttga agcagctaaa gatttgaacg atttccttgg
     6301 tatcaactac tacatgagtg attggatgca agcctttgat ggtgagactg aaatcattca
     6361 caatggtaag ggtgaaaaag gaagttctaa gtaccagatt aaaggtgttg gtcgccgaat
     6421 cgcaccagat tatgtgccta gaacggattg ggactggatt atttatcctg aaggcttgta
     6481 tgaccaaatc atgcgagtga aaaatgatta tccaaactac aagaagattt acatcactga
     6541 aaacggtctt ggatacaaag atgagtttgt agataatacc gtttatgatg atggtcgtat
     6601 tgattacgtg aagaaacact tggaagtttt atcagacgca attgcggatg gtgcaaatgt
     6661 taaaggttac ttcatctggt cacttatgga tgtcttctca tggtcaaacg gatacgaaaa
     6721 acgttatgga ctattctatg tagactttga cacgcaagaa cgctatccta agaaatcagc
     6781 tcattggtat aagaaagtag cagaaactca agtgatagag taatattact tgaaaatgta
     6841 aaaaattgtt atccgatact ttagaaaaag tacaacactt atttagacca ggctttgaaa
     6901 aggtatatat tgttagtttc gaagattgtc ctatgattcc tgagcttgaa gctaccccgt
     6961 tgctaaaatg tggtaaatgg tatgtttcaa ctggaaaaga gtggatttgc cattctgatt
     7021 tagagttatc agcatttgaa tgggaatttt tacaatcact cgatgtagaa atacgtgaga
     7081 ctattcactt cgaggtaaat tatttgcctt tccaataata aaaagaaagg aattagttgt
     7141 gactatcgaa ttaaaaaacg agtatcttac tgttcagttt aaaacacttg gtggtcaact
     7201 gacttcgatt aaagacaagg atgggataga gtatctctgg caagctgatc caaattattg
     7261 gaatggacag gcgcctattc tatttcctat ctgtggtagt ttacgaaatg attgggctat
     7321 ttatagacct caagaaagac ccttttttac aggtcttatt cgtagacatg gttttgttcg
     7381 aaaagaagaa tttattctgg aagaagtcaa tgataatagc gtgaccttta gtattaaacc
     7441 aaatgctgag atgcttgata attacctata tcagtttgaa ctgagagttg tttatacctt
     7501 gaatggtaaa tcaattagaa ccgaatttca ggtaacgaac ttggaaatgg aaaaaacaat
     7561 gccttacttt attggagccc atccagcatt caatagtcct ttagtagaag gtgaaaacta
     7621 tgaggattat tatattgaat ttagtgaggt tgagtcttgc tcgataccaa agagttttcc
     7681 tgagacagga ttattagatt tgcaagatcg gacacctttt ttggaaaatc aaaaaattct
     7741 cgatttggac tactcacttt tttcccatga tgccattaca cttgaccagc tcaagtctcg
     7801 tagcgtaacg cttcggtcaa ggaagtctgg taaaggtctt cgagtagatt ttgatgattt
     7861 tccgaacttg attctgtggt ctacaactaa taaaagtcca ttcattgctt tggaaccttg
     7921 gagtggtctc tccacttcac ttgaagaagg taatttctta gaagataaaa gacaggtaac
     7981 gaaaatttca cctcaagaaa cttctagaaa aatctatgat attactatcc ttaattaaaa
     8041 tttgttatta tttgagtctt caataagatt tgaatactct taccttgtga tgatagattg
     8101 gtcttctccg tgccagtctt acatcgcaag gtttttaagt tttttgtggg tctatgtcaa
     8161 ctgtagtggg tgacgaaaag ctaatatcta aagacagtct ggtagtaaaa aataagatta
     8221 tttactactt cttgatttaa aataatagtg gagccattaa tttgtggcaa atttaattta
     8281 atattttgcc tcaaattagc cacacttttt atagaactaa gaggaaaaat gaaattttct
     8341 tcctattaat atagttgttt gaaatctt
//
LOCUS       DACEJR010000101         8210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEJR010000101 DACEJR010000000
VERSION     DACEJR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.104"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            139..1332
                     /locus_tag="IW651_002624"
     CDS             139..1332
                     /locus_tag="IW651_002624"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0815302.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1462..2934
                     /locus_tag="IW651_002625"
     CDS             1462..2934
                     /locus_tag="IW651_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0815303.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3017..3805
                     /locus_tag="IW651_002626"
     CDS             3017..3805
                     /locus_tag="IW651_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0815304.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3872..4540)
                     /locus_tag="IW651_002627"
     CDS             complement(3872..4540)
                     /locus_tag="IW651_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0815305.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4655..6367
                     /locus_tag="IW651_002628"
     CDS             4655..6367
                     /locus_tag="IW651_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0815306.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6370..8142
                     /locus_tag="IW651_002629"
     CDS             6370..8142
                     /locus_tag="IW651_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0815307.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2514 a         1380 c         1807 g         2509 t
ORIGIN      
        1 tattttttaa gcaaaaaaat gaaaaaatac gagcagaaaa ctgttttatc tgctatactt
       61 ggttcgttgc aagttcccga aaggatccgc aggacggaga tgccttgtaa ccgaatttaa
      121 ctagggggaa tcattataat ggtagaaaga cacttattta catcagaatc tgtttctgaa
      181 ggacatccag ataaaattgc tgaccaaatc agtgatgcaa tcttggatgc aattttaaaa
      241 caagatccaa cagcacgagt agcgtgtgaa acgtctgtaa caactggtct tgttttagta
      301 tttggagaaa tatctacgac ggcatatgta gatattcaaa aagtcgtacg ggaaacaata
      361 aaagaaattg gttatacacg tgcaaaattc ggatttgatg gagatacagc agctgtattg
      421 gttgcgattg atgaacaatc tcctgatatt gctcaaggag ttgatgaagc acttgagatc
      481 cgggatgaag ataaaaaaga cgtattagat gagattggtg ctggtgacca aggtttaatg
      541 tttgggtttg ctgtagatga aacaccagaa ttgatgcctt taccaatcgc tttgagtcat
      601 cgtctggtac gacgcttggc agatttgcgt aaatcaaatg aattaacgta tttacgtcca
      661 gatgcaaaat ctcaagtaac ggttgaatat gatgatcaag gacaaccgga acgcgtagat
      721 acaatcgtta tttcaacaca gcatgatgat gcagtagata atgaaacaat tcgtcatgat
      781 gtcatcgaaa aagtagtgaa ggaagttatt ccagctgaat tattagatga tcaaacgaaa
      841 tattatatca atccgactgg ccgatttgtc attggtggtc ctcaagggga tgccggatta
      901 acaggaagaa aaatcattgt tgatacgtat ggcggttatg ctcgtcatgg tggcggtgct
      961 ttttctggta aagatgcgac aaaagttgac cgttctgcca gctatgctgc acgttatatt
     1021 gctaaaaaca ttgttgcagc agggcttgct cgcaaagcag aagtacagct agcttatgcg
     1081 attggtgttg ctcaacctgt ttcgatctca atcaatactt tcggtactgg aacggttccg
     1141 gaagaagaac tgattgctgc agtgagagaa aacttcgatc ttcggccagc aggaatcatc
     1201 gagatgcttg atttgcgtcg tccgatttac aaacaaacag ctgcttacgg tcattttggt
     1261 cgtacagatg tagatttgcc ttgggaacat acggacaaag tagatgcgtt gaaagcaagt
     1321 ttagcaaaat aagttatatt cagctggaac gatcgccaag tggtggttgt tccagctttt
     1381 tttatggcag ttaacctctt gaaaaagata gtaattattt atctattttt agatcctgtc
     1441 ataatgaaag ggaggaataa gatgaaaaaa gaaacaaatg ttactttggt cacagtgagt
     1501 attttcattg caacgttcat gacggctatt gaaggtacaa tcgtaacaac cgctatgcca
     1561 acgattgtcg gttcattaca tggaatggaa atcatgaact gggtattttc gatttatttg
     1621 ctgacaaacg ctatgcttac tccgatttac ggaaaacttg cggataaagt gggacggaaa
     1681 ccaatcttta tttttgggac attattgttt attatcggtt cgttattttg cggtttatct
     1741 caaagcatga ttgggttgat tgctgctcgt gcgattcaag ggatgggtgc tggggcaatg
     1801 atgccagtag ctttgacgat catcggcgat ctttattcta ttgaaaagcg agcaaaagtt
     1861 ttaggtttga atagttcagc ctgggggatt gccagtgttt ttggaccgct tgcgggagga
     1921 ttcatcgttg aaacgatcag ctggcactgg atctttttta tcaatgtgcc gatcggaatt
     1981 ctattaatta ttttgatttg gctatattta aatgaaccaa aaatcataca tgagtctaaa
     2041 ccaatggata tttttggtag ttttagtttg atggccatgc ttgtctcatt gctgttaggg
     2101 ttccaattat taggagacga aggaatgagt ccgcttgttt ctgctttgct gctaggtagt
     2161 atcgtaatgt taggtatttt tgtttttgcc gaaaaaagag ctaaagatcc tgtcatcatg
     2221 atgcatttat tcaaaaatcc actatttgtt gtggtcaact tggctgcagc attggctagt
     2281 ggttttttga tgggaatcga tgtttatatc cctatgtgga tgcaaggtgt attagggctg
     2341 aatgcagcaa ttggtgggct ggtattagca ccattatcac tagtctggat ggtcggttct
     2401 ttccttgcaa gcaagtggat gactcaaatg aaaactcatt gggtcttgag aattggacta
     2461 ctaatcgcag ctattggggg cgcttggttg tatcttatgc ctttcagaag taactattta
     2521 tggttttttg ctatatcaac ggtcatcggt ataggcttag gtgtgaccat gactacctcg
     2581 actgtcagtg cacaaaatag tgtatctcgt gaccagcttg gtgtagcaac ttcttttaat
     2641 acgttagttc gaacaattgg tcaaacagtc atggtttcta tttttggttt attgataaat
     2701 ggggtaacta aacgagaact tgcagcagca aatttaacaa atgatgcaga tatcatgaat
     2761 aaactggtga atccacaaac ggctaaatat attgatgcgg atattttgtt accattaaga
     2821 aaaattcttt atgatgggtt acattccgtt tatctagttg gcttgttact cgtgatcatt
     2881 gctttggtct taacgctttt cgttcatgaa aaaagtggta aaataaaaca gtagtttcgg
     2941 ttaagatatg tacgtagccg aatcattcaa atttaaagtt attcaaaaat aacttcttac
     3001 taaggaaggc gaaaatatgg catttattca agcaaatatt tattcgaatg tgttagaaat
     3061 ggaagtcaat ctaaatgtga tactaccgca aacaacaaaa aaggtgattg gcaccagttc
     3121 agagcaagag acaacggata tacctgtttt atatttactt catggaatgg gtggcaatca
     3181 tagtgtatgg gaaagacgta cgtcgattga acgttatgta gcaaactacg gattagctgt
     3241 catcatgcca tctactgatc ttggctggta cacagataca gtttatgaca tgaagtattg
     3301 gacattcatt gcagaagagc tacctgagat ttgtcatgaa ttattcccgc aattaacaag
     3361 aaaaagagaa aaaacatatg ccgctggatt atcgatgggc ggatatggcg cgttaaaact
     3421 aggccttgca aaatcagaaa atttcgcagc agtaatctct ctttcaggcg ctgtttctgt
     3481 aggaaatcga atggatgatc ttttgatggt tcgtaaagca tctttttggg aagggatctt
     3541 tggaccattg gatcaaatcg aaggttcaaa aaatgatccg caatacttat tgaaagaact
     3601 agtaaaaagc ggaaaaccag ttccaagatt ttatatggct tgtggggaat ctgattttct
     3661 ttatcaggca aatcaggaaa tggtatctgc tatgcaggaa caaggcttgg cggtcacctt
     3721 tgaacaagga cctggcgatc ataactggat cttctgggac caatggattc aacgtgcatt
     3781 ggcgtggcta atggaagaaa aataaaaaaa gagtcttaaa aagatagctc ctatagagat
     3841 cacaaacata gtgatctcta taggagcttt tttactgctc gttttctctt cttttatttt
     3901 gttggcataa gccttcaata taggtttgtg cactcgttag aaagagtgct ttttcattca
     3961 attctgcatg ggactgcata ctttctttaa atccctctaa ttgattttca actacataat
     4021 tacgcataaa tagaactgtt tctttcaaaa attcatttcc gtccataatt ttttgtcgaa
     4081 gctgctgttc atgctgctga tccattaact gtccaatcaa taaattgtgg agagaggcaa
     4141 tggcctgatg actttgggtg actgaaaatc cttcacgaac aagtaattgg atcatttgct
     4201 ccaaatgttc taatcgactc ggaaaagcgg gcacagtatt gatttctaat tctgccccac
     4261 aagggaaacg tgtataaagg tcatagtaat tttccatata ctgcagcaac tgttcattcc
     4321 aaggcaaagt tggatctgga agcgctaatt cttcttccat tgtttcagcc attttttgca
     4381 agagctgttg tttgtttttg aaataccaat ataaagcagg tgcttgaaca tgaattctgt
     4441 ttgcgatgtt tcgcatggaa agctgcgaaa gagcttgtgt ttcagccagg atatcaaagg
     4501 cggcctgaat gatgattgct tgagaaagct ttatttccat tttatcaacc tcgtgtattg
     4561 cattatcttt ttaaacatgg taaattgtta gtcggtctaa ttttacagtg ttaaattagt
     4621 cctgtcaatt taaacaaagg ggtgtaatca acgaatgaaa ttaatcttac gacatgcgaa
     4681 aaaatataaa gtagctgttt ttatttcctt gctgtccgta gcagtcatgg ttgcagcagc
     4741 attatggcaa cctaagttac tacagcaagt attggaagct attattacag aagataatga
     4801 tgaaatgaaa acaattggta tttacctgat cagtctggct ttgctaggat tagccgcagg
     4861 tgtaacgaac acgattttct ctgcaaaagt agctcaaggc gtaagtgcag atatccgaga
     4921 agaagcattt cgaaagatcc agacattttc atttggaaat atcgaacagt tctctgctgg
     4981 aaatcttgtt gttcgtctaa caaatgatat cacgcaaatc caaaatttag tgatgatttc
     5041 tttgcaatca ctttttcgaa ttccattctt atttattggt gccttcattt tagctatgat
     5101 cacgatgccg caattatggt ggatcatcgt agcgcttatc gttacggtat ttctgattac
     5161 ggcactttca tttacaagga tgggcaagca cttcatgatc attcaaaaac tgattgatcg
     5221 tgtgaatagc attgcaaaag aaaacttact agggattcgt gtagtgaaat cttttgtaca
     5281 agaagaaaac caattgaagt cattcagcaa agtaagtgag gacttaacaa aacacaacat
     5341 catagtcggt acattatttt ctgtgatgat cccgtccttc atgctggctg ccaatttggc
     5401 tgtggttggg gcgatctttt ttgtgagcga tcttgttaaa gatgatccta cgttaattgg
     5461 tggaattgct tcattcatga actacttgat gcagattatg atggcaatca ttattggcgg
     5521 aatgatgatg atgatgactt caagagcagc cgtatcgctg aagcggattg cggaaatttt
     5581 agatgctgaa ccagatctta cttatttaga cgttccagaa caagagttga cgggttctgt
     5641 cacttttgac catgtttctt ttcgttatcc tggtgaggat acagatacgc ttaaagatat
     5701 cagcttttct atcaaacctg gagaaatggt tggtatcgtc ggggcgactg gtgccgggaa
     5761 gtcaacacta gcacaattga tccctcgtct atttgatccg acagaaggca aagttgaagt
     5821 aggtggtgta gatcttcgtc aagtaaatga aaagagttta agacaaacgg tttcctttgt
     5881 tttgcaaaaa gcaattttat tctcaggaac gattgctcaa aacttgcgcc aagggaaaaa
     5941 gaatgcaaca gaaaaagaga tggaacatgc ttcttctatt gctcaagcaa aagaattcat
     6001 cgaaaagtta tctgatcgtt acgaagcacc aatagaagaa cgtagcagta acttttctgg
     6061 tggtcaaaag caacgtttat ccattactcg tggcgttatc ggtaatccaa agatcttgat
     6121 cctagatgac agtacgagtg cactggatgc tcgctctgaa aagctggtcc gagaagcact
     6181 cgatcgagat ttgaagggaa caacgacgat tgtcatcgca caaaaaattg cttctgttgt
     6241 caaagcagac cgcatcttag tattggatga aggcagatta gtcggcgaag gaacccatga
     6301 ggaattggta gctggaaacc gtgtctatca ggaaatcttt gaaactcaaa aaggaacgga
     6361 ggagtaagta tgactgatct aataaaagca agtaaatttt tctatcacta tttgaagcgg
     6421 tacaaactct cctttttctt tatctttatc acagtgatca ttgctaccta tctacaagta
     6481 aaggcacctc aatacatcgg ggaagctttt caagaattag ctaattacgt aggaggcttg
     6541 atgcaaggcg tagacgataa aagcaaattt gtttctgtaa tatggaaact cttgctgttt
     6601 tatgtactag ctagtgcagc caactttatc tatagtattt tgttcacaca agtagtaggg
     6661 aagtcaacga accgaatgcg tatcggtcta ttcaacaaat tggaaaaatt gacgatccgt
     6721 ttctttgatt ctcatcaaga cggtgaaatt ttaagtcgtt ttaccagtga cttagataat
     6781 attcaaaata gtttgaacca agctttgctt caagtcatca cgaatgctgt tttacttgtc
     6841 gggatcttga tcatgatgtt ccgccaaaat gtagagttag cttgggctac aattgcttcc
     6901 actccagtag ccgttttgat agctgtcttg attatccgca aagcacggaa atatgtggat
     6961 cttcagcaag atgaagtggg gaaactaaat ggttatatgg atgaaaaaat cagcggacaa
     7021 cgtgtcatca tcacaaatgg cttacaagaa gaaacgattg aaggattctt agagcataat
     7081 caaagtgttc gcgaagcaac cttcaaagga caagcttatt ctggtctact atttccgatg
     7141 atgcaaggaa tgtcactggt caatacagcg atagttatct ttttcggcgg ctggttagca
     7201 ttaaatggtg atttggaacg ttcggtggca ttaggcttag ttgtgacatt cgtccagtat
     7261 tcacaacaat attaccaacc actgatgcag atttcatctg gatacagcat gatccaatta
     7321 gcgattacag gggcaagacg attgaatgaa atatttgatg aaaaagatga agtcaaacca
     7381 gaaaacggta aagcatttga tggtgttgaa aaaggtcttg cgttgcagca cgtagatttt
     7441 ggttattctc cagaaaaact agtcttgaaa gatgtctcga tcaatgtgga caaaggagaa
     7501 atggttgctc ttgttggtcc aacaggatcg gggaaaacga cgatcatgaa tctgctaaac
     7561 cgtttttacg atgtcaatga tggttcagta gtcatcgatg gaatagatat ccgagattat
     7621 gatcttgatg cattacgtag acatgtagga atcgttttgc aagaatctgt attgttctcc
     7681 ggaacgatca aagacaatat cacattcggc aaaccggacg cttcagatga agaagtcatc
     7741 aatgcagcaa aacaagcgaa tattcatgac ttcatcatga cgttagaaaa aggttatgat
     7801 acagagatat ccgaagaaaa taacttattc agtacaggtc aaaagcagtt gatcagtatt
     7861 gctcgaacga tcatcacaaa tccatcttta ttgattttgg atgaagcaac aagtaatgta
     7921 gacacggtaa cagaagcaag gatccaaaaa gcaatggatg aagcaatcaa aggaagaacc
     7981 agctttgtga ttgctcaccg gctgaaaaca atcttgaatg cagatagaat cgttgtcttg
     8041 cgtgatgggg aagtcattga agaaggcagt catcatgagt tattagcaga aaatggattt
     8101 tatgcggaac tttatcataa tcaatttgta tttgaaagtt aaattaaaag aacctcgtta
     8161 taacggggtt ctttttatat attttatgtt gtttgttttt ataaggggca
//
LOCUS       DACEJR010000102         7684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEJR010000102 DACEJR010000000
VERSION     DACEJR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.106"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            108..485
                     /locus_tag="IW651_002630"
     CDS             108..485
                     /locus_tag="IW651_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0815308.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            473..811
                     /locus_tag="IW651_002631"
     CDS             473..811
                     /locus_tag="IW651_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0815309.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            945..1388
                     /locus_tag="IW651_002632"
     CDS             945..1388
                     /locus_tag="IW651_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0815310.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1650..2465
                     /locus_tag="IW651_002633"
     CDS             1650..2465
                     /locus_tag="IW651_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0815311.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2475..3137
                     /locus_tag="IW651_002634"
     CDS             2475..3137
                     /locus_tag="IW651_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0815312.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3342..5006
                     /gene="alsS"
                     /locus_tag="IW651_002635"
     CDS             3342..5006
                     /gene="alsS"
                     /locus_tag="IW651_002635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0815313.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5019..5729
                     /gene="budA"
                     /locus_tag="IW651_002636"
     CDS             5019..5729
                     /gene="budA"
                     /locus_tag="IW651_002636"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0815314.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5859..6356
                     /locus_tag="IW651_002637"
     CDS             5859..6356
                     /locus_tag="IW651_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0815315.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            6541..6801
                     /locus_tag="IW651_002638"
     CDS             6541..6801
                     /locus_tag="IW651_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815316.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(7163..7402)
                     /locus_tag="IW651_002639"
     CDS             complement(7163..7402)
                     /locus_tag="IW651_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815317.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         2551 a         1176 c         1597 g         2360 t
ORIGIN      
        1 agcttagttt taatagaaat gagtattgac ttggagtaca cttaaagttg tagaataaat
       61 aaaatacata agtaaagtgg caagaaatta agagaaagag tgatcagatg aatatcaaac
      121 aagtcagtga agaaaagggc atttcggccg atacattgcg ctattatgaa cgaatcgggt
      181 tgattccacc agttaatcgg acaaaaggcg gaatccgtga ttatacagaa gaagacctaa
      241 aatgggtcga ctttacgatc tgtatgcgaa atgccggatt atcgatcgag tctttgattg
      301 aatatattcg cctatctagc gaaggagaag caactgtatt tgagcggcgt aagcttttga
      361 ttgaagaaag tgaacttttg acgaaaaaaa ttgctgaaat gcaagaatgt ttggaacgtt
      421 tacaaggaaa aatcaagaaa tatgatcgtg tgttatctag aaataaaatg gaatgtatca
      481 agtaaaaaaa tcaaggagca tcgctatgga gttacgctta ctaaaatatt tttggacaat
      541 tgcagaagaa ggaacaatct ctcaagccgc agaagttcta catcttacac aaccaacctt
      601 aagccgacaa ctgaaggagt tagaagagga actaggaaca gaactgtttg tacgcgaaaa
      661 ccgcaaaatg atcttgacag aagcaggtta ttttctaaaa aagccgagca gaagaaattt
      721 tagatttaac gaatcaaacc actcaagaat tcgagaacag aaaaaaacaa ttattcagcg
      781 gccgatttac gatcggctgt gtggaagctg ataattcgaa tacattggca ctattactag
      841 aaaagttgat ccgtgattat cccagcgttc aatttcatat ccacagcgga acaagtgatg
      901 atatcacgga gtgactagaa aaaagcctgt tagatttagc gattttgcta gatccagtag
      961 atacagagaa atacgaaacg attttgttac caagaaagga aaaatgggga ttgctcattt
     1021 tagaaaattc tgttttagca aaaaaagacg tgataacaaa agaagacttt gcgaatgtac
     1081 ctcttttgat ctcaaagcgt ccagaaatac agcgaatgtt tgctgactgg ttaggcataa
     1141 aaaaggagca gttgctgatc acaggaacgt acaatttgat tttcaatgta tttgcgctaa
     1201 ttgagaatca ggtaggtgct gctttagtca tagaaggtgc tgtaagtcat ctttcaaagc
     1261 gagtgacatt cgttccgctt gacccaatga ttgaaacgaa ctgcatgcta gtctggaaaa
     1321 agaatcggat tttatcacct gtagtcaatg aattgattca ccgtttcaaa gaagcttttg
     1381 aacagtaata agtatctcta atcgaagaaa tttcaataca aaagcgagac gtatgacagt
     1441 cttgcttttg tattttttca tttttaaatc aaaaaaaata aagagattta cataacttgt
     1501 taaataagcg cttacaaaca taagaagaac ggaaatgaat cagctataat aaccgttgtt
     1561 ctggattgta atcattacaa agaaatgaag attataactt tggaggtgtt cgaaatgaaa
     1621 gaaaaaaagc aaaaattcgt tcaaaaaaca tgccctttat tcaatggttg ggtatgattc
     1681 tgctgattgt attagcttta ttgacgatca ttcctttttt atggatggtt tctgcttcat
     1741 tcaaagcaaa taatgaagtg ttcacgattc cgattcaatg gatccctaaa acttggcatt
     1801 tagagaatta tctggttatt tggtctcgta ttcctttagt gacatttttt aagaatacag
     1861 cttttttaag cgttgtgatc acgttgattc agttgtttac ttctagtttt gctgcttacg
     1921 ggttttctaa actaaatttt aaagggcgcg atattttatt tttagcatat atcggcacga
     1981 ttgctgtccc ttggcagtct tatatgatcc cgcagttcat tatgatgaga aaagtgggtt
     2041 taacatatac gttgtggtcg ttggtgctat tacaagcgtt caatgctttt ggtgtgttct
     2101 tgttgaagca atactacagt agtatcccag attctttatg tgagagtgcg cggatcgatg
     2161 gcttatcaga gtggggaatc tatcgcaaga tcattttacc tcttacaaaa ccggcgattg
     2221 ccagtttgac gatcatcaca ttcgttaata catggaacga ttatatgggg ccattcattt
     2281 atttgtcttc tacggaaaat aaaacaatcc agttagggtt gaaaatgttt attgggctat
     2341 ttgatgcaga gtacgcatta atcatggctg cctcagttgt ttctgttctt cctgttgctt
     2401 ttatattttt agcgatgcaa aaatattttg tagaagggat cgcttcatca ggaatgaagg
     2461 aataggaggt gattatgaaa gagcagcgtt ttgacaaaaa tgtttatatg gaattctttt
     2521 attggttgta tcaatcattg gcaagtagcc tttgtttttg gtgtgtcaat atcttgtata
     2581 tagcagctgc gttccttttg tcgatagatg gaaggaatgt cttttccttt attttttctt
     2641 tactttttat tggtccaggt tgcatcgtga tgataggtat gacagaagaa ttagcaaaag
     2701 aaaaaagttt ttcaccagta cgtttgttca taaagacgtt tcgccaattt tggctaaagg
     2761 gattttttta ttggctattt gcttggatag ttagtgtgat aatgattttt gattgtttct
     2821 tttttatacg tttttcttat ggaaaatggt tgattccgtt gtttgtttta cttgcttgtt
     2881 tatcagtttc ctttagcatc aactactggt attttcaggt gagaaatcca gcgtcgaaac
     2941 caaatcaagt tctacggata gcgttctact atacgttgaa aaaatggtat gtgagtttac
     3001 tagatttttt actattgaca agtttgtttc tcttcttttt cgtaaagccc caatggtgta
     3061 ttctactagg tccttctatc gtatttggtt tgatttactt caacaatcgt aaattgatga
     3121 gaactatgga tctgtagact ttataaattg aaactttcaa ttaagaatga cagtttttat
     3181 gtaaaaactg tcattctttt tcattttttg aaacatatgg ctaaacaaat gaaataaaac
     3241 gtgctatact tttgaatgaa acaggtttag aaactgtatc aattatttgt gattatttct
     3301 aactaataat taaaaataaa gcataaaaag gacgatgttc aatggacgaa aaaatgaaac
     3361 aaggttcgaa atctgtagta gaaagtttga ttaatcatca tgttgattat gtattcggta
     3421 taccaggtgc aaaaatcgat ggtgtattta atgaattaga ggatcaaggt cctgaattaa
     3481 tcgtgactcg tcacgaacaa aatgctgcat ttatggcaca agcaatcgga cgtattacag
     3541 gagagccagg agtagttatc gcaacaagtg gtcctggtgc tagtaactta gctacaggtt
     3601 tggtaacagc gacagcagaa ggtgacccag ttttagcaat tgccggacaa gtaaaacgaa
     3661 gcgatctatt gaagttgacc catcaaagca tggataatgc agcactgttc cagccaatca
     3721 caaaatacag tgcagaaatc caagatccag aaacaatttc agaagtgatt gccaatgctt
     3781 atcgtatggc taagagttca aaaaaaggcg cgagctttat cagtatcccg caagatgtag
     3841 tggatgcacc tgtccaaggg aatgtcatca agccattgag cgatcctaaa ttaggttctg
     3901 cttctgcaga tgatatccgt tacctagctg aacgcattag agaagcaaaa ctaccggtac
     3961 tattagtagg tatgcgagga tcaagtgaaa aagaaacgtt agccatcagg caattagttg
     4021 gaaagacagc attgcctgtt gtagagacat tccaagcagc tggagtgatc tcacgtgaat
     4081 tagaagcgca tttctttggt cgtgtgggtt tatttagaaa ccaaccagga gatatgcttt
     4141 tgaaacgaag tgatctagtt attgctattg gctatgatcc aatcgaatat gaagcaagaa
     4201 actggaatgc ggaaaaagat gcgcggatca ttgtgatcga tgaagcgcca gctgaaatcg
     4261 atccttttat gcagcctgaa agagaattga tcggtgatat ctcagccact cttgatttat
     4321 tgacaggttc attagagcct caacaagtgt cagaagatgc aaaagaatat ttagcatctc
     4381 ttcaagcaga attaacggag cgggacattg ttcaatcaaa aggagaagca ggtatcttgc
     4441 atcctttaga agttattaac acgcttcagt caaaagtaac agatgatatg actgttaccg
     4501 tagatgtagg tagtcattat atttggatgg cacgccactt tagaagttat gaacctcgtc
     4561 atttgttatt cagtaatggt atgcaaacgc ttggtgttgc attgccatgg gcgatttctg
     4621 ctgcattggt gcgtccaaat acacaaatcg tatcagtttc cggcgatggg ggatttttat
     4681 tctctgccca ggatttagaa acggcggtac gtaaaaaatt gaatatcgtt catttgattt
     4741 ggaatgatgg acactacaat atggtagagt tccaagaaaa aatgaaatat cagcgggcgt
     4801 caggtgtaga ctttggtcca gttgattttg tgaaatatgc agaagctttt ggcgctaaag
     4861 gaatccgagc tacaagtgta gaggaattag aaaaagcttt agaagaagga tttgcgacag
     4921 aaggacccgt aattattgat attccgattg attaccgtga taatgagaaa ttgggagaaa
     4981 caatcttacc agatcaattc tattaaggaa gtgtcgacat gactgaaaaa atcttatacc
     5041 agcacgggac attaggagct ttgatggctg gcttgatgga tggaacagaa acgattgctc
     5101 atatattaga aaaaggaact ctgggattgg gaacattgca tgggcttgat ggtgaagtca
     5161 tctttttaga tggcgtagct tatcaaggaa gatcagatgg ctcagttgcc caattagatg
     5221 gaagtgaact taccccctat gcagcaatta cagattttac gccagatact agtttttctg
     5281 tctcaaaaac tgcagatgga gaacaattga aaaaagacat attagtaaga gaagccgggg
     5341 aaaacctatt tttggcagtg aagataactg gattattcaa aaatatgcac atccgtatca
     5401 tgccgaagca agaaaaacct tacagaagac tagcaaaaat ttcagagtct caaccagaat
     5461 tccaacaaag caatgtccaa ggaacattag tcgggttctt tacgcctgaa ttattccaag
     5521 gagttgctgc agcgggattt cacttgcatt ttatcgatga tacacataca tttggcggac
     5581 atgtaatgga ttttgaagtg gctgaaggaa aagttgagat tagccgcatc tcgtcccttg
     5641 tacagcattt tccagttgat gatccgactt ttgttcacga aacgattgat tatgctgatt
     5701 tagcagcaga aatccaacaa gcagaataga aaaagacagc gagatgcgtt caaggtgatt
     5761 aacctgacgt atctcgtttt tttgaggagg aaacctagcg tttacaaaaa aactgagcta
     5821 taatcttctc catagatcaa aaaggagcgg tttttatcat gtggacagaa aaacagaaaa
     5881 aagatgcata cgaaatttta ttattacagc aacaaggatt attagaagca gaagataact
     5941 ggttagcaaa tttagcaaat tcatctgcat tgttgaatga gactttgcct gagactgtct
     6001 ttgcaggata ttatttattc gatggtaatg aattgatttt aggaccgttc caagggcgag
     6061 tttcttgcac acgtatcaag atgggaaaag gagtttgtgg agaatcagct gaaaaacaag
     6121 aaacattgat cgttgatgat gtcaaaaccc atgcaaatta catttcctgc gattcagccg
     6181 cacaatcaga aatcgtagtg ccaatggtaa aagaaggtaa tttaatcggt gttttagata
     6241 tcgattgcgg agtaacgaaa gggtatgatg aaatcgatca agcatttcta gaaaaatatg
     6301 tagatctttt attagcaaat tttaatataa atgctttttc atttattaat aactaaaatc
     6361 gtctattgaa acaaaaaaga agagaaatca agaataaaaa gagccggaaa gacagtgggc
     6421 atttctgttt ttcggttttt ttattttttt ataaaatcac aagtgttatt gattttcata
     6481 atcatatttt aatggtatta tcattttaaa taatggaaaa acattaagga tggatgaaat
     6541 atgaataacg aaactatcat tttaggaaaa aaatatattt gccaacctat tggcttgaaa
     6601 caaaaagtgg ttggagaagt aatcagtaaa ttagaaaatt gtgttgtgat ctgcgtagac
     6661 gaatatcatg acgtcgatcg caatgaaata ttagaaaaat gcggaaaagt agtggcaaaa
     6721 tatactcacg tttatgagtt ggaaacaaca gaatatctat ataactatag caacaaatat
     6781 gaacatactt tagtttcata aagatttaaa taaagtgcct tcaaaagcga gaagaccaag
     6841 cttttgaagg cactttattt agttggcagt gattgttcat gatttttgat ttattcaaaa
     6901 ggttcgccta gagaatctgt ctctgtaacg aggcctggtg gagaaactcg ataaaaacct
     6961 atactgccag aactgtccta ttttcggata gacaaactag tagttttaaa aagatcatcc
     7021 gatccaatca tttcaaaaaa tgcaaaactg atatcaggat catttagctc aggcttgtta
     7081 tcgtaaagca tttgtaaagg ttgcatttag ttaggagtaa acgcgaaact atcatcatta
     7141 tttgctgaag aagatgaaga agctaattca gagtttgttt gaaataaagg agacgtgctg
     7201 acagtttctt taccctgttt tttgcagagg tagaaacttt tatatcggaa gtttctaccg
     7261 gagaattttt ctcatttgta gaaattgttg aatcgatact attcgaaatt tgcgtatctg
     7321 aaggtatgtt ctttgatgca gaagtcccac aagcagtaag taacaataaa tttgataaac
     7381 tgataaacag aattcttttc attatgtaca cactccttat agtatcgttt agcatacacc
     7441 tatgctcagt attaggcaaa atcaatgcaa aactatcaag tcttttatag tatctattag
     7501 tcttagtaat acatatatgg agagggtgat atcccagttc aaaccagata cattggttac
     7561 catctgtatt aacgattatg cctctaaacg ctttttttat gaaaaaattt ataagtgaaa
     7621 acgtaggtat gatattatct agaggtaatt tttttgggta tttaaatagt tgctaggagg
     7681 atag
//
LOCUS       DACEJR010000103         7598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEJR010000103 DACEJR010000000
VERSION     DACEJR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.107"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..427)
                     /locus_tag="IW651_002640"
     CDS             complement(137..427)
                     /locus_tag="IW651_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815318.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IW651_002641"
     CDS             complement(545..805)
                     /locus_tag="IW651_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815319.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IW651_002642"
     CDS             complement(942..1355)
                     /locus_tag="IW651_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0815320.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IW651_002643"
     CDS             complement(1811..2296)
                     /locus_tag="IW651_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815321.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2680)
                     /locus_tag="IW651_002644"
     CDS             complement(2435..2680)
                     /locus_tag="IW651_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0815322.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IW651_002645"
     CDS             2873..3673
                     /locus_tag="IW651_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0815323.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IW651_002646"
     CDS             3692..5560
                     /locus_tag="IW651_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0815324.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IW651_002647"
     CDS             5553..6044
                     /locus_tag="IW651_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0815325.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GIRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IW651_002648"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IW651_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0815326.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IW651_002649"
     CDS             6604..>7598
                     /locus_tag="IW651_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0815327.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1442 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga agctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtata cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg ttcccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACEJR010000104         7561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEJR010000104 DACEJR010000000
VERSION     DACEJR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.108"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..1390
                     /locus_tag="IW651_002650"
     CDS             68..1390
                     /locus_tag="IW651_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0815328.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            1545..2321
                     /locus_tag="IW651_002651"
     CDS             1545..2321
                     /locus_tag="IW651_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0815329.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            2314..2883
                     /gene="rnmV"
                     /locus_tag="IW651_002652"
     CDS             2314..2883
                     /gene="rnmV"
                     /locus_tag="IW651_002652"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0815330.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            2876..3760
                     /gene="rsmA"
                     /locus_tag="IW651_002653"
     CDS             2876..3760
                     /gene="rsmA"
                     /locus_tag="IW651_002653"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0815331.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4211..4840
                     /locus_tag="IW651_002654"
     CDS             4211..4840
                     /locus_tag="IW651_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815332.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(4964..6073)
                     /gene="ugpC"
                     /locus_tag="IW651_002655"
     CDS             complement(4964..6073)
                     /gene="ugpC"
                     /locus_tag="IW651_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0815333.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            6282..6704
                     /gene="mgsA"
                     /locus_tag="IW651_002656"
     CDS             6282..6704
                     /gene="mgsA"
                     /locus_tag="IW651_002656"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0815334.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7027..>7561
                     /locus_tag="IW651_002657"
     CDS             7027..>7561
                     /locus_tag="IW651_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0815335.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2335 a         1344 c         1590 g         2292 t
ORIGIN      
        1 ggtaaattaa aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag
       61 gatgggaatg aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt
      121 tatgggaacc gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac
      181 tcagtctaca agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc
      241 tggattgaca gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata
      301 tattgaagct gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat
      361 ttactttcct gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt
      421 attacaatta tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac
      481 agggttgaat ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt
      541 taaattaata ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac
      601 tgtccagtta tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag
      661 tgcgttgtta gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg
      721 ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt
      781 aacagtcgtt tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct
      841 tgccggtaat ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg
      901 aaagctggtc actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact
      961 aactgggatc cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca
     1021 gctgacaaag ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc
     1081 agtagcttgt attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt
     1141 tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc
     1201 gccggaattg ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat
     1261 tctcggtgct attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat
     1321 cggtataggt gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc
     1381 agaagagtaa aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt
     1441 cgtgcttttt ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa
     1501 gtagtgctta tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc
     1561 atacccattt aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac
     1621 aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt
     1681 ctttgcaatt aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag
     1741 aggctggcag ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa
     1801 aggttgtagc attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag
     1861 tccaagataa agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca
     1921 gtattcatac tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc
     1981 gagatatcgg tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc
     2041 ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg
     2101 tccaagaggc tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt
     2161 atctggcacc agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag
     2221 tagaaaagat tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga
     2281 caaatgcgca ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt
     2341 atagtcgttg aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg
     2401 atagaaacaa tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa
     2461 gagactcgtg gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa
     2521 atcattatgg aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa
     2581 ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca
     2641 ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa
     2701 gaccttttag actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta
     2761 ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg
     2821 tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag
     2881 tgaatatcgt gaaattgcga caccctctag aacaaaagag attttattga aacatggttt
     2941 ttcatttaaa aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat
     3001 cgtagaaact gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg
     3061 tgcattgacg gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga
     3121 tcgtttgatc cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca
     3181 tcaagatatt ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct
     3241 tccgttaaaa gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt
     3301 tcttgaatca gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga
     3361 tcgtatctct gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata
     3421 ttatatggaa gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa
     3481 tgtagattca gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga
     3541 cgaaaaagca tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg
     3601 gaataacttg cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag
     3661 cttggaaaca gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt
     3721 cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa
     3781 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     3841 tgtttttcgt ttgtttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     3901 agaggttgtg ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt
     3961 ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc
     4021 cgaacaagga gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa
     4081 aattcttata cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt
     4141 gacagttatt agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga
     4201 gagagaaaaa atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg
     4261 gatgggggtc attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac
     4321 tttggcgtct catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc
     4381 tgttgcgcta ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg
     4441 agcattgtca catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag
     4501 tatcgtttta aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt
     4561 tgtgatcggt gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat
     4621 tggtaatatg cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat
     4681 ggggattggt gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg
     4741 aacactcagc aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa
     4801 agagtcgatt caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa
     4861 aactctcatc tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag
     4921 agctgcacca acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga
     4981 cttgttctgt ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta
     5041 tttcgcctgg tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag
     5101 tttttgtgta aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca
     5161 ctacggcttc aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa
     5221 taccgaagat aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt
     5281 caggaatacg aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat
     5341 taaagaagtt catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat
     5401 cataaacttc tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc
     5461 ggtcagccat agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa
     5521 gacgctggtg aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat
     5581 ttgataatgg ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg
     5641 ccacacgttg gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca
     5701 gacctaagat ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac
     5761 gtaatttcaa gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt
     5821 tttggaaaac catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc
     5881 cgatatttag ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg
     5941 actttccaca accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat
     6001 taaaatcagt tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag
     6061 ccatttctac cataatacgt tccacacttt cttttttagt tttattaact atttaaagta
     6121 taaatgaaag cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg
     6181 tcaatgtgct agttttaagc tttaataaat atccataaga aataaaaact gatacactgg
     6241 agtaaataag tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg
     6301 ctcatgaccg aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg
     6361 aaaaacatga actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct
     6421 tgccgatcca tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga
     6481 tttcggagga tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc
     6541 atgaaccaga tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta
     6601 cgaatatcgg aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc
     6661 gaagaatcgt ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga
     6721 aaaataacgt ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg
     6781 cgatataata cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag
     6841 taaatgtgaa aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag
     6901 aaaataaagc gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg
     6961 ttcgcggttt tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg
     7021 ctaaaaatga agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt
     7081 gcattgatgg tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt
     7141 gtcacattta ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata
     7201 ggcgcagttt tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt
     7261 ggatttacat tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa
     7321 gagatcactt ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta
     7381 tttttgacac cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt
     7441 tgggcgcccc gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat
     7501 tacgtaggac gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa
     7561 t
//
LOCUS       DACEJR010000105         7401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEJR010000105 DACEJR010000000
VERSION     DACEJR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.110"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            69..152
                     /locus_tag="IW651_002658"
     CDS             69..152
                     /locus_tag="IW651_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0815336.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="IW651_002659"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="IW651_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0815337.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1184..1444
                     /locus_tag="IW651_002660"
                     /pseudo
     CDS             1184..1444
                     /locus_tag="IW651_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1607..2272
                     /locus_tag="IW651_002661"
                     /pseudo
     CDS             1607..2272
                     /locus_tag="IW651_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2308..2553
                     /locus_tag="IW651_002662"
     CDS             2308..2553
                     /locus_tag="IW651_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0815338.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2656..3525
                     /locus_tag="IW651_002663"
     CDS             2656..3525
                     /locus_tag="IW651_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0815339.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3506..4240
                     /locus_tag="IW651_002664"
     CDS             3506..4240
                     /locus_tag="IW651_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0815340.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4273..5185
                     /gene="ant(6)"
                     /locus_tag="IW651_002665"
                     /pseudo
     CDS             4273..5185
                     /gene="ant(6)"
                     /locus_tag="IW651_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5182..5724
                     /gene="sat4"
                     /locus_tag="IW651_002666"
     CDS             5182..5724
                     /gene="sat4"
                     /locus_tag="IW651_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0815341.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5817..6611
                     /locus_tag="IW651_002667"
                     /note="aph(3')-IIIa"
     CDS             5817..6611
                     /locus_tag="IW651_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0815342.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6719..6907)
                     /locus_tag="IW651_002668"
                     /pseudo
     CDS             complement(6719..6907)
                     /locus_tag="IW651_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6887..>7401
                     /locus_tag="IW651_002669"
     CDS             6887..>7401
                     /locus_tag="IW651_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0815343.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1555 g         2134 t
ORIGIN      
        1 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1081 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1141 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1201 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1261 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1321 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1381 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1441 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1501 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1561 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1621 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1681 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1741 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1801 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1861 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1921 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1981 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2041 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2101 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2161 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2221 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2281 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2341 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2401 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     2461 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     2521 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     2581 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     2641 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     2701 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     2761 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     2821 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     2881 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     2941 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3001 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3061 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3121 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3181 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3241 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3301 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3361 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3421 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     3481 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     3541 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     3601 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     3661 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     3721 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     3781 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     3841 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     3901 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     3961 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4021 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4081 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4141 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4201 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4261 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4321 caggatgaac gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa atattaatat
     4381 acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata tagaaccgtt
     4441 tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc aaaagccgga
     4501 ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc tatttgatga
     4561 ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt acctaaaggg
     4621 cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg acatagttcc
     4681 gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg attgctgcaa
     4741 tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg aaattttatt
     4801 tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga tatcatggaa
     4861 ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt ttattgaaag
     4921 gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg attcctatga
     4981 aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat ccggtgaggt
     5041 ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa aatataccag
     5101 ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat atgccgctga
     5161 tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct gaaagatatc
     5221 gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag
     5281 aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta ccaagacgaa
     5341 gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata
     5401 atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg
     5461 aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata
     5521 ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta
     5581 atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc
     5641 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
     5701 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     5761 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     5821 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     5881 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     5941 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     6001 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     6061 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     6121 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     6181 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     6241 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     6301 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     6361 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     6421 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     6481 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     6541 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     6601 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     6661 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     6721 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     6781 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     6841 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     6901 cagagtaacc tctattctta ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat
     6961 tgcacagcga tttaatataa gcttaaggac agtttacaga gatatccgga cacttgaaga
     7021 agccggaata ccaattggat ctgaggccgg aaaaggatat tttcttgtag agggcttcct
     7081 acttccgccg gtaatgttta cagcggcgga agtgggtgca ttgattacag cagggaaatt
     7141 tttaaattgc catggagatg aatcatttat aaaggatttt gattcagcta tgtataaaat
     7201 caaatctatt ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga acagtattaa
     7261 cgtgtacagc acatctggac agaaaaatac attggcggat aacgttattg cagcaattca
     7321 gactgcaatc tgcaataaaa gggtaatatc aattcaatat cctgcatcag gagggcaaga
     7381 accggaaagc agaatgatag a
//
LOCUS       DACEJR010000106         7187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEJR010000106 DACEJR010000000
VERSION     DACEJR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.112"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW651_002670"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW651_002670"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0815344.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW651_002671"
     CDS             complement(1172..1867)
                     /locus_tag="IW651_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0815345.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW651_002672"
     CDS             complement(1880..2596)
                     /locus_tag="IW651_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0815346.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IW651_002673"
     CDS             2926..3534
                     /locus_tag="IW651_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0815347.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IW651_002674"
     CDS             3859..4182
                     /locus_tag="IW651_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0815348.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IW651_002675"
     CDS             4364..5734
                     /locus_tag="IW651_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0815349.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IW651_002676"
     CDS             6081..>7187
                     /locus_tag="IW651_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0815350.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACEJR010000107         7015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEJR010000107 DACEJR010000000
VERSION     DACEJR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.113"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..455)
                     /locus_tag="IW651_002677"
     CDS             complement(3..455)
                     /locus_tag="IW651_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0815351.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            605..1555
                     /locus_tag="IW651_002678"
     CDS             605..1555
                     /locus_tag="IW651_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0815352.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1548..2507
                     /locus_tag="IW651_002679"
     CDS             1548..2507
                     /locus_tag="IW651_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0815353.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2504..3259
                     /locus_tag="IW651_002680"
     CDS             2504..3259
                     /locus_tag="IW651_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0815354.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3282..4235
                     /locus_tag="IW651_002681"
     CDS             3282..4235
                     /locus_tag="IW651_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0815355.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4879..5175)
                     /locus_tag="IW651_002682"
     CDS             complement(4879..5175)
                     /locus_tag="IW651_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0815356.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5531..5887
                     /locus_tag="IW651_002683"
     CDS             5531..5887
                     /locus_tag="IW651_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0815357.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5897..6796
                     /locus_tag="IW651_002684"
     CDS             5897..6796
                     /locus_tag="IW651_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0815358.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         2132 a         1204 c         1420 g         2259 t
ORIGIN      
        1 ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt ttcaactggc
       61 gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt tactgcttct
      121 tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc gttgactaca
      181 gtaaatcggc tattgttttt cgttgctagc tggatatatt ggttttggac agaaaggggt
      241 agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt taaaaagttt
      301 tctttgtttt ttggttggat catttcagca acagtcatac taagatagca gcgttccctg
      361 aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc ataaagtccc
      421 ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct tcttctcttt
      481 gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa aaagcggttg
      541 cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta gaaaggtgga
      601 ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta tttcattgtt
      661 catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc aaagcttaca
      721 aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg gagcgaccag
      781 tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt caccaacgac
      841 tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt tcctacctgg
      901 tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag gaacattgct
      961 ttttttatcc cttacgcgat tgttccccca gaaaaatcaa ttgatgcttc cgttgattgg
     1021 agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc aattccagtt
     1081 ggtacaaaat atgacttcat ggcttcaagg aaattttgcg actgtgatac gaggaaatta
     1141 tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg cttaccgatt
     1201 tacgatcatt ggtctgggcg aatcgtttgc agtaaatttg ggaatagatt accagaagat
     1261 gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga tcatggtagg
     1321 aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat atggagatca
     1381 agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag tattttgcga
     1441 tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga tcgttggaat
     1501 catcggtggg gctatcttcc tttatcttct gataaggggg cgacgatatg cttaaaaaaa
     1561 agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga atgtacttga
     1621 cgatccatac atatggaaac tggcattttg catgggcatt gaggagtaag aaaatttttg
     1681 catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag acaattacgc
     1741 ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc cttgtccaaa
     1801 cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt acagtttttt
     1861 tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc tttcttttga
     1921 aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta ggtacgcttt
     1981 ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat gatttgcttc
     2041 aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg cctgcttctt
     2101 tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta gacgttttcc
     2161 atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt gaatttttga
     2221 ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt ccagttactt
     2281 ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat cgtcatggtc
     2341 agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga cagctcgcag
     2401 tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt gtcggaggaa
     2461 tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg atttacaaaa
     2521 ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa cgattcctga
     2581 aaagaagttg actgctttta tcggtccaaa tggtgctgga aagtcaacgg tgttgtcgat
     2641 gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata acgaagtaaa
     2701 ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat cgaatgaaat
     2761 aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt atacgaaagg
     2821 aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacacc ttgggttaga
     2881 agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac gtgcgtatat
     2941 tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt tgaacaatct
     3001 agatatgaat tttgcggttc aaatcatgca gatcttaaaa aaattagtac aagaaatcgg
     3061 gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg cagacgaaat
     3121 tattgcaatg aaagatgggc agttattcac gcaaggaacg acccaagaaa tgataaaaaa
     3181 agatgtactg gacgaattgt acgaaatgga catacgaatt tgtgagattg aaggtcgcag
     3241 attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa ttagtagtag
     3301 gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagtgac aacaccagtc
     3361 aggcagatac aaagaaaagc agtacgcaag ctgaaacggc attaacgata aaagacagta
     3421 atggcgatca aatcgaggtg ccaaagaatc ctgaaaaagt cgtcgtattc gacaatggtt
     3481 ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca gctacaagca
     3541 gccttcctga gtatctttca tcctataaaa aagtggaatc agcaggtggg atcaaagaac
     3601 cagacttaga aaaaatcaat cagcttcagc cagacctgat catcatttct ggccggcaaa
     3661 gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct ttagatgcga
     3721 aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc tttggtaagc
     3781 aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag acgaaaaaga
     3841 aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg caattatcag
     3901 cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt gaacaagcag
     3961 atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat gtgctggaaa
     4021 aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga gacgacagca
     4081 aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa aatcagcaga
     4141 tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa tcaatgaaac
     4201 tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg attaaactct
     4261 catactatgt aacaataatg tttcttacat tcttaaatag caagagccta tcctcaaaga
     4321 agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt tttatttatt
     4381 tgggaaatct tttttcaacg tgaattcatt ctctacatca tacgggttga ttctagaatg
     4441 gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct cctttgattt
     4501 gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt ttattttgta
     4561 ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt ccactagact
     4621 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt
     4681 gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag tccactagac
     4741 tagaaaatat gagaagctat tttctgtcca gcgttcttat tacttaaagc aaatcgtagt
     4801 tgtcacaatc tcttctaaac gatgttccat cagctgacct tcggtattag ctcaaagcac
     4861 tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt tgatgatctg
     4921 catttttcga taaaaatcgg cgggaagctt actccaccgt tttaataatt gatagaaata
     4981 actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat tcgttttttt
     5041 tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga caaagagtgt
     5101 ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat agatgttttt
     5161 tatatattcc ttcatagtga ttcctccaaa gaagtctttt tatttgataa gtttactcta
     5221 ccgaagagtt gttaataatc cgtgaacaaa aagtaattct ttttccaata gttgttaaag
     5281 gttttttatt ttttatagta ttttttgtta tttaagaaag gaataaggtt aaacaaataa
     5341 caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt
     5401 ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa
     5461 cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga
     5521 ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt
     5581 agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag
     5641 ccaagtcagt tgccaattgg acacggcatt tcgattgatc attgtcaaaa aagatacaca
     5701 aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa
     5761 agacagtgca atactttaca tggatgatcc atccacactt gctttagaac caacttataa
     5821 tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa
     5881 agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa
     5941 gaaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac
     6001 tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga
     6061 atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga
     6121 tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta
     6181 tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt
     6241 ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg
     6301 gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa
     6361 aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt
     6421 tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgctgga acaaattaaa
     6481 tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca
     6541 agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta
     6601 cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa
     6661 agaacagctg ttagatagct atgaacccat gaaattacgt gtcctcttgc gtgcccatca
     6721 tccggccttt caagcctatc aaacacaaca tttgcaagag gaatttgaac aggcgatgac
     6781 aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata
     6841 aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt tgtggaaaat
     6901 aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg
     6961 tttattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt tttat
//
LOCUS       DACEJR010000108         6983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEJR010000108 DACEJR010000000
VERSION     DACEJR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.114"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1050
                     /locus_tag="IW651_002685"
     CDS             <1..1050
                     /locus_tag="IW651_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ0815359.1"
                     /translation="IKKNMEVNQIFRRYVADEDLLIYSIDESILKVTRSLNLFTTEGT
                     RSQRRKKLAQMIQGHIKEETGLIATVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENV
                     PEKVWNIKEMTDFWGIGLRMKKRLNHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHA
                     NGIDRTDIAIPPEPTKEKSYGNSQVLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKT
                     GCVRLAIGTSILEEKSGFSHQMKIPITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYS
                     RLVYTDSLQLDLFSDPRKKIAEENLDRIIDKIRQKYGFTAIIRASSMLEGARSIKRST
                     LIGGHAGGLGGLHND"
     gene            1043..1390
                     /locus_tag="IW651_002686"
     CDS             1043..1390
                     /locus_tag="IW651_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815360.1"
                     /translation="MIEYNDRGRLKWVGFYLSDHTERINSNNVQRANHNSAKEQMTTE
                     EIAKVLKTAVIKNRPVCIQKEERNAEGYYPDDIVGKIIGSDELGVFVEEEKVHYDEIR
                     NVSFTELVKWSQI"
     gene            2814..3581
                     /locus_tag="IW651_002687"
     CDS             2814..3581
                     /locus_tag="IW651_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0815361.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3607..4152
                     /locus_tag="IW651_002688"
     CDS             3607..4152
                     /locus_tag="IW651_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815362.1"
                     /translation="MGERTQALITVKYGNSKVKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKVYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            4153..4719
                     /locus_tag="IW651_002689"
     CDS             4153..4719
                     /locus_tag="IW651_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815363.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            4734..4982
                     /locus_tag="IW651_002690"
     CDS             4734..4982
                     /locus_tag="IW651_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815364.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMLGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAEYIFSWLGQ"
     gene            5002..5241
                     /locus_tag="IW651_002691"
     CDS             5002..5241
                     /locus_tag="IW651_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815365.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKAQRKGYPDKIFKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            5258..5761
                     /locus_tag="IW651_002692"
     CDS             5258..5761
                     /locus_tag="IW651_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815366.1"
                     /translation="MNEDKMVLSEYARLRTINWLNGMQSSGAFDGFDEEVEDEQELIL
                     ERQRILRSEQDSKILYGLYDLQLNGLKAQYVADSFDDMVEQGADRVVTYSEDDEEEWE
                     NYSDDEICFNDGYMPIEITKEEYDKVSDSDEIGMCTVVSGLDIDVQRVEVAYEKMKER
                     LKSKVES"
     gene            5868..6149
                     /locus_tag="IW651_002693"
     CDS             5868..6149
                     /locus_tag="IW651_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ0815367.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFNEA"
     gene            6139..6480
                     /locus_tag="IW651_002694"
     CDS             6139..6480
                     /locus_tag="IW651_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0815368.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSEQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAISKSRENISFKKVGMLSASDTKKL
                     NRFIERLQRKV"
BASE COUNT         2616 a          944 c         1473 g         1950 t
ORIGIN      
        1 ataaaaaaga atatggaagt taatcagatt tttagaaggt atgtagcaga cgaagattta
       61 ttgatatatt cgatcgatga atcgatccta aaagttactc gatcactgaa tctttttacg
      121 actgaaggaa caagaagtca gcgaagaaaa aaactcgctc aaatgatcca aggacacatt
      181 aaagaagaga ctggattaat cgctacggta ggtgtcggcg ataatcccct tttggctaaa
      241 ctagccttag acaatgaagc aaagcataat gaaggatttg ttgcggaatg gacgtacgaa
      301 aacgttcctg aaaaagtatg gaatatcaaa gaaatgacgg atttttgggg tattggatta
      361 cgcatgaaaa aaagattgaa tcacatggga attatgagta tagaagattt agcaaattgg
      421 aatccctaca caattaaaag tcagttaggt gtaatcggac ttcagttata tttccatgcc
      481 aatggaattg atcgtacgga tattgctatt ccaccagaac caaccaaaga aaagtcttat
      541 gggaattcac aagttctacc aaaagattat acgaggagag aagaagtaga actagtagtc
      601 aaggagatgg cagagcaagt agccattcga attagacaac ataattgcaa aactggttgt
      661 gtacgcttag caattggtac gtcaattttg gaagaaaaat caggtttctc gcatcagatg
      721 aaaataccaa ttacagataa tacgaaagaa ttacaaaatt attgtctttt tttattcaat
      781 aagttttatg aggggcagga agtacgacat ataggagtaa cttatagcag attagtatac
      841 acagattccc tacaattaga tttatttagt gatccaagaa aaaagattgc tgaagaaaat
      901 ttggatagga ttattgataa gattagacaa aaatatggat ttacagctat tatacgtgct
      961 tcatctatgt tagaaggtgc aagaagtatt aagagaagca ctttaatagg aggacacgca
     1021 ggaggattag gaggtttgca taatgattga atacaacgat agaggacgtt taaaatgggt
     1081 aggattttat ttgtctgatc atactgaaag aatcaattca aacaatgtac aacgtgccaa
     1141 tcataactcg gcaaaagaac aaatgacgac tgaagagatt gcgaaagtac tgaagactgc
     1201 tgtcataaaa aatagacccg tctgcattca aaaagaggaa cgaaatgctg aaggttacta
     1261 tccagatgat atagtaggaa aaattattgg aagtgatgag cttggtgtat ttgtggaaga
     1321 agaaaaagtc cactatgatg aaattaggaa tgtttctttt acggaattag taaaatggag
     1381 tcagatatga acattattaa gaaatacgaa ttgggttatg ttacctatga agagctaata
     1441 gaggaaatat ggggatatgg tcaacaatta attaatcaag tggggattga ttgcttttgt
     1501 ttctatattg aatccggagc aggataccat aagaacagat actatatatt tccatatcca
     1561 ccaaaatagt ctcttagaga tttttaaatt ttctccaaat actttcaatt attgtgaaat
     1621 atttccctta aaacaaatag taaaagctat gaatcagctc agaaagcatt aaaatctgaa
     1681 gaagagcaat tttttacaga agaggaattg gataagatgt taccagagca tcttagaaaa
     1741 aagagttcta aaaattgaac tcttttttta tgcttaaaat tctcttgtaa tttttctatt
     1801 caatcaataa ataatctata tttaagaaga aatatgataa tagaacatct cctaaagcca
     1861 ccgcaagccg tcttaagcat agcttagccc gacattgcta gattagtgca tctcgccata
     1921 aaagttttct aaagccaccg caagccgttc taagcatagc ttagcccgac attgctagag
     1981 atatgctcat ctcaccatga acgtttccta aagccaccgc aagccgtcct aagcatagct
     2041 tagcccgaca ttgctagata tgctcatctc accatgaacg tttcctaaag ccaccgcaag
     2101 ccgtcttaag catagcttag cccgacattg ctagattagt gcatctcgcc ataaaagttt
     2161 tctaaagcca ccgcaagccg ttctaagcat agcttagccc gacattgcta gatatgctca
     2221 tctcaccatg aacgtttcct aaagtcaccg caaaccgtcc tagccatcgc ctagtccgat
     2281 tttgcttgat cggttccatt tctgtttatt tagtgggtca acaccgtaat cttaaatagc
     2341 catcgcctat ttaagattac taggtctgct ttgaaggtaa aaagtctttc attcttcaag
     2401 acttttaagc tttcaagcta cgcctgaaag cattgaaatt catcgctctt tatttggttt
     2461 tgccatttat aattccttac gtccattatc tttcttgcct gtcatgcctt gtcagaagtg
     2521 tttagagctt atctcatctt tggattaata caagtggcgt aaattttttt ctaaacgcaa
     2581 gcgttacgaa aaaaatttac ttaaacactt gtattaatcc ggcagataag aacgcattct
     2641 aatcactccc gacaaggcaa gacaggcgaa aaagataaat aaatgaacga aaggaagaaa
     2701 cataaaatgc aaaaccaaat agcgatgaag aaaaaacctc ctccctctaa aacaatccaa
     2761 acaaaaaaga attttgaaaa accaaaaatc aaaaacagaa agaagcgttc aacatgagag
     2821 atagaatttt agaagtaaat caagaaatgg taaacaagtt aacaaatatc agtggaaact
     2881 atgaaaagta cgatggtact ttacttgata actatatttt tcacgataca caaaatatca
     2941 gaattggcag aagtaaaccg agagcgttca ttctcatcaa agagaaatat ttgaatgaat
     3001 ggaatagcac ctatgaaatg gttatgactg ataactttga aaaggtattg gatttcagaa
     3061 atgctttgtt gggttctgat atgggaaata gcagtcttga atctattgaa gttgaaaaca
     3121 agaccgagaa tgaaggtgaa ggattgatta agaatgtcct gaaaatcgaa caaaaatata
     3181 tcagtgaaga tagaagtttt tatagagtca caagctcggt tggtctagca aactttgcta
     3241 ctaacttaat tggaaacaat gctagtgaat ctttgctagt gatgtgtctg aatacaaaga
     3301 atgaggtcat tgcttatagc gaagttttca aaggtggaat taatcaatca gtagcgatac
     3361 caagagagat ttttcaacta gctttgctaa acaattcagc aaaaatcgca gtatcacata
     3421 atcatccgag cggatatgaa tttccaagtg aaaatgacaa aagttttact acgaatttga
     3481 aaaacgcagg aaaaacaatt ggtattgaat tgttagacca tatcatcgta acaagtaaaa
     3541 acacaagcta ttactcattt caagaaaatg gttctttata aaaattagag aaaagaggaa
     3601 aacaagatgg gcgaaagaac acaagcgtta attacagtca aatatggtaa cagcaaagtg
     3661 aaagcaagcg tgtcaattca ttatcagtgg ggatatggtc gatacatgtt gatggacgtt
     3721 ttgaatcaag gaatatattt aatcaataaa gtatatgact tacgagatga atcaagagaa
     3781 gaattagcaa aaggaatctt tgatagaagt acaggagtta tcaattttgt cacctatgaa
     3841 tctggagtga gtgcttatga tgaaacaggg tttggtgggc gctatgaacc ttatcaagaa
     3901 acgattgaag atgtgatgaa gttttcagat aacaatgatg gctatgctca tttagagatg
     3961 gtgttcgatg aaaatggatt tagtctagat gataaaacca gattgcgatt gtttgatttt
     4021 gagggtcaac agataggact aaacaagttt tgtgaaagcg gaaaagagtt tgctacgaaa
     4081 gattttatta agggatacag attattgcta gaaaattatg gaataaacct agaagaagag
     4141 agcgtgaaat aagtgtttga aaaaagaaaa gaccagttcc ctaatttaga agcgaaaagt
     4201 caaaaagtga ttgatggtga aatgattgaa ttattccaaa aaagttatgg aaccatagaa
     4261 actaccgcaa gttgcaaata ctactttcaa ggaaagagaa acacgtttaa aggctttgcc
     4321 gattctgatg aagaattggc aaagaaaatc attgattggc ttgtcagcag tgaacacgta
     4381 gcaagagaag ataccgttag aacaacgatt aatggggaag tcctaacagg tactagatgt
     4441 gtttgttgta atcatgaatt tgtagaagat gaaggttgct accgaatcaa tgaatatggt
     4501 gattttattt gccagagttg ccacgagata gaatttccaa ccgaaagaag ttggtttgaa
     4561 tacatgctac aagattatga gatggaaaaa gaaaatggtg aagtagtttt ttgtaccaaa
     4621 gaggaattga gtaagagaag cgatgatgaa attgaagatt ggtgtgtgtg gttagtaggg
     4681 gcagatgatt gcgttgcata ttacacaaca gcagaatagg agtgtgaatc agcatggaag
     4741 gaatggattt tctagatcat gaagatttgg ttgattttgg ttatacatgg aaaggaatgt
     4801 taggtattag cagaagtctt gctaatgcct tttacgaaag aaattatgca gtatatgttc
     4861 tttatgatga cgatacagaa agccttgttg acgaggaata taaattggat ttagagaatg
     4921 ttttatatgg aatagaaaag gaagatttag cagaatatat tttttcatgg ttgggtcaat
     4981 aagtgaaagg aagatagaaa catgacaaaa tttaaaagta agggtatgca atggtgttct
     5041 tattattatg ttggaaatga tgaagattta ttgagaaaag cccaacgaaa aggttatcca
     5101 gataagatat tcaaacaaat aggttattac atgccagaag agaggataga aaaagaattt
     5161 tgtgaggttc tttttgatag tgaacagcta ttagttttgt taacacaaaa taaattgatt
     5221 tgccaatttg aaaataatta agatgaggtg gtgaaccatg aatgaagata agatggtatt
     5281 aagtgaatac gctagattga gaactattaa ttggttgaat ggtatgcaaa gtagtggggc
     5341 ttttgatgga tttgatgaag aggtggaaga tgagcaagag ttaattttag aacgtcaaag
     5401 aattttacgc agtgaacaag atagtaagat attgtacggt ttatatgact tgcaactgaa
     5461 tggattaaaa gcgcagtacg ttgctgatag tttcgatgat atggtagaac agggggcaga
     5521 tagggttgtc acttattcag aagatgatga agaggaatgg gaaaactatt cagatgatga
     5581 gatatgcttt aatgatgggt atatgcctat tgaaatcacc aaagaagaat atgataaagt
     5641 gtccgatagc gatgagattg gtatgtgtac ggttgtaagt ggattggata tagatgtcca
     5701 aagagtagag gttgcatacg agaagatgaa agaacgttta aaaagtaaag tagaatcttg
     5761 atttgattgg attgtttgca aagtagctaa ttaagagtat aatataatca taaatgtagt
     5821 taattgatat aataatgagt atattatact caaaagaggt gaacgaaatg aaggtcaaaa
     5881 atccaaaaga aaaaagcaga attcaagtcg gaatagacaa ggatttgaaa gaaaatgcag
     5941 aaatgatatt ggaagagcta gggttaaatc caacaacagc aatcacaatc ctttataagc
     6001 aagtggtcgc tagaggtgag tttccagtag agataaagct aagcgaagag gaaaagcaag
     6061 gaatcagatt gcaacagcta actaaggata tgccagttga tgttctagat acagatgaaa
     6121 agttagaaga gtggttcaat gaagcctaat gagatagtaa cggtatatgt tgcatttaca
     6181 gataaaaatg gtggtaaaag aagacctatt ttggtggtta gtgataaaga agatagagta
     6241 gaattctttg gaatcacaag tcaatatgag aaaaaatcag agcaaataaa gagggtgtat
     6301 tttcctattg cagaatggga aaaagcagga cttaaaaagc aatcatggat tgacgtaggg
     6361 tctttaaagg ctatttccaa aagccgagag aacatatcat ttaaaaaggt tggtatgcta
     6421 tcagctagtg atacgaaaaa gctaaatagg tttatcgaga gattacaaag aaaagtctaa
     6481 caaggctttt ctttttttgc ttatatgtat attaagatat acgtctatat aaacgtattt
     6541 atgtatataa aaataaacgt aaatatatac gtgaatataa atattaagaa aatattaaga
     6601 attgttaaat attgataatt atcaatatac gaattataat gtatatttta tatcaaatgt
     6661 atgtaaataa ataaaatgac tgacggatat tgaatagtga taaataacag taatccagac
     6721 ggatacagtg agggaagtag cttgctacga acgaacaggc agaatgaaac ggtagtttca
     6781 ttagagcaag tcgcttgcga catagcgtta ccactgatgt aactggcaag ttacatagag
     6841 agagtttgct tgcaaacgaa cgttacctac ttgaatagaa atcaaaaata acagattagt
     6901 gaattttgaa tagtagccac aactcaccca accgcttgcg gtgggtgagt cccacggagg
     6961 aaaagacgaa cgaacggtcg cgt
//
LOCUS       DACEJR010000109         6834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEJR010000109 DACEJR010000000
VERSION     DACEJR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.115"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..859)
                     /locus_tag="IW651_002695"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IW651_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IW651_002696"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IW651_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0815369.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IW651_002697"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IW651_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IW651_002698"
     CDS             complement(2143..2373)
                     /locus_tag="IW651_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0815370.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IW651_002699"
     CDS             complement(2603..3421)
                     /locus_tag="IW651_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0815371.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IW651_002700"
     CDS             3582..4271
                     /locus_tag="IW651_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0815372.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IW651_002701"
     CDS             4285..5787
                     /locus_tag="IW651_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0815373.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IW651_002702"
     CDS             5800..6276
                     /locus_tag="IW651_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0815374.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IW651_002703"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IW651_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2220 a         1075 c         1246 g         2293 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgtatggaa tggcaacagt
     6661 ttttttgaca aattttataa ggtgcagaac ttctttccgt atgctattcc gattgtcctt
     6721 gacaatgagc ctcctcggat gtgacgatct tcggcaggag ctaacagtta aagttagact
     6781 gcctcgctag cgttagcaca tccgagctca ttatcaagga ttcgctgcgc caat
//
LOCUS       DACEJR010000110         6803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEJR010000110 DACEJR010000000
VERSION     DACEJR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.116"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..926
                     /locus_tag="IW651_002704"
     CDS             36..926
                     /locus_tag="IW651_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0815375.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(999..1823)
                     /gene="nadE"
                     /locus_tag="IW651_002705"
     CDS             complement(999..1823)
                     /gene="nadE"
                     /locus_tag="IW651_002705"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0815376.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1835..3307)
                     /locus_tag="IW651_002706"
     CDS             complement(1835..3307)
                     /locus_tag="IW651_002706"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0815377.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3944..4306)
                     /locus_tag="IW651_002707"
     CDS             complement(3944..4306)
                     /locus_tag="IW651_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0815378.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4529..5281
                     /locus_tag="IW651_002708"
     CDS             4529..5281
                     /locus_tag="IW651_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0815379.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5421..6344)
                     /locus_tag="IW651_002709"
     CDS             complement(5421..6344)
                     /locus_tag="IW651_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0815380.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6359..6631)
                     /locus_tag="IW651_002710"
     CDS             complement(6359..6631)
                     /locus_tag="IW651_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815381.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2082 a         1352 c         1162 g         2207 t
ORIGIN      
        1 aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata aacgaagaat
       61 ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc cagctacttt
      121 ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc agtgctatca
      181 cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa aaagtaggac
      241 aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat cagttttcca gaaaaaatat
      301 tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca aattatgcaa
      361 ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg gatgaaccac
      421 gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc agcggatttg
      481 tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg ttggaagttt
      541 ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca gatatcactt
      601 tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt gctgatatcc
      661 gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc ttgctgaaga
      721 cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca aacatcacga
      781 tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg aaagacgatg
      841 ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac gatcaggata
      901 tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa acaaaaacag
      961 ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa atcatccaaa
     1021 acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc gattttttct
     1081 tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt atatgtgaca
     1141 cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc agtcggaatt
     1201 tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc ttgtcgtttg
     1261 tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt gaagaatcca
     1321 gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc tccagctacg
     1381 gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt
     1441 cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt gatatttact
     1501 cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc ttcatctgct
     1561 tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt tgtttcttca
     1621 cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg
     1681 ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc tttcataaag
     1741 tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc taattcattg
     1801 ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga agttctgcta
     1861 cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca gtagacagat
     1921 cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt gaatctagat
     1981 tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt
     2041 cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt ttattgataa
     2101 acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct tcatcccata
     2161 atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc cacacttgtt
     2221 ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta tccatcattt
     2281 ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct ggctgatcat
     2341 aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt tgcatcttta
     2401 aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct tccgtaaatc
     2461 cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct ccactgtcga
     2521 tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga atcgcacttg
     2581 gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct ttatgggttt
     2641 tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac gaatgagcat
     2701 gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt gttgcatctg
     2761 ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct tgtgcccgtc
     2821 tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga gctgcttttg
     2881 tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact aattggcagt
     2941 gcgctaaagg cccttccact tggattaatg gttcatttgc aaaaactagt tctccttcac
     3001 gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa aaaccttcag
     3061 gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc agattctcta
     3121 aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt
     3181 ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc cagtatgtct
     3241 gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct gggtaagtat
     3301 tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta taacaaaaaa
     3361 aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt acggatattt
     3421 cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat ttaacctaaa
     3481 cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta gcgttttcta
     3541 tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg ttaagatacc
     3601 gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa aagtttttga
     3661 agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat ataacttatt
     3721 atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac
     3781 tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca gctgtcaata
     3841 gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta ttgtatacat
     3901 tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata gaatccttcg
     3961 tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt attccggcac
     4021 gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc atttttaaaa
     4081 cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta ttgatcatca
     4141 cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct aacgaaaata
     4201 atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta ggataaaatg
     4261 tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca ttcctctttt
     4321 ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta gcattaagga
     4381 atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact taatagtata
     4441 acataatttc gtgagaaaaa aatacaattt tttcttgacc atctcgttac gtaaaggatt
     4501 atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa tgtcagagat
     4561 atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc taaaaccagc
     4621 taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca
     4681 catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg tcttagacaa
     4741 tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc tggaaaaacg
     4801 ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt acgaaggagg
     4861 aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa
     4921 tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg ttacagcatc
     4981 caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa
     5041 gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa gcttagcaga
     5101 ccgtatcttc caagctcata agaagtggtt ctcttacagc tggcctgaat atcagccaaa
     5161 agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg catattatga
     5221 cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta
     5281 atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca aaacagcaga
     5341 cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc acaagttttt
     5401 tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt cactttatcc
     5461 tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag atggtggtcg
     5521 atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat caccactggt
     5581 gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga atttgtaaag
     5641 acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt ctctgcatct
     5701 atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg acatagctgc
     5761 aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat gcccaattgc
     5821 tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc tcgatcaata
     5881 aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg tccatatttt
     5941 tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa tccatcgaaa
     6001 gctttagctg caaattcttc aagatacctt cgctcttttt ctttatgata gcccgtcggt
     6061 aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact gatcaactga
     6121 tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt ttttccataa
     6181 cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac cgcaagtatt
     6241 tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga aatagtagaa
     6301 actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc ttcgccacct
     6361 atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca aaaaactgat
     6421 acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata tcttttgtca
     6481 aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac ccttgaaaac
     6541 tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa ttgttcgttg
     6601 tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat actttgagta
     6661 tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa gttttattcc
     6721 tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg atactgtaaa
     6781 tatatcttta ttattagtga atg
//
LOCUS       DACEJR010000111         6701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEJR010000111 DACEJR010000000
VERSION     DACEJR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.117"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW651_002711"
     CDS             <1..1023
                     /locus_tag="IW651_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0815382.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IW651_002712"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IW651_002712"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0815383.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IW651_002713"
     CDS             3389..3985
                     /locus_tag="IW651_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0815384.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IW651_002714"
     CDS             3998..4897
                     /locus_tag="IW651_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0815385.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IW651_002715"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IW651_002715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0815386.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IW651_002716"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IW651_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0815387.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IW651_002717"
     CDS             5430..5663
                     /locus_tag="IW651_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815388.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IW651_002718"
     CDS             complement(5822..5944)
                     /locus_tag="IW651_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0815389.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IW651_002719"
     CDS             6134..6358
                     /locus_tag="IW651_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815390.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT         2394 a         1187 c         1418 g         1702 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt g
//
LOCUS       DACEJR010000112         6452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEJR010000112 DACEJR010000000
VERSION     DACEJR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.118"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..550)
                     /locus_tag="IW651_002720"
     CDS             complement(95..550)
                     /locus_tag="IW651_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815391.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="IW651_002721"
     CDS             complement(704..1912)
                     /locus_tag="IW651_002721"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0815392.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="IW651_002722"
     CDS             complement(2024..2356)
                     /locus_tag="IW651_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0815393.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="IW651_002723"
     CDS             2504..3379
                     /locus_tag="IW651_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0815394.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="IW651_002724"
     CDS             complement(3461..4042)
                     /locus_tag="IW651_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0815395.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="IW651_002725"
     CDS             complement(4048..5307)
                     /locus_tag="IW651_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0815396.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="IW651_002726"
     CDS             complement(5309..6355)
                     /locus_tag="IW651_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815397.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1798 a         1334 c         1100 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaata aa
//
LOCUS       DACEJR010000113         6381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEJR010000113 DACEJR010000000
VERSION     DACEJR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.119"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(312..842)
                     /locus_tag="IW651_002727"
     CDS             complement(312..842)
                     /locus_tag="IW651_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0815398.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="IW651_002728"
     CDS             complement(1024..1680)
                     /locus_tag="IW651_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0815399.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IW651_002729"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IW651_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0815400.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="IW651_002730"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="IW651_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="IW651_002731"
     CDS             complement(2948..4162)
                     /locus_tag="IW651_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0815401.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4503..4745)
                     /locus_tag="IW651_002732"
     CDS             complement(4503..4745)
                     /locus_tag="IW651_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0815402.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4777..5334)
                     /locus_tag="IW651_002733"
     CDS             complement(4777..5334)
                     /locus_tag="IW651_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0815403.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5347..5535)
                     /locus_tag="IW651_002734"
     CDS             complement(5347..5535)
                     /locus_tag="IW651_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0815404.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IW651_002735"
     CDS             complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IW651_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0815405.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1895 a         1339 c          953 g         2194 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactct
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     4381 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     4441 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     4501 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     4561 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     4621 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     4681 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     4741 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     4801 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     4861 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     4921 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     4981 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5041 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5101 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     5161 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     5221 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     5281 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     5341 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     5401 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     5461 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     5521 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     5581 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     5641 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     5701 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     5761 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     5821 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     5881 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     5941 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6001 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6061 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6121 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     6181 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     6241 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     6301 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     6361 gttattatta caagattcta t
//
LOCUS       DACEJR010000114         6055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEJR010000114 DACEJR010000000
VERSION     DACEJR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.120"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            822..1556
                     /locus_tag="IW651_002736"
     CDS             822..1556
                     /locus_tag="IW651_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0815406.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1572..2741
                     /locus_tag="IW651_002737"
     CDS             1572..2741
                     /locus_tag="IW651_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0815407.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2757..3752
                     /gene="lacD"
                     /locus_tag="IW651_002738"
     CDS             2757..3752
                     /gene="lacD"
                     /locus_tag="IW651_002738"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0815408.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3831..4757
                     /locus_tag="IW651_002739"
     CDS             3831..4757
                     /locus_tag="IW651_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0815409.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4776..6026
                     /locus_tag="IW651_002740"
     CDS             4776..6026
                     /locus_tag="IW651_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0815410.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2023 a          995 c         1242 g         1795 t
ORIGIN      
        1 cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta actttaactg
       61 ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac aatcggaata
      121 gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt gccattccaa
      181 agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt gactaaacga
      241 agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat tcagataaac
      301 tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc ttaagagaga
      361 acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat ttattctcat
      421 agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga tttgaaacca
      481 aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca ctggtaagta
      541 gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta aaaaaagata
      601 ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa aagttggaaa
      661 attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat attaacaaga
      721 ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa ataccactac
      781 tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat acctttcaat
      841 caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa aacagtatgg
      901 tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc gttagtcgca
      961 cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat cgacaacatg
     1021 gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct ggcgcatata
     1081 gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata ttggccttca
     1141 agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta ggtgagcctt
     1201 tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc gagaaatcct
     1261 atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg aaacctttat
     1321 ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag gaattttatg
     1381 ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga tcaccagtgc
     1441 tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt acattaagtg
     1501 tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact aactgaattg
     1561 gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga gcagaaaata
     1621 ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac cgttatcaag
     1681 aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct ggagagcgac
     1741 caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat acactaattc
     1801 cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt ggttcgaccg
     1861 atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc ttggtctcat
     1921 ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc aatcagctgg
     1981 tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg gctacacaaa
     2041 tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat gacgctggtt
     2101 ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt tttgatcaag
     2161 caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga gaagaagtca
     2221 tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc atcatttact
     2281 taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt ctcgagttaa
     2341 ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat ggtcccaaat
     2401 cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca tatacgcaga
     2461 aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa cgcgttgttg
     2521 gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt gcagattcgt
     2581 tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg atcgcattac
     2641 tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga acagtcaatc
     2701 gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg tgaagcatgt
     2761 tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca gaaggcatta
     2821 ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg gctttaggga
     2881 tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa gaattgactc
     2941 cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca cagaagcgtg
     3001 cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa accgtacccg
     3061 gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag ttaggtgcgg
     3121 atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc aatcggcaaa
     3181 agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg ccattttatt
     3241 tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc tatgccaaag
     3301 caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag tataaagtag
     3361 atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat gcaacagggg
     3421 agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc gatgcaacag
     3481 atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg gagacattgc
     3541 gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga gcaacttgga
     3601 aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa tggctgacag
     3661 aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact gccatcccat
     3721 ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt agtgataaga
     3781 gatatgtgtg attataattt atcattattg aaactgggag gatattttat atgaataaaa
     3841 gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa ttaaagcagg
     3901 caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt agctgtcctt
     3961 acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct gatttaattt
     4021 tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta actctaaaag
     4081 agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta aatttagaac
     4141 ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt ggtagaatcg
     4201 tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt tgtttaactg
     4261 gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca ttatccaaat
     4321 caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt gatgaaccaa
     4381 ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt ttattagttc
     4441 ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc gttgattata
     4501 taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta acggctaatg
     4561 gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta gcatcaaaag
     4621 catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt cttccagaaa
     4681 atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt atgttaggaa
     4741 aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt caaaaaatag
     4801 ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg gctgcagtta
     4861 tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg atagaaggat
     4921 ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca tcagattcaa
     4981 catttggtat tattggatta gctgttacct actctttggg acacaatcta gcaaagactt
     5041 atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt ttacttacac
     5101 ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt acctcgggtc
     5161 tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg tttgtactta
     5221 aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga gctttcgttg
     5281 ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta atttgttata
     5341 aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa ccactatctt
     5401 tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt ttcttttgga
     5461 tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta ttattgcaaa
     5521 actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac attataactt
     5581 atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt gcacttgcca
     5641 ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag ttatcaatcg
     5701 ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact atattaaata
     5761 taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt acttattttt
     5821 caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg acaactccac
     5881 cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg attcaagtaa
     5941 taacaattgc aataaatgtt ttgatatact atccattctt taaattagat gataatgcca
     6001 agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt agtta
//
LOCUS       DACEJR010000115         6051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEJR010000115 DACEJR010000000
VERSION     DACEJR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.121"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            30..1331
                     /locus_tag="IW651_002741"
     CDS             30..1331
                     /locus_tag="IW651_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0815411.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1473..1673)
                     /locus_tag="IW651_002742"
     CDS             complement(1473..1673)
                     /locus_tag="IW651_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0815412.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(1807..2415)
                     /locus_tag="IW651_002743"
     CDS             complement(1807..2415)
                     /locus_tag="IW651_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0815413.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(2500..2970)
                     /locus_tag="IW651_002744"
     CDS             complement(2500..2970)
                     /locus_tag="IW651_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0815414.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(3018..3932)
                     /locus_tag="IW651_002745"
     CDS             complement(3018..3932)
                     /locus_tag="IW651_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0815415.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            4078..4881
                     /locus_tag="IW651_002746"
     CDS             4078..4881
                     /locus_tag="IW651_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0815416.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            5059..6006
                     /locus_tag="IW651_002747"
     CDS             5059..6006
                     /locus_tag="IW651_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0815417.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         1957 a         1198 c          975 g         1921 t
ORIGIN      
        1 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
       61 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      121 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      181 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      241 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      301 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      361 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      421 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      481 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      541 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      601 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      661 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      721 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      781 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      841 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
      901 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
      961 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1021 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1081 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1141 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1201 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1261 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1321 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1381 ctatttgaca aagagccaaa aaatctaaaa acttcataaa tagacaaaaa gacacgactc
     1441 tctaagaagt cgtgtcttta ttcaacaaat tattataatt tagtcacttc agcagcttga
     1501 ggtccgcgtg caccttcaac aatcgtgaat tctacttctt gaccttcttc taggctcttg
     1561 aagccgtctc cttgaattgc tgagaaatga acaaatacgt catctccgcc gtctactgta
     1621 ataaacccaa atcctttttc attgttgaac catttaaccg ttccgtgttc cataaatgta
     1681 tagcctccaa aaaagttctt gcaagtaacc ttgcaagaac attataccca atttagaaaa
     1741 cgctagcaag ctttcgctct tttattttcc aaattatttt taagactgat atgacggagg
     1801 aaatggttat tttgctgctt cgaaacgttt gtttacttca tcccaattaa taacattcca
     1861 gaatgctgca atataatctg gtcggacatt tttatatttc aaataataag catgttccca
     1921 aacgtccaaa cctagaattg gtgtctttcc ttccatcaat ggagaatctt gatttgcagt
     1981 agaggtaata gctaattttc cattttccat tacaagccaa gcccatccag aaccaaatcg
     2041 tccagcggct gcttttttga attcttcttt aaaagaagaa aaatcaccaa aagcttcatt
     2101 aatcgcttct tttatttctc ctgtaggttc gccaccagca tttggtgcca tgatttccca
     2161 gaaaaatgaa tggttagcat gtccgccacc attgttacgt acagctgtct tgatatctgt
     2221 tggaatagcg tccatatcag ataataattc ttctattgtt ttttcgccta attctgggta
     2281 tttctcaatt gctgcattta aattcgtcac ataagtattg tgatgcttat catgatgcag
     2341 atgcatcgtt tcttcgtcaa tataaggttc caatgcgtca tatgcataag gtagatctgg
     2401 taatgtgtaa gtcataaaaa aattcctcct caatgttttc tttataatgt ctacattaca
     2461 aagagtaaca aagagaagga aattcttcaa ttgttatgcc tagtcgtctt tttcactttg
     2521 acctacttct tcttcaaaaa cctcagtttc tttaaaaata aacaacttgc tgaaaatata
     2581 gttcgctagg ataacaataa tttgagtgat gattttcgcc acatactcat tgctgttcat
     2641 aacattgatc aacaatacca ttgcccccat gtccaatccg taagaaagga tacggtagaa
     2701 gaagaactta ccgcattcaa taagtgcatg tgtaaaattc ggtgattttg aatggaatac
     2761 ccaaagtttg ttcgttacaa aagcaaacaa tacagaacaa aaccacgaaa tagaattgga
     2821 taaggccata cttaaatcaa aatattggcg tgcgagagca aaactaacaa aatttacaat
     2881 cgtagccaaa ccaccaaaaa agagatagac tagtatctcc caatatcctt ttgcttctaa
     2941 atatcctttt aactgtcgat actgcttcat gagccaacct accttattaa aaattcttat
     3001 ttatcatact ctatatttca ataactttca tctttattta aaaaaattaa ttttttttga
     3061 tcctttgcct ttgtctctgc ttggatcgtc acatgtgtaa taccatattt taattttaat
     3121 aaagaaatta tacgaggata tatcttttca atctcgctta gtagctgatc ttcaaaatca
     3181 atatgtccgt cgaaaataat tatcttttcg ctatactgcc aaatatggac atgatgaaca
     3241 ttctttaccc catcgattgc ttcaatttct tgttccattt tttcatagtc aatagcaggt
     3301 gcactttgta tcaaaatagc ggcagctttt ttgatcacca ttatcgcttc tatcaatata
     3361 tatatcgaaa tgatcagtgt aataactgga tcgatccagt agatatcgaa aaaacggatc
     3421 aaaattgctc cgataatcac acttaccgaa gacagtgtat cacttaacaa atgcaaataa
     3481 ctagatttta tattcaaact ttcatctgct tcttgtcgta ataataatac tgagaacaaa
     3541 ttcgcgagca atccaataac tgctactgtt agcatcaaat gactatttat cttttctggt
     3601 gaagtgaacc gcttgaatgc ttcaacggtt aaaaacaagg ataatatgat caatatgaat
     3661 gagttgatga aagcactcaa tatttctaaa cgctggtagc catacgtacg ttttttattt
     3721 gcttcttttc gggccatttt ttgtgccccg taagaaaaaa taattgccaa tgtatcacta
     3781 aggttatgga aagcatctga aacaagagac aaactccctg acataatccc tccaataaat
     3841 tctgataaag tgatcaccgc attcaaaacc actactccaa atagtttttt tgatgatctc
     3901 tgtggctgtg taggcactat tccttcctcc attttattta tattggatag tctttagaca
     3961 agaataacat ttttttgata tttcaccaat cattcagatg tttccctaaa gcacttgtat
     4021 ttcacttgat acttcgctag aattgcaagt gatgacacta ctagatgagg agtacttatg
     4081 acaacaaaac aattaattat cagttccttt atacgattca aagacctaaa ggagacgaga
     4141 aatgtcccat tttggaaagc cattgtctat cttttagttt tgagtataat tatggctttg
     4201 ccaatctctt ttcaagtttt tcaagtctta aacaacattc aattggatgg gcaaaagatt
     4261 gctactcgca ttcctgattt tacaatccaa aacgggcagc ttcaaaccga agaaaaagaa
     4321 ggattcattt accaaacaaa ctccattatc ttcacatttg atccagaggg aaaacgaaca
     4381 gaacaggaca tctctactga tttgatgggg aattttgtta gtgtcgggat gttaaaaaac
     4441 aaacttatca tagctcttcc taatacaggc acgacttctg ctttgttgaa caataaccaa
     4501 ttagaacttc cctacaccaa tgaatcttta aaaaacttaa caggtaagga gctccgttct
     4561 tttttaagta aagcatctat accactttgg ataaaagtcc tgacatttat attttctatt
     4621 tatcctagtt ttctgaatct gctgatcact ttgctattta ccaacattgc tgcttttctc
     4681 tatgcacgct ttcgactaac aaaagctact tttcttgatt gtttaaaaac attgatctat
     4741 tcaatttcat tgcctactat aattgcaaca atcttgatgg tctttcttcc ttcttttgac
     4801 acaagcgcct tcattgcttt tgcaggaatc tttatttttg ctcaagctgt caaaggatgg
     4861 gcaaaaatat cgatcaatta agaaataaag acaactcgtt atttctactt caatagaccc
     4921 ttttagaggt tgtgacattt ttttgtcaca gccccttttt tatttgaaaa atatttcttt
     4981 ctcaaaaaac aagcaaggcg cttcaaagtt cttcttgtat gcgctacact tagagtaaag
     5041 aaaggcggga aattaactat gtcagaaaga atcatttatg cagatcgtcc atttcgtgat
     5101 gaatttatcg aagaaatcca tactatagcg cctggttata tctttaaaac agaattacaa
     5161 ccagaggatc tacaaaatgt agagatcagt ttgggctgga actcagacta ccaagaaaaa
     5221 ctattagcct cttctacctt gaaatgggtc caatctatct cagctggtgt tgatcagcta
     5281 ccccttgaga ctttttcaaa gaaacatatt ttactttcaa atggtagtgg catccacagt
     5341 gaatcgatta cggagcatgt cttaggaatc atccttgggt attctcgtgg tctttttcaa
     5401 gcacaaaaag cacagagtga catgaaatgg ttaggttcat cggtccatta ccaatctatc
     5461 caaggaaaaa aaatactaat cattggaact ggccatattg gaaaaatgat tgcaaaaaaa
     5521 tgcaaagcat tagacatgaa ctgctatgga atcaatacat ccggtcatcc agcagaagga
     5581 atggaaaaga ctttttcttt aaatcaacta aaggaagttc ttcctgaagt agatttcgta
     5641 gtcaatatct tacctctaac ggaggaaaca actgggctct ttgatcagca tatgtttgaa
     5701 cactttgatc caaaatctgt attcatcaat gttggccgtg gtgcttctgt taagacacaa
     5761 gatctggtgc aggctttaaa taaccagcaa ctttcctttg cagcattaga cgtttttgag
     5821 gaagagcctc ttccacaaga tcatcctctt tggaaaatgg acaatgtact gattacttcc
     5881 cacattgcag gtttgacacc tgattttcaa aagaaattaa tggctatttt tttaaccaat
     5941 ctgaaaagct actttgccac ccatgagctt caaaccaatc aagtaaaatt gtctgcagga
     6001 tattaatagc tgaaaacaat attttcaaaa gaacaaagag aaggtgacaa a
//
LOCUS       DACEJR010000116         5906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEJR010000116 DACEJR010000000
VERSION     DACEJR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.122"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..666)
                     /locus_tag="IW651_002748"
     CDS             complement(94..666)
                     /locus_tag="IW651_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0815418.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(673..1335)
                     /gene="rpe"
                     /locus_tag="IW651_002749"
     CDS             complement(673..1335)
                     /gene="rpe"
                     /locus_tag="IW651_002749"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0815419.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1351..1866)
                     /locus_tag="IW651_002750"
     CDS             complement(1351..1866)
                     /locus_tag="IW651_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0815420.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1868..2152)
                     /locus_tag="IW651_002751"
     CDS             complement(1868..2152)
                     /locus_tag="IW651_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0815421.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2186..3514)
                     /locus_tag="IW651_002752"
     CDS             complement(2186..3514)
                     /locus_tag="IW651_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0815422.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3528..3983)
                     /locus_tag="IW651_002753"
     CDS             complement(3528..3983)
                     /locus_tag="IW651_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0815423.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3986..>5906)
                     /locus_tag="IW651_002754"
     CDS             complement(3986..>5906)
                     /locus_tag="IW651_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0815424.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1918 a         1147 c          836 g         2005 t
ORIGIN      
        1 attattaagt tatgtctaat caaataaaaa aaccattctc atatttgctg aataattagc
       61 atttagagaa tggtttttta tctttaaaat ctactactca aaatctgcat gtcgagcaaa
      121 catcgtttct gagctttcac ctgttttatc cattaaagat aacactaaac tatcaaaggt
      181 gagaaatgct aattgttcaa aacttgaacc cataggttgt gaaaaagtat cttcttctcg
      241 ttgattttca tctttggtaa tacctggtaa tacaagaaca gaatctgcta attgaccaat
      301 cgacgagtct tttttcatgg taactaaagc aatgcttaca ccactttgat ttgctttggc
      361 cgctaaattt tttaaactac ctgtttcacc tgagccagaa cagataatta ataaatctcc
      421 aggttgcgaa tgtggtgaac taatttcacc gacaaggctg actgaaaaac ctaaatgcat
      481 taagcgattc gtaaatgctt gcatggcaat tccagagcgt cctgcaccag ctagaaagat
      541 gtgtttagct tgaacaattt tctcactaaa ttgttctaac tctacagaat taatacgttt
      601 tgagttattt tgtaattcta agataatcgc ttctaaatag tttttatttt ggtctttatt
      661 ggtcatgatc ttttacttca cttcctctaa tgattgattt agtttatcaa tttgttcttg
      721 cggggtattt ttcccattga agatgaaacc gcctgataca aaaatatccg cccctgcttt
      781 agaacaaact ttaatcgttt gattatcaat cttgccatct acttcaatat catacgtata
      841 atcattttgt ttgcgtatta catctaactg ttcgatttta actactgtct cttgaataaa
      901 atgcttattc tctgttcctg gattgattgt cattactaat acttgatcga ctaaagataa
      961 tactggttca ataaaactga ctggtgttgc aggattaaaa gtaatcccta cttttgctcc
     1021 tgcttccttg actaactgaa tgaccttcat tgcgtgtttg gtagcttcaa tgtggaaggt
     1081 aacaatatct gctcctgctt ctacaaatct tgtgacaatt ttttctggtg tatcaatcat
     1141 caaatgaaca tcaaacacaa cgtctgttag tggacgaata tctttaataa cacttggtcc
     1201 aaaagccata tctggtacaa aacagccatc cattacatca atatgcaaat aatcaacatt
     1261 cttttctttt aagatttcaa ataactcttt aacttctgcg ataggtgcat ttaacaatga
     1321 tggagcaatt gtcattctct attcccccgt ttattctccc ataatcatga gattgattgt
     1381 tctagttcgc tctcttagtt gatcccaatc gacaaaatag acacggccca tttctcctaa
     1441 taaaagttta ccttcttgaa caattaaagt cattgagttg gttccataaa tagatgaacg
     1501 taaatgtgca tcggtattta gcatgaccca tttttctgca gggtaattgg gatcagataa
     1561 tcttgttcca aaatcaatgt gttttggccc cggactgttg tattgatttt cactcgtcat
     1621 ttttggaata atcgtttcca ttacattgtt gatgtctaca tgtaaataat catcaccaaa
     1681 aaagtttgtg tcatgcatcg tttcttcaaa aaagagtgag caagttgtat gcgggctcga
     1741 aattactaac attccattct caatacctgc tgtatcaact gtatctaaaa gttcttttcg
     1801 aatatcatga tacgaaggac gccctgaaac tgtttcaata gtcaaagtgt catgatgaaa
     1861 acgcatatta aacacctaat ctttctttta catctgcata aatggcatct gtaccaatac
     1921 ctgttaataa gcccaagcca ttcgttactt tagctttaat tacatcaggg acaattgttg
     1981 tacttactac agcatcatat tcatcaattt tatttagctc atcagcaatt ttagattgat
     2041 aaattttcac ttgatctgtt aatccttctt cttccaacca ttttgataat tttgctactg
     2101 caattgtaga agtagcatga cctgctccac aaataactaa taattttttc atattgatct
     2161 cttctttctt attttaattt tatgattaag ctttcttcac tttgttcatg tagatagaag
     2221 cacctactaa aacaactcct aatccaccta ttccaattac accaaagtaa gatgctaata
     2281 aaacaaacaa ccctactggc cataatccac ataacaacat agttactaat cctgctgtcc
     2341 ccacatcgaa acttgctaat gtaaacacat cagtgataat tggtgccaac catgttgcca
     2401 tatataatgt tgtaacaaca tacaacgaac atcctactaa tgatctcaca atatttcctt
     2461 taaaataagg aatcattaaa cagatggcga aagcaaagaa tgctaaatct ccaaaaggta
     2521 atactttatt tcctggaaga attagtgcta gcaataatga gattggtaca agcaatagag
     2581 aagttgacat aatagttgga tgacccacag ttaacgctgc atccattcca atgtttactt
     2641 ggcgaccatt taacttttta ttcgtaaatt ctttcgccgc ttctgcaatt ggcattaaac
     2701 cttccataaa cattgctacc attttaggca taatcttcat cactgccgca gtcataattc
     2761 ctaagtttcc aattcctgct acgtcatagc ctgccataat acccactaca ataccgataa
     2821 ttaggccaat aacaactgtg tcacctaaaa caccgaaacg ttttgttaat gtttctgcat
     2881 ctgcatctat tttattaaat ccaggaatac gatcaaaaac ccaatttact ggtttggcaa
     2941 atagtactcc tgataatccc atcatatgtg tgatagagat tcctggatag ccgaaaaatt
     3001 cgctaacagt tttttgtgta cgatctgcta agaaccattg taaaattact gcgacaatcg
     3061 cgccaattaa gccaaaccaa aagtttcccg taacagattg agtaatcata cctaggaaaa
     3121 ttgcgaacca aaagttccaa atatcaacat tcaatgtttt tgtccaaccc aaaaccacaa
     3181 atatgaagtt aagaattaat gaaattggaa tcattaaaat tcccaatgat gtagagtaag
     3241 ccaaaggtcc accaacacct acgccaatat ctaggatatt caggccacca ccgtattgtt
     3301 cccccatttg ttgaattgct gttcctaaac catctgataa taaacttaaa actaatccta
     3361 aaccaataga accgatacca acagtaacgc cggaaacaaa tgctttacca ggttttaatc
     3421 caaataatat cccgattaca aaaataatca gtggtaaaaa gacgtttgac cctaatccaa
     3481 taaaccattc aaaggcattt aatacaatat ccatctctct attcctccta ataaactcct
     3541 gcagaatgta ctaactcaat aaatgcttct ttcgtggtac atgcaagtaa ttcatttact
     3601 ttttcttcat tttgaaatag gctaactaag ttttgtagtg tatcaatttg ttcatgaggt
     3661 tgtgacattg ccaacataaa gactaaacgt acaggtattt catgagattt atctgtcata
     3721 tctgaaaata ccagtggttc ctttaatgag gcaaaacaga tttgagagct atttacatgt
     3781 gcggaatctg tatgtgggat agcaaaccca atttgattta cttctaatcc agttggaaat
     3841 tcttgttcac ggttggtgat accttctaag aaactatctt tcacgcaacc agcgtttttt
     3901 aattgttcag acattacttg aaacaattct ttttgatctt tcacttctaa ttgaaataaa
     3961 cataattctt tatctagata catttttatt ctcctttttg ttgaaaaatt cttagtaatt
     4021 tttcgctatc atgaacttgt aacaagcgat cgaccatttc tttttgccca ataaattggt
     4081 aaagttcttc atatactcct ctaatctcag atacatcttc ttcagctaaa ctaaccataa
     4141 tgactaattt gacttttcga tcgccccaat taatcgcatt ttttaatgct acaataaaaa
     4201 tgttggattc aatgaccatt gatggattcg catgcggtaa agcaacacca ctttctaaat
     4261 tagtattccc cattttctct ctagagaaga tagaagcacg aaactcttca gtaactaatg
     4321 aattggcttc tagttcggaa atcatcaaat ccaaacattc ttctttgctt tccacctgta
     4381 ctcctgtttt tactaaagat gattgaacaa atttagatat ggtgggaact ttaaccgctt
     4441 taggaatcag ttcattttga ataaatcgcg aaggttcttc tattaaatat ttagtataag
     4501 cttccataat attcatataa tctgttttac ttaccaatgg agataccctt actaagggta
     4561 taccacctgc atcaaaggaa agagaggaaa taatcaaatc aacttcccca atttcagcta
     4621 aatgaaactt atctaaagtt gatatttcta cattatcttt tgctggtaaa aaccgtcgaa
     4681 ctttattgta aattaattgc gcagaagaaa taccgtattg gcaaataatg atgatattat
     4741 ttgctttgga ctgatcttca atagcaatct gaaaatgaat caaaattaag gaaacttcat
     4801 cgtccgttaa agtcacatca aattcttctt caaggatgga catggcgtac catactatgc
     4861 taaagagttc tgaatattga gatttgatat tttctaataa cggattttga attttaatac
     4921 ctttttttaa acgaaggacc attgctggca catgatacaa taaggattga tataatcgat
     4981 cattttgagt caaatcaatt ttttcaatct cactcattct ttcaatcaat ttatgaacgc
     5041 tcatagcgta ctcttcattg ctggttttta aattatttgt cacacgatgt gcaaatagtt
     5101 gacgacatag atattcttta tctgcgtcat aaaatggaag attcaatttt gccttcaatt
     5161 cttcaattaa accatttgcc acaatgtacg tttccatata gcgaatgcta ttgaagagga
     5221 attcttcttc aacagttaaa tgatgacctt tcagtaaacg gttacattgg ataactaaag
     5281 tagaaatcaa tgaccgaaca tagtaatcag aaaccttttc agtcagttca ctgtaatcat
     5341 tgaatagcaa atcatgaatg acactaataa cttttgaatc aaacaacata tttaaaactt
     5401 gaaaaaatgt ttttgattcc atgtcattcg cataatagaa tacaagttgc ttgattgctc
     5461 gttgaattgc ttgttcatta ccagtaaaac taattccttc tgcagatgac tgcaacttaa
     5521 cttctccact gccaataatt ttattgataa ttctcaggtc attgtataac gatgttttac
     5581 taaccataaa actttgagac aaatgagtta aagaaacagg cttatcacca aacaaaactt
     5641 ctttaatgat ctcgacacgt cttttttctg gagaataaat atctttttca agataatttt
     5701 ctaccgatga aatcaaatgt tcaacgtttt tcctatcacc tatgagtttg atacccaaag
     5761 aaggtaatcg ctcaattgtt acttggtaat taactacata atcttccaac aattcaatat
     5821 cttgataaat ggtcttggtc gacactgcca atttatcact aaaaaaagaa gcgggtttaa
     5881 agtcttcttg tcgacttaat aatttt
//
LOCUS       DACEJR010000117         5894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEJR010000117 DACEJR010000000
VERSION     DACEJR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.123"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            142..523
                     /locus_tag="IW651_002755"
                     /pseudo
     CDS             142..523
                     /locus_tag="IW651_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            654..1604
                     /locus_tag="IW651_002756"
     CDS             654..1604
                     /locus_tag="IW651_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0815425.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2122..2577
                     /locus_tag="IW651_002757"
     CDS             2122..2577
                     /locus_tag="IW651_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0815426.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2888..3244
                     /locus_tag="IW651_002758"
                     /pseudo
     CDS             2888..3244
                     /locus_tag="IW651_002758"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3257..3667
                     /locus_tag="IW651_002759"
                     /pseudo
     CDS             3257..3667
                     /locus_tag="IW651_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3782..3946
                     /locus_tag="IW651_002760"
     CDS             3782..3946
                     /locus_tag="IW651_002760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815427.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4097..5076)
                     /locus_tag="IW651_002761"
                     /pseudo
     CDS             complement(4097..5076)
                     /locus_tag="IW651_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5081..5848)
                     /locus_tag="IW651_002762"
     CDS             complement(5081..5848)
                     /locus_tag="IW651_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0815428.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
BASE COUNT         1986 a          936 c         1034 g         1938 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgatgacact atcgttcttc atcaacgtaa
       61 gttagatttg ttgaaagagc agaaaaaagg atttctacaa aaaatgtttg tttgatattt
      121 taaatagaaa agagagataa agtgaagttt ttaaattttt tgaactataa atatttaaac
      181 tgggtattat atggtattac attatttttt attatttatt acttttttac cgatgatgat
      241 atttttttaa ttttagcaat actttttctt gtttcatcta attttagtga taacttaatt
      301 gaagttctta cagaaccaga aattcggtct atgacggatg aagaacaaca gattattaaa
      361 gctatgattt cggaaaataa gaaagacgta gtagttatca aaaagattag agagtataca
      421 aatttaggtg ttttacaagc caaaaaagta tatgaaaaaa aaaaaaaggg tctataatta
      481 caaattagat aatacattag aagctggaga caagattgtc tagataatgt gtgagataaa
      541 atttttggca ggttatcata cataagacaa aatgttttta aaactgataa cgtatttatt
      601 ccacaacaat tacgcattta tttctgagaa aaatataaag caggaggatt acaatgctga
      661 aaacttattc cttaacaaaa gatacattat tagtttcgtc agaatcaaat atcaaaaatt
      721 cttctgttgt ttatgtttat ggtgacgaac gagaatatat cgaatcttct gaacataaat
      781 atgactttaa aataggcaat atactaacgt ttgatgatcg agttgcatat gacacgatga
      841 tagataagaa cgatgaaaaa agtttgctcg taagtttttt gtttcccgag gtacatgaag
      901 gtgggcatct agataccctg acgacatctg ttgttttctt ggtatttaaa aataagttga
      961 taatttttac aaaacaaaaa ttaaaattca ttcccgaact cttatccaat atggttttac
     1021 gtgatgtcaa tcatttcgtg gaggaagtac ttttaaagat catgcaaaaa gatttctata
     1081 tgatgcttaa cgaccttcgt ggagtcaagg aaaaaattga tgatttagac tcagaaatat
     1141 caacgtccgg ttcactcaaa ccaacgtttg gtgatttatt gactcttcaa aaatacatga
     1201 tagctttgtc aacaacttat aaagctaacc ataaagcgct tgatttcatt aaaaaaaatt
     1261 tcacaactat tgacgatatt gattttagta ctaaaaaaat aattgatgat ttatacgata
     1321 caagtgatac aatggataga atcattttaa gttacagcca atatttggac aaattggaaa
     1381 atatgataaa caatatgatt tcgtatcagc taaacatgat tatgaaaacc ctaaccgaga
     1441 tatcaattgt tttgactatt ccagcaatta tttttggctt ttggggaatc aatgtccatg
     1501 tcccgtttga aaagtcttct tatggtgttt tagccgtatt gattatctct gctatactaa
     1561 gtctaatttg ctggcttttg atgcgacgca aaacatattt gtaaagtatt tgtgataccc
     1621 taatatgatt taaagcagaa tttctttgtt gactttttaa aaggctgatt cctttcaata
     1681 atttaatagc ttcagcggct gctcagaagt caataaattg gactattttt attcagttac
     1741 ttaggcaaca tttgagatac gattgcgatt ataaaaattg atataccaac caatggctgc
     1801 ttggaagact gacatattag cctcgatatc atcgaataac taaatacaga aaaatacaat
     1861 aaatattatc aaactaggga ctatactaga aaacatcagt acattcctta actcagggtc
     1921 tataattaga aaatcgcccc tttgaaaact gattaaaaca gcccccatta tctagtgact
     1981 agataatggg ggctgttttt ttgttaagtg atataataaa ccataaaaga agtttagaaa
     2041 tacggtcctg gtcatcaata aataaacaat ttaattaatt atttatttaa ttatttaatt
     2101 tcagaaaggt atacatgaat catggtgcaa caaattgtcc tacccatcaa agactccagc
     2161 gtcttaaaga tggtacaaga tacacttctc gatagtttcc gtgctggtcg ccgaaattac
     2221 accgtttttc aagttggtaa ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa
     2281 aaatcagatg tctataatcc agacggctct gtcaaaaatg caacctttat tcatgataaa
     2341 aagaccggta aagcaaacac gttatattta aagcctgttc agcaagactt gttgcaatat
     2401 catgattggc tggcccaaga aaatatcaat tctgagtggt tatttccctc gacagcccat
     2461 tatgatcttc atatcactga gaagcagttt cataaggtga tggcgcatgt tggtgatctc
     2521 ttaggaatca acttggaaga caaagagaaa aaattgcgag tcagaaaacc gttttgagat
     2581 atgaacagca tttgccttca cgccttagcg attttaaata atagccttga aagtgattat
     2641 ggaaaagccc tctacgctca atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa
     2701 ggtgtatttg gtatgtgaag aacccatgtc agaggcaaga acgttgcacc tactgacatt
     2761 ggaatgatgc ttatgagcat gaatctaacc aaattatccc ttgaggctag aagaaaagcg
     2821 gaagcttttc aacacaaagc agccaaaaat aaaaaacgcg acgaagcaat cacatttatg
     2881 attattcatc gcgtttttta tatttagagc tagttatttg ctagacccac tatttacaga
     2941 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     3001 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     3061 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     3121 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     3181 gattatccat aatgcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     3241 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     3301 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     3361 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     3421 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3481 ttttaaaaaa gaaccttttt tcgaggtgga agctaagtaa aacaagggtc atttaagggc
     3541 gttctaagcc ccacacagcg ttttaaaacc caagaggagc tttttagctt ttgtttcttc
     3601 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     3661 gaaaacgaat agtccgagtt cgttttcttt aagtagtttg caatcaaaag tcaatcagct
     3721 ttataaagcg acagcgagcc aaactttaaa agctatgcaa ggactgtatg aagcaaaatt
     3781 attgagttat ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa
     3841 agcgaatttt ggcaaatata gcggtttttt aggacttgat cttgaaatgt tgttatcatt
     3901 gataatgata cgttcaaaca acagcaccca aattttgacg aattagtgaa actctatgaa
     3961 aaagacgtag taaaacatgc aacatcctat tctaatcagc tggtaaagct taattaggtt
     4021 atataagctt gaaaataaag ggtttgtcca atatggacaa gcctttttct tatgttttgc
     4081 cacagatttg ccacacttat tgtgtaagag tatcaaggat aggtataatt tgatactttg
     4141 tttttttagt gatgtgcaaa taaatttctg ttacacgact acctctagaa tgccctacac
     4201 ggtcttgaat tgcttctagg ggtattcctt actctgctaa gaaactaata tgcgtatgac
     4261 ggaatatatg ggtcgtaata tgtttattaa aactaagttt attatcaata tcaattcctg
     4321 ctcgttttgg attacctttt cgatttaatt ttccattttc aggtgagttg aggaaattgt
     4381 taaggacact ataatgaaga ggtgttccat tatagtcggg tttatgacga cttttggaag
     4441 taaaaatcca tccattatct ttataatcag gattgaatct catatgttgg tgatttaact
     4501 caatttcctc tttaagaatt tctatagccc ttttagataa ttggacattt cggtaacttt
     4561 gtaagttttt tacattacct tcatagctat tttctgcagc tcctccgtga gtggactgac
     4621 taatgatgat attttgcttt tcaaaatatt gataggcacc aatattttgg agtttcgact
     4681 ctgtaagtca acagggccat tagttagaat acctattggg atgcggtttt tatgtagagc
     4741 cagcagtagt tgatgtatct caggaatcat cgtaatctga tcgagttcat attcatagtc
     4801 agcttggaag tctagagctt ctgtcctaga aatactatcg atgccaaatt cttccatagt
     4861 cttctgacag cgaaaaattc taagctgctc aatactgatg agttttttcg tgtatttagg
     4921 aaaagcaaca tcagaccaat atctgaaacg tttgtaaatg ttatctattt gttggatatc
     4981 aatcgttgga aaacatttct caacgctacg tttaaagggg agttgatggt cataaagagt
     5041 gtcatcaagg tcaaaaacaa tatttttata agtcataatt ctagtccgct tttaaaatag
     5101 tagtgtactg gctaaattcc tctaagtgtt gagggctaat ctggttatct gtaatgatgg
     5161 tatcgagttg atctagatta tagaagttaa agaaatcgta tcgatcgaat ttagtactgt
     5221 ctactaataa gaatttttct acagcattgt ttagggcaag ttgttgaatc acaccttcct
     5281 tgtcactata tgtagcaata gaattatggg taacagcatt tgcactaaca aaagctttgg
     5341 caaatctaat tgcttttaaa tttgtcgaag ccattgaacc tacaaaagct ccagttattt
     5401 ctctatattc accgccaaga agcaataaat caatggtttc gctatcattt agaatcaaga
     5461 acacagggag actatttgta atgacacgaa ttttataacc tttacgacct ttcaattcta
     5521 atgccagttg ttcaagtgta gttccaggtc caataaaaat agtatcgcca tctttgatta
     5581 aagagcaggc ttttttagca atgtcaattt tttcttttgt atttagactt ttcttctcga
     5641 tatgtgtctt ttcaagtggc tttttagagg aaagcaattg tgcaccacca tgagtacgtg
     5701 ttaaaagtcc atcagcttct agagcatcaa ggtctctacg ggctgtcata tcggaaatat
     5761 ctagttcatc tattatttct tttatggtta tggtcccatc aatctttaac atatctaaaa
     5821 ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt ctttcataaa gtaatttttt
     5881 tccaaagata attc
//
LOCUS       DACEJR010000118         5665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEJR010000118 DACEJR010000000
VERSION     DACEJR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.125"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..194)
                     /locus_tag="IW651_002763"
     CDS             complement(<1..194)
                     /locus_tag="IW651_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815429.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="IW651_002764"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="IW651_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="IW651_002765"
     CDS             3033..3608
                     /locus_tag="IW651_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815430.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR"
                     /locus_tag="IW651_002766"
     CDS             3822..4517
                     /gene="vanR"
                     /locus_tag="IW651_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0815431.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="IW651_002767"
     CDS             4495..5649
                     /locus_tag="IW651_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0815432.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1730 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aattt
//
LOCUS       DACEJR010000119         5661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEJR010000119 DACEJR010000000
VERSION     DACEJR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.126"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IW651_002768"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IW651_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0815433.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="IW651_002769"
     CDS             complement(3252..3770)
                     /locus_tag="IW651_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0815434.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4888..5661
                     /locus_tag="IW651_002770"
                     /pseudo
     CDS             4888..5661
                     /locus_tag="IW651_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1914 a          928 c          894 g         1925 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccacttag cttggatagg ctacaattaa ctagacagaa aaattaaggt gtgtagacta
     4861 gaagaaaaca taccaggagg aatttttatg tctaagagaa cacgaagaac tttttcacaa
     4921 gaattcaagc aacaaatcgt caatctttac ttagctggaa agccacgtgt agaaatcatt
     4981 cgagaatatg aactaacggc ttcagcattt gacaaatggg taaagcaatc taaaacgagt
     5041 ggttcattca aagaaaaaga taatcttacg cctgaacaaa aagaattgtt agaactacgt
     5101 aaaagaaacc agcaattaga aatggaaaat gatattttaa agcaagcagc gctgatattc
     5161 ggacgaagag acaagtaatc gatgcgaata agcatcttta ccctatatca gcgatgtgca
     5221 gaatattagg tctatcacgt cagtcctatt attatcaatc aaaaccaaag aaagacgaat
     5281 cagaacttga agaagtagtc gctgaagaat ttatccgcag ccgaaaggcc tacggctcaa
     5341 gaaaaataaa aaaagcctta tcaaaacgag gcattcagat cagccgacga aaaattagta
     5401 gaatcatgaa aaatagagga ttaaaatcga gctatactgt tgcttatttt aaagtacatc
     5461 attctacttg caatgaagcc aaaacgacaa acgtattgaa tcgtaaattc ttaagagaca
     5521 acccattaga agcgatcgta acagacttga cttatgtacg agtcgggaaa aaatggaatt
     5581 atgtctgttt cattttggat ctgttcaatc gagaaattct cggctattct tgtggagaac
     5641 ataaagatgc cgttctagta a
//
LOCUS       DACEJR010000120         5624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEJR010000120 DACEJR010000000
VERSION     DACEJR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.127"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1350
                     /locus_tag="IW651_002771"
     CDS             <1..1350
                     /locus_tag="IW651_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0815435.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="IW651_002772"
     CDS             1484..2827
                     /locus_tag="IW651_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0815436.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="IW651_002773"
     CDS             2939..4390
                     /locus_tag="IW651_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0815437.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="IW651_002774"
     CDS             4739..5371
                     /locus_tag="IW651_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0815438.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         2168 a          783 c         1081 g         1592 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg ggaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagc
//
LOCUS       DACEJR010000121         5298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEJR010000121 DACEJR010000000
VERSION     DACEJR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.128"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1106
                     /locus_tag="IW651_002775"
     CDS             132..1106
                     /locus_tag="IW651_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0815439.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IW651_002776"
     CDS             1088..1549
                     /locus_tag="IW651_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0815440.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IW651_002777"
     CDS             1599..2063
                     /locus_tag="IW651_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0815441.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IW651_002778"
     CDS             2077..2826
                     /locus_tag="IW651_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0815442.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IW651_002779"
     CDS             2826..3656
                     /locus_tag="IW651_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0815443.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IW651_002780"
     CDS             3656..4303
                     /locus_tag="IW651_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0815444.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IW651_002781"
     CDS             complement(4401..5177)
                     /locus_tag="IW651_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0815445.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1817 a          717 c          949 g         1815 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaa
//
LOCUS       DACEJR010000122         5141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEJR010000122 DACEJR010000000
VERSION     DACEJR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.129"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..1156
                     /locus_tag="IW651_002782"
     CDS             116..1156
                     /locus_tag="IW651_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0815446.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            1980..2312
                     /locus_tag="IW651_002783"
     CDS             1980..2312
                     /locus_tag="IW651_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0815447.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2931..3272
                     /locus_tag="IW651_002784"
     CDS             2931..3272
                     /locus_tag="IW651_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0815448.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(3509..4759)
                     /locus_tag="IW651_002785"
     CDS             complement(3509..4759)
                     /locus_tag="IW651_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0815449.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1792 a          768 c          980 g         1601 t
ORIGIN      
        1 gtatttaatt aaaaaacagt tttaatgcgt ttgcattttt tatttatctt tgctattttc
       61 gaaaagagta ggtaatttta cataaataaa acaacgtgaa agtagaggat ctacaatgac
      121 tgattttaaa ttttttaagg cggatagagt ttacaacgaa ttattttatc aatttccaaa
      181 agtctttatt gtttctgacg aatacaaaaa aatgaaagat tcaactaaga ttgcctatat
      241 gcttttaaaa gcaagattag agatcgcaat cagcaaacgg caaatcgatg aagaaggtaa
      301 tgtttatttt acttatacga caaatgaact atgtagagta ttaaactgcc aaaaacaaaa
      361 agcgatagca atcaaaaaag agttggaatc ctttggttta ttattacaaa agcagatggg
      421 atttaacaaa cagttaggga aaaataatcc taatagacta tatctagcag aattaaaagt
      481 ctcagaaaat gatatctact tactcgaaaa atttgataga gagaataggg aaaacgttga
      541 taaatcagag ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg ctgaatccct
      601 tggggctcaa gagggtatga aaatcatacc gtgccaaaac gttgataaat cagagggtat
      661 gaaaatcata ccagaactta ataataatat attagacact aatagacaca atatagacac
      721 tgaaaaagac cgcctacaag atcaattgtt gttagacaat tttgagacaa ttatgacaaa
      781 cgacagcatt gctacgtttg tccctgaacg agtattaaat ttgataaaaa cattttcttc
      841 aagttacagt gaagctcaaa aaaccgtcca gactattcat aatgcaaaga aaaaagctga
      901 aatagaaagt ggtatttcga tagtttttga agaactcgat agttattatg tcaatgcaga
      961 acaagaatta tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg aaaaagtcga
     1021 aaatatccag aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa aaccaatagc
     1081 tgctaaagta tcaagtgaaa aacgtttgaa ttatgaaagc tccccaagca ctattacgaa
     1141 agactggtta gagtgaaact ttactgacaa agtgattgtt gcttcatgga gtaacattaa
     1201 aattaaaaat tttttattat tctaattata ttacgaatat tacaaaataa tagctatttt
     1261 aatagaattt ataagttaaa atattatgat aaaaatcaca aacgtagcct tttacgtcat
     1321 aagtgttaaa agaaataatt aatttcaaat atagaaaagg gaacgtatgt ttgcatactc
     1381 cttttttttg agttataata atacgcgaag agagataatt tcattattta aacaagctaa
     1441 aaatcttgat attacttctt ttcaggcaat acgaatatta ttcgcttaca ttttttgtat
     1501 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggatgat
     1561 aaatttggac aattgaaaat acaaaaaaaa gaaaggcggt atgaactcgg aaggctggta
     1621 accttcgtca gcgttcatac acggcattaa gatttggccc ttaatgcgtt aacctttcag
     1681 tgttacggta ttacccgcaa tggatttagt ataacagata ttcaaaagtg ataccacttt
     1741 ttttggctgt gttaaggtta aaaatacgtg atattacgat gaatctatgc ctaagcacaa
     1801 tgttataaaa atgtccgtgt ggctctttgc cgtatgacga acgctgaaat tagagggaac
     1861 gcatgtttgg atgcgttccc tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt
     1921 ttgaaaaata acaagaaaaa cttgttaaaa atatttaaag aaattgggtg gtttatttta
     1981 tgtcatatac aggaattttg agtctcaaag atatttgtca ctacggtaaa cggtgtaccg
     2041 ctacagagaa aatcacgaag aaactatcaa ctggccaaag taaaaccgtt gtgcagtgta
     2101 agaaatatat aatccagaaa gacaaagtct cagaggaaat gatttattac attgggaaac
     2161 aaaagcaaat aattttaaaa gatcctatcc ccttaaaaga gctatatcca acaatcaaac
     2221 atgtttatga ccaaaacggt gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta
     2281 ccgcaaaagg gatggggcgt ttgatcggct gaattgatca ttctcataac ttgtatttta
     2341 tcgtttttcg cttgcttgaa aatattgtcg agacttctaa aggaacttag agtcactaga
     2401 aaaataagga gtagacttga tctatcaaca attcccttcg gggagtgacg gggtaggttt
     2461 taacgattgg taacgtaaaa aattggtcaa tcgagtaaat gcgcttgttt ggatagtggt
     2521 gaaatgaatt tgtgtaaata ataagcgcag agtaaacaag agggaattta gaagttagac
     2581 aagttagaat agcgtctaga ttaatcatga gcagctacta agggatctag actttgacgc
     2641 aaactcatgg aataaggagt aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt
     2701 cgtgtgctgc agtacatgta gatacttgcg aggacgttga ttattggatc aatcgagcat
     2761 ttaccgctgt ctactagggt ttctcgtttc ggacgccata aacgagtcga aattttttat
     2821 gtatttttaa gtaaacgaaa aacgataaaa tatgaacaag tcctgaataa agtgaaaaaa
     2881 ttgatataaa acatagagaa aatgaaattt acgtaatgga ggaatgacaa atgaaaaatt
     2941 atgatccgaa tatacgattg ggcacacata ctattaaggt ttctttccaa cgttgggatt
     3001 acaaaggatt tgtgacgttt agaagaggcg gaaattgtaa aggattagat gttttagcat
     3061 tagacgagga tgatttatac gatcaaacat tgactgataa tccaattgga ttcggcttgc
     3121 tacctgaaga tgatgaaggt aatgaatggt ttaaaatgac gcttatgaat gataatggag
     3181 acgaattatc tgttgaagat acttggtcat atttgagtga ttatattgtc agctttgaaa
     3241 tcattgaatt tgtagcggat aaagaagaat aaaaactgga ggaataaatt ttacagataa
     3301 aggcttagaa ggagtcacga tgattaataa aaaggtagta ttaccttagg cattgagtaa
     3361 ggaatttatt tagtctaatg gtgataagtc aagatgaaat ttaagatttt ctttattgag
     3421 taatatgttt tgaagcgcac gaaactagac ccgatcaaaa tcaaattttt ttcgggttga
     3481 ttcaaggatt tgcgtcaata acggattgtt atttctctat cacactcctt ggtggcgtat
     3541 agagttggtg tttacgtagt agcgtatcca ccagacgcgt aaattttctt gcggttagga
     3601 cgagtgcacg tttgtgttga tgctttggaa cctccttata tttactttga taaaaagctt
     3661 tgtattcagg aagataattt tttacagagt tggcagcttc aactaagtag tatctaaaat
     3721 atcgatttcc ttgttttgtc agaggtgtat tttgcgactg atagtttcca gattggttca
     3781 ctttccaact taatccagcg tattttgcca attttgtctg atcttcaaat ctttcgattt
     3841 gaccaatttc agcgattaaa ccggcagcat agactttacc aacgcctgga atactggtta
     3901 agcattgata ttctggaatg acaacaatta cttgttcgat ggctttatct aattctttga
     3961 tatttttttc aagtgctcgt atttctcgta cgagaacact taaaacaaca ttaattgatt
     4021 cttgtgcaag agcacctagg cggtaactca tggtaattgc acgttgaatt gctttagcga
     4081 ttttttctgg ttgcttgaaa cggccttttc ctttttcttg aagtaaatca cagaaagctt
     4141 ctaaaggaag ctcagcaagt tcatccaatg tgaaatcttc cgtcatgagt gaaatgaggg
     4201 ttgcactaaa aagactcgtc gattcgtctc gcatttctcg agcaagcgta ttacatttgt
     4261 acgttagatt ttcaagaaaa tgttgctttg tacgaatcaa ttgcttaata agttgatagc
     4321 gagtacgtgt taaacgctgt aaggccatgt atttctcttc tttgataggg gaagttgtga
     4381 atcgttgaat acgcaagaaa tcagcgattc ttaatgcatc gtttcgatca gttttattct
     4441 catcaaacat gcgactaaat tgtttcaccc gaaaaggatt ttcaatcgtt acaagtgtgt
     4501 tgagcttctt tagtttctca tcttcatgaa aaaacatgga aggatggaag ctgtacatgg
     4561 acgttgactc catgccaatc acaatttgat caaagtggta gctgtcattg aattcaagaa
     4621 ttgtttctcg tatgaatgaa gcaccttcga tgtcattggc aacagagatt tcagataaga
     4681 tagacaattg attgtcgtct gttaaaaaac aaacatctag tttttcagag ctaacgtcaa
     4741 taccgacaaa taatttcatg gaagaaggac ctcctttcga tataaatttt ggaaaatgct
     4801 tcgatactgt gggatttccc agaaactcac tgtatagcca caacctcgcc taatagaata
     4861 cggtgataaa agagctagaa gctgccttcc aaactactat ttgaaagtct agtccattat
     4921 cgaaacagct aatgagttgg tagtgataac agtggttcgg gtaacagact aaacagggta
     4981 gacaaacgct acaggaggaa atagcaaatt cccgttggat cctttccata catccattgt
     5041 actctgagaa atcacacaat atcgaaacaa attccgttag gaatgtgtta ttcaataaat
     5101 tttataatct agaaacattt tgaataaaaa ctattagaca a
//
LOCUS       DACEJR010000123         4998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEJR010000123 DACEJR010000000
VERSION     DACEJR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.130"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(358..1110)
                     /gene="xth"
                     /locus_tag="IW651_002786"
     CDS             complement(358..1110)
                     /gene="xth"
                     /locus_tag="IW651_002786"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0815450.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1223..2143
                     /locus_tag="IW651_002787"
     CDS             1223..2143
                     /locus_tag="IW651_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0815451.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRISDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2216..3151
                     /gene="murB"
                     /locus_tag="IW651_002788"
     CDS             2216..3151
                     /gene="murB"
                     /locus_tag="IW651_002788"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0815452.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3449..4927
                     /locus_tag="IW651_002789"
     CDS             3449..4927
                     /locus_tag="IW651_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0815453.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSLISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1746 a          805 c          962 g         1485 t
ORIGIN      
        1 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gaggaactat
       61 ttttgtttgg ctgttcttat tacttgatgc agtgcagctt tttcacaacc tcttaataaa
      121 cggtgttcaa aagcttgtat ctgcggtaat aagcccaacc aaacgaaaaa tatgaggaac
      181 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca atctctcgat
      241 aaacagtgtt caaaagccag tttctcggta ttatgccaaa tttatgcaaa atgtggaaag
      301 ccattttcac aaaatttttc ttaatactcg aaactaaacg actttttcac aacctcttta
      361 tttcaaatct aattctactg gacaatgatc actgcccata atgtccgtat ggatctttgc
      421 atcagctagt cctggctcta aatctttgct cgtcaaaaag taatcgatcc gccaaccagc
      481 attgtttttc cttgaattga atcggtagtt ccaccaagag tagacacctt cctgggttgg
      541 ataaaaataa cggaatgtat cgataaaccc gttatccaac agtcgagaaa gagccgctct
      601 ttcttctgga gtgaatccag catttttttg attcgttttc cagtttttca aatcaatctt
      661 ttgatgagcg acatttagat ccccacacac gatgactggt ttttgttttt ttagattttc
      721 caaataatta taaaaggctt cttcccattc taatcggtaa tctaaacgtt ttaattcgct
      781 ttgagaattt ggcgtgtaac aggttacaag aaagaaatcg gaatattcca aagtaatcag
      841 acggccttct gtatcatgga tatcgatacc cattccgtaa gtggcattca atgctggttt
      901 tttagcaaaa atcgctgtgc cggaatagcc ttttcgttcc gcgtaattcc aatattgata
      961 ataacctgga agatctaaat caatttgtcc tgcttggagt ttcgtttcct gcagacagaa
     1021 aaaatcggcg tctaactcat gaaaaacttc taaaaaattt ttatttacga tggcacgaag
     1081 cccgtttacg ttccaagaga taaatttcaa taaaagcaca ctccttcgaa ttaatttcct
     1141 tacctttgcg atactattgt acctcatttt agtaaaaaat ggtgtaagat gaaagtaact
     1201 aaatgatcca ggaggaaagt agatgaagat tggtgtaatc ggtgtaggga atatcgcaga
     1261 aaaagcatat ttaccaactt atgcgaaaaa acaaggaaca cttgattttt attttgcaac
     1321 aagaaataaa gaaacaaaag aaagaattca aaaagagtac gggttccaac atctatatga
     1381 aacgatcgat gagcttttag aagaaaaaat cgaagcatgt atgatccacg cagcaacaaa
     1441 agtacattat gaattagcca aaaaatgtct agaaaatggt atccatgttt atattgacaa
     1501 accactgagt gtccatctaa atgaagttcg tgaattgcaa gaattagctg ataagaacca
     1561 aataattttg atgatcggtt tcaaccggcg ctttgctcct atggtagaag aattgaaacg
     1621 tatctcagat aagcggttga tccacctgca aaaaaatcga atagcggcaa aagaaacgac
     1681 agagttcgtc ctttatgatt tgtttttaca tcttattgat acagctgtgt atttattaga
     1741 tgaacccatt ttgagaacaa aataccagat aagagaaaca aaggaatttt tggaatatgc
     1801 cattttacaa ttagaaacac aaaatcaaac ggctatttta acgatggatt tagcaagtgg
     1861 cgctaatata gaaaaatatc aagtaacaag taaaaatggt acttatgaag taaacgatct
     1921 gacccagctt gtcattcaag atgctgatgg aaaaaaagtg aaagaatttg gcgactggac
     1981 aaacacgcta gtcaaacgcg ggtttgagcc aatggtagag gccttctttt ctcagatccg
     2041 tttaggtaaa tcggaaacag aattgcttaa acaaaaagaa gttgtaagaa gtcatgaatt
     2101 atgtgaggaa gtgcttaaag atcactaccg tcatcaactt tgaacaagac tggcaagatt
     2161 gtattaagcg tgatagaata atgaggacgt ttgagaaaag aaaggaaaat cctcagtgaa
     2221 taaagaagaa atagttgaaa gcttagccac gattactata ttgtttgatg aaccattgat
     2281 gaactatacg tttacgaaaa caggtggacc agctgatgtc cttgcattcc cgaaaaaaca
     2341 agaagaagta aagcaaatca tcgattattg ccgaatccat gatatacctt ggatggtatt
     2401 aggaaatgca agcaatctga tcgttcaaga tggcgggatt cgcggtgtag tcatcatgct
     2461 gactgaaatg aaacagattc atgtcaaagg aacgatggtc attgcggaag caggtgcctc
     2521 actgattgat acgacttatg cagctttggc agaatcattg actggatttg aatttgcttg
     2581 cggcatcccg ggaagtgtcg gtggagcggt ttatatgaat gcaggtgcgt atggcggaga
     2641 gatcaaagat gtttttgctg aagtggatct tttgcttgaa gatggaacat tgaaaacttt
     2701 gacaaaagac gaaatggtat tttcttatcg tcacagtaaa gtccaagaat tacgtgctat
     2761 cgtattggaa gctcgattca gtttgcaaac aggggattat gaagcaatca aagcaagaat
     2821 ggatgagttg accgaattgc gtcaatcaaa acagccatta gaataccctt catgcggaag
     2881 tgtctttaag cgcccagtag gacactatac aggtcagctg atccaacaag caggattgca
     2941 aggcctgaaa tggggcggtg cacaagtctc tgaaaaacat gctggattca tcgtcaatat
     3001 cgatcatgcg acagcgacag attatattga attgattgcg cacatccaag aagtaatcaa
     3061 agaaaaattt gatgtgtctt tagaaacaga agtgcggatc attggagaaa aacaagcaat
     3121 atcagaaaca agcagaagtt tagccaatta ataagtaatg gaacttaaaa taagtaaaag
     3181 agcaggacgg tggccgtcct gctctttttt gtggttgttt acgcaaatgg cgcaaatctt
     3241 gtacatttta acacaataaa acaaaaaata tagaatattt cctcataaat ttttccattt
     3301 tttgcaatat tctcaaaaaa aagataaaaa atgagaataa ctgatgaaaa aacaagcata
     3361 atccatcata aaatcagtat aggtgaaaag atgaactttc atctgcatat caagtaattg
     3421 gtatgaggca ttttttgagg tgtaggatat gaagttggat taccttatag aagaggaaga
     3481 tagagaaaaa ctgctgctag ctagatattt agaatttgag aaaaagaatt atgtagtgct
     3541 gcctaagtta tatgaaacat taggtttgtc taaatttaaa acaagaaatt atatcataga
     3601 attaaataaa gaatttactg aattcaaaac aaagccagag atccaaattt cagaagaagc
     3661 agagatccat gtcgaacgtc tgaatttgac ggatatcaaa aatttaagaa gaatctactt
     3721 tcaacgatca aaaattgctc gactgttaac ggagatcgtg gattcaaaag taacgatcga
     3781 aaaatttgca gagaatcatt ttttaagcat atcaagagcc tatgtaaaaa gaagagaatt
     3841 agtctctttt cttgaacagc atcaaataaa gttaaagaaa aacatattag ttggggaaga
     3901 aataaacata cggaacgtat tatttggggt atatttcggg gtctttaatg gtttttctat
     3961 gccatttaga gaaagcgtca tgaatgaagc aaagagtatc gtgcaatatt ttgaatatct
     4021 gttcaattta aaactgtctg caacaaaacg aatcaaattc aatttgctaa ccactattag
     4081 tttgttgagg gcctatggga atcaaccaat cggaaaacgt ttcttttcag atgaatactt
     4141 taagagctca gaaggaatga agatcatcaa ggatttatca cttatttctt ttcttcatat
     4201 ccctaaagag cagatcattg atgagatttc ttatatcatg ttgtttttga tcacggaaga
     4261 agaatttgaa ttagatggcg catttggact tgcggaagct cattttcatg atatccacat
     4321 gattagtcaa aaaatgtcag tggaattgat tgatcaatta ccattttcga ttgaaatctc
     4381 tgaagaagaa agactatata tacgaaaaga acttcgtatg ggtatgatgc atgtcaatcg
     4441 aaaaaattgg atattcgact ttgagatgac tagctttacg tctaaaaagc agctacagtt
     4501 tttcttcgag acgtatccaa tattcagtac agcaatccaa aaggtcatac aagagaataa
     4561 acgctatttc ttgggaaaag atgaacgttt catgattcgc ttattttatg attatctctt
     4621 tttattggtc ggtatttgtc cggtatcata ttttgaagca ccgattcatg tctgtattga
     4681 tttttcgcaa ggatctgctt ataccgatta cattatttct caagtgaaag gatttaaaaa
     4741 ttacaatatt gtgattgaga gtcgaatcac ttctcgtacg aatatttatt tatctgattg
     4801 tttacttgaa aaatttaata aagaacagat aatttggaaa aatccaccga ctccagatga
     4861 ctgggaatat tttggaaata ccatcatcag agaaaaaaat agacatttaa ctgaaaaaat
     4921 cagctagtat ttatggatgg cagctttgtc tcacatattt attagctgat ttatgttttt
     4981 ttcaaagaaa aggtaact
//
LOCUS       DACEJR010000124         4829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEJR010000124 DACEJR010000000
VERSION     DACEJR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.131"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW651_002790"
     CDS             complement(207..1169)
                     /locus_tag="IW651_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0815454.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW651_002791"
     CDS             complement(1171..2610)
                     /locus_tag="IW651_002791"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0815455.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW651_002792"
     CDS             2795..4741
                     /locus_tag="IW651_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0815456.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1545 a          823 c          791 g         1670 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaa
//
LOCUS       DACEJR010000125         4818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEJR010000125 DACEJR010000000
VERSION     DACEJR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.132"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..270)
                     /locus_tag="IW651_002793"
     CDS             complement(<1..270)
                     /locus_tag="IW651_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815457.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFC"
     gene            624..1472
                     /locus_tag="IW651_002794"
     CDS             624..1472
                     /locus_tag="IW651_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0815458.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1617..2558
                     /locus_tag="IW651_002795"
     CDS             1617..2558
                     /locus_tag="IW651_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0815459.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2548..4719
                     /locus_tag="IW651_002796"
                     /pseudo
     CDS             2548..4719
                     /locus_tag="IW651_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1510 a          942 c          875 g         1491 t
ORIGIN      
        1 acaaaacggg ggtgattgga tcagaaaaac aaacaagaga taaggttcgc tatatccttg
       61 atcataaact aactgaacgg ttttcttatg aagaatcagc tgaatgggac agtcgctttc
      121 cgccaaacgt ttcagatgcc gatccactgt tttaacatcg attttcagtt cttcagctaa
      181 atcagctttt cgccagatca aatctttcat tagattgatt agtgaaaata aattctgtgc
      241 attttttgat aatagttttt gccatctcat cgatcatttc tcacctgatt tctaaacata
      301 ttgtttgatc aaaatgggtt cactcctaaa aagataaaaa actccctttc tatttattta
      361 aatacgcaaa tcttcctcat tattttttat tttactcaat ttaatcgtat tctcatttca
      421 aaacgaatgt actttaaaaa aacataataa gatcactttg cttgtaggat gtattccctt
      481 ccattacaat gaaataaata gcgattttat gagaaggtga tgtgaaaatg gaagaaaaaa
      541 tagaagaatc caaacaaaaa atcgatcagt tgatataaga attagaaaaa gcggtcatcg
      601 gcaaacgttc aactatccaa ttgatgatcg tggccctatt agcaggtggt catgtcttat
      661 tcgaagatat tcccggtgta ggtaaaacgt tactggtcaa agcactagca aaagctgtac
      721 agggaacctt cagcagagtc caatgtactc ctgatctgct gcctagtgat attcttggtt
      781 tttccgtata caattcacaa tcaaaagagt ttgaatttcg tccgggaccg atctttacca
      841 cgatcttgtt agcagatgag atcaatcgta cgactcctcg tactcagtca gcacttttag
      901 aagcaatggc cgagaatcat gcaacgatcg acaacaatac ataccctcta gacaatcatt
      961 tttttgtctt agctacgcaa aatccaatcg agtacgaagg aacatatcct ttgccggaag
     1021 cacagctcga tcgtttttta ttccgactac aaatcggtta tccttctttt gatgacgaac
     1081 ttttgctact tttggataaa ttcgaaaaag tgttggataa cttaaatgaa gtattgaaca
     1141 gttatgaaat agaagaaata aaaacaaatg tggcgaatgt ctttgtcagt cccgaagtgg
     1201 catcttatgc cttgcagtta gtgtcagcaa caagaagtca tgctgccatc cagcttggca
     1261 tcagtccacg aggaagcctt tcctttattc aggcagcaaa agcttatgct ttaatccatg
     1321 ggagaaatta cgtcacgccc aaagatttgc aggatttagt tccttacgtg ttttcccatc
     1381 gcctgatttt ttacgatcgt tcattgaagg aagacgaaaa gaaacaaatc attcatactc
     1441 ttgttgcaca aattcctatt cctgtgaggt agattgaggt agacaatgga aaaaatcaaa
     1501 gattatttca ttaattttgt gttatttctg gtatatttca tcattgtgct ttttgctctt
     1561 gtattcaaca attctttagg ctggttcttg ttggcctttt ttactcttta tgtatcatgg
     1621 agcttgattt cattaagcag ctttctaaag aaactttcgg ttacttgtac attgcagcca
     1681 ctcgtttatc gcaaaaaaag tatgcaggtt attttttcca tagcaaaaaa gagaactttt
     1741 tctttacctc tccctcgttt acaaatcggc ttcccagcaa tctttgcaga aaaaaacaag
     1801 agattttgat tcttacaaat aaaccgcaag aagtccaatt gctttggcag ccaaaagaaa
     1861 gaggatgcca tgaaacatta actgtcattt ttacagccta tgatagtcta ggacttttca
     1921 gaaaaagtgc aaaaagagag atctcttttc ctgtgactgt tctgcctgct ttttgtaaga
     1981 ttcaagcaga aaaattacag agagttctgg aaaaaaaaca gcagctgaac gcgtaccaaa
     2041 gaggtcttga ttttcgggaa catcgcgctt atcgtccagg tgattctttc aaccgaatcg
     2101 attggaaatt gtctgctcat actcaagagt ggttataccg tgaatacgat tatcaagaag
     2161 aagaatgttc tcctcttttt tgcttttggg gttcgcccag tccaaaattt gaacctctgc
     2221 tagacttgta tttttcttta tggcatttga gaaaagaaaa gcagccggaa cttctgatat
     2281 taggcaaccg cgcctttcat ggcaaagacc ccggtcatac ctattttgct tctttggaag
     2341 ctggcgatga gaaaactttt ttcgcacact tgaaaaaata tgcaaaaaaa caaatcgtcc
     2401 tctttgttcc tgagaattct cctgaagtct cagaagctat tgaaacattg tcaaaagatc
     2461 gaacggttta tcttgtttat ttcgtagaaa aacaactcat ggtccaagaa aaagacaaga
     2521 tatgttcatt gccaggaggt gaatggattg acgattaaac gggaaaaatg gctgcgtggc
     2581 gtttactcct ttgtgatact aagtgtaggt gctccgatat ttgtagtagc taatcaattg
     2641 caaaacagct accttcttat cctaactatt ggtgtcctat gccttattgt ctgctgtatt
     2701 cctaaaaaat gggtacgttt gctgctttct ttgccagtaa tgataggttg tttgttttta
     2761 tattttcctt cgaagacatt ttccatatta tggatcttac agtttttttc gtcagttaca
     2821 gaggaattct tccatctatc ccaaggtaaa ctttattatt tgccagaaaa gacagctttt
     2881 tttctcataa tgttgctgat ctatttattt attacattga ccgtggatta cgactattgg
     2941 tacgcaccct ttctttcttt gatggcttac ttcatgatgc ttaccgtttt tcggaaaaaa
     3001 gatttactgt acgaaatgat tgcggtagta actgttttgt tcgtgtatct ggcagcaagg
     3061 caatttttct ccatccggtt gttgtcgaga aacactcgat tccagtcatt gactacagtt
     3121 ctgcttattt gtttcttaac ttttagcgct atcttgcctt tatatcttcc gaaagtacac
     3181 caatctctcg aggaaacgag tgaaccgatc cgagaatatc ttaatgatga agggctttat
     3241 gacacactcc atgagtatca attaaccgga agtgcaagaa caggcttcag tgagaatgat
     3301 gaacagcttg gtggaccttt atatgataat ggcgaagttg tttttacagc gaaccaaaaa
     3361 ggaaatggct attggaagat cgaaaataaa aatcattaca ccggttctgg atgaatagaa
     3421 gatcaggacg cacccatcca accaatccaa gaattccctg ttgacatcac gagctaccaa
     3481 accgttatag aagacaaaga aaacattaca ttgaccctaa aaaaacaagc tttcttacct
     3541 tatccttatg gaaatactac cttgactgat ttttcattag ctagtggaca aacgctttac
     3601 tatttgcctg aaagccagcg attcattcct gtcccgaatg aatcgacacg ttctgcaact
     3661 ttacaaacag aaaggttagt ttttacgaga gaagaattaa aaagcagtca gttaaaagct
     3721 gatgatttag aaaatcagca gacaaatctt caattgccaa aaagtttgcc taaacgtatt
     3781 gaggacttgg cggtcgaatt gaccaaaaat gaagcaactt taattcgtaa agtcgaagct
     3841 gtctaacagt atttaaaaac aagcggaaat ttccgctatt ccaaaacgga taccgggcat
     3901 actccaaaag aacacgatta tgtggattat ttcctctttg attcaaaagt cggctattgt
     3961 gacaactttt caacttctat ggtcgtcctt cttcgaacac ttggtatccc agctcgctgg
     4021 acaaaaggtt ttacaaatgg gctcagaaca ggcacagata atcaagggaa tgaggaatac
     4081 agcattctca atagtcatgc tcattcttgg ccagaggtat actttcctgg atttggctgg
     4141 cttccatttg aaccaacccc ttcatttgcc aatccaatgg gttcacaacc agttgctgaa
     4201 acaaccgaaa ccgtccagcc tgctatcgaa caaccaaatg aaagcgaaaa tatggcagaa
     4261 actatttcca gtgagagatc tgaggtcatt ccatcagaag aaacaacact cacaccagct
     4321 tataaaatcg tcttattcag ccttggagtg tgtttatccg tcttgttgtt ctttctattg
     4381 ttattcagaa aatctctgcg ctggacaagt tatcttatct atcttcattt ttttgcttct
     4441 actaatacga ttgcatatca aaaacttctt aaccctttgg aaacattggt accgcgcaaa
     4501 cccgcagaat ctttgcagga atatagcctg cgaatagaac aagaattcaa gctagatctg
     4561 cgtttcattc gattaactac tcaatatgaa gcagaaatat atggacgaca agcagatccg
     4621 gtaaaattcg ataaaaaaac ctatttagaa ataacgcata taataagaaa aacggtatac
     4681 aaaaaaattc tgacaaaagg gagccgcttc aaaaaataag ataaaatttc tggagaatct
     4741 acttctcgaa tgccgagaac cagctgattg agtatcgatt actcgaaaaa aaggttgtga
     4801 cacgtttttt gtcacaac
//
LOCUS       DACEJR010000126         4655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEJR010000126 DACEJR010000000
VERSION     DACEJR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.134"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IW651_002797"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IW651_002797"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0815460.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IW651_002798"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IW651_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0815461.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IW651_002799"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IW651_002799"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0815462.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1619 a          827 c          996 g         1213 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataag
//
LOCUS       DACEJR010000127         4637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEJR010000127 DACEJR010000000
VERSION     DACEJR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.135"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW651_002800"
     CDS             complement(543..2048)
                     /locus_tag="IW651_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0815463.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW651_002801"
     CDS             complement(2045..2731)
                     /locus_tag="IW651_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0815464.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW651_002802"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW651_002802"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0815465.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1313 a          947 c          764 g         1613 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttggctc
     4441 tataatttat gggactgatg aatcagaatt gattcttcag ccccattttt attttaggta
     4501 ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca actgagagga
     4561 cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag aaagatttga
     4621 tgattacaga ggtctct
//
LOCUS       DACEJR010000128         4595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEJR010000128 DACEJR010000000
VERSION     DACEJR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.136"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..400
                     /locus_tag="IW651_002803"
     CDS             <1..400
                     /locus_tag="IW651_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0815466.1"
                     /translation="ADGLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSI
                     TSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKLYRSLRTASTTIKGMEAI
                     RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(1016..2056)
                     /locus_tag="IW651_002804"
     CDS             complement(1016..2056)
                     /locus_tag="IW651_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0815467.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2405..2731)
                     /locus_tag="IW651_002805"
     CDS             complement(2405..2731)
                     /locus_tag="IW651_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815468.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2718..3506)
                     /locus_tag="IW651_002806"
     CDS             complement(2718..3506)
                     /locus_tag="IW651_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0815469.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3874..4164)
                     /locus_tag="IW651_002807"
     CDS             complement(3874..4164)
                     /locus_tag="IW651_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0815470.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4300..4479)
                     /locus_tag="IW651_002808"
     CDS             complement(4300..4479)
                     /locus_tag="IW651_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815471.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
BASE COUNT         1430 a          785 c          672 g         1708 t
ORIGIN      
        1 tgcagatggt ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta
       61 tgcttttctt aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa
      121 agccccctct attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac
      181 agaacatcgt accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa
      241 gagacgcaat aaattatatc gaagtttacg cactgcctct accacgatta aaggcatgga
      301 agccattcga ggattatata agaaaacccg aaaagaaggc actctcttcg ggttttcggt
      361 ctgtactgaa atcaaggtat tattgggaat cccagcttaa atcatagata ccgtaaggga
      421 ttttattctt tatttaaaac tttgcaacag aaccgtccgt tgcgggtaat accgtaacac
      481 tgaagggcac gcattaaggc gtcatcttaa tgctttcctg taggacactg acggagatta
      541 ccagtctccc gagccttaca ggggcctttc ttttttttgt attttcaatt atcacaattt
      601 atcgtccaaa agtgcagaac gaaaatcaat tcatcacaag catatcagac aattaaattt
      661 aaaacaacta aaaatataca aaaaagtgta attgaatatt agcagcactg cctgaaaaga
      721 agtaatatca ggcttttcag cttggggaaa taagtgaatt acctctcttc atgaattatt
      781 ataactcaaa aaagcacata tgcaaacact agttccctat ttttttgaat gaaaaataac
      841 taaaatatct aacgattatg actttaaaac ctacgtttgt gattgttttc ataaactttt
      901 aatctaaaaa ctccggaata atagctattt atttgtaaca tttgtaataa aattaaaatc
      961 ataaaaaagt tctaatttaa aattgatcat ttttatagtg atcgcttttt atcaactact
     1021 ctagccaatt ttccgtacta actgttggta attcgttaaa tgtttctctt tgttcgtttg
     1081 caagctttgc cgcaatagct ttttcgacaa accaattttt tacataaacg aaaattagat
     1141 tctgcaaatt gtttactttt tcagtttttt gtttttgata agctttcagc aaagtcatat
     1201 ataatccttt gtcagcatca attccataag attccagttc ttcataaaca acaactgttt
     1261 gctctaattc ttcagctttt ttctttgcat tatgaatagt ttggacggtt ttttgagcgt
     1321 cttcgtaagt actagaaaat gtttttatca agtttaatac attttctggt acaaatgtat
     1381 ttatactgtc ctctttcatg atgtcgacga agttatctag taaaacttga ttttgtaact
     1441 gatcagtttc agtgtctatt atgtgtctat tagtgtctat agaattagaa tagaatagat
     1501 tgtctgcgat tttcgcaatt acggttgagt ccttgatatg actggatttt gccgtatttg
     1561 cgattttcgc aattacggtg gtatcattga tttctcgcgg ttttgccgta tttgcgattt
     1621 tcgcaattac ggtggtatca gtattgtctt cctcttccat gattttaaag acgtcttgat
     1681 tcgttacttc tggcttgcct aagtacaata aattaggcat ggattcaatc ttaccgtcac
     1741 gtttatttac tcttcctctc ttctgtttca gaagacctgc tttttctaat tcctttttga
     1801 ttttagatac tttaccttcg cggcattgta acaatttcat taattcttca actgtgaata
     1861 tgaaaaatat attatcatca gaatctaccc agttattttt gactgaataa tcaaatcgat
     1921 ctttaagtag catataagca atttttgcat catttgatag atccatgtat ttttcgttag
     1981 taaaaaatac cttaggcatt tgataatatt tttcacgata aatttctttt gatttgtaat
     2041 aattaaaatc attcatagcc aatcctcttt ctgtaggata gcgttttgtt caaaactatg
     2101 ttacaatgaa ttaaacaaat atatctatcc actatatatt tttctactgc tgataagtcg
     2161 ccaaactttg ttaaagcagt agtctttttt tatctttttt tagtttacct taaaaaataa
     2221 attaaagcaa accgctctat ttcaaggttt aagctcatag taactatatc acgatattac
     2281 aataatatta tatcacgata tcgtaatatt acaatatgaa gataagcgac ttatcagctt
     2341 tgatactact gatttttata aaaaaaatat cacgatatta tgatatcgtg atattgaatg
     2401 taaattaaat tttattttgt ttctttgcta gtttatatcc ttcaatcaag tattcttcag
     2461 gtatggactt taatagcgca tcttctataa cgtctggttt acttttgtct gattcagcaa
     2521 tagtcgtcca atatttgagt gctgacatta gaattggatt tatacgaatg ctatctgctg
     2581 ttcttacact acttccttga tcttttatga ctttacttat atttttcttg gttaattcga
     2641 tttcttctac ttcttttgat ttaaaattct ttttattttt atttcctagt aatctactta
     2701 ctgcagcttg actgcgatta tcttctgcca tcaaaatcac caacttcttt taaatattct
     2761 tctgtaaatg ctttatagtc atcttcagct gttgagggtt tattaaaatt gattagatct
     2821 gatccagcag cttgcgcttc gtcaacggtt attgtcctac gaatataagt tttgaaaatt
     2881 tttgcaccaa tgactctaga aatttcggtt gctgcagacg tcatttcttt agaaatgtta
     2941 taacgcttat tatacttagt aaatagaact cctcgtactt caagcttgtc gttcgtatat
     3001 tcttttaccg tttcaatagt ttcacttaac tttattagtc cttttaatga gaaatacgca
     3061 gcttcaactg gaatgataat ttcatcagaa acagtaaatg cgttgactgt aagcattcct
     3121 aaacttggtg gacaatcaat tataatataa tcatagttgt ccttgattgg ttgaagtact
     3181 cttttaagcc tgtgttcact tcctacttga gtaaattccc tctctgcaga agaaagggct
     3241 atgtcggctg gcaaaacatc aattccttta tagtccagaa tagcatctgt aatgtcagcc
     3301 tcccctttta atacttcata aattgtaggt tggtttgttc catctatatc gaaacctaca
     3361 ttatctgtag catttccctg aggatcaatg tccactaaaa gtacattttt accgtaaaca
     3421 aatttcaaac atgctgctaa attaacgcta gtcgtagttt tagaaactcc acctttttga
     3481 ttggctactg acaatattct tgccattaaa atatccccca ttcatcttat attataatat
     3541 aattatatca taatattgtg atattgcaat atcgtatatg cattatattg taatattgtg
     3601 tgattatttt gttttaaaga tatatatcgg ttcggatata aagattattc atatgttatt
     3661 cgaaattaaa ataatcactt tttattactt taaaattttt tattaatgat aagaaatatg
     3721 ttttgcgagc agcaactaga ataatttaaa cattactaaa ctgtctatat tgttactaat
     3781 aattcccttc ttatagtgca ttttcttaga aaaaaacaat attacgatat catgatatta
     3841 agatatcgta atattataca attgtgattg atattatata tgttttttta tttttttatc
     3901 atgtttaatc ttgaaggaat catattcttt ttctaaagac aggagctttt cataaacatc
     3961 agacatgttt aaactagagg ccaggcttaa atcataaacg aatgaagcag gtaatgattt
     4021 tattctacgt tgtcgggtta tccagcttgc aaccgttcct tgtgggtagg catataactc
     4081 acaaaattgt tctacagtga ttcctaaatt cttgataatg taaacattga tcgggtgcga
     4141 gtaaacaaat gtattgatag acatggtgcg taagcctact ttcttttatt attgcgtttg
     4201 caataatagt ttaacacacg cattattcta gtatctactc ctatttgtaa aaacaaaaaa
     4261 ggattcatag agataactct atagaatccg taaaatacct tatatatata acgggaagat
     4321 ataaaaccta aaattatgat aacctgctgc agcttctaca tagaaagaga agcgctcaac
     4381 accaacctca tttatcaaac gttggccata accccaaatc tcttctgata gctgatcaaa
     4441 ggttatatac tttagctcat attgctggat aatgttcatt ctaatcgact ccatttatta
     4501 tcctggtaaa attcgatatg acgtatttcg tcgtagtgaa ttttgtcttt tccaacgtag
     4561 atccccagct catcactacc taagatcttg ccaat
//
LOCUS       DACEJR010000129         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEJR010000129 DACEJR010000000
VERSION     DACEJR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.137"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            574..1008
                     /locus_tag="IW651_002809"
     CDS             574..1008
                     /locus_tag="IW651_002809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815472.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IW651_002810"
     CDS             2298..3221
                     /locus_tag="IW651_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0815473.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IW651_002811"
     CDS             3237..3776
                     /locus_tag="IW651_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0815474.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          675 c          757 g         1366 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatca atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACEJR010000130         4375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEJR010000130 DACEJR010000000
VERSION     DACEJR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.138"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW651_002812"
     CDS             complement(83..1051)
                     /locus_tag="IW651_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0815475.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2905)
                     /locus_tag="IW651_002813"
                     /pseudo
     CDS             complement(1498..2905)
                     /locus_tag="IW651_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(3127..3489)
                     /locus_tag="IW651_002814"
     CDS             complement(3127..3489)
                     /locus_tag="IW651_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815476.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3493..3801)
                     /locus_tag="IW651_002815"
     CDS             complement(3493..3801)
                     /locus_tag="IW651_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815477.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3810..4229)
                     /locus_tag="IW651_002816"
     CDS             complement(3810..4229)
                     /locus_tag="IW651_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815478.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4222..4362)
                     /locus_tag="IW651_002817"
     CDS             complement(4222..4362)
                     /locus_tag="IW651_002817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815479.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1235 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaaatat
     2101 acctcaactc atcagaaacg ctctcacgct cttttacagc cgtttggcgc aattcagaaa
     2161 tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta ccgacaatat
     2221 cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct tgatttctaa
     2281 aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt tgaaccagtg
     2341 ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca accggcacaa
     2401 tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta taaacagcca
     2461 aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta ttggcaatca
     2521 ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct gctcgatttg
     2581 tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt tccaactttt
     2641 tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt ttttgtttcg
     2701 cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct agattcgtct
     2761 gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc caatcattgg
     2821 ttttctggcg atcagttaac tcacgattat tatgagaaat acttgtattc ttcgttcctt
     2881 ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg ctaataaaaa
     2941 gattgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat tgtgacatgg
     3001 ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag
     3061 gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat
     3121 aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct cactcatctg
     3181 acttttctta ttcggtatca ctccgaataa gccgtcagat tcatcgggat aatctcgttc
     3241 gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt cgtgttgctg
     3301 atcgtcagaa atcgacgata gccaccgctc aaaatcagca aaagtttcaa acccaaaacg
     3361 cttgcgaaga gccttgccca ttgcagcatt ttctttctca atcaagcgtt ttttctgttg
     3421 gcgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat ctgctaaagt
     3481 cattcccata aactacatct ccttttcttt ggaattaata acggaaattt ggtttaactg
     3541 cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc
     3601 taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctagat cagcattgat
     3661 aaccgagttg agaacatcca tcattcgttc aagcatttca ctataattat cttcatccgg
     3721 caaattcaaa taatcagcta accgttcata catcaattca attctatcga ctgtttcttc
     3781 tctcaaatca attttatcca tgtcacacct cacttccaga attgccacca ttttcgattt
     3841 tttaattctt gattttctag taacaactga tcgttttttt gttgctgaag caaagtcaat
     3901 ttttgttggt cacgaatcat ttccgttaat tcagaaatat gcctatcttt ttccgctaat
     3961 tgttcacgca aaaattcatt ttctttttct aacgggaaat cattgcctgc atcattgacc
     4021 ggtaaatcac cagtcaattg accacctttt gaccgataaa ttgaccgcac aacaccaatc
     4081 aattccttgt ctaacatgat aaccccatta tctttatacc gagcaaggtc ataacctgca
     4141 ttgaccagtt gattgcgcac tgtattttta gatataccca actcatcagc aaggtttttt
     4201 atggtcattt tttctggcaa atcagtcatt atcttctccg ttactttcag ctaccccata
     4261 aacatattta acagcatgct tagggataat gattggggaa tcaattttat tatttttcaa
     4321 aatcaactca tactgcgtta catcaagtaa cttaccggac atttttttgc tatcc
//
LOCUS       DACEJR010000131         4362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEJR010000131 DACEJR010000000
VERSION     DACEJR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.139"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            149..1072
                     /locus_tag="IW651_002818"
     CDS             149..1072
                     /locus_tag="IW651_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0815480.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1090..2331
                     /locus_tag="IW651_002819"
     CDS             1090..2331
                     /locus_tag="IW651_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0815481.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="IW651_002820"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="IW651_002820"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0815482.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1502 a          689 c          940 g         1231 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     4321 tttccatttg tttctctaat actcaggaca aaacactgat tt
//
LOCUS       DACEJR010000132         4218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEJR010000132 DACEJR010000000
VERSION     DACEJR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.140"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..210
                     /locus_tag="IW651_002821"
     CDS             10..210
                     /locus_tag="IW651_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0815483.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="IW651_002822"
     CDS             288..962
                     /locus_tag="IW651_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0815484.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1195..1629
                     /gene="dcm"
                     /locus_tag="IW651_002823"
     CDS             1195..1629
                     /gene="dcm"
                     /locus_tag="IW651_002823"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0815485.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1630..2337
                     /locus_tag="IW651_002824"
     CDS             1630..2337
                     /locus_tag="IW651_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0815486.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2327..2617
                     /locus_tag="IW651_002825"
     CDS             2327..2617
                     /locus_tag="IW651_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815487.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="IW651_002826"
     CDS             2876..4060
                     /locus_tag="IW651_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0815488.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4075..4194
                     /locus_tag="IW651_002827"
     CDS             4075..4194
                     /locus_tag="IW651_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0815489.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DACEJR010000133         4215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEJR010000133 DACEJR010000000
VERSION     DACEJR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.141"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            624..3263
                     /locus_tag="IW651_002828"
     CDS             624..3263
                     /locus_tag="IW651_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0815490.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="IW651_002829"
     CDS             complement(3431..4129)
                     /locus_tag="IW651_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0815491.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1221 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttat
//
LOCUS       DACEJR010000134         4199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEJR010000134 DACEJR010000000
VERSION     DACEJR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.142"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1223)
                     /locus_tag="IW651_002830"
     CDS             complement(3..1223)
                     /locus_tag="IW651_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0815492.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IW651_002831"
     CDS             complement(1243..1848)
                     /locus_tag="IW651_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0815493.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IW651_002832"
     CDS             complement(1897..2829)
                     /locus_tag="IW651_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0815494.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="IW651_002833"
     CDS             complement(3254..4111)
                     /locus_tag="IW651_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815495.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1467 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaaga
//
LOCUS       DACEJR010000135         4158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEJR010000135 DACEJR010000000
VERSION     DACEJR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.143"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW651_002834"
     CDS             <1..156
                     /locus_tag="IW651_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0815496.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IW651_002835"
     CDS             175..1452
                     /locus_tag="IW651_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815497.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IW651_002836"
     CDS             1462..2118
                     /locus_tag="IW651_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0815498.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IW651_002837"
     CDS             2118..3494
                     /locus_tag="IW651_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0815499.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3780..>4158)
                     /locus_tag="IW651_002838"
     CDS             complement(3780..>4158)
                     /locus_tag="IW651_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ0815500.1"
                     /translation="WTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIR
                     NEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFK
                     RLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1651 a          471 c          728 g         1308 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     4141 cctcgatact tggtccaa
//
LOCUS       DACEJR010000136         3988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEJR010000136 DACEJR010000000
VERSION     DACEJR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.144"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..2545)
                     /locus_tag="IW651_002839"
     CDS             complement(53..2545)
                     /locus_tag="IW651_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0815501.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2552..3019)
                     /locus_tag="IW651_002840"
     CDS             complement(2552..3019)
                     /locus_tag="IW651_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0815502.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3155..3228)
                     /locus_tag="IW651_002841"
     tRNA            complement(3155..3228)
                     /locus_tag="IW651_002841"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Arg,seq:acg)
     gene            complement(3286..3359)
                     /locus_tag="IW651_002842"
     tRNA            complement(3286..3359)
                     /locus_tag="IW651_002842"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3323..3325),aa:Pro,seq:tgg)
     gene            complement(3381..3454)
                     /locus_tag="IW651_002843"
     tRNA            complement(3381..3454)
                     /locus_tag="IW651_002843"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3418..3420),aa:Arg,seq:acg)
     gene            complement(3465..3550)
                     /locus_tag="IW651_002844"
     tRNA            complement(3465..3550)
                     /locus_tag="IW651_002844"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3514..3516),aa:Leu,seq:taa)
     gene            complement(3567..3638)
                     /locus_tag="IW651_002845"
     tRNA            complement(3567..3638)
                     /locus_tag="IW651_002845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3604..3606),aa:Gly,seq:gcc)
     gene            complement(3650..3722)
                     /locus_tag="IW651_002846"
     tRNA            complement(3650..3722)
                     /locus_tag="IW651_002846"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3687..3689),aa:Thr,seq:tgt)
     gene            complement(3740..3821)
                     /locus_tag="IW651_002847"
     tRNA            complement(3740..3821)
                     /locus_tag="IW651_002847"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3785..3787),aa:Leu,seq:tag)
     gene            complement(3829..3901)
                     /locus_tag="IW651_002848"
     tRNA            complement(3829..3901)
                     /locus_tag="IW651_002848"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3866..3868),aa:Lys,seq:ttt)
     gene            complement(3905..3977)
                     /locus_tag="IW651_002849"
     tRNA            complement(3905..3977)
                     /locus_tag="IW651_002849"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3942..3944),aa:Val,seq:tac)
BASE COUNT         1077 a          922 c          701 g         1288 t
ORIGIN      
        1 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
       61 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
      121 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
      181 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
      241 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
      301 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
      361 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
      421 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
      481 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
      541 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
      601 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
      661 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
      721 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
      781 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
      841 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
      901 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
      961 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
     1021 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
     1081 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
     1141 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
     1201 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
     1261 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
     1321 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
     1381 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
     1441 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
     1501 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
     1561 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
     1621 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
     1681 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
     1741 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
     1801 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
     1861 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
     1921 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
     1981 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
     2041 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
     2101 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
     2161 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
     2221 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
     2281 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
     2341 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
     2401 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
     2461 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
     2521 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
     2581 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
     2641 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
     2701 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
     2761 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
     2821 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
     2881 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
     2941 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
     3001 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
     3061 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
     3121 aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt cgaaccccta
     3181 accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat attcaattta
     3241 aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg aagacaggat
     3301 tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt
     3361 ttaaaaaata aaaaagacta cgcgcccaag aggagtcgaa cccctaacct tttgatccgt
     3421 agtcaaacac tctatccagt tgagctatgg gcgcatattt gaaatgccga ggaccggaat
     3481 cgaaccggta cggtgatcac tcaccgcagg attttaagtc ctgtgcgtct gccagttccg
     3541 ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg ttcgaacccg cgacccccac
     3601 cttggcaagg tggtgctcta ccactgagct atttccgcgt gtttaaagat gccggctaaa
     3661 ggacttgaac cctcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg
     3721 gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac ccccacgccg taaggcgcta
     3781 gatcctaaat ctagtgcgtc tgccaattcc gccacacccg caaaaatatg agccgtacag
     3841 ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc taccaactga gctaacggct
     3901 catatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
     3961 agctgagcta aacctccatg gataactt
//
LOCUS       DACEJR010000137         3937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEJR010000137 DACEJR010000000
VERSION     DACEJR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.145"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..515
                     /locus_tag="IW651_002850"
     CDS             <1..515
                     /locus_tag="IW651_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0815503.1"
                     /translation="RNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSI
                     TNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALMGITISIPPRKNMDKSEK
                     LDHSLLGKQRKTVGTVFSLLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRF
                     EGTLRYSLGY"
     gene            complement(574..2376)
                     /locus_tag="IW651_002851"
     CDS             complement(574..2376)
                     /locus_tag="IW651_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0815504.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(2439..3281)
                     /locus_tag="IW651_002852"
     CDS             complement(2439..3281)
                     /locus_tag="IW651_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815505.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            complement(3297..3518)
                     /locus_tag="IW651_002853"
     CDS             complement(3297..3518)
                     /locus_tag="IW651_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815506.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            complement(3661..>3937)
                     /locus_tag="IW651_002854"
     CDS             complement(3661..>3937)
                     /locus_tag="IW651_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ0815507.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT         1191 a          796 c          565 g         1385 t
ORIGIN      
        1 ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa
       61 aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga
      121 ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg
      181 aagaagcgaa tcttcccaat atcctagggg acaaaggata tattagccat aaaatccatg
      241 aaaagcttgc ccttatgggc attactatat ccattccgcc acgtaaaaac atggataaat
      301 cagaaaaact agaccacagc ttacttggaa aacaaagaaa aacagttggg actgtttttt
      361 ctttgttaga aaaattaggt tgtcaaaatt tcaactcacg ttctgttaaa gggctagaga
      421 gtagatttga aagcatatta ctggcttaca gtgttctatt aagtcgagca caacgacgtt
      481 ttgaaggaac tttgagatat tctttaggat attaaaatta actagcacca cgggtatcta
      541 ttaagaagat cctaggttat cttagattat ctgctaatct ttgtatttcc agcagtatcc
      601 tccagcatgt ttttgaactc catttgcagc atccctaata ctttttgagt taacgccaac
      661 ttcttttaca gcagaagcta tagtatcgaa ttcttttatt attgttccat catcagtcat
      721 ttgaataatc tgtcttccac gcttcttgct aggctcacta atcgcatcct cttttttgtt
      781 cgcttttaga aaatcagatt tttccttagc tatccgctct gcctcttgtt tagcaaataa
      841 tagcttaatt gccttatccc tacgagcctc taaagtacca acatcgtgcc gtgttttacg
      901 cataattcct tcatcaagaa tgagaatttc attatcgtaa tccttaattt ttcgataagc
      961 tttagcataa gaatcatata gcgcaggagc atcatagccg ttatatcgag ctttatcaaa
     1021 aagtgcaata gcttcatcta ctttgccatt tttcctagca gtttctccac gttcccaata
     1081 tggataacct gcatcaaatc ctttctccca attccctata tttcgtatct tgtttaatga
     1141 aactcgatta tgcaacccgt taataattac atcttctcta gatatcactt tttcaatatt
     1201 taggttaact tcagtctcat catatccacg attttttgca tcgaataata actctttcat
     1261 cgctgatttt gaaatagatt tcagcatact aaggctttgc tctgcttctt ttttagtgta
     1321 attactaaca ctcatatatt cattcataga attataacaa tcgctataag cactgaaaat
     1381 ataatttgaa agaggcgtta agtcaaaggg gctacgaaaa tagactcgca catttgttgt
     1441 accgccttca cttacagtac aaggttctat aggtaaatca attccatttg tagcatcttt
     1501 tctaataatg atgtaatttc ccttcttagc aaacttaaac tttaagaatg tatatagaca
     1561 agttacatcg acataattat tactgttttt tcgatacctg atccaactgg tgtcgcatcc
     1621 ttttttctca ataatatctt gaataaaagc acaagcttca agttctttta tgtctggagt
     1681 catcttttct atttgctttt tttgttcttc caacgactcc tcaataatat ctaaaacacc
     1741 ccaaaaaatc tttccgcata tatctgcatc tgattccgct ctatgggcag tgtcatttat
     1801 taatccgaaa tattccgcta cagtctcttg cttatgattt acaatgcctt ttatatgttt
     1861 tctagagaga ctcagagtat caacatactt aattttagca tcatacccaa gtctagaaaa
     1921 tgtgttactt aaaaatccaa aatcgaaact tgcattgtgt gcacacatag tgatttttcc
     1981 atctagtgca tctcctaaaa attctaccaa ctgggggtaa acttcctgct catttggagc
     2041 agagtttagc atttcattag ttatattatt tattgccgaa gctgaggctg caattctaac
     2101 ttttggattg acaagagttc cgaatgattg aattggaact ccttctttaa atagcaccgc
     2161 accaatctct acaattctat ctgaagaaga atttaggcca gttgtttcca catcaaaagc
     2221 gataaaagtt ttacgaaact catctacatt tgatatgtcc acttccaatt tttcagcaaa
     2281 aactttacca gtacgaaata gactagaagc cagttgataa tcaacgtgtt tactaacctt
     2341 tccttgtttt ttctttagaa aatcaaataa tcccactatt atttcctccc ccattaattt
     2401 tatccagaat cacttttacg atgccttttt cttatattct acctattcgc ataccacaag
     2461 aagtgatcta actttcttct tctaaaagga atatgtatat tattttcatc aagtagtttt
     2521 ccaacacatt cgttaaatgc ctcgtaatca aaacttcttc gccacgtatc aatcttattc
     2581 gtagttagtc cttggatata gtgtggaaga gtatttttaa cgataccatc aaagatggag
     2641 taatcatctc tcccacacac ttccacatta tgatatgtac agtacttgga tgcaaatgag
     2701 aacatattaa ctgcaccaat gttccttgcg ataatattta tcaactgagg atctcctttt
     2761 gccaaccgat catcaaaatt tggaatctcc agaattactt tagccaaatc atatagagac
     2821 aacctgctct tatattgaga aagatgtgtt gaatttgtta catcaattac cgcaatcttc
     2881 atcgcaatag tatttaaatc attatgaaga ggaaatgcat tcaaaacatc atgtattatt
     2941 agaccttctt tcccataatt agagcttgca cctacaaact tatgctgttt ctcaatattt
     3001 tcttcagtta aagataagta tttttgttta tggattgttt cacttatagt tttttcttta
     3061 cttgaatcaa gcttaagaac cttgtcaatg caccaatatg ttgttgctcc ttttattggg
     3121 gaaatatcat tctgcttaag atattctttt aataaactca gttgttttcc ttctggaata
     3181 gtaccagatt tcgtttctat gatatgaggt tgtggtttgg aagtaccttc attagcagca
     3241 aatacagcat actcaacatc atttattgtt atgattttca atacaaatta cacctcctaa
     3301 ttacctatct ttatttcatt gccatcaggc aatataaaag cctgcacaaa cttgacacct
     3361 agcacatcag caatcgcttt caactcatct aaggttaccg tttctcgttg taatttctta
     3421 ttgaaattct gtggagtctg tccaatacgt ctagcaagtt cggaaacact tatattcatt
     3481 tgctcacaca gttctttaat catttctgcc gtagtcatac agcacctcca agtttacgct
     3541 aagaatatta taaatcattt ggtttataat aacaatagtt attaaaatat tattataatc
     3601 agcaagcaaa agaaaaccca cgactctact gtagaatcat gggctattta cttctggttt
     3661 tcatatttcc atttcgatgc cagatttgaa ggatatgacg aagtgttctt catagactgt
     3721 aacgctctgg attatcttcc ttacaagctt atcatcgtac tctaaggtac ggaatttgtt
     3781 cttgcggata aattcaatca gctcattgat tcgctcgttc tcgccactga gggaggcatc
     3841 ttctactaaa aggagctgtc gcttgtctct cagctcatca atctcatctg ctagatgttc
     3901 ataatcttga cccttattag ccagcttgat gagttct
//
LOCUS       DACEJR010000138         3883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEJR010000138 DACEJR010000000
VERSION     DACEJR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.146"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW651_002855"
     CDS             82..3123
                     /locus_tag="IW651_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0815508.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="IW651_002856"
     CDS             3313..>3883
                     /locus_tag="IW651_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ0815509.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACEJR010000139         3800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEJR010000139 DACEJR010000000
VERSION     DACEJR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.147"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..637)
                     /locus_tag="IW651_002857"
     CDS             complement(23..637)
                     /locus_tag="IW651_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815510.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            951..1373
                     /locus_tag="IW651_002858"
     CDS             951..1373
                     /locus_tag="IW651_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815511.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1383..1586
                     /locus_tag="IW651_002859"
     CDS             1383..1586
                     /locus_tag="IW651_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815512.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1797..2381
                     /locus_tag="IW651_002860"
     CDS             1797..2381
                     /locus_tag="IW651_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815513.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2550..>3800
                     /locus_tag="IW651_002861"
     CDS             2550..>3800
                     /locus_tag="IW651_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0815514.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         1283 a          602 c          716 g         1199 t
ORIGIN      
        1 ataataaaag cagcatgatc aactatttgt cattgaccat gctgtctttt tttattctat
       61 acacggtttg tctcgtaaca ttgtagtctt tagcaatctt agcaatcgca acaccatttt
      121 taagatcttc aacgatgctt ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac
      181 tgtataattt gggccgccct ttgtagacgc cgtttgcttt tgcgatctta attccttgtg
      241 cctggcgacg tttcgattca gtccgttctt gttcagcgat catggccaaa atttggatga
      301 tcagatcctt aatgaattta tccaggagag gattgccaat tgcttcagcc atgatcggta
      361 aactagtaat gatcagcttc acatttttct tcttcaagta attgactgaa gtgatgatct
      421 cgtcataatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
      481 cgtaatctaa tgcttcttgg aaaacaggtc gatgagtaat cgttgcgcca gattgtttct
      541 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aatctggcgt tgttcatttt
      601 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
      661 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
      721 tctatttagg gtaaacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca
      781 cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg
      841 attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta
      901 agatttatta agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata
      961 agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga actacaattc
     1021 ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa
     1081 ctcctttaga taggaacgga atagccattg gtaacccaac taacgactta actgttactg
     1141 aaaattattt aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga
     1201 caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt
     1261 ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat
     1321 cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt
     1381 ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa
     1441 ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa
     1501 aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc
     1561 aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct
     1621 ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct
     1681 ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca
     1741 gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga
     1801 aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc
     1861 taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc
     1921 gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc
     1981 gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg
     2041 agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta
     2101 caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc
     2161 tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc
     2221 ctgaaaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac
     2281 ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg
     2341 caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg
     2401 aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct
     2461 actgataact tattctcaaa tatttagcta tttcttcttt acaacatcga acatatgttt
     2521 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
     2581 attacttttt aatcgatatg aaaagctttt acgcaagtgt cgaatgtata gaaagaaatt
     2641 tagatccatt aacaacagaa cttgttgtta tgagtcgatc tgataatact ggttcaggat
     2701 tgatattagc ttcttctcct gaagcaaaaa agcggtatgg tattacaaat gtgagtagac
     2761 cacgtgactt accacaacca tttcctaaaa cactacacgt tgttccacca cgtatgaacc
     2821 tatatatcaa gcgaaatatg caggtaaata atattttcag aagatatgtg gctgatgaag
     2881 atctactgat ttactcgatc gatgaatcga ttttaaaagt gacaaaatca ctgaatcttt
     2941 ttacgactga agaaacacga agtcagcgaa ggaagaagct cgctcaaatg atccaagaac
     3001 gtattaaaga agagttaggt ttgattgcta cggtaggcgt cggcgataat ccccttttgg
     3061 ctaaattagc cttagacaat gaagcaaagc ataatgaagg atttattgcg gaatggacgt
     3121 acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat gacggacttt tggggaattg
     3181 gttcgcgaat gaaaaaaagg ctcaaccaaa tgggaatttt gagcattaga gaccttgcta
     3241 actggaatcc ttatacgatt aagaatagat taggtgtgat tggattacag ctgtacttcc
     3301 atgctaatgg gatcgatcga acagatatag caatcccacc agaaccgaca aaggaaaaat
     3361 catatggaaa ttcacaagtg ttaccacgag attatactag aagaaatgaa atcgagctgg
     3421 tggtaaaaga aatggcggaa caagtagcga tacgcattag gcagcataac tgtaaaacag
     3481 gttgtgtcca tttacacatt ggcacgtcga tcttagaaac gagaccaggg ttttcacacc
     3541 agatgaagat ccctatcaca gataatacaa aggaattgca gaattattgt cttttcttgt
     3601 ttgataagta ttatgaagga caagaagtaa ggcatgttgg aataacctat agcaagcttg
     3661 tgtacaccga ttcgcttcag ctggatttat ttagtgatcc acaaaagcaa attgacgaag
     3721 aaaatttaga taaaattatt gacaagattc ggcaaaaata tggttttacg tcgattgtac
     3781 atgcttcttc aatgttagaa
//
LOCUS       DACEJR010000140         3393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEJR010000140 DACEJR010000000
VERSION     DACEJR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.148"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW651_002862"
     CDS             179..775
                     /locus_tag="IW651_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0815515.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW651_002863"
     CDS             899..2698
                     /locus_tag="IW651_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0815516.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IW651_002864"
     CDS             complement(2976..3188)
                     /locus_tag="IW651_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815517.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          947 a          593 c          610 g         1243 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagtaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttt
//
LOCUS       DACEJR010000141         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEJR010000141 DACEJR010000000
VERSION     DACEJR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.149"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..588
                     /locus_tag="IW651_002865"
     CDS             37..588
                     /locus_tag="IW651_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ0815518.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IW651_002866"
     CDS             1264..1530
                     /locus_tag="IW651_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815519.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IW651_002867"
     CDS             1588..2100
                     /locus_tag="IW651_002867"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0815520.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IW651_002868"
     CDS             2097..3134
                     /locus_tag="IW651_002868"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0815521.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3294..3376
                     /locus_tag="IW651_002869"
                     /pseudo
     CDS             3294..3376
                     /locus_tag="IW651_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1242 a          576 c          669 g          889 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac accgaccatc
     3301 gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct acaacgatta
     3361 aaggcatgga agccat
//
LOCUS       DACEJR010000142         3287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEJR010000142 DACEJR010000000
VERSION     DACEJR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.150"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..2198
                     /locus_tag="IW651_002870"
     CDS             99..2198
                     /locus_tag="IW651_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0815522.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2394..3257
                     /locus_tag="IW651_002871"
     CDS             2394..3257
                     /locus_tag="IW651_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0815523.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
BASE COUNT         1342 a          565 c          660 g          720 t
ORIGIN      
        1 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
       61 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
      121 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
      181 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
      241 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
      301 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
      361 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
      421 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
      481 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
      541 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
      601 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
      661 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
      721 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
      781 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
      841 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
      901 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
      961 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
     1021 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
     1081 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
     1141 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
     1201 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
     1261 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
     1321 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
     1381 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
     1441 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
     1501 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
     1561 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
     1621 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
     1681 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
     1741 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
     1801 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
     1861 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
     1921 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
     1981 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
     2041 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
     2101 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
     2161 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
     2221 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
     2281 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
     2341 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
     2401 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
     2461 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
     2521 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
     2581 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
     2641 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
     2701 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
     2761 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
     2821 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
     2881 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
     2941 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
     3001 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
     3061 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
     3121 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
     3181 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
     3241 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagct
//
LOCUS       DACEJR010000143         3278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEJR010000143 DACEJR010000000
VERSION     DACEJR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.151"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            210..476
                     /locus_tag="IW651_002872"
     CDS             210..476
                     /locus_tag="IW651_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0815524.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="IW651_002873"
     CDS             503..802
                     /locus_tag="IW651_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0815525.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="IW651_002874"
     CDS             976..1527
                     /locus_tag="IW651_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0815526.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="IW651_002875"
     CDS             1673..1966
                     /locus_tag="IW651_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815527.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="IW651_002876"
     CDS             1959..2255
                     /locus_tag="IW651_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0815528.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..>3278
                     /locus_tag="IW651_002877"
     CDS             2330..>3278
                     /locus_tag="IW651_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0815529.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDG"
BASE COUNT         1229 a          451 c          655 g          943 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga agctactatt caattaatat tgtaacggat gatgatggaa acacgaccat
     3241 ggctggtgga aaacaatttc gtgtttatac agatggaa
//
LOCUS       DACEJR010000144         3275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEJR010000144 DACEJR010000000
VERSION     DACEJR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.152"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..334
                     /locus_tag="IW651_002878"
     CDS             <1..334
                     /locus_tag="IW651_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0815530.1"
                     /translation="ILKSIDPGGVMLAELMDARVDIQGVRHASISERLLADMDYLYQK
                     LRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPIEEGALIIATVDDPKQNVF
                     TLEKVGVI"
     gene            334..951
                     /locus_tag="IW651_002879"
     CDS             334..951
                     /locus_tag="IW651_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815531.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            955..1404
                     /locus_tag="IW651_002880"
     CDS             955..1404
                     /locus_tag="IW651_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815532.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            1404..1928
                     /locus_tag="IW651_002881"
     CDS             1404..1928
                     /locus_tag="IW651_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815533.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITRDPWPA"
     gene            1956..2363
                     /locus_tag="IW651_002882"
     CDS             1956..2363
                     /locus_tag="IW651_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815534.1"
                     /translation="MKTIYKVLYPLGYEEHEVEDNFPTGLPFVEVPPILFEKKEDETD
                     EAFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKANEELTTK
                     AEGLAQINSKTMLTSLQNTKDIATIKEQLDGGK"
     gene            2365..2502
                     /locus_tag="IW651_002883"
     CDS             2365..2502
                     /locus_tag="IW651_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0815535.1"
                     /translation="MYSYDDIKMMFDWNCFTADQVRQFVPLCITDEEAEKIINNEESA
                     S"
     gene            2540..2833
                     /locus_tag="IW651_002884"
     CDS             2540..2833
                     /locus_tag="IW651_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815536.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            2830..3054
                     /locus_tag="IW651_002885"
     CDS             2830..3054
                     /locus_tag="IW651_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0815537.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGTFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            3051..>3275
                     /locus_tag="IW651_002886"
     CDS             3051..>3275
                     /locus_tag="IW651_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0815538.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSN"
BASE COUNT         1168 a          510 c          700 g          897 t
ORIGIN      
        1 tattttgaag tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt
       61 tgatattcaa ggagttcgtc atgcgtctat ttcagagcgc ttgctggcag atatggatta
      121 tctatatcag aaattacgag caacactttt cacgattgaa tacggtgaga ttgaagtgac
      181 tgatattttg caggatgatc tcttttcaga taatcacgaa gtcgaaaaag ttggaactgt
      241 agaatttcca attgaagaag gagctttgat tattgcaact gttgatgatc caaaacagaa
      301 tgttttcact cttgagaaag ttggggtgat ttgatggtta aagtaaagag aatgatggag
      361 actgatgaat ctggtgttaa acgtcaattt atgccaatta cacatgtttc tgcagtttta
      421 ggcttagaaa aaatgatatc aggacaatca aaagttcttt cggttaatgg taaatatggt
      481 gcagtcattt taacgaaagc tgatttagga ttagagaatg caattactga actaccttat
      541 gcaagcgaga caagcgatgg tattcttact gctgaaatgt ttcaaaagat cgtaaatgga
      601 gaaggtggaa catatatttt gccgattgct actcctgaac aattaggagg tataaaagtt
      661 ggggaactct tagaagttac tgaagaaggt gttttatctg caactaagca aacagacttc
      721 aatttctctg aagaattaaa gggaaaatta gaatctctaa aaatattaaa agcaggtgca
      781 aatatatcaa ttgcagaaga tggaacaatt agttctacag gtggtagtgg gactggtggt
      841 gttaatcagt catatgtaga tcaaaaattc caagaagctg taaatcaagc agaaagttac
      901 acaaacgaaa gaattccaaa ttttactttt gaaaaaatcg gggaggtata gaaaatgaca
      961 gatatcgtaa aaataaaaca aagcggtgta caggtttatc cacaaactca ttggaatgct
     1021 atagaaggca aaccaacaac ggtgaaggga gataaaggcg atcctggcca agcagcaaca
     1081 attaccatag gaaccgtttc tagtggttca accgcttctg ttacgaatgt aggaacatca
     1141 tctgcagcta gatttaattt tgttttacca aagggagata aaggtgatcc tggaataaat
     1201 gccacaacta cagctgtagc tacaactaca gcaaatgggt tgatgtcgtc cacagataaa
     1261 actaagttgg atggaattgc agctggagca cagaaaaatc caggtaatgc tacaactacg
     1321 acagcaggtt taatgtcagc aaccgataaa gtgaaactcg atggattaac caatattaca
     1381 tttgagaaag tggggacggt ttaatggctg atattgtgca attaaaagaa gacggagttg
     1441 ctaagtattt gaaaactcat gccaaaggaa ttgatggtgt ggagggtgtt ttagtaaaag
     1501 ccacaggtaa tgaaacggtc ttaggaacga aaaattttaa agacggattg caattcaatg
     1561 gattgcctgt gcaagcaggt atgatcgagc gagctatcac actagctgat cgaagtgata
     1621 caacaaacgt aactgatgtg aatggaaaaa taatcagaat cggaaacatc gtatttctta
     1681 cttttaattt taaatgtggc acatggcccg aaggatcaga aactcgttgg attttaaaaa
     1741 tcccggacgg tttcaaacgt gatcaaggct atccagcgca aaccgcgctt tcactagtta
     1801 gaaatgcaag tcagccagcg gatgcacgtg cattcattga tcaaagtagc atcatacaag
     1861 ccaaatcagg ttcaggtagt tcatatattt caggtatgtg gatcactcga gatccttggc
     1921 cggcttaaca gattactaat tggaggaaga gtaaaatgaa aacaatttat aaagtattat
     1981 atccactagg ttacgaagaa cacgaagtag aggataattt cccaactggt ttaccttttg
     2041 tagaagttcc acctattttg tttgagaaga aggaagatga gacggatgaa gctttcggaa
     2101 gaagacaaca gtcgcaattc tttaacttca ctgaaaataa gtgggaagaa gcagttacac
     2161 aagattattc aaaaaaatta gaactactag aaaatttatc tataggttta caggtagata
     2221 atgctgcatt gaaaaaagca aatgaagagc ttactacaaa agcagaagga ctagcacaaa
     2281 ttaattcgaa aaccatgctg acatcccttc aaaacacaaa agacattgct actatcaaag
     2341 aacaacttga cggaggaaaa taatatgtat tcttatgatg atatcaagat gatgtttgac
     2401 tggaattgtt ttactgctga ccaagttcga caattcgttc cactatgcat tacagacgaa
     2461 gaagcagaaa aaattattaa caatgaagag agcgcatctt aattgatgtg ctttttattt
     2521 tgattcaagg agttgtcaca tgattaattt aggggaatgg ggagcgatag caggatcaat
     2581 aaccgctatc gtttctttga ttttattagt aataaaacca attactgcat ctttctcgaa
     2641 gattactgag actctttcaa aagtaagcca caatttagat ttgctgacta aagatttaga
     2701 atcgagcaaa tcagatcgat tgatgattca tgaagaacta aagaaacacg atgaaagatt
     2761 agatacacat acagaaaaat tggtggaaca cacgcaacaa atcaaaactt tgtttaggga
     2821 gagaagaaaa tgaataataa aacgttcgaa gtactaaaat ggttcgcact ggtaattatt
     2881 cccgcactag ctactttcgt ggggttagtt ggtaaagcgc tcaattggca gtacacagat
     2941 atctgtgttg tcatcattac tggttttggc acgtttttag ggagtgtgtt gggtgtatca
     3001 aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag gaggaaacaa atgaaaaaga
     3061 aaattactat tactgcgatg agcctgttaa cggctctttt tttattaccc attaatacgt
     3121 ttgcttatac tattaatgac gagtataatt tagcgccgaa tcaaggagac tccagattag
     3181 caattcctaa caaaattatt ttgcacgaaa ctggaataga tgcaccagca agaaacgtag
     3241 ccgccaacat gaaaaataat tataacggaa gcaat
//
LOCUS       DACEJR010000145         3231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEJR010000145 DACEJR010000000
VERSION     DACEJR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.153"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            183..1137
                     /locus_tag="IW651_002887"
                     /pseudo
     CDS             183..1137
                     /locus_tag="IW651_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1154..2178
                     /locus_tag="IW651_002888"
                     /pseudo
     CDS             1154..2178
                     /locus_tag="IW651_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2184..2663
                     /locus_tag="IW651_002889"
     CDS             2184..2663
                     /locus_tag="IW651_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0815539.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2679..>3231
                     /locus_tag="IW651_002890"
     CDS             2679..>3231
                     /locus_tag="IW651_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0815540.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          979 a          539 c          751 g          962 t
ORIGIN      
        1 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
       61 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
      121 tggacggtat tactgatata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
      181 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
      241 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttgggctttg
      301 atggactgaa agtagatgac aagagctgga cagtgtggaa ttggccacaa ggtgtcggtc
      361 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
      421 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
      481 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
      541 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
      601 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
      661 tgacagtgct tcctttggca aaaatcggga aactgctggt tgacgattgc tattccagag
      721 atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca
      781 cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt atggcatacg
      841 ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt
      901 attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt cgcttatcgt
      961 gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc
     1021 gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac agcaatgcct
     1081 tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta ttgttaaaag
     1141 aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc ttagcctagg
     1201 tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga
     1261 cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt aattgatgca
     1321 atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca gatccagtat
     1381 ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca gtcaggagca
     1441 gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt
     1501 ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat ggataatgaa
     1561 gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg attgacctat
     1621 tatgattctg gtcagctcag cttttatatg gtagacggtc cgatccatac gccggaagat
     1681 ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt
     1741 ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct tcaatccaat
     1801 ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca tggcggggta
     1861 gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat catgaatgat
     1921 gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa
     1981 tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa agctttagca
     2041 caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg agaggcagtt
     2101 gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta tcaaaagatc
     2161 caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta gagatcctgg
     2221 gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt tccagaacgg
     2281 ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt gtctggtttg
     2341 ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta acggttttaa
     2401 gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc
     2461 tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg
     2521 ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca gtacctgtat
     2581 cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta agcaacaagc
     2641 cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa gccggattag
     2701 tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct ataagtattg
     2761 cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct gcttttagtt
     2821 cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt ccgtttttcg
     2881 ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg gattttgcga
     2941 tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta gggaatgctt
     3001 tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt ggagtgttga
     3061 tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt
     3121 cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat tcacttgttg
     3181 ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc g
//
LOCUS       DACEJR010000146         3109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEJR010000146 DACEJR010000000
VERSION     DACEJR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.154"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1191)
                     /locus_tag="IW651_002891"
     CDS             complement(106..1191)
                     /locus_tag="IW651_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0815541.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1511..1918)
                     /locus_tag="IW651_002892"
     CDS             complement(1511..1918)
                     /locus_tag="IW651_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815542.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(1937..2755)
                     /locus_tag="IW651_002893"
     CDS             complement(1937..2755)
                     /locus_tag="IW651_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0815543.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          924 a          482 c          424 g         1279 t
ORIGIN      
        1 ctccggaata ataactattt atttgtaaca tttgtaataa aattaaaatt gtaaaaaagt
       61 tctaatttaa aattgatcat ttttatagtg ttcgcttttt atcaactact ctagccaatt
      121 ttccgtacta actgttggta attcgttaaa tgtttctctt tgttcgtttg caagctttgc
      181 cgcaatagct ttttcaataa accagttttt tacatacaca aaaatcaaat tctgcaaatt
      241 atttactttc tcagtctttt gtttttgata agctttcagc aacgtcatat ataatccttt
      301 atccgcatca attccgtaag attcaagttc ttcatacaca actaccgtct tctcaagttc
      361 ctcggcttta tgttttgctt tgtgtattgt ggtgacggtt tgttgggctt cttcgtaagt
      421 gttagaaaat gtttttatca agcttaatac cttttctggt acaaatgtgt ttatgctgtc
      481 ttctttcatg atttcaacga agttatctag caaaacttga ttttgtaatt ggtctgtttc
      541 agtgtctttt aagtgtcttt tagtgtctaa agatttatta atataataat cttgtgcgat
      601 ttccgcattt ccagtgttat caagggtttc agctgttttt tctcgacgtg cgatttccgc
      661 atttccagtg ttatcaaggg ttttagcagt ttcctctcga cgtgcgattt ccgcattccg
      721 agaagaggtt gatttatcaa tagtttcgct taaatcgtca tcgaaagaat tttgaataaa
      781 aagatcatcg gcacttattt caggttgaag taaatagtat ttattttttt catttttttt
      841 cgtttttggg ttaaattctc cttttacgac tgaaatcaaa ttatatgttt ctaactcgtt
      901 taaaatggtt tttactttgc cttggtagca atttaatttg tccattaggt ctgcttgtgt
      961 aaattccaaa tataatagac catcatcgtc gatccatttt tcatgtaagg ctctacgaaa
     1021 ttcattttta aaaagcatat aggctatttt tgagctgtct ttcattttag agtatttaga
     1081 attctgaatt aaaaaactag gaaatttata atatttttca atataaacat tcgttgcttt
     1141 atatccccaa tttttaatta tcattttttg agattgattt tctgtagaca ttaaatttct
     1201 ccttcttctt agaaacttgt atatattcag gattttcaac aagtgaacta taccatgtat
     1261 taagggtgaa taaaacactt tcagtattta aggtataagt tatatattaa aaaaataagt
     1321 ataagttata ctttaaatac aaataatgta taacttatac tttaaataat aattttataa
     1381 tgttgattta atcgcaaatt tacacgttgt ttattaagtt ataccttaaa tatttaatgt
     1441 ataacttata ctttaatatt catttatcta gtataactta tactttaatt gaataccgca
     1501 tgaaaaaaaa ttattttggt aatttttcaa actcttcttg tatttcttct cttatgtgtt
     1561 cttcttttgc tactcctagt ctatgctgga tatatgagtc tgctaatgta ttaacaattt
     1621 tttcgaaaga aattctccct ttttcacttt gtaactccgt catatagtct tgtaaaattg
     1681 agagtttgct atttaatgcc ggagagatct ttttaggaac tgataccttg tttcttttat
     1741 caggaattga aactggtcgg cctctttttt tcaatgtaat atttcttgtg ttactcaatg
     1801 cactattgct aaagttatat tcaccgctta ttttcacatc ttggcgtttg ttattgggta
     1861 ataatggctc aaattcacca ttctccatca ttttaggaat atcaaaatct ttaggcatta
     1921 cttatgcaac tccttattat ttatttaaaa actcttgtct agcatctagt tctgccaaaa
     1981 tatttataaa tacttggtgt gctttataat cccattgatc aaaatttcca tttttgtatt
     2041 ttcttaaatg tataccaatt ttagaatacc ttctcaatct tttttgataa tgaatgagat
     2101 ttttggatac cacggttccg tatttttcaa ttgcttcttc taggatttct tcatcaaaat
     2161 catcatttgg ttctaccata caaggtacaa tatctataat ttgcaaatca agatcgtatc
     2221 tatcgaccat gaatttttga taatttacat atttgttgac tccctctaag gattcttcag
     2281 ctgtttgaaa tgaaattatc gaatagtctg aagctgccat agcattatcg gaaaattcgc
     2341 taatggttgg tggtacatca ataaatattt cttgataatt gttctttatt ggaagaagta
     2401 attttttcaa gacagtaact tggtctaatt cattttcatg tttattagca aaagcggaga
     2461 atgatcgaaa actagtgtca catgccagaa ggtcaagatt ttcatttacc ttaataatac
     2521 tacttgctaa ttctccattt gcgacaccat ttgttatagt ggttttgata tcggttatac
     2581 cgaaagttct tgctatgact tgtgttaagt tcgcttgagg gtctaaatca ataataagcg
     2641 tttttctatt tcttatcatt gcattgtcat aagcaagcat gctgactatc ttagttttac
     2701 ctactcctcc tttaaaattg ccaactgtaa atgtagtccc ttgaaaagta tccatgtaaa
     2761 tcctcccaat ctttaattga taattaaagt ataacttata tatcggaaat ggtcaacatt
     2821 aaaagtataa cttaaataat aatatctaaa aacgtttgga tatagcggtt tttaataata
     2881 aaagtataag ttataccata tatatttatg gtataactta tacttttatt attaaaggta
     2941 taacttatac cttaaatatt gcttttcgaa aatagctatt ctagtctatc tgtttcttta
     3001 cttttcgctg ctgatttgaa ttgaattgat catattcatt ttctagtatt aataactttt
     3061 cgtatacatc ggtcatattc atactagaag caagacttag atcatatac
//
LOCUS       DACEJR010000147         3045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEJR010000147 DACEJR010000000
VERSION     DACEJR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.155"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..1173
                     /locus_tag="IW651_002894"
     CDS             205..1173
                     /locus_tag="IW651_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0815544.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="IW651_002895"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="IW651_002895"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0815545.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="IW651_002896"
     CDS             2203..2811
                     /locus_tag="IW651_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0815546.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          904 a          583 c          731 g          827 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaaga ggttctgttg caaagtttta
     2941 aataaagaat aaaatccctt acggtatcta tgatttaagc tgggattccc aataatacct
     3001 tgatttcagt acagaccgaa aacccgaaga gagtgccttc ttttc
//
LOCUS       DACEJR010000148         3016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEJR010000148 DACEJR010000000
VERSION     DACEJR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.156"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW651_002897"
     CDS             complement(106..1053)
                     /locus_tag="IW651_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0815547.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW651_002898"
     CDS             1299..1763
                     /locus_tag="IW651_002898"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0815548.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW651_002899"
     CDS             complement(1825..2868)
                     /locus_tag="IW651_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0815549.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          996 a          598 c          577 g          845 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttct
//
LOCUS       DACEJR010000149         2863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEJR010000149 DACEJR010000000
VERSION     DACEJR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.158"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..2378
                     /locus_tag="IW651_002900"
     CDS             6..2378
                     /locus_tag="IW651_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815550.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..>2863
                     /locus_tag="IW651_002901"
     CDS             2439..>2863
                     /locus_tag="IW651_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815551.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          456 c          621 g          642 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gctgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttaaaacc tgaagactat ccgaaaatct
     1501 ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggaa tacaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacggg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acttcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagagaa taagaatatt gtgcccgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgc
//
LOCUS       DACEJR010000150         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEJR010000150 DACEJR010000000
VERSION     DACEJR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.160"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..89
                     /locus_tag="IW651_002902"
     CDS             <1..89
                     /locus_tag="IW651_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0815552.1"
                     /translation="LYQCPTCGKKYTLSYGDYILIDEKGKKR"
     gene            89..340
                     /locus_tag="IW651_002903"
     CDS             89..340
                     /locus_tag="IW651_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815553.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            521..793
                     /locus_tag="IW651_002904"
     CDS             521..793
                     /locus_tag="IW651_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815554.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            1238..1417
                     /locus_tag="IW651_002905"
     CDS             1238..1417
                     /locus_tag="IW651_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815555.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1476..1832
                     /locus_tag="IW651_002906"
     CDS             1476..1832
                     /locus_tag="IW651_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815556.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1016 a          386 c          534 g          848 t
ORIGIN      
        1 gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc gattatattt
       61 taattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat tgactgtaaa
      121 agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagtttt
      181 acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac cgactatgct
      241 atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acttggaaac
      301 ttatgaaaac gaatattggt taaccaaatt agagaactaa aaaggagaaa aaaatatggc
      361 tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga
      421 ggatatgacg ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt
      481 gtcggtagct gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta
      541 ctatcgaaca ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact
      601 atgcttggaa gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga
      661 actaacgtat gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt
      721 agattatttt gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa
      781 cgaaaatgaa taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga
      841 aaaaaaggtc aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa
      901 cgcctagtaa ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg
      961 cttaggtggt gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca
     1021 tttgaacaag attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga
     1081 tagtgcagct gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat
     1141 cttttggcgg tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt
     1201 tttcataggg ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa
     1261 acggttaccc gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa
     1321 gtatggcaaa agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga
     1381 aaaagcagga tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat
     1441 ataagtgata tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt
     1501 acaaattaaa gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc
     1561 atttgtatag agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag
     1621 ctggggatcg atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc
     1681 tttggcttat tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact
     1741 tagataagct aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt
     1801 tagaaagtga cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat
     1861 tattttatta agaatgatgg aatataagga taaggttacc aacataacga tcattacttc
     1921 tctgatatgc tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat
     1981 actgcaagcc ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat
     2041 atataagcta tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag
     2101 ttatcaaaaa gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag
     2161 taggatttga catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg
     2221 gagataagat gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac
     2281 cgcaactgaa gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg
     2341 ttaccttaaa atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga
     2401 tggggtacgt gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat
     2461 aaatttagac atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat
     2521 tatagtaatg attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa
     2581 aattgagaaa tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat
     2641 gaagcttacc aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc
     2701 tttgaggaga tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg
     2761 cggtggaggt aaaggttttt aata
//
LOCUS       DACEJR010000151         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEJR010000151 DACEJR010000000
VERSION     DACEJR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.161"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW651_002907"
     CDS             <1..197
                     /locus_tag="IW651_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815557.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW651_002908"
     CDS             314..811
                     /locus_tag="IW651_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0815558.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW651_002909"
     CDS             898..1290
                     /locus_tag="IW651_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0815559.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW651_002910"
     CDS             1274..>2778
                     /locus_tag="IW651_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0815560.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEJR010000152         2775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEJR010000152 DACEJR010000000
VERSION     DACEJR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.162"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..129
                     /locus_tag="IW651_002911"
     CDS             <1..129
                     /locus_tag="IW651_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0815561.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..678
                     /locus_tag="IW651_002912"
     CDS             130..678
                     /locus_tag="IW651_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815562.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            938..1666
                     /locus_tag="IW651_002913"
     CDS             938..1666
                     /locus_tag="IW651_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0815563.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1696..>2775
                     /locus_tag="IW651_002914"
     CDS             1696..>2775
                     /locus_tag="IW651_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ0815564.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT         1010 a          371 c          593 g          801 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tccaattgtt ccagaattag atgatcgtgg agaaataata
      601 agaactacca gtcagataaa acaagatacc gaagactcac tgcgcaaagg gtggggaatg
      661 aataataaag ttagataata atcaaagaag aactaagtaa ctacctttaa acggtagtta
      721 cttagttctt ctttgatttg cgtgaaatag aggggattta tttttctggt tacgtgatta
      781 aaaaatccaa aaaatgagca taggaacggt tatgatttta gggtgtgttt taatgagata
      841 ttgtgatttc attaacaagt gtatgataat ctgttgttga gttagtaaat aagtaataga
      901 gctaactcta aaataaaact attaggagga gatgtaggtg aaaaagttta aaaagaaaat
      961 atatttagga ttggttgttg gggtgagtat gtttgggttc atggctcata ctaaacaagt
     1021 aactgcagat actaaccaaa aagaaaggat tatgagccaa ttagatgact ctcaaataga
     1081 gtatgtaccc aatgagctaa ttgtcagttt aaaggctaca caaagaattt caacagagaa
     1141 aaataaaccg caaaatttat tgggtgtaaa agttatggtg gaaaaagatt tgactcttag
     1201 gacaaatgaa agcgattata ataagcgaga tcaagtatta ttagtgaaat tgaacgaaga
     1261 aattagtgtg gaagaggcag taagaacatt taatgcttct gatatggtca attatgcaga
     1321 gccaaactat gtggttgaac tagagccgtt gaatgatgtt gaggagccaa atacaactga
     1381 ttctttagga aatcgatcaa ttattcttaa cgatcctaat tggtgtaaac aagctaattt
     1441 aagagagatt agcatgccat ccgcttggga agaaaccaag ggttctaagg atgtgaaggt
     1501 agcggtcatt gattcaggaa ttgattatca acatgaagat ctttctggaa atgtagatga
     1561 aagtttggga tacgattttt ttgacgatga tacagatcct atggatacta tgggacatgg
     1621 acacatattg ccgggattat tggtgctcaa acaaacaacg aattaggtgt tgctggaata
     1681 aattgggatg ttacgatggt acctatacgt gtatttggta gtagtggagg taccagtttg
     1741 gcaatcttaa ctgaagctat taattatgca tcccaaaaag ggtttccaat tatcaatttt
     1801 agtataggta gtgttgttta tagcagtaca gaaaaagcaa ttaaaaacta tcctggatta
     1861 ttcgtagcag cagctggaaa taatgggata gatatgactg ataaaccttt ttacccagct
     1921 tctttggata tacctaatat tattactgta ggaaattcta gtggttatga acgaagttct
     1981 gattcaaatt ataggaaaac aggtgttgat ttattcgcta gagggtttcg tgtttttagt
     2041 acatttctag gaagtaaata cacgtatatg tcaggtacct cgatggcaac acctcatgtc
     2101 actggtagtg ctgcattagc gttgtcacta gatcctgatc tatctactga tgaattaaaa
     2161 aaaatcattt taaatagtgt tgattcattt tatgaactat ctgatttatg tcttacaggg
     2221 ggaagactaa atacgaataa tgtcgtacga caagtaacgg gtaaacttac tgcatctaat
     2281 atcacattaa gattaaatag tgaatggtca gatgaaattg ctaaagagat gtgtcacgtt
     2341 cactggattg accagtcagg aaatgatttg acggatcaag tggttgttct aaaaaacgaa
     2401 gtggatacat ctaactttgg cgattacgaa gtagtttttg catcacctga tttaacagat
     2461 caaatctcag ttacagtgac cgtacctcga ttacgtaaac ttacgctagg aaaagaaaca
     2521 gcaaccattg atattggttc cgcatgggat agtgtcatag ccattgatac ttttcaagtg
     2581 aaagctatag atgattttgg gaatgatgtt acaaatcaag tacaggttac tattaatgct
     2641 gatatagata caattaattc aattcaagca gtgattaagt ttgaagttcc agaattaggc
     2701 ttagcccaaa ttggtagact gaatattact gcaaaaaata gtacaatcaa tggattgaat
     2761 gaagttagca tcgag
//
LOCUS       DACEJR010000153         2757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEJR010000153 DACEJR010000000
VERSION     DACEJR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.163"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(14..706)
                     /locus_tag="IW651_002915"
     CDS             complement(14..706)
                     /locus_tag="IW651_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0815565.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IW651_002916"
                     /pseudo
     CDS             791..967
                     /locus_tag="IW651_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IW651_002917"
     CDS             complement(1143..1712)
                     /locus_tag="IW651_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0815566.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IW651_002918"
     CDS             complement(1786..2109)
                     /locus_tag="IW651_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815567.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..>2757)
                     /locus_tag="IW651_002919"
     CDS             complement(2263..>2757)
                     /locus_tag="IW651_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815568.1"
                     /translation="RSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFP
                     HFFADDGEQSFSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEK
                     NYLAILRLSCNQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKV
                     LYQE"
BASE COUNT          749 a          560 c          488 g          960 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacg
//
LOCUS       DACEJR010000154         2707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEJR010000154 DACEJR010000000
VERSION     DACEJR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.164"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            242..571
                     /locus_tag="IW651_002920"
     CDS             242..571
                     /locus_tag="IW651_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815569.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(858..1343)
                     /gene="vanZ"
                     /locus_tag="IW651_002921"
     CDS             complement(858..1343)
                     /gene="vanZ"
                     /locus_tag="IW651_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0815570.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1496..2407)
                     /locus_tag="IW651_002922"
     CDS             complement(1496..2407)
                     /locus_tag="IW651_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0815571.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          883 a          486 c          410 g          928 t
ORIGIN      
        1 gtagaaatac aaaataataa aactccttta gaacaatttt caaaggagtt ttatttgatt
       61 aggaaattat gtagaataat aatatgtttt taacttggaa tcaaaaacgc acttgcaagt
      121 aagtttactt caatacatga aattgcttat aatagtgtat tggaattaag gtagattgac
      181 aaaagagtga tcaaataggt atacttcatt caaataatcg tttgaatgtg aggtgagatg
      241 aatgaaaaaa gtttgccaag tgactgtcgt acataaagaa aaagtaaatg gaataaaaaa
      301 taaattggat caaaaagatt tttcaaactt acttgtgtta gggaaatgtt tcagcgattt
      361 ttctagaatt aaaattttct atgctttaga aacctataag gaaatgtgcg tctgtgattt
      421 agcagaaata cttactgcta gtgtcgctac aacgtcacac catttacgtt ttttaaaaaa
      481 acatggaatg gcaaaatcac gtcaagatgg aaaagtagtt tattactctt tagcgaatga
      541 agacatcctt tccgctattc gtgtttttta aagttatcag aaaatctatc tatgaaatct
      601 tagtttgggg gtaccgtcag gaaaatgcgg atttacaacg ttaaggaaat ttattaaaga
      661 gaccaattcc tcagcactaa aattgataat tgggctttct ttgttactat agtgttaggt
      721 aagcttttta ctttatcacc ggttttcccc tcacttcaca cctacgggcg agtttcccca
      781 catcccgttt tccatctaat ccaattcatt caatatcttt atgtgtatga aagatcagat
      841 tgcttgattt agacatctta cacgtaattt attcttaaat gggtacggta aacgagcaat
      901 aatacgagca aaagtatacc tacaaaaata ataactctgt ctaatttttt ttgattcata
      961 tgcttattgc ttaaaccata taatttcagt ccaagaaagc ctccaacagt atttgtaatt
     1021 acatctgtta tgtctgtcgc tccaatagcg aagataaatt gaattatttc aaaagtaaga
     1081 cttaaaacca gtacaaaagc aaacttaggt aaaaatccga tttctttaaa attgacattc
     1141 aaaagcaagc caaatggaat aaagattata acattatcta tcatctctct gaaattccca
     1201 gtagcagtaa atggagtcaa gttaagactt ctttgatgat aattaaatac tgataaaata
     1261 ttgtattgta atttgaataa cactaaccag attagtgtca ctaaatataa agctagcaat
     1321 cctctagata atatttttcc caatataatt tcctctaatt cttttgttta agttgtatca
     1381 ttagtatagc aatgtcctag ttaacgtggg ttttaaatta aaaagctaac gttaccccaa
     1441 aagtgctagt acaataatgc tgttcattaa tttcgttggt ttcattccgc catccttacc
     1501 tccttgaatt agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt
     1561 ttcctgatat ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag
     1621 gataataaaa gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt
     1681 ttaggtaatc catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg
     1741 gtaaaccaac atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt
     1801 cctctggata acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt
     1861 caggggctcg ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat
     1921 gctcactata acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt
     1981 gctcatcaaa gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag
     2041 catcattgac catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat
     2101 caagcaaccc gtatccattt attaattcgt catgtttaga taaattcacg atatctgact
     2161 tcacactttc ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa
     2221 cctgttcttc tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat
     2281 gttctttggg attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt
     2341 cattaacgta gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact
     2401 tcttcatttt cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc
     2461 ttccccttaa acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt
     2521 caattatttc gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg
     2581 atacccatgc tgaatttaac gaggctgaac tgcctgttga caatgggcgg tgtgaaatac
     2641 cacccctcta tgcgatcagt cttattatca ggctatcttg ccgcagcgca cgcagataat
     2701 gaccgca
//
LOCUS       DACEJR010000155         2650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEJR010000155 DACEJR010000000
VERSION     DACEJR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.165"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..197
                     /locus_tag="IW651_002923"
     CDS             36..197
                     /locus_tag="IW651_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815572.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IW651_002924"
     CDS             210..713
                     /locus_tag="IW651_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0815573.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IW651_002925"
     CDS             773..1162
                     /locus_tag="IW651_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0815574.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IW651_002926"
     CDS             1149..>2650
                     /locus_tag="IW651_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0815575.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACEJR010000156         2621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEJR010000156 DACEJR010000000
VERSION     DACEJR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.166"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW651_002927"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW651_002927"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0815576.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          939 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg g
//
LOCUS       DACEJR010000157         2557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEJR010000157 DACEJR010000000
VERSION     DACEJR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.167"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            47..298
                     /locus_tag="IW651_002928"
     CDS             47..298
                     /locus_tag="IW651_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0815577.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            400..2397
                     /gene="tkt"
                     /locus_tag="IW651_002929"
     CDS             400..2397
                     /gene="tkt"
                     /locus_tag="IW651_002929"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0815578.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          852 a          467 c          578 g          660 t
ORIGIN      
        1 tttcagtttt tatataatat gaaggagagt aaaaggaggg ctgagcatgc tgtcacctga
       61 aaaaatagag cggatcaatg aacttgcacg taaaaagaag gaaaatggtc tctcaaaagc
      121 cgaagaacaa gaacaaatca acttgcgcaa agagtatttg gaagcttttc gttccggtat
      181 gcgtcatcat atcgaaggaa tgaaagtcgt cgatcctgag ggcaatgacg tgaccccgga
      241 aaaattaaaa gaaatccaaa agaaaaaagg cttacacaac cgaaatgaca acttttagta
      301 aaaaatactg aaaaagattg ctttttcgtg tgacatctta tagaatgaaa gtgtaagaaa
      361 taaaggtaaa aaaaacctaa attaatagga ggggttattt tgtttgacaa aattgaccaa
      421 cttggcgtta acacaattcg tacattaagt attgaagcag tccaaaaagc aaactcagga
      481 catcctggat tgccgatggg cgcagcacct atggcatatg cgttatggac aaaacacctg
      541 aaagtaaatc caacgacatc aaaaaactgg gctgaccgcg accgtttcgt tctttcggca
      601 ggacatgggt cagcaatgtt gtatagcttg cttcatttag ctggctatca agtgacaatc
      661 gatgatttga aacaattccg tcaatgggat tctaaaacac caggacatcc tgaagtacat
      721 catacagatg gtgtagaagc cactacaggg ccattaggac aagggattgc aatggctgtc
      781 ggtatggcaa tggcagaagc gcaccttgca gcaacgtata acaaagaaaa ctttaatgtg
      841 atggatcact atacgtatgc aatatgcggt gacggagact tgatggaagg ggtatctcaa
      901 gaagcaagtt cgatggcagg tcatatgaaa ctagggaaat tgatcgtttt atacgattca
      961 aacgatattt cactagacgg tccaacttca aaagctttca cagaaaacgt tggtgctcgc
     1021 tatgaagcat atggctggca acatatctta gtgaaagacg gaaatgacct agaagcaatc
     1081 tctaaagcga ttgaagaagc aaaagctgaa acagacaaac caacattgat cgaagtaaaa
     1141 acagtcatcg gctatggtgc tcctaaagaa ggaacatctg ctgttcatgg tgctcctctt
     1201 ggtgaagatg gcatcaagat ggctaaagaa gtttatggtt gggagtatcc agatttcact
     1261 gttccagaag aagtagctgc ccgcttccat caaacgatga tagaagaagg acaaaaagct
     1321 gaagatgcat ggaatgaaat gtttgccaat tataaaaaag cttatcctga attagcacaa
     1381 caatttgaag atgcttttga tggcaaacta ccagaaaact gggatgcaga attgccaact
     1441 tatgaagttg gaagcagtca agcaagccgt gtttcaagta aagaagtcat ccaagaatta
     1501 tcgaaagcta taccaagctt ctggggtggt tcagcggact tgtcaggttc caataataca
     1561 atggtggctg cagataaaga tttcactcct gaacattatg aaggacgcaa catctggttc
     1621 ggtgtccgcg aatttgcaat ggcttcggct atgaatggta tccaacttca tggcggaact
     1681 cgtatctacg gtggaacatt ctttgtcttt gtcgattatc taagaccagc agttcgttta
     1741 gcagcaatcc aacatactcc tgttgtttac gtcttaacac atgactcggt tgctgtcgga
     1801 gaagacggac caacgcacga accgattgaa caactagcat ctgttcgctg catgcctggt
     1861 gttcaagtga ttcgtccggc agacggaaat gaaacacgtg ctgcttggaa agtagcgatg
     1921 gagacaacag atgcgcctac tatccttgta ttgagtcgtc aaaaccttcc agtattgcct
     1981 tctacaaaag aagtagcaga tgaaatggtg aaaaaaggcg cgtatgttct ttctccatca
     2041 caaggagaaa caccagaagg tattttgatt gcaacaggtt ctgaagttga tttggctgtg
     2101 aaagctcaaa aagaattggc tgaaaaaggc aaagatgttt ctgttgtttc tatgccaagc
     2161 tttgacctct ttgaaaaaca atcttctgaa tataaagaaa gtgtacttcc taaatcagtg
     2221 aagaaacgtg tggctatcga agctgctgca tcatttggat gggaacgcta cgtaggaaca
     2281 gagggcgcaa cgattacaat cgatcatttc ggtgcatctg cccctggcac taaaatttta
     2341 gaagaatttg gatttacagt agaaaatgtc gtgaatacgt acaatcaact atcatagatc
     2401 caagcaattg tttgactgat aagaacagct gtctgcaagt tggagacagc tgttctttat
     2461 gtgttagaag aataaatcaa tattactttt cgttttcggc tatgttacat ttaaatgaca
     2521 aaacaaagaa ttcttcggat tagtttgtgt ttcttga
//
LOCUS       DACEJR010000158         2522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEJR010000158 DACEJR010000000
VERSION     DACEJR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.168"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(187..552)
                     /locus_tag="IW651_002930"
     CDS             complement(187..552)
                     /locus_tag="IW651_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815579.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            760..1242
                     /locus_tag="IW651_002931"
     CDS             760..1242
                     /locus_tag="IW651_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815580.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1439..2329
                     /gene="pstS"
                     /locus_tag="IW651_002932"
     CDS             1439..2329
                     /gene="pstS"
                     /locus_tag="IW651_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0815581.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          875 a          408 c          498 g          741 t
ORIGIN      
        1 acctcaaatt caatatctat tattggaatc attattgctg caattattac acaactgttt
       61 ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga
      121 tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta catatatggt
      181 ttgtttttat ggatgccgag cttttttata agataaggct accgcccata acgacccaag
      241 gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg gttttaaagg
      301 atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag tgacagtaga
      361 tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat aatatccagc
      421 aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac gaaaatcaag
      481 cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt ttttccagca
      541 agtttttatc atctttcatc accacctaac aagtgttatt attttgttag ttttattgta
      601 aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat gaataaactc
      661 tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat agaaaattgt
      721 atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa agcagtaatc
      781 gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt tacatgggta
      841 aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg atttgtggtt
      901 tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg tctcttatta
      961 ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg cttattagta
     1021 tttacatcta tgttttcttt gctgcctcgc ttgattatcg gccctaatgg tttgagcgag
     1081 agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa accatggttc
     1141 tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac agaaatcagg
     1201 ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat catcagtaaa
     1261 aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt attatgtaaa
     1321 gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta cagcaaccat
     1381 acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga gcaataaaat
     1441 gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat gcggctcagg
     1501 atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa atgaacaagt
     1561 aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag cacaagaaag
     1621 ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa gcggaactgg
     1681 tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat ttgcagaaga
     1741 aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg tcgggatggc
     1801 accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat tgattgatat
     1861 ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa aaatcaacgt
     1921 cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg gactggatgg
     1981 tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc agctggtaag
     2041 tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt ccacacaagc
     2101 actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca gctggggaat
     2161 ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc aaaaattctt
     2221 agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg gctatttacc
     2281 aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac tagcagataa
     2341 acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc acatatacat
     2401 ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag ctaataccga
     2461 gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag aactgtcgta
     2521 ac
//
LOCUS       DACEJR010000159         2515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEJR010000159 DACEJR010000000
VERSION     DACEJR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.169"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(493..1785)
                     /locus_tag="IW651_002933"
     CDS             complement(493..1785)
                     /locus_tag="IW651_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0815582.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IW651_002934"
     CDS             complement(2059..2319)
                     /locus_tag="IW651_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815583.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          847 a          463 c          380 g          825 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagtgt aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aaggc
//
LOCUS       DACEJR010000160         2494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEJR010000160 DACEJR010000000
VERSION     DACEJR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.170"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            282..464
                     /locus_tag="IW651_002935"
     CDS             282..464
                     /locus_tag="IW651_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815584.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            448..633
                     /locus_tag="IW651_002936"
     CDS             448..633
                     /locus_tag="IW651_002936"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0815585.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            655..780
                     /locus_tag="IW651_002937"
     CDS             655..780
                     /locus_tag="IW651_002937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0815586.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            876..2396
                     /locus_tag="IW651_002938"
     CDS             876..2396
                     /locus_tag="IW651_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0815587.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          861 a          420 c          498 g          715 t
ORIGIN      
        1 ttattgaaag attcgattaa attagttgag taaatgcttc gtacgaacat agttgattta
       61 ttcaccatac ttcccattac atcgtaaaac gctatattta agatttcaaa caactatatt
      121 aataggaaga aaacttcatt tttcctctta gttctataaa aagtgtggct aatttgaggc
      181 aaaatattaa attaaatgta agcgtgtcat aggatagtat ctcgtacgcc gattatcttt
      241 cttctataat taaactatca aatatagaag gagaaaaacg aatgaataaa aaagatattg
      301 taccagttca attttcaagt gcggctcaaa aatcgcaaat tgacaccaag ccaaaactag
      361 cttgcagaat caaatcggaa gataaggaaa tatttattta taatggaatg gataaatatc
      421 ttttgtatac cttgttaaaa gagttgaatg ccaatgctca ttgatgcaac tgcattgaaa
      481 catattttta tcgtttgtgg aaaaacagat cttcgaaaag gaattgatgg actagcagct
      541 ttcgttatag aagaatatga ccttgatgtt tacgatcaag cgcttttctt attttgcggt
      601 acgaagaaag atcgcttcaa ggctctttac taggatcaaa atggatttgt attaatgtac
      661 aaaagatttg aaagtgggta ccttcaatgg ccgcgttcca gaaaagaagt cttactgact
      721 ggcctttcaa tcgaagaaaa atgtacgcta aaagaaacga aaaaaggtgc gatatattag
      781 acgaaaacct tgttaaatca aggattttgt ctgtttttcg ttgtcataaa atggtataat
      841 agatgtaaga aaatcgctag aatggtggtg taaaaatgac aaaaatggaa tctgaacttc
      901 ttgtacaaat caagcaatta acacagttgc ttgaaatcca gcaacaggaa aatactcttc
      961 ttagagaaca aattgctgaa atgaatcgac gcttgtttgg acgaaaaaaa gaaacaccac
     1021 ccgtagatgg gcaaatcgat ttattagacg actcaacttt taatgaacca gagcacactg
     1081 gacaagaaag ccaggaaccc attacggtat cttcttttaa acgcagaaaa cgcaagggac
     1141 tcaaagcact ttcattagaa ggtctgcctg aagttcctat tcattatgaa ttaaaaggtg
     1201 ccgattgttt gtgtgaagac tgtggtcata ctttacatga tatcggtgcg acaaggctta
     1261 gagaagagcc attgtatgtt cctgcccata tagaaaaaca agtcatctac caacatgcct
     1321 atcattgcag agaatgtgaa gcattaggag aaacaaaaat caaaaaagcg cctgttccta
     1381 aaccgttgat caataatagt ctaggttcct cttcaattgt gacacagagt atttgggaaa
     1441 aatttgttaa aaaggtgccg gcatatcgac aagaaaaaga atggcatgca cttggtttcc
     1501 ctcttgatcg taaaaaaatc actaattggc atatcaaggt aagtgaaatg gttttagagc
     1561 caatctacaa ttgccttcat caagaattaa gcaatcagga ggtcttacac gcggatgaga
     1621 caagctatcg ggtattgaat actcataaag aaaagacctt ttactggtta tatgcttcag
     1681 gtaaaagcga agaaaaacaa gttgtgctct acgagcacgc aaattcgcgt tccgttgatg
     1741 agccaaaaag ttttttagaa tcatttagcg gtttcttgca tactgatggt tacgcggcct
     1801 atggaaagct gccggtaaac aatgtttatt gttgggcgca tgttcgcaga aagttttttg
     1861 aagcattggg taaacaaccg tccccaaaaa gtcatgccaa aataggatta gacttctgtg
     1921 acaagatgtt tcgaatcgaa cggaccttta ataagcttag cgcagaagaa aggtttgtag
     1981 cgaggaagga aacgttgcag cctgtgatga ctgaattttt tcattggtgt gagagttttg
     2041 cttatttgcc aaaatcgcag cttggaaagg cggttcaata cgcagtcaat cataaagacg
     2101 gacttcagat tgtgttgctt gatggacgct tggaactttc aaataaccga gcagagcgtg
     2161 ccatcaaaga gttagtgatc ggtcgaaaaa actggctatt ttctaaaagc ttaaaaggcg
     2221 ctagatcaaa tggaattata ttgagtatta tccagacagc tgttgcgaat gggttaaaca
     2281 tccgcaaata tttaaatcat ctttttacag agattcccaa tctgtcttcg atgacacctg
     2341 aagcgctacg cgcttatttg ccatggaatc agcagataca agaaatctgt aaataatata
     2401 agccacaaat aacctgattt gagtgtatca aatcgggcta tttgtgttta ggtaccattc
     2461 tatgacacgc ttacgctcaa aactactgaa ttat
//
LOCUS       DACEJR010000161         2389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEJR010000161 DACEJR010000000
VERSION     DACEJR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.171"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            196..2058
                     /locus_tag="IW651_002939"
     CDS             196..2058
                     /locus_tag="IW651_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0815588.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2058..>2389
                     /locus_tag="IW651_002940"
     CDS             2058..>2389
                     /locus_tag="IW651_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0815589.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          859 a          353 c          465 g          712 t
ORIGIN      
        1 aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt taacagtaca
       61 tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta taagttgcac
      121 ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat attagcaatg
      181 taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt gatttacttg
      241 gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa tgttagtgtt
      301 cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg gaagcaagtc
      361 ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga tcgaatcaaa
      421 ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca aagtctccga
      481 atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg aatgaaaatg
      541 gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt gctggaaaat
      601 aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt ttggatcata
      661 ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga gttcacggat
      721 tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga atactttgaa
      781 atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat cattcgaatg
      841 cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt actaaatatt
      901 ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat tggtcgttat
      961 caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt catgcagcag
     1021 gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat ctatagtgat
     1081 acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt gatttataaa
     1141 gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac gaatctgtat
     1201 gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata taatcctttt
     1261 aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca agcagttcga
     1321 caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata tattgcctta
     1381 cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc cgtctacatt
     1441 tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga agaattaaat
     1501 aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat tgctaccaaa
     1561 gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc tttcatcaaa
     1621 gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat aaaaaaaata
     1681 cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac gtccatcaat
     1741 aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac gatttatgaa
     1801 cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc ttttttgctt
     1861 catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc agaaggtcgt
     1921 caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga agcagttttt
     1981 gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc atatataaaa
     2041 gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc gaggaaagtc
     2101 aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta gtagaacatg
     2161 ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat gagatgctga
     2221 aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc aaagaagtat
     2281 ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat ttaaccgatg
     2341 atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACEJR010000162         2270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEJR010000162 DACEJR010000000
VERSION     DACEJR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.172"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1016..1306)
                     /locus_tag="IW651_002941"
     CDS             complement(1016..1306)
                     /locus_tag="IW651_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815590.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2205)
                     /locus_tag="IW651_002942"
                     /pseudo
     CDS             complement(1514..2205)
                     /locus_tag="IW651_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          649 a          432 c          331 g          858 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa
//
LOCUS       DACEJR010000163         2233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEJR010000163 DACEJR010000000
VERSION     DACEJR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.173"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(615..688)
                     /locus_tag="IW651_002943"
     tRNA            complement(615..688)
                     /locus_tag="IW651_002943"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(652..654),aa:Ile,seq:cat)
     gene            complement(877..1896)
                     /locus_tag="IW651_002944"
     CDS             complement(877..1896)
                     /locus_tag="IW651_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0815591.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1907..2113)
                     /locus_tag="IW651_002945"
     CDS             complement(1907..2113)
                     /locus_tag="IW651_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0815592.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          671 a          440 c          378 g          744 t
ORIGIN      
        1 ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg ttatgactat
       61 ataaagtata ctttcgttga tccagaaaga tacaaagatt aaagtaggct acctaaaaag
      121 gtagcccgga acgaatttta tcaccatact tgtgaaagga gatatttttt aagttagtat
      181 taagattgtg taatatgatg atatctatat tttatagtat cagtgctata aaatcaaaaa
      241 taagtcacta attaactacc actccaattg taagcctttt ttctcacttt ttttcaaaaa
      301 tatggtatgc cttttaatag cttcaaatat aaaagagttt aaagcgtaac acacttatgg
      361 ggaagtggct tggggtgcgc tttaaactct tctttattat catctcacaa tttaacccaa
      421 atgtctttct atttaaaaat caaagtaaaa cttttcaaat atacagaaat ataactatgt
      481 gaaacatcct ttcattaatc cataaaagga tacataaaaa agccactcat ttgagtggca
      541 atgaagaaaa gctttagctt gtataatact cttcaaaaaa ttctaacaca gaacgattca
      601 aatggctacg ttaatgtacc ctgtaggact cgaacctacg accggacggt tatgagccgt
      661 ctgctctgac caactgagct aagggtactg gttgttgcca cataaagcca taaacaatca
      721 accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg actattatgg
      781 gtgatagtga agatatgcga taacatcact attttatcga atgattttta tagttgtcaa
      841 tatagttatg taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata
      901 ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt
      961 tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg
     1021 atagccaaca tttccagaag tattataagc ttgatttaca tctgatctat aaattccatc
     1081 agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta
     1141 tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa
     1201 ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg
     1261 tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg
     1321 agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg
     1381 atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg
     1441 gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa
     1501 ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc
     1561 aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg
     1621 ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt
     1681 ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc
     1741 atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt
     1801 aaattcattg ttgatagtat aggcaaatcc gttaattggc aataaaaaaa gagccgttaa
     1861 caggctcatc gcagtaatag taattttctt tttcatatct atcctcctat ttatctgatg
     1921 aaattttctt atacgttcga tttgatacac ctaaaatact ccctagaaat gctccaaaac
     1981 ctgtaataat catcaccgtt atatctgtgt accgccagtt aacacctttt ccgacaagac
     2041 cgacaaaagt cgctaaagcg gggataacta ctatagcgat ccatttcaaa atatcaaagg
     2101 ttctattgtt catactatcc acctcccttc attggttggg ctactttctt tttcaatcgc
     2161 ttgttttctt ctatcaatat ttctctttct gaaagcaagg catcaatttg cccgtccagt
     2221 tgttcaattt tat
//
LOCUS       DACEJR010000164         2197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEJR010000164 DACEJR010000000
VERSION     DACEJR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.174"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..2144)
                     /locus_tag="IW651_002946"
     CDS             complement(147..2144)
                     /locus_tag="IW651_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0815593.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          564 a          493 c          399 g          741 t
ORIGIN      
        1 tttgtcacaa ccttttttgc cgagtaaacg gtgttcaaaa gccagtttct cggtatgaag
       61 tcaaatttaa gcaaaaatgt gagaagctat tttcacaaaa tttttcttca tactcgaaac
      121 taagcggctt tttcacgacc tcttttttat tttgccatga acatatcata atacgcgtcc
      181 attatttctt tagcaatcgt caagtttatg tggtcttgtt cgtctaataa gttcggcaag
      241 acaacactga tggcaatttc cggattgtct gttggtccgt atgccacgat attactattg
      301 actgtcgcaa tatctggatg accttccgca accgtttctg cagtccctgt ttttgcggaa
      361 aggtccattt tcgcactggc taatggtttg gctgttgtgt aaggatcggt accatggaca
      421 acttggtgga atccttcacg gaggatcgcc atattttctg cagaaatatt gaccgtattt
      481 tctactgttg tgccaatttc tttcttcact tcacccaagt ttccattttc gtcattgcca
      541 tagatcgctt tgactaggtg cggctgtact cgtttcccgc cattagcgac tgttgaagca
      601 tattgagcta attggattgg tgtatatgtg tcaaactgtc caaatgccaa atcggtaaag
      661 ttacctggag cataccattc tttgccatta tccgcattga acttcttcat ataatcaaca
      721 gaacgtgaga tcccaggaga ttcattcggc aaatcgatcc cagtcgttgt accaagacca
      781 aactctttga aagagtctct caattgctga taagctttag cttcttcatc taaactaggt
      841 agtcccattc caggtgtata gtctagtcct aacatcttta acgccacttt gatcatatac
      901 gtattagaag acaattccga tgctttgact gcatcaagag caacttgtcc gcttcgatta
      961 aagacagagc ttttttctga agcaccttgc agacgaatcg gctcatcaat caaaacttga
     1021 tttccgctga tcgcgttact tgcccagcct gctgtcaaag taccagcctt gactacagac
     1081 cctggatcaa aggcgctagt aatcgtgcct aaagcattct cggtgatttc tttggaacct
     1141 tgttcatgac tgaaaccagt cattgctagc acttctcctg tctgaggatt catagccact
     1201 gcataagcac caggcgaata ctgtgctttg ccgttgttga tcaatgtttg ataattcctt
     1261 ttcaaaattt cttctacttt tgattggaat tctgcattca tagataacat taaattagaa
     1321 cctttttccc cagagaagat ttctttttga ttcgaaacat ttccttcatt atcaagggaa
     1381 acttcatatt gggttttggt tccttgcagg acatcttcat attgtttttc caaataactt
     1441 tgtcctacac ggtcattccg agaataacct tttgctaggt atttctctgc ttcatctgct
     1501 ggaagtcctt gcttttcagt cgtcacactg ccaagaatac ttttcagtga acttgctgca
     1561 ttatattctc gtgtccagtc tgtgccagtc gaaactcctg gaagttcaga agcgcgttct
     1621 gccacaacag ctaactcttg atcagtaaca ccagagtttt tgatatagac agtgtttaat
     1681 tcatatgcac tattcatccg tttaaagatg gtagcaattt ttaattgctc ctcgtttaaa
     1741 ttaatctcat cagctgtgac tttattgacc atcgcactat attgttcgct gttccctaat
     1801 tgcttttctt ttgccgaaag tctttttgtg gcttctttca aatgtttggg atcagccagc
     1861 caaaaatctt ttttatctct atctgtcaga ttttcatcta ccggaacatc gatcagcgag
     1921 ttcagcttcg ttgcgatttc aagcaaatct ttagccgtaa cttgtgttcc tctcgtatat
     1981 gtgatggcct gattcgcttt attcgtaact aaagcttttc ctgtggagtc gtagatcatt
     2041 ccgcgaggag tactttcttg gaccgtaatc gtagaagtag acttcactcg cgcctccatc
     2101 tcttcgccgt tcacgatctg caaataaccc aatctagcaa tcaacgcaac aaataacgta
     2161 aatataatga aaaaaagaaa atttaagcga aaaggaa
//
LOCUS       DACEJR010000165         2080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEJR010000165 DACEJR010000000
VERSION     DACEJR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.175"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            848..1321
                     /locus_tag="IW651_002947"
     CDS             848..1321
                     /locus_tag="IW651_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0815594.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1330..1557
                     /locus_tag="IW651_002948"
     CDS             1330..1557
                     /locus_tag="IW651_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815595.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          677 a          333 c          313 g          757 t
ORIGIN      
        1 tactattctt ataaataaaa acgaggtatg tcctatgatg aaaagattgt actattcatt
       61 gatcataacg attggctatt tgattgtatc aaatctagga aatatggtat ttggtatcag
      121 taaagaattc agttggacta ctacattatg ggaatctcta ttctttttca tcttcgtatt
      181 tttgttacaa aattatcgaa aaaaataaac atgtgccatc tatctgttgg cacatgtttt
      241 aggatttatg gtacttttta ttcggcaatc cattttattt ttctacttct tcttaatacc
      301 tctaacactt ttgatttata aactaatcca atgcatctac ttgcgcttat taagctattt
      361 cagctagata gtaccacccc atgcgtcaat gcttgatttt ccttttgtat aacattgtat
      421 aagcaataaa ggacaaataa gtggtgatta caccaaacca ttgaataaga aaaattctga
      481 tacgtaaata aacatgaact tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc
      541 ttttttctca tacagttctg ttaacttaca cttctcttcg ctagataata aaaaatcctc
      601 caactgtatt gtccaatata atcccaggca tctaaactag cattatagaa gagacacaca
      661 tagaatctaa aataactggt tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca
      721 ctccacacct tcatactcta tcatttttta taaacgatat atttttattg tttttcgata
      781 aaaaatagtt gttttacaat aattattctg ctatatttta gctatcaaag ataaaagagg
      841 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
      901 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
      961 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
     1021 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
     1081 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
     1141 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
     1201 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
     1261 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
     1321 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
     1381 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
     1441 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
     1501 caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga agaataaaat
     1561 aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga gacttttaga
     1621 agttatttac gtctattatt cagatttttt atttcgattg aataccgttt tatctataaa
     1681 taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact atcacttttc
     1741 ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat aatactttat
     1801 tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt attacaggcc
     1861 attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg tgttaactct
     1921 tactccattc ttttttagta agctcccata tatactgtaa agccttctat atattatgtc
     1981 atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata acagtgatgg
     2041 acaattcttt tttattttta acattatttt gataataatg
//
LOCUS       DACEJR010000166         2054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEJR010000166 DACEJR010000000
VERSION     DACEJR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.176"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            63..392
                     /locus_tag="IW651_002949"
     CDS             63..392
                     /locus_tag="IW651_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0815596.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            382..669
                     /locus_tag="IW651_002950"
     CDS             382..669
                     /locus_tag="IW651_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0815597.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(948..1511)
                     /locus_tag="IW651_002951"
     CDS             complement(948..1511)
                     /locus_tag="IW651_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815598.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          737 a          310 c          331 g          676 t
ORIGIN      
        1 aaaaatagcg tacaatgtaa ttactaagta attacgaaat gtaaaataat ggaggaaata
       61 atatgattac aaaaacaaga aaacaaggaa attctattat gttaacggtc cctaaagaat
      121 tcgatgtgcc caatggtgtt gaagtagaag ctaagcttgt agagaatggt atcctttatg
      181 agtttgtaga gcctaaaaaa gaattttttg attttagtga agatattttg gctgatattc
      241 tttcagaagg ttatagcaaa caagagatct taaaagaatt tagaaataga aaaaatgcgt
      301 taacttctgc atttaaatct attgctgaag atactgttgc aaattctaaa ccgatgacaa
      361 aagaggagtt ggcagcagaa attggcttat gagattctac cttctaaaca cgtaataaag
      421 tatctaaaaa aattaaaaga gaaaacatta aaagaacaat ttttaacgat aatttatgat
      481 gagatagcag ttcgtcctca ttcgggagaa caaaaaacgg gtgatttatc aggaatttgg
      541 gctattggat tcaagtatgc aggtacgaca tatcgcgttg catatgaaat taaagataat
      601 gcagtgatac ctatcttact atgtggaacg catgaaaatt tttatgaaca attaaaaaag
      661 attaggtaag aacccaatgt ttcaacttga gaggatagac gacactttaa aagataattt
      721 ttaacagctg agagttgtaa taccctataa ttttaaacca ctaacctata tatttatgaa
      781 aaatagaaaa aattaaaacg cattttaagc ctctttcaaa ttaaaagctt catccttatg
      841 cacaaagagg gcttaaaatg cgttatttta tattgttttt tagtaataat aaaaaaatca
      901 ctctaattct cccataaata ttatagattc ctaaagatta aaatagatta ttccctattg
      961 tcttttaagt agcggtagag tgttgccttg ctaataccag tcatttccgt aatttctttt
     1021 acgctataaa cctgactttt atacaatttt actgctcgat caacatcttt ctggttcact
     1081 cgtggacggc cacctttacg acctctcgca cgtgcacttt tcaaaccttc tttggtacgt
     1141 tcaacaatta aatcacgttc gaactgacta aatgcttgga agacagtcat cattaagcgt
     1201 ccgtgaggcg tgctggtatc gaagttttct ttcaagctca ccagcttaac atggtgttct
     1261 tcaaaatagg taacaaggtc aatcaaatcc tttgtactac gacccaatcg agaaaaactt
     1321 tctacaatta ccgtatctcc cggacgaatt ttatctttta aacgatttag ttggggacga
     1381 accgctttgg ttcccgtcat tttctcagtt aggatttcat tacaattttg ttcattgagt
     1441 aagtctaatt gacgatttag ttcttgtgaa cgtgtactca ctcgggcata accataaatg
     1501 tatccactca tgcttttctt cctttcgtac tttaattctg gcttgattgt atcataaaag
     1561 ggagttatag atacatagtt aatgcaacaa gttatgatac aaaatatgac ctcttttatt
     1621 taataaaaga ggtcatttca ttttgtctca taatcgattg tttttgatac gagtgtgtat
     1681 acgaaataaa caaaaaaatc ttaagtaaat ttctagatac ctaatttaat cctaactagt
     1741 gtatcctaaa gttaataacg agtttatcac aatgaaaatt ataaaatgct cccatattag
     1801 atataacatg caatatttga attagaacta caaaatttat tcgttttata aagtaaataa
     1861 tgttgagata ctaaggaata gaatattata aggaattgag ctgaatgtat tggataaaaa
     1921 aagaataact ttattagaga tggactataa aaatgctcgt aattttcttc tgaagtctga
     1981 aagctatttg aatgctcagc taccagaata tattagtttt gacgaaactt taaaatctgc
     2041 taaaaaagtt ttaa
//
LOCUS       DACEJR010000167         1960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEJR010000167 DACEJR010000000
VERSION     DACEJR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.177"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..424)
                     /locus_tag="IW651_002952"
     CDS             complement(<1..424)
                     /locus_tag="IW651_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0815599.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IW651_002953"
     CDS             complement(533..1564)
                     /locus_tag="IW651_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0815600.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          604 a          325 c          338 g          693 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac
//
LOCUS       DACEJR010000168         1701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEJR010000168 DACEJR010000000
VERSION     DACEJR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.178"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..255
                     /locus_tag="IW651_002954"
     CDS             <1..255
                     /locus_tag="IW651_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815601.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="IW651_002955"
                     /pseudo
     CDS             258..411
                     /locus_tag="IW651_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="IW651_002956"
     CDS             434..1639
                     /locus_tag="IW651_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0815602.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DACEJR010000169         1700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEJR010000169 DACEJR010000000
VERSION     DACEJR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.179"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            466..1065
                     /locus_tag="IW651_002957"
     CDS             466..1065
                     /locus_tag="IW651_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0815603.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1700
                     /locus_tag="IW651_002958"
     CDS             1129..>1700
                     /locus_tag="IW651_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815604.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          612 a          257 c          321 g          510 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga
//
LOCUS       DACEJR010000170         1682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEJR010000170 DACEJR010000000
VERSION     DACEJR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.180"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..742
                     /locus_tag="IW651_002959"
     CDS             200..742
                     /locus_tag="IW651_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815605.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IW651_002960"
     CDS             898..1347
                     /locus_tag="IW651_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0815606.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1377..>1682)
                     /locus_tag="IW651_002961"
     CDS             complement(1377..>1682)
                     /locus_tag="IW651_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0815607.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          635 a          241 c          323 g          483 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gt
//
LOCUS       DACEJR010000171         1635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEJR010000171 DACEJR010000000
VERSION     DACEJR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.181"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            519..1073
                     /locus_tag="IW651_002962"
     CDS             519..1073
                     /locus_tag="IW651_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0815608.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1327..>1635
                     /locus_tag="IW651_002963"
     CDS             1327..>1635
                     /locus_tag="IW651_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815609.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          656 a          172 c          277 g          530 t
ORIGIN      
        1 agcaaggaaa gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat
       61 cttattatct ttagtgatat aacgaaatat ctacacagac aaaattatga tcttgcgtaa
      121 agttataaaa tattaataaa aatattttat taatgaaaag cttggtagca cggttattca
      181 tatgctaatt tatttcactt tatagtaagg ggagttctta gtatattgtc caaatttaaa
      241 tttcatataa tttaattatg taaattagct attctaagaa cttctacgga taactgcccg
      301 tctaaaattt aattaagagc ttagaatctt catataagta aggttaattt ttttgtacaa
      361 aatagtcgga atattctgag ttactgaaat gatataaaat cataaactaa accccttgat
      421 tttcttcact tttaacagag tatatatata agttcatgat tatggtaaaa tatatatata
      481 atcatgaact cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt
      541 taaggacaaa tgaagagata caagattttc agttttggct aagaagaaca agtaatccag
      601 aaagagatag ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta
      661 taggattaaa agttggagac atacgaggta aaagtaagcc tattattgtt gaacataaaa
      721 caggtaagcg aaaaccgatt tttatagata atttgagaga agagatttta ttatatactg
      781 aaggtaaaga agaaaatgat tggttatttc caagtcggca acaaggtcga catataacaa
      841 gggatcgtgt ctatcagata tatgcagata tagcagaaaa attaggaaga gatgatatag
      901 gaacacatac attaagaaaa acttttgggt atcactacta caagaaaact agagacattg
      961 ctaccttaat gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta
     1021 taactgaaga tgaaataggg gcatcgttac gtggttttaa attgggaggc taattgagat
     1081 attaggatat attttttgtg tataatgaaa acttagaagg tgagattaat gaagataact
     1141 gaattttcgg aagtatttaa aacgtttagt aacatttaca atagaatacc agagatagaa
     1201 caaaatgttg tagttgataa tgattattat atagagaatg attttaataa accaagaaaa
     1261 tatgacaacc tatacaattt tatacacaat agaacaacaa taaataacaa cagtaacact
     1321 cgagttttga ttaattcagc atattttcat attaaatatt taaagaaata tgagtatgaa
     1381 gaagttgtat tagattatgg gctaaataga cagtcatttt taatttatgc aataaataac
     1441 ggaaatttgc caagcaataa aaagaacata attattgaaa catatgccta tctggagggg
     1501 ggatctaaaa aactaattaa gtcatgtaat atagagtatc aattatcagt aggagaaatt
     1561 aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa aaatttttgc cagcattcat
     1621 gattatatag gtttg
//
LOCUS       DACEJR010000172         1607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEJR010000172 DACEJR010000000
VERSION     DACEJR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.182"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..280)
                     /locus_tag="IW651_002964"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IW651_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IW651_002965"
     CDS             complement(546..740)
                     /locus_tag="IW651_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815610.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          567 a          242 c          213 g          585 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaata
//
LOCUS       DACEJR010000173         1594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEJR010000173 DACEJR010000000
VERSION     DACEJR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.183"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1485
                     /locus_tag="IW651_002966"
     CDS             <1..1485
                     /locus_tag="IW651_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0815611.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DACEJR010000174         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEJR010000174 DACEJR010000000
VERSION     DACEJR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.184"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW651_002967"
     CDS             complement(150..1337)
                     /locus_tag="IW651_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0815612.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEJR010000175         1409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEJR010000175 DACEJR010000000
VERSION     DACEJR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.185"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1359
                     /locus_tag="IW651_002968"
     CDS             <1..1359
                     /locus_tag="IW651_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0815613.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DACEJR010000176         1396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEJR010000176 DACEJR010000000
VERSION     DACEJR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.186"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1346
                     /locus_tag="IW651_002969"
     CDS             <1..1346
                     /locus_tag="IW651_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0815614.1"
                     /translation="YDTVANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGA
                     KRTVFFGGVFIMLGHIVLALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDL
                     RRDAGFSIFYMGVNLGALLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKT
                     LDGVGTTPPNPLTTEERKGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGI
                     ILPLYYFIKMLTSKDVTAEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQS
                     TLFGVTIQASWYQSLNPIFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLL
                     LMLPGMLYGTSGKVSPLWLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWF
                     LADASSQAINAQIARFYTPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          380 a          269 c          290 g          457 t
ORIGIN      
        1 tgtacgatac agttgccaac gggggacttg gattaccacg agcaacagca ttagctatca
       61 tgtcgatcta cggatctttg atctacatgt ctagtatcgt cggcggatgg gtatctgacc
      121 gagtgttagg agcaaaacgc actgtattct tcggtggagt tttcattatg cttggccaca
      181 tcgtcttggc tttaccattt ggtgtaagta cattgtttgt ttcgatggca ttgattatta
      241 tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt cggacatctt tattcaaaga
      301 ctgacttgcg aagagatgca ggattttcga ttttttacat gggtgtcaac ttaggggcat
      361 tattagcacc actaatcgtt ggtacaatcg gtcaagaata taactatcat ttagggttct
      421 ctttagctgc tattgggatg ttctttggat tattacaata catcatccaa ggacgcaaga
      481 cattagacgg agtgggtaca acaccgccga atcctttgac aactgaagaa cgtaaaggat
      541 tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc agtattcggc ggggcacaac
      601 taactggtca tctgacaatt gacttttttg tcaatacaat cagtgtattg gggattatct
      661 taccgttgta ttactttatc aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac
      721 caagagtatg ggcttatatc ccattattcc ttgcagcaat cattttctgg tcattacaag
      781 aacaaggttc atctatctta gctttatttg ctagtgaacg tacacaatct acgctatttg
      841 gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc aattttcatc tttattctga
      901 caccagtatt cgtaacgatg tggacgaaac ttggtgaacg tcaaccatct acagtagtga
      961 aattctctct tggactttta tttgcaggat tatcgtactt gctattgatg ttgccgggta
     1021 tgctttatgg tacaagtgga aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg
     1081 tggttattgc tgaattgtgt ctttcaccag ttggtttgtc tgtgacaaca aaattagcac
     1141 cacgagcatt tgaatcacaa acaatcgcta tttggttctt agctgatgct tcttcacaag
     1201 caatcaatgc acaaattgca cgcttctaca caccgggaac agaatctgct tacttcggta
     1261 ttatcggtgc agtagcaatc gtcggtggta tcttgctatt tactgtgaaa gaaccaatca
     1321 aaaaactaat gggctctatt cattaatcaa tgaacataaa aacaggaatt gcttatttgc
     1381 agcagttcct gttttt
//
LOCUS       DACEJR010000177         1367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEJR010000177 DACEJR010000000
VERSION     DACEJR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.187"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..1365
                     /locus_tag="IW651_002970"
     CDS             join(26..467,467..1365)
                     /locus_tag="IW651_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0815615.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          573 a          176 c          237 g          381 t
ORIGIN      
        1 aatctaatta aaaggggtgc tttttttgcg gaaatataca tttgttttta agaaaaaagt
       61 agtttcagac tatttaaata acgaaggcgg ctacaaatat cttgcacata aatatcaaat
      121 aaatcgtaca ctggttagac attgggtaag gatttataac tatcatggtt gggaaggctt
      181 ggttggaggt ggcaaaagct acactacaaa atttaaactt gatgttatag aatatatgga
      241 aacaaatggt ctttctatcc aagaaactgc taaaaaattt aatatcggtt caaatagaac
      301 tctaagtaaa tggatagagc aatatgaaga aggcggtgct tcttcacttg agagccaaaa
      361 aaggggcaga aaaattagta tgaattccaa gctaaacatt cctaaaaaac ttaaagatga
      421 gtctcttgaa gaagaagtta ttcgtttaag agcagagaac gcatatttaa aaaagttaga
      481 aaccttgatt caagaacaag atttgagcaa gaagaaatca aggttaaaac aatccttcag
      541 ctcaaaaaag aatttaaatt gaatctatta ctttcaattg cacaattagc gaagtcaact
      601 tattattact gggtaaaaaa attagataag ccagataaat atagcaagat aaagcaagaa
      661 attacagcga ttgtaaaaga atcaagaaac tcttatggtt atcgtagagt cactttagcg
      721 ttaaagatga aaggatatac aatcaatcat aaaacagtta gaaaattaat gtcccaaatg
      781 ggacttacct gtcaaatccg aataaagcga tataaatctt ataaaggaac agtaggaaaa
      841 attgccaaga atgtgttaaa acgaaatttt tcagtagata cacccaataa aaaatgggtg
      901 acagacgtca ctgaattcaa gataaaagga agaaaaatct atctatctcc tatacttgat
      961 ttatttaacg gagaaataat tagttatagt atatcaacta gtccaacata taaactgatt
     1021 gaagaaatgc ttcaacaagc aattaaaaaa aagggaactg agggttcact gattctacat
     1081 tcagatcaag gatggcagta tcagatgcca caatatcaaa aaaaattaaa agagaataac
     1141 attattcaaa gcatgtctag aaaaggaaat tgcttagata attctgtaat agaaaatttt
     1201 tttggtgtgc ttaaatcaga gtttttttat cgagaaaaat tccgatcaat tgagatattt
     1261 caaagtaaat taaatgagta tattaggtgg tataataaca aaaggataaa actaaagtta
     1321 aatggactat ctccagtaga atatcggaaa cagtccatta aatagtt
//
LOCUS       DACEJR010000178         1362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEJR010000178 DACEJR010000000
VERSION     DACEJR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.188"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>1362
                     /locus_tag="IW651_002971"
     CDS             82..>1362
                     /locus_tag="IW651_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0815616.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DACEJR010000179         1362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEJR010000179 DACEJR010000000
VERSION     DACEJR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.189"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..794
                     /locus_tag="IW651_002972"
     CDS             51..794
                     /locus_tag="IW651_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815617.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(831..1328)
                     /locus_tag="IW651_002973"
     CDS             complement(831..1328)
                     /locus_tag="IW651_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0815618.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          430 a          239 c          230 g          463 t
ORIGIN      
        1 tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat atagtagtct
       61 caaaactccc aagaaatgag gactatttaa caaactacga tggttacgtc gtgacaactg
      121 aacattttga cttagccaat cctgttacta aagtcgatga tatccccgat tatgaaatgt
      181 acagtcaaac aatcgatagc ttaaataagc gttttggcaa tcgtgtttta aaaggcatca
      241 aaatcggtta catcgcttct gaaaaggatc ggatcatcga ctatttagcc gataaagatt
      301 acgatttaaa attactcagt gtgcatcata atgggcaatt tgattattta gatgacgaag
      361 tcaaagatat ggatcctgct attgtgattc ctcagtattt tgcacaactt tcagaagcac
      421 tagttgttat agaagcagat gtctttgctc attttgacta tggttttcgg gtatttggtc
      481 tatccgttgc tgaatttaaa caatatgaag cgcagttttt accgattcta gatcaagtaa
      541 tcaaaaataa attggcattc gaattaaatg ctaagagcgc ttacttgtat gacaatttag
      601 cgttatatga atatgtcatc gatttatatc tttcacgtgg tggtaccttg tttagtgttg
      661 gatcagatgg gcattacctt gaacatttcc gtttccattt cgatgatctt tttgctctgt
      721 taaaagcaaa tggtgtgacg gaattagcga tttatcaaaa aggaaagcga attatggttc
      781 cattaccagt ctaaaaaaac gtcctcagca tcaaaagtga ggacgttttt ttagatttct
      841 ttttctaaaa tcggacaaat acgatcttgt tttcctaaag gaaaatgttt gatcccgatt
      901 tttttaaatc catgacgttc ataaaaacga atacctgcat gatttttttc ataaacagtc
      961 aaacgtaaat aacgtttttg tttcgtttta gcataacgat tcgcttcatc gagtaaccgt
     1021 tgccctaacc ctttgttttg ataatctttg agcagataga gccgttgaat ctctatagca
     1081 tctttttcat cattgacttt catataacca atgagtattt gatgatcctt aataccaata
     1141 aatttactgg ttgactgtgc caattctact gccaatattt cagttgtcaa ttgtgtctgt
     1201 aaatatcctt gcatatcaag tggatctgcg ccatcgataa acgattcata aaaactgaca
     1261 acacttacaa cttgtaaagc atctagatca tcataatcta aatacgctag tgtgatttga
     1321 ttgttcatct aactcctcct tttgacatag tatactataa at
//
LOCUS       DACEJR010000180         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEJR010000180 DACEJR010000000
VERSION     DACEJR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.190"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(390..>1316)
                     /locus_tag="IW651_002974"
     CDS             complement(390..>1316)
                     /locus_tag="IW651_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ0815619.1"
                     /translation="YESLLVKICEDYYLEDINISEISKKYGITRYKALKYIEDAREKQ
                     LVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADYIQELIKK
                     ANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQKAAERYES
                     SYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDSVEPWNKH
                     RKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGICKSKFKA
                     ESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
BASE COUNT          483 a          208 c          214 g          411 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcata
//
LOCUS       DACEJR010000181         1313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEJR010000181 DACEJR010000000
VERSION     DACEJR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.191"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            96..>1313
                     /locus_tag="IW651_002975"
     CDS             96..>1313
                     /locus_tag="IW651_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0815620.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          429 a          270 c          239 g          375 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gca
//
LOCUS       DACEJR010000182         1307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEJR010000182 DACEJR010000000
VERSION     DACEJR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.192"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            367..1278
                     /locus_tag="IW651_002976"
     CDS             367..1278
                     /locus_tag="IW651_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0815621.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          524 a          195 c          255 g          333 t
ORIGIN      
        1 tgtataaaaa tatatcataa taaaaacaac aaaaaaatgt ctggaaaatc gaacataaaa
       61 gtgtctgatt tgaatatccc agcagcggaa gaagaaaaac aaaatcgata acatttgttc
      121 taagaattag ttacgttaaa tacggaacgc aagatagact tgaaaaacgg taacaaaaga
      181 agtagtatat taaggtgtga tgtgctactt gacatttatt ctgctgagcg aactttctgg
      241 attgtgtagc cttatatcta ttcaggacgt atcgagcaga ttagtaaagg tgttcttgat
      301 gattttcatc ttgaacacct ttgtttttag caattcgcaa aaaacgaaaa gtaggagaat
      361 tgataaatga aaaaaagatt tttagcactt gccatcgtac taggcactgg cttattgtca
      421 ggctgtacga atgccggtga aaaaacagcc gttagttata aaggcggtac aatcagcgaa
      481 caagaagtaa tggatagctt gaaaaaaatg caaggagcag attccgctgt gcaacagttg
      541 atcgtttatc aagtatttga agataaatat ggagatgacg tatcaaccaa agaaatcgat
      601 tctcaatatg atcaaacaaa gaaacaattg ggtgattcat ttgattcaca attgaaatct
      661 gcaggttaca cagaacaaac attcaaagac agtatcaaac aaagtctagc ctttcaagaa
      721 ggtctaaaga aacatatcaa attgaccgat gaagatttaa aaacggcttg ggaatctttc
      781 catccagaag tggaagctca aattatccaa gtagcaagtg aagatgatgc aaaagatgtg
      841 aagaaagctg ctgataaggg agacgatttc tctaaattag ccaaagacaa gtcaactgat
      901 actacgacaa aagaagatgg cggaaaagtg aaatttgatt ccactactac aacggttccg
      961 gcagaagtca aagaagcagc ctttaagttg aaagatggtc aagtttctga tgtgatcact
     1021 tctaccaatg cttcaactta tactacggaa tattatgtgg ttaaaatggt gaaaaaccaa
     1081 aacaaaggca acgacatgga taaatacaaa aaagaattga aagaaattgc aacagacaca
     1141 aaattaagtg acagtacctt ccagaataaa gtaattggcg aagtattgaa agacgctaat
     1201 gtaaaaatca aagacaaaga ctttgaaaat gttttgagta cattcacttc agattctagt
     1261 acagcaagta gtaaataagc ggtataaata aaaaatatga actattt
//
LOCUS       DACEJR010000183         1291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEJR010000183 DACEJR010000000
VERSION     DACEJR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.193"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..>1291
                     /locus_tag="IW651_002977"
     CDS             50..>1291
                     /locus_tag="IW651_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0815622.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVS"
BASE COUNT          507 a          166 c          218 g          400 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc t
//
LOCUS       DACEJR010000184         1287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEJR010000184 DACEJR010000000
VERSION     DACEJR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.194"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            316..570
                     /locus_tag="IW651_002978"
     CDS             316..570
                     /locus_tag="IW651_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0815623.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IW651_002979"
     CDS             571..888
                     /locus_tag="IW651_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0815624.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          449 a          208 c          255 g          375 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatg
//
LOCUS       DACEJR010000185         1230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEJR010000185 DACEJR010000000
VERSION     DACEJR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.195"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..372
                     /locus_tag="IW651_002980"
     CDS             <1..372
                     /locus_tag="IW651_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0815625.1"
                     /translation="ANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMD
                     YNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEG
                     NTKGGAQDFHDKRWYLNIPKR"
     gene            566..639
                     /locus_tag="IW651_002981"
     tRNA            566..639
                     /locus_tag="IW651_002981"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Ile,seq:cat)
BASE COUNT          398 a          213 c          225 g          394 t
ORIGIN      
        1 gcaaatccta cgctgacaga tggaacaaac tacgctcata ttgatcagtt tggggaaatt
       61 gaaaatgcaa acttacacgt cgctggatgg cacatcgcta actataaata cgagtatatt
      121 ttcattatgg actacaatac tggaaaagaa ctagctagag taagagctga tggaatttat
      181 agaccagatg taaatcaagc ttataatact tctggaaatg ttggttatca tgtatctttc
      241 aatatgcgta attttcctag taagaaagtc tatgtcatga tgcgggcaac gaatgatcca
      301 gaaggaaaca ctaaaggcgg tgcgcaagat ttccatgaca aacgttggta tttaaatatt
      361 cctaaacgat aaaaatagct cctcgttgag gagcattaca taactatatt gacaactata
      421 aaaatcattc gataaaatag tgatgttatc gcatatcttc actatcaccc ataaatagtc
      481 acactccaag ctatgcgata acaggtttgt tgccacgatt tatttccaga cgtttgtatt
      541 ttgtatggct tgttgtggca cacctgtacc cttagctcag ttggtcagag cagacggctc
      601 ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat aagataaatt cagtttatta
      661 accgtttgtc gaacacttac ccgattagaa ttttaaatac gtttttgact acgttttgaa
      721 atttagttag tactaatatt ttataactag aagcaaaaat tagggtaaat cgctaaaata
      781 tgctagtaaa tttcttctat taccagtcat atataaatcc tgtaccttcc ttatatcaac
      841 gattagaggt tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata
      901 gcttttccta ttttttcgtt tgattgggct tattaccgca gatgcaagct tttgaacacc
      961 gtttgttcgg aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt
     1021 ttaacctaca atattttttg attccccctg agtactattt tttataaatg ctagtaaatc
     1081 aatcttcttt ggcctttttt tgataaaaaa aatactagat gtaagataaa agtgtaaaaa
     1141 cgatttgttt taggacggga aacgatgtgt accgcaatca cttatgcaac gaaagaccat
     1201 tattttggaa ggaattttga ttatgagatg
//
LOCUS       DACEJR010000186         1206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEJR010000186 DACEJR010000000
VERSION     DACEJR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.196"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1206
                     /locus_tag="IW651_002982"
     rRNA            <1..>1206
                     /locus_tag="IW651_002982"
                     /product="23S ribosomal RNA"
BASE COUNT          314 a          263 c          371 g          258 t
ORIGIN      
        1 taaggaactc ggcaaaatga ccccgtaact tcgggagaag gggtgctgat catacgatca
       61 gccgcagtga ataggcccaa gcgactgttt atcaaaaaca caggtctctg caaaatcgta
      121 agatgaagta taggggctga cgcctgcccg gtgctggaag gttaagagga gtgcttagcg
      181 caagcgaagg tacgaattga agccccagta aacggcggcc gtaactataa cggtcctaag
      241 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatttgggca
      301 ctgtctcaac gagagactcg gtgaaatttt agtacctgtg aagatgcagg ttacccgcga
      361 caggacggaa agaccccatg gagctttact gtagtttgat attgagtgtc tgtaccgcat
      421 gtacaggata ggtaggagcc gtagaaatcg gaacgctagt ttcgatggag gcgctggtgg
      481 gatactaccc ctgcgttatg gccactctaa cccgcaccac taatcgtggt gggagacagt
      541 gtcagatggg cagtttgact ggggcggtcg cctcctaaaa ggtaacggag gcgcccaaag
      601 gttccctcag aatggttgga aatcattcga agagtgtaaa ggcagaaggg agcttgactg
      661 cgagaccaac aagtcgagca gggacgaaag tcgggcttag tgatccggtg gttccgcatg
      721 gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta tctcccccaa
      781 gagtccacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg
      841 tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga
      901 acgtcgtgag acagttcggt ccctatccgt cgcgggcgtt ggaaatttga gaggagctgt
      961 ccttagtacg agaggaccgg gatggactta ccgctggtgt accagttgtt ctgccaaggg
     1021 cattgctggg tagctatgta gggaagggat aaacgctgaa agcatctaag tgtgaagccc
     1081 acctcaagat gagatttccc atttctttaa gaaagtaaga tccctgagag atgatcaggt
     1141 agataggtca ggagtggaag tacagtgatg tatggagcgg actgatacta atcgatcgag
     1201 gactta
//
LOCUS       DACEJR010000187         1187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEJR010000187 DACEJR010000000
VERSION     DACEJR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.197"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..143
                     /locus_tag="IW651_002983"
     CDS             <1..143
                     /locus_tag="IW651_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0815626.1"
                     /translation="QSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKVLMGI
                     TA"
     gene            149..565
                     /locus_tag="IW651_002984"
     CDS             149..565
                     /locus_tag="IW651_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815627.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            671..934
                     /locus_tag="IW651_002985"
     CDS             671..934
                     /locus_tag="IW651_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0815628.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            934..>1187
                     /locus_tag="IW651_002986"
     CDS             934..>1187
                     /locus_tag="IW651_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0815629.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          450 a          153 c          243 g          341 t
ORIGIN      
        1 atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt cgaggaatat
       61 ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact gaaatcaagg
      121 tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt ttgattttca
      181 aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac agttagaaaa
      241 catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat ttgaagtttt
      301 gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga taaaagcaca
      361 ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta gagggaaaat
      421 caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg taatgataac
      481 agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga taaaacttgt
      541 taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact ccaatttaaa
      601 caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat aaattagagg
      661 gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa atttttataa
      721 gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg agcgcagtga
      781 aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa cgctattact
      841 tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata gtggatttac
      901 taatgttgat gaaattgatt gggataactt ataatgaata actattctgt gatgattaaa
      961 aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc tcaatttgag
     1021 aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt tgagaagtta
     1081 aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg ggttgtttat
     1141 aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggac
//
LOCUS       DACEJR010000188         1187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEJR010000188 DACEJR010000000
VERSION     DACEJR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.198"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..771
                     /locus_tag="IW651_002987"
     CDS             <1..771
                     /locus_tag="IW651_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0815630.1"
                     /translation="STREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLV
                     KITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADV
                     EKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTR
                     RLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFK
                     VSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            843..>1187
                     /locus_tag="IW651_002988"
     CDS             843..>1187
                     /locus_tag="IW651_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815631.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          523 a          136 c          196 g          332 t
ORIGIN      
        1 tcgactaggg aaaaaatcat aaaaatattt gataaaatta ataatttatt caagaatgac
       61 aatgctgaat taaggaaggg tcttttagag tttaaacttc aagaactaga acttcattac
      121 tcttatttag tgaaaattac cgaagaaaaa gaacaacaaa aagcaataaa agaacagatg
      181 atagaagagg aaaaagttcg tagagaaatt gatcgtgaaa agaaacgtat tgataaagaa
      241 caacgtcaat tcaattctga aatttccaaa ttaatcgcat atatgcaaaa agctaatgca
      301 gatgtggaaa aagaattata tgcaaataaa attcaagaac tggaagaaaa attaaaagaa
      361 ttagaagtta tacgtgaaaa tgtattacaa agagaattaa atactagagc cggttatgtg
      421 tatgtaatta gtaatattgg gtcttttggt gaagacatat ataaaattgg tatgactaga
      481 agattagaac ctatggatag aataaaagaa ttgagtagtg catcggttcc atttgaattt
      541 gacgttcatg cgatgatttt ttcagaggac gctccgtctt tggaaactaa attgcataat
      601 cattttagaa aacaagaagt taataaaatt aatcaaagaa aagaattttt caaagtttca
      661 ctagacgaaa tagagaaagt tgttttagaa aattataatg gaacagtaac atttacaaaa
      721 ttagctaaag ctgaacaata tagacgatct ttagaattgt caaaagatta aatatataaa
      781 aagccttcgg gctttttctt ttaacaatta ataggaacgt atattcgaaa ggaatagaaa
      841 taatgtggtt agaaaaaata aatgacaaaa aatttaagta caccgaacgt tatacggatc
      901 ccttaactga aaaaaagaga aaagtgtccg ttactttatc tagcaattca cgccaagctt
      961 ggaatcaagc taacttactt ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag
     1021 aattaccact aacttttgga gaattaaaaa ctaaatggga tgaaaaatac aagccaactg
     1081 taaaagaaag ttcctatcga acaactcagg tttatctatc attaatttca aaatatataa
     1141 aagatgatgt acttgtaaaa aacgttaata gtaatcttat acaagat
//
LOCUS       DACEJR010000189         1159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEJR010000189 DACEJR010000000
VERSION     DACEJR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.199"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW651_002989"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW651_002989"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0815632.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          294 a          260 c          181 g          424 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgct
//
LOCUS       DACEJR010000190         1098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEJR010000190 DACEJR010000000
VERSION     DACEJR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.201"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..909
                     /locus_tag="IW651_002990"
     CDS             <1..909
                     /locus_tag="IW651_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ0815633.1"
                     /translation="RRKKLAQMIQENIKEELGLIATVGVGDNPLLAKLALDNEAKHNE
                     GFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRL
                     GVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQV
                     AIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEG
                     QEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEENLDKIIDKIRQKYGFTSIVHASSM
                     LESARSITRSTLVGGHAGGNGGIKND"
     gene            902..>1098
                     /locus_tag="IW651_002991"
     CDS             902..>1098
                     /locus_tag="IW651_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815634.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKE"
BASE COUNT          399 a          169 c          248 g          282 t
ORIGIN      
        1 cggagaaaga agctggctca aatgatccag gagaatatta aagaagagct aggattgatt
       61 gctacagtag gtgtcggaga taatcccctt ttggctaaac tagccttaga caacgaagca
      121 aagcataatg aaggatttgt tgcggaatgg acgtacgaaa acgttcctga aaaagtatgg
      181 aatattcctg aaatgacgga cttttgggga attggctctc gaatgaaaaa aagactcaat
      241 cagatgggga ttatgagcat tagagatctt gctaactgga atccttatac gattaagaat
      301 agactaggtg tgattggctt acagctgtac ttccatgcta acggaatcga tcgaacagat
      361 atagcaatcc cgccagaacc gacaaaggaa aaatcttatg gtaattcaca agtgttacca
      421 cgagattata ctagaagaaa tgaaatcgag ctggtggtaa aagaaatggc ggaacaagta
      481 gccattcgta tcaggcagca taattgtaaa acaggatgtg ttcatttaaa cattggtacg
      541 tcgatcttag aaacgagacc aggattttca caccagatga aaatacctat cacagataat
      601 acaaaggaat tgcagaatta ttgtcttttc ttgtttgata agtattatga aggacaagaa
      661 gtaaggcacg ttggaataac ctatagcaag cttgtgtaca ccgattcgct tcagctggat
      721 ttatttagtg atccacaaaa gcaaattaac gaagaaaatt tagataaaat tattgacaag
      781 attcggcaaa aatatggttt tacgtcgatt gtacatgctt cttcaatgtt agaaagcgct
      841 agaagcatta ctagaagcac acttgttggt ggtcatgcgg gcggaaatgg gggaattaaa
      901 aatgattgat tacaatgatc gtggacgttt aaagtgggca gggttttatt tatctgatca
      961 tactgaagaa attgatgccg acaatgaaca acgaaccagt caaaatttag caaaagaaca
     1021 aatgacgact gaagagattg cggaagtatt gaacgctgca gtcataaaaa atagatcagt
     1081 atctattcaa aaagaaga
//
LOCUS       DACEJR010000191         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEJR010000191 DACEJR010000000
VERSION     DACEJR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.202"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..>1082
                     /locus_tag="IW651_002992"
     CDS             700..>1082
                     /locus_tag="IW651_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0815635.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACEJR010000192         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEJR010000192 DACEJR010000000
VERSION     DACEJR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.203"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>1073
                     /locus_tag="IW651_002993"
     rRNA            2..>1073
                     /locus_tag="IW651_002993"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACEJR010000193         1008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEJR010000193 DACEJR010000000
VERSION     DACEJR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.204"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(309..596)
                     /locus_tag="IW651_002994"
     CDS             complement(309..596)
                     /locus_tag="IW651_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0815636.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(586..915)
                     /locus_tag="IW651_002995"
     CDS             complement(586..915)
                     /locus_tag="IW651_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0815637.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          334 a          180 c          116 g          378 t
ORIGIN      
        1 attacatatt tcttcgctac cacgttccta cctcttttct tttagcaata ttcatttgat
       61 actattataa catatttatt tttagttact accaaaaaaa agatatatat gtactacctt
      121 ataattaata agtaaaaggt agtacataaa taaggaaaag ttgaaatata ttgatttata
      181 gctttcctca aagtaatgac gttatttaat ttatcgagaa gcagagaata ctcgcgactt
      241 tagtcatgta gacgaatcag attttcgata aaaaagaagc cgtccttctt aagaacgaca
      301 acaaatcact agattaattt tttcaactgt tcataaaagt tttcatgcgt tccacataac
      361 aatactggaa taaccttgtt ttctataatc tcgtaagcta ctctatatgt tgtgcccgca
      421 tatttgaacc cactagacca aatacctgat aaatccccag ttttttgttc acccttatat
      481 ggatcaatcg caatttcatc ataaattaaa ttcaaaaatt tttctttcaa tggcttctct
      541 tttagctttt ttaaatactt aatgacatgt tttgaaggaa cgatttcata agccaatttc
      601 tgttgccaac tcctctttag tcattacttt agaattagta agtgtatctt cagcaatatc
      661 tctaaaggaa gaatgcattt tgttttttct attcttaaat tctacaagaa tctcatcttt
      721 atcgtagcct tctgcaatta tatcagacaa aatatcttcg ctaaaatcat aaaattcttt
      781 ttgtggttca acaaattcat aaagaatacc attctctacc aactttgctt caacttcgac
      841 tccattagga acattaaaat ctttaggtac tgtcaacatg attgaattgc cttgttttct
      901 tgtttttgta atcatttaaa tccctcattt ctaacagtat gtaattactt agtaaataca
      961 ttataccacg agataatgta tttacaagta attactgtga ctcgtaat
//
LOCUS       DACEJR010000194          945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEJR010000194 DACEJR010000000
VERSION     DACEJR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.206"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            73..>945
                     /locus_tag="IW651_002996"
     CDS             73..>945
                     /locus_tag="IW651_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0815638.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          331 a          164 c          175 g          275 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataat
//
LOCUS       DACEJR010000195          899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEJR010000195 DACEJR010000000
VERSION     DACEJR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.207"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            167..868
                     /locus_tag="IW651_002997"
     CDS             167..868
                     /locus_tag="IW651_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0815639.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          325 a          158 c          147 g          269 t
ORIGIN      
        1 tcgcttcaaa acaaattatt caataccatt gtatattttt tacataataa caaattatat
       61 ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca aattctttaa
      121 aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc gtgttaataa
      181 aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt tctcggatga
      241 agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag gacagatcct
      301 tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac tcgttcgttt
      361 agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata caaataacct
      421 ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc aagcgatgac
      481 tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc
      541 cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac atgaattgcg
      601 tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt tattcatttt
      661 gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt atccgatcac
      721 cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac aagtaatcaa
      781 acaactacgt gattccgggc tgttagatta tgacaaaaag cgttttactt tctgtaagac
      841 agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga ccctgataa
//
LOCUS       DACEJR010000196          884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEJR010000196 DACEJR010000000
VERSION     DACEJR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.208"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..266
                     /locus_tag="IW651_002998"
     CDS             <1..266
                     /locus_tag="IW651_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815640.1"
                     /translation="SLFWLDTRVPIENDRSKWANLQLQEQEQLNRLLILLTNIATYQS
                     NELGEIIRDSARSQQEIAIINNFQFTEMVHTKAYNRILSIRQF"
     gene            348..>884
                     /locus_tag="IW651_002999"
     CDS             348..>884
                     /locus_tag="IW651_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0815641.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSVVIT"
BASE COUNT          323 a          167 c          156 g          238 t
ORIGIN      
        1 cctcactttt ttggttggat acacgtgtcc ccatcgaaaa tgatcgatca aaatgggcaa
       61 atttacaatt acaagaacaa gagcagctca atcgcttgtt gattcttttg actaatatcg
      121 ctacctatca atccaatgaa ttaggagaaa ttattcgaga tagtgcacgt tcccaacaag
      181 agattgcaat cataaataat tttcaattta ctgaaatggt tcatacaaaa gcctataacc
      241 gcattttaag catacgccaa ttctaaaatt aagctagaca actaaaaaag tcatttgtgc
      301 tacactcaaa atagttccgt cgaaagaatt ataaggagtg agtacaaatg acctataccc
      361 atcttacacc aactgaactc gtgatgatag aagcttattt caatcaatca cactttgttt
      421 caaaagtagc ccatctagta caacgttctc gacaaaccat ttataatgtc tatcgttttc
      481 ttaaatctgg tggaagcgca ataagctact atgaacaata taagaaaaac aaacgtaact
      541 gcggaagacg tccgattgtg ttaccagatg ggcaacaaga atacattcaa aaaaaggtgg
      601 ctcaagggtg gactccagat gtccttattg gtcgagcaga gtttcctatt gcttgttcgg
      661 tacgtacact atatcggatg tttaaagata aaacctttga ccctcacgat ttaccaatga
      721 aagggaaaag aaaaccaaac ggacataaag aaaaaagagg aaaacaggca ttcaaaagaa
      781 atatttctga acgtaaaaaa gattatgtcg ttttcgaaga agaatttgga catttagagg
      841 gtgatacgat tgtcggcatc caccataaaa gtgtcgtcat caca
//
LOCUS       DACEJR010000197          867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEJR010000197 DACEJR010000000
VERSION     DACEJR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.210"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..>867
                     /locus_tag="IW651_003000"
     CDS             170..>867
                     /locus_tag="IW651_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815642.1"
                     /translation="MYDILCNNGFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGL
                     VQWDGSSYPLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVV
                     QPTTVDGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEA
                     GLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIA
                     YDWSAVGWKVIQNP"
BASE COUNT          281 a          128 c          216 g          242 t
ORIGIN      
        1 aggaaatggt aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa
       61 aggaaaagaa ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg
      121 tgggtatcct agtgaattaa aaacagatgc agaaaagaga gcttggagaa tttacgatat
      181 tttgtgtaat aacggattta cgaaacaatc tgcatgtgga atattgggga acattcaaca
      241 agaaacagga ggaacttttg atcctgatac tgttcaaata ggtggaccag catacggatt
      301 agttcagtgg gatggttctt catatcctct tgttggtcca gcaacatggg atggaaaagt
      361 ttatgttcaa aacttattta atgctgcagg tattaaagaa ccaataacga gcttagatgc
      421 acaagttcga ttgcttattt ggacatttac aaatggacaa tggatgggcg tagtacaacc
      481 tacgacggtt gatggattta aggcctgtac tgatccaaga caagcagcat atgcttttga
      541 acgaaactat gaacgtccgg cagcgacaca tcctgaacgt caagattatg cagttaactg
      601 gtataacaaa tttaaagatt taaaaccagg aggagctact ggagaagcag gactaaaaca
      661 tttagagtct ttgattggac aacgaattgg caatggtcag tgttatggct tgtctgcaga
      721 atattcagga tatctgggtg gttgtggcat gggtgctgga acaaaatatg gtttaactca
      781 tgtgatagga aatacttctg cagcatctga tattggtatt gcctatgatt ggtctgctgt
      841 tggttggaaa gtgattcaaa accctag
//
LOCUS       DACEJR010000198          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEJR010000198 DACEJR010000000
VERSION     DACEJR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.211"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(113..721)
                     /locus_tag="IW651_003001"
     CDS             complement(113..721)
                     /locus_tag="IW651_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0815643.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          226 a          149 c          160 g          311 t
ORIGIN      
        1 ttttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac
       61 cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc
      121 agccgtttgc taaccagaat gttttagcag catcccatga accataacga gatgttacat
      181 attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat
      241 ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt
      301 tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag
      361 caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt
      421 cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg
      481 aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt
      541 tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata
      601 atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa
      661 acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca
      721 tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta
      781 gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga attatttaat
      841 atttgt
//
LOCUS       DACEJR010000199          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEJR010000199 DACEJR010000000
VERSION     DACEJR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.213"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW651_003002"
     CDS             24..716
                     /locus_tag="IW651_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0815644.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DACEJR010000200          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEJR010000200 DACEJR010000000
VERSION     DACEJR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.215"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(149..502)
                     /gene="tnpB"
                     /locus_tag="IW651_003003"
     CDS             complement(149..502)
                     /gene="tnpB"
                     /locus_tag="IW651_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0815645.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(492..686)
                     /locus_tag="IW651_003004"
     CDS             complement(492..686)
                     /locus_tag="IW651_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815646.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          221 a          158 c          125 g          238 t
ORIGIN      
        1 ttatgggctt cttgaagcaa ctgaagcaac gtatctgtag tttccaaaat ttttcaccac
       61 cttgaatcga actgattttg ttacagaata ccacaagaaa cgcacaagaa ataggggttc
      121 aacacatttt ttgtgttaac cccccagctt aaaaaactcc tttttttcct ggcaaaatag
      181 ctcgaggttg atcgatactt aatccttcta acaaccatcg aacctgttgg ttcgttaagg
      241 ttcgaacttc gtctttcgtc cgaggccatt ttaacttgcc gttttctatc cgcttataac
      301 agagaagaaa accatcaccg tcccaataga gaagtttgaa acggtcttga cggttcccgc
      361 aaaataaaaa aacggcatct tcgtaaagct cgagttgata ctcttgttga ataagactgg
      421 caagaccatc aattcctttt cgcaaatctg tttttccgca cacaatataa atatgtttgg
      481 tttgaagtaa atcataaagc attggacaac agttctttca aaatagcttg aagaatataa
      541 ttgttaatac cgttatagat caaaacttca gaaccattct ctcttttgat gcgcatcgct
      601 aacttggcag ccgcgggttt acctttttct ggtgcactct tttcgtttcc taattggaca
      661 ggaacaatct catttaattt atacatagaa aacctccaga cttcttgata gctcaagtat
      721 atcttgaggt gcatttgtta gt
//
LOCUS       DACEJR010000201          605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEJR010000201 DACEJR010000000
VERSION     DACEJR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.218"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>605
                     /locus_tag="IW651_003005"
     CDS             <1..>605
                     /locus_tag="IW651_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0815647.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFN"
BASE COUNT          173 a          129 c          137 g          166 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataa
//
LOCUS       DACEJR010000202          585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEJR010000202 DACEJR010000000
VERSION     DACEJR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.219"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          184 a           93 c           90 g          218 t
ORIGIN      
        1 taaatattat acagataata acggaatatt atcatcgaaa cagctaacaa aattgtcata
       61 aaaaacgaga tggttgatat tagaacttgt gtttttgttt aggtctagag ataaagagag
      121 acaatttcca aatatatgta atgaatacaa aaagacgttt gtcgcggatt accgttacaa
      181 acgtctttta ttgattttcc gagatctcat cagtcgccat ttggaatggt tcactcctta
      241 tctagattcg tttaatgctc ggactgcttg ttcgattgta ataccaaacg caacgttatt
      301 ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt gtatcaaaag ctacgcggta
      361 ttgcatattt gtttttgtaa acgtcataaa attcatctcc tttgtaatgt aatttagttc
      421 ttgaaagttt cctttaaact ttcgaactta taccaagttt agcatcgtac gttttttgat
      481 gcaattaaaa agcatttatc attttcctta tttatttttt cttaaatggt ttagctattt
      541 tcttttaatc cgataagtag cccaatcatc aaggttacaa atact
//
LOCUS       DACEJR010000203          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEJR010000203 DACEJR010000000
VERSION     DACEJR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.220"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>575
                     /locus_tag="IW651_003006"
     CDS             <1..>575
                     /locus_tag="IW651_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0815648.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKG"
BASE COUNT          238 a           50 c          103 g          184 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggt
//
LOCUS       DACEJR010000204          569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEJR010000204 DACEJR010000000
VERSION     DACEJR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.221"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..569
                     /locus_tag="IW651_003007"
                     /pseudo
     CDS             1..569
                     /locus_tag="IW651_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          207 a          114 c          121 g          127 t
ORIGIN      
        1 attattcgag agtatgagct tacgccttct tctttcgata aatggatgaa gcaagcacaa
       61 tcaacgggct cattcaaaga aagagacaac ttaacaccag aacaagcaga attgatcgca
      121 ctaagaaaga aaaataagca actcgaaatg gagaatgata ttttaaagca agcggcgctg
      181 atattcggac gaaaagacaa gtaattgatg ccaacaagca taaatattcc atatcagcga
      241 tgtgcaaaat tctaaatatt tctcgtcaga cctactatta tcaagcgaaa ccgatcgaaa
      301 atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga aaggcttacg
      361 gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa
      421 tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct cattttaaag
      481 ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg acctttacac
      541 aagaacagcc attggaagcc atcgttacg
//
LOCUS       DACEJR010000205          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEJR010000205 DACEJR010000000
VERSION     DACEJR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.223"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..199)
                     /locus_tag="IW651_003008"
     tRNA            complement(128..199)
                     /locus_tag="IW651_003008"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Glu,seq:ttc)
     gene            complement(211..284)
                     /locus_tag="IW651_003009"
     tRNA            complement(211..284)
                     /locus_tag="IW651_003009"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Ile,seq:gat)
     gene            complement(297..367)
                     /locus_tag="IW651_003010"
     tRNA            complement(297..367)
                     /locus_tag="IW651_003010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Gly,seq:tcc)
     gene            complement(372..444)
                     /locus_tag="IW651_003011"
     tRNA            complement(372..444)
                     /locus_tag="IW651_003011"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Phe,seq:gaa)
     gene            complement(448..520)
                     /locus_tag="IW651_003012"
     tRNA            complement(448..520)
                     /locus_tag="IW651_003012"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(485..487),aa:Asp,seq:gtc)
BASE COUNT          169 a          137 c          107 g          108 t
ORIGIN      
        1 taaaaatatt ttacattaga actgtccaaa aaagcttagc acaatcatcc ataaagataa
       61 ctctgctaag ctttttaaaa tcattctttc agataaaaag aaaaagatac tcaaaaagta
      121 tcttccatga cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta
      181 accgcttgac caacgggcca ataaaataaa tgggcctaaa tggactcgaa ccatcgacct
      241 cacgcttatc aggcgtgcgc tctaaccagc tgagctatag gcccataaaa actaaaagcg
      301 ggtgacgaga atcgaactcg cgacaacagc ttggaaggct gtggttttac cactaaacta
      361 cacccgcata atggcgcggg acagaatcga actgccgaca catggagctt caatccattg
      421 ctctaccaac tgagctaccg agccaaacgg ttccgacggg aatcgaaccc gcgatctcct
      481 gcgtgacagg caggcatgtt aacccctaca ccacggaacc a
//
LOCUS       DACEJR010000206          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEJR010000206 DACEJR010000000
VERSION     DACEJR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.denovo.225"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            64..>498
                     /locus_tag="IW651_003013"
     CDS             64..>498
                     /locus_tag="IW651_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0815649.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEV"
BASE COUNT          160 a          115 c          105 g          118 t
ORIGIN      
        1 aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc
       61 catatgaacg attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat
      121 gaattattcc gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg
      181 gctttcctcg attacgaaaa gtacgatcgc attggtttta acacgggtaa ttctcgtaac
      241 ggctcctatg accgtacggt caagaccgag tacggggaac ttcatctcca gattccgcgc
      301 gaccgcaacg gcgagttcaa gcaacagact gttcctgctt atagacggac gaatgatacc
      361 ttagaggaga ccgtcattca cctcttccga aaaggtatta ccatgtcgga aatcgcagac
      421 ttgattgaga aaatgtatgg gcatcactac acgccccaaa ccatgtccaa catgactaaa
      481 gtgctgactg aagaagta
//
LOCUS       DACEJR010000207        13648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.072, whole genome shotgun sequence.
ACCESSION   DACEJR010000207 DACEJR010000000
VERSION     DACEJR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.072"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..564
                     /locus_tag="IW651_003014"
     CDS             289..564
                     /locus_tag="IW651_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815650.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IW651_003015"
     CDS             577..2655
                     /locus_tag="IW651_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0815651.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IW651_003016"
     CDS             2815..4701
                     /locus_tag="IW651_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0815652.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IW651_003017"
     CDS             4713..5660
                     /locus_tag="IW651_003017"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0815653.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IW651_003018"
     CDS             5680..6207
                     /locus_tag="IW651_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0815654.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IW651_003019"
     CDS             complement(6270..6923)
                     /locus_tag="IW651_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0815655.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7055..7897
                     /locus_tag="IW651_003020"
     CDS             7055..7897
                     /locus_tag="IW651_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0815656.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8055..8951
                     /locus_tag="IW651_003021"
     CDS             8055..8951
                     /locus_tag="IW651_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0815657.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8954..9667
                     /locus_tag="IW651_003022"
     CDS             8954..9667
                     /locus_tag="IW651_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0815658.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9687..10205
                     /gene="msrA"
                     /locus_tag="IW651_003023"
     CDS             9687..10205
                     /gene="msrA"
                     /locus_tag="IW651_003023"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0815659.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10202..10429
                     /locus_tag="IW651_003024"
     CDS             10202..10429
                     /locus_tag="IW651_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0815660.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10581..11132
                     /gene="lepB"
                     /locus_tag="IW651_003025"
     CDS             10581..11132
                     /gene="lepB"
                     /locus_tag="IW651_003025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0815661.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11215..11757)
                     /locus_tag="IW651_003026"
     CDS             complement(11215..11757)
                     /locus_tag="IW651_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0815662.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11994..12392)
                     /locus_tag="IW651_003027"
     CDS             complement(11994..12392)
                     /locus_tag="IW651_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0815663.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12652..13512
                     /gene="ylqF"
                     /locus_tag="IW651_003028"
     CDS             12652..13512
                     /gene="ylqF"
                     /locus_tag="IW651_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0815664.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13505..>13648
                     /locus_tag="IW651_003029"
     CDS             13505..>13648
                     /locus_tag="IW651_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0815665.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKE"
BASE COUNT         4765 a         2326 c         2763 g         3794 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
     6961 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
     7021 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
     7081 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
     7141 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
     7201 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
     7261 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
     7321 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
     7381 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
     7441 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
     7501 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
     7561 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
     7621 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
     7681 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
     7741 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
     7801 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
     7861 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
     7921 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
     7981 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
     8041 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
     8101 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
     8161 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
     8221 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
     8281 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
     8341 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
     8401 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
     8461 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
     8521 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
     8581 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
     8641 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
     8701 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
     8761 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
     8821 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
     8881 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
     8941 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
     9001 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
     9061 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
     9121 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
     9181 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
     9241 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
     9301 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
     9361 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
     9421 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
     9481 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
     9541 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
     9601 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
     9661 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
     9721 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
     9781 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
     9841 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
     9901 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
     9961 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    10021 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    10081 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    10141 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    10201 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    10261 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    10321 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    10381 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    10441 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    10501 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    10561 gagggatgga ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg
    10621 tattcaaata cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca
    10681 tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg
    10741 tgatggaaaa attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg
    10801 gcacgattta tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg
    10861 atcaattgaa gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat
    10921 cagatcatgc aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat
    10981 ccaagcttcc ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata
    11041 gtcgttcgtt tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt
    11101 atccacttga tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag
    11161 actgcacgct ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac
    11221 agctcgttta aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat
    11281 ttgaataggg atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc
    11341 tgcaccaaac aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat
    11401 caataattgg gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc
    11461 attaataaat ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc
    11521 aaatatcgtg aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc
    11581 atatagataa cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg
    11641 accaatcgct atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa
    11701 caatcttgca ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat
    11761 attctctcct ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac
    11821 acattggtga tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt
    11881 ttataaactg taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc
    11941 gtatcatccg cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc
    12001 ttcatccata cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt
    12061 tcttcttcag gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt
    12121 tgagcaaatt tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt
    12181 gtttttttat tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag
    12241 agaagcatta tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat
    12301 tcttcatttt ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca
    12361 acaggaaaat taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg
    12421 tctatcatct cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa
    12481 ataaaaattt gcatcatcaa aaaagtgaag atgtgataga atgaggactg gttcactttc
    12541 acttaactaa tagatggaat ttctttataa taaaaaagtg aatttatatt gaaaaagaaa
    12601 aaaacaagta aagccgggga atattccggt atgaaaggaa tggagagaac aatgaccatc
    12661 caatggtttc cgggacatat ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat
    12721 gtggacatcg tatttgaatt ggtagacgca cgattacctt tatcttctcg taatccaatg
    12781 ctagatcaga tcattcagca aaaacctcgc cttgttttat tgaataaagc ggatctggca
    12841 gatccacaac agacaaagct gtggcaagaa tattttcgta aacaaggcca tcttgcatta
    12901 gctatcacgg cacaagaaag taaaggaatt aaaggtctag tacctgcggc taaagaagca
    12961 ttgaaagaaa aacgggcaag agatgctgct cgggggatca agcctcgcgc tattcgggca
    13021 atggtcttag gtattccaaa tgttgggaaa tctacgttga tgaatcgact agtaggcaag
    13081 aaaattgcac agacaggaaa taagcctggc gtaacaaaag gtcagcagtg gcttcgttta
    13141 ggtaatgagt tggaattgtt agacacacca gggattttat ggcccaaatt tgaagatgaa
    13201 gaaatcggca aaaaactagc gctgacaggc gcgatcaaag accagcttgt ccatttagat
    13261 gatttagcga tttacggatt agactttttt gcccgatatt attcaggaag aatcagcgat
    13321 cgttatggtt tatcaatcga gcaagaagaa aaattggcac cggaattact gatggatatt
    13381 acagcaaaac gtgggtataa agaagactat gatcgtggca gcgaattagt cgtgcatgat
    13441 attcgtcaag gaaaacttgg acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa
    13501 agtgatgact gaatccatcc aaagtatcaa agaacgattg aaaagcatac agagaataga
    13561 tgatcctaca ctagaatcat taagaaatga tccacgcaaa ggcgtgcagc aagcactggc
    13621 ttcttttgaa cgacgattga aaaaggaa
//
LOCUS       DACEJR010000208        11422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.089, whole genome shotgun sequence.
ACCESSION   DACEJR010000208 DACEJR010000000
VERSION     DACEJR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.089"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            39..977
                     /locus_tag="IW651_003030"
                     /pseudo
     CDS             39..977
                     /locus_tag="IW651_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IW651_003031"
     CDS             980..3163
                     /locus_tag="IW651_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0815666.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IW651_003032"
     CDS             3160..4161
                     /locus_tag="IW651_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0815667.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IW651_003033"
     CDS             4176..5090
                     /locus_tag="IW651_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0815668.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IW651_003034"
     CDS             5335..5451
                     /locus_tag="IW651_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0815669.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW651_003035"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW651_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0815670.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW651_003036"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW651_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0815671.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IW651_003037"
     CDS             9421..10683
                     /locus_tag="IW651_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0815672.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IW651_003038"
     CDS             10928..11410
                     /locus_tag="IW651_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0815673.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1907 c         2388 g         3356 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACEJR010000209         9667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.096, whole genome shotgun sequence.
ACCESSION   DACEJR010000209 DACEJR010000000
VERSION     DACEJR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.096"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..466)
                     /locus_tag="IW651_003039"
     CDS             complement(23..466)
                     /locus_tag="IW651_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0815674.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(684..2024)
                     /locus_tag="IW651_003040"
     CDS             complement(684..2024)
                     /locus_tag="IW651_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0815675.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2293..3189
                     /locus_tag="IW651_003041"
     CDS             2293..3189
                     /locus_tag="IW651_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0815676.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3339..4280)
                     /locus_tag="IW651_003042"
     CDS             complement(3339..4280)
                     /locus_tag="IW651_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0815677.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4406..5527
                     /locus_tag="IW651_003043"
     CDS             4406..5527
                     /locus_tag="IW651_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0815678.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5785..7287
                     /locus_tag="IW651_003044"
     CDS             5785..7287
                     /locus_tag="IW651_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0815679.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7354..7881
                     /locus_tag="IW651_003045"
     CDS             7354..7881
                     /locus_tag="IW651_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0815680.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8092..9153
                     /locus_tag="IW651_003046"
     CDS             8092..9153
                     /locus_tag="IW651_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0815681.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9156..>9667
                     /locus_tag="IW651_003047"
     CDS             9156..>9667
                     /locus_tag="IW651_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0815682.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCN"
BASE COUNT         3252 a         1757 c         1963 g         2695 t
ORIGIN      
        1 tttttatttg gacggataat ggtcattcca tttctttgat catttttttc attttgtcga
       61 tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca ggatctttta
      121 aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg gccttcatcc
      181 aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg gcaaaaacaa
      241 gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt ttccacatga
      301 acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat actcgtgtac
      361 gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca aacataacta
      421 ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa aacaaactcc
      481 tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa ttattttgca
      541 cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc ggaaaaagga
      601 gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat tccacataaa
      661 taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc attctttttt
      721 tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac cccaatggag
      781 ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa aaagctgatt
      841 cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc actgataatg
      901 atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa taatttgtca
      961 ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa taaaacagca
     1021 cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc ttttcgatcc
     1081 ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat agatgagaac
     1141 aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt cattttttca
     1201 aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg aaaaagaatc
     1261 aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac acctttgatt
     1321 gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc aatcagttca
     1381 gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt cataccatga
     1441 ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc tgtgcccaac
     1501 atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac actgatttta
     1561 gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag attgatgatc
     1621 gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg gaaccacaac
     1681 tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca catagaccag
     1741 tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat caaaccagat
     1801 aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt catcacgata
     1861 tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc gatcccaatc
     1921 gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc taacatggtg
     1981 atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga cctctttctt
     2041 ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca atgactaatg
     2101 ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat tttaatagaa
     2161 gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa atataggaaa
     2221 cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga cagagaaagt
     2281 gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa atcactttat
     2341 tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa
     2401 ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt tttagattat
     2461 ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta
     2521 tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa
     2581 ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact tgggctgaaa
     2641 cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc aaagacggat
     2701 ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta taagcaggaa
     2761 tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg gaatgaacaa
     2821 cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg cgggattctg
     2881 gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc catgtatttt
     2941 caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta tcgtcctgaa
     3001 aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct gcatacacca
     3061 tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg ggcaaaacga
     3121 tttatcacta acgattccac tatttatgag caatacgaat atgacctctc ctctgttgaa
     3181 aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa gcacaattct
     3241 tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa aaaaagagaa
     3301 ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc aaggattcct
     3361 tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt
     3421 tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg atggtttttg
     3481 atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa
     3541 gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc tgcgatagaa
     3601 gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc gcctagttca
     3661 gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac acaagctttt
     3721 cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc ttcttgaaag
     3781 acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt
     3841 tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg
     3901 atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc atgacctaat
     3961 ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat cgaacaaaac
     4021 atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg ttcgttgctt
     4081 tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt accgttaaag
     4141 tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg atcgatcaag
     4201 aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat tgctccggcc
     4261 ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat
     4321 atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta
     4381 cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg
     4441 aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg
     4501 aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta
     4561 cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa
     4621 aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca
     4681 agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag
     4741 ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt
     4801 cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc
     4861 ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga
     4921 tcgaaaaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc
     4981 atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac
     5041 gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg
     5101 ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct
     5161 ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt
     5221 ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca
     5281 aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc
     5341 aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc
     5401 aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca
     5461 aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc
     5521 gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg
     5581 agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt
     5641 gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg
     5701 gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5761 tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc
     5821 tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg
     5881 aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa
     5941 aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa
     6001 cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag
     6061 atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga
     6121 gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg
     6181 gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga
     6241 aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac
     6301 cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa
     6361 ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa
     6421 atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa
     6481 aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc
     6541 gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg
     6601 aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta
     6661 ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag
     6721 cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt
     6781 cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag
     6841 tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac
     6901 cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg
     6961 ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga
     7021 aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa
     7081 cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat
     7141 gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac
     7201 gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact
     7261 gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt
     7321 aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa
     7381 acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa
     7441 ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg
     7501 aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt
     7561 ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat
     7621 ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc
     7681 ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc
     7741 atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc
     7801 aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca
     7861 gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca
     7921 ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc
     7981 aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga
     8041 tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc
     8101 ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg
     8161 gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg
     8221 gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg
     8281 atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg
     8341 cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag
     8401 attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa
     8461 gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat
     8521 tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg
     8581 ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc
     8641 ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc
     8701 gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca
     8761 gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg
     8821 gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac
     8881 gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca
     8941 gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg
     9001 cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat
     9061 gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg
     9121 aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc
     9181 taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag
     9241 ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga
     9301 caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga
     9361 tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac
     9421 cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa
     9481 tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca
     9541 agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat
     9601 atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt
     9661 gcaataa
//
LOCUS       DACEJR010000210         8094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.105, whole genome shotgun sequence.
ACCESSION   DACEJR010000210 DACEJR010000000
VERSION     DACEJR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.105"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..182)
                     /locus_tag="IW651_003048"
     CDS             complement(<1..182)
                     /locus_tag="IW651_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAQ0815683.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLL"
     gene            complement(524..1396)
                     /locus_tag="IW651_003049"
     CDS             complement(524..1396)
                     /locus_tag="IW651_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0815684.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1524..2093
                     /locus_tag="IW651_003050"
     CDS             1524..2093
                     /locus_tag="IW651_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0815685.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            2160..2897
                     /locus_tag="IW651_003051"
     CDS             2160..2897
                     /locus_tag="IW651_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0815686.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            3128..4396
                     /locus_tag="IW651_003052"
     CDS             3128..4396
                     /locus_tag="IW651_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0815687.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4883..5347
                     /locus_tag="IW651_003053"
     CDS             4883..5347
                     /locus_tag="IW651_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0815688.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            5331..5960
                     /locus_tag="IW651_003054"
     CDS             5331..5960
                     /locus_tag="IW651_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0815689.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            6314..7792
                     /gene="msr"
                     /locus_tag="IW651_003055"
     CDS             6314..7792
                     /gene="msr"
                     /locus_tag="IW651_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0815690.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7839..>8094
                     /locus_tag="IW651_003056"
     CDS             7839..>8094
                     /locus_tag="IW651_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0815691.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISC"
BASE COUNT         2700 a         1439 c         1626 g         2329 t
ORIGIN      
        1 agtaaatttt gcaacttgat ttgaccttct ggttccatga caaatatccg ttcgacatca
       61 cgatcaaaaa tcaaatcatt tctttttctg tcaaatccaa tcccttttcc aatagctact
      121 ttttcctgtc caccgtcatc taccaatact gcattttgat taaagatctt tttgattttc
      181 atgcgcgcac cctttttgaa gatttggttt tcacgtttgc tagaccagtt gattgcggcc
      241 aaacaaaaat agacacgaaa aacctcctag ggaaaccatg ttcctcagag atttttcatg
      301 cctagtatga tctagtcaca tgaaacgaat tattgttaac gtaatcaatt ttatatagac
      361 agttatgttt tgtcaattct ttttttataa ttatagtaat ttactggaat tatcagaaat
      421 aaaaggaaga aataaattaa gaaacttatg ttatttaatc gttggatctt ttgcttatct
      481 tattcgttgt taaaaagctg aaagcgaagg aaaaaaactc tacttaactg tatttctccg
      541 ttaggacatc taaaaattta cgagcagcag gagaaagagg ctggtctttt ttccaaatga
      601 gattcagtga agcttgtaat ggcggatcga gtgggatgaa tattttttcc gtcccataag
      661 tattgatgat cccatctaga gctaatgcac caaaagtccc gttatcgacc atcaaagaag
      721 cattgtacaa taagttatat gttccgacga tcgataactg atctatgctg aaacctagcc
      781 attcagctag ttgactgtcc actgacgttt gccgggaaat gatcaaagga acagttttca
      841 gttcctgcgg atggatgacg gtttgctttg ctaaaggatg atttcgatgt atcaaaagtc
      901 cccaacgatc ttgggctggc agtcgaaatg actggtattt ttgtttgttt gtcggttcga
      961 ttacgagtcc aaaatcaagt aatccgctat ccagtttgtc tatgatatca tctgcattcc
     1021 cgctatataa atgaaactgg atatcaggat gcgccttgat caacgaatga aggctatcag
     1081 aaatgaaaga gattgcttta gtttcgcctc cgccaatgta gatttctcca gagatgattg
     1141 cgtcatttgt taaattgttc gccgtttttt ctaccaacga caagatttct tttccttttg
     1201 aataaagata tcgaccagaa tccgtcaact gtatctctcg ggagccccga gtgaaaagtg
     1261 tcacaccaag ttcttcttct aaatctttca gttgttttga gagagtaggt tgcgtgatat
     1321 gcagagattt tgctgctcgg ctgatatttt tttctctagc tactgctaaa aaatacgtca
     1381 gtatacgaat atccattttt tccctcctgt ttttaaaaaa gcataccact aagtatagtt
     1441 aaaaggaata gttaagtatg ttttataagt attagacatg ataagccgtc cgttataaag
     1501 taaggataaa aggagggagc aaaatgacaa gcctcatcga aaaacaaatc gtcaataaag
     1561 agattcgagt aacggatccg ctctttgaag tgatccatca gatccaagct gaaaacgaag
     1621 agcctttagc agcgctgaat actgggtacc atgaaccagc agatatccgc aaacggctag
     1681 aaaatatcat tagtgacaaa gtagatgata cggtgactgt tctgctgcct ttttatacag
     1741 atttcggcaa acatattagt atagggaaaa atgtctttat caatcgtcaa gtgatgttcg
     1801 ttgacttagg tggcatctgt ttagaagatt cagtattgat tggtcctcgt gtcaacctca
     1861 tcacggtgaa ccatctaacc gatccaaaag aacgaagagg cttgtcagta aagccaatcc
     1921 acatcaagaa aaatgcttgg atcggagcag gagcaactat tctgccaggc gtaacgattg
     1981 gcgagaatgc cattgttgca gcgaatgcaa ctgttacgaa agatgtccca gacaatacca
     2041 tcgttgcagg aatccctgcc aaaatcgtca aacctgtcga acggattatt tgaacagtaa
     2101 gatgaaataa gagatagtga gtataagcaa gagatttttc aaaacaggag gagaatagaa
     2161 tgaatgattt atcagaaaaa gtgattgtca tcatgggcgc ttcaagcgga atcggtgaag
     2221 caacaacgaa gctattagca gaaaaagggg ctaaactagt tatagctgca cgccgagaag
     2281 atcgcctaaa agcaataaaa gaatctttac cagaagctga gctatatatt caaacagcag
     2341 atgtaagaga ttttgcacaa gtccaagcag ttatcgattt agcgatggaa aagttcggtc
     2401 gaatcgatgt attgtacaat aatgcaggaa tcatgccaac cgctccatta gttgaaggtc
     2461 accgtgatga atggcaaaat atgcttgata tcaacatcat gggtgtgtta aatggaattt
     2521 ccgccgtact tccaatcatg gaaaaacaaa aatctgggca tatcatttca actgattctg
     2581 tggcgggaca tgttgtttat cctgattccg ccgtttattg cggaacaaaa tttgctgtcc
     2641 gtgcaatcat ggaaggatta cgtcaagagc aacggcaaaa taacatcaaa tctacgatca
     2701 tctctccagg agctgttcaa actgaattat atcaaaccat cagcgacaaa aaggctgctc
     2761 ttgaacttca tgaagcgcaa aaagaatggg gattgacctc cgaagatata gcttctgctg
     2821 tagcatttgc gatagaaacg ccagaccgga tgtctgtcag taacatgatt atccgtccga
     2881 cgacacagga agtataatcc ataacggata atatccaaag agaattactt gttaaatagt
     2941 tattcaaacg accatcaatt taaggcgtga atggagaaga atattcttca tttacgtctt
     3001 tttatgtaga aaaagcaaat ttatcaaaaa gaaacaaatg ttatttaaat atggacattg
     3061 acttgctttt acttttttag ttacactgtt atttaggaaa agtttcataa ttcaaaggag
     3121 aaaaaagatg aaagcaaaga atttattatt tagcgttatt cttttaggaa atctagcagt
     3181 tgccaacaca acagtgctag ctgaagaatc gacagaaact gtcgcaactc agacatcgag
     3241 ttcttcagaa acaggaattg aaaacgatac agagagtact ttgcagtcag ctactgtctc
     3301 agaaaataca atttcaaccg aagaaccaac aactgaaaca acagaacaaa cacaaaccga
     3361 agacagtaca tcagcagaag ctcagaacaa tccagagaat gcagaagaaa tcacgaacca
     3421 attgacagat gggcaaacat tgacacatac ccatcaagca gatttgtctg aattcgaaaa
     3481 ctatactggc tatgcttttt atgctcgctc atctgctaat tctcaacaag cgttcattga
     3541 tagtattgcc tcaactgcac aaagtttggc aagtgccaat gacttatatg catcagtcat
     3601 gattgctcaa gccatcgttg aaagtggctg ggggaacagt actttggctt ctgcccctaa
     3661 ttacaatcta tttgggatta aaggaagcta taatggccaa tctgttacga tgcctacttc
     3721 ggaatatgtg aatgggcaat ggattacggt gaatgccgct tttagaaaat atccttcgta
     3781 caaagaatca ttgcaagata acgtaacggt attgaagacg acttcttttc agccaggcgt
     3841 ttactactat tccggtgcat ggaaaagcaa tacgaactct tacaaagatg ctacggcttg
     3901 gctaacaggt cgttatgcca ctgcgcctaa ttatggttct acattgaaca atgtaatcga
     3961 aacctataat ctgacacagt atgatacagc accaacttca acttctccga tgtatcgtct
     4021 gtataaccgt catacaggag aacatttata tacattgaat gccggagaaa aaaattatct
     4081 gccaactgta ggctgggaat atgaagggat tgcttggcaa gcaccaaaca gcggacaacc
     4141 tgtctaccgt ttatacaatc cttacagtgg agaccatcat tacacaatgg cacaatctga
     4201 gatcaactct ttaacaaaaa tcggttggcg ttatgaaggt ctaagttttt attctggtgg
     4261 ttccaagcca atttatcgac tatttaatcc aaatgaaaaa actggtaccc atcactatac
     4321 cttgagtgct aaggaaagag attggctaac accgatgggc tggagatatg aaggaatagg
     4381 attttatggt tattaaaaag aggtagtgaa aaagctgccc tgcatcaagt agtaagaaca
     4441 gccaaacaaa aacagatttt catgtttttt gtttggttga acttattacc gcagatgcaa
     4501 gtttttgaac accgtttact caaaaaaggt tgtgacaaca tcttgtcata acttttttat
     4561 tactgtttaa gcatactgat ccaatttcta atattcgacg ggattcttgc aaaaggcgat
     4621 ttcggaaatt tttggcggtg taaaattttc tactgcagaa gagctatctt ctatagtttg
     4681 ataagaatca ataatcttgg ttttagcgtt gtgctttctt tatcattttt tccgttcatt
     4741 ctacaaaaaa tttcttttct ctatactttt ctattgagag cgtttttttt attacacagc
     4801 tgattctttg acttttttct ttttttattc tattattgac aagtcaatcg ttgacgagtc
     4861 aattattaga aaggaggaaa ttatgtcttt agaagagatt agtcaccttt tatatcaaat
     4921 caaattagcc gatcaaaaaa tcgtccagat ctttgaagaa aatatcggta taagtcttac
     4981 tagatacgag atcatgatga gactgaaaga agaaaatcca ttgctacaaa gcagtttgca
     5041 agaaaagttg cagattgatc aaggagcagt tactcgtcat ctgaaggtat tggaagcaaa
     5101 tggctatgtt tcgcgtcaga gaaatcctca gaacaaccgg gaagtgtatg tagcgctaac
     5161 agaaaaagcc cagaagaaaa tcgagcattg tgaaatggac cataaagata caggttccgt
     5221 gctatccccg gctttcagcg atgtagaaat gaaacaattt ctaacacttt tagataaatt
     5281 gaatacccaa ctttgcaata taacaacaga aacgaaagag gttaataata atggaacaaa
     5341 aaaatgattt ttcaacaatt atgactggta gaagatccgt tcgcgtatat gatgaaacat
     5401 acaaaatccc acacgaagaa atgctggaaa tgatcaaaga agcgacgact gcaccttctt
     5461 ctgtcaatat gcaaccatgg cgctttgtgg tcgctgaaag cgaggaagca aaagaaatct
     5521 tgcgtccttt gatccgtttc aatacgagac aaaatgatac ttcttctgca atggtcatga
     5581 tttttggcga tatgcaatgt tacgaatatg gcgaagaaat ctataaccaa gcttacgaat
     5641 cagggaagat gccaaaagaa gtcagagatc aacaattagc tgcgatcatt ccttattaca
     5701 agagtttttc aagagaagaa atgaacgatg tagtcaaagt cgattctagc ttagcggcta
     5761 tgcaattcat gttagttgcg cgtgatcatg gctatgaaac aaatccaatt ggtggattcg
     5821 aagcagatca gttggcagag gcatttggtt tggataaaga ccgttatgtt ccagttatta
     5881 ttttatcaat aggaaaagcg gtagaagaag gttacgagtc tgttcgttta gcaccagaaa
     5941 agatcactac gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg
     6001 aatcgtgtgt ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt
     6061 acgtttcgaa cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt
     6121 tcatggatgg agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag
     6181 atttggtgat ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt
     6241 ttgcaaacag atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag
     6301 gagtgtaaaa catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg
     6361 caaccagcta gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat
     6421 cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt
     6481 tcccgatcat gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta
     6541 tctcgctgaa gcaaaggacc taaatttgga attagccagt catttccagt taagactgga
     6601 agaaacttca gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct
     6661 tctttcttct tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa
     6721 aagtattgat cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag
     6781 tcatgatcgc tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga
     6841 aatccgagag ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa
     6901 gactcagcta cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat
     6961 ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca
     7021 acaaatcaga ccggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat
     7081 ccaaaagaat gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc
     7141 gcagcagatg aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta
     7201 tcccatcatg ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg
     7261 tgattttcag ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa
     7321 gacaacctta ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt
     7381 aagctttcaa gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg
     7441 ttttgtcatg agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt
     7501 agggtttgct caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac
     7561 ccgtctgacg attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc
     7621 gactaatttt attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc
     7681 gggaacgatt ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt
     7741 ttatgaaata aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta
     7801 cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag
     7861 gaataatttt tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt
     7921 tatggctgca tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg
     7981 tggtggcttt cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta
     8041 aactaccagt aaatccagaa agcaaggtcc aaatgatctt aactatttca tgct
//
LOCUS       DACEJR010000211         7482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.109, whole genome shotgun sequence.
ACCESSION   DACEJR010000211 DACEJR010000000
VERSION     DACEJR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.109"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW651_003057"
     CDS             109..192
                     /locus_tag="IW651_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0815692.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW651_003058"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW651_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0815693.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW651_003059"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW651_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW651_003060"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW651_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW651_003061"
     CDS             2348..2593
                     /locus_tag="IW651_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0815694.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW651_003062"
     CDS             2696..3565
                     /locus_tag="IW651_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0815695.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW651_003063"
     CDS             3546..4280
                     /locus_tag="IW651_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0815696.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW651_003064"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW651_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IW651_003065"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IW651_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0815697.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IW651_003066"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IW651_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0815698.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IW651_003067"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IW651_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IW651_003068"
     CDS             6927..7469
                     /locus_tag="IW651_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0815699.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACEJR010000212         7194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.111, whole genome shotgun sequence.
ACCESSION   DACEJR010000212 DACEJR010000000
VERSION     DACEJR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.111"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..799
                     /locus_tag="IW651_003069"
     CDS             <1..799
                     /locus_tag="IW651_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0815700.1"
                     /translation="ELEMPGKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDV
                     YMWGKYPIAAMRFLEEKGWAPTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVG
                     AGKMNTTGKKGSSEETGVPGMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMP
                     VLITENGLGEYDKLTEDKQIHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLL
                     SWLNGYQKRYGFVYVDQDETQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            988..3435
                     /locus_tag="IW651_003070"
     CDS             988..3435
                     /locus_tag="IW651_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0815701.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            3665..4732
                     /gene="rlmN"
                     /locus_tag="IW651_003071"
     CDS             3665..4732
                     /gene="rlmN"
                     /locus_tag="IW651_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0815702.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4925..6028)
                     /locus_tag="IW651_003072"
     CDS             complement(4925..6028)
                     /locus_tag="IW651_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0815703.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            6581..7129
                     /gene="aac(6')"
                     /locus_tag="IW651_003073"
     CDS             6581..7129
                     /gene="aac(6')"
                     /locus_tag="IW651_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0815704.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2421 a         1233 c         1561 g         1979 t
ORIGIN      
        1 cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa attatccaat
       61 tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga tggccaatta
      121 ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt tcttagaaga
      181 aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc
      241 agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc cattaaacgg
      301 agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag aaactggagt
      361 tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt gggactggga
      421 gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt accgcatgcc
      481 ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag ataaacaaat
      541 ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca aagaagcaat
      601 ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt tgcttagctg
      661 gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg aaacacaaga
      721 aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac tgattgaaga
      781 aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg gatatggccg
      841 tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa gctgaaaaat
      901 gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt tttgtataat
      961 gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac taaaaaaagg
     1021 aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct gacgattggt
     1081 gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg ttttttagga
     1141 atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat cctctgtctt
     1201 ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc aattggtaaa
     1261 aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt gttacatgcc
     1321 catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac atgggatttg
     1381 ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg gatgattgga
     1441 gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta tttagcagca
     1501 gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta tcagctgtta
     1561 aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga tcctgccaag
     1621 caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa
     1681 gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata tgagaagaaa
     1741 aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc agaaaataca
     1801 gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat ccatcctgcg
     1861 aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga aggtttttca
     1921 gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc agaacaggaa
     1981 aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac tgtggatcaa
     2041 agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac tttccaaagt
     2101 ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac aaaattcgaa
     2161 gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga tgatatcgcg
     2221 cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa atcactgatt
     2281 ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat catcgaagca
     2341 caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt ttctggacgg
     2401 gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc gactggtagt
     2461 gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc taagccccat
     2521 gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta caacggcata
     2581 cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc cttacaaaaa
     2641 gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt aagaaatatt
     2701 tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa acatccaacc
     2761 ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt agccagcaat
     2821 gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg gattcatatg
     2881 atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa agcaaatgta
     2941 ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat cattgattct
     3001 aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat gggtgagaat
     3061 aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg ggtcgttcaa
     3121 tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac agatgaagtt
     3181 gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc aaaagcactt
     3241 gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg gatcggttac
     3301 aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg cccgtcagaa
     3361 ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac gttgccggac
     3421 gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc aataaaaaga
     3481 caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta atacggtatg
     3541 atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt tgtgaagtat
     3601 tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa agagggaaaa
     3661 tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca attggtttat
     3721 agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt atcaaaaacg
     3781 tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa aattatcgga
     3841 gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg atggtacagt
     3901 aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga tgagacaaga
     3961 atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct gtacgttttg
     4021 tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg ttgcacagat
     4081 catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt cacatgtcgt
     4141 tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact ttttgcatat
     4201 catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt ccactagtgg
     4261 attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc tggctatttc
     4321 cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc gaagtttccc
     4381 aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc gtcgaatcac
     4441 gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc aacaattagc
     4501 agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat ataatccagt
     4561 cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat tttatgatgt
     4621 attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag atattgatgc
     4681 agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat aatcgtatcc
     4741 tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa ctgttcaact
     4801 taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca agaaaaaccc
     4861 tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg gttttagcaa
     4921 ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga taagtttcta
     4981 cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca tgtttaatat
     5041 catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca ttgagattca
     5101 tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt ttatcaaaca
     5161 aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca gatgctccgt
     5221 gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag tgtgccatag
     5281 cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca atgatatccg
     5341 aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc aatgcttgcg
     5401 taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct aatctatccg
     5461 tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg gataaagcat
     5521 cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct gtatcaatga
     5581 aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct tcttcgttat
     5641 agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga aaggtgaaga
     5701 ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg ccaccaccga
     5761 ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt tcaatatttc
     5821 cataagttga attacccccg taccagagac tggcgacaac ctctatttcc tgtgtcaaag
     5881 ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc agcactactt
     5941 ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt ccaatggtat
     6001 aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt tgaaacgggt
     6061 taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag gtgaacctaa
     6121 gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat aagtatccta
     6181 aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt cctcttagta
     6241 tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa ttaagattat
     6301 tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag caaaagataa
     6361 tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc ctgatctttc
     6421 ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga taaataatga
     6481 aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca
     6541 aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga
     6601 ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga
     6661 agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc
     6721 ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg
     6781 tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat
     6841 aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat
     6901 ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga
     6961 gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta
     7021 tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc
     7081 ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt
     7141 tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt actt
//
LOCUS       DACEJR010000213         5741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.124, whole genome shotgun sequence.
ACCESSION   DACEJR010000213 DACEJR010000000
VERSION     DACEJR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.124"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(19..234)
                     /locus_tag="IW651_003074"
     CDS             complement(19..234)
                     /locus_tag="IW651_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0815705.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQGSVAKF"
     gene            complement(777..2927)
                     /locus_tag="IW651_003075"
                     /pseudo
     CDS             complement(777..2927)
                     /locus_tag="IW651_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3073..3648
                     /locus_tag="IW651_003076"
     CDS             3073..3648
                     /locus_tag="IW651_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0815706.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3862..4557
                     /gene="vanR"
                     /locus_tag="IW651_003077"
     CDS             3862..4557
                     /gene="vanR"
                     /locus_tag="IW651_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0815707.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4535..5689
                     /locus_tag="IW651_003078"
     CDS             4535..5689
                     /locus_tag="IW651_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0815708.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1795 a         1104 c         1087 g         1755 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc tgttcaaaat ctaaaatcaa
       61 cacatcatgg ggagtgtgat aataaatatt ttcccttaat gtaccaacaa tggtaattcg
      121 gtcaattgac cattgcattt ttaagggggt gcacatagtt tgtgcattta accgcctgtt
      181 agaaatacgg ttaagttttt cccactcatt tacgattatg ccttttttac tcaaaaatag
      241 ctcctcctca acgaaaaaag agattcttgg aaagaatttc cagagttcac gtaactctaa
      301 aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa aatattcgta taatgaactt
      361 gagttacgga actaggtgaa gtagtttccg taaaggtcga gagtgcgtca acactgctcg
      421 actatttttt tactcttttt tctcattttc tttgattata gcactcctct agaaaaaata
      481 aaaagacctc agggaatttc attccccgaa ccccttgagc ctcctcctgc cgaaatagaa
      541 tggcgctgcc agcatttatt tcgttcagct atcggctaca caatctaaca cgaccgttcc
      601 gctaccagtc gccttacagg ctccattttc gctcctctaa cgtgtttcac cgattgtgcc
      661 gataactaaa cgaaataaac gctaaaaccg ctcagaacgc aaaatatgcc attttagagc
      721 tattgtagaa aatatagttt gaaatgcaat tttcttcttt cagcttttta tttcccactt
      781 gtaaatcggt agatggtggc tccttttcca gttccataat gtggatttgc atctgcatgt
      841 agtgatgaaa cacctagctg cattctcata ccatctgacg aagatgttgt accgtcgccc
      901 caatagaaag gcaattgtaa tttatgatga aagtttacta atatggcttg ggctttattc
      961 atggcatctt catacatgcg ccattgagat acattggcta gttgcttata tgtaagtccg
     1021 ggtgtggctt cggccatctt gctcaagcca atattcattc ccattcctaa aagggcagcc
     1081 atgataatga ttgtttcttc cttatctggt tttcgattat tggaagcatg agtgaattgc
     1141 tcatgaaatc ctgttatatg ggccacatcc atgagtaaat cagttaattt tattcttggt
     1201 agcatctgat aaaggcttgc actaaatttt tttgcttctt ctggaacatc tttttctaag
     1261 cgtgcaagtg atagctttcc tttttcaaga gaaaccccat ctaacttatt ggaattggca
     1321 gctaaccact ttaacctttc attaaagctg ctggttctct ccgttatata atcttcgaat
     1381 gataaactaa ctgataatct cgtattcccc ttcgattgat tccatgtatc ttccgaaaac
     1441 aaatattcct caaaatccct atattgtctg ctgccaacaa tggaaacatc tcctgcccga
     1501 acatgctccc gaagttctgt taaaacagcc atttcatagt aatgacgatt aattgttgta
     1561 ccatcatcct cgtataaatg tcttttccat cgttttgaaa taaaatccac aggtgagtca
     1621 tcaggcactt ttcgctttcc agattcgttc attcctcgga taatctcaac agcttgtaaa
     1681 agtggctcat ttgcctttgt agaatgaaat tccaatactc ttaatagcgt tggcgtatat
     1741 tttcttagtg aataaaaccg tttttgcagt aagtctaaat aatcatagtc ggcaggacgt
     1801 gcaagttcct gagcctcttc tactgaagag acaaaggtat tccattcaat aaccgattct
     1861 aaaaccttaa aaacgtctaa tttttcctct cttgctttaa ttaatgcttg tccgatgttc
     1921 gtaaagtgta taactttctc atttagcttt ttaccgtttt gtttctggat ttcctcttga
     1981 gccttacgac cttttgataa caaactaagt atttgcctat catgaatttc aaacgcttta
     2041 tccgttagct cctgagtaag ttgtaataaa tagatggtta atatcgaata acgtttattt
     2101 tcttgaaagt cacggaatgc atacggctcg tatcttgagc ctaagcgaga cagctgcaac
     2161 aggcggttac ggtgcaaatg actaatttgc actgtttcta aatccattcc tcgtatgtat
     2221 tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag gatgacccgg tggctctttt
     2281 aaccaaccca atatcgtttt attggattcg gatggatgct gcgaggtaat aatcccttca
     2341 agcttttctt tttgctcatt tgttagagat ttactaaccg tattaaatag cttcttttca
     2401 gccattgccc ttgcttccca caccattctt tcaagtgtag tgatagcagg cagtataatt
     2461 ttgttttttc ttagaaaatc tatgcattca tgcagtagat gaatggcatc accattttcc
     2521 aaagctaatt gatgaaggta cttaaatgtc attcgatatt cactcagggt aaaagttaca
     2581 aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa gtgtattttc cctttgagga
     2641 taatgatcaa gcgaggatgg actaacacca atctgtttcg atatatattg tatgaccgaa
     2701 tctgggatgc ttttgatatg agtgtatggc caaccgggat accgaagaac agctaattga
     2761 acagcaaatc ctaaacggtt ttcttccctc cttcgcttat taactatttc taaatcccgt
     2821 ttggaaaaag tgaagtaggt ccccagtatc cattcatctt cagggatttg cataaaagcc
     2881 tgtctctgtt ccggtgtaag caattctcta cctctcgcaa ttttcattca gtatcattcc
     2941 atttctgtat tttcaattta ttagttcaat tatatatcaa tagagtgtac tctattgata
     3001 caaatgtagt agactgataa aatcatagtt aagagcgtct cataagactt gtctcaaaaa
     3061 tgaggtgata ttttgcggaa aatcggttat attcgtgtca gttcgactaa ccagaatcct
     3121 tcaagacaat ttcagcagtt gaacgagatc ggaatggata ttatatatga agagaaagtt
     3181 tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt tagacgattt acaggaagat
     3241 gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga tctatttgaa
     3301 ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga tacatggctt
     3361 gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc tggtgttaac
     3421 caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt ggctaagaaa
     3481 gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg aatgaattat
     3541 gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga aattactaat
     3601 gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc attctgtatt
     3661 ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta acagcctcct
     3721 agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa ttcttagtca
     3781 tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt aagggcaaac
     3841 ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa
     3901 attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat
     3961 accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac
     4021 atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc
     4081 tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca
     4141 atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta
     4201 aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt
     4261 atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag
     4321 cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg
     4381 aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag
     4441 agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt
     4501 gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa
     4561 aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt
     4621 agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat
     4681 cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga aattatatca
     4741 atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca ttagtattct
     4801 tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga taaataccgg
     4861 cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg aaatggatgt
     4921 tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc aggatgcaaa
     4981 gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc
     5041 ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca tgccggtaga
     5101 tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg aacagctaat
     5161 cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa caaaaacgca
     5221 catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc agctttccgc
     5281 acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg gcgaccctga
     5341 taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca gtgaggataa
     5401 cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa
     5461 cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct ataggctgga
     5521 caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat
     5581 tattgttcag catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag
     5641 ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag agatgtatat
     5701 aattttggct ctttgtcaaa tgatgttggt aaagagaaat c
//
LOCUS       DACEJR010000214         4755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.133, whole genome shotgun sequence.
ACCESSION   DACEJR010000214 DACEJR010000000
VERSION     DACEJR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.133"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..212
                     /locus_tag="IW651_003079"
     CDS             <1..212
                     /locus_tag="IW651_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0815709.1"
                     /translation="IIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQALLGTWGPTIA
                     GMAIIPSLIGAIILVLVVSFFARKL"
     gene            553..1767
                     /locus_tag="IW651_003080"
     CDS             553..1767
                     /locus_tag="IW651_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0815710.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1798..2016
                     /locus_tag="IW651_003081"
                     /pseudo
     CDS             1798..2016
                     /locus_tag="IW651_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2435..2908
                     /gene="dfrF"
                     /locus_tag="IW651_003082"
     CDS             2435..2908
                     /gene="dfrF"
                     /locus_tag="IW651_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0815711.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            3035..3691
                     /locus_tag="IW651_003083"
     CDS             3035..3691
                     /locus_tag="IW651_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0815712.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3873..4403
                     /locus_tag="IW651_003084"
     CDS             3873..4403
                     /locus_tag="IW651_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0815713.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1587 a          703 c         1007 g         1458 t
ORIGIN      
        1 gtatcattgg agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg
       61 caaacatcat tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg
      121 gaccaacaat tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat
      181 tagtcgtttc attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa
      241 tgaagaacaa atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat
      301 atgaagtatt ctttattcct tatgaaaaaa tatctttaaa aataaaaaaa gacgaaaata
      361 aacggttttc tataaaaaga agaccgttta ttttttgtct ttatttgtgt gaaaaacgaa
      421 aaagagcaca acacatcaag cttttattaa ttgtctgtct tttataaatg caagtattaa
      481 ttcaaaaaaa cagaagaaaa aaacaactac accttctttt atactcgtaa tcgtagaaag
      541 gaggcgaaaa aaatgaatac attacctatc aatacaggag atacagcatt catgatttta
      601 tgtacagcaa tggtttgcct gatgactcct ggtctagctt ttttttacgg cggattagca
      661 cggaagaata atatactaac gatcatgtct caatcttttc tctccgttgg gataacaacg
      721 attatgtggg tatttggtgg attcggattg gcctttggac gggatatcgg cggtgttatt
      781 ggaaacccgg cagatttctt tttgatgaga catgttacaa gcttccccaa catgtttcat
      841 ggtgcaacga ttccattttt aatgttcttt ctttaccaac tgatgtttat cgtgattacc
      901 gttcctttga tgacaggagc ttttgcaggg agattgaatt taaaaggcta tattatactt
      961 gttatttttt ggaatctgct gatttatttc cctgtttgcc actggatatg gggtggcgga
     1021 tttttggatc agatgggatt tagagatttc gctggtggag cagtcatcca cacgacagct
     1081 ggattcggct ctttagcatg tatcctcaca cttggtcaaa gaaagattcc tttaggagaa
     1141 agaagtcagc atagcagtct tatggcagct gctatcggta caggactgct ttggtttggc
     1201 tggttcggtt ttaattccgg tggtgcactt cgagcagatg aacaagcagt caatgcattt
     1261 ggttcaacat tcatcgctct tgcatttgcg atgattactt ggctgatcat tgcaaaagta
     1321 aaaggaaatg gctttgattt tgtcgacgta ctaacaggat ccgttgctgg attggcagcc
     1381 attacgcctt gtgccggtta tgtagaagca aaaagtgcca tgctgattgg gatcattgcg
     1441 ggtatcgtct gtcatgctgc tgttgatttt cgtaaaaaga aacaatggga tgatgcactt
     1501 gatgtgtggg gtgttcatgg catgggcgga ttcacaggta cgattctgat agggatcttt
     1561 gcatctaaca gccatttact aacgaatcca agcagctggt acttcttagg gattcaagtg
     1621 attggtgtca tcattactgc cgtgtatgcc tatgtgctga caacagtgat tttaaaatcg
     1681 gcgatgcact ttacaacgat cactactaca aaagaagaac aagaagaagg attggacttt
     1741 ttcttctttg gcaacaaact caattgaaac aaattcaaat agaaaagagg aagattcatg
     1801 caacaaagca aaacgaaaat tcctgcaaaa aaattaacgt tctttggctt tttggctatg
     1861 accatctcaa tggtcgtttc attatatgaa tatcccacat tcgcgacctc aggcttcagc
     1921 ttagtatttt tcttgttatt aggcggtttg ctatggttta tccctgtggc attgtgcgca
     1981 gcagagatgg caacggtgaa aggatgggaa aaaggtgttt cccaaacaaa aagatattaa
     2041 gaaacagccc atgtttacct gcttgcaaac aaaagaatat tgctactaga ctaagagata
     2101 ctacataagt gtgttggtat ctcttttttg ttgccgtgaa acagcagact atttcatttc
     2161 tgcttgatta ccttaaaact tattagtcta tggtttgtca agggcttgtt gcgtgagaaa
     2221 tgctgaatat caccctttac aaaacatagg ctgataagta acttttcaaa agcagaaaag
     2281 aaatggcaat gcttataaca atatgatata atgcagatga agacaaaaaa ggaatttgtc
     2341 gagctggtta gttcgagata attttgagtt tgttaagatg tggtgcactt gatggaatca
     2401 caggtacgac atcatattat ggaggatgaa atagatgata ggtttgattg ttgcgaggtc
     2461 aaagaataat gttataggca agaatggtaa tataccatgg aaaataaagg gagaacaaaa
     2521 gcaatttaga gagttaacaa cgggtaatgt ggttattatg gggcgaaagt cttatgaaga
     2581 aatcggtcat ccgttgccta atagaatgaa tattgttgtt tccaccacaa cagagtatca
     2641 aggagataat ttagtttcag ttaaatcatt agaagatgca ttattattgg ctaaaggacg
     2701 agatgtatac atatctggtg gatatggact atttaaggaa gctttgcaaa tagtagataa
     2761 aatgtatatc acagaagtag atttaaatat tgaagatgga gatacattct ttccagaatt
     2821 tgatatcaat gattttgaag ttttgatagg ggaaacactt ggtgaggaag tgaaatatac
     2881 gagaacattt tatgtaagga aaaaatgaat tgagtaaatt ttggatttag ggtgttttca
     2941 taaatatatt gctttttatg catatataaa ttgttgtaag acaaattcca gtttgtatgg
     3001 ggcgataaaa tcagaatttg agagaggatt gaatatgaat aaggaatggt ctgaacttaa
     3061 taaaacaatg caggcacaaa taaaaaagaa agatacttat aagaggggta ttgatacttt
     3121 acttacttta cgaagtcagt taatacaaac cttagtatct ttcaaagaag aattatgcag
     3181 agaagatttt aactcaatcc ccttcataaa cgctgatggt tatcatagta agacgattgc
     3241 ttattctatt tggcatattt tccgcattga agatatagtt gtgcatacag tgataaatga
     3301 agatgaacaa gtattttttg caggcaatta tcaagagcgt atcaattcac ctatcattac
     3361 aacaggaaat gaactcatga aacagcagat tgcagacttt tcaaaacagc ttaatctgga
     3421 agaattatat ttatatattt tcgaagtatg ggaaagcact gagaaaatgc ttgaacggtt
     3481 atcttatgat gaattgaaaa gaaaaatacc gaaagaaaga aaaggatact tagaatcgtt
     3541 gaatgtagta aacgacaatg aaaaagcaat ttggctgatt gattattggt gtaataaaga
     3601 tatttgtgga ctaattcaaa tgccgttttc aagacattgg attatgcaca cagaagcctg
     3661 tttgcgtata aagaacaaga tacattcata ggcaaaaatt tcagtttgta gaactaagga
     3721 aattggaatt taaggaggaa taaagtatgg ttagtgattt tataagagca gcatttccat
     3781 ggattttaat gggtttattt gtagcaatca gttgttccct tatgagtaaa aaagagaaat
     3841 aaattcctgt ttgtatttat aaaagagaaa aaatgagaaa agtagtttta tttattgcca
     3901 tgagccttga cggatatatt gcggacggta atggtggagt agcttggcta aatggtcatg
     3961 gaaatgacaa tgaaaacatc gacacttata cagaatttac caaagatata gatactgtct
     4021 taatggggtg gaatacttct catcaggttg ttactgaact ttctccgcaa gaatgggtat
     4081 ataataaatt tacaacttat gtattaacac ataaagagtg caattcttcc caacaaattc
     4141 attttacaag tgaaaatcct gttttattat tggaacggct aaaacaagaa gcaggaaagg
     4201 atatttggat ttgtggcgga gcaaatcttg ttcagcagtt ggtaagcaaa aatatgattg
     4261 ataaatatta catttctgtt attcctactc tgttaggaaa tggggttcgt cttttgggca
     4321 acatagacag ggaaataaag ttaaggcttt gtaaaacaca gacttataac ggaataaccg
     4381 atttgattta tatgcgtaga taactagaga gaaacagaaa agctgttaaa ccgacggagc
     4441 gatccgacca cgttggcggg aaaatcctta agatttttcc gccatgcttg caagggggcg
     4501 gttcgcttcg gcggttcggc gagcttgcga gccgttcaaa gcccccgtta tctaacaagg
     4561 ggggcacaaa aaacaagaaa cactatacac taacaagcgg aaaacgctgt atttataagg
     4621 caaaacccca tttttacagt agtgatataa attgtaccta tatcactatg aaaattgaat
     4681 agtaaatatg agaaagacag ttgatttcaa aaaataggct ctttgtcaat aaggactgat
     4741 gggttgtaca aaatc
//
LOCUS       DACEJR010000215         2999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.157, whole genome shotgun sequence.
ACCESSION   DACEJR010000215 DACEJR010000000
VERSION     DACEJR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.157"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW651_003085"
     CDS             245..1213
                     /locus_tag="IW651_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0815714.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW651_003086"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW651_003086"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0815715.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW651_003087"
     CDS             2243..2851
                     /locus_tag="IW651_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0815716.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          722 g          809 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DACEJR010000216         2789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.159, whole genome shotgun sequence.
ACCESSION   DACEJR010000216 DACEJR010000000
VERSION     DACEJR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.159"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            342..1253
                     /locus_tag="IW651_003088"
     CDS             342..1253
                     /locus_tag="IW651_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0815717.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1406..1891
                     /gene="vanZ"
                     /locus_tag="IW651_003089"
     CDS             1406..1891
                     /gene="vanZ"
                     /locus_tag="IW651_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0815718.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2178..2507)
                     /locus_tag="IW651_003090"
     CDS             complement(2178..2507)
                     /locus_tag="IW651_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0815719.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT          956 a          422 c          501 g          910 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc ttgcggtcat tatctgcgtg
       61 cgctgcggca agatagcctg ataataagac tgatcgcata gaggggtggt atttcacacc
      121 gcccattgtc aacaggcagt tcagcctcgt taaattcagc atgggtatca cttatgaaaa
      181 ttcatctaca ttggtgataa tagtaaatcc agtagggcga aataattgac tgtaatttac
      241 ggggcaaaac ggcacaatct caaacgagat tgtgccgttt aaggggaaga ttctagaaat
      301 atttcatact tccaactata tagttaagga ggagactgaa aatgaagaag ttgttttttt
      361 tattgttatt gttattctta atatacttag gttatgacta cgttaatgaa gcactgtttt
      421 ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc caaagaacat ttagaaaata
      481 gtgggacttc tgaaaatacc caagagaaaa caattacaga agaacaggtt tatcaaggaa
      541 atctgctatt aatcaatagt aaatatcctg ttcgccaaga aagtgtgaag tcagatatcg
      601 tgaatttatc taaacatgac gaattaataa atggatacgg gttgcttgat agtaatattt
      661 atatgtcaaa agaaatagca caaaaatttt cagagatggt caatgatgct gtaaagggtg
      721 gcgttagtca ttttattatt aatagtggct atcgagactt tgatgagcaa agtgtgcttt
      781 accaagaaat gggggctgag tatgccttac cagcaggtta tagtgagcat aattcaggtt
      841 tatcactaga tgtaggatca agcttgacga aaatggaacg agcccctgaa ggaaagtgga
      901 tagaagaaaa tgcttggaaa tacgggttca ttttacgtta tccagaggac aaaacagagt
      961 taacaggaat tcaatatgaa ccatggcata ttcgctatgt tggtttacca catagtgcga
     1021 ttatgaaaga aaagaatttc gttctcgagg aatatatgga ttacctaaaa gaagaaaaaa
     1081 ccatttctgt tagtgtaaat ggggaaaaat atgagatctt ttattatcct gttactaaaa
     1141 ataccaccat tcatgtgccg actaatcttc gttatgagat atcaggaaac aatatagacg
     1201 gtgtaattgt gacagtgttt cccggatcaa cacatactaa ttcaaggagg taaggatggc
     1261 ggaatgaaac caacgaaatt aatgaacagc attattgtac tagcactttt ggggtaacgt
     1321 tagcttttta atttaaaacc cacgttaact aggacattgc tatactaatg atacaactta
     1381 aacaaaagaa ttagaggaaa ttatattggg aaaaatatta tctagaggat tgctagcttt
     1441 atatttagtg acactaatct ggttagtgtt attcaaatta caatacaata ttttatcagt
     1501 atttaattat catcaaagaa gtcttaactt gactccattt actgctactg ggaatttcag
     1561 agagatgata gataatgtta taatctttat tccatttggc ttgcttttga atgtcaattt
     1621 taaagaaatc ggatttttac ctaagtttgc ttttgtactg gttttaagtc ttacttttga
     1681 aataattcaa tttatcttcg ctattggagc gacagacata acagatgtaa ttacaaatac
     1741 tgttggaggc tttcttggac tgaaattata tggtttaagc aataagcata tgaatcaaaa
     1801 aaaattagac agagttatta tttttgtagg tatacttttg ctcgtattat tgctcgttta
     1861 ccgtacccat ttaagaataa attacgtgta agatgtctaa atcaagcaat ctgatctttc
     1921 atacacataa agatattgaa tgaattggat tagatggaaa acgggatgtg gggaaactcg
     1981 cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta aaaagcttac ctaacactat
     2041 agtaacaaag aaagcccaat tatcaatttt agtgctgagg aattggtctc tttaataaat
     2101 ttccttaacg ttgtaaatcc gcattttcct gacggtaccc ccaaactaag atttcataga
     2161 tagattttct gataacttta aaaaacacga atagcggaaa ggatgtcttc attcgctaaa
     2221 gagtaataaa ctacttttcc atcttgacgt gattttgcca ttccatgttt ttttaaaaaa
     2281 cgtaaatggt gtgacgttgt agcgacacta gcagtaagta tttctgctaa atcacagacg
     2341 cacatttcct tataggtttc taaagcatag aaaattttaa ttctagaaaa atcgctgaaa
     2401 catttcccta acacaagtaa gtttgaaaaa tctttttgat ccaatttatt ttttattcca
     2461 tttacttttt ctttatgtac gacagtcact tggcaaactt ttttcattca tctcacctca
     2521 cattcaaacg attatttgaa tgaagtatac ctatttgatc actcttttgt caatctacct
     2581 taattccaat acactattat aagcaatttc atgtattgaa gtaaacttac ttgcaagtgc
     2641 gtttttgatt ccaagttaaa aacatattat tattctacat aatttcctaa tcaaataaaa
     2701 ctcctttgaa aattgttcta aaggagtttt attattttgt atttctacta aaaatggctc
     2761 tttgtcaata aggactgatg agttgtgca
//
LOCUS       DACEJR010000217          657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E188
            SAMN07141353-rid9869423.guided.217, whole genome shotgun sequence.
ACCESSION   DACEJR010000217 DACEJR010000000
VERSION     DACEJR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141353
            Sequence Read Archive: SRR5811555
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740885.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:00:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,034
            Genes (coding)                    :: 2,951
            CDSs (with protein)               :: 2,951
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 35 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141353-rid9869423.guided.217"
                     /isolate="E188"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..616
                     /locus_tag="IW651_003091"
                     /pseudo
     CDS             1..616
                     /locus_tag="IW651_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT          271 a           56 c          118 g          212 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttgag agtgtaaaat attttgtgta aatagaaaaa aggaagt
//
